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https://www.readbyqxmd.com/read/28109026/refinement-of-the-criteria-for-ultrastructural-peritubular-capillary-basement-membrane-multilayering-in-the-diagnosis-of-chronic-active-acute-antibody-mediated-rejection
#1
Heounjeong Go, Sung Shin, Young Hoon Kim, Duck Jong Han, Yong Mee Cho
Chronic active/acute antibody-mediated rejection (cABMR) is the main cause of late renal allograft loss. Severe peritubular capillary basement membrane multilayering (PTCML) assessed on electron microscopy is one diagnostic feature of cABMR according to the Banff2013 classification. We aimed to refine the PTCML criteria for an earlier diagnosis of cABMR. We retrospectively investigated ultrastructural features of 159 consecutive renal allografts and 44 non-allografts. The presence of serum donor-specific antibodies at the time of biopsy of allografts was also examined...
January 21, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28108636/serelaxin-treatment-promotes-adaptive-hypertrophy-but-does-not-prevent-heart-failure-in-experimental-peripartum-cardiomyopathy
#2
Justus Nonhoff, Melanie Ricke-Hoch, Mirco Mueller, Britta Stapel, Tobias Pfeffer, Martina Kasten, Michaela Scherr, Constantin von Kaisenberg, Johann Bauersachs, Arash Haghikia, Denise Hilfiker-Kleiner
AIMS: Peripartum cardiomyopathy (PPCM) is a systolic left ventricular dysfunction developing in the peripartum phase in previously healthy women. Relaxin-2 is a pregnancy hormone with potential beneficial effects in heart failure patients. We evaluated Relaxin-2 as a potential diagnostic marker and/or a therapeutic agent in PPCM. METHODS AND RESULTS: In healthy peripartum women, serum Relaxin-2 levels (measured by ELISA in the second half of pregnancy) were elevated showing a decreasing trend in the first postpartum week and returned to non-pregnant levels thereafter...
January 20, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28108432/living-on-the-edge-conservation-genetics-of-seven-thermophilous-plant-species-in-a-high-arctic-archipelago
#3
Siri Birkeland, Idunn Elisabeth Borgen Skjetne, Anne Krag Brysting, Reidar Elven, Inger Greve Alsos
Small, isolated, and/or peripheral populations are expected to harbour low levels of genetic variation and may therefore have reduced adaptability to environmental change, including climate warming. In the Arctic, global warming has already caused vegetation change across the region and is acting as a significant stressor on Arctic biodiversity. Many of the rare plants in the Arctic are relicts from early Holocene warm periods, but their ability to benefit from the current warming is dependent on the viability of their populations...
January 20, 2017: AoB Plants
https://www.readbyqxmd.com/read/28108081/retention-modeling-and-retention-time-prediction-in-gas-chromatography-and-flow-modulation-comprehensive-two-dimensional-gas-chromatography-the-contribution-of-pressure-on-solute-partition
#4
Antoine Burel, Marie Vaccaro, Yohann Cartigny, Séverine Tisse, Gérard Coquerel, Pascal Cardinael
This study aims at modelling and predicting solute retention in capillary Gas Chromatography (GC) and Flow Modulation comprehensive GC (FM-GCxGC). A new thermodynamic model, taking into account the effects of temperature and pressure, is proposed to describe the variation of the equilibrium partition constant of a solute during its elution. This retention model was challenged with the classical one, and both were applied to: (i) stationary phase film thickness indirect estimation; (ii) retention time (RT) prediction of a set of 11 model polycyclic aromatic hydrocarbons (PAHs) on the SLB-IL60 and DB-35MS columns, in temperature-programmed mode...
