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https://www.readbyqxmd.com/read/29774072/twist-promotes-tumor-metastasis-in-basal-like-breast-cancer-by-transcriptionally-upregulating-ror1
#1
Jingying Cao, Xin Wang, Tao Dai, Yuanzhong Wu, Meifang Zhang, Renxian Cao, Ruhua Zhang, Gang Wang, Rou Jiang, Binhua P Zhou, Jian Shi, Tiebang Kang
Rationale: Twist is a key transcription factor for induction of epithelial-mesenchymal transition (EMT), which promotes cell migration, invasion, and cancer metastasis, confers cancer cells with stem cell-like characteristics, and provides therapeutic resistance. However, the functional roles and targeted genes of Twist in EMT and cancer progression remain elusive. Methods: The potential targeted genes of Twist were identified from the global transcriptomes of T47D/Twist cells by microarray analysis. EMT phenotype was detected by western blotting and immunofluorescence of marker proteins...
2018: Theranostics
https://www.readbyqxmd.com/read/29770348/quantitative-live-cell-reporter-assay-for-noncanonical-wnt-activity
#2
Edith P Karuna, Michael W Susman, Hsin-Yi Henry Ho
Noncanonical Wnt signaling functions independently of the β-catenin pathway to control diverse developmental processes, and dysfunction of the pathway contributes to a number of human pathological conditions, including birth defects and metastatic cancer. Progress in the field, however, has been hampered by the scarcity of functional assays for measuring noncanonical Wnt signaling activity. We recently described the Wnt5a-Ror-Kif26b (WRK) reporter assay, which directly monitors a post-transcriptional regulatory event in noncanonical Wnt signaling...
March 20, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29767687/hormone-responsive-genes-in-the-shh-and-wnt-%C3%AE-catenin-signaling-pathways-influence-urethral-closure-and-phallus-growth
#3
Yu Chen, Hongshi Yu, Andrew J Pask, Asao Fujiyama, Yutaka Suzuki, Sumio Sugano, Geoff Shaw, Marilyn B Renfree
Environmental endocrine disruptors (EEDs) that affect androgen or estrogen activity may disrupt gene regulation during normal phallus development to cause hypospadias or a masculinized clitoris. We treated developing male tammar wallabies with estrogen and females with androgen from day 20-40 postpartum (pp) during the established androgen imprinting window. Administration of estrogen to males inhibited phallus elongation but had no effect on urethral closure and did not significantly depress testicular androgen synthesis...
May 14, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29765514/wnt5a-induced-cell-migration-is-associated-with-the-aggressiveness-of-estrogen-receptor-positive-breast-cancer
#4
Yoshie Kobayashi, Takayuki Kadoya, Ai Amioka, Hideaki Hanaki, Shinsuke Sasada, Norio Masumoto, Hideki Yamamoto, Koji Arihiro, Akira Kikuchi, Morihito Okada
Elevated expression of Wnt5a is associated with malignancy, cell invasion, and metastasis. The role of Wnt5a expression in breast cancer remains elusive. We investigated the significance of Wnt5a expression in breast cancer. The relationship between Wnt5a expression and clinicopathologic factors was assessed in invasive breast cancer ( n = 178) resected at Hiroshima University Hospital between January 2011 and February 2014. Wnt5a was expressed in 69 of 178 cases (39%) of invasive breast cancer and correlated strongly with estrogen receptor (ER) expression ( P < 0...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29761053/nature-derived-lignan-compound-vb-1-exerts-hair-growth-promoting-effects-by-augmenting-wnt-%C3%AE-catenin-signaling-in-human-dermal-papilla-cells
#5
Jieshu Luo, Mengting Chen, Yingzi Liu, Hongfu Xie, Jian Yuan, Yingjun Zhou, Jinsong Ding, Zhili Deng, Ji Li
Background: Vitexin is a kind of lignan compound which has been shown to possess a variety of pharmacological effects, such as anti-inflammatory, anti-oxidative and anti-cancer activities. However the effect of vitexin on hair regeneration has not been elaborated. Methods: The proliferation of human dermal papilla cells (hDPCs) was examined by cell counting and continuous cell culture after vitexin compound 1 (VB-1) was treated. The expression of lef1 , wnt5a , bmp2 , bmp4 , alpl and vcan was examined by RT-PCR...
