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https://www.readbyqxmd.com/read/28916427/simultaneous-detection-and-differentiation-of-three-potyviridae-viruses-in-sweet-potato-by-a-multiplex-taqman-real-time-rt-pcr-assay
#1
Pingxiu Lan, Fan Li, Jorge Abad, Lingling Pu, Ruhui Li
A multiplex TaqMan real time RT-PCR was developed for detection and differentiation of Sweet potato virus G, Sweet potato latent virus and Sweet potato mild mottle virus in one tube. Amplification and detection of a fluorogenic cytochrome oxidase gene was included as an internal control. The assay was compared with a multiplex RT-PCR developed in the initial study for the detection and differentiation of the three viruses and host 18S rRNA. Primers and/or probes of the two assays were designed from conserved regions of each virus...
September 12, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28916301/enhancing-monoterpene-alcohols-in-sweet-potato-shochu-using-the-diglycoside-specific-%C3%AE-primeverosidase
#2
Yuichiro Sato, Jinshun Han, Hisashi Fukuda, Shigeaki Mikami
Monoterpene alcohols (MTAs) are characteristic flavour-imparting compounds in sweet potato shochu (Japanese distilled spirit) that are liberated following hydrolysis by specific enzymes during fermentation. In the present study, we evaluated the effect of an exogenously added diglycoside-specific β-glycosidase (β-primeverosidase) on aroma formation during shochu brewing using various sweet potato species to address whether MTAs are predominantly present as diglycosidic precursors in raw materials. The results showed that the amount of MTAs produced from enzyme-treated mash was dramatically increased by 2- to 9-fold compared with untreated controls, and the increase varied with sweet potato species...
September 12, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28911647/characterization-and-hepatoprotective-activity-of-anthocyanins-from-purple-sweet-potato-ipomoea-batatas-l-cultivar-eshu-no-8
#3
Lin Wang, Ying Zhao, Qing Zhou, Chun-Li Luo, Ai-Ping Deng, Zi-Cheng Zhang, Jiu-Liang Zhang
The hepatoprotective activity of anthocyanin-rich purple sweet potato extract (APSPE) was demonstrated. Sixty mice were randomly divided into six groups: control group [without carbon tetrachloride (CCl4) or APSPE]; model group (with CCl4 only); positive control group (50 mg/kg body weight silymarin); low-dose group (100 mg/kg body weight APSPE); medium-dose group (200 mg/kg body weight APSPE); and high-dose group (400 mg/kg body weight APSPE). After 10 days intragastric administration of the respective supplements, the mice in all groups except control were injected intraperitoneally with CCl4 (0...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28888446/changes-in-anthocyanins-and-volatile-components-of-purple-sweet-potato-fermented-alcoholic-beverage-during-aging
#4
Suhong Li, Yingfeng An, Weina Fu, Xiao Sun, Wenjie Li, Tuoping Li
Purple sweet potato was fermented into alcoholic beverage. By using LC-MS analysis, 12 types of anthocyanins were found in the purple sweet potato alcoholic beverage (PSPFAB); these were based on cyanidin and peonidin as aglycones. The anthocyanins in young PSPFAB mainly consisted of acylates. The acylated anthocyanins, however, degraded gradually with aging. Cyanidin 3-sophoroside-5-glucoside and peonidin 3-sophoroside-5-glucoside were found to be major anthocyanins in the PSPFAB after two years of aging. Moreover, 52 kinds of volatile components were detected in PSPFAB by GC-MS analysis...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28870255/detection-and-epidemic-dynamic-of-tocv-and-ccyv-with-bemisia-tabaci-and-weed-in-hainan-of-china
#5
Xin Tang, Xiaobin Shi, Deyong Zhang, Fan Li, Fei Yan, Youjun Zhang, Yong Liu, Xuguo Zhou
BACKGROUND: In recent years, two of the crinivirus, Tomato chlorosis virus (ToCV) and Cucurbit chlorotic yellows virus (CCYV) have gained increasing attention due to their rapid spread and devastating impacts on vegetable production worldwide. Both of these viruses are transmitted by the sweet potato whitefly, Bemisia tabaci (Gennadius), in a semi-persistent manner. Up to now, there is still lack of report in Hainan, the south of China. METHODS: We used observational and experimental methods to explore the prevalence and incidence dynamic of CCYV and ToCV transmitted by whiteflies in Hainan of China...
