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Glycosylated Albumin

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https://www.readbyqxmd.com/read/29035939/predictors-for-the-development-of-microalbuminuria-and-interaction-with-renal-function
#1
Christos Chatzikyrkou, Jan Menne, Joseph Izzo, Giancarlo Viberti, Ton Rabelink, Luis M Ruilope, Christian Rump, Peter R Mertens, Hermann Haller
AIM: It is important to know which factors predict the development of microalbuminuria in patients with diabetes mellitus type II. MATERIAL: Data from the Randomized Olmesartan and Diabetes Microalbuminuria Prevention Study were used to identify predictors for the new onset of microalbuminuria. Furthermore, the interaction of baseline albuminuria and baseline estimated glomerular filtration rate (eGFR) and the effects of treatment with olmesartan were investigated...
October 13, 2017: Journal of Hypertension
https://www.readbyqxmd.com/read/28976746/tailored-multivalent-neo-glycoproteins-synthesis-evaluation-and-application-of-a-library-of-galectin-3-binding-glycan-ligands
#2
Dominic Laaf, Pavla Bojarová, Helena Pelantová, Vladimír Křen, Lothar Elling
Galectin-3 (Gal-3), a member of the β-galactoside-binding lectin family, is a tumor biomarker and involved in tumor angiogenesis and metastasis. Gal-3 is therefore considered as a promising target for early cancer diagnosis and anticancer therapy. We here present the synthesis of a library of tailored multivalent neo-glycoproteins and evaluate their Gal-3 binding properties. By the combinatorial use of glycosyltransferases and chemo-enzymatic reactions, we first synthesized a set of N-acetyllactosamine (Galβ1,4GlcNAc; LacNAc type 2)-based oligosaccharides featuring five different terminating glycosylation epitopes, respectively...
October 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28954262/protective-effects-of-adiponectin-against-diabetic-renal-injury-in-a-mouse-model-of-diabetes
#3
Jiacai Hu, Junjun Dong, Zhijie Yang, Hao Wu, Na Yang
BACKGROUND/AIMS: Adiponectin (Apn) has shown anti-diabetic and anti-inflammatory potential. In the study, we studied and tested the protective effects of Apn against diabetic renal injury and the possible mechanism of these effects. METHODS: After 1 week of adaptive feeding, 30 mice were randomly divided into 5 groups: the control group, the model group, the Apn (L) group, the Apn (M) group and the Apn (H) group. All mice were marked and weighed. Following 4 weeks of a pro-diabetic high-fat diet, the model group and Apn groups were injected intraperitoneally with a high dose of STZ (85 mg/kg), while the normal control group was injected with sodium citrate...
September 28, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28934288/purification-of-rabbit-serum-histidine-proline-rich-glycoprotein-via-preparative-gel-electrophoresis-and-characterization-of-its-glycosylation-patterns
#4
Anna Katharina Weyrauch, Mario Jakob, Angelika Schierhorn, Ralf Bernd Klösgen, Dariush Hinderberger
Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role has not been discovered yet. Past purification approaches out of plasma or serum often led to co-purification of other proteins so that for a profound understanding of the function it is important to obtain a protein of high purity. Recent purification strategies were based upon metale chelate affinity chromatography followed by anion exchange chromatography or size exclusion chromatography, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28932995/pharmacokinetics-and-preliminary-pharmacodynamics-of-single-and-multiple-dose-lyophilized-recombinant-glucagon-like-peptide-1-receptor-agonist-re-4-in-chinese-patients-with-type-2-diabetes-mellitus
#5
Yitong Wang, Bingfeng Xu, Lixia Zhu, Kun Lou, Yingli Chen, Xia Zhao, Qian Wang, Ling Xu, Xiaohui Guo, Linong Ji, Yimin Cui, Yi Fang
BACKGROUND AND OBJECTIVES: Recombinant glucagon-like peptide-1 receptor agonist (rE-4) is a glucagon-like peptide-1 receptor agonist, which has the same amino acid sequence to exenatide, except for the C-terminal deamidated. This study assessed the pharmacokinetics and preliminary pharmacodynamics of rE-4, following single and multiple subcutaneous injections in Chinese patients with type 2 diabetes mellitus (T2DM). DESIGN AND METHODS: In the randomized, open-label study, Chinese patients with T2DM (n = 36) were randomly assigned to three groups of rE-4 (n = 12), rE-4 with metformin (n = 12) and exenatide (n = 12, as the control group) for 12 weeks...
