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https://www.readbyqxmd.com/read/27500655/music-chills-the-eye-pupil-as-a-mirror-to-music-s-soul
#1
Bruno Laeng, Lise Mette Eidet, Unni Sulutvedt, Jaak Panksepp
This study evaluated whether music-induced aesthetic "chill" responses, which typically correspond to peak emotional experiences, can be objectively monitored by degree of pupillary dilation. Participants listened to self-chosen songs versus control songs chosen by other participants. The experiment included an active condition where participants made key presses to indicate when experiencing chills and a passive condition (without key presses). Chills were reported more frequently for self-selected songs than control songs...
August 2016: Consciousness and Cognition
https://www.readbyqxmd.com/read/27329312/nucleus-morphometry-in-cultured-epithelial-cells-correlates-with-phenotype
#2
Ayyad Z Khan, Tor P Utheim, Catherine J Jackson, Sjur Reppe, Torstein Lyberg, Jon R Eidet
Phenotype of cultured ocular epithelial transplants has been shown to affect clinical success rates following transplantation to the cornea. The purpose of this study was to evaluate the relationship between cell nucleus morphometry and phenotype in three types of cultured epithelial cells. This study provides knowledge for the development of a non-invasive method of determining the phenotype of cultured epithelium before transplantation. Cultured human conjunctival epithelial cells (HCjE), human epidermal keratinocytes (HEK), and human retinal pigment epithelial cells (HRPE) were analyzed by quantitative immunofluorescence...
June 2016: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/27259952/impact-of-storage-temperature-on-the-expression-of-cell-survival-genes-in-cultured-arpe-19-cells
#3
Lara Pasovic, Jon R Eidet, Ole K Olstad, Dong F Chen, Torstein Lyberg, Tor P Utheim
PURPOSE: The development of a suitable storage method for retinal pigment epithelium (RPE) is necessary in the establishment of future RPE replacement therapy, and storage temperature has proven to be pivotal for cell survival. ARPE-19, a widely used model for RPE, has been shown to yield the greatest number of viable cells when stored at 16°C compared to other storage temperatures. In this study, we analyze the gene expression profile of cultured ARPE-19 cells after seven days of storage at different temperatures in an effort to predict the gene-level consequences of storage of RPE transplants...
June 3, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27241050/long-term-outcomes-after-transcatheter-aortic-valve-implantation-the-impact-of-intraoperative-tissue-doppler-echocardiography
#4
Jo Eidet, Gry Dahle, Jan F Bugge, Bjørn Bendz, Kjell A Rein, Lars Aaberge, Jon T Offstad, Erik Fosse, Svend Aakhus, Per S Halvorsen
OBJECTIVES: Transcatheter aortic valve implantation improves survival in patients with severe aortic stenosis who are ineligible for surgical valve replacement; however, not all patients benefit from the procedure. We endeavoured to identify these patients using intraoperative echocardiography and hypothesized that intraoperative left ventricular function in response to the acute afterload reduction during the procedure was related to long-term outcomes. METHODS: We prospectively included 64 patients who were scheduled for transcatheter aortic valve implantation and divided them into responders and non-responders based on their left ventricular intraoperative responses to the acute afterload reduction after valve deployment...
September 2016: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/27148875/dry-eye-disease-patients-with-xerostomia-report-higher-symptom-load-and-have-poorer-meibum-expressibility
#5
Ida G Fostad, Jon R Eidet, Tor P Utheim, Sten Ræder, Neil S Lagali, Edvard B Messelt, Darlene A Dartt
The purpose of the study was to investigate if xerostomia (dry mouth) is associated with symptoms and signs of dry eye disease (DED). At the Norwegian Dry Eye Clinic, patients with symptomatic DED with different etiologies were consecutively included in the study. The patients underwent a comprehensive ophthalmological work-up and completed self-questionnaires on symptoms of ocular dryness (Ocular Surface Disease Index [OSDI] and McMonnies Dry Eye Questionnaire) and the Sjögren's syndrome (SS) questionnaire (SSQ)...
