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aseptic sterilization

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https://www.readbyqxmd.com/read/29311196/non-touch-aseptic-technique-maintains-sterility-of-antibiotic-admixed-peritoneal-dialysis-fluid
#1
Louis L Huang, Ellen Ramas, Priti Prasad, Jenny Catania, Pauline Meade, Eamonn Butler, Lawrence P McMahon
There is a paucity of data on the sterility of peritoneal dialysis fluid (PDF) after drug admixture. International Society for Peritoneal Dialysis (ISPD) guidelines suggest using sterile technique when admixing antibiotics; however, the degree of sterility remains unclear. This issue is most pertinent when preparing take-home PDF for outpatient treatment of peritonitis. This study compares the sterility of PDF admixed with antibiotics using a non-touch aseptic technique (NTAT) versus sterile technique.Groups of 8 PDF mixtures (1...
January 2018: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/29295779/a-survey-of-international-antisepsis-procedures-for-neuraxial-catheterisation-in-labour
#2
K Fayman, A Allan, C Hudson, M Logarta
BACKGROUND: Neuraxial analgesia during labour is a mainstay of anaesthetic practice globally. Despite the potential for significant neurological and infectious complications, international antisepsis practices for neuraxial anaesthesia vary widely. AIMS: The primary aim of this study was to clarify international antisepsis practices prior to neuraxial analgesia in labour. The secondary aim was to determine an approximate international incidence of neuraxial infections and neurological complications secondary to neuraxial analgesia techniques in labour...
October 6, 2017: International Journal of Obstetric Anesthesia
https://www.readbyqxmd.com/read/29284355/stability-of-sodium-bicarbonate-injection-8-4-in-syringes-over-a-six-week-period-in-refrigerated-temperature
#3
Jack T Seki, Tian Q Wang, Paul M Yip, Tony Mazzulli, Mark D Minden
Background Dysfunctional central venous catheter prohibits the administration of potential life-saving chemotherapy and the delivery of essential supportive care needs to patients. Sodium bicarbonate injection has been shown to impede against fibrin clot formation and prolong prothrombin time and thrombin clotting time. Sodium bicarbonate injection has been tried as a second-line agent with good results in a small number of patients (internal data not published) when alteplase failed. We assessed whether the pre-filled sodium bicarbonate injection in 5 mL syringes would not only preserve sterility and retain its pH and concentration but also amount to the potential cost savings for future use when stored in a refrigerated environment...
January 1, 2017: Journal of Oncology Pharmacy Practice
https://www.readbyqxmd.com/read/29276291/extended-stability-of-epinephrine-hydrochloride-injection-in-polyvinyl-chloride-bags-stored-in-amber-ultraviolet-light-blocking-bags
#4
Edward T Van Matre, Kang C Ho, Clark Lyda, Beth A Fullmer, Alan R Oldland, Tyree H Kiser
Objective: The objective of this study was to evaluate the stability of epinephrine hydrochloride in 0.9% sodium chloride in polyvinyl chloride bags for up to 60 days. Methods: Dilutions of epinephrine hydrochloride to concentrations of 16 and 64 µg/mL were performed under aseptic conditions. The bags were then placed into ultraviolet light-blocking bags and stored at room temperature (23°C-25°C) or under refrigeration (3°C-5°C). Three samples of each preparation and storage environment were analyzed on days 0, 30, 45, and 60...
September 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/29219196/evaluation-of-efficacy-of-repeated-decontamination-and-sterilization-of-single-incision-laparoscopic-surgery-ports-intended-for-1-time-use
#5
Brian Petrovsky, Eric Monnet
OBJECTIVE: To determine the efficacy of repeated decontamination and sterilization of a disposable port intended for 1-time use during single-incision laparoscopy. STUDY DESIGN: Experimental; prospective, controlled design. METHODS: Six single-access ports used 4 times and 6 single-access ports used 8 times to perform various clean, minimally invasive surgeries were evaluated. Ports were decontaminated in an enzymatic cleaner (dilution, 3:100) and cleaned with a scrub brush for 5 minutes...
December 8, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29212102/-conservative-treatment-of-an-aseptic-abscess-syndrome-with-splenic-abscesses-in-crohn-s-disease
#6
Thomas Göbel, Jutta Rauen-Vossloh, Hubert Georg Hotz, Andree Boldt, Andreas Erhardt
A 24-year old woman with a history of Crohn's disease developed bloody diarrhea and multiple abdominal abscesses, daily fever, leukocytosis, and elevated CRP several months after her immunosuppressive therapy with azathioprine was stopped. Recurrent abscess punctures did not detect any pathogenic germs and neither clinical nor serological response was achieved by administration of different antimicrobial therapies. Additionally, new splenic abscesses arose despite ongoing therapy. Under the suspicion of the rare aseptic abscess syndrome, representing an auto-inflammatory, extra-intestinal manifestation of Crohn's disease, the antimicrobial therapy was stopped and an intravenous therapy with prednisolone was initiated...
