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1,25(OH)2D3

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https://www.readbyqxmd.com/read/29026610/selective-blood-sampling-for-fgf-23-in-tumor-induced-osteomalacia
#1
Hans-Christof Schober, Christian Kneitz, Franziska Fieber, Kathrin Hesse, Henry Schroeder
Tumor-induced osteomalacia (TIO) is caused by the hormone fibroblast growth factor 23 (FGF-23). It is mainly produced in the tissue of mesenchymal tumors. Patients with TIO frequently suffer from a chronic decompensated pain syndrome and/or muscle weakness with postural deformity. Despite the severity of the disease, the diagnosis is frequently established late. In some cases, it takes several years to establish the condition. This case report concerning a 68-year old woman demonstrates the selective blood sampling for FGF-23 as path-breaking diagnostics to confirm the diagnosis of a neuroendocrine tumor...
2017: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/29021285/placental-vitamin-d-metabolism-and-its-associations-with-circulating-vitamin-d-metabolites-in-pregnant-women
#2
Heyjun Park, Madeleine R Wood, Olga V Malysheva, Sara Jones, Saurabh Mehta, Patsy M Brannon, Marie A Caudill
Background: Little is known about placental vitamin D metabolism and its impact on maternal circulating vitamin D concentrations in humans.Objective: This study sought to advance the current understanding of placental vitamin D metabolism and its role in modulating maternal circulating vitamin D metabolites during pregnancy.Design: Nested within a feeding study, 24 healthy pregnant women (26-29 wk of gestation) consumed a single amount of vitamin D (511 IU/d from diet and a cholecalciferol supplement) for 10 wk...
October 11, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29018141/vitamin-d-regulation-of-gdnf-ret-signaling-in-dopaminergic-neurons
#3
Renata A N Pertile, Xiaoying Cui, Luke Hammond, Darryl W Eyles
1,25(OH)2D3 (vitamin D) appears essential for the normal development of dopaminergic neurons. Vitamin D affects dopamine synthesis and metabolism as well as expression of glial cell line-derived neurotrophic factor (GDNF), which is crucial for the survival of dopaminergic neurons. We investigated the role of vitamin D on GDNF and its receptors protooncogene tyrosine-protein kinase receptor Ret (C-Ret) and GDNF family receptor alpha 1 (GFRα1) signaling. To this end, we used a developmental vitamin D-deficient rat model and SH-SY5Y cells transfected with vitamin D receptor (VDR)...
October 10, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28959253/methylation-of-the-vitamin-d-receptor-vdr-gene-together-with-genetic-variation-race-and-environment-influence-the-signaling-efficacy-of-the-toll-like-receptor-2-1-vdr-pathway
#4
Vanessa Meyer, Donovan Sean Saccone, Fidele Tugizimana, Furaha Florence Asani, Tamsyn Jacki Jeffery, Liza Bornman
BACKGROUND: The disparity in prevalence of infectious diseases across the globe is common knowledge. Vitamin D receptor (VDR)-mediated toll-like receptor (TLR) 2/1 signaling produces antimicrobial peptides, which is critical as a first line of defense in innate immunity. Numerous studies disclosed the independent role of genetic polymorphisms in this pathway, vitamin D status or season and more recently epigenetics, as factors contributing to infectious disease predisposition. Few studies address the interaction between environment, genetics, and epigenetics...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28941998/vitamin-d3-supplementation-decreases-a-unique-circulating-monocyte-cholesterol-pool-in-patients-with-type-2-diabetes
#5
Amy E Riek, Jisu Oh, Isra Darwech, Veronica Worthy, Xiaobo Lin, Richard E Ostlund, Rong M Zhang, Carlos Bernal-Mizrachi
Cross-sectional studies indicate consistent associations between low 25(OH)D concentration and increased risk of cardiovascular disease (CVD), but results of randomized control trials (RCTs) are mixed. However, the majority of the RCTs do not focus on type 2 diabetics, potentially obscuring the effects of vitamin D in this population. In vitro 1,25(OH)2D3 downregulates macrophage cholesterol deposition, but the in vivo effects are unknown. To explore potential mechanisms of the effects of vitamin D on CVD risk in patients with type 2 diabetes, we isolated monocytes in a subset of 26 patients from our RCT of diabetics with baseline serum 25(OH)D <25ng/mL randomized to vitamin D3 4000 IU/day or placebo for 4 months...
