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Type six secretion system

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https://www.readbyqxmd.com/read/27856117/adaptor-proteins-of-type-vi-secretion-system-effectors
#1
Daniel Unterweger, Benjamin Kostiuk, Stefan Pukatzki
Bacteria use the type VI secretion system (T6SS) to kill neighboring cells. One key feature of the T6SS is the secretion of diverse effectors. Here, we discuss six publications that describe three superfamilies of T6SS proteins, each dedicated to mediate the secretion of cognate effectors.
November 14, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27735916/-role-of-vascular-remodeling-markers-in-the-development-of-osteoporosis-in-idiopathic-pulmonary-arterial-hypertension
#2
V A Nevzorova, E A Kochetkova, L G Ugai, Yu V Maistrovskaya, E A Khludeeva
AIM: To define the role of circulating biomarkers for the metabolism of collagen and intercellular substance and vascular remodeling in the development of osteoporosis (OP) in idiopathic pulmonary arterial hypertension (IPAH). MATERIAL AND METHODS: Functional hemodynamic parameters, bone mineral density (BMD) in the lumbar spine and femoral neck and the serum levels of matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), MMP-9/TIMP-1 complex, C-terminal telopeptide of collagen type 1 (CITP), and endothelin-1 (ET-1) were determined in 27 high-risk IPAH patients and 30 healthy volunteers...
2016: Terapevticheskiĭ Arkhiv
https://www.readbyqxmd.com/read/27686865/the-architecture-of-the-cytoplasmic-region-of-type-iii-secretion-systems
#3
Fumiaki Makino, Dakang Shen, Naoko Kajimura, Akihiro Kawamoto, Panayiota Pissaridou, Henry Oswin, Maria Pain, Isabel Murillo, Keiichi Namba, Ariel J Blocker
Type III secretion systems (T3SSs) are essential devices in the virulence of many Gram-negative bacterial pathogens. They mediate injection of protein effectors of virulence from bacteria into eukaryotic host cells to manipulate them during infection. T3SSs involved in virulence (vT3SSs) are evolutionarily related to bacterial flagellar protein export apparatuses (fT3SSs), which are essential for flagellar assembly and cell motility. The structure of the external and transmembrane parts of both fT3SS and vT3SS is increasingly well-defined...
September 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27667690/crystal-structure-of-a-type-iv-pilus-assembly-atpase-insights-into-the-molecular-mechanism-of-pilb-from-thermus-thermophilus
#4
Jordan M Mancl, Wesley P Black, Howard Robinson, Zhaomin Yang, Florian D Schubot
Type IV pili (T4P) mediate bacterial motility and virulence. The PilB/GspE family ATPases power the assembly of T4P and type 2 secretion systems. We determined the structure of the ATPase region of PilB (PilBATP) in complex with ATPγS to provide a model of a T4P assembly ATPase and a view of a PilB/GspE family hexamer at better than 3-Å resolution. Spatial positioning and conformations of the protomers suggest a mechanism of force generation. All six PilBATP protomers contain bound ATPγS. Two protomers form a closed conformation poised for ATP hydrolysis...
September 13, 2016: Structure
https://www.readbyqxmd.com/read/27662803/putative-new-groups-of-invertebrate-water-channels-based-on-the-snail-helix-pomatia-l-helicidae-mip-protein-identification-and-phylogenetic-analysis
#5
Ewa Kosicka, Daria Grobys, Hanna Kmita, Andrzej Lesicki, Joanna R Pieńkowska
Water channel proteins, classified as a family of Membrane Intrinsic Proteins (MIPs) superfamily, enable rapid movement of water and small uncharged molecules through biological membranes. Although water channel proteins are required in several important processes characteristic for the animals, such as osmoregulation, mucus secretion, or defense against desiccation, molluscs, until now, have been very poorly explored in this aspect. Therefore, we decided to study MIPs in Helix pomatia L. applied as a model in studies on terrestrial snail physiology...