September 4, 2017: Virology Journal
https://www.readbyqxmd.com/read/28852001/salt-tolerance-response-revealed-by-rna-seq-in-a-diploid-halophytic-wild-relative-of-sweet-potato
#6
Yan Luo, Robert Reid, Daniella Freese, Changbao Li, Jonathan Watkins, Huazhong Shi, Hengyou Zhang, Ann Loraine, Bao-Hua Song
Crop wild relatives harbor exotic and novel genetic resources, which hold great potential for crop improvement. Ipomoea imperati is a wild diploid relative of sweet potato with the capability of high salinity tolerance. We compared the transcriptomes of I. imperati under salt stress vs. control to identify candidate genes and pathways involved in salt response. De novo assembly produced 67,911 transcripts with a high depth of coverage. A total of 39,902 putative genes were assigned annotations, and 936 and 220 genes involved in salt response in roots and leaves, respectively...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28839380/optimization-of-polysaccharide-ultrasonic-extraction-conditions-using-purple-sweet-potato-tubers-based-on-free-radical-scavenging-and-glycosylation-inhibitory-bioactivities
#7
Haihua Guo, Fansheng Kong, Chunyan Yan
BACKGROUND: The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE: To predict the ultrasonic conditions for getting the optimal in vitro antioxidant and antiglycated activity of ultrasonic extracted polysaccharides from purple sweet potato (I. batatas) tubers, the artificial neural network (ANN) regression models was used in this study...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28827752/haplotype-resolved-sweet-potato-genome-traces-back-its-hexaploidization-history
#8
Jun Yang, M-Hossein Moeinzadeh, Heiner Kuhl, Johannes Helmuth, Peng Xiao, Stefan Haas, Guiling Liu, Jianli Zheng, Zhe Sun, Weijuan Fan, Gaifang Deng, Hongxia Wang, Fenhong Hu, Shanshan Zhao, Alisdair R Fernie, Stefan Boerno, Bernd Timmermann, Peng Zhang, Martin Vingron
Here we present the 15 pseudochromosomes of sweet potato, Ipomoea batatas, the seventh most important crop in the world and the fourth most significant in China. By using a novel haplotyping method based on genome assembly, we have produced a half haplotype-resolved genome from ~296 Gb of paired-end sequence reads amounting to roughly 67-fold coverage. By phylogenetic tree analysis of homologous chromosomes, it was possible to estimate the time of two recent whole-genome duplication events as occurring about 0...
August 21, 2017: Nature Plants
https://www.readbyqxmd.com/read/28827641/disentangling-a-polyploid-genome
#9
Haibao Tang
The sequencing and draft assembly of the sweet potato genome has resolved much of its complex genetic redundancies, allowing a better inference of the botanical origin of the staple crop.
August 21, 2017: Nature Plants
https://www.readbyqxmd.com/read/28811113/acrylamide-forming-potential-of-cereals-legumes-and-roots-and-tubers-analyzed-by-uplc-uv
#10
Joseph H Y Galani, Nilesh J Patel, Jayant G Talati
For directing scientists, consumers, industry and stakeholders on mitigation strategies, there is a need to understand the acrylamide-forming potential of important Indian foods. Flour obtained from total 16 varieties of 9 Indian cereals, legumes and roots and tubers was heated at 160 °C for 20 min, acrylamide was extracted and quantified by UPLC-UV. Acrylamide level was above the European Commission indicative value in potato- and cereal-based food products, it ranged from 3436.13 to 5562.56 μg/kg in roots and tubers (potato and sweet potato)...
