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1,25(OH)2D3

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https://www.readbyqxmd.com/read/28716760/properties-of-purified-cyp2r1-in-a-reconstituted-membrane-environment-and-its-25-hydroxylation-of-20-hydroxyvitamin-d3
#1
Chloe Y S Cheng, Tae-Kang Kim, Saowanee Jeayeng, Andrzej T Slominski, Robert C Tuckey
Recent studies indicate that CYP2R1 is the major 25-hydroxylase catalyzing the first step in vitamin D activation. Since the catalytic properties of CYP2R1 have been poorly studied to date and it is a membrane protein, we examined the purified enzyme in a membrane environment. CYP2R1 was expressed in E. coli and purified by nickel affinity- and hydrophobic interaction-chromatography and assayed in a reconstituted membrane system comprising phospholipid vesicles plus purified human NADPH-P450 oxidoreductase...
July 14, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28712921/selective-regulation-of-biological-processes-by-vitamin-d-based-on-the-spatio-temporal-cistrome-of-its-receptor
#2
Antonio Neme, Sabine Seuter, Carsten Carlberg
The transcription factor vitamin D receptor (VDR) is the exclusive nuclear target of the biologically active form of vitamin D (1,25(OH)2D3). In THP-1 human monocytes we obtained a highly accurate VDR cistrome after 2 and 24 h ligand stimulation comprising more than 11,600 genomic loci, 78% of which were detected exclusively after 24 h. In contrast, a group of 510 persistent VDR sites occurred at all conditions and some 2,100 VDR loci were only transiently occupied. Machine learning and statistical analysis as well as a comparison with the re-analyzed B cell VDR cistrome indicated a subgroup of 339 highly conserved persistent VDR sites that were suited best for describing vitamin D-triggered gene regulatory scenarios...
July 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28710602/eldecalcitol-ed-71-an-analog-of-1%C3%AE-25-oh-2d3-inhibits-the-growth-of-squamous-cell-carcinoma-scc-cells-in-vitro-and-in-vivo-by-down-regulating-expression-of-heparin-binding-protein-17-fibroblast-growth-factor-binding-protein-1-hbp17-fgfbp-1-and-fgf-2
#3
T Shintani, F Takatsu, S N Z Rosli, E Usui, A Hamada, K Sumi, Y Hayashido, S Toratani, Tetsuji Okamoto
Heparin-binding protein 17 (HBp17)/fibroblast growth factor-binding protein-1 (FGFBP-1) was first purified from medium conditioned by A431 cells for its capacity to bind to fibroblast growth factors 1 and 2 (FGF-1 and -2). Among FGF family members, FGF-2 is a potent mitogen for various cell types, including vascular endothelial cells, fibroblasts, and cancer cells such as oral squamous cell carcinoma (OSCC) cells. Besides being well known in bone metabolism, the active form of vitamin D3, i.e., 1α,25(OH)2D3 (1,25D3), was reported to have protective effects for heart disease and cancer...
July 14, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28694529/the-production-of-fibroblast-growth-factor-23-is-controlled-by-tgf-%C3%AE-2
#4
Martina Feger, Philipp Hase, Bingbing Zhang, Frank Hirche, Philipp Glosse, Florian Lang, Michael Föller
Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in cell growth, differentiation, apoptosis, and inflammation. It stimulates store-operated calcium entry (SOCE) through the calcium release-activated calcium (CRAC) channel Orai1/Stim1 in endometrial Ishikawa cells. Bone cells generate fibroblast growth factor (FGF) 23, which inhibits renal phosphate reabsorption and 1,25(OH)2D3 formation in concert with its co-receptor Klotho. Moreover, Klotho and FGF23 counteract aging and age-related clinical conditions...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676457/on-the-role-of-classical-and-novel-forms-of-vitamin-d-in-melanoma-progression-and-management
#5
REVIEW
Andrzej T Slominski, Anna A Brozyna, Cezary Skobowiat, Michal A Zmijewski, Tae-Kang Kim, Zorica Janjetovic, Allen S Oak, Wojciech Jozwicki, Anton M Jetten, Rebecca S Mason, Craig Elmets, We Li, Robert M Hoffman, Robert C Tuckey
Melanoma represents a significant clinical problem affecting a large segment of the population with a relatively high incidence and mortality rate. Ultraviolet radiation (UVR) is an important etiological factor in malignant transformation of melanocytes and melanoma development. UVB, while being a full carcinogen in melanomagenesis, is also necessary for the cutaneous production of vitamin D3 (D3). Calcitriol (1,25(OH)2D3) and novel CYP11A1-derived hydroxyderivatives of D3 show anti-melanoma activities and protective properties against damage induced by UVB...