September 21, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28938443/microbiota-dependent-induction-of-colonic-cyp27b1-is-associated-with-colonic-inflammation-implication-of-locally-produced-1-25-dihydroxyvitamin-d-in-inflammatory-regulation-in-the-colon
#6
Jie Du, Xinzhi Wei, Xin Ge, Yinyin Chen, Yan Chun Li
Our recent studies demonstrated that intestinal epithelial vitamin D receptor (VDR) signaling plays a critical role in regulating colonic inflammation by protecting epithelial barrier integrity. Epithelial VDR is down-regulated in colitis, but how mucosal inflammation affects local 1,25-dihydroxyvitamin D (1,25(OH)2D3) production is unknown. Here we showed that Cyp27b1, a cytochrome P450 enzyme required for 1,25(OH)2D3 biosynthesis, is highly induced in colonic mucosa in inflammatory response. While VDR is reduced in colon biopsies from patients with ulcerative colitis, Cyp27b1 is markedly up-regulated in these samples...
September 18, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938396/transgenic-expression-of-the-vitamin-d-receptor-restricted-to-the-ileum-cecum-and-colon-of-vitamin-d-receptor-knockout-mice-rescues-vitamin-d-receptor-dependent-rickets
#7
Puneet Dhawan, Vaishali Veldurthy, Ghassan Yehia, Connie Hsaio, Angela Porta, Ki-In Kim, Nishant Patel, Liesbet Lieben, Lieve Verlinden, Geert Carmeliet, Sylvia Christakos
Although the intestine plays the major role in 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) action on calcium homeostasis, the mechanisms involved remain incompletely understood. The established model of 1,25(OH)2D3 regulated intestinal calcium absorption postulates a critical role for the duodenum. However it is the distal intestine where 70 -80% of the ingested calcium is absorbed. In order to test directly the role of 1,25(OH)2D3 and the vitamin D receptor (VDR) in the distal intestine, 3 independent knockout (KO) /transgenic (TG) lines expressing VDR exclusively in the ileum, cecum and colon were generated by breeding VDR KO mice with TG mice expressing human (h)VDR under the control of the 9...
September 11, 2017: Endocrinology
https://www.readbyqxmd.com/read/28926770/1-25-oh-2d3-induces-regulatory-t-cell-differentiation-by-influencing-the-vdr-plc-%C3%AE-1-tgf-%C3%AE-1-pathway
#8
Qiang Zhou, Shengying Qin, Jinyan Zhang, Lin Zhon, Zhihai Pen, Tonghai Xing
Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared to those with vitamin D deficiency. Thus, a high percentage of regulatory T cells might play a key role in preventing acute cellular rejection (ACR). In this report, we studied the specific effects of 1,25(OH)2D3 on human T cell diff ;erentiation, and determined the potential molecule mechanism behind...
September 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28925469/1-25-dihydroxyvitamin-d3-regulates-t-lymphocyte-proliferation-through-activation-of-p53-and-inhibition-of-erk1-2-signaling-pathway-in-children-with-kawasaki-disease
#9
X-L Qi, L-L Chen, X-G Sun, X-M Li, L-H Zhao, D-J Kong
OBJECTIVE: To study the effect of 1,25(OH)2D3 on T lymphocytes in Kawasaki disease and to further investigate its molecular mechanism. PATIENTS AND METHODS: A total of 30 child patients was diagnosed as Kawasaki disease, 60 child patients were diagnosed as infectious fever, and 60 normal children, were selected. 4 mL peripheral blood was collected before treatment. Peripheral blood mononuclear cells were isolated and separated using the Facollin method and cultured...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28922720/1-25-oh-2d3-deficiency-increases-tm40d-tumor-growth-in-bone-and-accelerates-tumor-induced-bone-destruction-in-a-breast-cancer-bone-metastasis-model
#10
Guangping Lu, Jun Li, Jiahui Chu, Shidai Jin, Ziyi Fu, Dengshun Miao, Yongmei Yin
Breast cancer is one of the most common malignancies and bone is the commonest site of distant metastases. Evidences indicate that adequate supply of vitamin D will decrease the morbidity and mortality of breast cancer. However, the main role of vitamin D deficiency in breast cancer bone metastases remains unclear. In this study, the relationship between vitamin D and breast cancer bone metastases were evaluated. Results showed that 1,25(OH)2D3 can not only inhibit the proliferation, migration and invasion of breast cancer cell TM40D in vitro, but also attenuate the breast cancer cell TM40D-induced bone destruction in vivo, whose underlying mechanism was at least partially through decreasing the number of the osteoclasts...
