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https://www.readbyqxmd.com/read/29323106/sequential-forward-and-reverse-transport-of-the-na-ca2-exchanger-generates-ca2-oscillations-within-mitochondria
#1
Krishna Samanta, Gary R Mirams, Anant B Parekh
Mitochondrial Ca2+ homoeostasis regulates aerobic metabolism and cell survival. Ca2+ flux into mitochondria is mediated by the mitochondrial calcium uniporter (MCU) channel whereas Ca2+ export is often through an electrogenic Na+-Ca2+ exchanger. Here, we report remarkable functional versatility in mitochondrial Na+-Ca2+ exchange under conditions where mitochondria are depolarised. Following physiological stimulation of cell-surface receptors, mitochondrial Na+-Ca2+ exchange initially operates in reverse mode, transporting cytosolic Ca2+ into the matrix...
January 11, 2018: Nature Communications
https://www.readbyqxmd.com/read/29286188/the-mitochondrial-calcium-uniporter-complex-in-trypanosomes
#2
REVIEW
Noelia Lander, Miguel A Chiurillo, Mayara S Bertolini, Roberto Docampo, Aníbal E Vercesi
The presence of a conserved mechanism for mitochondrial calcium uptake in trypanosomatids was crucial for the molecular identification of the mitochondrial calcium uniporter (MCU), a long-sought channel present in most eukaryotic organisms. Since then, research efforts to elucidate the role of MCU and its regulatory elements in different biological models have multiplied. MCU is the pore-forming subunit of a multimeric complex (the MCU complex or MCUC) and its predicted structure in trypanosomes is simpler than in mammalian cells, lacking two of its subunits and probably possessing other unidentified components...
December 29, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29241542/micu2-restricts-spatial-crosstalk-between-insp3r-and-mcu-channels-by-regulating-threshold-and-gain-of-micu1-mediated-inhibition-and-activation-of-mcu
#3
Riley Payne, Henry Hoff, Anne Roskowski, J Kevin Foskett
Ca2+ entry into mitochondria is mediated by the Ca2+ uniporter-channel complex containing MCU, the Ca2+-selective pore, and associated regulatory proteins. The roles of MICU proteins are controversial. MICU1 was proposed to be necessary for MCU activity, whereas subsequent studies suggested it inhibits the channel in the low-cytoplasmic Ca2+ ([Ca2+]c) regime, a mechanism referred to as "gatekeeping," that imposes a [Ca2+]c threshold for channel activation at ∼1-3 μM. Here, we measured MCU activity over a wide range of quantitatively controlled and recorded [Ca2+]c...
December 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/29237825/mitochondrial-ca2-transport-in-the-endothelium-regulation-by-ions-redox-signalling-and-mechanical-forces
#4
REVIEW
B Rita Alevriadou, Santhanam Shanmughapriya, Akshar Patel, Peter B Stathopulos, Muniswamy Madesh
Calcium (Ca2+) transport by mitochondria is an important component of the cell Ca2+ homeostasis machinery in metazoans. Ca2+ uptake by mitochondria is a major determinant of bioenergetics and cell fate. Mitochondrial Ca2+ uptake occurs via the mitochondrial Ca2+ uniporter (MCU) complex, an inner mitochondrial membrane protein assembly consisting of the MCU Ca2+ channel, as its core component, and the MCU complex regulatory/auxiliary proteins. In this review, we summarize the current knowledge on the molecular nature of the MCU complex and its regulation by intra- and extramitochondrial levels of divalent ions and reactive oxygen species (ROS)...
December 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29234103/an-exhaustive-search-algorithm-to-aid-nmr-based-structure-determination-of-rotationally-symmetric-transmembrane-oligomers
#5
Jing Yang, Alessandro Piai, Hong-Bin Shen, James J Chou
Nuclear magnetic resonance (NMR) has been an important source of structural restraints for solving structures of oligomeric transmembrane domains (TMDs) of cell surface receptors and viral membrane proteins. In NMR studies, oligomers are assembled using inter-protomer distance restraints. But, for oligomers that are higher than dimer, these distance restraints all have two-fold directional ambiguity, and resolving such ambiguity often requires time-consuming trial-and-error calculations using restrained molecular dynamics (MD) with simulated annealing (SA)...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232901/a-novel-passive-wireless-sensing-method-for-concrete-chloride-ion-concentration-monitoring
#6
Shuangxi Zhou, Wei Sheng, Fangming Deng, Xiang Wu, Zhihui Fu
In this paper, a novel approach for concrete chloride ion concentration measuring based on passive and wireless sensor tag is proposed. The chloride ion sensor based on RFID communication protocol is consisting of an energy harvesting and management circuit, a low dropout voltage regulator, a MCU, a RFID tag chip and a pair of electrodes. The proposed sensor harvests energy radiated by the RFID reader to power its circuitry. To improve the stability of power supply, a three-stage boost rectifier is customized to rectify the harvested power into dc power and step-up the voltage...
