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aseptic sterilization

Vijita Vijay Nair, G N Karibasappa, Arun Dodamani, V K Prashanth
BACKGROUND: Loss of teeth affects the individual's health. Many factors determine the need to wear a removable dental prosthesis. Due to nature of design, age and lack of awareness, prosthesis often are neglected to maintain in an aseptic condition leading to microbial contamination. This provided an impetus for the present study with the aim of determining the microbial contamination of removable dental prosthesis. METHODOLOGY: Total, 45 patients wearing removable dental prosthesis were randomly selected...
October 2016: Journal of Indian Prosthodontic Society
A Miglio, V Stefanetti, M T Antognoni, K Cappelli, S Capomaccio, M Coletti, F Passamonti
BACKGROUND: Bacterial contamination of whole blood (WB) units can result in transfusion-transmitted infection, but the extent of the risk has not been established and may be underestimated in veterinary medicine. OBJECTIVES: To detect, quantify, and identify bacterial microorganisms in 49 canine WB units during their shelf life. ANIMALS: Forty-nine healthy adult dogs. METHODS: Forty-nine WB units were included in the study...
October 13, 2016: Journal of Veterinary Internal Medicine
Jenny X Chen, Blake C Alkire, Allen C Lam, William T Curry, Eric H Holbrook
Objectives While bacterial meningitis is a concerning complication after endoscopic skull base surgery, the diagnosis can be made without consideration for aseptic meningitis. This article aims to (1) present a patient with recurrent craniopharyngioma and multiple postoperative episodes of aseptic meningitis and (2) discuss the diagnosis and management of aseptic meningitis. Design Case report and literature review. Results A 65-year-old female patient with a symptomatic craniopharyngioma underwent transsphenoidal resection...
October 2016: Journal of Neurological Surgery Reports
Todd J Nilsen, Anouska Dasgupta, Yen-Chen Huang, Henry Wilson, Evangelia Chnari
BACKGROUND: The use of acellular dermal matrices (ADMs) has become the standard of practice in many reconstructive and aesthetic surgical applications. Different methods used to prepare the allograft tissue for surgical use can alter the ADMs natural properties. Aseptic processing has been shown to retain the natural properties of ADMs more favorably than terminally sterilized ADMs. Terminal sterilization has been historically linked to alteration of biological materials. In vitro work was conducted to compare ADM processing methods...
October 3, 2016: Aesthetic Surgery Journal
Christopher M Aguila, Gaëtan J-R Delcroix, David N Kaimrajh, Edward L Milne, H Thomas Temple, Loren L Latta
PURPOSE: This study was designed to investigate the biomechanical properties of nonirradiated (NI) and irradiated (IR) peroneus tendons to determine if they would be suitable allografts, in regards to biomechanical properties, for anterior cruciate ligament reconstruction after a dose of 1.5-2.5 Mrad. METHODS: Seven pairs of peroneus longus (PL) and ten pairs of peroneus brevis (PB) tendons were procured from human cadavers. The diameter of each allograft was measured...
2016: Open Access Journal of Sports Medicine
Sung Hye Byun, So Young Lee, Seong Yeon Hong, Taeha Ryu, Baek Jin Kim, Jin Yong Jung
INTRODUCTION: In fetuses who are predicted to be at risk of catastrophic airway obstruction at delivery, the ex utero intrapartum treatment (EXIT) procedure is useful for securing the fetal airway while maintaining fetal oxygenation via placental circulation. Factors, including poor posture of the fetus and physician, narrow visual field, and issues of contamination in the aseptic surgical field, make fetal intubation during the EXIT procedure difficult. Herein, we report our experience of the usefulness of the GlideScope video laryngoscope (GVL) for intubation during the EXIT procedure...
September 2016: Medicine (Baltimore)
Rakesh Hasabe, Kumud Gupta, Pallavi Rathode
INTRODUCTION: Conventionally postpartum hemorrhage (PPH) has been defined as blood loss of more than 500 ml following vaginal delivery and 1000 ml following a cesarean section [Pritchard et al. in Am J Obstet Gynecol 84(10):1271-1282, (1962)]. Another definition labels PPH as any blood loss which causes a 10 % drop in hematocrit [Combs et al. in Obstet Gynecol 77:69-76, (1991)] or which threatens the hemodynamic stability of the patient and necessitates blood transfusion [Prendiville et al...
