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https://www.readbyqxmd.com/read/29046057/bile-duct-ligature-in-young-rats-a-revisited-animal-model-for-biliary-atresia
#1
Matias Garrido, Camila Escobar, Constanza Zamora, Carolina Rejas, Juan Varas, Mario Párraga, Sebastian San Martin, Sandra Montedónico
Biliary atresia leads to cirrhosis in the vast majority of patients and constitutes the first cause of paediatric liver transplantation. Animal models allow us to understand the molecular basis and natural history of diseases. The aim of this study is to describe a surgically created animal model of biliary atresia with emphasis in long-term liver function. Forty-two 3-week-old Sprague-Dawley rats were randomly divided into two groups: bile duct ligature (BDL) and control. The animals were sacrificed on the 2nd, 4th, and 6th postoperative weeks...
September 13, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29046050/immunofluorescent-characterization-of-non-myelinating-schwann-cells-and-their-interactions-with-immune-cells-in-mouse-mesenteric-lymph-node
#2
Zhongli Shi, Wayne K Greene, Philip K Nicholls, Dailun Hu, Janina E E Tirnitz-Parker, Qionglan Yuan, Changfu Yin, Bin Ma
The central nervous system (CNS) influences the immune system in a general fashion by regulating the systemic concentration of humoral substances, whereas the autonomic nervous system communicates specifically with the immune system according to local interactions. Data concerning the mechanisms of this bidirectional crosstalk of the peripheral nervous system (PNS) and immune system remain limited. To gain a better understanding of local interactions of the PNS and immune system, we have used immunofluorescent staining of glial fibrillary acidic protein (GFAP), coupled with confocal microscopy, to investigate the non-myelinating Schwann cell (NMSC)-immune cell interactions in mouse mesenteric lymph nodes...
August 7, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29046032/-the-research-protocol-vi-how-to-choose-the-appropriate-statistical-test-inferential-statistics
#3
Eric Flores-Ruiz, María Guadalupe Miranda-Novales, Miguel Ángel Villasís-Keever
The statistical analysis can be divided in two main components: descriptive analysis and inferential analysis. An inference is to elaborate conclusions from the tests performed with the data obtained from a sample of a population. Statistical tests are used in order to establish the probability that a conclusion obtained from a sample is applicable to the population from which it was obtained. However, choosing the appropriate statistical test in general poses a challenge for novice researchers. To choose the statistical test it is necessary to take into account three aspects: the research design, the number of measurements and the scale of measurement of the variables...
July 2017: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
https://www.readbyqxmd.com/read/29045998/-plastic-reconstructive-microsurgery-in-the-elderly-patient-consensus-statement-of-the-german-speaking-working-group-for-microsurgery-of-the-peripheral-nerves-and-vessels
#4
Ingo Ludolph, Marcus Lehnhardt, Andreas Arkudas, Ulrich Kneser, Gerhard Pierer, Yves Harder, Raymund E Horch
During the last two decades, understanding and experience of microsurgical reconstruction have increased. This is linked to improved instruments and technical devices, as well as to the increased number of available donor sites for microvascular flap transfer. If the indication is appropriate, it is now possible to perform personalised, safe and successful microsurgical reconstructions, almost independently of the patients' age. Despite these constant developments, there is little specific literature on microsurgery related to the specific problems of the elderly patient...
