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https://www.readbyqxmd.com/read/29142577/congenital-hypothyroidism-in-neonates-of-a-tertiary-care-hospital
#1
Adeel Ahmad, Anam Wasim, Shahida Hussain, Muhammad Saeed, Bilal Munir Ahmad, Khalil Ur Rehman
Objective: To determine neonatal congenital hypothyroidism among neonates born in a tertiary care hospital of Lahore Pakistan. Methods: This cross-sectional study was carried out at Pathology Department of Allama Iqbal Medical College, Lahore in collaboration with Pediatrics and Gynecology & Obstetrics Department, Jinnah Hospital, Lahore Pakistan. A total of 770 babies were included in this study, both male and female. About 2 ml venous blood samples were collected aseptically from the neonates in sterile clotted tube...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29130033/danger-of-frustrated-sensors-role-of-toll-like-receptors-and-nod-like-receptors-in-aseptic-and-septic-inflammations-around-total-hip-replacements
#2
Michiaki Takagi, Yuya Takakubo, Jukka Pajarinen, Yasushi Naganuma, Hiroharu Oki, Masahiro Maruyama, Stuart B Goodman
The innate immune sensors, Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs), can recognize not only exogenous pathogen-associated molecular patterns (PAMPs), but also endogenous molecules created upon tissue injury, sterile inflammation, and degeneration. Endogenous ligands are called damage-associated molecular patterns (DAMPs), and include endogenous molecules released from activated and necrotic cells as well as damaged extracellular matrix. TLRs and NLRs can interact with various ligands derived from PAMPs and DAMPs, leading to activation and/or modulation of intracellular signalling pathways...
July 2017: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29084027/antimicrobial-efficacy-of-materials-used-for-sealing-the-implant-abutment-screw-hole-an-in-vitro-evaluation
#3
Mohammed Alshehri, Hamad Albaqiah
AIM: The aim of this in vitro study was to assess the antimicrobial efficacy of materials (gutta percha [GP], polytetrafluoroethylene [PTFE] tape, and vinyl polysiloxane [VP]) used for sealing the implant abutment screw hole (IASH). METHODS: Sixty implants with a Morse cone connection were used. The implants were randomly divided into 3 groups (20 implants/group). In groups 1, 2, and 3, the IASH was sealed with GP, PTFE, and VP, respectively. Unstimulated whole saliva (UWS) was used as a contaminant medium for the incubation of the specimens...
October 27, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/29044722/alopecic-and-aseptic-nodule-of-the-scalp-in-a-girl
#4
Erika Rodríguez-Lobato, Daniel Morgado-Carrasco, Priscila Giavedoni, Juan Ferrando
Alopecic and aseptic nodule of the scalp is a rare entity characterized by the presence of nodules or cysts with sterile punctured material and negative cultures accompanied by nonscarring alopecia in the scalp of young men. We describe a case in which an 11-year-old girl presented with a nodular, fluctuant, round lesion on the vertex with localized alopecia. High-resolution ultrasound showed a hypoechoic lesion with increased flow on Doppler imaging and culture of the citrine-yellowish material obtained by puncture was negative...
October 16, 2017: Pediatric Dermatology
https://www.readbyqxmd.com/read/29038974/bioreactors-based-on-immobilized-fungi-bioremediation-under-non-sterile-conditions
#5
REVIEW
Kateřina Svobodová, Čeněk Novotný
White-rot fungi are renowned for their remarkable potential to degrade a wide range of organic pollutants. They are applicable in standard bioreactors offering both the use of the continuous mode of action and easy upscaling of the biodegradation process. The recent advance in this field consisted in the use of various fungi and different types of reactors in the treatment of real wastewaters. Most degradation studies involving white-rot fungi carried out so far used controlled, aseptic conditions. However, during bioremediation of real wastewaters, the degradation capacity of the fungi would be significantly affected by autochthonous microorganisms...
