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1,25(OH)2D3

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https://www.readbyqxmd.com/read/28624282/effect-of-endoplasmic-reticulum-stress-on-metabolic-and-stress-signaling-and-kidney-specific-functions-in-madin-darby-bovine-kidney-cells
#1
E Chiappisi, R Ringseis, K Eder, D K Gessner
Recent studies demonstrated induction of endoplasmic reticulum (ER) stress in tissues of cows after parturition, but knowledge about the effect of ER stress on important cellular processes, such as critical signaling and metabolic pathways, in cattle is scarce. Thus, the present study aimed to investigate the effect of ER stress induction on nuclear factor-κB (NF-κB), nuclear factor E2-related factor 2 (Nrf2), and sterol regulatory element-binding protein (SREBF1) pathway in Madin-Darby bovine kidney (MDBK) cells, a widely used in vitro model in ruminant research...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28602960/the-impact-of-vdr-expression-and-regulation-in-vivo
#2
Seong Min Lee, Mark B Meyer, Nancy A Benkusky, Charles A O'Brien, J Wesley Pike
The vitamin D receptor (VDR) mediates the pleiotropic biological actions of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). These actions include orchestration of mineral homeostasis which is coordinated by the kidney, intestine, bone and parathyroid gland wherein the VDR transcriptionally regulates expression of the genes involved in this complex process. Mutations in human VDR (hVDR) cause hereditary vitamin D resistant rickets, a genetic syndrome characterized by hypocalcemia, hyperparathyroidism and rickets resulting from dysregulation of mineral homeostasis...
June 8, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28591703/vitamin-d-receptor-activation-reduces-vcap-xenograft-tumor-growth-and-counteracts-erg-activity-despite-induction-of-tmprss2-erg
#3
Justin M Roberts, Rebeca San Martin, D Badrajee Piyarathna, James G MacKrell, Guilherme V Rocha, Jeffery A Dodge, Cristian Coarfa, Venkatesh Krishnan, David R Rowley, Nancy L Weigel
Whether vitamin D is chemopreventive and/or has potential therapeutically in prostate cancer is unresolved. One confounding factor is that many prostate cancers express a TMPRSS2:ERG fusion gene whose expression is increased both by androgens and by vitamin D receptor (VDR) activation. Two challenges that limit VDR agonist use clinically are hypercalcemia and the cooperation of VDR with ERG to hyper-induce the 1α,25-dihydroxyvitamin D3 metabolizing enzyme, CYP24A1, thus reducing VDR activity. Using the VCaP TMPRSS2:ERG positive cell line as a model, we found that a nonsecosteroidal CYP24A1 resistant VDR agonist, VDRM2, substantially reduces growth of xenograft tumors without inducing hypercalcemia...
May 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28587998/clinical-and-genetic-analysis-of-two-chinese-families-with-vitamin-d-dependent-rickets-type-ia-and-follow-up
#4
Chen Ruimin, Li Yunfei, Yuan Xin, Lin Xiangquan, Yang Xiaohong, Zhang Ying
OBJECTIVE: Vitamin D-dependent rickets type IA (VDDR-IA) is a rare autosomal recessive disorder characterized by the early onset of severe rickets. The objectives of this study were twofold: 1) to analyze the clinical characteristics and therapy of two patients with VDDR-IA from two separate Chinese family, and 2) investigate the CYP27B1 gene in two large pedigrees. METHODS: Medical history, clinical manifestations, physical examination, radiological findings and laboratory data were analyzed from two patients with VDDR-IA...
June 7, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28579120/vitamin-d-and-the-brain-genomic-and-non-genomic-actions
#5
REVIEW
Xiaoying Cui, Helen Gooch, Alice Petty, John J McGrath, Darryl Eyles
1,25(OH)2D3 (vitamin D) is well-recognized as a neurosteroid that modulates multiple brain functions. A growing body of evidence indicates that vitamin D plays a pivotal role in brain development, neurotransmission, neuroprotection and immunomodulation. However, the precise molecular mechanisms by which vitamin D exerts these functions in the brain is still unclear. Vitamin D signalling occurs via the vitamin D receptor (VDR), a zinc-finger protein in the nuclear receptor superfamily. Like other nuclear steroids, vitamin D has both genomic and non-genomic actions...
