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https://www.readbyqxmd.com/read/28337252/small-interfering-rna-targeting-mitochondrial-calcium-uniporter-improves-cardiomyocyte-cell-viability-in-hypoxia-reoxygenation-injury-by-reducing-calcium-overload
#1
Yuriana Oropeza-Almazán, Eduardo Vázquez-Garza, Héctor Chapoy-Villanueva, Guillermo Torre-Amione, Gerardo García-Rivas
Intracellular Ca(2+) mishandling is an underlying mechanism in hypoxia/reoxygenation (H/R) injury that results in mitochondrial dysfunction and cardiomyocytes death. These events are mediated by mitochondrial Ca(2+) (mCa(2+)) overload that is facilitated by the mitochondrial calcium uniporter (MCU) channel. Along this line, we evaluated the effect of siRNA-targeting MCU in cardiomyocytes subjected to H/R injury. First, cardiomyocytes treated with siRNA demonstrated a reduction of MCU expression by 67%, which resulted in significant decrease in mitochondrial Ca(2+) transport...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28328859/single-stage-nonintubated-uniportal-thoracoscopic-resection-of-synchronous-bilateral-pulmonary-nodules-after-coil-labeling-a-case-report-and-literature-review
#2
Miao Zhang, Tao Wang, You-Wei Zhang, Wen-Bin Wu, Heng Wang, Rong-Hua Xu
RATIONALE: Preoperative localization of small pulmonary nodules is essential for precise resection, besides, the optimal treatment for pulmonary nodules is controversial and the prognosis without surgery is uncertain. PATIENT CONCERNS: Herein we present a patient with compromised pulmonary function harboring synchronous triple ground-glass nodules located separately in different pulmonary lobes. DIAGNOSES: The pathological diagnosis of the nodules were chronic inflammation, inflammatory pseudotumor and atypical adenomatous hyperplasia, respectively...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28325874/ion-and-inhibitor-binding-of-the-double-ring-ion-selectivity-filter-of-the-mitochondrial-calcium-uniporter
#3
Chan Cao, Shuqing Wang, Tanxing Cui, Xun-Cheng Su, James J Chou
The calcium (Ca(2+)) uniporter of mitochondria is a holocomplex consisting of the Ca(2+)-conducting channel, known as mitochondrial calcium uniporter (MCU), and several accessory and regulatory components. A previous electrophysiology study found that the uniporter has high Ca(2+) selectivity and conductance and this depends critically on the conserved amino acid sequence motif, DXXE (Asp-X-X-Glu) of MCU. A recent NMR structure of the MCU channel from Caenorhabditis elegans revealed that the DXXE forms two parallel carboxylate rings at the channel entrance that seem to serve as the ion selectivity filter, although direct ion interaction of this structural motif has not been addressed...
March 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28306510/redox-control-of-mitochondrial-calcium-uptake
#4
Nicolas Demaurex, Manon Rosselin
Dong et al. (2017) establish how the mitochondrial Ca(2+) uniporter (MCU) integrates Ca(2+) and oxidative stress signals by identifying a cysteine residue that controls MCU channel activity, a mechanism causing mitochondrial Ca(2+) overload and cell death during oxidative stress.
March 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28280417/hydrogen-peroxide-attenuates-refilling-of-intracellular-calcium-store-in-mouse-pancreatic-acinar-cells
#5
Mi Na Yoon, Dong Kwan Kim, Se Hoon Kim, Hyung Seo Park
Intracellular calcium (Ca(2+)) oscillation is an initial event in digestive enzyme secretion of pancreatic acinar cells. Reactive oxygen species are known to be associated with a variety of oxidative stress-induced cellular disorders including pancreatitis. In this study, we investigated the effect of hydrogen peroxide (H2O2) on intracellular Ca(2+) accumulation in mouse pancreatic acinar cells. Perfusion of H2O2 at 300 µM resulted in additional elevation of intracellular Ca(2+) levels and termination of oscillatory Ca(2+) signals induced by carbamylcholine (CCh) in the presence of normal extracellular Ca(2+)...
