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Decellularization cartilage

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https://www.readbyqxmd.com/read/29449572/optimising-the-decellularization-of-human-elastic-cartilage-with-trypsin-for-future-use-in-ear-reconstruction
#1
Shafiq Rahman, Michelle Griffin, Anish Naik, Matthew Szarko, Peter E M Butler
Decellularized scaffolds can induce chondrogenic differentiation of stem cells. This study compares different methods to optimise the decellularization of auricular cartilage. The process consisted of an initial 12 hour dry freeze thaw which froze the cartilage specimens in an empty tube at -20 °C. Samples were allowed to thaw at room temperature followed by submersion in phosphate buffer solution in which they were frozen at -20 °C for a 12 hour period. They were then allowed to thaw at room temperature as before...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29427545/evaluation-of-decellularized-xenogenic-porcine-auricular-cartilage-as-a-novel-biocompatible-filler
#2
Sung-Chan Shin, Hee Young Park, Nari Shin, Da-Woon Jung, Hyun-Keun Kwon, Ji Min Kim, Soo-Geun Wang, Jin-Choon Lee, Eui-Suk Sung, Gi Cheol Park, Byung-Joo Lee
Fillers are products that fill the space in soft tissues of the human body and actively used in the various medical fields. Unfortunately, most of the cost-effective commercially available fillers are synthetic and have limitations in terms of their biocompatibility. Here, we evaluated the possible application of decellularized xenogenic cartilage as a long-lasting material for soft tissue augmentation and compared it with two commercially available fillers Artesense (polymethylmethacrylate microspheres) and Radiesse (calcium hydroxyapatite [CaHa])...
February 10, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29423248/capsular-augmentation-in-colonna-arthroplasty-for-the-management-of-chronic-hip-dislocation
#3
Matthew Sloan, Atul F Kamath
Colonna capsular arthroplasty represents an option for the management of chronic hip dislocation in young patients with dysplasia. In the appropriate patient, modern capsular arthroplasty procedures may provide an opportunity for hip preservation in patients with preserved femoral head cartilage and not appropriate for total hip arthroplasty. Here, we review our experience with surgical dislocation of the hip and capsular arthroplasty in a 27-year-old female with congenital hip dysplasia and chronic superior hip dislocation...
January 2018: Journal of Hip Preservation Surgery
https://www.readbyqxmd.com/read/29411714/effects-of-tissue-processing-on-bioactivity-of-cartilage-matrix-based-hydrogels-encapsulating-osteoconductive-particles
#4
Jakob M Townsend, Taylor A Zabel, Yi Feng, Jinxi Wang, Brian T Andrews, Randy Nudo, Cory Berkland, Michael S Detamore
In the treatment of severe traumatic brain injury (TBI), decompressive craniectomy is commonly used to remove a large portion of calvarial bone to allow unimpeded brain swelling. Hydrogels have the potential to revolutionize TBI treatment by permitting a single-surgical intervention, remaining pliable during brain swelling, and tuned to regenerate bone after swelling has subsided. With this motivation, our goal is to present a pliable material capable of regenerating calvarial bone across a critical size defect...
February 7, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29394158/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-in-vitro-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#5
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick W Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS + dCM was 63.4%. The sustained release of proteins from MS + dCM was observed over 4 weeks in vitro...
February 2, 2018: Biofabrication
https://www.readbyqxmd.com/read/29370745/design-and-fabrication-of-injectable-microcarriers-composed-of-acellular-cartilage-matrix-and-chitosan
#6
Farzane Sivandzade, Shohreh Mashayekhan
Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellularextracellular matrix (ECM) of cartilage tissue and chitosan(CS), with different ECM weight ratios, were fabricated by electrosprayingtechnique to be used in the treatment of articular cartilage defects...
January 26, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29317369/mesenchymal-stem-cell-derived-extracellular-matrix-enhances-chondrogenic-phenotype-of-and-cartilage-formation-by-encapsulated-chondrocytes-in-vitro-and-in-vivo
#7
Yuanheng Yang, Hang Lin, He Shen, Bing Wang, Guanghua Lei, Rocky S Tuan
Mesenchymal stem cell derived extracellular matrix (MSC-ECM) is a natural biomaterial with robust bioactivity and good biocompatibility, and has been studied as a scaffold for tissue engineering. In this investigation, we tested the applicability of using decellularized human bone marrow derived MSC-ECM (hBMSC-ECM) as a culture substrate for chondrocyte expansion in vitro, as well as a scaffold for chondrocyte-based cartilage repair. hBMSC-ECM deposited by hBMSCs cultured on tissue culture plastic (TCP) was harvested, and then subjected to a decellularization process to remove hBMSCs...
January 6, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29313825/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-i-in-vitro-i-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#8
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS+dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS+dCM was 63.4%. The sustained release of proteins from MS+dCM was observed over 4 weeks in vitro. Both MS+dCM and MS(-) were cytocompatible...
January 9, 2018: Biofabrication
https://www.readbyqxmd.com/read/29287161/evaluation-of-equine-synovial-derived-extracellular-matrix-scaffolds-seeded-with-equine-synovial-derived-mesenchymal-stem-cells
#9
Nathalie A Reisbig, Hayam A Hussein, Erin Pinnell, Alicia L Bertone
OBJECTIVE To create a bioactive synovium scaffold by infusing decellularized synovial-derived extracellular matrix (synECM) with synovial-derived mesenchymal stem cells (synMSCs). SAMPLE Synovium from the femoropatellar and medial femorotibial joints of equine cadavers. PROCEDURES The synMSCs were cultured in monolayer and not treated or cotransduced to enhance expression of green fluorescent protein (GFP) and human bone morphogenetic protein (BMP)-2. The synECM was decellularized with 0.1% peracetic acid and then seeded with synMSCs (0...
January 2018: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29233641/biphasic-hierarchical-extracellular-matrix-scaffold-for-osteochondral-defect-regeneration
#10
Xianfeng Lin, Jiaxin Chen, Pengcheng Qiu, Qi Zhang, Shengyu Wang, Mingzhen Su, Yunbin Chen, Keke Jin, An Qin, Shunwu Fan, Pengfei Chen, Xing Zhao
OBJECTIVE: To investigate the effect of decellularized osteochondral extracellular matrix (ECM) scaffold for osteochondral defect regeneration. DESIGN: We compared the histological features and microstructure of degenerated cartilage to normal articular cartilage. We also generated and evaluated osteochondral ECM scaffolds through decellularization technology. Then scaffolds were implanted to osteochondral defect in rabbit model. After 12 weeks surgery, regeneration tissues were analyzed by histology, immunohistochemistry evaluation...
December 9, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29223730/characterization-of-a-new-decellularized-bovine-pericardial-biological-mesh-structural-and-mechanical-properties
#11
Alessandra Bielli, Roberta Bernardini, Dimitrios Varvaras, Piero Rossi, Giancarlo Di Blasi, Giuseppe Petrella, Oreste Claudio Buonomo, Maurizio Mattei, Augusto Orlandi
Implants made from naturally-derived biomaterials, also called biological meshes or biomeshes, typically derive from decellularized extracellular matrix of either animal or human tissue. Biomeshes have many biomedical applications such as ligament repair, bone and cartilage regeneration and soft tissue replacement. Bovine collagen is one of the most widely used and abundantly available xenogenic materials. In particular, bovine pericardium is widely used as extracellular matrix bioprosthetic tissue. The efficiency of a pericardial mesh to function as scaffold depends on the quality of the decellularization protocol used...
February 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29210638/medical-applications-of-collagen-and-collagen-based-materials
#12
Anatoly Borisovich Shekhter, Alexey Leonidovich Fayzullin, Marina Nikolaevna Vukolova, Tatyana Georgiyevna Rudenko, Varvara Dmitriyevna Osipycheva, Petr Francevich Litvitsky
Collagen and collagen-based materials have been successfully used in medicine for over 50 years. The number of scientific articles about the role of collagen in the construction of scaffolds for tissue engineering has risen precipitously in recent years. The review contains materials about historic and modern applications of collagen in medicine such as soluble collagen injections, solid constructs reconstructed from solution, and decellularized collagen matrices. The analysis of published data proves the efficacy of collagen material in the treatment of chronic wounds, burns, venous and diabetic ulcers, in plastic, reconstructive and general surgery, urology, proctology, gynecology, ophthalmology, otolaryngology, neurosurgery, dentistry, cardiovascular and bone and cartilage surgery, as well as in cosmetology...
December 5, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29209868/laser-surface-modification-of-decellularized-extracellular-cartilage-matrix-for-cartilage-tissue-engineering
#13
Eva Goldberg-Bockhorn, Silke Schwarz, Rachana Subedi, Alexander Elsässer, Ricarda Riepl, Paul Walther, Ludwig Körber, Roman Breiter, Karl Stock, Nicole Rotter
The implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by carbon dioxide (CO2) and Er:YAG laser application to facilitate the migration of chondrocytes inside the scaffold...
