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https://www.readbyqxmd.com/read/28330192/differential-display-reverse-transcription-pcr-ddrt-pcr-a-new-technology-for-molecular-detection-and-studying-one-of-the-antagonistic-factors-of-bacillus-endophyticus-strain-sa-against-staphylococcus-aureus-mrsa
#1
Mahmoud F Moustafa, Tarek H Taha, M Helal, Sulaiman A Alrumman
Differential Display (DDRT-PCR) is a powerful technique for analyzing differences in gene expression. In-vivo expression technologies and differential display RT-PCR are providing new approaches to further examine a microbe's response to experimental conditions which more closely resemble natural microbial associations and habitats. In this study, Bacillus endophyticus strain SA isolated from the inner tissue of the stem of the cultivated plant (Salvadora persica, Asir, Kingdom of Saudi Arabia) produces an antagonistic factor...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327142/exome-sequencing-identified-rare-variants-in-genes-hspg2-and-atp2b4-in-a-family-segregating-developmental-dysplasia-of-the-hip
#2
Sulman Basit, Alia M Albalawi, Essa Alharby, Khalid I Khoshhal
BACKGROUND: Developmental dysplasia of the hip (DDH) is a common pathological condition of the musculoskeletal system in infants which results in a congenital and developmental malformation of the hip joint. DDH is a spectrum of pathologies affecting the infant hip ranging from asymptomatic subtle radiographic signs through mild instability to frank dislocations with acetabular dysplasia. A Saudi family with three affected individuals with DDH was identified and genetic analysis was performed to detect the possible genetic defect(s) underlying DDH in the affected members of the family...
March 21, 2017: BMC Medical Genetics
https://www.readbyqxmd.com/read/28327109/a-cross-sectional-study-on-sarcopenia-using-different-methods-reference-values-for-healthy-saudi-young-men
#3
Shaea A Alkahtani
BACKGROUND: The aim of this study was to determine reference values for sarcopenia indices using different methods in healthy Saudi young men. METHODS: Participants included 232 Saudi men aged between 20 and 35 years. The study measured anthropometric indices, blood pressure, hand grip strength, and lean muscle mass using dual-energy X-ray absorptiometry (DXA), and bioelectrical impedance analysis (BIA) was performed using Inbody 770 and Tanita 980 devices. RESULTS: Using DXA, the mean value of appendicular lean mass divided by the height squared (ALM/ht(2)) was found to be 8...
March 21, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28326463/relationship-between-selenium-lead-and-mercury-in-red-blood-cells-of-saudi-autistic-children
#4
Afaf El-Ansary, Geir Bjørklund, Alexey A Tinkov, Anatoly V Skalny, Hussain Al Dera
Autism spectrum disorder (ASD) is a neurodevelopmental disorder that can cause significant social, communication and behavioral challenges. Environmental contribution to ASD is due in large part to the sensitivity of the developing brain to external exposures such as lead (Pb), and mercury (Hg) as toxic heavy metals or due to a poor detoxification ability as the phenotype of this disorder. Selenium (Se) as an antioxidant element that counteracts the neurotoxicity of Hg, and Pb, presumably through the formation of nontoxic complexes...
March 21, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28322710/surveillance-and-testing-for-middle-east-respiratory-syndrome-coronavirus-saudi-arabia-april-2015-february-2016
#5
Abdulaziz A Bin Saeed, Glen R Abedi, Abdullah G Alzahrani, Iyad Salameh, Fatima Abdirizak, Raafat Alhakeem, Homoud Algarni, Osman A El Nil, Mutaz Mohammed, Abdullah M Assiri, Hail M Alabdely, John T Watson, Susan I Gerber
Saudi Arabia has reported >80% of the Middle East respiratory syndrome coronavirus (MERS-CoV) cases worldwide. During April 2015-February 2016, Saudi Arabia identified and tested 57,363 persons (18.4/10,000 residents) with suspected MERS-CoV infection; 384 (0.7%) tested positive. Robust, extensive, and timely surveillance is critical for limiting virus transmission.
