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Glycosylated Albumin

Ying Li, Gao Yang, Jinwei Qiang, Songqi Cai, Hao Zhou
OBJECTIVE: To investigate the incidence of insulin resistance (IR) and diabetes in patients with chronic hepatic schistosomiasis japonica (HSJ) and portosystemic shunts (PSS). METHODS: Pre- and post-contrasted computed tomography images obtained from patients with HSJ and control subjects were reviewed by two radiologists who identified and graded any shunting vessels. Anthropometric measurements, hepatic enzymes, lipid profile, blood levels of albumin, glucose, insulin and homeostasis model assessment (HOMA-2) index of all participants were also assessed...
September 29, 2016: Journal of International Medical Research
Petra Darebna, Petr Novak, Radek Kucera, Ondrej Topolcan, Miloslav Sanda, Radoslav Goldman, Petr Pompach
: Alternations in the glycosylation of proteins have been described in connection with several cancers, including hepatocellular carcinoma (HCC) and colorectal cancer. Analytical tools, which use combination of liquid chromatography and mass spectrometry, allow precise and sensitive description of these changes. In this study, we use MRM and FT-ICR operating in full-MS scan, to determine ratios of intensities of specific glycopeptides in HCC, colorectal cancer, and liver metastasis of colorectal cancer...
September 16, 2016: Journal of Proteomics
Paulina Strugała, Anna Dudra, Janina Gabrielska
We investigated the effects of acylated cyanidin-3-O-β-(6″-O-E-p-coumaroyl-sambubioside)-5-O-β-glucoside (C3-cs-5G) and nonacylated cyanidin, cyanidin-3,5-di-O-β-glucoside (C3,5G) and cyanidin-3-O-β-glucoside (C3G), on cell-mimic membranes (MM) that reflected the membrane lipid composition of tumor cells. The relationship between structural derivatives of cyanidin (Cy-d), membrane interactivity, their antioxidant activity, and interaction with albumin were characterized. Studies showed that Cy-d caused an increase in packing order mainly in the hydrophilic region of the membranes...
October 5, 2016: Journal of Agricultural and Food Chemistry
Michael W Traxlmayr, Jonathan D Kiefer, Raja R Srinivas, Elisabeth Lobner, Alison W Tisdale, Naveen K Mehta, Nicole J Yang, Bruce Tidor, K Dane Wittrup
The Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus is an attractive binding scaffold due to its small size (7 kDa), high thermal stability (Tm of 98degC) and absence of cysteines and glycosylation sites. However, as a DNA-binding protein Sso7d is highly positively charged, introducing a strong specificity constraint for binding epitopes and leading to non-specific interaction with mammalian cell membranes. In the present study, we report charge-neutralized variants of Sso7d that maintain high thermal stability...
August 30, 2016: Journal of Biological Chemistry
Ying Zhang, Ye Peng, Zhichao Bin, Huijie Wang, Haojie Lu
N-linked protein glycosylation is involved in regulation of a wide variety of cellular processes and associated with numerous diseases. Highly specific identification of N-glycome remains a challenge while its biological significance is acknowledged. The relatively low abundance of glycan in complex biological mixtures, lack of basic sites for protonation, and suppression by other highly abundant proteins/peptides lead to the particularly poor detection sensitivity of N-glycans in the MS analysis. Therefore, the highly specific purification procedure becomes a crucial step prior to MS analysis of the N-glycome...
August 31, 2016: Analytica Chimica Acta
Guangyan Qing, Xiuling Li, Peng Xiong, Cheng Chen, Mimi Zhan, Xinmiao Liang, Taolei Sun
Glycoproteomics identifies and catalogs protein glycosylation and explores its impact on protein conformations and biofunctions. However, these studies are restricted by the bottleneck to enrich low-abundance glycopeptides from complex biosamples and the difficulties in analyzing glycan structures by mass spectrometry. Here, we report dipeptide as a simple but promising carbohydrate binding platform to tackle these problems. We build a hydropathy-index-based strategy for sequence optimization and screen out three optimal dipeptide sequences from 54 types of dipeptides...
August 31, 2016: ACS Applied Materials & Interfaces
Md Kamal Hossain, Katherine A Wall
Aberrantly glycosylated mucin 1 (MUC1) is a recognized tumor-specific antigen on epithelial cell tumors. A wide variety of MUC1 glycopeptide anti-cancer vaccines have been formulated by many research groups. Some researchers have used MUC1 alone as an immunogen whereas other groups used different antigenic carrier proteins such as bovine serum albumin or keyhole limpet hemocyanin for conjugation with MUC1 glycopeptide. A variety of adjuvants have been used with MUC1 glycopeptides to improve their immunogenicity...