September 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28917261/development-of-standard-reference-material-srm-2973-vitamin-d-metabolites-in-frozen-human-serum-high-level
#11
Susan S-C Tai, Michael A Nelson, Mary Bedner, Brian E Lang, Karen W Phinney, Lane C Sander, James H Yen, Joseph M Betz, Christopher T Sempos, Stephen A Wise
The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health Office of Dietary Supplements and the Vitamin D Standardization Program, has recently issued a new serum-matrix Standard Reference Material (SRM): 2973 Vitamin D Metabolites in Frozen Human Serum (High Level). SRM 2973 was designed to provide a serum material with a total 25-hydroxyvitamin D [25(OH)D] concentration near 100 nmol/L to complement the existing serum-based SRMs with values assigned for total 25(OH)D between 20 and 80 nmol/L...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28904494/vitamin-d-deficiency-does-not-influence-reproductive-outcomes-of-ivf-icsi-a-study-of-oocyte-donors-and-recipients
#12
Manish Banker, Dipesh Sorathiya, Sandeep Shah
BACKGROUND: Vitamin D and its active metabolite, 1,25-dihydroxy vitamin D (1,25-(OH)2D3), play a significant role in reproduction. AIM: To assess the effect of serum 25-hydroxy vitamin D level on oocyte quality and endometrial receptivity by studying oocyte donors and their recipients. MATERIALS AND METHODS: This prospective study consisted of two groups: Group A (recipient group) and Group B (donor group). All the participants of Groups A1 and B1 as well as Groups A2 and B2 were subcategorized into vitamin D-deficient (<20 ng/mL) and vitamin D replete-insufficient (20 to ≥30 ng/mL), respectively...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28901396/effect-of-1-25-oh-2d3-on-high-glucose%C3%A2-induced-autophagy-inhibition-in-peritoneum
#13
Lina Yang, Yi Fan, Xiuli Zhang, Jin Liu, Jianfei Ma
High glucose (HG) may damage the structure and function of the peritoneal membrane, and is considered to be one of the most important factors that leads to peritoneal fibrosis and ultrafiltration failure. Recently, 1,25(OH)2D3, the active form of vitamin D, was demonstrated to protect against epithelial‑mesenchymal transition and fibrosis in peritoneal mesothelium and other organs. Accumulating evidence has suggested that autophagy serves a protective role in certain diseases by regulating cell survival...
August 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28893622/differential-expression-of-vitamin-d-associated-enzymes-and-receptors-in-brain-cell-subtypes
#14
Véréna Landel, Delphine Stephan, Xiaoying Cui, Darryl Eyles, François Feron
Accumulating evidence indicates that the active form of vitamin D, 1,25(OH)2D3, can be considered as a neurosteroid. However, the cerebral expression of vitamin D-associated enzymes and receptors remains controversial. With the idea of carrying out a comparative study in mind, we compared the transcript expression of Cyp27a1, Cyp27b1, Cyp24a1, Vdr and Pdia3 in purified cultures of astrocytes, endothelial cells, microglia, neurons and oligodendrocytes. We observed that endothelial cells and neurons can possibly transform the inactive cholecalciferol into 25(OH)D3...
September 8, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28870774/ets-transcription-factor-family-member-gabpa-contributes-to-vitamin-d-receptor-target-gene-regulation
#15
Sabine Seuter, Antonio Neme, Carsten Carlberg
Binding motifs of the ETS-domain transcription factor GABPA are found with high significance below the summits of the vitamin D receptor (VDR) cistrome. VDR is the nuclear receptor for the biologically most active vitamin D metabolite 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3). In this study, we determined the GABPA cistrome in THP-1 human monocytes and found that it is comprised of 3822 genomic loci, some 20% of which were modulated by 1,25(OH)2D3. The GABPA cistrome showed a high overlap rate with accessible chromatin and the pioneer transcription factor PU...