2018: PeerJ
https://www.readbyqxmd.com/read/29755657/regulatory-effect-of-anti-gp130-functional-mab-on-il-6-mediated-rankl-and-wnt5a-expression-through-jak-stat3-signaling-pathway-in-fls
#6
Ping Miao, Xiao Wei Zhou, Ping Wang, Rong Zhao, Ninan Chen, Chao Ying Hu, Xue Hua Chen, Liu Qian, Qi Wen Yu, Ji Ying Zhang, Rong Xu, Dong Yi He, Lian Bo Xiao, Pu Li, Mason Lu, Dong Qing Zhang
We investigated the effect on rheumatoid arthritis (RA) of an anti-gp130 monoclonal antibody (mAb) and its mechanism using RA fibroblast-like synoviocytes (FLS) and a collagen antibody-induced arthritis (CAIA) mouse model. We determined the interleukin 6 (IL-6), IL-6 receptor α (IL-6Rα), gp130, receptor activator of nuclear factor κB ligand (RANKL), matrix metalloproteinase 3 (MMP3), TIMP metallopeptidase inhibitor 1 (TIMP1), and Bcl-2 levels in RA and osteoarthritis (OA) serum and synovial fluid. RA FLS were cultured with or without IL-6/IL-6Rα; WNT5A and RANKL levels were detected...
April 17, 2018: Oncotarget
https://www.readbyqxmd.com/read/29751779/essential-role-of-atp6ap2-enrichment-in-caveolae-lipid-raft-microdomains-for-the-induction-of-neuronal-differentiation-of-stem-cells
#7
Nehman Makdissy, Katia Haddad, Jeanne D'arc AlBacha, Diana Chaker, Bassel Ismail, Albert Azar, Ghada Oreibi, David Ayoub, Ibrahim Achkar, Didier Quilliot, Ziad Fajloun
BACKGROUND: The subcellular distribution of prorenin receptor and adaptor protein ATP6AP2 may affect neurogenesis. In this study, we hypothesized that ATP6AP2 expression and subcellular relocalization from caveolae/lipid raft microdomains (CLR-Ms) to intracellular sites may correlate with neuronal differentiation (Neu-Dif) of adipose-derived mesenchymal stem cells (ADSCs). METHODS: Human ADSCs isolated from 24 healthy donors and 24 patients with neurological disorders (ND) were cultured and induced for Neu-Dif...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29749985/hyperuricemia-induces-wnt5a-ror2-gene-expression-epithelial-mesenchymal-transition-and-kidney-tubular-injury-in-mice
#8
Wiwit Ananda Wahyu Setyaningsih, Nur Arfian, Efrayim Suryadi, Muhammad Mansyur Romi, Untung Tranggono, Dwi Cahyani Ratna Sari
Background: Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelial-mesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury. Methods: A hyperuricemia model was performed in male Swiss background mice (3 months old, 30-40 g) with daily intraperitoneal injections of 125 mg/kg body weight (BW) of uric acid...
March 2018: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29743880/-trypanosoma-cruzi-exploits-wnt-signaling-pathway-to-promote-its-intracellular-replication-in-macrophages
#9
Ximena Volpini, Laura F Ambrosio, Laura Fozzatti, Constanza Insfran, Cinthia C Stempin, Laura Cervi, Claudia Cristina Motran
During the acute phase of Trypanosoma cruzi infection, macrophages can act as host cells for the parasites as well as effector cells in the early anti-parasitic immune response. Thus, the targeting of specific signaling pathways could modulate macrophages response to restrict parasite replication and instruct an appropriate adaptive response. Recently, it has become evident that Wnt signaling has immunomodulatory functions during inflammation and infection. Here, we tested the hypothesis that during T. cruzi infection, the activation of Wnt signaling pathway in macrophages plays a role in modulating the inflammatory/tolerogenic response and therefore regulating the control of parasite replication...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29741358/fibrous-topography-potentiated-canonical-wnt-signaling-directs-the-odontoblastic-differentiation-of-dental-pulp-derived-stem-cells
#10
Saeed Ur Rahman, Joung-Hwan Oh, Young-Dan Cho, Shin Hye Chung, Gene Lee, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms which underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29722624/cross-talk-between-androgen-and-wnt-signaling-potentially-contributes-to-age-related-skeletal-muscle-atrophy-in-rats
#11
Petey W Mumford, Matthew A Romero, Xuansong Mao, C Brooks Mobley, Wesley C Kephart, Cody T Haun, Paul A Roberson, Kaelin C Young, Jeffrey S Martin, Joshua F Yarrow, Darren T Beck, Michael D Roberts
We sought to determine whether age-related gastrocnemius muscle mass loss was associated with parallel decrements in androgen receptor (AR) or select Wnt signaling markers. To test this hypothesis, serum free and total testosterone (TEST) as well as gastrocnemius AR and Wnt signaling markers were analyzed in male Fischer 344 rats that were 3/6/12/18 and 24 months (mo) old (n=9 per group). Free and total TEST were greatest in 6 mo rats, and AR protein and Wnt5 protein levels linearly declined with aging. There were associations between Wnt5 protein levels and relative gastrocnemius mass (r=0...