2016: PloS One
https://www.readbyqxmd.com/read/27023475/storage-temperature-alters-the-expression-of-differentiation-related-genes-in-cultured-oral-keratinocytes
#6
Tor Paaske Utheim, Rakibul Islam, Ida G Fostad, Jon R Eidet, Amer Sehic, Ole K Olstad, Darlene A Dartt, Edward B Messelt, May Griffith, Lara Pasovic
PURPOSE: Storage of cultured human oral keratinocytes (HOK) allows for transportation of cultured transplants to eye clinics worldwide. In a previous study, one-week storage of cultured HOK was found to be superior with regard to viability and morphology at 12°C compared to 4°C and 37°C. To understand more of how storage temperature affects cell phenotype, gene expression of HOK before and after storage at 4°C, 12°C, and 37°C was assessed. MATERIALS AND METHODS: Cultured HOK were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium at 4°C, 12°C, and 37°C for one week...
2016: PloS One
https://www.readbyqxmd.com/read/26940175/the-silk-protein-sericin-induces-rapid-melanization-of-cultured-primary-human-retinal-pigment-epithelial-cells-by-activating-the-nf-%C3%AE%C2%BAb-pathway
#7
J R Eidet, S Reppe, L Pasovic, O K Olstad, T Lyberg, A Z Khan, I G Fostad, D F Chen, T P Utheim
Restoration of the retinal pigment epithelial (RPE) cells to prevent further loss of vision in patients with age-related macular degeneration represents a promising novel treatment modality. Development of RPE transplants, however, requires up to 3 months of cell differentiation. We explored whether the silk protein sericin can induce maturation of primary human retinal pigment epithelial (hRPE) cells. Microarray analysis demonstrated that sericin up-regulated RPE-associated transcripts (RPE65 and CRALBP). Upstream analysis identified the NF-κB pathway as one of the top sericin-induced regulators...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26857433/transpulmonary-thermodilution-picco-measurements-in-children-without-cardiopulmonary-dysfunction-large-interindividual-variation-and-conflicting-reference-values
#8
Guro Grindheim, Jo Eidet, Gunnar Bentsen
BACKGROUND: The PiCCO system, based on transpulmonary thermodilution, is one of the few tools available for continuous hemodynamic monitoring in children. However, published data for some of the derived variables reveal indexed values that seem questionable. AIMS: The aim of this study was to collect data from hemodynamically normal children and compare these to existing reference values. Furthermore, we sought to explore if indexing some of the variables differently could improve the clinical application of the obtained values...
April 2016: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/26690486/concise-review-comparison-of-culture-membranes-used-for-tissue-engineered-conjunctival-epithelial-equivalents
#9
REVIEW
Jon Roger Eidet, Darlene A Dartt, Tor Paaske Utheim
The conjunctival epithelium plays an important role in ensuring the optical clarity of the cornea by providing lubrication to maintain a smooth, refractive surface, by producing mucins critical for tear film stability and by protecting against mechanical stress and infectious agents. A large number of disorders can lead to scarring of the conjunctiva through chronic conjunctival inflammation. For controlling complications of conjunctival scarring, surgery can be considered. Surgical treatment of symblepharon includes removal of the scar tissue to reestablish the deep fornix...
December 11, 2015: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/26467639/intraoperative-improvement-in-left-ventricular-peak-systolic-velocity-predicts-better-short-term-outcome-after-transcatheter-aortic-valve-implantation
#10
Jo Eidet, Gry Dahle, Jan Frederik Bugge, Bjørn Bendz, Kjell Arne Rein, Lars Aaberge, Jon Thomas Offstad, Erik Fosse, Svend Aakhus, Per Steinar Halvorsen
OBJECTIVES: Left ventricular function is expected to improve after transcatheter aortic valve implantation due to the acute reduction in afterload, but does not occur in all patients. We hypothesized that the immediate intraoperative response in systolic left ventricular longitudinal motion during the procedure could be a predictor of short-term outcome. METHODS: Sixty-four patients treated with transcatheter aortic valve implantation for severe aortic stenosis were included...
