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Decellularization cartilage

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https://www.readbyqxmd.com/read/28660185/dedifferentiated-chondrocytes-in-composite-microfibers-as-tool-for-cartilage-repair
#1
Marco Angelozzi, Letizia Penolazzi, Stefania Mazzitelli, Elisabetta Lambertini, Andrea Lolli, Roberta Piva, Claudio Nastruzzi
Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the composite microfibers has been tested to modulate the behavior and redifferentiation of dedifferentiated chondrocytes...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28634957/bioprinting-of-3d-tissue-models-using-decellularized-extracellular-matrix-bioink
#2
Falguni Pati, Dong-Woo Cho
Bioprinting provides an exciting opportunity to print and pattern all the components that make up a tissue-cells and extracellular matrix (ECM) material-in three dimensions (3D) to generate tissue analogues. A large number of materials have been used for making bioinks; however, majority of them cannot represent the complexity of natural ECM and thus are unable to reconstitute the intrinsic cellular morphologies and functions. We present here a method for making of bioink from decellularized extracellular matrices (dECMs) and a protocol for bioprinting of cell-laden constructs with this novel bioink...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28602577/fabrication-of-porous-scaffolds-with-decellularized-cartilage-matrix-for-tissue-engineering-application
#3
Bita Nasiri, Shohreh Mashayekhan
Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM...
June 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28583182/tissue-specific-bioactivity-of-soluble-tendon-derived-and-cartilage-derived-extracellular-matrices-on-adult-mesenchymal-stem-cells
#4
Benjamin B Rothrauff, Guang Yang, Rocky S Tuan
BACKGROUND: Biological scaffolds composed of tissue-derived extracellular matrix (ECM) can promote homologous (i.e., tissue-specific) cell differentiation through preservation of biophysical and biochemical motifs found in native tissues. Solubilized ECMs derived from decellularized tendon and cartilage have recently been promoted as tissue-specific biomaterials, but whether tissue-specific bioactivity is preserved following solubilization is unknown. This study explored the tissue-specific bioactivity of soluble decellularized tendon and cartilage ECMs on human bone marrow-derived mesenchymal stem cells (MSCs) presented across different culture microenvironments, including two-dimensional (2D) tissue culture plastic, aligned electrospun nanofibers, cell pellets, and cell-seeded photocrosslinkable hydrogels...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28562183/extracellular-matrices-for-bone-regeneration-a-literature-review
#5
Alaa Mansour, Mohamed Amine Mezour, Zahi Badran, Faleh Tamimi
The gold standard material for bone regeneration is still autologous bone, a mesenchymal tissue that consists mainly of extracellular matrix (ECM) (90% v/v) and little cellular content (10% v/v). However, the fact that decellularized allogenic bone grafts often present a clinical performance comparable to autologous bone grafts demonstrates the crucial role of ECM in bone regeneration. For long, the mechanism by which bone allografts function was not clear, but recent research has unveiled many unique characteristics of ECM that seem to play a key role in tissue regeneration...
July 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28546133/tissue-engineered-trachea-regeneration-using-decellularized-trachea-matrix-treated-with-laser-micropore-technique
#6
Yong Xu, Dan Li, Zongqi Yin, Aijuan He, Miaomiao Lin, Gening Jiang, Xiao Song, Xuefei Hu, Yi Liu, Jinpeng Wang, Xiaoyun Wang, Liang Duan, Guangdong Zhou
Tissue-engineered trachea provides a promising approach for reconstruction of long segmental tracheal defects. However, a lack of ideal biodegradable scaffolds greatly restricts its clinical translation. Decellularized trachea matrix (DTM) is considered a proper scaffold for trachea cartilage regeneration owing to natural tubular structure, cartilage matrix components, and biodegradability. However, cell residual and low porosity of DTM easily result in immunogenicity and incomplete cartilage regeneration. To address these problems, a laser micropore technique (LMT) was applied in the current study to modify trachea sample porosity to facilitate decellular treatment and cell ingrowth...
May 22, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28493212/composite-bioscaffolds-incorporating-decellularized-ecm-as-a-cell-instructive-component-within-hydrogels-as-in-vitro-models-and-cell-delivery-systems
#7
Arthi Shridhar, Elizabeth Gillies, Brian G Amsden, Lauren E Flynn
Decellularized tissues represent promising biomaterials, which harness the innate capacity of the tissue-specific extracellular matrix (ECM) to direct cell functions including stem cell proliferation and lineage-specific differentiation. However, bioscaffolds derived exclusively from decellularized ECM offer limited versatility in terms of tuning biomechanical properties, as well as cell-cell and cell-ECM interactions that are important mediators of the cellular response. As an alternative approach, in the current chapter we describe methods for incorporating cryo-milled decellularized tissues as a cell-instructive component within a hydrogel carrier designed to crosslink under mild conditions...
May 11, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28488244/decellularized-iliotibial-band-recolonized-with-allogenic-homotopic-fibroblasts-or-bone-marrow-derived-mesenchymal-stromal-cells
#8
Clemens Gögele, Silke Schwarz, Benjamin Ondruschka, Niels Hammer, Gundula Schulze-Tanzil
Decellularized scaffolds present promising biomimetic approaches in various fields of tissue engineering. Different tissues have been selected for decellularization, among them extracellular matrix (ECM)-rich tissues such as tendons, ligaments and cartilage. The dense ECM of ligaments is particularly challenging to achieve a completely non-immunogenic ECM void of any cells. Here, the methods for decellularization adapted to ligamentous tissue of the iliotibial band (ITB) are presented along with cell isolation and several recolonization techniques using allogenic ITB-derived fibroblasts or mesenchymal stromal cells (MSCs)...
May 10, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28486778/efficacy-of-thermoresponsive-photocrosslinkable-hydrogels-derived-from-decellularized-tendon-and-cartilage-extracellular-matrix-for-cartilage-tissue-engineering
#9
Benjamin B Rothrauff, Luca Coluccino, Riccardo Gottardi, Luca Ceseracciu, Silvia Scaglione, Luca Goldoni, Rocky S Tuan
Tissue engineering using adult mesenchymal stem cells (MSCs), a promising approach for cartilage repair, is highly dependent on the nature of the matrix scaffold. Thermoresponsive, photocrosslinkable hydrogels were fabricated by functionalizing pepsin-soluble decellularized tendon and cartilage extracellular matrices (ECM) with methacrylate groups. Methacrylated gelatin hydrogels served as controls. When seeded with human bone marrow mesenchymal stem cells (MSCs) and cultured in chondrogenic medium, methacrylated ECM hydrogels experienced less cell-mediated contraction, as compared against non-methacrylated ECM hydrogels...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28480504/the-feasibility-of-gamma-radiation-sterilization-for-decellularized-tracheal-grafts
#10
Christopher M Johnson, DeHuang Guo, Stephanie Ryals, Gregory N Postma, Paul M Weinberger
OBJECTIVES/HYPOTHESIS: The most promising stem cell-derived tracheal transplantation approach is dependent upon the use of decellularized tracheal grafts. It has been assumed that a sterilization step, such as gamma radiation, would damage the delicate extracellular matrix of the graft, thus rendering it less viable for cellular repopulation, although this has not been thoroughly investigated. STUDY DESIGN: Laboratory-based comparative analysis. METHODS: Fifteen murine tracheas of strain C57/B-6 mice were obtained...
May 7, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28451995/a-protocol-to-prepare-decellularized-stem-cell-matrix-for-rejuvenation-of-cell-expansion-and-cartilage-regeneration
#11
Jingting Li, Ming Pei
Traditional ex vivo expansion of adult stem cells yields an insufficient quantity of less potent cells. Here we describe the fabrication of decellularized matrix deposited by synovium-derived stem cells (SDSCs). This matrix could serve as a three-dimensional expansion system to rejuvenate cells for proliferation and tissue-specific differentiation potential, which could benefit cartilage regeneration. The decellularized stem cell matrix (DSCM) might be a powerful system for tissue engineering and regeneration...
April 28, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28359676/transplantation-of-the-decellularized-tracheal-allograft-in-animal-model-rabbit
#12
Reza Ershadi, Mohamadbagher Rahim, Siavash Jahany, Siamak Rakei
BACKGROUND: It has been difficult to perform tracheal allotransplantation without immunosuppression. To determine whether decellularized trachea can be used in tracheal replacement, we evaluated the viability of decellularized tracheal allografts in a rabbit model of immunosuppressant-free transplantation. METHOD: Half allograft (Group 1, n = 7) was harvested from adult New Zealand white rabbits, subjected to a detergent-enzymatic method (containing sodium deoxycholate/DNase lavations) of decellularization for as many cycles as needed, and the other half was stored in phosphate-buffered saline at 4°C as a control (Group 2, n = 7)...
March 27, 2017: Asian Journal of Surgery
https://www.readbyqxmd.com/read/28345410/transplantation-of-chemically-processed-decellularized-meniscal-allografts
#13
Kolja Gelse, Ludwig Körber, Martin Schöne, Kay Raum, Peter Koch, Milena Pachowsky, Götz Welsch, Roman Breiter
Objective The aim of this study was to evaluate the chondroprotective effect of chemically decellularized meniscal allografts transplanted into the knee joints of adult merino sheep. Methods Lateral sheep meniscal allografts were chemically processed by a multistep method to yield acellular, sterile grafts. The grafts were transplanted into the knee joints of sheep that were treated by lateral meniscectomy. Joints treated by meniscectomy only and untreated joints served as controls. The joints were analyzed morphologically 6 and 26 weeks after surgery by the macroscopical and histological OARSI (Osteoarthritis Research Society International) score...
April 2017: Cartilage
https://www.readbyqxmd.com/read/28326727/-development-and-characterization-of-oriented-scaffolds-derived-from-cartilage-extracellular-matrix
#14
Li Kun, Zhao Yanhong, Xu Chen, Wang Lianyong, Yang Qiang, Li Hongfa, Teng Binhong
OBJECTIVE: This study aimed to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and to investigate their physicochemical property and compatibility with adipose-derived stem cells (ADSCs). METHODS: A fresh porcine articular cartilage was cut into pieces. Cartilage nanofibers with diameters of 50-500 nm were collected through homogenization and centrifugation. These nanofibers were then decellularized by using Triton X-100 to produce 6% CECM...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28191770/stem-cell-based-tissue-engineered-laryngeal-replacement
#15
Tahera Ansari, Peggy Lange, Aaron Southgate, Karin Greco, Carla Carvalho, Leanne Partington, Anthony Bullock, Sheila MacNeil, Mark W Lowdell, Paul D Sibbons, Martin A Birchall
Patients with laryngeal disorders may have severe morbidity relating to swallowing, vocalization, and respiratory function, for which conventional therapies are suboptimal. A tissue-engineered approach would aim to restore the vocal folds and maintain respiratory function while limiting the extent of scarring in the regenerated tissue. Under Good Laboratory Practice conditions, we decellularized porcine larynges, using detergents and enzymes under negative pressure to produce an acellular scaffold comprising cartilage, muscle, and mucosa...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28169595/tissue-derived-extracellular-matrix-bioscaffolds-emerging-applications-in-cartilage-and-meniscus-repair
#16
Farrah A Monibi, James L Cook
Musculoskeletal injuries are a common problem in orthopedic practice. Given the long-term consequences of unaddressed cartilage and meniscal pathology, a number of treatments have been attempted to stimulate repair or to replace the injured tissue. Despite advances in orthopedic surgery, effective treatments for cartilage and meniscus injuries remain a significant clinical challenge. Tissue engineering is a developing field that aims to regenerate injured tissues with a combination of cells, scaffolds, and signals...
March 7, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28138909/colloidal-gels-with-extracellular-matrix-particles-and-growth-factors-for-bone-regeneration-in-critical-size-rat-calvarial-defects
#17
Jakob M Townsend, S Connor Dennis, Jonathan Whitlow, Yi Feng, Jinxi Wang, Brian Andrews, Randolph J Nudo, Michael S Detamore, Cory J Berkland
Colloidal gels encapsulating natural materials and exhibiting paste-like properties for placement are promising for filling complex geometries in craniofacial bone regeneration applications. Colloidal materials have demonstrated modest clinical outcomes as bone substitutes in orthopedic applications, but limited success in craniofacial applications. As such, development of a novel colloidal gel will fill a void in commercially available products for use in craniofacial reconstruction. One likely application for this technology is cranial reconstruction...
January 30, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28005380/hyaluronic-acid-hydroxyapatite-colloidal-gels-combined-with-micronized-native-ecm-as-potential-bone-defect-fillers
#18
S Connor Dennis, Jonathan Whitlow, Michael S Detamore, Sarah L Kieweg, Cory J Berkland
One of the grand challenges in translational regenerative medicine is the surgical placement of biomaterials. For bone regeneration in particular, malleable and injectable colloidal gelsare frequently designed to exhibit self-assembling and shear-response behavior which facilitates biomaterial placement in tissue defects. The current study demonstrated that by combining native extracellular matrix (ECM) microparticles, i.e., demineralized bone matrix (DBM) and decellularized cartilage (DCC), with hyaluronic acid (HA) and hydroxyapatite (HAP) nanoparticles, a viscoelastic colloidal gel consisting exclusively of natural materials was achieved...
December 22, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27925229/plga-ptmc-cultured-bone-mesenchymal-stem-cell-scaffold-enhances-cartilage-regeneration-in-tissue-engineered-tracheal-transplantation
#19
Bingyang Yan, Zhipei Zhang, Xiaoping Wang, Yunfeng Ni, Yongshi Liu, Tao Liu, Wuping Wang, Hao Xing, Ying Sun, Jian Wang, Xiao-Fei Li
The treatment of long-segment tracheal defect requires the transplantation of effective tracheal substitute, and the tissue-engineered trachea (TET) has been proposed as an ideal tracheal substitute. The major cause of the failure of segmental tracheal defect reconstruction by TET is airway collapse caused by the chondromalacia of TET cartilage. The key to maintain the TET structure is the regeneration of chondrocytes in cartilage, which can secrete plenty of cartilage matrices. To address the problem of the chondromalacia of TET cartilage, this study proposed an improved strategy...
May 2017: Artificial Organs
https://www.readbyqxmd.com/read/27891678/morphomechanics-of-dermis-a-method-for-non-destructive-testing-of-collagenous-tissues
#20
R G Shah, M C Pierce, F H Silver
BACKGROUND: Collagenous tissues store, transmit and dissipate elastic energy during mechanical deformation. In skin, mechanical energy is stored during loading and then is dissipated, which protects skin from mechanical failure. Thus, energy storage (elastic properties) and dissipation (viscous properties) are important characteristics of extracellular matrices (ECMs) that support the cyclic loading of ECMs without tissue failure. METHODS: Uniaxial stress-strain measurements on decellularized human dermis have been made and compared to results of a non-destructive technique involving optical coherence tomography (OCT) combined with vibrational analysis...
November 27, 2016: Skin Research and Technology
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