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https://www.readbyqxmd.com/read/28327345/immunoassays-for-riboflavin-and-flavin-mononucleotide-using-antibodies-specific-to-d-ribitol-and-d-ribitol-5-phosphate
#1
G Ravi, Yeldur P Venkatesh
Riboflavin (vitamin B2), a water-soluble vitamin, plays a key role in maintaining human health. Though, numerous methods have been reported for the determination of total riboflavin (TRF) content in foods and biological samples, very few methods are reported for quantifying riboflavin and its coenzymes [flavin mononucleotide (FMN); flavin adenine dinucleotide (FAD)] individually. Recently, we have demonstrated that antibodies specific to d-ribitol and d-ribitol-5-phosphate also recognize riboflavin and FMN, respectively, and not vice-versa...
March 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28320616/high-content-screen-using-zebrafish-danio-rerio-embryos-identifies-a-novel-kinase-activator-and-inhibitor
#2
Werner J Geldenhuys, Sadie A Bergeron, Jackie E Mullins, Rowaa Aljammal, Briah L Gaasch, Wei-Chi Chen, June Yun, Lori A Hazlehurst
In this report we utilized zebrafish (Danio rerio) embryos in a phenotypical high-content screen (HCS) to identify novel leads in a cancer drug discovery program. We initially validated our HCS model using the flavin adenosine dinucleotide (FAD) containing endoplasmic reticulum (ER) enzyme, endoplasmic reticulum oxidoreductase (ERO1) inhibitor EN460. EN460 showed a dose response effect on the embryos with a dose of 10μM being significantly lethal during early embryonic development. The HCS campaign which employed a small library identified a promising lead compound, a naphthyl-benzoic acid derivative coined compound 1 which had significant dosage and temporally dependent effects on notochord and muscle development in zebrafish embryos...
February 28, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28318160/-phonological-characteristics-and-rehabilitation-training-of-abnormal-velar-in-children-with-functional-articulation-disorders
#3
Xu Lina, Li Feng, Zhang Yanyun, Gao Nan, Hu Mingfang
OBJECTIVE: To explore the phonological characteristics and rehabilitation training of abnormal velar in patients with functional articulation disorders (FAD). METHODS: Eighty-seven patients with FAD were observed of the phonological characteristics of velar. Seventy-two patients with abnormal velar accepted speech training. The correlation and simple linear regression analysis were carried out on abnormal velar articulation and age. RESULTS: The articulation disorder of /g/ mainly showed replacement by /d/, /b/ or omission...
December 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28316889/functional-and-health-promoting-inherent-attributes-of-enterococcus-hirae-f2-as-a-novel-probiotic-isolated-from-the-digestive-tract-of-the-freshwater-fish-catla-catla
#4
Mohd Adnan, Mitesh Patel, Sibte Hadi
BACKGROUND: Probiotic microorganisms are gaining global importance because of their use in the preparation of a nutraceutical or in the treatment of infections. As per the health industry demand, there is an urgent need for exploring new indigenous probiotic strains with its specific origin due to variation in gut microflora, different food habits and specific host-microbial interactions. The main objective of the present study was to isolate and identify a novel probiotic Enterococcus strain from the gut of Catla catla fish and evaluate its potentiality as a potent probiotic...
2017: PeerJ
https://www.readbyqxmd.com/read/28296892/blue-light-induced-accumulation-of-reactive-oxygen-species-is-a-consequence-of-the-drosophila-cryptochrome-photocycle
#5
Louis-David Arthaut, Nathalie Jourdan, Ali Mteyrek, Maria Procopio, Mohamed El-Esawi, Alain d'Harlingue, Pierre-Etienne Bouchet, Jacques Witczak, Thorsten Ritz, André Klarsfeld, Serge Birman, Robert J Usselman, Ute Hoecker, Carlos F Martino, Margaret Ahmad
Cryptochromes are evolutionarily conserved blue-light absorbing flavoproteins which participate in many important cellular processes including in entrainment of the circadian clock in plants, Drosophila and humans. Drosophila melanogaster cryptochrome (DmCry) absorbs light through a flavin (FAD) cofactor that undergoes photoreduction to the anionic radical (FAD•-) redox state both in vitro and in vivo. However, recent efforts to link this photoconversion to the initiation of a biological response have remained controversial...