January 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28107971/screening-of-enzyme-inhibitors-from-traditional-chinese-medicine-by-magnetic-immobilized-%C3%AE-glucosidase-coupled-with-capillary-electrophoresis
#5
Dong-Mei Liu, Juan Chen, Yan-Ping Shi
As the close correlation between α-glucosidase inhibitors and the treatment of diabetes, in combination with capillary electrophoresis (CE), a method was developed to screen α-glucosidase inhibitors from traditional Chinese medicines (TCMs) by immobilizing α-glucosidase on magnetic nanoparticles. Such a magnetic immobilization would be beneficial for enzyme reusability, stability and separation. In this work, Fe3O4 nanoparticles were synthesized by solvothermal method. And the prepared nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), size and zeta potential analysis...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107940/incorporation-of-zeolitic-imidazolate-framework-zif-8-derived-nanoporous-carbons-in-methacrylate-polymeric-monoliths-for-capillary-electrochromatography
#6
Enrique Javier Carrasco-Correa, Andrea Martínez-Vilata, José Manuel Herrero-Martínez, José B Parra, Fernando Maya, Victor Cerdà, Carlos Palomino Cabello, Gemma Turnes Palomino, Frantisek Svec
A series of metal organic frameworks-derived nanoporous carbons originating from zeolitic imidazolate framework-8 (ZIF-8) crystals as precursors have been prepared via varying the preparation conditions. The ZIF-8-derived carbons were subsequently admixed in the methacrylate monomers containing polymerization mixtures and polymerized to obtain monolithic columns for capillary electrochromatography (CEC). The effect of particle size and content of the ZIF-8-derived carbon materials in the polymerization mixture on the performance of the hybrid monolithic columns was investigated in detail...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107926/detection-of-silver-nanoparticles-in-seawater-at-ppb-levels-using-uv-visible-spectrophotometry-with-long-path-cells
#7
Pablo Lodeiro, Eric P Achterberg, Mohammad S El-Shahawi
Silver nanoparticles (AgNPs) are emerging contaminants that are difficult to detect in natural waters. UV-visible spectrophotometry is a simple technique that allows detection of AgNPs through analysis of their characteristic surface plasmon resonance band. The detection limit for nanoparticles using up to 10cm path length cuvettes with UV-visible spectrophotometry is in the 0.1-10ppm range. This detection limit is insufficiently low to observe AgNPs in natural environments. Here we show how the use of capillary cells with an optical path length up to 200cm, forms an excellent technique for rapid detection and quantification of non-aggregated AgNPs at ppb concentrations in complex natural matrices such as seawater...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107920/evaluation-of-liquid-cathode-glow-discharge-atomic-emission-spectrometry-for-determination-of-copper-and-lead-in-ores-samples
#8
Jie Yu, Shuxiu Yang, Quanfang Lu, Duixiong Sun, Jidong Zheng, Xiaomin Zhang, Xing Wang, Wu Yang
In this study, a liquid cathode glow discharge-atomic emission spectrometry (LCGD-AES) was constructed for simultaneously determination of Cu and Pb in digested ores samples, in which the glow discharge was produced between the needle-like Pt anode and electrolyte overflowing from quartz capillary. The stability of LCGD and the effects of discharge voltage, capillary diameter and flow rate on emission intensity were systematically investigated. The limits of detections (LODs) of Cu and Pb were compared with those measured by closed-type electrolyte cathode discharge-atomic emission spectrometry (ELCAD-AES)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107909/trapping-magnetic-nanoparticles-for-in-line-capillary-electrophoresis-in-a-liquid-based-capillary-coolant-system
#9
Pranov Ramana, Erwin Adams, Patrick Augustijns, Ann Van Schepdael
A method to trap magnetic nanoparticles inside the electrophoretic capillary has been developed without disabling and disturbing the coolant liquid recirculation. The complete optimization from setting up of the external magnetic field to injecting the magnetic nanoparticles in order to facilitate efficient trapping is described. In addition to this, the setup has been tested with 150mM sodium phosphate buffer as the background electrolyte at different pH values (2, 4, 6 and 8). All these pH values have been investigated at 25°C and 37°C to mimic room temperature and physiological temperature, respectively...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107758/retroperitoneal-unicentric-castleman-s-disease-a-case-report
#10
Nihed Abdessayed, Ahlem Bdioui, Houssem Ammar, Rahul Gupta, Nozha Mhamdi, Marwa Guerfela, Moncef Mokni
INTRODUCTION: Castleman's disease (CD) is an angio-follicular lymph node hyperplasia presenting as a localized or a systemic disease masquerading malignancy. The most common sites of CD are mediastinum, neck, axilla and pelvis. Unicentric CD in the peripancreatic region is very rare. PRESENTATION OF CASE: We report a case of the 34-year-old lady presenting with epigastric pain for 3 months. Abdominal imaging revealed a retroperitoneal mass arising from the pancreas suspected to be neuroendocrine tumor...
