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https://www.readbyqxmd.com/read/29335865/beneficial-effect-of-berberis-amurensis-rupr-on-penile-erection
#1
Rui Tan, Yun Jung Lee, Kyung Woo Cho, Dae Gill Kang, Ho Sub Lee
OBJECTIVE: To investigate whether the methanol extract of Berberis amurensis Rupr. (BAR) augments penile erection using in vitro and in vivo experiments. METHODS: The ex vivo study used corpus cavernosum strips prepared from adult male New Zealand White rabbits. In in vivo studies for intracavernous pressure (ICP), blood pressure, mean arterial pressure (MAP), and increase of peak ICP were continuously monitored during electrical stimulation of Sprague-Dawley rats...
January 15, 2018: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29333618/age-associated-urethral-dysfunction-in-urethane-anesthetized-rats
#2
Ryu Kimura, Minoru Miyazato, Asuka Ashikari, Takuma Oshiro, Seiichi Saito
AIMS: We aimed to investigate the age-associated changes in movement coordination between the urinary bladder and the urethra in rats. METHODS: A total of 17 female Sprague-Dawley rats were used. The rats were divided into young (3 months old) and middle-aged (12-15 months old) groups. In both groups, isovolumetric cystometry and urethral perfusion pressure (UPP) measurements were performed under urethane anesthesia. After the rhythmic bladder contractions stabilized, L-arginine, a nitric oxide (NO) substrate (100 mg/kg), was administered intravenously in both groups...
January 15, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29333036/autonomic-receptors-and-nitric-oxide-involvements-in-mediating-vasorelaxation-effect-induced-by-syzygium-polyanthum-leaves-extract
#3
Azlini Ismail, Wan Amir Nizam Wan Ahmad
Context: Syzygium polyanthum (Wight) Walp leaves are traditionally used by Malays for treating hypertension. Our previous study showed that aqueous extract of S. polyanthum (AESP) and methanolic extract of S. polyanthum (MESP) extracts of S. polyanthum leaves significantly reduced blood pressure of normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Aims: This study aimed to investigate their vasorelaxation potential and the possible involvement of autonomic receptors and nitric oxide in mediating their effect...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29330450/c-terminal-short-arginine-serine-repeat-sequence-dependent-regulation-of-y14-rbm8a-localization
#4
Takanori Tatsuno, Yasuhito Ishigaki
Y14 (RBM8A) is an RNA recognition motif-containing protein that forms heterodimers with MAGOH and serves as a core factor of the RNA surveillance machinery for the exon junction complex (EJC). The role of the Y14 C-terminal serine/arginine (RS) repeat-containing region, which has been reported to undergo modifications such as phosphorylation and methylation, has not been sufficiently investigated. Thus, we aimed to explore the functional significance of the Y14 C-terminal region. Deletion or dephosphorylation mimic mutants of the C-terminal region showed a shift in localization from the nucleoplasmic region; in addition, the C-terminal RS repeat-containing sequence itself exhibited the potential for nucleolar localization...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329462/silibinin-from-silybum-marianum-stimulates-embryonic-stem-cell-vascular-differentiation-via-the-stat3-pi3-k-akt-axis-and-nitric-oxide
#5
Enas Hussein Ali, Fatemeh Sharifpanah, Maria Wartenberg, Heinrich Sauer
Silibinin, the bioactive compound of milk thistle (Silybum marianum), exerts tissue protective and regenerative effects that may include stem cell differentiation toward vascular cells. The purpose of the present study was to investigate whether silibinin stimulates blood vessel formation from mouse embryonic stem (ES) cells and to unravel the underlying signaling cascade. Vascular branching points were assessed by confocal laser scanning microscopy and computer-assisted image analysis of CD31-positive cell structures...
