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https://www.readbyqxmd.com/read/28938153/massively-parallel-sequencing-of-32-forensic-markers-using-the-precision-id-globalfiler%C3%A2-ngs-str-panel-and-the-ion-pgm%C3%A2-system
#1
Zheng Wang, Di Zhou, Hui Wang, Zhenjun Jia, Jing Liu, Xiaoqin Qian, Chengtao Li, Yiping Hou
Massively parallel sequencing (MPS) technologies have proved capable of sequencing the majority of the key forensic STR markers. By MPS, not only the repeat-length size but also sequence variations could be detected. Recently, Thermo Fisher Scientific has designed an advanced MPS 32-plex panel, named the Precision ID GlobalFiler™ NGS STR Panel, where the primer set has been designed specifically for the purpose of MPS technologies and the data analysis are supported by a new version HID STR Genotyper Plugin (V4...
September 8, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28938000/podoplanin-increases-the-migration-of-human-fibroblasts-and-affects-the-endothelial-cell-network-formation-a-possible-role-for-cancer-associated-fibroblasts-in-breast-cancer-progression
#2
Jaroslaw Suchanski, Anna Tejchman, Maciej Zacharski, Aleksandra Piotrowska, Jedrzej Grzegrzolka, Grzegorz Chodaczek, Katarzyna Nowinska, Janusz Rys, Piotr Dziegiel, Claudine Kieda, Maciej Ugorski
In our previous studies we showed that in breast cancer podoplanin-positive cancer-associated fibroblasts correlated positively with tumor size, grade of malignancy, lymph node metastasis, lymphovascular invasion and poor patients' outcome. Therefore, the present study was undertaken to assess if podoplanin expressed by fibroblasts can affect malignancy-associated properties of breast cancer cells. Human fibroblastic cell lines (MSU1.1 and Hs 578Bst) overexpressing podoplanin and control fibroblasts were co-cultured with breast cancer MDA-MB-231 and MCF7 cells and the impact of podoplanin expressed by fibroblasts on migration and invasiveness of breast cancer cells were studied in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28937866/soluble-vascular-adhesion-protein-1-mediates-spermine-oxidation-as-semicarbazide-sensitive-amine-oxidase-possible-role-in-proliferative-diabetic-retinopathy
#3
Miyuki Murata, Kousuke Noda, Akiko Kawasaki, Shiho Yoshida, Yoko Dong, Michiyuki Saito, Zhenyu Dong, Ryo Ando, Shohei Mori, Wataru Saito, Atsuhiro Kanda, Susumu Ishida
Purpose/Aim of the study: To explore the possible role of vascular adhesion protein-1 (VAP-1) via its enzymatic function as a semicarbazide-sensitive amine oxidase (SSAO) in the pathogenesis of proliferative diabetic retinopathy (PDR). MATERIALS AND METHODS: The levels of soluble VAP-1/SSAO and the unsaturated aldehyde acrolein (ACR)-conjugated protein, N(ε)-(3-formyl-3, 4-dehydropiperidino) lysine adduct (FDP-Lys), were measured in vitreous fluid samples of PDR and non-diabetic patients using ELISA...
September 22, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28937238/effects-of-roflumilast-a-phosphodiesterase-4-inhibitor-on-the-lung-functions-in-a-saline-lavage-induced-model-of-acute-lung-injury
#4
P Kosutova, P Mikolka, M Kolomaznik, S Rezakova, A Calkovska, D Mokra
Acute lung injury (ALI) is associated with deterioration of alveolar-capillary lining and transmigration and activation of inflammatory cells. Whereas a selective phosphodiesterase-4 (PDE4) inhibitor roflumilast has exerted potent anti-inflammatory properties, this study evaluated if its intravenous delivery can influence inflammation, edema formation, and respiratory parameters in rabbits with a lavage-induced model of ALI. ALI was induced by repetitive saline lung lavage (30 ml/kg). Animals were divided into 3 groups: ALI without therapy (ALI), ALI treated with roflumilast i...
