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https://www.readbyqxmd.com/read/28410634/three-hundred-fifty-one-patients-with-pneumothorax-undergoing-uniportal-single-port-video-assisted-thoracic-surgery
#1
Hicham Masmoudi, Harry Etienne, Raphaëlle Sylvestre, Diane Evrard, Raphaël Ouede, Marielle Le Roux, Mihaela Giol, Jalal Assouad
BACKGROUND: Video-assisted thoracic surgery (VATS) is usually performed using three ports. Uniportal VATS has not yet been widely developed. We report our single institution experience in uniportal VATS for the surgical management of 351 patients with pneumothorax. METHODS: Between November 2009 and February 2016, we conducted a study in 351 patients treated for pneumothorax using uniportal VATS. Resection of apical bullae associated with partial pleurectomy, pleural abrasion, or talc effusion was performed...
April 12, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28396416/proteolytic-control-of-the-mitochondrial-calcium-uniporter-complex
#2
Chen-Wei Tsai, Yujiao Wu, Ping-Chieh Pao, Charles B Phillips, Carole Williams, Christopher Miller, Matthew Ranaghan, Ming-Feng Tsai
The mitochondrial calcium uniporter is a Ca(2+)-activated Ca(2+) channel complex mediating mitochondrial Ca(2+) uptake, a process crucial for Ca(2+) signaling, bioenergetics, and cell death. The uniporter is composed of the pore-forming MCU protein, the gatekeeping MICU1 and MICU2 subunits, and EMRE, a single-pass membrane protein that links MCU and MICU1 together. As a bridging subunit required for channel function, EMRE could paradoxically inhibit uniporter complex formation if expressed in excess. Here, we show that mitochondrial mAAA proteases AFG3L2 and SPG7 rapidly degrade unassembled EMRE using the energy of ATP hydrolysis...
April 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28389410/percutaneous-full-endoscopic-bilateral-lumbar-decompression-of-spinal-stenosis-through-uniportal-contralateral-approach-techniques-and-preliminary-results
#3
Hyeun-Sung Kim, Byapak Paudel, Jee-Soo Jang, Seong-Hoon Oh, Sol Lee, Jae Eun Park, Il-Tae Jang
BACKGROUND: When considering various risk factors such as age, comorbidities, and complications related to the surgical procedure itself, open surgery in degenerative spinal stenosis is likely to cause more complications. Here, we report the surgical procedure and preliminary clinical results of percutaneous endoscopic stenosis lumbar decompression (PESLD) technique using a uniportal-contralateral approach for bilateral decompression of degenerative spinal stenosis. MATERIALS AND METHODS: Electronic medical records (EMR) of 48 consecutive patients who were treated between January 2016 and August 2016 were reviewed retrospectively...
April 4, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28388446/phosphorylation-of-dynamin-related-protein-1-at-ser616-regulates-mitochondrial-fission-and-is-involved-in-mitochondrial-calcium-uniporter-mediated-neutrophil-polarization-and-chemotaxis
#4
Xianchong Zheng, Mimi Chen, Xiaojing Meng, Xinwei Chu, Chunqing Cai, Fei Zou
During an inflammatory response, polarization of neutrophils is necessary for effective chemotaxis and bacterial endocytosis. Ca(2+) uptake into mitochondria through the mitochondrial calcium uniporter (MCU) is crucial for cell metabolism, signaling and survival; however, the physiological role of MCU in human neutrophils remains unclear. Here we show that MCU is vital for the polarization and chemotaxis of neutrophils. Activation of MCU by spermine promotes neutrophil polarization and chemotaxis, whereas inhibition of MCU by Ru360 blunts both processes...
April 4, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28379365/new-technique-of-diaphragmatic-plication-by-means-of-uniportal-video-assisted-thoracoscopic-surgery
#5
Davor Stamenovic
Diaphragmatic plication has been a well employed method for the treatment of diaphragmatic eventration and/or paralysis. Uniportal thoracoscopic procedures seem to offer substantial benefit in terms of postoperative pain and cosmesis with similar outcomes as other surgical techniques. A 60-year-old female patient was referred to our clinic for the surgical treatment of diaphragmatic eventration of an unknown cause. The patient was referred to our institution from another hospital, where she had undergone a femoro-popliteal and crural bypass which was unsuccessful, requiring amputation of her leg...