January 12, 2018: Planta Medica
https://www.readbyqxmd.com/read/29315228/contribution-of-secondary-metabolites-to-the-gastroprotective-effect-of-aqueous-extract-of-ximenia-americana-l-olacaceae-stem-bark-in-rats
#6
Ticiana Parente Aragão, Lady Dayane Kalline Travassos Dos Prazeres, Samara Alves Brito, Pedro José Rolim Neto, Larissa Araújo Rolim, Jackson Roberto Guedes da Silva Almeida, Germana Freire Rocha Caldas, Almir Gonçalves Wanderley
Ximenia americana L. (Olacaceae) is used in ethnomedicine as cicatrizant and for the treatment of gastric disorders. This study identified the chemical constituents of the aqueous extract of X. americana (XaAE) and evaluated its antiulcerogenic activity. After lyophilization, XaAE was analyzed by liquid chromatography-mass spectrometry (LC-MS) and its antiulcerogenic effect was evaluated in acute gastric lesions induced by ethanol, acidified ethanol, and indomethacin. Antisecretory action, mucus production and the participation of sulfhydryl groups (-SH) and nitric oxide (NO) were also investigated...
January 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29313295/radiation-preparation-of-l-arginine-acrylic-acid-hydrogel-matrix-patch-for-transdermal-delivery-of-propranolol-hcl-in-hypertensive-rats
#7
Rasha R Radwan, Heba A Mohamed, H E Ali, Ghada A Mahmoud
In the present study, L-arginine/acrylic acid (Arg/AAc) batch hydrogel was successfully prepared by gamma irradiation for transdermal delivery of propranolol HCl in hypertensive rats. The resulted system has been characterized by FTIR to confirm the hydrogel formation. The swelling behavior of the prepared hydrogels was investigated as a function of time and pH. The kinetics of swelling has been investigated. In vivo pharmacokinetics evaluation, skin irritation test, and histopathological studies were investigated...
January 8, 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/29301528/asymmetric-dimethylarginine-adma-symmetric-dimethylarginine-sdma-and-homoarginine-harg-the-adma-sdma-and-harg-paradoxes
#8
EDITORIAL
Dimitrios Tsikas, Alexander Bollenbach, Erik Hanff, Arslan Arinc Kayacelebi
NG-Methylation of L-arginine (Arg) residues in certain proteins by protein arginine methyltransferases and subsequent proteolysis yields NG-monomethyl-L-arginine (MMA), NG,NG-dimethyl-L-arginine (asymmetric dimethylarginine, ADMA) and NG,N'G-dimethyl-L-arginine (symmetric dimethylarginine, SDMA). Biological MMA, ADMA and SDMA occur as free acids in the nM-range and as residues of proteins of largely unknown quantity. Arginine:glycine amidinotransferase (AGAT) catalyzes the synthesis of L-homoarginine (hArg) from free Arg and L-lysine...
January 4, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29290188/the-potential-therapeutic-role-of-peroxisome-proliferatoractivated-receptors-agonist-in-preeclamptic-pregnant-rats
#9
Hayam Ibrahim Gad Allam, Abeer AbdulMoati Al Masri
OBJECTIVE: To investigate the role of peroxisome proliferator-activated receptor (PPAR)-γ agonist on renal functions and blood levels of vasoregulatory peptides by administering rosiglitazone-a PPAR-γ agonist- to nitric oxide (NO) synthase lacking pregnant rats as a creature model of preeclampsia. STUDY DESIGN: Experimental study. PLACE AND DURATION OF STUDY: Medical College at King Khaled University Hospital, Riyadh, Saudi Arabia, from July 2016 to May 2017...
January 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29281014/systematic-genetic-interaction-studies-identify-histone-demethylase-utx-as-potential-target-for-ameliorating-huntington-s-disease
#10
Wan Song, Nóra Zsindely, Anikó Faragó, J Lawrence Marsh, László Bodai
Huntington's Disease (HD) is a dominantly inherited neurodegenerative disease caused by alterations in the huntingtin gene (htt). Transcriptional dysregulation is an early event in HD progression. Protein acetylation and methylation particularly on histones regulates chromatin structure thereby preventing or facilitating transcription. Although protein acetylation has been found to affect HD symptoms, little is known about the potential role of protein methylation in HD pathology. In recent years, a series of proteins have been described that are responsible for methylating and demethylating histones as well as other proteins...