October 2016: Journal of Obstetrics and Gynaecology of India
Guinet Marie Frédéric Roland, Berthoumieu Nicole, Dutot Philippe, Triquet Julien, Ratajczak Mehdi, Thibaudon Michel, Bechaud Philippe, Arliaud Christophe, Miclet Edith, Giordano Florine, Larcon Marjorie, Arthaud Catherine
Environmental monitoring and aseptic process simulations represent an integral part of the microbiological quality control system of sterile pharmaceutical products manufacturing operations. However, guidance documents and manufacturers practices differ regarding recommendation for incubation time and incubation temperature and, consequently, the environmental monitoring and aseptic process simulation incubation strategy should be supported by validation data. To avoid any bias coming from in vitro studies or from single manufacturing in situ site studies, we performed a collaborative study at four manufacturing sites with four samples at the same location...
September 4, 2016: PDA Journal of Pharmaceutical Science and Technology
Martha F Mushi, Gustave Buname, Oliver Bader, Uwe Groß, Stephen E Mshana
BACKGROUND: Suppurative otitis media (SOM) is a major public health concern worldwide and is associated with increased morbidity. Cases of fungal suppurative otitis media were studied to establish the effect of fungi in otitis media. METHODS: Ear swabs from 410 patients were collected aseptically using sterile cotton swabs from discharging ear through perforated tympanic membrane. Swabs were subjected to microscopic and culture investigations. The species of fungal growing on Sabouraud's agar were identified using MALDI-TOF MS...
2016: BMC Infectious Diseases
Tihtina Kibret, Adane Bitew
BACKGROUND: Fungal keratitis is an important cause of corneal blindness all over the world. Although there are several reports on fungal keratitis from developing and developed countries, fungal keratitis in Ethiopia is poorly known. The aim of this study was to determine the prevalence of fungal keratitis and spectrum of fungi implicated in causing the infection. METHODS: The present study was a single institutional cross-sectional study carried out in Minilik II Memorial Hospital eye clinic, Addis Ababa, Ethiopia from September 2014 to August 2015...
August 30, 2016: BMC Ophthalmology
Daniel A Lyons, Shaun D Mendenhall, Michael W Neumeister, Paul S Cederna, Adeyiza O Momoh
BACKGROUND: As the use of acellular dermal matrices in breast reconstruction has become more commonplace and efforts are made to improve on postoperative outcomes, the method of acellular dermal matrix (ADM) processing (aseptic versus sterile) has become a subject of interest. This article provides an updated overview of the critical aspects of ADM processing in addition to application of ADMs in single- and two-stage breast reconstruction, a review of the morbidity associated with ADM use, and alternatives...
July 2016: Plastic and Reconstructive Surgery. Global Open
Ludovit Gaspar, Michal Reich, Zoltan Kassai, Fedor Macasek, Luis Rodrigo, Peter Kruzliak, Peter Kovac
Nine years of experience with 2286 commercial synthesis allowed us to deliver comprehensive information on the quality of (18)F-FDG production. Semi-automated FDG production line using Cyclone 18/9 machine (IBA Belgium), TRACERLab MXFDG synthesiser (GE Health, USA) using alkalic hydrolysis, grade "A" isolator with dispensing robotic unit (Tema Sinergie, Italy), and automatic control system under GAMP5 (minus2, Slovakia) was assessed by TQM tools as highly reliable aseptic production line, fully compliant with Good Manufacturing Practice and just-in-time delivery of FDG radiopharmaceutical...
2016: American Journal of Nuclear Medicine and Molecular Imaging
M D Wimmer, M M Ploeger, M J Friedrich, R Bornemann, P P Roessler, S Gravius, T M Randau
BACKGROUND: When deciding upon the best treatment strategy in revision arthroplasty, it is absolutely crucial to use the best possible preoperative detection whether a periprosthetic joint infection (PJI) is present or not. New molecular markers investigated in serum samples and synovial fluid can help to improve the preoperative diagnosis. In 2001, a novel IL-6 lateral flow immunoassay testing device was introduced which has never been tested in synovial fluid so far. OBJECTIVE: For our study we investigated whether the test can be used safely, feasibly and time effectively with synovial fluid gathered from potentially infected THAs or TKAs and whether the already published cutoff for IL-6 in synovial fluid predicting a PJI can be reproduced using the QuickLine IL-6 immunoassay...
