keyword
MENU ▼
Read by QxMD icon Read
search

fad

keyword
https://www.readbyqxmd.com/read/28531287/bacterial-rna-polymerase-caps-rna-with-various-cofactors-and-cell-wall-precursors
#1
Christina Julius, Yulia Yuzenkova
Bacterial RNA polymerase is able to initiate transcription with adenosine-containing cofactor NAD+, which was proposed to result in a portion of cellular RNAs being 'capped' at the 5΄ end with NAD+, reminiscent of eukaryotic cap. Here we show that, apart from NAD+, another adenosine-containing cofactor FAD and highly abundant uridine-containing cell wall precursors, UDP-Glucose and UDP-N-acetylglucosamine are efficiently used to initiate transcription in vitro. We show that the affinity to NAD+ and UDP-containing factors during initiation is much lower than their cellular concentrations, and that initiation with them stimulates promoter escape...
May 22, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28529702/assessing-the-species-composition-of-tropical-eels-anguillidae-in-aceh-waters-indonesia-with-dna-barcoding-gene-cox1
#2
Zainal A Muchlisin, Agung Setia Batubara, Nur Fadli, Abdullah A Muhammadar, Afrita Ida Utami, Nurul Farhana, Mohd Nor Siti-Azizah
The objective of the present study was to evaluate the species diversity of eels native to Aceh waters based on genetic data. Sampling was conducted in western coast waters of Aceh Province, Indonesia, from July to August 2016. Genomic DNA was extracted from the samples, a genomic region from the 5' region of the cox1 gene was amplified and sequenced, and this was then used to analyse genetic variation. The genetic sequences were blasted into the NCBI database. Based on this analysis there were three valid species of eels that occurred in Aceh waters, namely Anguilla marmorata, A...
2017: F1000Research
https://www.readbyqxmd.com/read/28527736/the-effects-of-non-invasive-radiofrequency-electric-field-hyperthermia-on-biotransport-and-biodistribution-of-fluorescent-60-fullerene-derivative-in-a-murine-orthotopic-model-of-breast-adenocarcinoma
#3
Norman A Lapin, Martyna Krzykawska-Serda, Sean Dilliard, Yuri Mackeyev, Maciej Serda, Lon J Wilson, Steven A Curley, Stuart J Corr
The aim of this study is to understand the combined and differential biokinetic effects of radiofrequency (RF) electric-field hyperthermia as an adjunctive therapy to [60]fullerene nanoparticle-based drug delivery systems in targeting the micro-vasculature and micro-environments of breast cancer tumors. Intravital microscopy (IVM) is an ideal tool to provide the spatial and temporal resolution needed for quantification in this investigation. The water-soluble and fluorescent [60]fullerene derivative (C60-serPF) was designed to be an amphiphilic nanostructure, which is able to cross several biological membranes and accumulate in tumor tissues by passing through abnormally leaky tumor blood vessels...
May 17, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28526793/genomic-analysis-of-calderihabitans-maritimus-kkc1-a-thermophilic-hydrogenogenic-carboxydotrophic-bacterium-isolated-from-marine-sediment
#4
Kimiho Omae, Yasuko Yoneda, Yuto Fukuyama, Takashi Yoshida, Yoshihiko Sako
Calderihabitans maritimus KKC1 is a thermophilic hydrogenogenic carboxydotroph isolated from a submerged marine caldera. Here, we describe the de novo sequencing and feature analysis of the C. maritimus KKC1 genome. Genome-based phylogenetic analysis confirmed that C. maritimus KKC1 was most closely related to the genus Moorella, which includes well-studied acetogenic members. Comparative genomic analysis revealed that, like Moorella, C. maritimus KKC1 retained both the CO2-reducing Wood-Ljungdahl pathway and energy-converting hydrogenase-based module activated by reduced ferredoxin, but lacked the HydABC and NfnAB electron bifurcating enzymes and pyruvate:ferredoxin oxidoreductase required for ferredoxin reduction during acetogenic growth...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28525248/-magnetic-fields-and-health-from-epidemiology-to-cryptochrome-chemistry
#5
REVIEW
J Vanderstraeten
Biological effects of static magnetic fields (MF) and time-varying MF of electricity (50/60 Hz) appear possible from intensities in the low illitesla range. However, prolonged exposure to 50/60 Hz MF is associated with an increased risk of childhood leukemia at less than one microtesla of time-averaged intensity. And such kind of association is suggested in adults for some blood cancers and senile dementia. The cryptochrome hypothesis has been proposed to explain the association established with childhood leukemia...