September 20, 2017: Clinical Drug Investigation
https://www.readbyqxmd.com/read/28932884/facile-and-easily-popularized-synthesis-of-l-cysteine-functionalized-magnetic-nanoparticles-based-on-one-step-functionalization-for-highly-efficient-enrichment-of-glycopeptides
#6
Xiaoyan Feng, Chunhui Deng, Mingxia Gao, Xiangmin Zhang
Protein glycosylation is one of the most important post-translational modifications. Also, efficient enrichment and separation of glycopeptides from complex samples are crucial for the thorough analysis of glycosylation. Developing novel hydrophilic materials with facile and easily popularized synthesis is an urgent need in large-scale glycoproteomics research. Herein, for the first time, a one-step functionalization strategy based on metal-organic coordination was proposed and Fe3O4 nanoparticles were directly functionalized with zwitterionic hydrophilic L-cysteine (L-Cys), greatly simplifying the synthetic procedure...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28920905/electrochemical-impedance-spectroscopy-on-interdigitated-gold-microelectrodes-for-glycosylated-human-serum-albumin-characterization
#7
Nadra Bohli, Hanen Chammem, Olivier Meilhac, Laurence Mora, Adnane Abdelghani
Glycosylated albumin is considered as a potentially accurate indicator of shorter-term average glucose concentration compared to the current standard HbA1c and as such, it's attracting the interest of the scientific community as a possible diagnosis marker for diabetic patients. The purpose of this paper is to achieve a better understanding of the glycation effect of albumin on its electrochemical properties. That is done through the use of Interdigitated gold microelectrodes (IDGE) as support in a label free impedimetric immunosensor for detection of human serum albumin detection in glycated (GA) and non-glycated (HSA) form...
September 14, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28900186/improvement-of-the-glycoproteomic-toolbox-with-the-discovery-of-a-unique-c-terminal-cleavage-specificity-of-flavastacin-for-n-glycosylated-asparagine
#8
Alexander Pralow, Marcus Hoffmann, Terry Nguyen-Khuong, Erdmann Rapp, Udo Reichl
To determine all potential N-glycosylation sites of a glycoprotein, one central aspect of every bottom-up N-glycoproteomic strategy is to generate suitable N-glycopeptides that can be detected and analyzed by mass spectrometry. Specific proteases, such as trypsin, bear the potential of generating N-glycopeptides that either carry more than one N-glycosylation site or are too long to be readily analyzed by mass spectrometry- both due to the lack of tryptic cleavage sites near the N-glycosylation site. Here, we present a newly identified cleavage specificity of flavastacin, a protease from Flavobacterium menigosepticum, which - up to now - was only reported to cleave peptide bonds N-terminal to aspartic acid residues...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891998/bioactivity-in-vitro-of-quercetin-glycoside-obtained-in-beauveria-bassiana-culture-and-its-interaction-with-liposome-membranes
#9
Paulina Strugała, Tomasz Tronina, Ewa Huszcza, Janina Gabrielska
Quercetin (Q) was used as substrate for regioselective glycosylation at the C-7 position catalyzed by Beauveria bassiana AM278 strain. As a result the glycoside quercetin 7-O-β-d-(4″-O-methyl)glucopyranoside (Q 7-MeGlu) was formed. The goal of the studies was to determine the anti-oxidative (liposome membrane protection against free radicals IC50(Q 7-MeGlu) = 5.47 and IC50(Q) = 4.49 µM) and anti-inflammatory (COX-1 and COX-2 enzymes activity inhibition) properties of Q 7-MeGlu as compared to Q. Every attempt was made to clarify the antioxidant activity of these molecules, which are able to interact with egg phosphatidylcholine liposomes, using a fluorometric method (by applying the probes MC540, TMA-DPH and DPH)...
September 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28843828/vinpocetine-mitigates-proteinuria-and-podocytes-injury-in-a-rat-model-of-diabetic-nephropathy
#10
Walaa Wadie, Dalia M El-Tanbouly
Podocyte injury and glomerular basement membrane thickening have been considered as essential pathophysiological events in diabetic nephropathy. The aim of this study was to investigate the possible beneficial effects of vinpocetine on diabetes-associated renal damage. Male Wistar rats were made diabetic by injection of streptozotocin (STZ). Diabetic rats were treated with vinpocetine in a dose of 20mg/kg/day for 6 weeks. Treatment with vinpocetine resulted in a marked decrease in the levels of blood glucose, glycosylated haemoglobin, creatinine, blood urea nitrogen, urinary albumin and albumin/creatinine ratio along with an elevation in creatinine clearance rate...