September 22, 2017: Physiological Research
https://www.readbyqxmd.com/read/28936439/a-case-of-pulmonary-arteriovenous-malformation-role-of-interventional-radiology-in-diagnosis-and-treatment
#5
Pranav Sharma, Puneet Kochar, Salil Sharma, Nishant Gupta, Shuo Li, Kusum Hooda, Yogesh Kumar
Pulmonary arterio-venous malformations (PAVMs) are abnormal pulmonary arteries and pulmonary veins communicating directly without interposition of a capillary bed and about 80-90% of patients with PAVMs eventually may present with hereditary hemorrhagic telangiectasia (HHT), remaining ones are sporadic cases. On the other hand, about 15-35% of HHT patients may present with PAVMs. The PAVMs have a tendency to grow and increase in size over time and various factors like puberty, pregnancy and pulmonary arterial hypertension (PAH) affect growth...
September 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28936269/microfibrous-scaffolds-enhance-endothelial-differentiation-and-organization-of-induced-pluripotent-stem-cells
#6
Joseph J Kim, Luqia Hou, Guang Yang, Nicholas P Mezak, Maureen Wanjare, Lydia M Joubert, Ngan F Huang
INTRODUCTION: Human induced pluripotent stem cells (iPSCs) are a promising source of endothelial cells (iPSC-ECs) for engineering three-dimensional (3D) vascularized cardiac tissues. To mimic cardiac microvasculature, in which capillaries are oriented in parallel, we hypothesized that endothelial differentiation of iPSCs within topographically aligned 3D scaffolds would be a facile one-step approach to generate iPSC-ECs as well as induce aligned vascular organization. METHODS: Human iPSCs underwent endothelial differentiation within electrospun 3D polycaprolactone (PCL) scaffolds having either randomly oriented or parallel-aligned microfibers...
October 2017: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/28935974/membrane-particles-generated-from-mesenchymal-stromal-cells-modulate-immune-responses-by-selective-targeting-of-pro-inflammatory-monocytes
#7
Fabiany da C Gonçalves, Franka Luk, Sander S Korevaar, Rachid Bouzid, Ana H Paz, Carmen López-Iglesias, Carla C Baan, Ana Merino, Martin J Hoogduijn
Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935657/use-of-hybrid-capillary-tube-setup-on-400mhz-nmr-for-quantitation-of-crucial-low-quantity-metabolites-using-asicco-signal
#8
Ranjeet Tiwari, Deepak Ahire, Hemantha Kumar, Sarmistha Sinha, Siddheshwar Kisan Chauthe, Murali Subramanian, Ramaswamy Iyer, Ramakanth Sarabu, Lakshmi Kant Bajpai
Metabolites of new chemical entities can influence safety and efficacy of a molecule and often times need to be quantified in preclinical studies. However, synthetic standards of metabolites are very rarely available in early discovery. Alternate approaches such as bio-synthesis needs to be explored to generate these metabolites. Assessing the quantity and purity of these small amounts of metabolites with a non-destructive analytical procedure becomes crucial. Quantitative nuclear magnetic resonance (qNMR) becomes the method of choice for these samples...
September 21, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28935506/endothelial-specific-sirt3-deletion-impairs-glycolysis-and-angiogenesis-and-causes-diastolic-dysfunction
#9
Xiaochen He, Heng Zeng, Sean T Chen, Richard J Roman, Judy L Aschner, Sean Didion, Jian-Xiong Chen
Endothelial glycolysis plays a critical role in the regulation of angiogenesis. We investigated the role of Sirtuin 3 (SIRT3) on endothelial cell (EC) glycolytic metabolism, angiogenesis, and diastolic function. Our aim was to test the hypothesis that loss of SIRT3 in ECs impairs endothelial glycolytic metabolism and angiogenesis and contributes to myocardial capillary rarefaction and the development of diastolic dysfunction. Using SIRT3 deficient ECs, SIRT3 was found to regulate a metabolic switch between mitochondrial respiration and glycolysis...
September 18, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28934255/quantitative-optical-coherence-tomography-angiography-of-macular-vascular-structure-and-foveal-avascular-zone-in-glaucoma
#10
Jaewan Choi, Junki Kwon, Joong Won Shin, Jiyun Lee, Saem Lee, Michael S Kook
OBJECTIVE: The study aimed to evaluate the quantitative characteristics of the macular vessel density (VD) and foveal avascular zone (FAZ) in glaucoma using optical coherence tomography angiography (OCT-A). DESIGN: Cross-sectional, age- and sex-matched case-control study. METHODS: Fifty-two eyes of 52 patients with primary open angle glaucoma and 52 eyes from 52 healthy participants were recruited retrospectively. OCT-A was performed on a 3 x 3-mm macular region centered on the fovea...