May 3, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29720747/wnt-signaling-pathway-in-uterus-of-normal-and-seminal-vesicle-excised-mated-mice-during-pre-implantation-window
#12
Zeinab Latifi, Amir Fattahi, Kobra Hamdi, Aliye Ghasemzadeh, Pouran Karimi, Hamid Reza Nejabati, Marefat Ghaffari Novin, Leila Roshangar, Mohammad Nouri
Introduction: The importance of seminal vesicle secretion and uterine Wnt signaling for uterus preparation and embryo implantation has been described. Materials and Methods: In this study, we evaluated the gene expression of Wnt ligands (Wnt4 and Wnt5a) and their corresponding receptors (Fzd2 and Fzd6) using qRT-PCR and active β-catenin protein levels using western blotting in the uterine tissue of female mice mated with intact and seminal vesicle-excised (SVX) males during the pre-implantation window...
April 2018: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/29718273/fgf23-regulates-wnt-%C3%AE-catenin-signaling-mediated-osteoarthritis-in-mice-overexpressing-high-molecular-weight-fgf2
#13
Patience Meo Burt, Liping Xiao, Marja M Hurley
Although humans with X-linked hypophosphatemia (XLH) and the Hyp mouse, a murine homologue of XLH, are known to develop degenerative joint disease, the exact mechanism which drives the osteoarthritic (OA) phenotype remains unclear. Mice that overexpress high molecular weight FGF2 isoforms (HMWTg mice) phenocopy both XLH and Hyp including OA with increased fibroblast growth factor 23 (FGF23) production in bone and serum. Since HMWTg cartilage also has increased FGF23 and there is crosstalk between FGF23-Wnt/β-catenin signaling, the purpose of this study was to determine if OA observed in HMWTg mice is due to FGF23 mediated-canonical Wnt signaling in chondrocytes given that both pathways are implicated in OA pathogenesis...
April 26, 2018: Endocrinology
https://www.readbyqxmd.com/read/29709726/rna-sequencing-reveals-significant-mirnas-in-atypical-endometrial-hyperplasia
#14
Shiqian Tang, Yinmei Dai
PURPOSE: In this paper, we aimed to investigate the miRNAs that played a regulatory role in the development of atypical endometrial hyperplasia (AEH). METHODS: RNA sequencing was performed for endometrial tissues from 3 AEH patients and 3 endometrial normal hyperplasia patients. RNA sequencing data were processed and differentially expressed (DE) miRNAs were identified between AEH and controls. The target genes for DE miRNAs were identified and mapped to the protein-protein interaction (PPI) network...
March 19, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29709351/expression-of-hepatic-wnt5a-and-its-clinicopathological-features-in-patients-with-hepatocellular-carcinoma
#15
Li Wang, Min Yao, Miao Fang, Wen-Jie Zheng, Zhi-Zhen Dong, Liu-Hong Pan, Hai-Jian Zhang, Deng-Fu Yao
BACKGROUD: Wingless-type MMTV integration site family member 5a (Wnt5a) is involved in carcinogenesis. However, little data are available in Wnt5a signaling with hepatocellular carcinoma (HCC). In the present study, we investigated the expression of hepatic Wnt5a in HCC and the role of Wnt5a in HCC progression and outcome. METHODS: Wnt5a expression and cellular distribution in HCCs and their matched paracancerous tissues from 87 patients were analyzed with tissue microarray and immunohistochemistry and compared with hepatic Wnt3a signaling...