April 3, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28364435/uniportal-middle-lobectomy-after-a-previous-right-upper-lobectomy
#6
Arthur Vieira, Paula Ugalde Figueroa
Video-assisted thoracoscopic surgery (VATS) using a 3.5 cm single incision (uniportal) may not only result in better pain control, earlier mobilization and shorter hospital stays, but can also provide safer and clear visualization to perform thoracoscopic dissection during complex surgeries. This is a case of a 55-year-old woman who underwent redo-thoracoscopy through uniportal approach for a middle-lobe lobectomy, after a previous right-upper lobectomy.
March 1, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28359500/uniportal-video-assisted-thoracoscopic-surgery-resection-of-a-giant-midesophageal-diverticulum
#7
Francesco Paolo Caronia, Alfonso Fiorelli, Mario Santini, Attilio Ignazio Lo Monte
We describe a new video-assisted technique for the management of a giant midesophageal diverticulum using a single 5-cm port. It maintained the same principles of the traditional open technique as diverticulectomy, myotomy, and fundoplication. The better visualization of the main esophageal body, diverticulum, and esophagogastric junction and the better alignment of the stapler cartridge to the longitudinal axis of the esophagus are all technical factors supporting our procedure. Heavily calcified mediastinal lymph nodes and diffuse pleural adhesions are the main contraindications...
April 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28351840/macrophages-utilize-the-mitochondrial-calcium-uniporter-for-profibrotic-polarization
#8
Linlin Gu, Jennifer L Larson-Casey, A Brent Carter
Fibrosis in multiple organs, including the liver, kidney, and lung, often occurs secondary to environmental exposure. Asbestos exposure is one important environmental cause of lung fibrosis. The mechanisms that mediate fibrosis is not fully understood, although mitochondrial oxidative stress in alveolar macrophages is critical for fibrosis development. Mitochondrial Ca(2+) levels can be associated with production of reactive oxygen species. Here, we show that patients with asbestosis have higher levels of mitochondrial Ca(2+) compared with normal patients...
March 28, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28345618/ptpip51-regulates-mouse-cardiac-ischemia-reperfusion-through-mediating-the-mitochondria-sr-junction
#9
Xue Qiao, Shi Jia, Jingjing Ye, Xuan Fang, Chenglin Zhang, Yangpo Cao, Chunling Xu, Lifang Zhao, Yi Zhu, Lu Wang, Ming Zheng
Protein tyrosine phosphatase interacting protein 51 (PTPIP51) participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders. However, there is no functional evidence showing the physiological or pathological roles of PTPIP51 in the heart. We have therefore investigated the role and mechanisms of PTPIP51 in regulating cardiac function. We found that PTPIP51 was markedly upregulated in ischemia/reperfusion heart. Upregulation of PTPIP51 by adenovirus-mediated overexpression markedly increased the contact of mitochondria-sarcoplasmic reticulum (SR), elevated mitochondrial Ca(2+) uptake from SR release through mitochondrial Ca(2+)uniporter...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28341416/a-physiologically-oriented-transcriptomic-analysis-of-the-midgut-of-tenebrio-molitor
#10
Nathalia R Moreira, Christiane Cardoso, Renata O Dias, Clelia Ferreira, Walter R Terra
Physiological data showed that T. molitor midgut is buffered at pH 5.6 at the two anterior thirds and at 7.9 at the posterior third. Furthermore, water is absorbed and secreted at the anterior and posterior midgut, respectively, driving a midgut counter flux of fluid. To look for the molecular mechanisms underlying these phenomena and nutrient absorption as well, a transcriptomic approach was used. For this, 11 types of transporters were chosen from the midgut transcriptome obtained by pyrosequencing (Roche 454)...