July 1, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28669880/role-of-vitamin-d-in-amyloid-clearance-via-lrp-1-upregulation-in-alzheimer-s-disease-a-potential-therapeutic-target
#6
REVIEW
Parmi Patel, Jigna Shah
Amyloid beta (Aβ) deposition is considered to be one of the primary reason to trigger Alzheimer's disease (AD). Literature clearly suggests decline in Aβ clearance to be accountable for progression of late onset AD as compared to augmented Aβ production. There may be several pathways for Aβ clearance out of which one of the major pathway is the vascular-mediated removal of Aβ from the brain across the blood-brain barrier (BBB) via efflux pumps or receptors. Among Aβ scavenger receptors, low density lipoprotein receptor related protein (LRP-1) has been most extensively studied...
June 30, 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/28665452/genetic-polymorphisms-of-vitamin-d-metabolism-genes-and-serum-level-of-vitamin-d-in-colorectal-cancer
#7
Verônica Marques Vidigal, Pedro Nazareth Aguiar Junior, Tiago Donizetti Silva, Juliana de Oliveira, Célia Aparecida Marques Pimenta, Aledson Vitor Felipe, Nora Manoukian Forones
BACKGROUND: The metabolism of vitamin D is complex, its receptor (VDR) and proteins encoded by the genes CYP27B2 and CYP24A1 can influence vitamin D serum levels. The aim of this study was to investigate the relationship of the polymorphisms of VDR (ApaI and BsmI), CYP27B1 and CYP24A1 with serum vitamin D levels in both forms, 25(OH)D3 (circulating form) and 1,25(OH)2D3 (active form), in colorectal cancer (CRC) patients. METHODS: One hundred fifty-two CRC patients and 321 controls were included...
June 19, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/28651973/1-25-oh-2d3-disrupts-glucose-metabolism-in-prostate-cancer-cells-leading-to-a-truncation-of-the-tca-cycle-and-inhibition-of-txnip-expression
#8
Mohamed A Abu El Maaty, Hamed Alborzinia, Shehryar J Khan, Michael Büttner, Stefan Wölfl
Prostate cell metabolism exhibits distinct profiles pre- and post-malignancy. The malignant metabolic shift converts prostate cells from "citrate-producing" to "citrate-oxidizing" cells, thereby enhancing glucose metabolism, a phenotype that contrasts classical tumoral Warburg metabolism. An on-line biosensor chip system (BIONAS 2500) was used to monitor metabolic changes (glycolysis and respiration) in response to the putative anti-cancer nutraceutical 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], in different prostate cancer (PCa) cell lines (LNCaP, VCaP, DU145 and PC3)...
June 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28624282/effect-of-endoplasmic-reticulum-stress-on-metabolic-and-stress-signaling-and-kidney-specific-functions-in-madin-darby-bovine-kidney-cells
#9
E Chiappisi, R Ringseis, K Eder, D K Gessner
Recent studies demonstrated induction of endoplasmic reticulum (ER) stress in tissues of cows after parturition, but knowledge about the effect of ER stress on important cellular processes, such as critical signaling and metabolic pathways, in cattle is scarce. Thus, the present study aimed to investigate the effect of ER stress induction on nuclear factor-κB (NF-κB), nuclear factor E2-related factor 2 (Nrf2), and sterol regulatory element-binding protein (SREBF1) pathway in Madin-Darby bovine kidney (MDBK) cells, a widely used in vitro model in ruminant research...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28602960/the-impact-of-vdr-expression-and-regulation-in-vivo
#10
Seong Min Lee, Mark B Meyer, Nancy A Benkusky, Charles A O'Brien, J Wesley Pike
The vitamin D receptor (VDR) mediates the pleiotropic biological actions of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). These actions include orchestration of mineral homeostasis which is coordinated by the kidney, intestine, bone and parathyroid gland wherein the VDR transcriptionally regulates expression of the genes involved in this complex process. Mutations in human VDR (hVDR) cause hereditary vitamin D resistant rickets, a genetic syndrome characterized by hypocalcemia, hyperparathyroidism and rickets resulting from dysregulation of mineral homeostasis...