September 11, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28864231/improved-sensitivity-of-serum-plasma-1%C3%AE-25-dihydroxyvitamin-d-quantification-by-daptad-derivatization
#16
Takayuki Ishige, Mamoru Satoh, Shoujiro Ogawa, Motoi Nishimura, Kazuyuki Matsushita, Tatsuya Higashi, Fumio Nomura
BACKGROUND: Although immunoassays have several limitations such as the cross-reactivities of antibodies, such techniques are widely used for serum/plasma 1,25(OH)2D quantification. An accurate method is required for the determination of the 1,25(OH)2D status. METHODS: We designed a serum/plasma 1,25(OH)2D quantification method using LC-MS/MS. Immunoaffinity extraction (IE) and the recently developed Cookson-type reagent 4-(4'-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) were used for sample preparation and derivatization, respectively...
October 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28860545/1%C3%AE-20s-dihydroxyvitamin-d3-interacts-with-vitamin-d-receptor-crystal-structure-and-route-of-chemical-synthesis
#17
Zongtao Lin, Hao Chen, Anna Y Belorusova, John C Bollinger, Edith K Y Tang, Zorica Janjetovic, Tae-Kang Kim, Zhongzhi Wu, Duane D Miller, Andrzej T Slominski, Arnold E Postlethwaite, Robert C Tuckey, Natacha Rochel, Wei Li
1α,20S-Dihydroxyvitamin D3 [1,20S(OH)2D3], a natural and bioactive vitamin D3 metabolite, was chemically synthesized for the first time. X-ray crystallography analysis of intermediate 15 confirmed its 1α-OH configuration. 1,20S(OH)2D3 interacts with the vitamin D receptor (VDR), with similar potency to its native ligand, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] as illustrated by its ability to stimulate translocation of the VDR to the nucleus, stimulate VDRE-reporter activity, regulate VDR downstream genes (VDR, CYP24A1, TRPV6 and CYP27B1), and inhibit the production of inflammatory markers (IFNγ and IL1β)...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28859991/1%C3%AE-25-dihydroxyvitamin-d3-promotes-osteogenesis-by-promoting-wnt-signaling-pathway
#18
Yi Xiong, Yixin Zhang, Na Xin, Ying Yuan, Qin Zhang, Ping Gong, Yingying Wu
Diabetes mellitus (DM) remarkably affects bone metabolism and causes multiple skeletal disorders, which are associated with the increased oxidative stress that activates Forkhead family of transcription factors (FoxOs). 1α,25-Dihydroxy vitamin D3 (1,25(OH)2D3), the hormonally active form of vitamin D, plays a potential role in the prevention of glucose tolerance. However, its mechanism of action in high glucose-induced energy disorders remains unclear. In vitro study shows that 1,25(OH)2D3 promotes osteogenesis in high glucose-induced oxidative stress mainly results from increased osteoblasts proliferation and decreased apoptosis...
August 30, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28842174/a-fast-and-simple-method-for-simultaneous-measurements-of-25-oh-d-24-25-oh-2d-and-the-vitamin-d-metabolite-ratio-vmr-in-serum-samples-by-lc-ms-ms
#19
Neus Fabregat-Cabello, Jordi Farre-Segura, Loreen Huyghebaert, Stéphanie Peeters, Caroline Le Goff, Jean-Claude Souberbielle, Étienne Cavalier
BACKGROUND: Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. METHODS: Sample preparation was based on precipitation and centrifugation of 100μL of patient serum, followed by injection into the LC-MS/MS system...
October 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28821819/dietary-factors-and-polymorphisms-in-vitamin-d-metabolism-genes-the-risk-and-prognosis-of-colorectal-cancer-in-northeast-china
#20
Chen Gong, Zhiping Long, Yanming Yu, Lin Zhu, Jingshen Tian, Shuo Li, Jing Li, Hongyuan Yu, Qiang Chi, Daxun Piao, Fan Wang, Yashuang Zhao, Binbin Cui
CYP24A1 and CYP27B1 are critical genes determining 1α,25(OH)2D3 concentration and impacting on carcinogenesis. A case-control study including 528 colorectal cancer (CRC) patients and 605 cancer-free controls and a follow-up study with 317 cases were conducted in northeast China. Genotypes were tested by TaqMan Genotyping Assays. Individuals carrying the GG genotype of CYP27B1 G > T (rs10877012) exhibited decreased CRC risk compared with those with the TT genotype (ORadjusted (ORadj) = 0.57, 95% Confidence Interval (CI) = 0...
August 18, 2017: Scientific Reports
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