Dan Huang, Li Zhang, Ying Liu, Ji Wang, Jie Zhang, Katherine J Baines, Gang Liu, Alan Chen-Yu Hsu, Fang Wang, Zhihong Chen, Brian G Oliver, Min Xie, Ling Qin, Dan Liu, Huajing Wan, Fengming Luo, Weimin Li, Gang Wang, Peter G Gibson
BACKGROUND: Non-neuronal cholinergic system (NNCS) contributes to various inflammatory airway diseases. However, the role of NNCS in severe asthma (SA) remains largely unexplored. OBJECTIVE: To explore airway NNCS in SA. METHODS: In this prospective cohort study based on the Australasian Severe Asthma Network in a real-world setting, patients with SA (n=52) and non-SA (n=104) underwent clinical assessment and sputum induction. The mRNA levels of NNCS components and proinflammatory cytokines in sputum were detected using RT-qPCR, and the concentrations of acetylcholine (Ach)-related metabolites were examined using LC-MS/MS...
March 16, 2024: Annals of Allergy, Asthma & Immunology