June 1, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28578001/1%C3%AE-25-dihydroxyvitamin-d3-enhances-trpv6-transcription-through-p38-mapk-activation-and-gadd45-expression
#6
Michiyasu Ishizawa, Daisuke Akagi, Jumpei Yamamoto, Makoto Makishima
The active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], acts as a ligand for the vitamin D receptor (VDR), and regulates various physiological processes, including calcium and bone metabolism, cellular growth and differentiation, immunity and cardiovascular function. A number of vitamin D derivatives have been synthesized for the treatment of cancer and inflammatory disease, but the adverse effect of hypercalcemic activity due to intestinal calcium absorption has limited wide clinical application...
May 31, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28565795/1-25-oh-2d3-and-its-analogue-bxl-628-inhibit-high-glucose-induced-activation-of-rhoa-rock-pathway-in-hk-2-cells
#7
Wei Zhang, Bin Yi, Ke Zhang, Aimei Li, Shikun Yang, Jing Huang, Jishi Liu, Hao Zhang
It has previously been reported that 1,25-(OH)2D3 inhibits high glucose-induced epithelial-to-mesenchymal transition (EMT) in HK-2 cells. However, the mechanism of this renoprotective action remains unclear. Elocalcitol (BXL-628), a vitamin D analog, has been suggested to be effective on the RhoA/Rho associated protein kinase (ROCK) pathway, which serves a crucial role in high glucose-induced EMT. The aim of the present study was to investigate the effect of 1,25-(OH)2D3 and its analogue BXL-628 on high glucose-induced activation of the RhoA/ROCK pathway in human renal proximal tubular cells...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28552332/1-25-dihydroxyvitamin-d3-suppressed-experimental-autoimmune-encephalomyelitis-through-both-immunomodulation-and-oligodendrocyte-maturation
#8
Hasti Atashi Shirazi, Javad Rasouli, Bogoljub Ciric, Danmeng Wei, Abdolmohamad Rostami, Guang-Xian Zhang
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has recently been found to have the anti-inflammatory potential to suppress experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis; however, its direct effect on neural cells is not clear. In the current study we show that 1,25(OH)2D3 treatment effectively suppressed clinical signs of ongoing EAE and reduced inflammation and demyelination scores in the central nervous system (CNS). The treatment significantly decreased production/expression of pro-inflammatory cytokines IFN-γ, GM-CSF and IL-17A, while it increased anti-inflammatory cytokines IL-4 and IL-10...
May 25, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28549199/effect-of-1-25-oh2d3-on-the-proliferation-of-human-mesangial-cells-and-their-expression-of-ki67
#9
D Zhao, C-J Zhang, R Yang, J-P Chen, L Ma, G Liu, X-P Yang
Previous studies have found that 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 or VD3] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)2D3 on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and to establish its mechanism of action. Cultured human mesangial cells were randomly divided into the following four groups: normal control (N group; administered Dulbecco's modified Eagle's medium containing 5% fetal bovine serum), proliferation [epidermal growth factor (EGF) group; administered 10 μg/L EGF], VD3 intervention [administered 10(-8) M 1,25(OH)2D3], and proliferation and intervention [EGF+VD3 group; administered 10 μg/L EGF and 10(-8) M 1,25(OH)2D3]...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28540351/cytokine-data-obtained-from-synovial-stromal-cells-of-patients-with-rheumatoid-arthritis-or-osteoarthritis
#10
Johanna A Huhtakangas, Johanna Veijola, Sanna Turunen, Anna Karjalainen, Maarit Valkealahti, Tomi Nousiainen, Susanna Yli-Luukko, Olli Vuolteenaho, Petri Lehenkari
In this article, we share the raw cytokine data obtained from basal and stimulated synovial stromal cells cultured from patients with rheumatoid arthritis or osteoarthritis. This data article is related to the research article entitled "1,25D3 and calcipotriol, its hypocalcemic analog, exert a long-lasting anti-inflammatory and anti-proliferative effect in synoviocytes cultured from patients with rheumatoid arthritis and osteoarthritis (1). Cytokine levels were analyzed by a magnetic bead-based multiplex assay (a panel of 27 important cytokines) in two separate sets of experiments...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28539530/active-form-of-vitamin-d-ameliorates-non-alcoholic-fatty-liver-disease-by-alleviating-oxidative-stress-in-a-high-fat-diet-rat-model
#11
Chong-Gui Zhu, Ya-Xin Liu, Hao Wang, Bao-Ping Wang, Hui-Qi Qu, Bao-Li Wang, Mei Zhu
The purpose of this study was to determine whether treatment using the active form of vitamin D (1,25(OH)2D3) could protect against high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in rats and ameliorate oxidative stress. Male Sprague-Dawley rats were divided into three groups and treated with standard chow, HFD, or HFD plus intraperitoneal injection of 1,25(OH)2D3 (5 μg/kg body weight, twice per week), respectively, for 16 weeks. Serum lipid profiles, hepatic function, intrahepatic lipid, and calcium levels were determined...
