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nerve conduit

Yi Cui, Yao Yao, Yannan Zhao, Zhifeng Xiao, Zongfu Cao, Sufang Han, Xing Li, Yong Huan, Juli Pan, Jianwu Dai
Numerous studies have focused on the development of novel and innovative approaches for the treatment of peripheral nerve injury using artificial nerve guide conduits. In this study, we attempted to bridge 3.5-cm defects of the sciatic nerve with a longitudinally-oriented collagen conduit (LOCC) loaded with human umbilical cord mesenchymal stem cells (hUC-MSCs). The LOCC contains a bundle of longitudinally-aligned collagenous fibers enclosed in a hollow collagen tube. Our previous studies showed that an LOCC combined with neurotrophic factors enhances peripheral nerve regeneration...
March 2, 2018: Journal of Tissue Engineering and Regenerative Medicine
Baihua Zhang, Junliang Ma, Xinjian Yan, Xu Li, Qin Xiao, Wenxiang Wang, Yong Zhou
RATIONALE: Minimally invasive esophagectomy (MIE) have been increasingly used and are regarded as suitable alternatives to open esophagectomy. However, few previous reports described minimally invasive esophagectomy using a left-sided approach. PATIENT CONCERNS AND DIAGNOSES: A 71-year-old man was admitted to our hospital because of progressive dysphagia. Synchronous double primary thoracic esophageal and left lung cancers were considered before the operation. INTERVENTIONS AND OUTCOMES: A lobectomy and MIE, via a left video-assisted thoracoscopic approach, was performed...
January 2018: Medicine (Baltimore)
Dan Liu, Daguo Mi, Tuanjie Zhang, Yanping Zhang, Junying Yan, Yaxian Wang, Xuefeng Tan, Ying Yuan, Yumin Yang, Xiaosong Gu, Wen Hu
The repair of peripheral nerve laceration injury to obtain optimal function recovery remains a big challenge in the clinic. Misdirection of regenerating axons to inappropriate target, as a result of forced mismatch of endoneurial sheaths in the case of end-to-end nerve anastomosis or nerve autografting, represents one major drawback that limits nerve function recovery. Here we tested whether tubulation repair of a nerve defect could be beneficial in terms of nerve regeneration accuracy and nerve function. We employed sequential retrograde neuronal tracing to assess the accuracy of motor axon regeneration into the tibial nerve after sciatic nerve laceration and entubulation in adult Sprague-Dawley rats...
February 21, 2018: Scientific Reports
Ching-Wen Li, Brett Davis, Jill Shea, Himanshu Sant, Bruce Kent Gale, Jayant Agarwal
Nerve conduits have been a viable alternative to the 'gold standard' autograft for treating small peripheral nerve gap injuries. However, they often produce inadequate functional recovery outcomes and are ineffective in large gap injuries. Ridge/groove surface micropatterning has been shown to promote neural cell orientation and guide growth. However, optimization of the ratio of ridge/groove parameters to promote orientation and extension for dorsal root ganglion (DRG) cells on poly(lactic-co-glycolic acid) (PLGA) films has not been previously conducted...
January 2018: Neural Regeneration Research
Anne Bolleboom, Godard C W de Ruiter, J Henk Coert, Bastiaan Tuk, Jan C Holstege, Johan W van Neck
OBJECTIVE Traumatic neuromas may develop after nerve injury at the proximal nerve stump, which can lead to neuropathic pain. These neuromas are often resistant to therapy, and excision of the neuroma frequently leads to recurrence. In this study, the authors present a novel surgical strategy to prevent neuroma formation based on the principle of centro-central anastomosis (CCA), but rather than directly connecting the nerve ends to an autograft, they created a loop using a 3D-printed polyethylene Y-shaped conduit with an autograft in the distal outlets...
February 9, 2018: Journal of Neurosurgery
Martin Koller
Polyhydroxyalkanoates (PHA) are bio-based microbial biopolyesters; their stiffness, elasticity, crystallinity and degradability are tunable by the monomeric composition, selection of microbial production strain, substrates, process parameters during production, and post-synthetic processing; they display biological alternatives for diverse technomers of petrochemical origin. This, together with the fact that their monomeric and oligomeric in vivo degradation products do not exert any toxic or elsewhere negative effect to living cells or tissue of humans or animals, makes them highly stimulating for various applications in the medical field...
February 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Yanan Zhao, Hongyu Liu, Zijian Wang, Qiang Zhang, Yinping Li, Weiqun Tian, Zan Tong, Yingying Wang, Céline Huselstein, Xiaowen Shi, Yun Chen
A series of electrodeposited chitosan-based multi-channel conduits (ECMC) with potential for peripheral nerve tissue engineering were constructed using a novel electrodeposition method combined with homemade molds. The structural and mechanical properties of the ECMC were characterized by scanning electron microscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction patterns and mechanical testing. The results showed that the electrodeposition process did not change the chemical structure of the chitosan molecules, but endowed the ECMC with high levels of flexibility and elasticity...