2017: Revue Médicale de Bruxelles
https://www.readbyqxmd.com/read/28521794/in-vitro-synthesis-of-9-10-dihydroxyhexadecanoic-acid-using-recombinant-escherichia-coli
#6
Anees Kaprakkaden, Preeti Srivastava, Virendra Swarup Bisaria
BACKGROUND: Hydroxy fatty acids are widely used in food, chemical and cosmetic industries. A variety of dihydroxy fatty acids have been synthesized so far; however, no studies have been done on the synthesis of 9,10-dihydroxyhexadecanoic acid. In the present study recombinant E. coli has been used for the heterologous expression of fatty acid hydroxylating enzymes and the whole cell lysate of the induced culture was used for in vitro production of 9,10-dihydroxyhexadecanoic acid. RESULTS: A first of its kind proof of principle has been successfully demonstrated for the production of 9,10-dihydroxyhexadecanoic acid using three different enzymes viz...
May 18, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28515069/mendelian-randomization-shows-sex-specific-associations-between-long-chain-pufa-related-genotypes-and-cognitive-performance-in-danish-schoolchildren
#7
Lotte Lauritzen, Louise B Sørensen, Laurine B Harsløf, Christian Ritz, Ken D Stark, Arne Astrup, Camilla B Dyssegaard, Niels Egelund, Kim F Michaelsen, Camilla T Damsgaard
Background: Dietary and endogenously formed long-chain polyunsaturated fatty acids (LCPUFAs) are hypothesized to improve cognitive development, but results are inconclusive, with suggestions of sex specificity. One study suggested that single-nucleotide polymorphisms (SNPs) rs1535 and rs174448 in the fatty acid desaturase (FADS) gene cluster have opposite effects on erythrocyte LCPUFAs at 9 mo.Objective: To explore whether SNPs in FADS and elongase (ELOVL) genes were associated with school performance in a sex-specific manner, we performed a Mendelian randomization study using data from the Optimal well-being, development and health for Danish children through a healthy New Nordic Diet (OPUS) School Meal Study with 765 Danish schoolchildren 8-11 y old...
May 17, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28509930/elucidation-of-inhibitor-binding-pockets-of-d-amino-acid-oxidase-using-docking-simulation-and-n-sulfanylethylanilide-based-labeling-technology
#8
Taiki Kohiki, Yusuke Kato, Yusuke Nishikawa, Kazuko Yorita, Ikuko Sagawa, Masaya Denda, Tsubasa Inokuma, Akira Shigenaga, Kiyoshi Fukui, Akira Otaka
Because of the relevance of d-serine (d-Ser) to schizophrenia, inhibitors of d-amino acid oxidase (DAO), which catalyzes degradation of d-Ser in the presence of flavin adenine dinucleotide (FAD), are expected to be anti-schizophrenia therapeutics. In this study, binding pockets of DAO to its inhibitor 4-bromo-3-nitrobenzoic acid were searched by combining in silico docking simulation and labeling experiments employing an N-sulfanylethylanilide-based labeling technology that we have developed. The results clearly demonstrated that there are two binding pockets: one is shared with d-Ser and FAD, and the other is an unexpected cleft between the subunits of a DAO dimer...
May 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28508424/computational-site-directed-mutagenesis-studies-of-the-role-of-the-hydrophobic-triad-on-substrate-binding-in-cholesterol-oxidase
#9
Laith Hisham Harb, Mahreen Arooj, Alice Vrielink, Ricardo L Mancera
Cholesterol oxidase (ChOx) is a flavoenzyme that oxidises and isomerises cholesterol (CHL) to form cholest-4-en-3-one. Molecular docking and molecular dynamics simulations were conducted to predict the binding interactions of CHL in the active site. Several key interactions (E361-CHL, N485-FAD and H447-CHL) were identified and which are likely to determine the correct positioning of CHL relative to flavin-adenine dinucleotide (FAD). Binding of CHL also induced changes in key residues of the active site leading to the closure of the oxygen channel...