March 21, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28337252/small-interfering-rna-targeting-mitochondrial-calcium-uniporter-improves-cardiomyocyte-cell-viability-in-hypoxia-reoxygenation-injury-by-reducing-calcium-overload
#11
Yuriana Oropeza-Almazán, Eduardo Vázquez-Garza, Héctor Chapoy-Villanueva, Guillermo Torre-Amione, Gerardo García-Rivas
Intracellular Ca(2+) mishandling is an underlying mechanism in hypoxia/reoxygenation (H/R) injury that results in mitochondrial dysfunction and cardiomyocytes death. These events are mediated by mitochondrial Ca(2+) (mCa(2+)) overload that is facilitated by the mitochondrial calcium uniporter (MCU) channel. Along this line, we evaluated the effect of siRNA-targeting MCU in cardiomyocytes subjected to H/R injury. First, cardiomyocytes treated with siRNA demonstrated a reduction of MCU expression by 67%, which resulted in significant decrease in mitochondrial Ca(2+) transport...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28328859/single-stage-nonintubated-uniportal-thoracoscopic-resection-of-synchronous-bilateral-pulmonary-nodules-after-coil-labeling-a-case-report-and-literature-review
#12
Miao Zhang, Tao Wang, You-Wei Zhang, Wen-Bin Wu, Heng Wang, Rong-Hua Xu
RATIONALE: Preoperative localization of small pulmonary nodules is essential for precise resection, besides, the optimal treatment for pulmonary nodules is controversial and the prognosis without surgery is uncertain. PATIENT CONCERNS: Herein we present a patient with compromised pulmonary function harboring synchronous triple ground-glass nodules located separately in different pulmonary lobes. DIAGNOSES: The pathological diagnosis of the nodules were chronic inflammation, inflammatory pseudotumor and atypical adenomatous hyperplasia, respectively...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28325874/ion-and-inhibitor-binding-of-the-double-ring-ion-selectivity-filter-of-the-mitochondrial-calcium-uniporter
#13
Chan Cao, Shuqing Wang, Tanxing Cui, Xun-Cheng Su, James J Chou
The calcium (Ca(2+)) uniporter of mitochondria is a holocomplex consisting of the Ca(2+)-conducting channel, known as mitochondrial calcium uniporter (MCU), and several accessory and regulatory components. A previous electrophysiology study found that the uniporter has high Ca(2+) selectivity and conductance and this depends critically on the conserved amino acid sequence motif, DXXE (Asp-X-X-Glu) of MCU. A recent NMR structure of the MCU channel from Caenorhabditis elegans revealed that the DXXE forms two parallel carboxylate rings at the channel entrance that seem to serve as the ion selectivity filter, although direct ion interaction of this structural motif has not been addressed...
April 4, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28306510/redox-control-of-mitochondrial-calcium-uptake
#14
Nicolas Demaurex, Manon Rosselin
Dong et al. (2017) establish how the mitochondrial Ca(2+) uniporter (MCU) integrates Ca(2+) and oxidative stress signals by identifying a cysteine residue that controls MCU channel activity, a mechanism causing mitochondrial Ca(2+) overload and cell death during oxidative stress.
March 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28280417/hydrogen-peroxide-attenuates-refilling-of-intracellular-calcium-store-in-mouse-pancreatic-acinar-cells
#15
Mi Na Yoon, Dong Kwan Kim, Se Hoon Kim, Hyung Seo Park
Intracellular calcium (Ca(2+)) oscillation is an initial event in digestive enzyme secretion of pancreatic acinar cells. Reactive oxygen species are known to be associated with a variety of oxidative stress-induced cellular disorders including pancreatitis. In this study, we investigated the effect of hydrogen peroxide (H2O2) on intracellular Ca(2+) accumulation in mouse pancreatic acinar cells. Perfusion of H2O2 at 300 µM resulted in additional elevation of intracellular Ca(2+) levels and termination of oscillatory Ca(2+) signals induced by carbamylcholine (CCh) in the presence of normal extracellular Ca(2+)...
March 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28279750/neurons-and-astrocytes-in-an-infantile-neuroaxonal-dystrophy-inad-mouse-model-show-characteristic-alterations-in-glutamate-induced-ca-2-signaling
#16
Mikhail Strokin, Georg Reiser
INAD (infantile neuroaxonal dystrophy, OMIM#256600), an autosomal recessive inherited degenerative disease, is associated with PLA2G6 mutations. PLA2G6 encodes Ca(2+)-independent phospholipase A2 (VIA iPLA2). However, it is unclear how the PLA2G6-mutations lead to disease. Non-canonical functions, which were suggested for VIA iPLA2, such as regulation of cellular and mitochondrial Ca(2+) are promising candidates. Therefore, we investigate glutamate (Glu)-evoked Ca(2+) signals in neurons and astrocytes in co-culture obtained from three INAD mouse model strains with Pla2g6 mutations, (i) hypomorphic Pla2g6 allele with reduced transcript levels, (ii) knocked-out Pla2g6, and (iii) (G373R)-point mutation with inactive VIA iPLA2 enzyme...