June 9, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28591703/vitamin-d-receptor-activation-reduces-vcap-xenograft-tumor-growth-and-counteracts-erg-activity-despite-induction-of-tmprss2-erg
#11
Justin M Roberts, Rebeca San Martin, D Badrajee Piyarathna, James G MacKrell, Guilherme V Rocha, Jeffery A Dodge, Cristian Coarfa, Venkatesh Krishnan, David R Rowley, Nancy L Weigel
Whether vitamin D is chemopreventive and/or has potential therapeutically in prostate cancer is unresolved. One confounding factor is that many prostate cancers express a TMPRSS2:ERG fusion gene whose expression is increased both by androgens and by vitamin D receptor (VDR) activation. Two challenges that limit VDR agonist use clinically are hypercalcemia and the cooperation of VDR with ERG to hyper-induce the 1α,25-dihydroxyvitamin D3 metabolizing enzyme, CYP24A1, thus reducing VDR activity. Using the VCaP TMPRSS2:ERG positive cell line as a model, we found that a nonsecosteroidal CYP24A1 resistant VDR agonist, VDRM2, substantially reduces growth of xenograft tumors without inducing hypercalcemia...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28587998/clinical-and-genetic-analysis-of-two-chinese-families-with-vitamin-d-dependent-rickets-type-ia-and-follow-up
#12
Chen Ruimin, Li Yunfei, Yuan Xin, Lin Xiangquan, Yang Xiaohong, Zhang Ying
OBJECTIVE: Vitamin D-dependent rickets type IA (VDDR-IA) is a rare autosomal recessive disorder characterized by the early onset of severe rickets. The objectives of this study were twofold: 1) to analyze the clinical characteristics and therapy of two patients with VDDR-IA from two separate Chinese family, and 2) investigate the CYP27B1 gene in two large pedigrees. METHODS: Medical history, clinical manifestations, physical examination, radiological findings and laboratory data were analyzed from two patients with VDDR-IA...
June 7, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/28579120/vitamin-d-and-the-brain-genomic-and-non-genomic-actions
#13
REVIEW
Xiaoying Cui, Helen Gooch, Alice Petty, John J McGrath, Darryl Eyles
1,25(OH)2D3 (vitamin D) is well-recognized as a neurosteroid that modulates multiple brain functions. A growing body of evidence indicates that vitamin D plays a pivotal role in brain development, neurotransmission, neuroprotection and immunomodulation. However, the precise molecular mechanisms by which vitamin D exerts these functions in the brain are still unclear. Vitamin D signalling occurs via the vitamin D receptor (VDR), a zinc-finger protein in the nuclear receptor superfamily. Like other nuclear steroids, vitamin D has both genomic and non-genomic actions...
June 1, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28578001/1%C3%AE-25-dihydroxyvitamin-d3-enhances-trpv6-transcription-through-p38-mapk-activation-and-gadd45-expression
#14
Michiyasu Ishizawa, Daisuke Akagi, Jumpei Yamamoto, Makoto Makishima
The active form of vitamin D, 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3], acts as a ligand for the vitamin D receptor (VDR), and regulates various physiological processes, including calcium and bone metabolism, cellular growth and differentiation, immunity and cardiovascular function. A number of vitamin D derivatives have been synthesized for the treatment of cancer and inflammatory disease, but the adverse effect of hypercalcemic activity due to intestinal calcium absorption has limited wide clinical application...
May 31, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28565795/1-25-oh-2d3-and-its-analogue-bxl-628-inhibit-high-glucose-induced-activation-of-rhoa-rock-pathway-in-hk-2-cells
#15
Wei Zhang, Bin Yi, Ke Zhang, Aimei Li, Shikun Yang, Jing Huang, Jishi Liu, Hao Zhang
It has previously been reported that 1,25-(OH)2D3 inhibits high glucose-induced epithelial-to-mesenchymal transition (EMT) in HK-2 cells. However, the mechanism of this renoprotective action remains unclear. Elocalcitol (BXL-628), a vitamin D analog, has been suggested to be effective on the RhoA/Rho associated protein kinase (ROCK) pathway, which serves a crucial role in high glucose-induced EMT. The aim of the present study was to investigate the effect of 1,25-(OH)2D3 and its analogue BXL-628 on high glucose-induced activation of the RhoA/ROCK pathway in human renal proximal tubular cells...
