Rakibul Islam, Rima Maria Corraya, Lara Pasovic, Ayyad Zartasht Khan, Hans Christian D Aass, Jon Roger Eidet, Tor Paaske Utheim
This study aimed to investigate how prolonged storage of adult retinal pigment epithelial (ARPE-19) cell sheets affects cell metabolism, morphology, viability, and phenotype. ARPE-19 cell sheets were stored at three temperatures (4 °C, 16 °C, and 37 °C) for three weeks. Metabolic status and morphology of the cells were monitored by sampling medium and examining cells by phase-contrast microscopy, respectively, throughout the storage period. Cell viability was analyzed by flow cytometry, and phenotype was determined by epifluorescence microscopy after the storage...
December 9, 2020: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry