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https://www.readbyqxmd.com/read/29043303/protein-dynamics-promote-hydride-tunnelling-in-substrate-oxidation-by-aryl-alcohol-oxidase
#1
Juan Carro, Marta Martínez-Júlvez, Milagros Medina, Angel T Martínez, Patricia Ferreira
The temperature dependence of hydride transfer from the substrate to the N5 of the FAD cofactor during the reductive half-reaction of Pleurotus eryngii aryl-alcohol oxidase (AAO) is assessed here. Kinetic isotope effects on both the pre-steady state reduction of the enzyme and its steady-state kinetics, with differently deuterated substrates, suggest an environmentally-coupled quantum-mechanical tunnelling process. Moreover, those kinetic data, along with the crystallographic structure of the enzyme in complex with a substrate analogue, indicate that AAO shows a pre-organized active site that would only require the approaching of the hydride donor and acceptor for the tunnelled transfer to take place...
October 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29040199/in-vitro-effect-of-lysozyme-on-albumin-deposition-to-hydrogel-contact-lens-materials
#2
Negar Babaei Omali, Lakshman N Subbaraman, Miriam Heynen, Zohra Fadli, Chantal Coles-Brennan, Lyndon W Jones
SIGNIFICANCE: Albumin deposition on contact lenses could be detrimental to contact lens (CL) wear because this may increase the risk of bacterial binding and reduce comfort. Lysozyme deposition on selected lens materials would reduce albumin deposition on lenses. PURPOSE: This study aims to determine if lysozyme deposition on CLs could act as a barrier against subsequent albumin adsorption, using an in vitro model. METHODS: Six hydrogel CL materials (etafilcon A, polymacon, nelfilcon A, omafilcon A, ocufilcon B, and nesofilcon A) were evaluated...
October 16, 2017: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/29032079/omega-6-fatty-acid-biomarkers-and-incident-type-2-diabetes-pooled-analysis-of-individual-level-data-for-39%C3%A2-740-adults-from-20-prospective-cohort-studies
#3
Jason H Y Wu, Matti Marklund, Fumiaki Imamura, Nathan Tintle, Andres V Ardisson Korat, Janette de Goede, Xia Zhou, Wei-Sin Yang, Marcia C de Oliveira Otto, Janine Kröger, Waqas Qureshi, Jyrki K Virtanen, Julie K Bassett, Alexis C Frazier-Wood, Maria Lankinen, Rachel A Murphy, Kalina Rajaobelina, Liana C Del Gobbo, Nita G Forouhi, Robert Luben, Kay-Tee Khaw, Nick Wareham, Anya Kalsbeek, Jenna Veenstra, Juhua Luo, Frank B Hu, Hung-Ju Lin, David S Siscovick, Heiner Boeing, Tzu-An Chen, Brian Steffen, Lyn M Steffen, Allison Hodge, Gudny Eriksdottir, Albert V Smith, Vilmunder Gudnason, Tamara B Harris, Ingeborg A Brouwer, Claudine Berr, Catherine Helmer, Cecilia Samieri, Markku Laakso, Michael Y Tsai, Graham G Giles, Tarja Nurmi, Lynne Wagenknecht, Matthias B Schulze, Rozenn N Lemaitre, Kuo-Liong Chien, Sabita S Soedamah-Muthu, Johanna M Geleijnse, Qi Sun, William S Harris, Lars Lind, Johan Ärnlöv, Ulf Riserus, Renata Micha, Dariush Mozaffarian
BACKGROUND: The metabolic effects of omega-6 polyunsaturated fatty acids (PUFAs) remain contentious, and little evidence is available regarding their potential role in primary prevention of type 2 diabetes. We aimed to assess the associations of linoleic acid and arachidonic acid biomarkers with incident type 2 diabetes. METHODS: We did a pooled analysis of new, harmonised, individual-level analyses for the biomarkers linoleic acid and its metabolite arachidonic acid and incident type 2 diabetes...
October 11, 2017: Lancet Diabetes & Endocrinology
https://www.readbyqxmd.com/read/29028002/cyanophage-encoded-lipid-desaturases-oceanic-distribution-diversity-and-function
#4
Sheila Roitman, Ellen Hornung, José Flores-Uribe, Itai Sharon, Ivo Feussner, Oded Béjà
Cyanobacteria are among the most abundant photosynthetic organisms in the oceans; viruses infecting cyanobacteria (cyanophages) can alter cyanobacterial populations, and therefore affect the local food web and global biochemical cycles. These phages carry auxiliary metabolic genes (AMGs), which rewire various metabolic pathways in the infected host cell, resulting in increased phage fitness. Coping with stress resulting from photodamage appears to be a central necessity of cyanophages, yet the overall mechanism is poorly understood...