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28322313/the-epidemic-dynamics-of-hepatitis-c-virus-subtypes-4a-and-4d-in-saudi-arabia
#6
Ahmed A Al-Qahtani, Guy Baele, Nisreen Khalaf, Marc A Suchard, Mashael R Al-Anazi, Ayman A Abdo, Faisal M Sanai, Hamad I Al-Ashgar, Mohammed Q Khan, Mohammed N Al-Ahdal, Philippe Lemey, Bram Vrancken
The relatedness between viral variants sampled at different locations through time can provide information pertinent to public health that cannot readily be obtained through standard surveillance methods. Here, we use virus genetic data to identify the transmission dynamics that drive the hepatitis C virus subtypes 4a (HCV4a) and 4d (HCV4d) epidemics in Saudi Arabia. We use a comprehensive dataset of newly generated and publicly available sequence data to infer the HCV4a and HCV4d evolutionary histories in a Bayesian statistical framework...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316981/growth-profile-and-its-association-with-nutrient-intake-and-dietary-patterns-among-children-and-adolescents-in-hail-region-of-saudi-arabia
#7
Eyad Alshammari, Epuru Suneetha, Mohd Adnan, Saif Khan, Awfa Alazzeh
Hail region of Saudi Arabia (KSA) has the highest adult obesity rates in the entire kingdom and limited information is available about the prevalence and patterns of growth markers. Therefore, it is important to monitor the growth trends to implement effective public health preventive strategies for the region. This study aims to estimate the prevalence of growth profile patterns (stunting, wasting, underweight, and overweight) and its associations with nutrient intake and dietary patterns among children and adolescents...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28316945/a-retrospective-analysis-of-radiographic-jaw-findings-in-young-women-prevalence-and-predictors
#8
Sara M El Khateeb, Osama Abu-Hammad, Hani Fadel, Najla Dar-Odeh
AIMS AND OBJECTIVES: To determine the prevalence and types of jaw pathologic findings as detected in panoramic radiographs of a sample of young women attending a teaching hospital in Al Madinah Al Munawarah, Saudi Arabia, and to determine the most important factors that predict the occurrence of jaw pathologic findings. MATERIALS AND METHODS: The electronic clinical files of a representative sample of female patients who attended the outpatient dental clinics were retrieved...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28304260/the-challenges-of-cultural-competency-among-expatriate-nurses-working-in-kingdom-of-saudi-arabia
#9
Dalyal N Alosaimi, Muayyad M Ahmad
BACKGROUND AND PURPOSE: To explore the cultural challenges facing expatriate nurses working in the Kingdom of Saudi Arabia (KSA). Women do not have an active role in the workplace in KSA. Concepts of religion, culture, and language are considered to be crucial and often the lack of women's participation is attributed to these factors. METHODS: A descriptive qualitative approach was used with two methods of data collection: interviews and focus group. The study was located in a hospital setting in Al-Riyadh at KSA...
November 1, 2016: Research and Theory for Nursing Practice
https://www.readbyqxmd.com/read/28304000/outcome-based-approach-to-medical-education-towards-academic-programmes-accreditation-a-review-article
#10
Abdelmarouf H Mohieldein
The rapid change worldwide, as a consequence of advances in science and technology, necessitates the graduation of well-qualified graduates who have the appropriate knowledge and skills to fulfill specific work requirements. Hence, redesigning academic models by focusing on educational outcomes became the target and priority for universities around the world. In this systematic review we collected and retrieved literature using a selection of electronic databases. The objectives of this report is to: 1) provide an overview of the evolution of outcome-based education (OBE), (2) illustrate the philosophy and principle of OBE, (3) list the OBE advantages and benefits, (4) describe the assessment strategies used in OBE, and (5) discuss the role of teachers and students as key elements...