October 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28941559/infection-prevention-strategies-for-procedures-performed-outside-operating-rooms-a-conceptual-integrated-model
#6
Vincent Masse, Michael B Edmond, Daniel J Diekema
No comprehensive guidelines or classification exist for infection prevention strategies for medical procedures performed outside operating rooms. We reviewed the available literature and used our clinical experience to develop a progressive, 5-tiered classification of procedures, encompassing clean, aseptic, sterile-superficial, sterile-invasive, and surgical-like procedures to address this need. We provide a description of these categories, along with relevant examples. We fully acknowledge the limitations of our work, which is intended as a starting point for future efforts and not to be definitive...
September 20, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28911843/the-effect-of-a-cannula-milk-sampling-technique-on-the-microbiological-diagnosis-of-bovine-mastitis
#7
M Friman, H Hiitiö, M Niemi, J Holopainen, S Pyörälä, H Simojoki
Two methods of collecting milk samples from mastitic bovine mammary quarters were compared. Samples were taken in a consistent order in which standard aseptic technique sampling was done first, followed by insertion of a sterile cannula through the teat canal and collection of a second sample. Microbiological results of those two sampling techniques were compared. Milk samples were analysed using multiplex real-time polymerase chain reaction (PCR). The cannula technique produced a reduced number of microbial species or groups of species per sample compared with conventional sampling...
August 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28865636/clinical-issues-september-2017
#8
Amber Wood
Performing Staphylococcus aureus decolonization for urgent procedures Key words: decolonization, Staphylococcus aureus, MRSA, MSSA, mupirocin. Hand hygiene before donning gloves Key words: hand hygiene, glove, personal protective equipment. Hand hygiene before skin prep Key words: hand hygiene, skin prep, sterile task, aseptic task. Scrubbing by stroke versus timed method Key words: surgical hand rub, surgical scrub, stroke method, timed method.
September 2017: AORN Journal
https://www.readbyqxmd.com/read/28856108/high-frequency-regeneration-of-plants-from-cotyledon-and-hypocotyl-cultures-in-brassica-oleracea-cv-pride-of-india
#9
Geetika Gambhir, Pankaj Kumar, D K Srivastava
Morphogenic potential of cabbage cv. Pride of India, for multiple shoot induction was tested under in vitro conditions using cotyledon and hypocotyl explants. Aseptically grown seven to nine days old seedlings of cabbage were used as source of explants for reproducible plant regeneration studies. Forty different concentrations and combinations of TDZ (alone), TDZ with adenine, TDZ with NAA and TDZ with IAA were tried. Maximum shoot regeneration response from cotyledon explants (91.11%) and hypocotyl (94.40%) was obtained on MS medium containing 0...
September 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28855842/efficient-and-reproducible-in-vitro-regeneration-of-solanum-lycopersicum-and-assessment-genetic-uniformity-using-flow-cytometry-and-spar-methods
#10
Abdulrahman A Alatar, Mohammad Faisal, Eslam M Abdel-Salam, Tomas Canto, Quaiser Saquib, Saad B Javed, Mohamed A El-Sheikh, Abdulaziz A Al-Khedhairy
In the present study, we develop an efficient and reproducible in vitro regeneration system for two cultivars viz., Jamila and Tomaland of Solanum lycopersicum L., an economically important vegetable crop throughout the world. Sterilization of seeds with 2.5% (v/v) NaOCl was found to be most effective, about 97% of seeds germinated on cotton in magenta box moistened with sterile half strength (½)Murashige and Skoog (MS) medium. Regeneration efficiency of cotyledonary leaf (CL) and cotyledonary node (CN) explants derived from 08 days old aseptic seedling were assessed on MS medium supplemented with different concentrations of auxins and cytokinin...
September 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28799106/chemical-sterilization-of-allograft-dermal-tissues
#11
Abigail Phipps, Edward Vaynshteyn, John B Kowalski, Manh-Dan Ngo, Karen Merritt, Joel Osborne, Evangelia Chnari
Common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. One approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. This combination of processes is an accepted method for production of sterile healthcare products as described in ANSI/AAMI ST67:2011. This article describes the validation of the peracetic acid and ethanol-based (PAAE) chemical sterilization process for allograft dermal tissues at the Musculoskeletal Transplant Foundation (MTF, Edison, NJ)...