2017: PloS One
https://www.readbyqxmd.com/read/28295778/the-ricaft-ymca-ylbf-yaat-complex-carries-two-4fe-4s-2-clusters-and-may-respond-to-redox-changes
#6
Andrew W Tanner, Valerie J Carabetta, Ryan J Martinie, Ameya A Mashruwala, Jeffrey M Boyd, Carsten Krebs, David Dubnau
During times of environmental insult, Bacillus subtilis undergoes developmental changes leading to biofilm formation, sporulation and competence. Each of these states is regulated in part by the phosphorylated form of the master response regulator Spo0A (Spo0A∼P). The phosphorylation state of Spo0A is controlled by a multi-component phosphorelay. RicA, RicF and RicT (previously YmcA, YlbF and YaaT) have been shown to be important regulatory proteins for multiple developmental fates. These proteins directly interact and form a stable complex, which has been proposed to accelerate the phosphorelay...
March 15, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28294374/theoretical-approaches-for-predicting-the-color-of-rigid-dyes-in-solution
#7
Stefania Di Tommaso, Diane Bousquet, Delphine Moulin, Frédéric Baltenneck, Priscilla Riva, Hervé David, Aziz Fadli, Jérôme Gomar, Ilaria Ciofini, Carlo Adamo
Aiming at developing an affordable and easily implementable computational protocol for routine prediction of spectral properties of rigid molecular dyes, density functional theory, and time-dependent density functional theory were used in conjunction with a vibronic coupling scheme for band shape estimate. To predict the perceived color of molecules in solution, a model has been setup linking the UV-vis spectra predicted at ab initio level to the L*a*b* colorimetric parameters. The results show that a mixed protocol, implying the use of a global hybrid functional for the prediction of adiabatic energy differences and a range separated hybrid for the prediction of potential energy curvature, allows perceived colors to be quantitatively predicted, as demonstrated by the comparison of L*a*b* colorimetric parameters obtained from computed and experimental spectra...
March 10, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28291519/structural-studies-of-neurospora-crassa-lpmo9d-and-redox-partner-cdhiia-using-neutron-crystallography-and-small-angle-scattering
#8
Annette M Bodenheimer, William B O'Dell, Christopher B Stanley, Flora Meilleur
Sensitivity to hydrogen/deuterium and lack of observable radiation damage makes cold neutrons an ideal probe the structural studies of proteins with highly photosensitive groups such as the copper center of lytic polysaccharide monooxygenases (LPMOs) and flavin adenine dinucleotide (FAD) and heme redox cofactors of cellobiose dehydrogenases (CDHs). Here, neutron crystallography and small-angle neutron scattering are used to investigate Neurospora crassa LPMO9D (NcLPMO9D) and CDHIIA (NcCDHIIA), respectively...
March 4, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28291250/site-to-site-interdomain-communication-may-mediate-different-loss-of-function-mechanisms-in-a-cancer-associated-nqo1-polymorphism
#9
Encarnación Medina-Carmona, Jose L Neira, Eduardo Salido, Julian E Fuchs, Rogelio Palomino-Morales, David J Timson, Angel L Pey
Disease associated genetic variations often cause intracellular enzyme inactivation, dysregulation and instability. However, allosteric communication of mutational effects to distant functional sites leading to loss-of-function remains poorly understood. We characterize here interdomain site-to-site communication by which a common cancer-associated single nucleotide polymorphism (c.C609T/p.P187S) reduces the activity and stability in vivo of NAD(P)H:quinone oxidoreductase 1 (NQO1). NQO1 is a FAD-dependent, two-domain multifunctional stress protein acting as a Phase II enzyme, activating cancer pro-drugs and stabilizing p53 and p73α oncosuppressors...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287419/bimolecular-rate-constants-for-fad-dependent-glucose-dehydrogenase-from-aspergillus-terreus-and-organic-electron-acceptors
#10
Nozomu Tsuruoka, Takuya Sadakane, Rika Hayashi, Seiya Tsujimura
The flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) from Aspergillus species require suitable redox mediators to transfer electrons from the enzyme to the electrode surface for the application of bioelectrical devices. Although several mediators for FAD-GDH are already in use, they are still far from optimum in view of potential, kinetics, sustainability, and cost-effectiveness. Herein, we investigated the efficiency of various phenothiazines and quinones in the electrochemical oxidation of FAD-GDH from Aspergillus terreus...