November 5, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28839380/optimization-of-polysaccharide-ultrasonic-extraction-conditions-using-purple-sweet-potato-tubers-based-on-free-radical-scavenging-and-glycosylation-inhibitory-bioactivities
#11
Haihua Guo, Fansheng Kong, Chunyan Yan
BACKGROUND: The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE: To predict the ultrasonic conditions for getting the optimal in vitro antioxidant and antiglycated activity of ultrasonic extracted polysaccharides from purple sweet potato (I. batatas) tubers, the artificial neural network (ANN) regression models was used in this study...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28796164/enzymatic-synthesis-of-n-acetyllactosamine-lacnac-type-1-oligomers-and-characterization-as-multivalent-galectin-ligands
#12
Thomas Fischöder, Dominic Laaf, Carina Dey, Lothar Elling
Repeats of the disaccharide unit N-acetyllactosamine (LacNAc) occur as type 1 (Galβ1, 3GlcNAc) and type 2 (Galβ1, 4GlcNAc) glycosylation motifs on glycoproteins and glycolipids. The LacNAc motif acts as binding ligand for lectins and is involved in many biological recognition events. To the best of our knowledge, we present, for the first time, the synthesis of LacNAc type 1 oligomers using recombinant β1,3-galactosyltransferase from Escherichia coli and β1,3-N-acetylglucosaminyltranferase from Helicobacter pylori...
August 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28774662/hamilton-rating-scale-for-depression-24-ham-d24-as-a-novel-predictor-for-diabetic-microvascular-complications-in-type-2-diabetes-mellitus-patients
#13
Shuo Pan, Zhong-Wei Liu, Shuang Shi, Xun Ma, Wen-Qian Song, Gong-Chang Guan, Yong Zhang, Shun-Ming Zhu, Fu-Qiang Liu, Bo Liu, Zhi-Guo Tang, Jun-Kui Wang, Ying Lv
The study was designed to investigate whether the hamilton rating scale for depression (24-items) (HAM-D24) can be used to predict the diabetic microvascular complications in type 2 diabetes mellitus (T2DM) patients. 288 hospitalized patients with T2DM were enrolled. Their diabetic microvascular complications including diabetic nephropathy, diabetic retinopathy, diabetic peripheral neuropathy and diabetic foot as well as demographic, clinical data, blood samples and echocardiography were documented. All the enrolled patients received HAM-D24 evaluation...
July 26, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28754033/the-influence-of-glycosylation-of-natural-and-synthetic-prenylated-flavonoids-on-binding-to-human-serum-albumin-and-inhibition-of-cyclooxygenases-cox-1-and-cox-2
#14
Tomasz Tronina, Paulina Strugała, Jarosław Popłoński, Aleksandra Włoch, Sandra Sordon, Agnieszka Bartmańska, Ewa Huszcza
The synthesis of different classes of prenylated aglycones (α,β-dihydroxanthohumol (2) and (Z)-6,4'-dihydroxy-4-methoxy-7-prenylaurone (3)) was performed in one step reactions from xanthohumol (1)-major prenylated chalcone naturally occurring in hops. Obtained flavonoids (2-3) and xanthohumol (1) were used as substrates for regioselective fungal glycosylation catalyzed by two Absidia species and Beauveria bassiana. As a result six glycosides (4-9) were formed, of which four glycosides (6-9) have not been published so far...
July 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28649731/protein-glycan-quinary-interactions-in-crowding-environment-unveiled-by-nmr-spectroscopy
#15
Ana Diniz, Jorge S Dias, Jesús Jiménez-Barbero, Filipa Marcelo, Eurico J Cabrita
Protein-glycan interactions as modulators for quinary structures in crowding environments were explored. The interaction between human galectin 3 (Gal-3) and distinct macromolecular crowders, such as bovine and human serum albumin (BSA and HSA), Ficoll 70 and PEG3350, was scrutinized. The molecular recognition event of the specific ligand, lactose, by Gal-3 in crowding conditions was evaluated. Gal-3 interactions were monitored by NMR analysing chemical shift perturbation (CSP) and line broadening of (1) H(15) N-HSQC signals...