January 5, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28107170/hb-variants-in-korea-effect-on-hba1c-using-five-routine-methods
#11
Yeo-Min Yun, Misuk Ji, Dae-Hyun Ko, Sail Chun, Gye Cheol Kwon, Kyunghoon Lee, Sang Hoon Song, Moon Woo Seong, Sung Sup Park, Junghan Song
BACKGROUND: Quantification of glycated hemoglobin (HbA1c) is a challenge in patients with hemoglobin (Hb) variants. We evaluated the impact of various Hb variants on five routine HbA1c assays by comparing with the IFCC reference measurement procedure (RMP). METHODS: Whole blood samples showing warning flags or no results on routine HPLC HbA1c assays were confirmed for Hb variants and were submitted to HbA1c quantification using Sebia Capillarys 2 Flex Piercing, Roche Tina-quant HbA1c Gen...
January 20, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28106938/vascular-endothelial-growth-factor-targeted-paclitaxel-loaded-liposome-microbubbles-and-inhibition-of-human-epidermoid-2-cell-proliferation
#12
Lian Zhou, Wenlong Luo
BACKGROUND: Vascular endothelial growth factor (VEGF) plays an important role in the formation of capillary blood vessels. The purpose of this study was to evaluate the inhibitory effect of VEGF-targeted paclitaxel (PTX)-loaded liposome microbubbles (VTPLLMs) on the proliferation of human epidermoid (Hep-2) laryngeal squamous cell carcinoma (SCC). METHODS: Six groups were randomly divided. The inhibitory effects on Hep-2 proliferation were assessed by a methylthiazol tetrazolium (MTT) method...
January 20, 2017: Head & Neck
https://www.readbyqxmd.com/read/28106702/capillary-hemangioma-of-the-tympanic-membrane-and-external-auditory-canal
#13
Seo Bin Kim, Hwan-Ho Lee
Hemangiomas are very common tumors characterized by large numbers of vessels filled with blood. Capillary hemangioma is the most common type of hemangioma, whereas capillary hemangiomas of the tympanic membrane and external auditory canal are extremely rare vascular tumors. Here, the authors present capillary hemangioma of the tympanic membrane and external auditory canal in a 54-year-old woman with right-sided aural fullness for 3 months. Under local anesthesia, the mass was resected by transcanal microscopic surgery...
January 18, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28106629/heat-shock-protein-70-overexpression-in-adipose-derived-stem-cells-enhances-fat-graft-survival
#14
Hao Feng, Lihong Qiu, Teng Zhang, Houyou Yu, Xianjie Ma, Yingjun Su, Hui Zheng, Yong Wang, Chenggang Yi
BACKGROUND: Autologous fat grafting is a prevalent technique used for soft-tissue augmentation; however, the poor survival rate of the grafted tissue remains a drawback of this method. Although adipose-derived stem cells (ASCs) are an attractive candidate for enhancing graft retention, the poor posttransplantation viability of these cells limits their application. Here we investigated whether overexpression of the antiapoptotic protein heat-shock protein 70 (Hsp70) could enhance ASCs' therapeutic potential for fat transplant survival...
January 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28106334/rapid-determination-of-the-critical-micelle-concentration-by-taylor-dispersion-analysis-in-capillaries-using-both-direct-and-indirect-detection
#15
Jan Petr
The critical micelle concentration belongs is a basic characteristic of surfactants. In this paper, the process of formation of micelles is studied by Taylor dispersion analysis using capillary electrophoresis instrumentation as a substituent of capillary liquid chromatography. New methods for determination of critical micelle concentration are presented. Sodium octylbenzene sulfonate and sodium dodecylsulfate are used as model compounds with and without chromophore. Two novel approaches based on indirect UV detection and indirect Taylor dispersion analysis with direct UV detection are introduced for the determination of critical micelle concentration value of surfactant without any chromophore...