January 3, 2018: Colloids and Surfaces. B, Biointerfaces
Domenica Convertino, Stefano Luin, Laura Marchetti, Camilla Coletti
Graphene displays properties that make it appealing for neuroregenerative medicine, yet its interaction with peripheral neurons has been scarcely investigated. Here, we culture on graphene two established models for peripheral neurons: PC12 cells and DRG primary neurons. We perform a nano-resolved analysis of polymeric coatings on graphene and combine optical microscopy and viability assays to assess the material cytocompatibility and influence on differentiation. We find that differentiated PC12 cells display a remarkably increased neurite length on graphene (up to 27%) with respect to controls...
2018: Frontiers in Neuroscience
M D Sarker, Saman Naghieh, Adam D McInnes, David J Schreyer, Xiongbiao Chen
Nerve guidance conduits (NGCs) have been drawing considerable attention as an aid to promote regeneration of injured axons across damaged peripheral nerves. Ideally, NGCs should include physical and topographic axon guidance cues embedded as part of their composition. Over the past decades, much progress has been made in the development of NGCs that promote directional axonal regrowth so as to repair severed nerves. This paper briefly reviews the recent designs and fabrication techniques of NGCs for peripheral nerve regeneration...
February 3, 2018: Biotechnology Journal
Hidir Ozer, Huseyin Bozkurt, Gokan Bozkurt, Murat Demirbilek
OBJECTIVES: A number of chemical and biological factors, including mesenchymal stem cells (MSCs), have been developed to enhance nerve regeneration by introduction through a variety of nerve conduits. This study was designed to assess the efficacy of using chitosan-coated poly-3-hydroxybutyrate (PHB) nerve conduits seeded with human bone marrow-derived MSCs (hMSC-bm) to augment repair in an experimental rat model of sciatic nerve injury. METHODS: A total of 30 rats were randomly assigned to one of three groups (n = 10)...
January 31, 2018: International Journal of Neuroscience
Qunzhou Zhang, Phuong D Nguyen, Shihong Shi, Justin C Burrell, Qilin Xu, Kacy D Cullen, Anh D Le
Non-genetic induction of somatic cells into neural crest stem-like cells (NCSCs) is promising for potential cell-based therapies for post-traumatic peripheral nerve regeneration. Here, we report that human gingiva-derived mesenchymal stem cells (GMSCs) could be reproducibly and readily induced into NCSCs via non-genetic approaches. Compared to parental GMSCs, induced NCSC population had increased expression in NCSC-related genes and displayed robust differentiation into neuronal and Schwann-like cells. Knockdown of the expression of Yes-associated protein 1 (YAP1), a critical mechanosensor and mechanotransducer, attenuated the expression of NCSC-related genes; specific blocking of RhoA/ROCK activity and non-muscle myosin II (NM II)-dependent contraction suppressed YAP1 and NCSC-related genes and concurrently abolished neural spheroid formation in NCSCs...
January 25, 2018: Molecular Neurobiology
Paul A Wieringa, Ana Rita Gonçalves de Pinho, Silvestro Micera, Richard J A van Wezel, Lorenzo Moroni
Biofabrication techniques have endeavored to improve the regeneration of the peripheral nervous system (PNS), but nothing has surpassed the performance of current clinical practices. However, these current approaches have intrinsic limitations that compromise patient care. The "gold standard" autograft provides the best outcomes but requires suitable donor material, while implantable hollow nerve guide conduits (NGCs) can only repair small nerve defects. This review places emphasis on approaches that create structural cues within a hollow NGC lumen in order to match or exceed the regenerative performance of the autograft...
January 19, 2018: Advanced Healthcare Materials
Maryam Ebrahimi, Jafar Ai, Esmaeil Biazar, Somayeh Ebrahimi-Barough, Arash Khojasteh, Meysam Yazdankhah, Siavash Sharifi, Arman Ai, Saeed Heidari-Keshel
A nanofibrous silk nerve conduit has been evaluated for its efficiency based on the promotion of peripheral nerve regeneration in rats. The designed tubes with or without Schwann cells were implanted into a 10 mm gap in the sciatic nerves of the rats. Four months after the surgery, the regenerated nerves were monitored and evaluated by macroscopic assessments and histology. The results demonstrated that the nanofibrous grafts, especially in the presence of Schwann cells, enabled reconstruction of the rat sciatic nerve trunk with a restoration of nerve continuity and formation of nerve fibres with myelination...