March 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28279750/neurons-and-astrocytes-in-an-infantile-neuroaxonal-dystrophy-inad-mouse-model-show-characteristic-alterations-in-glutamate-induced-ca-2-signaling
#6
Mikhail Strokin, Georg Reiser
INAD (infantile neuroaxonal dystrophy, OMIM#256600), an autosomal recessive inherited degenerative disease, is associated with PLA2G6 mutations. PLA2G6 encodes Ca(2+)-independent phospholipase A2 (VIA iPLA2). However, it is unclear how the PLA2G6-mutations lead to disease. Non-canonical functions, which were suggested for VIA iPLA2, such as regulation of cellular and mitochondrial Ca(2+) are promising candidates. Therefore, we investigate glutamate (Glu)-evoked Ca(2+) signals in neurons and astrocytes in co-culture obtained from three INAD mouse model strains with Pla2g6 mutations, (i) hypomorphic Pla2g6 allele with reduced transcript levels, (ii) knocked-out Pla2g6, and (iii) (G373R)-point mutation with inactive VIA iPLA2 enzyme...
March 6, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28275495/resection-of-pulmonary-nodule-in-a-patient-with-subglottic-stenosis-under-modified-spontaneous-ventilation-anesthesia
#7
Jianfei Shen, Xuewei Chen, Lixia Liang, Kevin Phan, Jin-Shing Chen, Calvin S H Ng, Rene Horsleben Petersen, Diego González-Rivas, Jianxing He
Subglottic stenosis is an uncommon structural abnormality that can pose as a difficulty for patients undergoing surgery, and treatment is complex due to the special anatomical location. Pulmonary nodule resection in patients with subglottic stenosis is challenging and has not yet been reported. Here we present a case of pulmonary nodule resection in a patient with subglottic stenosis using uniportal thoracoscopy under spontaneous ventilation anesthesia (SVA). Compared with traditional double lumen endotracheal intubation, we believe this modified technique can significantly reduce airway trauma, and accelerate patient recovery...
February 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28275472/uniportal-video-assisted-thoracic-surgery-colorectal-lung-metastasectomy-in-non-intubated-anesthesia
#8
Vincenzo Ambrogi, Francesco Sellitri, Gianluca Perroni, Orazio Schillaci, Tommaso Claudio Mineo
BACKGROUND: More than ten years ago we started a program of video-assisted thoracic surgery (VATS) lung metastasectomy in non-intubated local anesthesia. In this study we investigated the effectiveness and long term results of this combined surgical-anesthesiological technique. METHODS: Between 2005 and 2014, 48 patients (25 men and 23 women) with pulmonary oligometastases from colorectal cancer, at the first episode, underwent VATS metastasectomy under non-intubated local anesthesia...
February 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28273446/tissue-specific-mitochondrial-decoding-of-cytoplasmic-ca-2-signals-is-controlled-by-the-stoichiometry-of-micu1-2-and-mcu
#9
Melanie Paillard, György Csordás, Gergö Szanda, Tünde Golenár, Valentina Debattisti, Adam Bartok, Nadan Wang, Cynthia Moffat, Erin L Seifert, András Spät, György Hajnóczky
Mitochondrial Ca(2+) uptake through the Ca(2+) uniporter supports cell functions, including oxidative metabolism, while meeting tissue-specific calcium signaling patterns and energy needs. The molecular mechanisms underlying tissue-specific control of the uniporter are unknown. Here, we investigated a possible role for tissue-specific stoichiometry between the Ca(2+)-sensing regulators (MICUs) and pore unit (MCU) of the uniporter. Low MICU1:MCU protein ratio lowered the [Ca(2+)] threshold for Ca(2+) uptake and activation of oxidative metabolism but decreased the cooperativity of uniporter activation in heart and skeletal muscle compared to liver...