May 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28552332/1-25-dihydroxyvitamin-d3-suppressed-experimental-autoimmune-encephalomyelitis-through-both-immunomodulation-and-oligodendrocyte-maturation
#16
Hasti Atashi Shirazi, Javad Rasouli, Bogoljub Ciric, Danmeng Wei, Abdolmohamad Rostami, Guang-Xian Zhang
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) has recently been found to have the anti-inflammatory potential to suppress experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis; however, its direct effect on neural cells is not clear. In the current study we show that 1,25(OH)2D3 treatment effectively suppressed clinical signs of ongoing EAE and reduced inflammation and demyelination scores in the central nervous system (CNS). The treatment significantly decreased production/expression of pro-inflammatory cytokines IFN-γ, GM-CSF and IL-17A, while it increased anti-inflammatory cytokines IL-4 and IL-10...
May 25, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28549199/effect-of-1-25-oh2d3-on-the-proliferation-of-human-mesangial-cells-and-their-expression-of-ki67
#17
D Zhao, C-J Zhang, R Yang, J-P Chen, L Ma, G Liu, X-P Yang
Previous studies have found that 1,25-dihydroxyvitamin D3 [1,25(OH)2D3 or VD3] exerts many biological effects, including the inhibition of cell proliferation and induction of apoptosis, but its mechanism of action remains unclear. The goal of our investigation was to explore the effects of 1,25(OH)2D3 on the proliferation of cultured human mesangial cells and their expression of Ki67 in vitro, and to establish its mechanism of action. Cultured human mesangial cells were randomly divided into the following four groups: normal control (N group; administered Dulbecco's modified Eagle's medium containing 5% fetal bovine serum), proliferation [epidermal growth factor (EGF) group; administered 10 μg/L EGF], VD3 intervention [administered 10(-8) M 1,25(OH)2D3], and proliferation and intervention [EGF+VD3 group; administered 10 μg/L EGF and 10(-8) M 1,25(OH)2D3]...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28540351/cytokine-data-obtained-from-synovial-stromal-cells-of-patients-with-rheumatoid-arthritis-or-osteoarthritis
#18
Johanna A Huhtakangas, Johanna Veijola, Sanna Turunen, Anna Karjalainen, Maarit Valkealahti, Tomi Nousiainen, Susanna Yli-Luukko, Olli Vuolteenaho, Petri Lehenkari
In this article, we share the raw cytokine data obtained from basal and stimulated synovial stromal cells cultured from patients with rheumatoid arthritis or osteoarthritis. This data article is related to the research article entitled "1,25D3 and calcipotriol, its hypocalcemic analog, exert a long-lasting anti-inflammatory and anti-proliferative effect in synoviocytes cultured from patients with rheumatoid arthritis and osteoarthritis (1). Cytokine levels were analyzed by a magnetic bead-based multiplex assay (a panel of 27 important cytokines) in two separate sets of experiments...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28539530/active-form-of-vitamin-d-ameliorates-non-alcoholic-fatty-liver-disease-by-alleviating-oxidative-stress-in-a-high-fat-diet-rat-model
#19
Chong-Gui Zhu, Ya-Xin Liu, Hao Wang, Bao-Ping Wang, Hui-Qi Qu, Bao-Li Wang, Mei Zhu
The purpose of this study was to determine whether treatment using the active form of vitamin D (1,25(OH)2D3) could protect against high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in rats and ameliorate oxidative stress. Male Sprague-Dawley rats were divided into three groups and treated with standard chow, HFD, or HFD plus intraperitoneal injection of 1,25(OH)2D3 (5 μg/kg body weight, twice per week), respectively, for 16 weeks. Serum lipid profiles, hepatic function, intrahepatic lipid, and calcium levels were determined...
May 23, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28536085/interaction-between-the-effects-of-the-selective-estrogen-modulator-femarelle-and-a-vitamin-d-analog-in-human-umbilical-artery-vascular-smooth-muscle-cells
#20
Dalia Somjen, Esther Knoll, Orli Sharon, Ariel Many, Naftali Stern
To further investigate the interaction between vitamin D system and estrogen-mimetic compounds in the human vasculature we studied the effect of the "less- calcemic" analog of 1,25(OH)2D3 (1,25D); JK 1624F2-2 (JKF) in the presence of selective estrogen modulator femarelle (F), the phytoestrogen daidzein (D) and estradiol-17b (E2) on (3)[H] thymidine incorporation (DNA synthesis) and creatine kinase specific activity (CK) in human umbilical artery vascular smooth muscle cells (VSMC). F, D and E2, stimulated DNA synthesis at low concentrations, and inhibited it at high concentrations...
May 20, 2017: Journal of Steroid Biochemistry and Molecular Biology
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