May 23, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28536085/interaction-between-the-effects-of-the-selective-estrogen-modulator-femarelle-and-a-vitamin-d-analog-in-human-umbilical-artery-vascular-smooth-muscle-cells
#12
Dalia Somjen, Esther Knoll, Orli Sharon, Ariel Many, Naftali Stern
To further investigate the interaction between vitamin D system and estrogen-mimetic compounds in the human vasculature we studied the effect of the "less- calcemic" analog of 1,25(OH)2D3 (1,25D); JK 1624F2-2 (JKF) in the presence of selective estrogen modulator femarelle (F), the phytoestrogen daidzein (D) and estradiol-17b (E2) on (3)[H] thymidine incorporation (DNA synthesis) and creatine kinase specific activity (CK) in human umbilical artery vascular smooth muscle cells (VSMC). F, D and E2, stimulated DNA synthesis at low concentrations, and inhibited it at high concentrations...
May 20, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28528333/effect-of-1-25-oh-2d3-on-proliferation-of-fibroblast-like-synoviocytes-and-expressions-of-pro-inflammatory-cytokines-through-regulating-microrna-22-in-a-rat-model-of-rheumatoid-arthritis
#13
Ping Fan, Lan He, Nan Hu, Jing Luo, Jing Zhang, Ling-Fei Mo, Yan-Hua Wang, Dan Pu, Xiao-Hong Lv, Zhi-Ming Hao, Chang-Hai Ding, Wu-Jun Xue, Yang Li
OBJECTIVE: This study aims to investigate the regulatory mechanism of 1,25-(OH)2D3 on the proliferation of fibroblast-like synoviocytes (FLS) and expressions of pro-inflammatory cytokines in rheumatoid arthritis (RA) rats via microRNA-22 (miR-22). METHODS: A rat model of RA was established with a subcutaneous injection of type II collagen. After treated with different concentrations of 1,25-(OH)2D3 the proliferation of FLS was estimated by the MTT method, and the optimal concentration of 1,25-(OH)2D3 was selected for further experiments...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28526240/endocrine-actions-of-vitamin-d-in-skin-relevance-for-photocarcinogenesis-of-non-melanoma-skin-cancer-and-beyond
#14
REVIEW
Jörg Reichrath, Roman Saternus, Thomas Vogt
The skin represents a pivotal organ for the human body's vitamin D endocrine system, being both the site of ultraviolet (UV)-B-induced vitamin D synthesis and a target tissue for the pluripotent effects of 1,25(OH)2D3 and other biologically active vitamin D metabolites. As many other steroid hormones, 1,25(OH)2D3 exerts its effects via two independent signal transduction pathways: the classical genomic and the non-genomic pathway. While non-genomic effects of 1,25(OH)2D3 are in part exerted via effects on intracellular calcium, genomic effects are mediated by the vitamin D receptor (VDR)...
May 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28509123/serum-calcitriol-levels-in-a-patient-with-x-linked-hypophosphatemia-complicated-by-autosomal-dominant-polycystic-kidney-disease
#15
Satomi Kajita, Takehisa Yamamoto, Naoko Tsugawa, Hirohumi Nakayama, Takuo Kubota, Toshimi Michigami, Keiichi Ozono
Serum calcitriol [1,25(OH)2D] levels are low normal in the presence of hypophosphatemia in X-linked dominant hypophosphatemic rickets (XLH) due to elevated serum fibroblast growth factor 23 (FGF23) levels. We report a peculiar finding of markedly elevated serum 1,25(OH)2D levels in a patient with XLH complicated by autosomal dominant polycystic kidney disease (ADPKD) and retinitis pigmentosa (RP). She was diagnosed with XLH, ADPKD and RP at the age of 5, 13 and 15 years, respectively. After the diagnosis of ADPKD, the 1,25(OH)2D levels increased up to 282 pg/mL treated with a pharmacological dose of 1αOHD3 (1αOHD3)...