August 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28804701/application-of-methanol-and-sweet-potato-vine-hydrolysate-as-enhancers-of-citric-acid-production-by-aspergillus-niger
#11
Daobing Yu, Yanke Shi, Qun Wang, Xin Zhang, Yuhua Zhao
BACKGROUND: Agricultural waste is as an alternative low-cost carbon source or beneficial additives which catch most people's eyes. In addition, methanol and sweet potato vine hydrolysate (SVH) have been reported as the efficient enhancers of fermentation according to some reports. The objective of the present study was to confirm SVH as an efficient additive in CA production and explore the synergistic effects of methanol and SVH in fermentation reactions. RESULTS: The optimal fermentation conditions resulted in a maximum citric acid concentration of 3...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28791000/starch-flocculation-by-the-sweet-potato-sour-liquid-is-mediated-by-the-adhesion-of-lactic-acid-bacteria-to-starch
#12
Lili Zhang, Yang Yu, Xinhua Li, Xiaona Li, Huajiang Zhang, Zhen Zhang, Yunhe Xu
In the current study, we focused on the mechanism underlying starch flocculation by the sweet potato sour liquid. The traditional microbial techniques and 16S rDNA sequencing revealed that Lactobacillus was dominant flocculating microorganism in sour liquid. In total, 86 bacteria, 20 yeasts, and 10 molds were isolated from the sour liquid and only eight Lactobacillus species exhibited flocculating activity. Lactobacillus paracasei subsp. paracasei L1 strain with a high flocculating activity was isolated and identified, and the mechanism of starch flocculation was examined...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28780262/production-of-poly-%C3%AE-l-malic-acid-by-aureobasidium-pullulans-ha-4d-under-solid-state-fermentation
#13
Jun Xia, Rongqing Li, Aiyong He, Jiaxing Xu, Xiaoyan Liu, Xiangqian Li, Jiming Xu
Poly(β-l-malic acid) (PMA) production by Aureobasidium pullulans HA-4D was carried out through solid-state fermentation (SSF) using agro-industrial residues. Maximum PMA production (75.4mg/g substrate) was obtained from a mixed substrate of sweet potato residue and wheat bran (1:1, w/w) supplemented with NaNO3 (0.8%, w/w) and CaCO3 (2%, w/w), with an initial moisture content of 70% and inoculum size of 13% (v/w) for 8days. Repeated-batch SSF was successfully conducted for 5 cycles with a high productivity...
July 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28776446/vegetable-relishes-high-in-%C3%AE-carotene-increase-the-iron-zinc-and-%C3%AE-carotene-nutritive-values-from-cereal-porridges
#14
Johanita Kruger, Annelies Breynaert, Luc Pieters, Nina Hermans
Iron, zinc and vitamin A deficiencies are serious public health problems in sub-Saharan Africa, which can be alleviated by dietary diversification. The effects of adding cowpea leaf (CL) and orange-fleshed sweet potato (OFSP) relishes to sorghum and maize porridges on iron, zinc and β-carotene contents and bioaccessibilities were determined. Despite the high iron content of the CL relish (14.59 mg/100 g), the vegetable relishes had little effect on the iron bioaccessibility from the cereal porridges. Importantly, the addition of the CL relish increased the percentage and amount of bioaccessible zinc 2- and 3-fold, respectively...