May 15, 2017: Proteins
https://www.readbyqxmd.com/read/28500705/integration-of-temperature-and-blue-light-sensing-in-acinetobacter-baumannii-through-the-blsa-sensor
#10
Inés Abatedaga, Lorena Valle, Adrián E Golic, Gabriela L Müller, Matías Cabruja, Faustino E Morán Vieyra, Paula C Jaime, María Alejandra Mussi, Claudio D Borsarelli
BlsA is a BLUF photoreceptor present in Acinetobacter baumannii, responsible for modulation of motility, biofilm formation and virulence by light. In this work, we have combined physiological and biophysical evidences to begin to understand the basis of the differential photoregulation observed as a function of temperature. Indeed, we show that blsA expression is reduced at 37°C, which correlates with negligible photoreceptor levels in the cells, likely accounting for absence of photoregulation at this temperature...
May 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28500402/identification-and-characterization-of-a-grain-micronutrient-related-osfro2-rice-gene-ortholog-from-micronutrient-rich-little-millet-panicum-sumatrense
#11
Girish Chandel, Mahima Dubey, Saurabh Gupta, Arun H Patil, A R Rao
Minor millets are considered as nutrient-rich cereals having significant effect in improving human health. In this study, a rice ortholog of Ferric Chelate Reductase (FRO2) gene involved in plant metal uptake has been identified in iron-rich Little millet (LM) using PCR and next generation sequencing-based strategy. FRO2 gene-specific primers designed from rice genome amplified 2.7 Kb fragment in LM genotype RLM-37. Computational genomics analyses of the sequenced amplicon showed high level sequence similarity with rice OsFRO2 gene...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28500115/erroneously-elevated-glucose-values-due-to-maltose-interference-in-mutant-glucose-dehydrogenase-pyrroloquinolinequinone-mutant-gdh-pqq-based-glucometer
#12
Partha Pratim Chakraborty, Shinjan Patra, Rana Bhattacharjee, Subhankar Chowdhury
Currently available glucose test strip enzymes include glucose oxidase (GOD) and glucose dehydrogenase (GDH). In GDH-based glucometers, glucose oxidation can be catalysed by different cofactors: nicotinamide adenine dinucleotide (GDH-NAD), flavin adenine dinucleotide (GDH-FAD), pyrroloquinolinequinone (GDH-PQQ) and mutant GDH-PQQ. GOD-based and GDH-NAD-based glucometers are substrate-specific and do not react with sugars other than glucose. GDH-FAD reacts with xylose only in addition to glucose. GDH-PQQ is not glucose-specific; in addition to glucose, it reacts with different other sugars and produces falsely high values of capillary glucose in the presence of such substances...
May 12, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28498544/metabolic-engineering-of-cofactor-flavin-adenine-dinucleotide-fad-synthesis-and-regeneration-in-escherichia-coli-for-production-of-%C3%AE-keto-acids
#13
Ying Hou, Gazi Sakir Hossain, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
Cofactor flavin adenine dinucleotide (FAD) plays a vital role in many FAD-dependent enzymatic reactions; therefore, how to efficiently accelerate FAD synthesis and regeneration is an important topic in biocatalysis and metabolic engineering. In this study, a system involving the synthesis pathway and regeneration of FAD was engineered in Escherichia coli to improve α-keto acid production-from the corresponding L-amino acids-catalyzed by FAD-dependent L-amino acid deaminase (L-AAD). First, key genes, ribH, ribC, and ribF, were overexpressed and fine-tuned for FAD synthesis...
May 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28495245/massive-parallel-sequencing-identifies-rapsn-and-pdha1-mutations-causing-fetal-akinesia-deformation-sequence
#14
Lore Winters, Evelien Van Hoof, Luc De Catte, Kris Van Den Bogaert, Thomy de Ravel, Liesbeth De Waele, Anniek Corveleyn, Jeroen Breckpot
INTRODUCTION: Fetal akinesia deformation sequence (FADS) or arthrogryposis multiplex congenita (AMC) is characterized by clinical ambiguity and genetic heterogeneity, hampering genetic diagnosis via traditional sequencing methods. Next generation sequencing (NGS) of all known disease-causing genes offers an elegant solution to identify the genetic etiology of AMC/FADS in a diagnostic setting. METHODS: An in-house developed disease-associated gene panel was conducted in two unrelated fetuses with FADS...
April 26, 2017: European Journal of Paediatric Neurology: EJPN
https://www.readbyqxmd.com/read/28485124/autofluorescence-imaging-identifies-tumor-cell-cycle-status-on-a-single-cell-level
#15
Tiffany M Heaster, Alex J Walsh, Yue Zhao, Scott W Hiebert, Melissa C Skala
The goal of this study is to validate fluorescence intensity and lifetime imaging of metabolic co-enzymes NAD(P)H and FAD (optical metabolic imaging, or OMI) as a method to quantify cell-cycle status of tumor cells. Heterogeneity in tumor cell-cycle status (e. g. proliferation, quiescence, apoptosis) increases drug resistance and tumor recurrence. Cell-cycle status is closely linked to cellular metabolism. Thus, this study applies cell-level metabolic imaging to distinguish proliferating, quiescent, and apoptotic populations...