December 2016: European Journal of Cell Biology
https://www.readbyqxmd.com/read/27618265/cell-class-lineage-analysis-reveals-sexually-dimorphic-lineage-compositions-in-the-drosophila-brain
#6
Qingzhong Ren, Takeshi Awasaki, Yu-Fen Huang, Zhiyong Liu, Tzumin Lee
The morphology and physiology of neurons are directed by developmental decisions made within their lines of descent from single stem cells. Distinct stem cells may produce neurons having shared properties that define their cell class, such as the type of secreted neurotransmitter. The relationship between cell class and lineage is complex. Here we developed the transgenic cell class-lineage intersection (CLIn) system to assign cells of a particular class to specific lineages within the Drosophila brain. CLIn also enables birth-order analysis and genetic manipulation of particular cell classes arising from particular lineages...
October 10, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27601571/mapping-the-regulatory-network-for-salmonella-enterica-serovar-typhimurium-invasion
#7
Carol Smith, Anne M Stringer, Chunhong Mao, Michael J Palumbo, Joseph T Wade
UNLABELLED: Salmonella enterica pathogenicity island 1 (SPI-1) encodes proteins required for invasion of gut epithelial cells. The timing of invasion is tightly controlled by a complex regulatory network. The transcription factor (TF) HilD is the master regulator of this process and senses environmental signals associated with invasion. HilD activates transcription of genes within and outside SPI-1, including six other TFs. Thus, the transcriptional program associated with host cell invasion is controlled by at least 7 TFs...
September 6, 2016: MBio
https://www.readbyqxmd.com/read/27588814/evolution-of-new-cis-regulatory-motifs-required-for-cell-specific-gene-expression-in-caenorhabditis
#8
Michalis Barkoulas, Amhed M Vargas Velazquez, Alexandre E Peluffo, Marie-Anne Félix
Patterning of C. elegans vulval cell fates relies on inductive signaling. In this induction event, a single cell, the gonadal anchor cell, secretes LIN-3/EGF and induces three out of six competent precursor cells to acquire a vulval fate. We previously showed that this developmental system is robust to a four-fold variation in lin-3/EGF genetic dose. Here using single-molecule FISH, we find that the mean level of expression of lin-3 in the anchor cell is remarkably conserved. No change in lin-3 expression level could be detected among C...
September 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27376031/the-t6sss-of-pseudomonas-aeruginosa-strain-pao1-and-their-effectors-beyond-bacterial-cell-targeting
#9
REVIEW
Thibault G Sana, Benjamin Berni, Sophie Bleves
Pseudomonas aeruginosa is an opportunistic pathogen responsible for many diseases such as chronic lung colonization in cystic fibrosis patients and acute infections in hospitals. The capacity of P. aeruginosa to be pathogenic toward several hosts is notably due to different secretion systems. Amongst them, P. aeruginosa encodes three Type Six Secretion Systems (T6SS), named H1- to H3-T6SS, that act against either prokaryotes and/or eukaryotic cells. They are independent from each other and inject diverse toxins that interact with different components in the host cell...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27337151/within-host-evolution-of-the-dutch-high-prevalent-pseudomonas-aeruginosa-clone-st406-during-chronic-colonization-of-a-patient-with-cystic-fibrosis
#10
Rosa van Mansfeld, Mark de Been, Fernanda Paganelli, Lei Yang, Marc Bonten, Rob Willems
This study investigates adaptation of ST406, a prevalent P. aeruginosa clone, present in 15% of chronically infected cystic fibrosis (CF) patients in the Netherlands, in a newly infected CF patient during three years using whole genome sequencing (WGS), transcriptomics, and phenotypic assays, including biofilm formation. WGS-based phylogeny demonstrates that ST406 is genetically distinct from other reported CF related strains or epidemic clones. Comparative genomic analysis of the early (S1) and late (S2) isolate yielded 42 single nucleotide polymorphisms (SNPs) and 10 indels and a single 7 kb genomic fragment only found in S2...
2016: PloS One
https://www.readbyqxmd.com/read/27303737/identification-and-characterization-of-putative-translocated-effector-proteins-of-the-edwardsiella-ictaluri-type-iii-secretion-system
#11
Lidiya P Dubytska, Matthew L Rogge, Ronald L Thune
Edwardsiella ictaluri, a major pathogen in channel catfish aquaculture, encodes a type III secretion system (T3SS) that is essential for intracellular replication and virulence. Previous work identified three putative T3SS effectors in E. ictaluri, and in silico analysis of the E. ictaluri genome identified six additional putative effectors, all located on the chromosome outside the T3SS pathogenicity island. To establish active translocation by the T3SS, we constructed translational fusions of each effector to the amino-terminal adenylate cyclase (AC) domain of the Bordetella pertussis adenylate cyclase toxin CyaA...