July 26, 2016: Vaccines
Funmilola Clara Thomas, William Mullen, Riccardo Tassi, Adela Ramírez-Torres, Manikhandan Mudaliar, Tom N McNeilly, Ruth N Zadoks, Richard Burchmore, P David Eckersall
A peptidomic investigation of milk from an experimental model of Streptococcus uberis mastitis in dairy cows has incorporated a study of milk high abundance and acute phase (APP) proteins as well as analysis of low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. uberis caused a shift in abundance from caseins, β-lactoglobulin and α-lactalbumin to albumin, lactoferrin and IgG with the increase in lactoferrin occurring last. The APP response of haptoglobin, mammary associated serum amyloid A3 and C-reactive protein occurred between 30-48 hours post challenge with peak concentrations of APPs at 72-96 hours post challenge and declined thereafter at a rate resembling the fall in bacterial count rather than the somatic cell count...
August 16, 2016: Molecular BioSystems
Philip I Burgess, Simon P Harding, Marta García-Fiñana, Nicholas A V Beare, Gerald Msukwa, Theresa J Allain
PURPOSE: To describe the prevalence, incidence, and progression of retinopathy and to report associations with demographic, clinical, and biochemical variables in people with diabetes in Southern Malawi. DESIGN: Prospective cohort study. PARTICIPANTS: Subjects were systematically sampled from 2 primary care diabetes clinics. METHODS: We performed the first prospective cohort study of diabetic retinopathy from Sub-Saharan Africa over 24 months...
September 2016: Ophthalmology
Chenshu Huang, Shaolin Li
OBJECTIVE: To investigate the relationship between blood neutrophil lymphocyte ratio (NLR) and postmenopausal osteoporosis in Chinese women without diabetes. METHODS: Two hundred and thirty three postmenopausal women without diabetes were included in this study. The clinical data of patients including age, and body mass index (BMI) were recorded. Blood samples were obtained to determine Blood urea nitrogen (BUN), Uric acid (UA), Albumin (ALB), Creatinine (CREA), Total Cholesterol(TC), Triglyceride (TG), Fasting plasma glucose (FPG), Glycosylated Hemoglobin (HbAlc), 25-hydroxyitamin D (25-OHD) level...
May 2016: Pakistan Journal of Medical Sciences Quarterly
Soha M Abd El Dayem, Ahmed A Battah, Amal El Shehaby
AIM: To evaluate cardiac affection in type 1 diabetes in relation to Omentin. PATIENTS AND METHODS: Sixty two diabetics and 30 volunteer of the same age and sex were included as a control group. Blood sample was taken for assessment of omentin and oxidized low density lipoprotein (OxLDL), glycosylated hemoglobin (HbA1) and lipid profile. Urine sample was taken for assessment of albumin/creatinine ratio. 24 hour holter was also done. T-test, simple correlation followed by stepwise multiple regression analysis was used for analysis of data...
December 15, 2015: Open Access Macedonian Journal of Medical Sciences
Soha M Abd El Dayem, Hayam K Nazif, Mona Abd Ei-Kader, Maha El-Tawil
AIM: To study the relation between adiponectin level with glycemic control and complication of diabetes. PATIENTS AND METHODS: The study included 40 female adolescent type 1 diabetic patients and 40 healthy volunteers of the same age and sex. Blood sample was taken for assessment of glycosylated hemoglobin, lipid profile and adiponectine. Urine sample was taken for assessment of albumin/creatinine ratio. RESULTS: Diabetic patients had a significantly higher diastolic blood pressure, triglyceride, total cholesterol, LDL and adiponectin than controls...
December 15, 2015: Open Access Macedonian Journal of Medical Sciences
Nikita M Podvalnyy, Alexander O Chizhov, Alexander I Zinin, Leonid O Kononov
Rapid and simple synthesis of oligosaccharides related to one of the terminal motifs of mycobacterial lipoarabinomannan is described. An array of homologous linear α(1 → 5)-linked oligoarabinofuranosides with 4-(2-chloroethoxy)phenyl aglycon and selectively unprotected 5-OH group at the non-reducing end was obtained by oligomerization of 3-O-benzoyl β-D-arabinofuranose 1,2,5-orthobenzoate. Subsequent introduction of β(1 → 2)-linked arabinofuranose disaccharide moiety by step-wise glycosylation furnished the target oligosaccharides which were conjugated with bovine serum albumin...
August 5, 2016: Carbohydrate Research
Sohaib A Virk, Kim C Donaghue, Yoon Hi Cho, Paul Benitez-Aguirre, Stephen Hing, Alison Pryke, Albert Chan, Maria E Craig
CONTEXT: There is a paucity of data regarding the association between glycosylated hemoglobin (HbA1c) variability and risk of microvascular complications in adolescents with type 1 diabetes (T1D). OBJECTIVE: To investigate the association between HbA1c variability and risk of microvascular complications in adolescents with T1D. DESIGN: Prospective cohort study from 1990 to 2014 (median follow-up, 8.1 y). SETTING: Tertiary pediatric hospital...
September 2016: Journal of Clinical Endocrinology and Metabolism
Xiuling Li, Yuting Xiong, Guangyan Qing, Ge Jiang, Xianqin Li, Taolei Sun, Xinmiao Liang
Abnormal sialylation of proteins is highly associated with many major diseases, such as cancers and neurodegenerative diseases. However, this study is challenging owing to the difficulty in enriching trace sialylated glycopeptides (SGs) from highly complex biosamples. The key to solving this problem relies strongly on the design of novel SG receptors to capture the sialic acid (SA) moieties in a specific and tunable manner. Inspired by the saccharide-saccharide interactions in life systems, here we introduce saccharide-based SG receptors into this study...
June 1, 2016: ACS Applied Materials & Interfaces
Simon Staubach, Murat Pekmez, Franz-Georg Hanisch
Classical galactosemia is caused by a nearly complete deficiency of galactose-1-phosphate uridyltransferase (GALT; EC 2.7.712), resulting in a severely impaired galactose metabolism with galactose-1-phosphate and galactitol accumulation. Even on a galactose-restricted diet, patients develop serious long-term complications of the central nervous system and ovaries that may result from chronic cell-toxic effects exerted by endogenous galactose. To address the question of whether disease-associated cellular perturbations could affect the kidney function of the patients, we performed differential proteomics of detergent-resistant membranes from urinary exovesicles...
June 3, 2016: Journal of Proteome Research
Raphael Reuten, Denise Nikodemus, Maria B Oliveira, Trushar R Patel, Bent Brachvogel, Isabelle Breloy, Jörg Stetefeld, Manuel Koch
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions can cause problems. Here, we investigated expression levels of recombinant extracellular, intracellular as well as transmembrane proteins tethered to different polypeptides in mammalian cell lines. Strikingly, fusion of proteins to the prokaryotic maltose-binding protein (MBP) generally enhanced protein production...
2016: PloS One
Yuki Ichikawa, Satoru Joshita, Takeji Umemura, Yugo Shobugawa, Soichiro Shibata, Tomoo Yamazaki, Naoyuki Fujimori, Michiharu Komatsu, Akihiro Matsumoto, Eiji Tanaka
AIM: Serum glycosylated Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA(+) -M2BP) is a reliable, non-invasive marker of liver fibrosis. This study assessed the ability of WFA(+) -M2BP to diagnose liver fibrosis in patients with chronic hepatitis B virus (HBV) infection and evaluated WFA(+) -M2BP as a predictor of hepatocellular carcinoma (HCC) development. METHODS: Serum WFA(+) -M2BP values were retrospectively evaluated in 112 treatment naïve patients with HBV-related chronic hepatitis and cirrhosis who had undergone liver biopsy at our hospital...
March 29, 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Tomasz Wybranowski, Blanka Ziomkowska, Michał Cyrankiewicz, Stefan Kruszewski
The study can be useful for understanding the interaction of camptothecin with human serum albu- min. There are two forms of camptothecin (the carboxylate form (CPT-C) and the lactone form (CPT-L)) but only the lactone one is pharmacologically active. It was reported earlier that in the presence of HSA, the active lactone form of camptothecin changes to inactive carboxylate form and it reduces the antitumor activities of camptothecin. However, those studies were performed at physiological pH (7.4) and with non-oxidized and non-glycosylated albumin...
January 2016: Acta Poloniae Pharmaceutica
Xiaoyan Xiao, Yajuan Wang, Yanlian Hou, Feng Han, Jianmin Ren, Zhao Hu
OBJECTIVES: To investigate serum 25-hydroxyvitamin D (25[OH]D) levels in patients with diabetic nephropathy, analyse the relationship between 25(OH)D and clinical indexes, and identify risk factors for vitamin D deficiency in diabetic nephropathy. METHODS: Patients with diabetic nephropathy were sequentially enrolled and grouped according to diabetic nephropathy stage. Serum 25(OH)D levels were measured. A control group of healthy subjects was used for comparison...
June 2016: Journal of International Medical Research
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