2017: PloS One
https://www.readbyqxmd.com/read/28933862/creim-coffee-ring-effect-imaging-model-for-monitoring-protein-self-assembly-in-situ
#11
Michael Shaw, Angelo Bella, Maxim G Ryadnov
Protein self-assembly is fundamental to nanotechnology. Self-assembling structures are produced under static in vitro conditions typically forming over hours. In contrast, hydrodynamic intracellular environments employ far shorter time scales to compartmentalize highly concentrated protein solutions. Herein, we exploit the radial capillary flow within a drying sessile droplet (the coffee ring effect) to emulate dynamic native environments and monitor an archetypal protein assembly in situ using high-speed super-resolution imaging...
September 22, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28933795/microfluidic-production-of-nanoscale-perfluorocarbon-droplets-as-liquid-contrast-agents-for-ultrasound-imaging
#12
Xiaonan Xu, Ruyuan Song, Minghao He, Chuan Peng, Miao Yu, Youmin Hou, Huihe Qiu, Ruhai Zou, Shuhuai Yao
Liquid perfluorocarbon (PFC) nanodroplets may have a better chance to extravasate through inter-endothelial gaps (400-800 nm) into tumor interstitium for extravascular imaging, which holds promise as an innovative strategy for imaging-guided drug delivery, early diagnosis of cancer and minimally invasive treatment of cancer. Currently available emulsion technologies still face challenges in reducing droplet sizes from the microscale to the nanoscale. To control size and ensure monodispersity of PFC nanodroplets, we developed a flame-shaped glass capillary and polydimethylsiloxane (PDMS) hybrid device that creates a concentric flow of the dispersed phase enclosed by the focusing continuous phase at the cross-junction...
September 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28933341/c4d-expressing-glomerulopathy-and-proteinuria-post-transplantation-of-a-%C3%A2-too-big-for-size-mismatched-kidney-allograft-an-unusual-case-with-good-outcome%C3%A2
#13
Francois Gougeon, Alexei V Mikhailov, Keisha Gibson, Tomasz Kozlowski, Harsharan K Singh, Volker Nickeleit
A 5-year-old severely growth-retarded child with tubulointerstitial, oliguric end-stage renal disease received an adult-size kidney transplant. Three years post grafting under standard triple immunosuppression (mycophenolate mofetil, tacrolimus, and prednisone) de novo nephrotic range proteinuria without the nephrotic syndrome developed. Graft function was normal (serum creatinine: 0.2 - 0.3 mg/dL), there were no donor-specific HLA antibodies (DSA), and the urine sediment was inactive. Two biopsies collected 3 and 4 years post-transplantation showed severe glomerular capillary wall remodeling and associated pseudolinear C4d staining as morphologic correlates for the proteinuria...
September 21, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28933255/organizational-hierarchy-and-structural-diversity-of-microvascular-pericytes-in-adult-mouse-cortex
#14
Roger I Grant, David A Hartmann, Robert G Underly, Andrée-Anne Berthiaume, Narayan R Bhat, Andy Y Shih
Smooth muscle cells and pericytes, together called mural cells, coordinate many distinct vascular functions. Canonically, smooth muscle cells are ring-shaped and cover arterioles with circumferential processes, whereas pericytes extend thin processes that run longitudinally along capillaries. In between these canonical mural cell types are cells with features of both smooth muscle cells and pericytes. Recent studies suggest that these transitional cells are critical for controlling blood flow to the capillary bed during health and disease, but there remains confusion on how to identify them and where they are located in the brain microvasculature...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/28932942/required-hydrophobicity-of-fluorescent-reporters-for-phosphatidylinositol-family-of-lipid-enzymes
#15
Jarod Waybright, Weigang Huang, Angela Proctor, Xiaoyang Wang, Nancy L Allbritton, Qisheng Zhang
The phosphatidylinositol (PtdIns) family of lipids plays important roles in cell differentiation, proliferation, and migration. Abnormal expression, mutation, or regulation of their metabolic enzymes has been associated with various human diseases such as cancer, diabetes, and bipolar disorder. Recently, fluorescent derivatives have increasingly been used as chemical probes to monitor either lipid localization or enzymatic activity. However, the requirements of a good probe have not been well defined, particularly modifications on the diacylglycerol side chain partly due to challenges in generating PtdIns lipids...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28932887/microchip-capillary-gel-electrophoresis-combined-with-lectin-affinity-enrichment-employing-magnetic-beads-for-glycoprotein-analysis