January 2016: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/26448872/effect-of-storage-temperature-on-key-functions-of-cultured-retinal-pigment-epithelial-cells
#11
Lara Pasovic, Jon Roger Eidet, Berit S Brusletto, Torstein Lyberg, Tor P Utheim
Purpose. Replacement of the diseased retinal pigment epithelium (RPE) with cells capable of performing the specialized functions of the RPE is the aim of cell replacement therapy for treatment of macular degenerative diseases. A storage method for RPE is likely to become a prerequisite for the establishment of such treatment. Herein, we analyze the effect of storage temperature on key functions of cultured RPE cells. Methods. Cultured ARPE-19 cells were stored in Minimum Essential Medium at 4°C, 16°C, and 37°C for seven days...
2015: Journal of Ophthalmology
https://www.readbyqxmd.com/read/26398483/effect-of-storage-temperature-on-the-phenotype-of-cultured-epidermal-cells-stored-in-xenobiotic-free-medium
#12
Catherine Jackson, Jon R Eidet, Sjur Reppe, Hans Christian D Aass, Kim A Tønseth, Borghild Roald, Torstein Lyberg, Tor P Utheim
PURPOSE: Cultured epidermal cell sheets (CECS) are used in the treatment of large area burns to the body and have potential to treat limbal stem cell deficiency (LSCD) as shown in animal studies. Despite widespread use, storage options for CECS are limited. Short-term storage allows flexibility in scheduling surgery, quality control and improved transportation to clinics worldwide. Recent evidence points to the phenotype of cultured epithelial cells as a critical predictor of post-operative success following transplantation of CECS in burns and in transplantation of cultured epithelial cells in patients with LSCD...
June 2016: Current Eye Research
https://www.readbyqxmd.com/read/26052937/effect-of-storage-temperature-on-structure-and-function-of-cultured-human-oral-keratinocytes
#13
Rakibul Islam, Catherine Jackson, Jon R Eidet, Edward B Messelt, Rima Maria Corraya, Torstein Lyberg, May Griffith, Darlene A Dartt, Tor P Utheim
PURPOSE/AIMS: To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). MATERIALS AND METHODS: Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4 °C increments from 4 °C to 37 °C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy...
2015: PloS One
https://www.readbyqxmd.com/read/25734654/serum-free-and-xenobiotic-free-preservation-of-cultured-human-limbal-epithelial-cells
#14
Oeygunn Utheim, Rakibul Islam, Torstein Lyberg, Borghild Roald, Jon Roger Eidet, Maria Fideliz de la Paz, Darlene A Dartt, Sten Raeder, Tor Paaske Utheim
AIM/PURPOSE OF THE STUDY: To develop a one-week storage method, without serum and xenobiotics, that would maintain cell viability, morphology, and phenotype of cultured human limbal epithelial sheets. MATERIALS AND METHODS: Human limbal explants were cultured on intact human amniotic membranes for two weeks. The sheets were stored in a hermetically sealed container at 23°C in either a serum-free medium with selected animal serum-derived compounds (Quantum 286) or a xenobiotic-free medium (Minimal Essential Medium) for 4 and 7 days...
2015: PloS One
https://www.readbyqxmd.com/read/25658020/xenobiotic-and-serum-free-culture-of-oral-mucosal-epithelial-cells-on-contact-lenses
#15
Benny Björkblom, Jon R Eidet, Tor P Utheim, Harald F Ulltveit-Moe, Sten Raeder
PURPOSE/AIM: Cultured autologous oral mucosal epithelial cells (OMECs) have proven useful in the treatment of ocular surface disorders. This study is the first to investigate the potential of expanding OMEC in a xenobiotic- and serum-free medium using therapeutic contact lenses (CLs) as a substrate and carrier. MATERIALS AND METHODS: Porcine OMEC were seeded on laminin-coated lotrafilcon A therapeutic CLs with the density of 8 × 10(4) cells/lens and cultured in a defined serum and xenobiotic-free medium...