October 13, 2017: ISME Journal
https://www.readbyqxmd.com/read/29024819/a-modular-metabolic-engineering-approach-for-the-production-of-1-2-propanediol-from-glycerol-by-saccharomyces-cerevisiae
#5
Zia-Ul Islam, Mathias Klein, Maximilian R Aßkamp, Anders S R Ødum, Elke Nevoigt
Compared to sugars, a major advantage of using glycerol as a feedstock for industrial bioprocesses is the fact that this molecule is more reduced than sugars. A compound whose biotechnological production might greatly profit from the substrate's higher reducing power is 1,2-propanediol (1,2-PDO). Here we present a novel metabolic engineering approach to produce 1,2-PDO from glycerol in S. cerevisiae. Apart from implementing the heterologous methylglyoxal (MG) pathway for 1,2-PDO formation from dihydroxyacetone phosphate (DHAP) and expressing a heterologous glycerol facilitator, the employed genetic modifications included the replacement of the native FAD-dependent glycerol catabolic pathway by the `DHA pathway´ for delivery of cytosolic NADH and the reduction of triosephosphate isomerase (TPI) activity for increased precursor (DHAP) supply...
October 9, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/29022241/a-panel-of-protein-candidates-for-comprehensive-study-of-caprine-arthritis-encephalitis-cae-infection
#6
Rosivaldo Quirino Bezerra Júnior, Ângela Maria Xavier Eloy, João Ricardo Furtado, Raymundo Rizaldo Pinheiro, Alice Andrioli, Frederico Bruno Moreno, Marina Duarte Pinto Lobo, Ana Cristina O Monteiro-Moreira, Renato de Azevedo Moreira, Tatiana Maria Farias Pinto, Maria Fátima da Silva Teixeira
The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR...
October 12, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28994401/crystal-structure-of-type-ii-nadh-quinone-oxidoreductase-from-caldalkalibacillus-thermarum-with-an-improved-resolution-of-2-15%C3%A2-%C3%A3
#7
Yoshio Nakatani, Wanting Jiao, David Aragão, Yosuke Shimaki, Jessica Petri, Emily J Parker, Gregory M Cook
Type II NADH:quinone oxidoreductase (NDH-2) is a respiratory enzyme found in the electron-transport chain of many species, with the exception of mammals. It is a 40-70 kDa single-subunit monotopic membrane protein that catalyses the oxidation of NADH and the reduction of quinone molecules via the cofactor FAD. NDH-2 is a promising new target for drug development given its essential role in many bacterial species and intracellular parasites. Only two bacterial NDH-2 structures have been reported and these structures are at moderate resolution (2...
October 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28987505/another-reason-to-avoid-the-gluten-free-fad
#8
EDITORIAL
Nihal K Patel, Brian E Lacy
No abstract text is available yet for this article.
October 5, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28985219/the-ins-and-outs-of-vanillyl-alcohol-oxidase-identification-of-ligand-migration-paths
#9
Gudrun Gygli, Maria Fátima Lucas, Victor Guallar, Willem J H van Berkel
Vanillyl alcohol oxidase (VAO) is a homo-octameric flavoenzyme belonging to the VAO/PCMH family. Each VAO subunit consists of two domains, the FAD-binding and the cap domain. VAO catalyses, among other reactions, the two-step conversion of p-creosol (2-methoxy-4-methylphenol) to vanillin (4-hydroxy-3-methoxybenzaldehyde). To elucidate how different ligands enter and exit the secluded active site, Monte Carlo based simulations have been performed. One entry/exit path via the subunit interface and two additional exit paths have been identified for phenolic ligands, all leading to the si side of FAD...
October 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28984838/application-of-negative-curvature-hollow-core-fiber-in-an-optical-fiber-sensor-setup-for-multiphoton-spectroscopy
#10
Maciej Andrzej Popenda, Hanna Izabela Stawska, Leszek Mateusz Mazur, Konrad Jakubowski, Alexey Kosolapov, Anton Kolyadin, Elżbieta Bereś-Pawlik
In this paper, an application of negative curvature hollow core fiber (NCHCF) in an all-fiber, multiphoton fluorescence sensor setup is presented. The dispersion parameter (D) of this fiber does not exceed the value of 5 ps/nm × km across the optical spectrum of (680-750) nm, making it well suited for the purpose of multiphoton excitation of biological fluorophores. Employing 1.5 m of this fiber in a simple, all-fiber sensor setup allows us to perform multiphoton experiments without any dispersion compensation methods...
October 6, 2017: Sensors
https://www.readbyqxmd.com/read/28974024/characterization-of-the-ornithine-hydroxylation-step-in-albachelin-biosynthesis
#11
Kendra Bufkin, Pablo Sobrado
N-Hydroxylating monooxygenases (NMOs) are involved in siderophore biosynthesis. Siderophores are high affinity iron chelators composed of catechol and hydroxamate functional groups that are synthesized and secreted by microorganisms and plants. Recently, a new siderophore named albachelin was isolated from a culture of Amycolatopsis alba growing under iron-limiting conditions. This work focuses on the expression, purification, and characterization of the NMO, abachelin monooxygenase (AMO) from A. alba. This enzyme was purified and characterized in its holo (FAD-bound) and apo (FAD-free) forms...
October 1, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28968240/families-affected-by-huntington-s-disease-report-difficulties-in-communication-emotional-involvement-and-problem-solving
#12
Celine M H Jona, Izelle Labuschagne, Emily-Clare Mercieca, Fiona Fisher, Cathy Gluyas, Julie C Stout, Sophie C Andrews
BACKGROUND: Family functioning in Huntington's disease (HD) is known from previous studies to be adversely affected. However, which aspects of family functioning are disrupted is unknown, limiting the empirical basis around which to create supportive interventions. OBJECTIVE: The aim of the current study was to assess family functioning in HD families. METHODS: We assessed family functioning in 61 participants (38 HD gene-expanded participants and 23 family members) using the McMaster Family Assessment Device (FAD; Epstein, Baldwin and Bishop, 1983), which provides scores for seven domains of functioning: Problem Solving; Communication; Affective Involvement; Affective Responsiveness; Behavior Control; Roles; and General Family Functioning...
2017: Journal of Huntington's Disease
https://www.readbyqxmd.com/read/28967629/mutations-in-aifm1-cause-an-x-linked-childhood-cerebellar-ataxia-partially-responsive-to-riboflavin
#13
G Heimer, E Eyal, X Zhu, E K Ruzzo, D Marek-Yagel, Doron Sagiv, Y Anikster, H Reznik-Wolf, E Pras, D Oz Levi, D Lancet, B Ben-Zeev, A Nissenkorn
BACKGROUND: AIFM1 encodes a mitochondrial flavoprotein with a dual role (NADH oxidoreductase and regulator of apoptosis), which uses riboflavin as a cofactor. Mutations in the X-linked AIFM1 were reported in relation to two main phenotypes: a severe infantile mitochondrial encephalomyopathy and an early-onset axonal sensorimotor neuropathy with hearing loss. In this paper we report two unrelated males harboring AIFM1 mutations (one of which is novel) who display distinct phenotypes including progressive ataxia which partially improved with riboflavin treatment...
September 15, 2017: European Journal of Paediatric Neurology: EJPN
https://www.readbyqxmd.com/read/28966143/functional-efficiency-of-mera-protein-among-diverse-mercury-resistant-bacteria-for-efficient-use-in-bioremediation-of-inorganic-mercury
#14
Hirak R Dash, Mousumi Sahu, Bibekanand Mallick, Surajit Das
MerA protein of mer operon in mercury resistant bacteria influences transformation of Hg(2+) to Hg(0). Both in-silico and in-vivo studies have been carried out and MerA sequences, conserved motifs for mercury binding and NADPH (GCVPSK and LSCCA) varied widely in both Gram-positive and Gram-negative bacteria. As MerA-NADPH-FAD complex plays an important role in mercury volatilization, molecular interaction studies between MerA, NADPH, FAD and Hg(2+) was carried out to study the efficiency of transformation of Hg(2+) to Hg(0) in mercury resistant bacteria...
September 28, 2017: Biochimie
https://www.readbyqxmd.com/read/28959184/mitophagy-failure-in-fibroblasts-and-ipsc-derived-neurons-of-alzheimer-s-disease-associated-presenilin-1-mutation
#15
Patricia Martín-Maestro, Ricardo Gargini, Andrew A Sproul, Esther García, Luis C Antón, Scott Noggle, Ottavio Arancio, Jesús Avila, Vega García-Escudero
Familial Alzheimer's disease (FAD) is clearly related with the accumulation of amyloid-beta (Aβ) and its deleterious effect on mitochondrial function is well established. Anomalies in autophagy have also been described in these patients. In the present work, functional analyses have been performed to study mitochondrial recycling process in patient-derived fibroblasts and neurons from induced pluripotent stem cells harboring the presenilin 1 mutation A246E. Mitophagy impairment was observed due to a diminished autophagy degradation phase associated with lysosomal anomalies, thus causing the accumulation of dysfunctional mitochondria labeled by Parkin RBR E3 ubiquitin protein ligase (PARK2)...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28958123/-psychometric-properties-of-the-german-version-of-the-suicide-behaviors-questionnaire-revised-sbq-r
#16
Heide Glaesmer, Nestor D Kapusta, Tobias Teismann, Birgit Wagner, Nina Hallensleben, Lena Spangenberg, Thomas Forkmann
Objectives The Suicide Behaviors Questionnaire-Revised (SBQ-R) was developed by Osman et al. (2001) to economically assess different aspects of suicidality. It consists of 4 items. There are several critical points concerning item construction: (1) temporal frame (e. g. retrospective vs. prospective) and (2) response labels of item 1 and 3 (e. g. 2 alternatives with the same scoring). Information about psychometric properties of the German version of the SBQ-R were not available until now. The SBQ-R is assumed to be a unidimensional measure and is evaluated using a total score, although its dimensionality has never been tested...
September 28, 2017: Psychotherapie, Psychosomatik, Medizinische Psychologie
https://www.readbyqxmd.com/read/28957329/uncovering-the-dna-methylation-landscape-in-key-regulatory-regions-within-the-fads-cluster
#17
Elaheh Rahbar, Hannah C Ainsworth, Timothy D Howard, Gregory A Hawkins, Ingo Ruczinski, Rasika Mathias, Michael C Seeds, Susan Sergeant, James E Hixson, David M Herrington, Carl D Langefeld, Floyd H Chilton
Genetic variants near and within the fatty acid desaturase (FADS) cluster are associated with polyunsaturated fatty acid (PUFA) biosynthesis, levels of several disease biomarkers and risk of human disease. However, determining the functional mechanisms by which these genetic variants impact PUFA levels remains a challenge. Utilizing an Illumina 450K array, we previously reported strong allele-specific methylation (ASM) associations (p = 2.69×10-29) between a single nucleotide polymorphism (SNP) rs174537 and DNA methylation of CpG sites located in the putative enhancer region between FADS1 and FADS2, in human liver tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28938734/hyperspectral-microscopy-can-detect-metabolic-heterogeneity-within-bovine-post-compaction-embryos-incubated-under-two-oxygen-concentrations-7-versus-20
#18
Melanie L Sutton-McDowall, Martin Gosnell, Ayad G Anwer, Melissa White, Malcolm Purdey, Andrew D Abell, Ewa M Goldys, Jeremy G Thompson
STUDY QUESTION: Can we separate embryos cultured under either 7% or 20% oxygen atmospheres by measuring their metabolic heterogeneity? SUMMARY ANSWER: Metabolic heterogeneity and changes in metabolic profiles in morula exposed to two different oxygen concentrations were not detectable using traditional fluorophore and two-channel autofluorescence but were detectable using hyperspectral microscopy. WHAT IS KNOWN ALREADY: Increased genetic and morphological blastomere heterogeneity is associated with compromised developmental competence of embryos and currently forms the basis for embryo scoring within the clinic...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28931595/cell-cycle-transitions-a-common-role-for-stoichiometric-inhibitors
#19
Michael Hopkins, John J Tyson, Béla Novák
The cell division cycle is the process by which eukaryotic cells replicate their chromosomes and partition them to two daughter cells. To maintain the integrity of the genome, proliferating cells must be able to block progression through the division cycle at key transition points (called 'checkpoints'), if there have been problems in the replication of the chromosomes or their biorientation on the mitotic spindle. These checkpoints are governed by protein-interaction networks, composed of phase-specific cell-cycle activators and inhibitors...
September 20, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28929400/can-polymorphisms-in-the-fatty-acid-desaturase-fads-gene-cluster-alter-the-effects-of-fish-oil-supplementation-on-plasma-and-erythrocyte-fatty-acid-profiles-an-exploratory-study
#20
Suzanne J Meldrum, Yuchun Li, Guicheng Zhang, Alexandra E M Heaton, Nina D'Vaz, Judith Manz, Eva Reischl, Berthold V Koletzko, Susan L Prescott, Karen Simmer
PURPOSE: The enzymes encoded by fatty acid desaturases (FADS) genes determine the desaturation of long-chain polyunsaturated fatty acids (LCPUFA). We investigated if haplotype and single nucleotide polymorphisms (SNPs) in FADS gene cluster can influence LCPUFA status in infants who received either fish oil or placebo supplementation. METHODS: Children enrolled in the Infant Fish Oil Supplementation Study (IFOS) were randomly allocated to receive either fish oil or placebo from birth to 6 months of age...
September 19, 2017: European Journal of Nutrition
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