March 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28262504/mitochondrial-ca-2-uniporter-is-a-mitochondrial-luminal-redox-sensor-that-augments-mcu-channel-activity
#10
Zhiwei Dong, Santhanam Shanmughapriya, Dhanendra Tomar, Naveed Siddiqui, Solomon Lynch, Neeharika Nemani, Sarah L Breves, Xueqian Zhang, Aparna Tripathi, Palaniappan Palaniappan, Massimo F Riitano, Alison M Worth, Ajay Seelam, Edmund Carvalho, Ramasamy Subbiah, Fabián Jaña, Jonathan Soboloff, Yizhi Peng, Joseph Y Cheung, Suresh K Joseph, Jeffrey Caplan, Sudarsan Rajan, Peter B Stathopulos, Muniswamy Madesh
Ca(2+) dynamics and oxidative signaling are fundamental mechanisms for mitochondrial bioenergetics and cell function. The MCU complex is the major pathway by which these signals are integrated in mitochondria. Whether and how these coactive elements interact with MCU have not been established. As an approach toward understanding the regulation of MCU channel by oxidative milieu, we adapted inflammatory and hypoxia models. We identified the conserved cysteine 97 (Cys-97) to be the only reactive thiol in human MCU that undergoes S-glutathionylation...
March 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28258579/water-transport-mediated-by-other-membrane-proteins
#11
Boyue Huang, Hongkai Wang, Baoxue Yang
Water transport through membrane is so intricate that there are still some debates. (Aquaporins) AQPs are entirely accepted to allow water transmembrane movement depending on osmotic gradient. Cotransporters and uniporters , however, are also concerned in water homeotatsis. Urea transporter B (UT-B) has a single-channel water permeability that is similar to AQP1. Cystic fibrosis transmembrane conductance regulator (CFTR ) was initially thought as a water channel but now not believed to transport water directly...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28253172/correction-dual-functions-of-a-small-regulatory-subunit-in-the-mitochondrial-calcium-uniporter-complex
#12
Ming-Feng Tsai, Charles B Phillips, Matthew Ranaghan, Chen-Wei Tsai, Yujiao Wu, Carole Willliams, Christopher Miller
No abstract text is available yet for this article.
March 2, 2017: ELife
https://www.readbyqxmd.com/read/28229450/endogenous-nitric-oxide-formation-in-cardiac-myocytes-does-not-control-respiration-during-%C3%AE-adrenergic-stimulation
#13
Michael Kohlhaas, Alexander G Nickel, Stefanie Bergem, Barbara Casadei, Ulrich Laufs, Christoph Maack
Endothelial nitric oxide (NO) controls cardiac oxygen (O2 ) consumption in a paracrine way by slowing respiration at the mitochondrial electron transport chain. While NO synthases (NOS) are also expressed in cardiac myocytes, it is unclear whether they control respiration in an intracrine way. Furthermore, the existence of a mitochondrial NOS is controversial. Here, by combining fluorescence imaging with electrical field stimulation, the patch-clamp method and knock-out technology, we determined the sources and consequences of intracellular NO formation during workload transitions in isolated murine and guinea pig cardiac myocytes and mitochondria...
February 22, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28208618/the-function-of-the-mitochondrial-calcium-uniporter-in-neurodegenerative-disorders
#14
REVIEW
Yajin Liao, Yuan Dong, Jinbo Cheng
The mitochondrial calcium uniporter (MCU)-a calcium uniporter on the inner membrane of mitochondria-controls the mitochondrial calcium uptake in normal and abnormal situations. Mitochondrial calcium is essential for the production of adenosine triphosphate (ATP); however, excessive calcium will induce mitochondrial dysfunction. Calcium homeostasis disruption and mitochondrial dysfunction is observed in many neurodegenerative disorders. However, the role and regulatory mechanism of the MCU in the development of these diseases are obscure...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28207450/response-to-response-patterns-to-the-electric-stimulation-of-epidural-catheters-in-pregnant-women-a-randomized-controlled-trial-of-uniport-versus-multiport-catheters
#15
Ellis Muggleton, Tuelin Muggleton
No abstract text is available yet for this article.