December 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28796279/withdrawn-intermittent-catheterisation-for-long-term-bladder-management
#12
REVIEW
Jacqui Prieto, Catherine L Murphy, Katherine N Moore, Mandy Fader
BACKGROUND: Intermittent catheterisation is a commonly recommended procedure for people with incomplete bladder emptying. There are now several designs of intermittent catheter (e.g. different lengths, 'ready to use' presentation) with different materials (e.g. PVC-free) and coatings (e.g. hydrophilic). The most frequent complication of intermittent catheterisation is urinary tract infection (UTI), but satisfaction, preference and ease of use are also important to users. It is unclear which catheter designs, techniques or strategies affect the incidence of UTI, which are preferable to users and which are most cost effective...
August 8, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28758156/varicella-zoster-virus-and-large-vessel-vasculitis-the-absence-of-an-association
#13
Gary W Procop, Charis Eng, Alison Clifford, Alexandra Villa-Forte, Leonard H Calabrese, Eric Roselli, Lars Svensson, Douglas Johnston, Gosta Pettersson, Edward Soltesz, Lisa Lystad, Julian D Perry, Alexander Blandford, Deborah A Wilson, Gary S Hoffman
OBJECTIVE: It is controversial whether microorganisms play a role in the pathogenesis of large and medium vessel vasculitides (eg, giant cell arteritis [GCA], Takayasu arteritis [TAK] and focal idiopathic aortitis [FIA]). Recent studies have reported the presence of Varicella Zoster Virus (VZV) within formalin-fixed, paraffin-embedded temporal arteries and aortas of about three-quarters or more of patients with these conditions, and in a minority of controls. In a prospective study, we sought to confirm these findings using DNA extracted from vessels that were harvested under surgically aseptic conditions and snap frozen...
2017: Pathogens & Immunity
https://www.readbyqxmd.com/read/28748050/assessment-of-facultative-anaerobes-from-the-root-canals-of-deciduous-molars-an-in-vivo-study
#14
Manisha Chandwani, Shweta Chandak
Background. The current research aimed to assess the prevalence of facultative anaerobes isolated from the root canals of deciduous molars. Methods. The present research enrolled 60 children in the 6‒9-year age group based on clinical and radiographic findings. Under aseptic conditions, access cavities were prepared followed by collection of samples from infected root canals with the help of sterile paper points. The samples thus obtained were subjected for microbial assay. Results. It was found that Enterococcus faecalis was isolated in 30% of cases, Escherichia coli in 28...
2017: Journal of Dental Research, Dental Clinics, Dental Prospects
https://www.readbyqxmd.com/read/28745283/fabrication-of-biopolymer-based-nanocomposite-wound-dressing-evaluation-of-wound-healing-properties-and-wound-microbial-load
#15
Ali Es-Haghi, Mohammad Mashreghi, Mahere Rezazade Bazaz, Masoud Homayouni-Tabrizi, Majid Darroudi
The aim of this study is to introduce natural-based polymers, chitosan and starch, to design a remedial nanocomposite, comprising of cerium oxide nanoparticles and silver nanoparticles, to investigate their effects in accelerating wound healing and in wound microbial load. Cerium oxide nanoparticles synthesized in starch solution added to the colloidal dispersion of synthesized silver nanoparticles in chitosan to make a three-component nanomaterial. Mice were anaesthetized and two parallel full-thickness round wounds were excised under aseptic conditions with the help of sterile dermal biopsy punch...