March 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28279976/regulation-of-hypoxia-responses-by-flavin-adenine-dinucleotide-dependent-modulation-of-hif-1%C3%AE-protein-stability
#11
Suk-Jin Yang, Young Soo Park, Jung Hee Cho, Byul Moon, Hyun-Jung Ahn, Ju Yeon Lee, Zhi Xie, Yuli Wang, David Pocalyko, Dong Chul Lee, Hyun Ahm Sohn, Minho Kang, Jin Young Kim, Eunhee Kim, Kyung Chan Park, Jung-Ae Kim, Young Il Yeom
Oxygen deprivation induces a range of cellular adaptive responses that enable to drive cancer progression. Here, we report that lysine-specific demethylase 1 (LSD1) upregulates hypoxia responses by demethylating RACK1 protein, a component of hypoxia-inducible factor (HIF) ubiquitination machinery, and consequently suppressing the oxygen-independent degradation of HIF-1α. This ability of LSD1 is attenuated during prolonged hypoxia, with a decrease in the cellular level of flavin adenine dinucleotide (FAD), a metabolic cofactor of LSD1, causing HIF-1α downregulation in later stages of hypoxia...
March 9, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28279074/oxidized-derivatives-of-linoleic-acid-in-pediatric-metabolic-syndrome-is-their-pathogenic-role-modulated-by-the-genetic-background-and-the-gut-microbiota
#12
Domenico Trico, Anna Di Sessa, Sonia Caprio, Naga Chalasani, Wanqing Liu, Tiebing Liang, Joerg Graf, Raimund Herzog, Casey D Johnson, Giuseoppina Rosaria Umano, Ariel Feldstein, Nicola Santoro
AIMS: We tested whether oxidized metabolites of linoleic acid (OXLAM) are associated with pediatric metabolic syndrome (MetS) and a pro-atherogenic lipoprotein profile in 122 obese adolescents. Furthermore, we examined whether genetic and metagenomic factors can modulate plasma OXLAM concentrations by genotyping the Fatty Acid Desaturase 1/2 (FADS) gene and by characterizing the gut microbiota. RESULTS: Subjects with MetS (n=50) showed higher concentrations of 9- and 13-oxooctadecadienoic acid (9- and 13-oxo-ODE) than subjects without MetS (n=72)...
March 9, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28277707/family-assessment-device-real-world-validity-in-urban-families-of-children-with-asthma
#13
Gillian G Leibach, Robin S Everhart
Ecological momentary assessment (EMA) was used to further validate the real-world predictability of the Family Assessment Device (FAD) among low income, racial and ethnic minority, urban families of children (7-12 years) with asthma. Caregivers completed self-report measures at baseline, as well as daily assessments of family functioning for 2 weeks through EMA delivered via smartphone. Concurrent validity was established with measures of caregiver perceived stress and positive and negative affect at baseline...
March 9, 2017: Journal of Family Psychology: JFP
https://www.readbyqxmd.com/read/28274101/enhanced-purification-of-recombinant-rat-nadph-p450-reductase-by-using-a-hexahistidine-tag
#14
Hyoung-Goo Park, Young-Ran Lim, Songhee Han, Dabin Jeong, Donghak Kim
NADPH-P450 reductase (NPR) transfers electrons from NADPH to cytochrome P450 and heme oxygenase enzymes to support their catalytic activities. This protein is localized within the endoplasmic reticulum (ER) membrane and utilizes FMN, FAD, and NADPH as cofactors. While NPR is essential toward enabling the biochemical and pharmacological analyses of P450 enzymes, its production as a recombinant purified protein requires a series of tedious efforts and a high cost due to the use of NADP⁺ in the affinity chromatography process...
March 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28273563/discovery-of-highly-selective-and-potent-monoamine-oxidase-b-inhibitors-contribution-of-additional-phenyl-rings-introduced-into%C3%A2-2-aryl-1-3-4-oxadiazin-5-6h-one
#15
Jungeun Lee, Yeongcheol Lee, So Jung Park, Joohee Lee, Yeong Shik Kim, Young-Ger Suh, Jeeyeon Lee
Monoamine oxidase B (MAO-B) is a flavin adenine dinucleotide (FAD)-containing enzyme that plays a major role in the oxidative deamination of biogenic amines and neurotransmitters. Inhibiting MAO-B activity is a promising approach in the treatment of neurological disorders. Here, we report a series of 2-aryl-1,3,4-oxadiazin-5(6H)-one derivatives as highly selective and potent MAO-B inhibitors. Analysis of the binding sites of hMAO-A and hMAO-B led to design of linear analogs of 2-aryl-1,3,4-oxadiazin-5(6H)-one with an additional phenyl ring...