May 2017: CEN Case Reports
https://www.readbyqxmd.com/read/28506446/serum-24-25-dihydroxyvitamin-d3-response-to-native-vitamin-d2%C3%A2-and%C3%A2-d3-supplementation-in-patients-with-chronic-kidney-disease%C3%A2-on%C3%A2-hemodialysis
#16
Laura A Graeff-Armas, Martin Kaufmann, Elizabeth Lyden, Glenville Jones
BACKGROUND & AIMS: While vitamin D deficiency is common in patients with end stage renal disease on dialysis and treatment with Vitamin D2 and Vitamin D3 is becoming increasingly common in these patients, little is known about 24,25(OH)2D3 metabolite production. Some authors report that the CYP24A1 enzyme is upregulated in CKD, but reports of low serum levels of 24,25(OH)2D3 in these patients bring this into question. Lack of substrate or increased clearance of the metabolite have been proposed as possible causes...
April 30, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28476406/influence-of-cdx2-and-taqi-gene-variants-on-vitamin-d3-modulated-intracellular-chemokine-positive-t-cell-subsets-in-pulmonary-tuberculosis
#17
Murugesan Harishankar, Paramasivam Selvaraj
PURPOSE: We studied the effect of 1,25(OH)2D3 (vitamin D3) on intracellular chemokine-positive T-cell subsets in whole blood cultures of healthy controls and patients with pulmonary tuberculosis. METHODS: Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism method. The regulatory role of the Cdx2 and 3'UTR TaqI gene variants on chemokine-positive T-cell subsets was studied from culture filtrate antigen stimulated with or without vitamin D3 treated whole blood cultures of 60 healthy controls and 50 patients with pulmonary tuberculosis...
May 2, 2017: Clinical Therapeutics
https://www.readbyqxmd.com/read/28465245/1-25-dihydroxyvitamin-d3-suppresses-gastric-cancer-cell-growth-through-vdr-and-mutant-p53-mediated-induction-of-p21
#18
Mingxing Li, Longfei Li, Lin Zhang, Wei Hu, Jing Shen, Zhangang Xiao, Xu Wu, Franky Leung Chan, Chi Hin Cho
AIMS: Previous studies have indicated that vitamin D deficiency correlates with cancer risk and vitamin D potentiates antitumor effects in a variety of cancers. The antitumor effect of vitamin D on gastric cancer was rarely studied. We aimed to investigate the antitumor effect of vitamin D on gastric cancer and underlying mechanisms. MAIN METHODS: We investigated the antitumor activity of the active form of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) on gastric cancer cells (TMK1) and immortalized normal gastric cells (HFE145) by using MTT, colony formation and Flow cytometry assays...
April 29, 2017: Life Sciences
https://www.readbyqxmd.com/read/28463086/vitamin-d-counteracts-an-il-23-dependent-il-17a-ifn%C3%AE-response-driven-by-urban-particulate-matter
#19
Elizabeth H Mann, Tzer-Ren Ho, Paul E Pfeffer, Nick C Matthews, Elfy Chevretton, Ian Mudway, Frank J Kelly, Catherine M Hawrylowicz
Urban particulate matter (UPM) air pollution and vitamin D deficiency are detrimentally associated with respiratory health; this is hypothesised to be due in part to regulation of IL-17A, which UPM is reported to promote. Here we use a myeloid (m)DC-memory CD4+ T cell co-culture system to characterize UPM-driven IL-17A+ cells, investigate the mechanism by which UPM-primed DCs promote this phenotype and address evidence for cross-regulation by vitamin D. CD1c+ mDCs were cultured overnight with or without a reference source of UPM and/or active vitamin D (1,25(OH)2D3) before co-culturing with autologous memory CD4+ T cells...
May 2, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28455490/1-25-oh-2d3-protects-liver-fibrosis-through-decreasing-the-generation-of-th17-cells
#20
Lei Gu, Qing Xu, Hui Cao
BACKGROUND The aim of this study was to study the effects of 1-alpha,25-dihydroxy-cholecalcifero (1,25(OH)2D3) on liver fibrosis and the generation of Th17 cells in vivo and in vitro. MATERIAL AND METHODS Thirty C57 mice were randomly divided into control, model, and treatment groups. Hepatic fibrosis was induced by subcutaneous injection of CCl4. Liver fibrosis condition was evaluated through pathological inspection and blood biochemical examination of liver function. Immunohistochemical assays were used to detect the expression of α-SMA, TGF-β, and collagen I to observe hepatic stellate cell activation level...
April 29, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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