September 21, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28644391/molecular-structure-affinity-relationship-of-flavonoids-in-lotus-leaf-nelumbo-nucifera-gaertn-on-binding-to-human-serum-albumin-and-bovine-serum-albumin-by-spectroscopic-method
#16
Xiaosheng Tang, Ping Tang, Liangliang Liu
Lotus leaf has gained growing popularity as an ingredient in herbal formulations due to its various activities. As main functional components of lotus leaf, the difference in structure of flavonoids affected their binding properties and activities. In this paper, the existence of 11 flavonoids in lotus leaf extract was confirmed by High Performance Liquid Chromatography (HPLC) analysis and 11 flavonoids showed various contents in lotus leaf. The interactions between lotus leaf extract and two kinds of serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA)) were investigated by spectroscopic methods...
June 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28624473/antibody-blood-brain-barrier-efflux-is-modulated-by-glycan-modification
#17
John M Finke, Kari R Ayres, Ryan P Brisbin, Hali A Hill, Emily E Wing, William A Banks
BACKGROUND: Drug delivery to the brain is a major roadblock to treatment of Alzheimer's disease. Recent results of the PRIME study indicate that increasing brain penetration of antibody drugs improves Alzheimer's treatment outcomes. New approaches are needed to better accomplish this goal. Based on prior evidence, the hypothesis that glycan modification alters antibody blood-brain barrier permeability was tested here. METHODS: The blood-brain barrier permeability coefficient Pe of different glycosylated states of anti-amyloid IgG was measured using in vitro models of brain microvascular endothelial cells...
September 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28603992/glycosylated-platinum-iv-complexes-as-substrates-for-glucose-transporters-gluts-and-organic-cation-transporters-octs-exhibited-cancer-targeting-and-human-serum-albumin-binding-properties-for-drug-delivery
#18
Jing Ma, Qingpeng Wang, Zhonglv Huang, Xiande Yang, Quandeng Nie, Wenpei Hao, Peng George Wang, Xin Wang
Glycosylated platinum(IV) complexes were synthesized as substrates for GLUTs and OCTs for the first time, and the cytotoxicity and detailed mechanism were determined in vitro and in vivo. Galactoside Pt(IV), glucoside Pt(IV), and mannoside Pt(IV) were highly cytotoxic and showed specific cancer-targeting properties in vitro and in vivo. Glycosylated platinum(IV) complexes 5, 6, 7, and 8 (IC50 0.24-3.97 μM) had better antitumor activity of nearly 166-fold higher than the positive controls cisplatin (1a), oxaliplatin (3a), and satraplatin (5a)...
July 13, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28578411/red-cell-distribution-width-as-a-significant-indicator-of-medication-and-prognosis-in-type-2-diabetic-patients
#19
Xiao-Fen Xiong, Yuan Yang, Xianghui Chen, Xuejing Zhu, Chun Hu, Yachun Han, Li Zhao, Fuyou Liu, Lin Sun
Whether red cell distribution width (RDW) can be a potential indicator for diabetic nephropathy (DN) is unknown. A total of 809 type 2 diabetes mellitus (T2D) patients were divided into 4 groups according to the quartiles (Q) of the RDW (%): Q1 ≤ 12.4 (n = 229), 12.4 < Q2 ≤ 12.9 (n = 202), 12.9 < Q3 < 13.5 (n = 168), Q4 ≥ 13.5 (n = 210). Results showed that the levels in Q4 group was higher in age, disease duration, systolic blood pressure, blood urea nitrogen, creatinine, uric acid and proteinuria but lower in hemoglobin, serum albumin and glycosylated hemoglobin compared to Q1 group...
June 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28554385/co-overexpression-of-mgat1-and-mgat4-in-cho-cells-for-production-of-highly-sialylated-albumin-erythropoietin
#20
Hyun-Myoung Cha, Jin-Hyuk Lim, Jung-Heum Yeon, Jeong-Min Hwang, Dong-Il Kim
Terminal sialic acids on N-glycan of recombinant human erythropoietin are very important for in vivo half-life, as this glycoprotein has three N-glycosylation sites. N-acetylglucosaminyltransferases I, II, IV, and V (i.e. Mgat1, Mgat2, Mgat4, and Mgat5) catalyze the formation of a glycan antennary structure. These enzymes display different reaction kinetics for a common substrate and generally show low expression in Chinese hamster ovary (CHO) cells. Therefore, genetic control of Mgat expression is an effective method to increase sialic acid contents by enhancing glycan antennarity...
August 2017: Enzyme and Microbial Technology
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