March 6, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28275495/resection-of-pulmonary-nodule-in-a-patient-with-subglottic-stenosis-under-modified-spontaneous-ventilation-anesthesia
#17
Jianfei Shen, Xuewei Chen, Lixia Liang, Kevin Phan, Jin-Shing Chen, Calvin S H Ng, Rene Horsleben Petersen, Diego González-Rivas, Jianxing He
Subglottic stenosis is an uncommon structural abnormality that can pose as a difficulty for patients undergoing surgery, and treatment is complex due to the special anatomical location. Pulmonary nodule resection in patients with subglottic stenosis is challenging and has not yet been reported. Here we present a case of pulmonary nodule resection in a patient with subglottic stenosis using uniportal thoracoscopy under spontaneous ventilation anesthesia (SVA). Compared with traditional double lumen endotracheal intubation, we believe this modified technique can significantly reduce airway trauma, and accelerate patient recovery...
February 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28275472/uniportal-video-assisted-thoracic-surgery-colorectal-lung-metastasectomy-in-non-intubated-anesthesia
#18
Vincenzo Ambrogi, Francesco Sellitri, Gianluca Perroni, Orazio Schillaci, Tommaso Claudio Mineo
BACKGROUND: More than ten years ago we started a program of video-assisted thoracic surgery (VATS) lung metastasectomy in non-intubated local anesthesia. In this study we investigated the effectiveness and long term results of this combined surgical-anesthesiological technique. METHODS: Between 2005 and 2014, 48 patients (25 men and 23 women) with pulmonary oligometastases from colorectal cancer, at the first episode, underwent VATS metastasectomy under non-intubated local anesthesia...
February 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28273446/tissue-specific-mitochondrial-decoding-of-cytoplasmic-ca-2-signals-is-controlled-by-the-stoichiometry-of-micu1-2-and-mcu
#19
Melanie Paillard, György Csordás, Gergö Szanda, Tünde Golenár, Valentina Debattisti, Adam Bartok, Nadan Wang, Cynthia Moffat, Erin L Seifert, András Spät, György Hajnóczky
Mitochondrial Ca(2+) uptake through the Ca(2+) uniporter supports cell functions, including oxidative metabolism, while meeting tissue-specific calcium signaling patterns and energy needs. The molecular mechanisms underlying tissue-specific control of the uniporter are unknown. Here, we investigated a possible role for tissue-specific stoichiometry between the Ca(2+)-sensing regulators (MICUs) and pore unit (MCU) of the uniporter. Low MICU1:MCU protein ratio lowered the [Ca(2+)] threshold for Ca(2+) uptake and activation of oxidative metabolism but decreased the cooperativity of uniporter activation in heart and skeletal muscle compared to liver...
March 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28262504/mitochondrial-ca-2-uniporter-is-a-mitochondrial-luminal-redox-sensor-that-augments-mcu-channel-activity
#20
Zhiwei Dong, Santhanam Shanmughapriya, Dhanendra Tomar, Naveed Siddiqui, Solomon Lynch, Neeharika Nemani, Sarah L Breves, Xueqian Zhang, Aparna Tripathi, Palaniappan Palaniappan, Massimo F Riitano, Alison M Worth, Ajay Seelam, Edmund Carvalho, Ramasamy Subbiah, Fabián Jaña, Jonathan Soboloff, Yizhi Peng, Joseph Y Cheung, Suresh K Joseph, Jeffrey Caplan, Sudarsan Rajan, Peter B Stathopulos, Muniswamy Madesh
Ca(2+) dynamics and oxidative signaling are fundamental mechanisms for mitochondrial bioenergetics and cell function. The MCU complex is the major pathway by which these signals are integrated in mitochondria. Whether and how these coactive elements interact with MCU have not been established. As an approach toward understanding the regulation of MCU channel by oxidative milieu, we adapted inflammatory and hypoxia models. We identified the conserved cysteine 97 (Cys-97) to be the only reactive thiol in human MCU that undergoes S-glutathionylation...
March 16, 2017: Molecular Cell
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