March 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28203416/learning-curve-and-established-phase-for-uniportal-vats-lobectomies-the-papworth-experience
#16
Benedetta Bedetti, Luca Bertolaccini, Piergiorgio Solli, Marco Scarci
BACKGROUND: Uniportal video-assisted thoracoscopy (VATS) has increasingly gained importance in the thoracic surgery scenario. The use of this technique can reduce postoperative pain and length of stay and gives the surgeon the same operative perspective as open surgery. METHODS: We retrospectively analysed the data of 73 patients who underwent uniportal VATS lobectomies from November 2014 to December 2015 in our institution. We divided the patients in two groups (group 1: first 30 patients vs...
January 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28198506/inhibition-of-mitochondrial-calcium-uptake-1-in-drosophila-neurons
#17
P G M'Angale, B E Staveley
The mitochondrial calcium uptake 1 (MICU1) is a regulatory subunit of the mitochondrial calcium uniporter that plays an important role in calcium sensing. It contains two EF-hand domains that are well conserved across diverse species from protozoa to plants and metazoans. The loss of MICU1 function in mammals is attributed to several neurological disorders that involve movement dysfunction. The CG4495 gene in Drosophila melanogaster was identified as a putative homolog of MICU1 in the HomoloGene database of the National Centre for Biotechnology Information (NCBI)...
February 8, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28194521/the-mitochondrial-ca-2-uniporter-structure-function-and-pharmacology
#18
Jyotsna Mishra, Bong Sook Jhun, Stephen Hurst, Jin O-Uchi, György Csordás, Shey-Shing Sheu
Mitochondrial Ca(2+) uptake is crucial for an array of cellular functions while an imbalance can elicit cell death. In this chapter, we briefly reviewed the various modes of mitochondrial Ca(2+) uptake and our current understanding of mitochondrial Ca(2+) homeostasis in regards to cell physiology and pathophysiology. Further, this chapter focuses on the molecular identities, intracellular regulators as well as the pharmacology of mitochondrial Ca(2+) uniporter complex.
February 14, 2017: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/28187435/glucose-transporter-glut1-expression-and-clinical-outcome-in-solid-tumors-a-systematic-review-and-meta-analysis
#19
Ji Wang, Chenyang Ye, Cong Chen, Hanchu Xiong, Binbin Xie, Jichun Zhou, Yongxia Chen, Shu Zheng, Linbo Wang
Glucose transporter 1 (GLUT1), the uniporter protein encoded by the SLC2A1 gene, is a key rate-limiting factor in the transport of glucose in cancer cells, and frequently expressed in a significant proportion of human cancers. Numerous studies have reported paradoxical evidence of the relationship between GLUT1 expression and prognosis in solid human tumors. To address this discrepancy, we conducted a thorough search of Pubmed and Web of Science for studies evaluating the expression of GLUT1 and overall survival (OS) and disease-free survival (DFS) in patients with solid cancer from 1993 to April 2016...
February 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28181698/calcium-uptake-via-mitochondrial-uniporter-contributes-to-palmitic-acid-induced-apoptosis-in-mouse-podocytes
#20
Zeting Yuan, Aili Cao, Hua Liu, Henjiang Guo, Yingjun Zang, Yi Wang, Yunman Wang, Hao Wang, Peihao Yin, Wen Peng
Podocytes are component cells of the glomerular filtration barrier, and their loss by apoptosis is the main cause of proteinuria that leads to diabetic nephropathy (DN). Therefore, insights into podocyte apoptosis mechanism would allow a better understanding of DN pathogenesis and thus help develop adequate therapeutic strategies. Here, we investigated the molecular mechanism of palmitic acid-inhibited cell death in mouse podocytes, and found that palmitic acid increased cell death in a dose- and time-dependent manner...
February 9, 2017: Journal of Cellular Biochemistry
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