2016: Current Eye Research
https://www.readbyqxmd.com/read/25620143/transcatheter-aortic-valve-implantation-and-intraoperative-left-ventricular-function-a-myocardial-tissue-doppler-imaging-study
#16
Jo Eidet, Gry Dahle, Jan F Bugge, Bjørn Bendz, Kjell A Rein, Erik Fosse, Svend Aakhus, Per S Halvorsen
OBJECTIVE: Transcatheter aortic valve implantation in patients turned down for surgical aortic valve replacement is a high-risk procedure. Severe aortic stenosis is associated with impaired left ventricular longitudinal motion, and myocardial peak systolic velocity is a measure of left ventricular function in these patients. The present study aimed to quantify the acute changes in left ventricular function during the procedure by using myocardial tissue Doppler imaging and transthoracic cardiac output measurements...
February 2015: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/25302580/reliability-of-corneal-dynamic-scheimpflug-analyser-measurements-in-virgin-and-post-prk-eyes
#17
Xiangjun Chen, Aleksandar Stojanovic, Yanjun Hua, Jon Roger Eidet, Di Hu, Jingting Wang, Tor Paaske Utheim
PURPOSE: To determine the measurement reliability of CorVis ST, a dynamic Scheimpflug analyser, in virgin and post-photorefractive keratectomy (PRK) eyes and compare the results between these two groups. METHODS: Forty virgin eyes and 42 post-PRK eyes underwent CorVis ST measurements performed by two technicians. Repeatability was evaluated by comparing three consecutive measurements by technician A. Reproducibility was determined by comparing the first measurement by technician A with one performed by technician B...
2014: PloS One
https://www.readbyqxmd.com/read/25170754/effect-of-storage-temperature-on-cultured-epidermal-cell-sheets-stored-in-xenobiotic-free-medium
#18
Catherine Jackson, Peder Aabel, Jon R Eidet, Edward B Messelt, Torstein Lyberg, Magnus von Unge, Tor P Utheim
Cultured epidermal cell sheets (CECS) are used in regenerative medicine in patients with burns, and have potential to treat limbal stem cell deficiency (LSCD), as demonstrated in animal models. Despite widespread use, short-term storage options for CECS are limited. Advantages of storage include: flexibility in scheduling surgery, reserve sheets for repeat operations, more opportunity for quality control, and improved transportation to allow wider distribution. Studies on storage of CECS have thus far focused on cryopreservation, whereas refrigeration is a convenient method commonly used for whole skin graft storage in burns clinics...
2014: PloS One
https://www.readbyqxmd.com/read/25134496/antioxidants-improve-the-viability-of-stored-adult-retinal-pigment-epithelial-19-cultures
#19
Lara Pasovic, Jon R Eidet, Torstein Lyberg, Edward B Messelt, Peder Aabel, Tor P Utheim
INTRODUCTION: There is increasing evidence that retinal pigment epithelium (RPE) can be used to treat age-related macular degeneration, one of the leading causes of blindness worldwide. However, the best way to store RPE to enable worldwide distribution is unknown. We investigated the effects of supplementing our previously published storage method with seven additives, attempting to improve the number of viable adult retinal pigment epithelial (ARPE)-19 cells after storage. MATERIALS AND METHODS: ARPE-19 cells were cultured on multiwell plates before being stored for 1 week at 16 °C...
December 2014: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/24971457/effect-of-time-delay-on-recognition-memory-for-pictures-the-modulatory-role-of-emotion
#20
Bo Wang
This study investigated the modulatory role of emotion in the effect of time delay on recognition memory for pictures. Participants viewed neutral, positive and negative pictures, and took a recognition memory test 5 minutes, 24 hours, or 1 week after learning. The findings are: 1) For neutral, positive and negative pictures, overall recognition accuracy in the 5-min delay did not significantly differ from that in the 24-h delay. For neutral and positive pictures, overall recognition accuracy in the 1-week delay was lower than in the 24-h delay; for negative pictures, overall recognition in the 24-h and 1-week delay did not significantly differ...
2014: PloS One
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