July 29, 2016: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
Sandra L Smith, Laura Serke
Mother's milk is the recommended food for premature infants cared for in the NICU. In the cases presented in this article, mothers pumped their milk into food-grade aseptic plastic containers. Milk was refrigerated before use. In Case 1, an infant developed Pseudomonas aeruginosa sepsis. In Case 2, an infant developed methicillin-resistant Staphylococcus aureus. Both cases were attributed to contaminated mother's milk. Proper cleaning and sterilization of pump parts is essential to prevent milk contamination...
September 2016: Journal of Obstetric, Gynecologic, and Neonatal Nursing: JOGNN
I Shrestha, M Pokharel, A Dhakal, R Cm Amatya, S Madhup, J B Sherchan
Background Nasal packs are utilized nearly by otorhinolaryngologists for controlling epistaxis and post nasal procedures. Complications have been reported due to them; therefore the use of antibiotics is a common practice among otorhinolaryngologists. Objective To detect microbiological flora associated with nasal packing and find evidence to support the benefit of systemic antibiotics with it. Method A prospective, analytical study was conducted on 51 patients presenting to the Department of ENT, KUSMS from June to September 2015 who required nasal packing...
October 2015: Kathmandu University Medical Journal (KUMJ)
Bhushan S Bhadane, Ravindra H Patil
Surface sterilization of explant is an important and most sensitive step in plant tissue culture. Inappropriate concentrations of sterilants have lethal effect in cell division and it restricts growth and development of explant. Therefore, suitable concentration, combinations and duration of exposure of sterilant is essential to raise in vitro cultures successfully. This data demonstrates use of various sterilizing agents for aseptic plantlet germination from seed of Carissa carandas (Apocynaceae). The present dataset provides information in support of cost-effective explant sterilization potential of benzalkonium chloride containing commercial bleach (Lizol) and its comparison with traditionally used surface sterilants in plant tissue culture i...
June 2016: Data in Brief
M Guembe, M J Pérez-Granda, R Cruces, L Alcalá, E Bouza
Catheter connectors used in hemodialysis patients are those with open caps to manage high blood flows. However, current guidelines for the prevention of catheter infections recommend closed connectors. Tego™ is a closed connector designed to enable high blood flows. We used an in vitro model to compare the efficacy of Tego™ against contamination with that of standard caps in a real-life practice scenario. The model consisted of 200 blood culture bottles (BCB) with an inserted cannula closed either with Tego™ (100) or with open caps (100)...
August 2016: European Journal of Clinical Microbiology & Infectious Diseases
Anna Margareta Wagner, Werner Krenger, Shamsiya Stettler, Elke Beutler, Judith Herbst, Daniel V Surbek, Gabriela M Baerlocher
BACKGROUND: With increasing demand for umbilical cord blood units (CBUs) with total nucleated cell (TNC) counts of more than 150 × 10(7) , preshipping assessment is mandatory. Umbilical cord blood processing requires aseptic techniques and laboratories with specific air quality and cleanliness. Our aim was to establish a fast and efficient method for determining TNC counts at the obstetric ward without exposing the CBU to the environment. STUDY DESIGN AND METHODS: Data from a total of 151 cord blood donations at a single procurement site were included in this prospective study...
May 17, 2016: Transfusion
Evelina Tacconelli, Niklas F Müller, Sebastian Lemmen, Nico T Mutters, Stefan Hagel, Elisabeth Meyer
BACKGROUND: The main objective of hospital hygiene and infection prevention is to protect patients from preventable nosocomial infections. It was recently stated that the proper goal should be for zero infection rates in sterile surgical procedures. In this article, we attempt to determine whether this demand is supported by the available literature. METHODS: We systematically searched the Medline and EMBASE databases for studies published in the last 10 years on the efficacy of infection control measures and carried out a meta-analysis according to the PRISMA tool...
April 22, 2016: Deutsches Ärzteblatt International
Elaine Daniell, Trabue Bryans, Kimbrell Darnell, Joyce Hansen, Victoria M Hitchins, Manuel Saavedra
The applications for sterility testing in the validation and routine control of sterilization of medical devices have changed dramatically over the years. As the definition of sterility assurance has evolved, so has the state of the science associated with product sterility testing. Historically, product sterility testing has been applied to such things as sterilization validation, sterilization lot release, packaging qualification, aseptic processing qualification, and determination of shelf life for the packaged medical device...
April 2, 2016: Biomedical Instrumentation & Technology
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