December 6, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28967543/high-short-term-failure-rate-associated-with-decellularized-osteochondral-allograft-for-treatment-of-knee-cartilage-lesions
#14
Christine C Johnson, Daniel J Johnson, Grant H Garcia, Dean Wang, Mollyann Pais, Ryan M Degen, Alissa J Burge, Riley J Williams
PURPOSE: To report the short-term clinical and radiographic outcomes following the use of decellularized osteochondral (OC) allograft plugs in the treatment of distal femoral OC lesions. METHODS: An Institutional Review Board-approved database with prospectively collected data was used to identify patients treated with the decellularized OC allograft plugs implant. Demographic information, patient-reported outcomes, magnetic resonance imaging (MRI), and the number and type of reoperations were assessed...
September 26, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28967085/tracheal-decellularization-using-a-combination-of-chemical-physical-and-bioreactor-methods
#15
Yourka D Tchoukalova, Justin M Hintze, Richard E Hayden, David G Lott
INTRODUCTION: The purpose of this study was to compare different decellularization protocols with the conventional detergent enzymatic method (DEM) using continuous agitation. METHODS: The first experiment compared conventional DEM with sonication and lyophilization+freeze-thaw cycles. A second experiment was carried out to compare time-adjusted DEM (2-hour instead of 4-hour incubations with 4% deoxycholate) to decellularization in a bioreactor. Cellularity was determined by DNA-quantitation, H&E-staining and immunostaining for major histocompatibility complex-1 (MHC-1)...
September 28, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28946629/suitability-of-egcg-as-a-means-of-stabilizing-a-porcine-osteochondral-xenograft
#16
Steven Elder, John Clune, Jaylyn Walker, Paul Gloth
As a non-crosslinked osteochondral xenograft would be mechanically inferior to native cartilage and vulnerable to premature degradation, we seek a safe and effective method of xenograft stabilization. The purpose of this study was to evaluate the capacity for epigallocatechin gallate (EGCG) to stabilize a decellularized porcine osteochondral xenograft through collagen crosslinking. Our objectives were to assess the effects of EGCG on the degree of crosslinking, mechanical properties, collagenase resistance, cytotoxicity, and in vitro biocompatibility...
September 23, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28932116/silk-fibroin-cartilage-extracellular-matrix-scaffolds-with-sequential-delivery-of-tgf-%C3%AE-3-for-chondrogenic-differentiation-of-adipose-derived-stem-cells
#17
Qiang Yang, Bin-Hong Teng, Li-Na Wang, Kun Li, Chen Xu, Xin-Long Ma, Yang Zhang, De-Ling Kong, Lian-Yong Wang, Yan-Hong Zhao
A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28922982/porcine-mesothelium-wrapped-diced-cartilage-grafts-for-nasal-reconstruction
#18
Athanasios Bramos, David P Perrault, Alexander N Fedenko, Gene H Kim, Sofia Bougioukli, Jay R Lieberman, Jay W Calvert, Alex K Wong
BACKGROUND: Fascia-wrapped diced cartilage grafts have become a useful tool in modern rhinoplasty surgery. Unfortunately, fascial harvest is associated with donor site morbidity; therefore, a nonautologous alternative to fascia would be ideal. Decellularized porcine mesothelium (PM), Meso BioMatrix™, is an acellular scaffold that could potentially fill this need. To determine if PM could serve as an acceptable alternative, we histologically compared diced cartilage grafts wrapped in fascia versus PM...
January 5, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28922323/face-graft-scaffold-production-in-a-rat-model
#19
Jérôme Duisit, Hadrien Amiel, Giuseppe Orlando, Adeline Dedriche, Catherine Behets, Pierre Gianello, Benoît Lengelé
BACKGROUND: As a route towards face bioengineering, our group previously reported the production of a complete scaffold production by perfusion-decellularization of a porcine ear subunit graft and partial recellularization. In order to extend the scaffold to the whole face and to down-scale it, we applied our findings to a rodent hemi-facial graft model. METHODS: After euthanasia, seven full-thickness rat hemi-face grafts were harvested with the CCA and the EJV as pedicle, and cannulated...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28879659/extracellular-matrix-particle-glycosaminoglycan-composite-hydrogels-for-regenerative-medicine-applications
#20
Vince Beachley, Garret Ma, Chris Papadimitriou, Matt Gibson, Michael Corvelli, Jennifer Elisseeff
Tissue extracellular matrix (ECM) is a complex material made up of fibrous proteins and ground substance (glycosaminoglycans, GAGs) that are secreted by cells. ECM contains important biological cues that modulate cell behaviors, and it also serves as a structural scaffold to which cells can adhere. For clinical applications, where immune rejection is a constraint, ECM can be processed using decellularization methods intended to remove cells and donor antigens from tissue or organs, while preserving native biological cues essential for cell growth and differentiation...
September 6, 2017: Journal of Biomedical Materials Research. Part A
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