December 11, 2017: Sensors
https://www.readbyqxmd.com/read/29225050/inhibition-of-the-mitochondrial-calcium-uniporter-prevents-il-13-and-allergen-mediated-airway-epithelial-apoptosis-and-loss-of-barrier-function
#7
Sara C Sebag, Olha M Koval, John D Paschke, Christopher J Winters, Alejandro P Comellas, Isabella M Grumbach
Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca2+ uniporter (MCU) resides in the inner mitochondrial membrane and controls mitochondrial Ca2+ uptake into the mitochondrial matrix. To understand the function of MCU in models of allergic asthma, in vitro and in vivo studies were performed using models of functional deficiency or knockout of MCU...
January 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29210126/crosslink-of-calcium-and-sodium-signalling-in-health-and-disease
#8
Alexei Verkhratsky, Mohamed Trebak, Fabiana Perocchi, Daniel Khananishvili, Israel Sekler
Transmembrane ionic gradients, that are indispensable feature of life, are utilised for generation of cytosolic ionic signals that regulate a host of cellular functions. Intracellular signalling mediated by Ca2+ and Na+ is tightly linked through several molecular pathways that generate Ca2+ and Na+ fluxes and are in turn regulated by both ions. Transient receptor potential (TRP) channels bridge endoplasmic reticulum Ca2+ release with generation of Na+ and Ca2+ currents. Plasmalemmal Na+ /Ca2+ exchanger by flickering between forward and reverse mode coordinates influx and efflux of both ions with membrane polarisation and cytosolic ion concentration...
December 6, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/29209754/-stricture-of-the-vesicourethral-anastomosis-after-radical-prostatectomy
#9
REVIEW
V Beck, M Apfelbeck, M Chaloupka, A Kretschmer, F Strittmatter, S Tritschler
BACKGROUND: The development of a stricture of the vesicourethral anastomosis is a serious complication after radical prostatectomy. Strictures occur in 5-8% of patients after radical prostatectomy. SYMPTOMS: Usually the clinical symptoms include an irritative and obstructive component similar to benign prostatic hyperplasia. In rare cases, patients suffer from partial or complete stress incontinence as a result of the anastomotic stricture. DIAGNOSTICS: The diagnostic workup is similar to the procedure for urethral strictures...
December 5, 2017: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/29204122/letm1-mediated-k-and-na-homeostasis-regulates-mitochondrial-ca2-efflux
#10
Shane Austin, Mojtaba Tavakoli, Christina Pfeiffer, Julia Seifert, Andrea Mattarei, Diego De Stefani, Mario Zoratti, Karin Nowikovsky
HIGHLIGHTS Monovalent cation homeostasis is dysregulated upon LETM1 depletionK+/H+ exchange activity is decreased in LETM1 knockdown cellsLETM1 depletion results in K+ accumulation in the mitochondrial matrixLETM1 knockdown does not affect expression of major mitochondrial Ca2+ transport modulatorsLETM1-regulated mitochondrial Ca2+ fluxes are dependent on Na+ Ca2+ transport across the inner membrane of mitochondria (IMM) is of major importance for their functions in bioenergetics, cell death and signaling. It is therefore tightly regulated...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29188424/increase-in-mitochondrial-biogenesis-in-neuronal-cells-by-rns60-a-physically-modified-saline-via-phosphatidylinositol-3-kinase-mediated-upregulation-of-pgc1%C3%AE
#11
Goutam Chandra, Madhuchhanda Kundu, Suresh B Rangasamy, Sridevi Dasarathy, Supurna Ghosh, Richard Watson, Kalipada Pahan
This study highlights a novel approach to upregulate mitochondrial biogenesis in neuronal cells. RNS60 is a 0.9% saline solution containing oxygenated nanobubbles that is generated by subjecting normal saline to Taylor-Couette-Poiseuille (TCP) flow under elevated oxygen pressure. RNS60, but not NS (normal saline), PNS60 (saline containing a comparable level of oxygen without the TCP modification), or RNS10.3 (TCP-modified normal saline without excess oxygen), increased the expression of Nrf1, Tfam, Mcu, and Tom20 (genes associated with mitochondrial biogenesis) and upregulated mitochondrial biogenesis in MN9D dopaminergic neuronal cells...