October 18, 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/29045978/-clinicopathologic-features-of-infant-dysembryoplastic-neuroepithelial-tumor-a-case-report-and-literature-review
#5
H Wang, J T Ye, H X Yao, D Li, Y Dong
Dysembryoplastic neuroepithelial tumor (DNT) has traditionally been viewed as rare benign tumors that present with seizure widely considered curable with surgery alone. Most DNTs occur in childhood and young adults. However, rare reported cases occur in infants. This paper reported an infant case of DNT and its diagnosis, differential diagnosis, treatment, molecular features and prognosis based on the review of current literatures. The age onset of this patient was only 11 months old. The clinical manifestations were partial seizures and the imaging data untypical; CT and MRI were all supportive of astrocytoma...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045976/-anatomy-and-histology-characteristics-of-lymph-node-in-nude-mice
#6
R Sun, B Gao, C B Guo
OBJECTIVE: To compare the differences of anatomical and histological characteristics of lymph nodes between BALB/c nude mice and BALB/c mice. METHODS: Firstly, twenty BALB/c nude mice and twenty BALB/c mice were dissected by using a surgical microscope. Secondly, the differences of T cells and B cells at the lymph node were compared by the expressions of CD3 and CD20 immunohistochemistry dyes. RESULTS: There were, on average, 23 nodes per mouse contained within the large lymph node assembly in the BALB/c nude mouse...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045957/-clinical-characteristics-of-neonatal-fungal-sepsis-in-neonatal-intensive-care-unit
#7
X Zhang, X F Ru, Y Wang, X Li, T Sang, Q Feng
OBJECTIVE: To study the characteristics of neonatal fungal sepsis and the difference between bacterial sepsis and fungal sepsis. To improve the understanding of neonatal fungal sepsis. METHODS: Clinical data of neonatal fungal sepsis in neonatal intensive care unit (NICU) were collected from 2011 to 2016 in Peking University first Hospital. The clinical characteristics were analyzed retrospectively. The difference between neonatal fungal sepsis and bacterial sepsis was also analyzed...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045954/-pulmonary-arterial-hypertension-as-leading-manifestation-of-methylmalonic-aciduria-clinical-characteristics-and-gene-testing-in-15-cases
#8
X Q Liu, H Yan, J X Qiu, C Y Zhang, J G Qi, X Zhang, H J Xiao, Y L Yang, Y H Chen, J B DU
OBJECTIVE: To deepen our understanding of Methylmalonic aciduria (MMA) associated pulmonary hypertension (PH) by analyzing the characteristics of clinical presentation, pulmonary high resolusion CT(HRCT), treatment response and gene mutation. METHODS: This study includes 15 cases of pediatric patients with MMA associated PH diagnosed and treated in Peking University First Hospital pediatric department between May 2012 and May 2016 with symptoms of PH as their leading presentation...
October 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29045919/altered-attentional-control-strategies-but-spared-executive-functioning-in-chronic-cannabis-users
#9
Amy T Nusbaum, Paul Whitney, Carrie Cuttler, Alexander Spradlin, John M Hinson, Ryan J McLaughlin
BACKGROUND: Cannabis use has increased rapidly in recent decades. The increase in cannabis use makes it important to understand the potential influence of chronic use on attentional control and other executive functions (EFs). Because cannabis is often used to reduce stress, and because stress can constrain attentional control and EFs, the primary goal of this study was to determine the joint effect of acute stress and chronic cannabis use on specific EFs. METHODS: Thirty-nine cannabis users and 40 non-users were assigned to either a stress or no stress version of the Maastricht Acute Stress Test...
October 12, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/29045913/team-based-learning-in-nursing-and-midwifery-higher-education-a-systematic-review-of-the-evidence-for-change
#10
REVIEW
Chris Dearnley, Christine Rhodes, Peter Roberts, Pam Williams, Sarah Prenton
REVIEW AIM: The aim of this study is to review the evidence in relation to the experiences and outcomes of students on nursing and/or midwifery higher education programmes, who experience team based learning. REVIEW OBJECTIVES: To examine the relationship between team based learning and attainment for nursing and midwifery students in professional higher education. To examine the relationship between team based learning and student satisfaction for nurses and midwifery students in higher education...
September 28, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29045912/the-lived-experience-of-first-year-undergraduate-student-nurses-a-hermeneutic-phenomenological-study
#11
Debra J Porteous, Alison Machin
INTRODUCTION: This study gives insight into the experiences and perceptions of one group of undergraduate nursing students as they make the transition into Higher Education and the nursing profession, during the first year, of their three-year programme. Research has shown that first year undergraduate experience is complex and challenging for any student. For undergraduate nursing students, the process of achieving additional professional practice competencies required for United Kingdom nursing registration adds additional responsibility and potentially, more pressure...
October 3, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29045892/wait-wait-%C3%A2-ok-now-go-in-inkt-cells-resolve-liver-inflammation
#12
Haiguang Wang, Kristin A Hogquist
Resolution of inflammation is pivotal to restoring tissue homeostasis, yet there is limited understanding of how this process is regulated. In this issue of Immunity, Liew et al. (2017) reveal a critical role for invariant natural killer T (iNKT) cells in switching inflammation to tissue repair in an interlukin-4-dependent process.
October 17, 2017: Immunity
https://www.readbyqxmd.com/read/29045887/polymorphism-intermolecular-interactions-and-spectroscopic-properties-in-crystal-structures-of-sulfonamides
#13
C Ignacio Sainz-Díaz, Misaela Francisco-Márquez, Catalina Soriano-Correa
The antibiotics family of sulfonamides has been used worldwide intensively in human therapeutics and farm livestock during decades. Intermolecular interactions of these sulfamides are important to understand their bioactivity and biodegradation. These interactions are also responsible for their supramolecular structures. The intermolecular interactions in the crystal polymorphs of the sulfonamides, sulfamethoxypyridazine and sulfamethoxydiazine, as models of sulfonamides, have been studied by using quantum mechanical calculations...