March 13, 2018: Hepatobiliary & Pancreatic Diseases International: HBPD INT
https://www.readbyqxmd.com/read/29705272/hsa-mir-93-regulates-mucin-family-gene-expression-via-wnt-%C3%AE-catenin-pathway-in-intrahepatic-stone-disease
#16
Hua Ma, Wenyi Li, Pinduan Bi, Qiang Wang, Jian Li, Bin Yang
BACKGROUND: Mucin plays an essential role in the intrahepatic stone formation, but the mechanism of mucin regulation is unclear. OBJECTIVE: To investigate the potential implication of miR-93 and WNT pathway in the regulation of intrahepatic bile duct mucin expression. METHODS: Thirty patients with or without intrahepatic bile duct stones are involved; Reverse-transcription polymerase chain reaction was performed to evaluate the expression of MUC3, MUC4, MUC5B, MUC5AC mRNA and miR-93 levels...
April 25, 2018: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/29704505/research-on-epigenetic-mechanism-of-sfrp2-in-advanced-chronic-myeloid-leukemia
#17
Ziye Li, Jianmin Luo
Secreted frizzled-related protein 2 (SFRP2) has been reported to act as a tumor suppressors. This study aims to detect the biological role of SFRP2 in advanced chronic myeloid leukemia (CML). In this study we examined bone marrow samples from 45 CML patients and 10 healthy donors. K562 and KCL22 cells were cultured and treated with demethylation drug and histone deacetylase inhibitor (HDACi). KCL22 and K562 cells were transfected with lentiviral vector (LV)-SFRP2, LV-control. The cells were then subjected to proliferation and apoptosis assays, real time polymerase chain reaction (PCR), Methylation-specific PCR (MSP), Western blotting, co-immunoprecipitation (CoIP) and Chromatin immunoprecipitation (ChIP), We found that SFRP2 was down-regulated in the accelerated and blast phase of CML, whereas, the levels of WNT1, WNT3 and WNT5A were up-regulated in the accelerated and blast phase of CML...
April 25, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29686674/wnt5a-signaling-promotes-defense-against-bacterial-pathogens-by-activating-a-host-autophagy-circuit
#18
Suborno Jati, Suman Kundu, Arijit Chakraborty, Sushil Kumar Mahata, Victor Nizet, Malini Sen
Bacterial pathogens are associated with severe infections (e.g., sepsis) and exacerbation of debilitating conditions such as chronic obstructive pulmonary disease (COPD). The interactions of bacterial pathogens with macrophages, a key component of innate immunity and host defense, are not clearly understood and continue to be intensively studied. Having previously demonstrated a role of Wnt5A signaling in phagocytosis, we proceeded to decipher the connection of Wnt5A signaling with infection by pathogenic bacteria, namely Pseudomonas aeruginosa (PA) and Streptococcus pneumoniae (SP), which are related with the progression of COPD and sepsis...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29680724/human-dental-pulp-cells-express-cellular-markers-for-inflammation-and-hard-tissue-formation-in-response-to-bacterial-information
#19
Akina Sugiuchi, Yousuke Sano, Masahiro Furusawa, Shu Abe, Takashi Muramatsu
INTRODUCTION: Lipopolysaccharide (LPS) is a major component of the outer membranes of gram-negative bacteria associated with deep dental caries and pulpitis. When bacteria invade dentinal tubes and dentin is continually destroyed, tertiary dentin is formed by preexisting odontoblasts. However, the relationship between LPS and tertiary dentin formation remains unclear. We investigated whether LPS stimulation induces the formation of hard tissue in human dental pulp cells (hDPCs). METHODS: Immortalized hDPCs were cultured, and Escherichia coli-derived LPS (1 μg/mL) was incorporated into the culture medium...
April 18, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29678828/wnt5a-induces-ror1-to-recruit-dock2-to-activate-rac1-2-in-chronic-lymphocytic-leukemia
#20
Md Kamrul Hasan, Jian Yu, George F Widhopf, Laura Z Rassenti, Liguang Chen, Zhouxin Shen, Steven P Briggs, Donna S Neuberg, Thomas J Kipps
Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is an oncoembryonic protein expressed on chronic lymphocytic leukemia (CLL) that can serve as a receptor for Wnt5a, which can promote leukemia-cell migration, proliferation, and survival. We found Wnt5a could induce ROR1 to complex with DOCK2 and induce activation of Rac1/2; these effects could be blocked by cirmtuzumab, a humanized anti-ROR1 mAb. We find that silencing DOCK2 specifically impaired the capacity of Wnt5a to induce activation of Rac1/2 or enhance CLL-cell proliferation...
April 20, 2018: Blood
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