November 29, 2017: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
https://www.readbyqxmd.com/read/29178901/bereaved-family-members-perceptions-of-the-quality-of-end-of-life-care-across-four-types-of-inpatient-care-settings
#12
Kelli Stajduhar, Richard Sawatzky, S Robin Cohen, Daren K Heyland, Diane Allan, Darcee Bidgood, Leah Norgrove, Anne M Gadermann
BACKGROUND: The aims of this study were to gain a better understanding of how bereaved family members perceive the quality of EOL care by comparing their satisfaction with quality of end-of-life care across four different settings and by additionally examining the extent to which demographic characteristics and psychological variables (resilience, optimism, grief) explain variation in satisfaction. METHODS: A cross-sectional mail-out survey was conducted of bereaved family members of patients who had died in extended care units (n = 63), intensive care units (n = 30), medical care units (n = 140) and palliative care units (n = 155)...
November 25, 2017: BMC Palliative Care
https://www.readbyqxmd.com/read/29160703/ruthenium-carbon-rich-group-as-a-redox-switchable-metal-coupling-unit-in-linear-trinuclear-complexes
#13
Emmanuel Di Piazza, Corentin Boilleau, Antoine Vacher, Khalissa Merahi, Lucie Norel, Karine Costuas, Thierry Roisnel, Sylvie Choua, Philippe Turek, Stéphane Rigaut
The preparation and properties of novel ruthenium carbon-rich complexes [(Ph-C≡C-)2-nRu(dppe)2(-C≡C-bipyM(hfac)2)n] (n = 1, 2; M = Cu(II), Mn(II); bipy = 2,2'-bipyridin-5-yl) characterized by single-crystal X-ray diffraction and designed for molecular magnetism are reported. With the help of EPR spectroscopy, we show that the neutral ruthenium system sets up a magnetic coupling between two remote paramagnetic Cu(II) units. More specifically, these copper compounds are unique examples of bimetallic and linear heterotrimetallic compounds for which a complete rationalization of the magnetic interactions could be made for exceptionally long distances between the spin carriers (8...
November 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29141899/atypical-g-protein-%C3%AE-5-promotes-cardiac-oxidative-stress-apoptosis-and-fibrotic-remodeling-in-response-to-multiple-cancer-chemotherapeutics
#14
Biswanath Maity, Sreemoyee Chakraborti, Arnab Pramanick, Sudipta Saha, Somnath Singha Roy, Arnab Ray Chaudhuri, Madhusudan Das, Sujoy Ghosh, Adele Stewart
The clinical use of multiple classes of cancer chemotherapeutics is limited by irreversible, dose-dependent, and sometimes life-threatening cardiotoxicity. We report here that, though distinct in their mechanisms of action, doxorubicin, paclitaxel, and 5-FU all induce rapid and robust upregulation of atypical G protein Gβ5 in the myocardium, correlating with oxidative stress, myocyte apoptosis, and the accumulation of pro-inflammatory and pro-fibrotic cytokines. In ventricular cardiac myocytes (VCM), Gβ5 deficiency provided substantial protection against the cytotoxic actions of chemotherapeutics, including reductions in oxidative stress and simultaneous attenuation of ROS-dependent activation of the ATM and CaMKII pro-apoptotic signaling cascades...