March 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28303981/obesity-and-associated-risk-factors-among-students-of-health-colleges-of-king-saud-university-saudi-arabia-a-cross-sectional-study
#11
Elshazaly Saeed, Asaad Mohammed Assiri, Ibrahim AwadEljack, Abdullah S Aljasser, Abdulrahman Mohammed Alhuzimi, Ahmed Assad Assiri, Nasser Ali Alqahtani, Saud Amer Alshahrani, Yousif A Al-Ammar
OBJECTIVE: To determine the prevalence of obesity and associated risk factors among medical students in Saudi Arabia. METHODS: The cross-sectional study was conducted from December 2012 to March 2013 at King Saud University, Riyadh, Saudi Arabia. Using stratified sampling technique, undergraduate students of either gender from the health colleges were included. Information was collected through a pretested questionnaire. Measurements of the height and weight were noted and body mass index for all the subjects was calculated...
March 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28302169/two-novel-lhx3-mutations-in-patients-with-combined-pituitary-hormone-deficiency-including-cervical-rigidity-and-sensorineural-hearing-loss
#12
Khushnooda Ramzan, Bassam Bin-Abbas, Lolwa Al-Jomaa, Rabab Allam, Mohammed Al-Owain, Faiqa Imtiaz
BACKGROUND: Congenital combined pituitary hormone deficiency (CPHD) is a rare heterogeneous group of conditions. CPHD-type 3 (CPHD3; MIM# 221750) is caused by recessive mutations in LHX3, a LIM-homeodomain transcription factor gene. The isoforms of LHX3 are critical for pituitary gland formation and specification of the anterior pituitary hormone-secreting cell types. They also play distinct roles in the development of neuroendocrine and auditory systems. CASE PRESENTATION: Here, we summarize the clinical, endocrinological, radiological and molecular features of three patients from two unrelated families...
March 16, 2017: BMC Endocrine Disorders
https://www.readbyqxmd.com/read/28302087/knowledge-and-attitudes-about-vitamin-d-and-behaviors-related-to-vitamin-d-in-adults-with-and-without-coronary-heart-disease-in-saudi-arabia
#13
Najlaa M Aljefree, Patricia Lee, Faruk Ahmed
BACKGROUND: Vitamin D deficiency is prevailing in Saudi Arabia. Recent national data indicated an inverse association between vitamin D status and coronary heart disease (CHD), which increases concerns about vitamin D deficiency as a serious public health problem. Therefore, the current study aimed to investigate whether knowledge, attitudes and behaviors related to vitamin D contribute to the prevalence of vitamin D deficiency among adults with and without CHD in Saudi Arabia. METHODS: This case-control study consisted of 130 CHD cases and 195 matched controls...
March 16, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28299485/characterization-of-lettuce-big-vein-associated-virus-and-mirafiori-lettuce-big-vein-virus-infecting-lettuce-in-saudi-arabia
#14
M Umar, M A Amer, M A Al-Saleh, I M Al-Shahwan, M T Shakeel, A M Zakri, N I Katis
During 2014 and 2015, 97 lettuce plants that showed big-vein-disease-like symptoms and seven weed plants were collected from the Riyadh region. DAS-ELISA revealed that 25% and 9% of the lettuce plants were singly infected with LBVaV and MiLBVV, respectively, whereas 63% had a mixed infection with both viruses. The results were confirmed by multiplex reverse transcription polymerase chain reaction using primers specific for LBVaV and MiLBVV. LBVaV and MiLBVV were also detected in Sonchus oleraceus and Eruca sativa, respectively...