May 2016: MSphere
https://www.readbyqxmd.com/read/27028889/components-of-the-type-six-secretion-system-are-substrates-of-francisella-tularensis-schu-s4-dsba-like-fipb-protein
#12
Aiping Qin, Yan Zhang, Melinda E Clark, Emily A Moore, Meaghan M Rabideau, G Brett Moreau, Barbara J Mann
FipB, an essential virulence factor in the highly virulent Schu S4 strain of F. tularensis subsp. tularensis, shares sequence similarity with Disulfide Bond formation (Dsb) proteins, which can have oxidoreductase, isomerase, or chaperone activity. To further explore FipB's role in virulence potential substrates were identified by co-purification and 2D gel electrophoresis, followed by protein sequencing using mass spectrometry. A total of 119 potential substrates were identified. Proteins with predicted enzymatic activity were prevalent, and there were 19 proteins that had been previously identified as impacting virulence...
November 16, 2016: Virulence
https://www.readbyqxmd.com/read/26991487/chemerin-as-a-novel-crevicular-fluid-marker-of-patients-with-periodontitis-and-type-2-diabetes-mellitus
#13
Şeyma Bozkurt Doğan, Umut Ballı, Figen Öngöz Dede, Erdim Sertoğlu, Kaan Tazegül
BACKGROUND: The objectives of the present study are to: 1) determine whether gingival crevicular fluid (GCF) chemerin is a novel predictive marker for patients with chronic periodontitis (CP) with and without type 2 diabetes mellitus (t2DM); 2) analyze the relationship between chemerin and interleukin (IL)-6 in periodontally healthy individuals and in patients with CP and with and without t2DM; and 3) evaluate the effect of non-surgical periodontal therapy on GCF chemerin levels. METHODS: Eighty individuals were split into four groups: 20 who were systemically and periodontally healthy (CTRL), 20 with t2DM and periodontally healthy (DM-CTRL), 20 systemically healthy with CP (CP), and 20 with CP and t2DM (DM-CP)...
August 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/26972728/deciphering-the-regulon-of-a-gntr-family-regulator-via-transcriptome-and-chip-exo-analyses-and-its-contribution-to-virulence-in-xanthomonas-citri
#14
Xiaofeng Zhou, Qing Yan, Nian Wang
Xanthomonas contains a large group of plant-associated species, and many of them cause severe diseases on important crops worldwide. Six gluconate-operon repressor (GntR) family transcriptional regulators are predicted in Xanthomonas and one of them belonging to the YtrA subfamily plays a prominent role in bacterial virulence. However, direct targets and comprehensive regulatory profile of YtrA remain unknown. Here, we performed microarray and high-resolution ChIP-exo experiment to identify YtrA direct targets and its DNA binding motif in X...
March 12, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/26945769/highly-expressed-amino-acid-biosynthesis-genes-revealed-by-global-gene-expression-analysis-of-salmonella-enterica-serovar-enteritidis-during-growth-in-whole-egg-are-not-essential-for-this-growth
#15
Džiuginta Jakočiūnė, Ana Herrero-Fresno, Lotte Jelsbak, John Elmerdahl Olsen
Salmonella enterica serovar Enteritidis (S. Enteritidis) is the most common cause of egg borne salmonellosis in many parts of the world. This study analyzed gene expression of this bacterium during growth in whole egg, and whether highly expressed genes were essential for the growth. High quality RNA was extracted from S. Enteritidis using a modified RNA-extraction protocol. Global gene expression during growth in whole egg was compared to growth in LB-medium using DNA array method. Twenty-six genes were significantly upregulated during growth in egg; these belonged to amino acid biosynthesis, di/oligopeptide transport system, biotin synthesis, ferrous iron transport system, and type III secretion system...