December 21, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29275330/structural-basis-for-substrate-specificity-of-methylsuccinyl-coa-dehydrogenase-an-unusual-member-of-the-acyl-coa-dehydrogenase-family
#11
Thomas Schwander, Richard McLean, Jan Zarzycki, Tobias J Erb
(2S)-methylsuccinyl-CoA dehydrogenase (MCD) belongs to the family of FAD-dependent acyl-CoA dehydrogenase (ACD) and is a key enzyme of the ethylmalonyl-CoA pathway for acetate assimilation. It catalyzes the oxidation of (2S)-methylsuccinyl-CoA to α,β-unsaturated mesaconyl-CoA and shows only about 0.5% activity with succinyl-CoA. Here we report the crystal structure of MCD at a resolution of 1.37 Å. The enzyme forms a homodimer of two 60 kDa subunits. Compared to other ACDs, MCD contains a ~170 residue long N-terminal extension that structurally mimics a dimer-dimer interface of these enzymes that are canonically organized as tetramers...
December 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29262329/prmt5-is-a-critical-regulator-of-breast-cancer-stem-cell-function-via-histone-methylation-and-foxp1-expression
#12
Kelly Chiang, Agnieszka E Zielinska, Abeer M Shaaban, Maria Pilar Sanchez-Bailon, James Jarrold, Thomas L Clarke, Jingxian Zhang, Adele Francis, Louise J Jones, Sally Smith, Olena Barbash, Ernesto Guccione, Gillian Farnie, Matthew J Smalley, Clare C Davies
Breast cancer progression, treatment resistance, and relapse are thought to originate from a small population of tumor cells, breast cancer stem cells (BCSCs). Identification of factors critical for BCSC function is therefore vital for the development of therapies. Here, we identify the arginine methyltransferase PRMT5 as a key in vitro and in vivo regulator of BCSC proliferation and self-renewal and establish FOXP1, a winged helix/forkhead transcription factor, as a critical effector of PRMT5-induced BCSC function...
December 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/29262320/the-arginine-methyltransferase-prmt6-regulates-dna-methylation-and-contributes-to-global-dna-hypomethylation-in-cancer
#13
Nicolas Veland, Swanand Hardikar, Yi Zhong, Sitaram Gayatri, Jiameng Dan, Brian D Strahl, Scott B Rothbart, Mark T Bedford, Taiping Chen
DNA methylation plays crucial roles in chromatin structure and gene expression. Aberrant DNA methylation patterns, including global hypomethylation and regional hypermethylation, are associated with cancer and implicated in oncogenic events. How DNA methylation is regulated in developmental and cellular processes and dysregulated in cancer is poorly understood. Here, we show that PRMT6, a protein arginine methyltransferase responsible for asymmetric dimethylation of histone H3 arginine 2 (H3R2me2a), negatively regulates DNA methylation and that PRMT6 upregulation contributes to global DNA hypomethylation in cancer...
December 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/29251642/l-arginine-and-arginase-products-potentiate-dexmedetomidine-induced-contractions-in-the-rat-aorta
#14
Emily S W Wong, Ricky Y K Man, Kwok F J Ng, Susan W S Leung, Paul M Vanhoutte
BACKGROUND: The α2-adrenergic sedative/anesthetic agent dexmedetomidine exerts biphasic effects on isolated arteries, causing endothelium-dependent relaxations at concentrations at or below 30 nM, followed by contractions at higher concentrations. L-arginine is a common substrate of endothelial nitric oxide synthase and arginases. This study was designed to investigate the role of L-arginine in modulating the overall vascular response to dexmedetomidine. METHODS: Isometric tension was measured in isolated aortic rings of Sprague Dawley rats...
December 15, 2017: Anesthesiology
https://www.readbyqxmd.com/read/29239694/a-novel-prenyl-polybasic-domain-code-determines-lipid-binding-specificity-of-the-k-ras-membrane-anchor
#15
Yong Zhou, John F Hancock
Ras proteins must localize to the plasma membrane (PM) for biological function. The membrane anchor of the K-Ras4B isoform comprises a farnesylated and methylated C-terminal cysteine together with an adjacent hexa-lysine polybasic domain (PBD). Traditionally, polybasic sequences have been thought to interact electrostatically with negatively charged membranes showing no specificity for anionic lipid head groups. By contrast we recently showed that the K-Ras membrane anchor actually exhibits a very high degree of specificity for phosphatidylserine (PtdSer)...