August 4, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28764038/dietary-acrylamide-what-happens-during-digestion
#15
M Sansano, A Heredia, I Peinado, A Andrés
Acrylamide is a well-known potentially carcinogen compound formed during thermal processing as an intermediate of Maillard reactions. Three objectives were addressed: the impact of gastric digestion on acrylamide content of French Fries, chips, chicken nuggets, onions rings, breakfast cereals, biscuits, crackers, instant coffee and coffee substitute; the acrylamide content evolution during gastrointestinal digestion of French fries and chips; and the effectiveness of blanching and air-frying on acrylamide mitigation after gastrointestinal digestion...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28761111/use-of-c4mim-cl-for-efficient-extraction-of-caffeoylquinic-acids-from-sweet-potato-leaves
#16
Toyonobu Usuki, Shingo Onda, Masahiro Yoshizawa-Fujita, Masahiro Rikukawa
Sweet potato, Ipomoea batatas, is a widely cultivated vegetable worldwide. The leaves contain polyphenolic natural products called caffeoylquinic acids (CQAs), which possess biological activities including inhibition of aggregation of amyloid peptides. The present study describes an efficient extraction and isolation procedure for CQAs from sweet potato leaves using a cellulose-dissolving ionic liquid. The results showed that, compared to methanol, use of 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) allowed the extraction of a 6...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28758207/the-potential-of-computer-vision-optical-backscattering-parameters-and-artificial-neural-network-modelling-in-monitoring-the-shrinkage-of-sweet-potato-ipomoea-batatas-l-during-drying
#17
Daniel I Onwude, Norhashila Hashim, Khalina Abdan, Rimfiel Janius, Guangnan Chen
BACKGROUND: Drying is a method used to preserve agricultural crops. During the drying of products with high moisture content, structural changes in shape, volume, area, density and porosity occur. This changes could affect the final quality of dried product and also the effective design of drying equipment. Therefore, this study investigated a novel approach in monitoring and predicting the shrinkage of sweet potato during drying. Drying experiments were conducted at temperatures of 50-70 °C and samples thicknesses of 2 - 6 mm...
July 30, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28739411/impact-of-heat-moisture-and-annealing-treatments-on-physicochemical-properties-and-digestibility-of-starches-from-different-colored-sweet-potato-varieties
#18
Phan Thanh Bao Trung, Luu Bui Bao Ngoc, Phan Ngoc Hoa, Nguyen Ngoc Thanh Tien, Pham Van Hung
The objective of this study is to investigate the change in physicochemical properties and digestibility of starches isolated from colored sweet potato varieties under heat-moisture treatment (HMT) or annealing treatment (ANN). The results showed that morphology and X-ray diffraction patterns of the sweet potato starches remained unchanged after the HMT or ANN. The HMT significantly reduced peak viscosity, breakdown and setback and significantly increased pasting temperature, trough and final viscosities of the sweet potato starches...
July 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28737681/nutrient-and-total-polyphenol-contents-of-dark-green-leafy-vegetables-and-estimation-of-their-iron-bioaccessibility-using-the-in-vitro-digestion-caco-2-cell-model
#19
Francis Kweku Amagloh, Richard Atinpoore Atuna, Richard McBride, Edward Ewing Carey, Tatiana Christides
Dark green leafy vegetables (DGLVs) are considered as important sources of iron and vitamin A. However, iron concentration may not indicate bioaccessibility. The objectives of this study were to compare the nutrient content and iron bioaccessibility of five sweet potato cultivars, including three orange-fleshed types, with other commonly consumed DGLVs in Ghana: cocoyam, corchorus, baobab, kenaf and moringa, using the in vitro digestion/Caco-2 cell model. Moringa had the highest numbers of iron absorption enhancers on an "as-would-be-eaten" basis, β-carotene (14169 μg/100 g; p < 0...
July 22, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28728684/healthful-and-unhealthful-plant-based-diets-and-the-risk-of-coronary-heart%C3%A2-disease-in-u-s-adults
#20
Ambika Satija, Shilpa N Bhupathiraju, Donna Spiegelman, Stephanie E Chiuve, JoAnn E Manson, Walter Willett, Kathryn M Rexrode, Eric B Rimm, Frank B Hu
BACKGROUND: Plant-based diets are recommended for coronary heart disease (CHD) prevention. However, not all plant foods are necessarily beneficial for health. OBJECTIVES: This study sought to examine associations between plant-based diet indices and CHD incidence. METHODS: We included 73,710 women in NHS (Nurses' Health Study) (1984 to 2012), 92,329 women in NHS2 (1991 to 2013), and 43,259 men in Health Professionals Follow-up Study (1986 to 2012), free of chronic diseases at baseline...
July 25, 2017: Journal of the American College of Cardiology
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