November 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/29137386/mitochondrial-calcium-uniporter-as-a-target-of-microrna-340-and-promoter-of-metastasis-via-enhancing-the-warburg-effect
#15
Changhui Yu, Yuhao Wang, Jiawen Peng, Qiang Shen, Mimi Chen, Wei Tang, Xiumei Li, Chunqing Cai, Bin Wang, Shaoxi Cai, Xiaojing Meng, Fei Zou
Background: A shift from oxygen phosphorylation to aerobic glycolysis was known as the Warburg effect and a characteristic of cancer cell metabolism facilitating metastasis. Mitochondrial calcium uniporter (MCU), a key ion channel that mediates Ca(2+) uptake into mitochondria, was found to promote cancer progression and metastasis. However, its explicit role in shifting metabolism of breast cancer cells has not been defined. Methods: We evaluated MCU overexpression or knock-down on migration, invasion and glucose metabolismin breast cancer cells...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29073097/content-of-mitochondrial-calcium-uniporter-mcu-in-cardiomyocytes-is-regulated-by-microrna-1-in-physiologic-and-pathologic-hypertrophy
#16
Tania Zaglia, Paola Ceriotti, Antonio Campo, Giulia Borile, Andrea Armani, Pierluigi Carullo, Valentina Prando, Raffaele Coppini, Vladimiro Vida, Tomas O Stølen, Wisløff Ulrik, Elisabetta Cerbai, Giovanni Stellin, Giuseppe Faggian, Diego De Stefani, Marco Sandri, Rosario Rizzuto, Fabio Di Lisa, Tullio Pozzan, Daniele Catalucci, Marco Mongillo
The mitochondrial Ca(2+) uniporter complex (MCUC) is a multimeric ion channel which, by tuning Ca(2+) influx into the mitochondrial matrix, finely regulates metabolic energy production. In the heart, this dynamic control of mitochondrial Ca(2+) uptake is fundamental for cardiomyocytes to adapt to either physiologic or pathologic stresses. Mitochondrial calcium uniporter (MCU), which is the core channel subunit of MCUC, has been shown to play a critical role in the response to β-adrenoreceptor stimulation occurring during acute exercise...
October 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29055103/mitochondrial-ca-2-dynamics-in-cells-and-suspensions
#17
Benjamin Wacquier, Hugo E Romero C, Virginia González-Vélez, Laurent Combettes, Geneviève Dupont
Mitochondria play a significant role in shaping cytosolic Ca(2+) signals. Thus, Ca(2+) transfers across the mitochondrial membrane are much studied, not only in intact cells but also in artificial systems such as mitochondrial suspensions or permeabilised cells. Observed rates of Ca(2+) changes vary by at least one order of magnitude. In this work, we investigate the relationship between the Ca(2+) dynamics observed in various experimental conditions using a computational model calibrated on experimental data...
October 21, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29048846/-a-novel-ship-borne-positive-pressure-solid-phase-extraction-device-to-enrich-organo-chlorinated-and-pyrethroid-pesticides-in-seawater
#18
Jianglei Ye
A novel solid phase extraction (SPE) device driven by positive pressure was developed instead of negative pressure from a vacuum pump, in order to enrich organo chlorinated and pyrethroid pesticides in seawater. The water sampling bottles and the pipelines which touch water samples were made of plastic material without chlorine. In order to ensure the sealing and firmness, the whole device were tightened with nut and bolt. The inner pressure (0.1-0.3 MPa) in the water sampling bottle was provided by the small air pump (powered by 12 V cell) controlled by a microprogrammed control unit (MCU) and pressure sensor to keep the water flow rate (4...
September 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29038245/mitochondrial-calcium-dysregulation-contributes-to-dendrite-degeneration-mediated-by-pd-lbd-associated-lrrk2-mutants
#19
Manish Verma, Jason Callio, P Anthony Otero, Israel Sekler, Zachary P Wills, Charleen T Chu
Mutations in leucine-rich repeat kinase 2 (LRRK2) contribute to development of late-onset familial Parkinson's disease (PD), with clinical features of motor and cognitive dysfunction indistinguishable from sporadic PD. Calcium dysregulation plays an important role in PD pathogenesis, but the mechanisms of neurodegeneration remain unclear. Recent reports indicate enhanced excitatory neurotransmission in cortical neurons expressing mutant LRRK2, which occurs prior to the well-characterized phenotype of dendritic shortening...
October 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28987949/zingiberis-rhizoma-mediated-enhancement-of-the-pharmacological-effect-of-aconiti-lateralis-radix-praeparata-against-acute-heart-failure-and-the-underlying-biological-mechanisms
#20
Lu Zhang, Xiaohua Lu, Jiabo Wang, Pengyan Li, Haotian Li, Shizhang Wei, Xuelin Zhou, Kun Li, Lifu Wang, Ruilin Wang, Yanling Zhao, Xiaohe Xiao
Aconiti Lateralis Radix Praeparata (Fuzi), a type of Chinese materia medica, has been used to treat acute and chronic heart failure (HF) in traditional Chinese medicine and has been proven in numerous animal studies. It is also well-known that Zingiberis Rhizoma (Ganjiang) is ineffective in the treatment of HF, but it can enhance the anti-HF effect of Fuzi. However, the mechanism underlying this compatibility is still not well investigated. To investigate this mechanism, a model of acute heart failure (AHF) in SD rats induced by propafenone hydrochloride was established in this study...
October 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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