October 15, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29045882/untangling-the-hairball-fitness-based-asymptotic-reduction-of-biological-networks
#14
Félix Proulx-Giraldeau, Thomas J Rademaker, Paul François
Complex mathematical models of interaction networks are routinely used for prediction in systems biology. However, it is difficult to reconcile network complexities with a formal understanding of their behavior. Here, we propose a simple procedure (called ϕ¯) to reduce biological models to functional submodules, using statistical mechanics of complex systems combined with a fitness-based approach inspired by in silico evolution. The ϕ¯ algorithm works by putting parameters or combination of parameters to some asymptotic limit, while keeping (or slightly improving) the model performance, and requires parameter symmetry breaking for more complex models...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045881/confocal-rheology-probes-the-structure-and-mechanics-of-collagen-through-the-sol-gel-transition
#15
Khanh-Hoa Tran-Ba, Daniel J Lee, Jieling Zhu, Keewook Paeng, Laura J Kaufman
Fibrillar type I collagen-based hydrogels are commonly used in tissue engineering and as matrices for biophysical studies. Mechanical and structural properties of these gels are known to be governed by the conditions under which fibrillogenesis occurs, exhibiting variation as a function of protein concentration, temperature, pH, and ionic strength. Deeper understanding of how macroscopic structure affects viscoelastic properties of collagen gels over the course of fibrillogenesis provides fundamental insight into biopolymer gel properties and promises enhanced control over the properties of such gels...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045873/nanoscale-membrane-budding-induced-by-ctxb-and-detected-via-polarized-localization-microscopy
#16
Abir M Kabbani, Christopher V Kelly
For endocytosis and exocytosis, membranes transition among planar, budding, and vesicular topographies through nanoscale reorganization of lipids, proteins, and carbohydrates. However, prior attempts to understand the initial stages of nanoscale bending have been limited by experimental resolution. Through the implementation of polarized localization microscopy, this article reports the inherent membrane bending capability of cholera toxin subunit B (CTxB) in quasi-one-component-supported lipid bilayers. Membrane buds were first detected with <50 nm radius, grew to >200 nm radius, and extended into longer tubules with dependence on the membrane tension and CTxB concentration...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045868/identification-and-characterization-of-an-inside-out-folding-intermediate-of-t4-phage-sliding-clamp
#17
Manika Indrajit Singh, Vikas Jain
Protein folding process involves formation of transiently occurring intermediates that are difficult to isolate and characterize. It is both necessary and interesting to characterize the structural conformations adopted by these intermediates, also called molten globules (MG), to understand protein folding. Here, we investigated the equilibrium (un)folding intermediate state of T4 phage gene product 45 (gp45, also known as DNA polymerase processivity factor or sliding clamp) obtained during chemical denaturation...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045867/enzyme-triggered-dissociation-of-a-fret-based-protein-biosensor-monitored-by-synchrotron-saxs
#18
Greta Faccio, Stefan Salentinig
Protein biosensors are widely used for the monitoring of metabolite concentration and enzymatic activities inside living cells and in in vitro applications. Neutrophil elastase (NE) is a serine protease of relevance in inflammatory diseases whose activity can lead to pathological conditions if unregulated. This study focuses on the structural characterization of a biosensor for NE activity based on Förster resonance energy transfer (FRET). The cleavage by NE results in dissociation of the FRET fluorescent protein pair and alteration of the fluorescent emission spectrum...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045866/how-many-protein-sequences-fold-to-a-given-structure-a-coevolutionary-analysis
#19
Pengfei Tian, Robert B Best
Quantifying the relationship between protein sequence and structure is key to understanding the protein universe. A fundamental measure of this relationship is the total number of amino acid sequences that can fold to a target protein structure, known as the "sequence capacity," which has been suggested as a proxy for how designable a given protein fold is. Although sequence capacity has been extensively studied using lattice models and theory, numerical estimates for real protein structures are currently lacking...
October 17, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29045862/the-two-state-prehensile-tail-of-the-antibacterial-toxin-colicin-n
#20
Christopher L Johnson, Alexandra S Solovyova, Olli Hecht, Colin Macdonald, Helen Waller, J Günter Grossmann, Geoffrey R Moore, Jeremy H Lakey
Intrinsically disordered regions within proteins are critical elements in many biomolecular interactions and signaling pathways. Antibacterial toxins of the colicin family, which could provide new antibiotic functions against resistant bacteria, contain disordered N-terminal translocation domains (T-domains) that are essential for receptor binding and the penetration of the Escherichia coli outer membrane. Here we investigate the conformational behavior of the T-domain of colicin N (ColN-T) to understand why such domains are widespread in toxins that target Gram-negative bacteria...
October 17, 2017: Biophysical Journal
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