August 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28724496/effects-of-time-and-storage-conditions-on-the-chemical-and-microbiologic-stability-of-diluted-buprenorphine-for-injection
#16
Johnathan M DenHerder, Ralph L Reed, Jennifer L Sargent, Gerd Bobe, Jan F Stevens, Helen E Diggs
Buprenorphine is a partial μ-opioid agonist used for analgesia. Due to the small size of laboratory rodents, buprenorphine HCl is typically diluted 10- or 20-fold with a sterile diluent, such as saline, for accurate dosing. Protocols for preparing and storing diluted buprenorphine vary by institution, and little published information is available regarding stability and beyond-use dating of specific buprenorphine preparations. The purpose of this study was to determine the chemical and microbiologic stability of diluted buprenorphine stored for a maximum of 180 d...
July 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28717318/hemogram-responses-in-goats-toward-challenged-with-corynebacterium-pseudotuberculosis-and-its-immunogen-mycolic-acids
#17
Mohammed Naji Odhah, Faez Firdaus Jesse Abdullah, Abd Wahid Haron, Mohd Azmi Mohd Lila, Mohd Zamri-Saad, Zaid Khuder, Idris Umar Hambali, Muhammed Umar, Wessam Monther Saleh
AIM: This study was conducted to analyze the changes in blood profile of goats inoculated with Corynebacterium pseudotuberculosis and its immunogen mycolic acid (MA) extract. MATERIALS AND METHODS: A total of 12 clinically healthy crossbred Boer female goats were divided into three groups; A, B and C (4 goats each per group). Group A was inoculated with 2 ml sterile phosphate buffered saline via intradermal route as the negative control group whilst Group B was inoculated with 2 ml of MA extract (1 g/ml) intradermally and Group C was then inoculated with 2 ml (1×10(9)) colony forming unit of active C...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28714050/superiority-of-the-sonication-method-against-conventional-periprosthetic-tissue-cultures-for-diagnosis-of-prosthetic-joint-infections
#18
Sofia Tani, Panagiotis Lepetsos, Antonios Stylianakis, John Vlamis, Konstantinos Birbas, Ioannis Kaklamanos
INTRODUCTION: Diagnosis of periprosthetic infections is challenging. The aim of this study was to compare the diagnostic accuracy of conventional periprosthetic tissue culture and culture of sonication fluid of the explanted prostheses. METHODS: We prospectively enrolled 114 patients undergoing revision hip or knee arthroplasty because of loosening of the prostheses, at our institution, between July 2012 and July 2016. Patients' medical history and demographic characteristics were recorded...
July 17, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/28691448/evaluation-of-chemical-physical-properties-and-cytocompatibility-of-theracal-lc
#19
A Buonavoglia, D Lauritano, D Perrone, F Ardito, G Troiano, M Dioguardi, V Candotto, F J Silvestre, L Lo Muzio
TheraCal LC (TLC, Bisco Inc., Schaumburg, IL, USA) is a light-cured, resin-modified, calcium silicate-filled base/liner material designed for direct and indirect pulp-capping. In this study the result of the evaluation in vitro of the biocompatibility and chemical-physical properties of TLC are reported. TLC specimens were prepared under aseptic conditions in strict compliance with the manufacturer’s instructions and sterilized. Osteoblast-like cells (MG63) were used. For different time points, solubility, water uptake, alkalinizing activity and cytotoxicity were evaluated...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28665993/knowledge-attitudes-and-practice-on-the-prevention-of-central-line-associated-bloodstream-infections-among-nurses-in-oncological-care-a-cross-sectional-study-in-an-area-of-southern-italy
#20
Maria Rosaria Esposito, Assunta Guillari, Italo Francesco Angelillo
The objectives of the cross-sectional study were to delineate the knowledge, attitudes, and behavior among nurses regarding the prevention of central line-associated bloodstream infections (CLABSIs) and to identify their predisposing factors. A questionnaire was self-administered from September to November 2011 to nurses in oncology and outpatient chemotherapy units in 16 teaching and non-teaching public and private hospitals in the Campania region (Italy). The questionnaire gathered information on demographic and occupational characteristics; knowledge about evidence-based practices for the prevention of CLABSIs; attitudes towards guidelines, the risk of transmitting infections, and hand-washing when using central venous catheter (CVC); practices about catheter site care; and sources of information...
2017: PloS One
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