April 21, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28270505/essential-role-of-the-flexible-linker-on-the-conformational-equilibrium-of-bacterial-peroxiredoxin-reductase-for-effective-regeneration-of-peroxiredoxin
#16
Neelagandan Kamariah, Birgit Eisenhaber, Frank Eisenhaber, Gerhard Gruber
Reactive oxygen species (ROS) can damage DNA, proteins, and lipids, so cells have antioxidant systems that regulate ROS. In many bacteria, a dedicated peroxiredoxin reductase, alkyl hydroperoxide reductase subunit F (AhpF), catalyzes the rapid reduction of the redox-active disulfide center of the antioxidant protein peroxiredoxin (AhpC) to detoxify ROS such as hydrogen peroxide, organic hydroperoxide, and peroxynitrite. AhpF is a flexible multi-domain protein that enables a series of electron transfers among the redox centers by accepting reducing equivalents from NADH...
March 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28264344/a-review-of-subtribe-cymindidina-laporte-1834-coleoptera-carabidae-lebiini-in-southwestern-saudi-arabia-with-descriptions-of-two-new-species
#17
Iftekhar Rasool, Ron F F L Felix, Mahmoud S Abdel-Dayem, Hathal M Aldhafer
The carabid subtribe Cymindidina Laporte, 1834 is reviewed for the southwestern Saudi Arabia. Five species belonging to two genera (Afrotarus Jeannel, 1949 and Cymindis Latreille, 1806) are studied, including two species described as new: A. fadli sp. n. from Baha Province and A. soudaensis sp. n. from Asir Province. A key to genera and species of Saudi Arabia is given.
February 21, 2017: Zootaxa
https://www.readbyqxmd.com/read/28256579/proline-dehydrogenase-from-thermus-thermophilus-does-not-discriminate-between-fad-and-fmn-as-cofactor
#18
Mieke M E Huijbers, Marta Martínez-Júlvez, Adrie H Westphal, Estela Delgado-Arciniega, Milagros Medina, Willem J H van Berkel
Flavoenzymes are versatile biocatalysts containing either FAD or FMN as cofactor. FAD often binds to a Rossmann fold, while FMN prefers a TIM-barrel or flavodoxin-like fold. Proline dehydrogenase is denoted as an exception: it possesses a TIM barrel-like fold while binding FAD. Using a riboflavin auxotrophic Escherichia coli strain and maltose-binding protein as solubility tag, we produced the apoprotein of Thermus thermophilus ProDH (MBP-TtProDH). Remarkably, reconstitution with FAD or FMN revealed that MBP-TtProDH has no preference for either of the two prosthetic groups...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28246174/stilbene-epoxidation-and-detoxification-in-a-photorhabdus-luminescens-nematode-symbiosis
#19
Hyun Bong Park, Parthasarathy Sampathkumar, Corey E Perez, Joon Ha Lee, Jeannie Tran, Jeffrey B Bonanno, Elissa A Hallem, Steven C Almo, Jason M Crawford
Members of the Gammaproteobacterial Photorhabdus genus share mutualistic relationships with Heterorhabditis nematodes, and the pairs infect a wide swath of insect larvae. Photorhabdus species produce a family of stilbenes, with two major components being 3,5-dihydroxy-4-isopropyl-trans-stilbene (1) and its stilbene epoxide (2). This family of molecules harbors antimicrobial and immunosuppressive activities, and its pathway is responsible for producing a nematode ″food signal″ involved in nematode development...
February 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28237083/association-of-the-fads-gene-cluster-with-coronary-artery-disease-and-plasma-lipid-concentrations-in-the-northern-chinese-han-population
#20
Yixia Wu, Lixin Zeng, Xueyan Chen, Yang Xu, Lin Ye, Ling Qin, Liping Chen, Lin Xie
We investigated the association of single nucleotide polymorphisms (SNPs) in the fatty acid desaturase (FADS) gene cluster with coronary artery disease (CAD) in a case-control study and evaluated the possible influence of genetic variation on total cholesterol (TC) and triglyceride concentrations in the controls. In total, 497 CAD patients and 495 unrelated controls were genotyped for eight SNPs in the FADS gene cluster, and the blood lipid levels of subjects were measured. Three genetic models, including codominant, dominant and recessive, were used to analyze the genotypic relationship with CAD and plasma lipid levels...
February 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
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