May 2, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/26931172/the-type-3-effector-nopl-of-sinorhizobium-sp-strain-ngr234-is-a-mitogen-activated-protein-kinase-substrate
#16
Ying-Ying Ge, Qi-Wang Xiang, Christian Wagner, Di Zhang, Zhi-Ping Xie, Christian Staehelin
Pathogenic bacteria utilize type 3 secretion systems to inject type 3 effectors (T3Es) into host cells, thereby subverting host defense reactions. Similarly, T3Es of symbiotic nitrogen-fixing rhizobia can affect nodule formation on roots of legumes. Previous work showed that NopL (nodulation outer protein L) of Sinorhizobium(Ensifer) sp. strain NGR234 is multiply phosphorylated in eukaryotic cells and that this T3E suppresses responses mediated by mitogen-activated protein (MAP) kinase signaling in yeast (mating pheromone signaling) and plant cells (expression of pathogenesis-related defense proteins)...
April 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/26878295/development-of-a-cost-effective-vaccine-candidate-with-outer-membrane-vesicles-of-a-tola-disrupted-shigella-boydii-strain
#17
Soma Mitra, Ritam Sinha, Jiro Mitobe, Hemanta Koley
Our previous studies on outer membrane vesicles based vaccine development against shigellosis, revealed the inability of Shigella to release significant amount of vesicles naturally, during growth. Disruption of tolA, one of the genes of the Tol-Pal system of Gram negative bacterial membrane, has increased the vesicle release rate of a Shigella boydii type 4 strain to approximately 60% higher. We also noticed the vesicles, released from tolA-disrupted strain captured more OmpA protein and lipopolysaccharide, compared to the vesicles released from its wild type prototype...
April 4, 2016: Vaccine
https://www.readbyqxmd.com/read/26821324/studying-coxiella-burnetii-type-iv-substrates-in-the-yeast-saccharomyces-cerevisiae-focus-on-subcellular-localization-and-protein-aggregation
#18
María Rodríguez-Escudero, Víctor J Cid, María Molina, Jan Schulze-Luehrmann, Anja Lührmann, Isabel Rodríguez-Escudero
Coxiella burnetii is a Gram-negative obligate parasitic bacterium that causes the disease Q-fever in humans. To establish its intracellular niche, it utilizes the Icm/Dot type IVB secretion system (T4BSS) to inject protein effectors into the host cell cytoplasm. The host targets of most cognate and candidate T4BSS-translocated effectors remain obscure. We used the yeast Saccharomyces cerevisiae as a model to express and study six C. burnetii effectors, namely AnkA, AnkB, AnkF, CBU0077, CaeA and CaeB, in search for clues about their role in C...
2016: PloS One
https://www.readbyqxmd.com/read/26820513/a-comparative-analysis-of-the-molecular-features-of-manf-and-cdnf
#19
COMPARATIVE STUDY
Junpei Norisada, Yoko Hirata, Fumimasa Amaya, Kazutoshi Kiuchi, Kentaro Oh-hashi
Cerebral dopamine neurotrophic factor (CDNF) is a paralogous protein of mesencephalic astrocyte-derived neurotrophic factor (MANF). Both proteins have been reported to show a common cytoprotective effect on dopaminergic neurons as a secretory protein containing the KDEL-like motif of the ER retrieval signal at the C-terminus, RTDL in MANF and [Q/K]TEL in CDNF among many species, although functions of paralogous proteins tend to differ from each other. In this study, we focused on post-translational regulations of their retention in the endoplasmic reticulum (ER) and secretion and performed comparative experiments on characterization of mouse MANF and mouse CDNF according to our previous report about biosynthesis and secretion of mouse MANF using a NanoLuc system...
2016: PloS One
https://www.readbyqxmd.com/read/26786106/type-3-secretion-translocators-spontaneously-assemble-a-hexadecameric-transmembrane-complex
#20
Fabian B Romano, Yuzhou Tang, Kyle C Rossi, Kathryn R Monopoli, Jennifer L Ross, Alejandro P Heuck
A type 3 secretion system is used by many bacterial pathogens to inject proteins into eukaryotic cells. Pathogens insert a translocon complex into the target eukaryotic membrane by secreting two proteins known as translocators. How these translocators form a translocon in the lipid bilayer and why both proteins are required remains elusive. Pseudomonas aeruginosa translocators PopB and PopD insert pores into membranes forming homo- or hetero-complexes of undetermined stoichiometry. Single-molecule fluorescence photobleaching experiments revealed that PopD formed mostly hexameric structures in membranes, whereas PopB displayed a bi-modal distribution with 6 and 12 subunits peaks...
March 18, 2016: Journal of Biological Chemistry
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