May 9, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28479412/perceived-family-functioning-predicts-baseline-psychosocial-characteristics-in-u-s-participants-of-a-family-focused-grief-therapy-trial
#16
Tammy A Schuler, Talia I Zaider, Yuelin Li, Melissa Masterson, Glynnis A McDonnell, Shira Hichenberg, Rebecca Loeb, David W Kissane
CONTEXT AND OBJECTIVES: Screening and baseline data on 170 American families (620 individuals), selected by screening from a palliative care population for inclusion in a RCT of Family Focused Grief Therapy (FFGT), were examined to determine whether family dysfunction conferred higher levels of psychosocial morbidity. We hypothesized that greater family dysfunction would indeed be associated with poorer psychosocial outcomes among palliative care patients and their family members. METHODS: Screened families were classified according to their functioning on the Family Relationships Index (FRI) and consented families completed baseline assessments...
May 4, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28475111/riboflavin-responsive-mitochondrial-dysfunction-in-neurodegenerative-diseases
#17
REVIEW
Tamilarasan Udhayabanu, Andreea Manole, Mohan Rajeshwari, Perumal Varalakshmi, Henry Houlden, Balasubramaniem Ashokkumar
Mitochondria are the repository for various metabolites involved in diverse energy-generating processes, like the TCA cycle, oxidative phosphorylation, and metabolism of amino acids, fatty acids, and nucleotides, which rely significantly on flavoenzymes, such as oxidases, reductases, and dehydrogenases. Flavoenzymes are functionally dependent on biologically active flavin adenine dinucleotide (FAD) or flavin mononucleotide (FMN), which are derived from the dietary component riboflavin, a water soluble vitamin...
May 5, 2017: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/28464457/alzheimer-mouse-brain-tissue-measured-by-time-resolved-fluorescence-spectroscopy-using-single-and-multi-photon-excitation-of-label-free-native-molecules
#18
Bidyut Das, Lingyan Shi, Yury Budansky, Adrian Rodriguez-Contreras, Robert Alfano
Time resolved spectroscopic measurements with single-photon and multi-photon excitation of native molecules were performed ex vivo on brain tissues from an Alzheimer's disease (AD) and a wild type (WT) mouse model using a streak camera. The fluorescence decay times of native NADH and FAD show a longer relaxation time in AD than in WT tissue, suggesting less non-radiative processes in AD. The longer emission time of AD may be attributed to the coupling of the key native building block molecules to the amyloid-tau and/or to the caging of the native fluorophores by the deposition of amyloid-beta or tau plaques and neurofibrillary tangles that affect the local non-radiative interactions...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28464439/label-free-imaging-of-healthy-and-infarcted-fetal-sheep-hearts-by-two-photon-microscopy
#19
Alexandra Sorvina, Christie A Bader, Mitchell C Lock, Douglas A Brooks, Janna L Morrison, Sally E Plush
Coronary heart disease is one of the largest causes of death worldwide, making this a significant health care issue. A critical problem for the adult human heart is that it does not undergo effective repair in response to damage, leaving patients with a poor prognosis. Unlike the adult, fetal hearts have the ability to repair after myocardial damage. Using two-photon microscopy, we have visualised the morphological and metabolic changes following myocardial infarction in sheep fetuses, to characterise response to cardiac injury in a mammalian model...
May 2, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28463500/iruo-uses-an-fad-semiquinone-intermediate-for-iron-siderophore-reduction
#20
Marek J Kobylarz, Graham A Heieis, Slade A Loutet, Michael E P Murphy
Many pathogenic bacteria including Staphylococcus aureus use iron-chelating siderophores to acquire iron. IruO, an FAD-containing NADPH-dependent reductase from S. aureus, functions as a reductase for IsdG and IsdI, two paralogous heme degrading enzymes. Also, the gene encoding for IruO was shown to be required for growth of S. aureus on hydroxamate siderophores as a sole iron source. Here, we show that IruO binds the hydroxamate-type siderophores desferrioxamine B and ferrichrome A with low micromolar affinity and in the presence of NADPH, Fe(II) was released...
May 2, 2017: ACS Chemical Biology
keyword
keyword
82073
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"