#16
Nicole Y Engel, Victor U Weiss, Christian Wenz, Susanne Glück, Andreas Rüfer, Martin Kratzmeier, Martina Marchetti-Deschmann, Günter Allmaier
Due to the constant search for reliable methods to investigate glycoproteins in complex biological samples, an alternative approach combining affinity enrichment with rapid and sensitive analysis on-a-chip is presented. Glycoproteins were specifically captured by lectin-coated magnetic beads, eluted by competitive sugars, and investigated with microchip capillary gel electrophoresis (MCGE), i.e., CGE-on-a-chip. We compared our results to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) data, which turned out to be in very good agreement...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28932732/sustained-released-mixture-of-vascular-endothelial-growth-factor-165-and-fibrin-glue-strengthens-healing-of-ileal-anastomoses-in-a-rabbit-model-with-intraperitoneal-infection
#17
Zhanwu Li, Wenjun Wang, Xiaozhou Wang, Lei Jiang, Fengyi Wang, Qiang Liu
PURPOSE: To investigate the effects of a sustained-released mixture of vascular endothelial growth factor 165 (VEGF165) and fibrin glue (FG) local administration on postoperative rabbit ileal anastomoses. METHODS: One hundred twenty-eight male and female New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 groups, including 32 animals in each, applied with saline solution, FG, rhVEGF165 and a mixture of rhVEGF165 with FG (VEGF + FG) on the anastomoses, respectively...
September 2017: Annals of Surgical Treatment and Research
https://www.readbyqxmd.com/read/28932571/management-of-pulmonary-hypertension-from-left-heart-disease-in-candidates-for-orthotopic-heart-transplantation
#18
REVIEW
Anna Koulova, Alan L Gass, Saikrishna Patibandla, Chhaya Aggarwal Gupta, Wilbert S Aronow, Gregg M Lanier
Pulmonary hypertension in left heart disease (PH-LHD) commonly complicates prolonged heart failure (HF). When advanced, the PH becomes fixed or out of proportion and is associated with increased morbidity and mortality in patients undergoing orthotopic heart transplant (OHT). To date, the only recommended treatment of out of proportion PH is the treatment of the underlying HF by reducing the pulmonary capillary wedge pressure (PCWP) with medications and often along with use of mechanical circulatory support...
August 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28931872/efficient-steam-generation-by-inexpensive-narrow-gap-evaporation-device-for-solar-applications
#19
Matteo Morciano, Matteo Fasano, Uktam Salomov, Luigi Ventola, Eliodoro Chiavazzo, Pietro Asinari
Technologies for solar steam generation with high performance can help solving critical societal issues such as water desalination or sterilization, especially in developing countries. Very recently, we have witnessed a rapidly growing interest in the scientific community proposing sunlight absorbers for direct conversion of liquid water into steam. While those solutions can possibly be of interest from the perspective of the involved novel materials, in this study we intend to demonstrate that efficient steam generation by solar source is mainly due to a combination of efficient solar absorption, capillary water feeding and narrow gap evaporation process, which can also be achieved through common materials...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931839/multiwell-capillarity-based-microfluidic-device-for-the-study-of-3d-tumour-tissue-2d-endothelium-interactions-and-drug-screening-in-co-culture-models
#20
María Virumbrales-Muñoz, José María Ayuso, Marta Olave, Rosa Monge, Diego de Miguel, Luis Martínez-Lostao, Séverine Le Gac, Manuel Doblare, Ignacio Ochoa, Luis J Fernandez
The tumour microenvironment is very complex, and essential in tumour development and drug resistance. The endothelium is critical in the tumour microenvironment: it provides nutrients and oxygen to the tumour and is essential for systemic drug delivery. Therefore, we report a simple, user-friendly microfluidic device for co-culture of a 3D breast tumour model and a 2D endothelium model for cross-talk and drug delivery studies. First, we demonstrated the endothelium was functional, whereas the tumour model exhibited in vivo features, e...
September 20, 2017: Scientific Reports
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