March 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28299214/occupational-neurobrucellosis-mimicking-a-brain-tumor-a-case-report-and-review-of-the-literature
#15
Hussein Algahtani, Bader Shirah, Dina Abdulghani, Roiya Farhan, Raghad Algahtani
Brucellosis is a zoonotic bacterial infection which is transmitted to humans from infected animals and is endemic in many parts of the world including Saudi Arabia. In this article, we report a case of occupational neurobrucellosis that presented with a space-occupying lesion mimicking a brain tumor. We stress on the importance of obtaining detailed social history including occupation to reach the diagnosis in several conditions including brucellosis. We also stress on taking universal precautions when handling any specimens...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28298933/circulation-of-dengue-virus-serotypes-in-the-city-of-makkah-saudi-arabia-as-determined-by-reverse-transcription-polymerase-chain-reaction
#16
Sameer R Organji, Hussein H Abulreesh, Gamal E H Osman
The present study was aimed to investigate the circulation of four dengue virus (DENV) serotypes in Makkah, Western Saudi Arabia. Blood samples were collected from 25 dengue fever-suspected patients and were subjected to molecular typing for DENV-1, DENV-2, DENV-3, and DENV-4 serotypes of dengue virus, by reverse transcription polymerase chain reaction (RT-PCR), using six sets of primers. Of the 25 samples, only six samples (24%) were found to be positive for dengue virus infection. The prevalence of DENV-1 was higher (50% of DENV-positive samples), as compared to DENV-2 (33...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28296806/new-observations-regarding-the-retinopathy-of-genetically-confirmed-kearns-sayre-syndrome
#17
Igor Kozak, Darren T Oystreck, Khaled K Abu-Amero, Sawsan R Nowilaty, Hisham Alkhalidi, Sahar M Elkhamary, Sarar Mohamed, Muddathir H A Hamad, Mustafa A Salih, Emma L Blakely, Robert W Taylor, Thomas M Bosley
PURPOSE: To report novel retinal findings in Kearns-Sayre syndrome and correlate degree of retinopathy with other clinical findings. METHODS: Observational case series of patients from Saudi Arabia with retinal and neuroophthalmologic examinations, medical chart review, and mitochondrial genetic evaluation. RESULTS: The three unrelated patients had progressive external ophthalmoplegia and pigmentary retinopathy bilaterally. Muscle biopsy in two of the cases revealed mitochondrial myopathy...
December 19, 2016: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28296228/coronavirus-infections-in-horses-in-saudi-arabia-and-oman
#18
M G Hemida, D K W Chu, R A P M Perera, R L W Ko, R T Y So, B C Y Ng, S M S Chan, S Chu, A A Alnaeem, M A Alhammadi, R J Webby, L L M Poon, U B R Balasuriya, M Peiris
Equine coronaviruses (ECoV) are the only coronavirus known to infect horses. So far, data on ECoV infection in horses remain limited to the USA, France and Japan and its geographic distribution is not well understood. We carried out RT-PCR on 306 nasal and 315 rectal swabs and tested 243 sera for antibodies to detect coronavirus infections in apparently healthy horses in Saudi Arabia and Oman. We document evidence of infection with ECoV and HKU23 coronavirus by RT-PCR. There was no conclusive evidence of Middle East respiratory syndrome coronavirus infection in horses...
March 13, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28295279/cultural-competence-among-nursing-students-in-saudi-arabia-a-cross-sectional-study
#19
J P Cruz, N Alquwez, C P Cruz, R F D Felicilda-Reynaldo, L M Vitorino, S M S Islam
AIM: This study assessed the cultural competence of nursing students in a Saudi University. BACKGROUND: With the current situation of immigration in Saudi Arabia, the cultural diversity in healthcare facilities is anticipated to grow. This presents a great challenge to the members of the healthcare team. METHODS: A cross-sectional study was conducted among 272 nursing students in a Saudi university using a self-administered questionnaire consisting of two parts, namely the respondents' demographics and cultural background information sheet and the Cultural Capacity Scale Arabic version...
March 13, 2017: International Nursing Review
https://www.readbyqxmd.com/read/28294348/dietary-exposure-to-acrylamide-from-cafeteria-foods-in-jeddah-schools-and-associated-risk-assessment
#20
Mahmoud M Eltawila, Ahmed M Al-Ansari, Amani A Alrasheedi, Abdulateef A Neamatallah
BACKGROUND: Acrylamide (AA) is a carcinogenic and genotoxic food contaminant produced at high temperatures in foods that are rich in carbohydrates. Foods sold in schools in Jeddah, Saudi Arabia, are among such carbohydrate-rich foods produced at high cooking temperatures. It is crucial to determine the importance of AA exposure due to cafeteria foods and to assess the associated risks. RESULTS: The highest mean AA level was measured in chocolate pies (439 µg kg (-1) ), followed by custard pies (435 µg kg (-1) ) and cheese pies (432 µg kg (-1) )...
March 14, 2017: Journal of the Science of Food and Agriculture
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