January 16, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Elena Stocco, Silvia Barbon, Lucia Lora, Francesca Grandi, Leonardo Sartore, Cesare Tiengo, Lucia Petrelli, Daniele Dalzoppo, Pier Paolo Parnigotto, Veronica Macchi, Raffaele De Caro, Andrea Porzionato, Claudio Grandi
Surgical reconstruction of peripheral nerves injuries with wide substance-loss is still a challenge. Many studies focused on the development of artificial nerve conduits made of synthetic or biological materials but the ideal device has not yet been identified. Here, we manufactured a conduit for peripheral nerve regeneration using a novel biodegradable hydrogel we patented that is oxidized polyvinyl alcohol (OxPVA). Thus, its characteristics were compared with neat polyvinyl alcohol (PVA) and silk-fibroin (SF) conduits, through in vitro and in vivo analysis...
January 12, 2018: Scientific Reports
Xiaozhong Zhu, Haifeng Wei, Hongyi Zhu
The repair of injured peripheral nerve is still challenging for surgeons. The end-to-end and tension-free neurorrhaphy is the current gold standard for reconstruction after complete nerve transection without significant defect. The main objective of this study neurorrhaphy in digit replantation affects the sensory recovery and neuropathic pain in replanted digit. Total 101 patients who received replantation of single completely amputated digit were included for analysis in this study. In group I (n = 49), the digital nerves were repaired with end-to-end and tension-free neurorrhaphy and then wrapped into a tendon-derived collagen nerve conduit...
January 12, 2018: Scientific Reports
Fukai Ma, Feng Xu, Ronggang Li, Yongtao Zheng, Fan Wang, Naili Wei, Junjie Zhong, Qisheng Tang, Tongming Zhu, Zhifu Wang, Jianhong Zhu
Facial nerve injury caused by traffic accidents or operations may reduce the quality of life in patients, and recovery following the injury presents unique clinical challenges. Glial cell-derived neurotrophic factor (GDNF) is important in nerve regeneration; however, soluble GDNF rapidly diffuses into body fluids, making it difficult to achieve therapeutic efficacy. In this work, we developed a rat tail derived collagen conduit to connect nerve defects in a simple and safe manner. GDNF was immobilized in the collagen conduits via chemical conjugation to enable controlled release of GDNF...
January 9, 2018: Acta Biomaterialia
Runxin Li, Huawei Liu, Haitao Huang, Wenting Bi, Rongzeng Yan, Xinying Tan, Weisheng Wen, Chao Wang, Wenling Song, Yanhua Zhang, Feng Zhang, Min Hu
In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each group), in which the nerve was either not treated (control group) or treated with chitosan conduit, hyaluronic acid, or chitosan conduit coupled with hyaluronic acid following clamp injury to the sciatic nerve. The surgical sites were evaluated by assessing the sciatic functional index, the degree of scar adhesions, the numbers of myelinated nerve fibers, the average diameter of myelinated nerve fibers and the myelin sheath thickness...
January 8, 2018: Molecular Medicine Reports
Iwan Jones, Liudmila N Novikova, Lev N Novikov, Monika Renardy, Andreas Ullrich, Mikael Wiberg, Leif Carlsson, Paul J Kingham
Surgical intervention is the current gold standard treatment following peripheral nerve injury. However, this approach has limitations and full recovery of both motor and sensory modalities often remains incomplete. The development of artificial nerve grafts that either complement or replace current surgical procedures is therefore of paramount importance. An essential component of artificial grafts are biodegradable conduits and transplanted cells that provide trophic support during the regenerative process...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
Yosuke Niimi, Hajime Matsumine, Yuichi Takeuchi, Hironobu Osaki, Satoshi Tsunoda, Mariko Miyata, Masayuki Yamato, Hiroyuki Sakurai
PURPOSE: This study investigated the potential of collagen-coated polyglycolic acid (PGA) tube with interpositional jump graft (IPJG) in rat. MATERIALS AND METHODS: A total of 16 Lewis rats were used in this study. Facial nerve paralysis was created by ligating facial nerve trunk with a ligature clip. The rats were divided into 3 groups. Nerve conduit group (n = 6) were treated by IPJG with collagen-coated PGA tubes between the facial nerve trunks and the hypoglossal nerves...
January 8, 2018: Microsurgery
Changlai Zhu, Jing Huang, Chengbin Xue, Yaxian Wang, Shengran Wang, Shuangxi Bao, Ruyue Chen, Yuan Li, Yun Gu
Extracellular/acellular matrix has been attracted much research interests for its unique biological characteristics, and ACM modified neural scaffolds shows the remarkable role of promoting peripheral nerve regeneration. In this study, skin-derived precursors pre-differentiated into Schwann cells (SKP-SCs) were used as parent cells to generate acellular(ACM) for constructing a ACM-modified neural scaffold. SKP-SCs were co-cultured with chitosan nerve guidance conduits (NGC) and silk fibroin filamentous fillers, followed by decellularization to stimulate ACM deposition...
December 27, 2017: Neuroscience Research
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