January 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28106307/normal-saline-is-associated-with-increased-sickle-red-cell-stiffness-and-prolonged-transit-times-in-a-microfluidic-model-of-the-capillary-system
#16
Marcus A Carden, Meredith Fay, Yumiko Sakurai, Brynn McFarland, Sydney Blanche, Caleb Diprete, Clinton H Joiner, Todd Sulchek, Wilbur A Lam
Vaso-occlusive crisis (VOC) is a complex process that occurs in patients with sickle cell disease (SCD) and is often associated with pain and urgent hospitalization. A major instigator of VOC is microvascular obstruction by pathologically stiffened sickle red blood cells (RBCs), and thus therapy relies heavily on optimizing intravenous fluid (IVF) hydration to increase RBC deformability. However, no evidence-based guidelines regarding the choice of IVF currently exist. We therefore analyzed alterations in biomechanical properties of sickle RBCs isolated from patients with homozygous SCD (hemoglobin SS) after exposure to different osmolarities of clinical IVF formulations...
January 20, 2017: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/28106268/innate-immune-defense-in-the-inner-ear-mucines-are-expressed-by-the-human-endolymphatic-sac
#17
Martin N Møller, Svend Kirkeby, Per Cayé-Thomasen
The human endolymphatic sac has been shown recently to have immunological capacities and has thus been proposed as the main entity protecting the inner ear from pathogen invasion, equivalent to mucosa-associated lymphoid tissue (MALT). Although the sac expresses molecules of the innate immune system, the potential expression of members of the important mucin family has not been detailed. Thus, this paper explores endolymphatic sac expression of a number of mucins and mucin precursors. Twelve fresh tissue samples from the human endolymphatic sac were obtained during translabyrinthine surgery...
February 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28106251/affinity-capillary-electrophoresis-and-quantum-mechanical-calculations-applied-to-investigation-of-gly-6-antamanide-binding-with-sodium-and-potassium-ions
#18
Sachin Pangavhane, Stanislav Böhm, Emanuel Makrlík, Paolo Ruzza, Václav Kašička
Affinity capillary electrophoresis in a free solution (ACE) and quantum mechanical density functional theory (DFT) have been applied to the investigation of interactions of glycine-6-antamanide ([Gly(6) ]AA), a synthetic derivative of cyclic decapeptide antamanide isolated from the highly poisonous mushroom Amanita phalloides, with sodium or potassium ions in methanol. First, from the dependence of effective electrophoretic mobility of [Gly(6) ]AA on Na(+) or K(+) ions concentration in the background electrolyte (BGE) (methanolic solution of 20 mM chloroacetic acid, 10 mM Tris, pHMeOH 7...
January 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28105972/comparative-validation-of-the-determination-of-sofosbuvir-in-pharmaceuticals-by-several-inexpensive-ecofriendly-chromatographic-electrophoretic-and-spectrophotometric-methods
#19
Amira F El-Yazbi
Sofosbuvir (SOFO) was approved by the U.S. Food and Drug Administration in 2013 for the treatment of hepatitis C virusinfection with enhanced antiviral potency compared with earlier analogs. Notwithstanding, all current editions of the pharmacopeias still do not present any analytical methods for the quantification of SOFO. Thus, rapid, simple, and ecofriendly methods for the routine analysis of commercial formulations of SOFO are desirable. In this study, five accurate methods for the determination of SOFO in pharmaceutical tablets were developed and validated...
January 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28105847/compact-ultrahigh-pressure-nanoflow-capillary-liquid-chromatograph
#20
Xiaofeng Zhao, Xiaofeng Xie, Sonika Sharma, Luke T Tolley, Alex Plistil, Hal E Barnett, Martin P Brisbin, Adam C Swensen, John C Price, Paul B Farnsworth, H Dennis Tolley, Stanley D Stearns, Milton L Lee
A compact ultrahigh-pressure nanoflow liquid chromatograph (LC) was developed with the purpose in mind of creating a portable system that could be easily moved to various testing locations or placed in close proximity to other instruments for optimal coupling, such as with mass spectrometry (MS). The system utilized innovative nanoflow pumps integrated with a very low volume stop-flow injector and mixing tee. The system weighed only 5.9 kg (13 lbs) or 4.5 kg (10 lbs) without a controller and could hold up to 1100 bar (16000 psi) of pressure...
January 3, 2017: Analytical Chemistry
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