December 2017: Zeitschrift Für Gastroenterologie
https://www.readbyqxmd.com/read/29204021/cranioplasty-using-autoclaved-autologous-skull-bone-flaps-preserved-at-ambient-temperature
#7
Dominic Anto, Raju Paul Manjooran, Rajeev Aravindakshan, Kumar Lakshman, Raymond Morris
Context: Decompressive craniectomy followed by cranioplasty (CP) uses autologous craniectomy flaps or synthetic materials like titanium. Sterilization and preservation methods for the autologous bone flaps continue to be the surgeon's choice. Aim: This study aimed to assess the short-term as well as long-term clinical outcomes of CP using autoclaved autologous bone grafts. Settings and Design: This retrospective observational study was performed on patients admitted in a tertiary care teaching neurosurgery department...
October 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/29175537/vitrification-of-human-pronuclear-oocytes-by-direct-plunging-into-cooling-agent-non-sterile-liquid-nitrogen-vs-sterile-liquid-air
#8
Vladimir Isachenko, Plamen Todorov, Akerke Seisenbayeva, Yerzhan Toishibekov, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Dolores Foth, Markus Merzenich
In fact, a full sterilization of commercially-produced liquid nitrogen contaminated with different pathogens is not possible. The aim of this study was to compare the viability of human pronuclear oocytes subjected to cooling by direct submerging of open carrier in liquid nitrogen versus submerging in clean liquid air (aseptic system). One- and three-pronuclei stage embryos (n = 444) were cryopreserved by direct plunging into liquid nitrogen (vitrified) in ethylene glycol (15%), dimethylsulphoxide (15%) and 0...
November 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/29169935/survey-to-explore-understanding-of-the-principles-of-aseptic-technique-qualitative-content-analysis-with-descriptive-analysis-of-confidence-and-training
#9
Dinah J Gould, Jane Chudleigh, Edward Purssell, Clare Hawker, Sarah Gaze, Deborah James, Mary Lynch, Nicola Pope, Nicholas Drey
BACKGROUND: In many countries, aseptic procedures are undertaken by nurses in the general ward setting, but variation in practice has been reported, and evidence indicates that the principles underpinning aseptic technique are not well understood. METHODS: A survey was conducted, employing a brief, purpose-designed, self-reported questionnaire. RESULTS: The response rate was 72%. Of those responding, 65% of nurses described aseptic technique in terms of the procedure used to undertake it, and 46% understood the principles of asepsis...
November 21, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/29156617/optimum-thermal-processing-for-extended-shelf-life-esl-milk
#10
REVIEW
Hilton Deeth
Extended shelf-life (ESL) or ultra-pasteurized milk is produced by thermal processing using conditions between those used for traditional high-temperature, short-time (HTST) pasteurization and those used for ultra-high-temperature (UHT) sterilization. It should have a refrigerated shelf-life of more than 30 days. To achieve this, the thermal processing has to be quite intense. The challenge is to produce a product that has high bacteriological quality and safety but also very good organoleptic characteristics...
November 20, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29142577/congenital-hypothyroidism-in-neonates-of-a-tertiary-care-hospital
#11
Adeel Ahmad, Anam Wasim, Shahida Hussain, Muhammad Saeed, Bilal Munir Ahmad, Khalil Ur Rehman
Objective: To determine neonatal congenital hypothyroidism among neonates born in a tertiary care hospital of Lahore Pakistan. Methods: This cross-sectional study was carried out at Pathology Department of Allama Iqbal Medical College, Lahore in collaboration with Pediatrics and Gynecology & Obstetrics Department, Jinnah Hospital, Lahore Pakistan. A total of 770 babies were included in this study, both male and female. About 2 ml venous blood samples were collected aseptically from the neonates in sterile clotted tube...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29130033/danger-of-frustrated-sensors-role-of-toll-like-receptors-and-nod-like-receptors-in-aseptic-and-septic-inflammations-around-total-hip-replacements
#12
Michiaki Takagi, Yuya Takakubo, Jukka Pajarinen, Yasushi Naganuma, Hiroharu Oki, Masahiro Maruyama, Stuart B Goodman
The innate immune sensors, Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs), can recognize not only exogenous pathogen-associated molecular patterns (PAMPs), but also endogenous molecules created upon tissue injury, sterile inflammation, and degeneration. Endogenous ligands are called damage-associated molecular patterns (DAMPs), and include endogenous molecules released from activated and necrotic cells as well as damaged extracellular matrix. TLRs and NLRs can interact with various ligands derived from PAMPs and DAMPs, leading to activation and/or modulation of intracellular signalling pathways...
July 2017: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29084027/antimicrobial-efficacy-of-materials-used-for-sealing-the-implant-abutment-screw-hole-an-in-vitro-evaluation
#13
Mohammed Alshehri, Hamad Albaqiah
AIM: The aim of this in vitro study was to assess the antimicrobial efficacy of materials (gutta percha [GP], polytetrafluoroethylene [PTFE] tape, and vinyl polysiloxane [VP]) used for sealing the implant abutment screw hole (IASH). METHODS: Sixty implants with a Morse cone connection were used. The implants were randomly divided into 3 groups (20 implants/group). In groups 1, 2, and 3, the IASH was sealed with GP, PTFE, and VP, respectively. Unstimulated whole saliva (UWS) was used as a contaminant medium for the incubation of the specimens...