December 14, 2017: Small GTPases
https://www.readbyqxmd.com/read/29235883/nitric-oxide-modulates-the-responses-of-osteoclast-formation-to-static-magnetic-fields
#16
Jian Zhang, Chong Ding, Xiaofeng Meng, Peng Shang
Nitric oxide (NO) is involved in osteoclast differentiation. Our previous studies showed that static magnetic fields (SMFs) could affect osteoclast differentiation. The inhibitory effects of 16 T of high SMF (HiMF) on osteoclast differentiation was correlated with increased production of NO. We raised the hypothesis that NO mediated the regulatory role of SMFs on osteoclast formation. In this study, 500 nT of hypomagnetic field (HyMF), 0.2 T of moderate SMF (MMF) and 16 T of high SMF (HiMF) were utilized as SMF treatment...
December 13, 2017: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/29234180/role-of-the-nitrergic-system-of-the-cuneiform-nucleus-in-cardiovascular-responses-in-urethane-anesthetized-male-rats
#17
Esmaeil Farrokhi, Mohammad Naser Shafei, Abolfazl Khajavirad, Mahmoud Hosseini, Ali Reza Ebrahimzadeh Bideskan
Background: The presence of nitric oxide (NO) in the cuneiform nucleus (CnF) has been previously shown. In this study, NG-nitro-L-arginine methyl ester (L-NAME) (an inhibitor of NO synthase), L-arginine (L-Arg) (a precursor of NO), and sodium nitroprusside (SNP) (a donor of NO) were microinjected into the CnF and cardiovascular responses were investigated. Methods: Seventy male rats were divided into 7 groups (n=10 each): 1) saline, 2 and 3) L-NAME (30 and 90 nmol), 4 and 5) L-Arg (20 and 60 nmol), and 6 and 7) SNP (9 and 27 nmol)...
September 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29226078/identification-and-in%C3%A2-silico-structural-analysis-of-gallus-gallus-protein-arginine-methyltransferase-4-prmt4
#18
Hannah Berberich, Felix Terwesten, Sinja Rakow, Peeyush Sahu, Caroline Bouchard, Marion Meixner, Sjaak Philipsen, Peter Kolb, Uta-Maria Bauer
Protein arginine methyltransferase 4 (PRMT4) is an essential epigenetic regulator of fundamental and conserved processes during vertebrate development, such as pluripotency and differentiation. Surprisingly, PRMT4 homologs have been identified in nearly all vertebrate classes except the avian genome. This raises the possibility that in birds PRMT4 functions are taken over by other PRMT family members. Here, we reveal the existence of a bona fide PRMT4 homolog in the chicken, Gallus gallus. Using a biochemical approach, we initially purified a putative chicken PRMT4 protein and thus provided the first evidence for the presence of an endogenous PRMT4-specific enzymatic activity toward histone H3 arginine 17 (H3R17) in avian cells...
December 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29225202/epigenetic-modifications-an-important-mechanism-in-diabetic-disturbances
#19
Anna Rorbach-Dolata, Adriana Kubis, Agnieszka Piwowar
In the search for explanations of diabetes pathomechanisms, especially the development of its vascular complications (micro- and macrovascular ), although current, good metabolic control of diabetes, attention was drawn to the role of epigenetic inheritance associated with epigenetic modifications of histone proteins and DNA in hyperglycemia conditions. This study showed the significant role of DNA methylation and histone epigenetic modifications (a different nature and a different degree) in the transmission of information that is not connected with gene inheritance but concerns the persistent changes induced by hyperglycemia...
November 29, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/29222687/chrysophyllum-cainito-apple-star-a-fruit-with-gastroprotective-activity-in-experimental-ulcer-models
#20
Roseane Leandra da Rosa, Camila Leandra de Almeida, Lincon Bordigon Somensi, Thaise Boeing, Luisa Nathália Bolda Mariano, Clarissa de Medeiros Amorim Krueger, Priscila de Souza, Valdir Cechinel Filho, Luisa Mota da Silva, Sérgio Faloni de Andrade
The gastroprotective potential of the methanolic extracts from peels (MEPe), seeds (MESe) and pulp (MEPu) of Chrysophyllum cainito L. (Sapotaceae) fruits was evaluated in mice using ethanol/HCl- and indomethacin-induced ulcer, as well as the antiulcer effect of the juice and flour from this fruit. The lowest oral gastroprotective dose of MEPe, MESe and MEPu against ethanol/HCl was 3, 3 and 10 mg/kg, respectively. Moreover, all extracts increased mucin secretion at 176, 198 and 193%. Intraperitoneal administration of MEPe (0...
December 8, 2017: Inflammopharmacology
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