December 2017: Implant Dentistry
https://www.readbyqxmd.com/read/29044722/alopecic-and-aseptic-nodule-of-the-scalp-in-a-girl
#14
Erika Rodríguez-Lobato, Daniel Morgado-Carrasco, Priscila Giavedoni, Juan Ferrando
Alopecic and aseptic nodule of the scalp is a rare entity characterized by the presence of nodules or cysts with sterile punctured material and negative cultures accompanied by nonscarring alopecia in the scalp of young men. We describe a case in which an 11-year-old girl presented with a nodular, fluctuant, round lesion on the vertex with localized alopecia. High-resolution ultrasound showed a hypoechoic lesion with increased flow on Doppler imaging and culture of the citrine-yellowish material obtained by puncture was negative...
October 16, 2017: Pediatric Dermatology
https://www.readbyqxmd.com/read/29038974/bioreactors-based-on-immobilized-fungi-bioremediation-under-non-sterile-conditions
#15
REVIEW
Kateřina Svobodová, Čeněk Novotný
White-rot fungi are renowned for their remarkable potential to degrade a wide range of organic pollutants. They are applicable in standard bioreactors offering both the use of the continuous mode of action and easy upscaling of the biodegradation process. The recent advance in this field consisted in the use of various fungi and different types of reactors in the treatment of real wastewaters. Most degradation studies involving white-rot fungi carried out so far used controlled, aseptic conditions. However, during bioremediation of real wastewaters, the degradation capacity of the fungi would be significantly affected by autochthonous microorganisms...
October 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28941559/infection-prevention-strategies-for-procedures-performed-outside-operating-rooms-a-conceptual-integrated-model
#16
Vincent Masse, Michael B Edmond, Daniel J Diekema
No comprehensive guidelines or classification exist for infection prevention strategies for medical procedures performed outside operating rooms. We reviewed the available literature and used our clinical experience to develop a progressive, 5-tiered classification of procedures, encompassing clean, aseptic, sterile-superficial, sterile-invasive, and surgical-like procedures to address this need. We provide a description of these categories, along with relevant examples. We fully acknowledge the limitations of our work, which is intended as a starting point for future efforts and not to be definitive...
September 20, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28911843/the-effect-of-a-cannula-milk-sampling-technique-on-the-microbiological-diagnosis-of-bovine-mastitis
#17
M Friman, H Hiitiö, M Niemi, J Holopainen, S Pyörälä, H Simojoki
Two methods of collecting milk samples from mastitic bovine mammary quarters were compared. Samples were taken in a consistent order in which standard aseptic technique sampling was done first, followed by insertion of a sterile cannula through the teat canal and collection of a second sample. Microbiological results of those two sampling techniques were compared. Milk samples were analysed using multiplex real-time polymerase chain reaction (PCR). The cannula technique produced a reduced number of microbial species or groups of species per sample compared with conventional sampling...
August 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28865636/clinical-issues-september-2017
#18
Amber Wood
Performing Staphylococcus aureus decolonization for urgent procedures Key words: decolonization, Staphylococcus aureus, MRSA, MSSA, mupirocin. Hand hygiene before donning gloves Key words: hand hygiene, glove, personal protective equipment. Hand hygiene before skin prep Key words: hand hygiene, skin prep, sterile task, aseptic task. Scrubbing by stroke versus timed method Key words: surgical hand rub, surgical scrub, stroke method, timed method.
September 2017: AORN Journal
https://www.readbyqxmd.com/read/28856108/high-frequency-regeneration-of-plants-from-cotyledon-and-hypocotyl-cultures-in-brassica-oleracea-cv-pride-of-india
#19
Geetika Gambhir, Pankaj Kumar, D K Srivastava
Morphogenic potential of cabbage cv. Pride of India, for multiple shoot induction was tested under in vitro conditions using cotyledon and hypocotyl explants. Aseptically grown seven to nine days old seedlings of cabbage were used as source of explants for reproducible plant regeneration studies. Forty different concentrations and combinations of TDZ (alone), TDZ with adenine, TDZ with NAA and TDZ with IAA were tried. Maximum shoot regeneration response from cotyledon explants (91.11%) and hypocotyl (94.40%) was obtained on MS medium containing 0...
September 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28855842/efficient-and-reproducible-in-vitro-regeneration-of-solanum-lycopersicum-and-assessment-genetic-uniformity-using-flow-cytometry-and-spar-methods
#20
Abdulrahman A Alatar, Mohammad Faisal, Eslam M Abdel-Salam, Tomas Canto, Quaiser Saquib, Saad B Javed, Mohamed A El-Sheikh, Abdulaziz A Al-Khedhairy
In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta box moistened with sterile half strength (½)Murashige and Skoog (MS) medium. Regeneration efficiency of cotyledonary leaf (CL) and cotyledonary node (CN) explants derived from 08 days old aseptic seedling were assessed on MS medium supplemented with different concentrations of auxins and cytokinin...
September 2017: Saudi Journal of Biological Sciences
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