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Bioimpedance assay

Elena Dozio, Silvia Briganti, Alessandra Delnevo, Elena Vianello, Federica Ermetici, Francesco Secchi, Francesco Sardanelli, Lelio Morricone, Alexis E Malavazos, Massimiliano M Corsi Romanelli
PURPOSE: Soluble receptor for advanced glycation end products (sRAGE) is a decoy receptor which sequesters RAGE ligands and acts as a cytoprotective agent. To date, it is unclear whether the lower sRAGE levels observed in obesity are a marker of increased overall adiposity or reflect increases in particular fat depots. Therefore, we evaluated in healthy women the relationship among sRAGE and indicators of adiposity, including abdominal visceral (VAT) and epicardial visceral (EAT) adipose tissues, to explore the potential role of sRAGE as an earlier biomarker of cardiometabolic risk...
August 13, 2016: European Journal of Nutrition
Bogna Grygiel-Górniak, Elżbieta Kaczmarek, Maria Mosor, Juliusz Przysławski, Anna Bogacz
The relationship between the genetic background, adipocytokines, and metabolic state in postmenopausal women has not yet been fully described. The aim of this study was to determine the relationship between PPAR gamma-2 (Pro12Ala, C1431T) and ADRB3 (Trp64Arg) polymorphisms and serum adipocytokines (adiponectin, visfatin, and resistin) and metabolic disorders in 176 postmenopausal women with increased body mass (BMI ≥ 25 kg m(-2)). The distributions of selected alleles and genotype frequencies were determined with the PCR-RFLP method...
October 2016: Biochemical Genetics
Jayasurya Ravindranath, Priyamvada P Sivan Pillai, Sreejith Parameswaran, Sadish Kumar Kamalanathan, Gopal Krushna Pal
OBJECTIVES: Body composition analysis is required for accurate assessment of nutritional status in patients with predialysis chronic kidney disease (CKD). The reference method for assessing body fat is dual-energy X-ray absorptiometry (DXA), but it is relatively expensive and often not available for widespread clinical use. There is only limited data on the utility of less expensive and easily available alternatives such as multifrequency bioimpedance assay (BIA) and skinfold thickness (SFT) measurements for assessing body fat in predialysis CKD...
September 2016: Journal of Renal Nutrition
Kamal Hassan, Batya Kristal, Fadi Hassan, Saad Abo Saleh, Regina Michelis
OBJECTIVE: Hypoalbuminemia, fluid overload (FO), and oxidative stress (OS) may be related to cardiovascular morbidity and mortality in peritoneal dialysis (PD) patients. OS produces molecular modifications of serum albumin that interfere with its quantification by the commonly used bromocresol green assay. This study evaluated the impact of oxidized serum albumin (OSA) on oncotic pressure (OP) and hydration status. PATIENTS AND METHODS: Twenty-four stable hypoalbuminemic PD patients were enrolled in the study...
2016: Therapeutics and Clinical Risk Management
Marco Tatullo, Massimo Marrelli, Massimiliano Amantea, Francesco Paduano, Luigi Santacroce, Stefano Gentile, Salvatore Scacco
INTRODUCTION: Bioimpedance is a measure of the electrical properties of biological tissues. In the last two decades bioimpedance has been successfully introduced in clinical diagnosis of cancer. It has been demonstrated that tumoral tissues often show lower bioimpedance values than healthy tissues. The aim of this work is to assess the bioimpedentiometric differences between healthy and Oral Lichen Planus (OLP) affected oral mucosa, taking attention to the erosive form which may represent a potential pre-cancerous condition...
2015: Journal of Cancer
Bogna Grygiel-Górniak, Elżbieta Kaczmarek, Maria Mosor, Juliusz Przysławski, Jerzy Nowak
The aim of this study was to test the association of peroxisome proliferator-activated receptor (PPAR-γ2) (Pro12Ala, C1431T) and β3-AR (Trp64Arg) polymorphisms with metabolic, nutritional, and blood pressure parameters in 271 postmenopausal women (151 hypertensive and 120 normotensive controls). The TaqMan genotyping assay and restriction fragment length polymorphism methods were used to determine the distributions of selected alleles and genotype frequencies. Nutritional status was determined by a bioimpedance method and dietary habits were assessed via 7-day dietary recall...
July 2015: Journal of Clinical Hypertension
Eren Gurkan, Ilhan Tarkun, Tayfun Sahin, Berrin Cetinarslan, Zeynep Canturk
AIMS: To demonstrate the efficacy of exenatide versus insulin glargine on endothelial functions and cardiovascular risk markers. METHODS: Thirty-four insulin and incretin-naive patients with type 2 diabetes mellitus (body mass index 25-45 kg/m(2)) who received metformin for at least two months were randomized to exenatide or insulin glargine treatment arms and followed-up for 26 weeks. Measurements of endothelial functions were done by ultrasonography, cardiovascular risk markers by serum enzyme-linked immunosorbent assay, and total body fat mass by bioimpedance...
December 2014: Diabetes Research and Clinical Practice
S Małgorzewicz, A Dębska-Slizień, B Czajka, B Rutkowski
BACKGROUND: Obesity and disturbances of adipokine concentrations are often recognized in kidney transplant recipients (KTRs). Leptin plays a key role in regulating energy intake and expenditure, including appetite and hunger, metabolism, and behavior. Adiponectin modulates certain metabolic processes, including glucose regulation and fatty acid oxidation, and exerts some weight-reduction effects. Visfatin has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis...
October 2014: Transplantation Proceedings
Nilsel Okudan, Hakkı Gökbel, Hasan Acar, Selim Uzunoğlu, Muaz Belviranli
The aim of the present study was to evaluate the relationship between oligopolymorphism in the 25th codon of leptin gene and obesity. Eighty-seven obese women and 75 healthy women were constituted obese and control groups. Body fat percent, fat mass and lean body mass were determined by bioimpedance meter and leptin levels were determined. The presence of 25th codon oligopolymorphism in the leptin gene was done by PCR-RFLP technique. Mean leptin levels were 38.5±22.0 ng/ml, and 147.9±44.8 ng/ml in the control and obese groups, respectively...
October 2014: Archives of Physiology and Biochemistry
Renata Rubinsztajn, Tadeusz Przybyłowski, Marta Maskey-Warzęchowska, Krzysztof Karwat, Magdalena Paplińska-Goryca, Patrycja Nejman-Gryz, Ryszarda Chazan
UNLABELLED: INTRODUCTION Exacerbations affect the natural history of chronic obstructive pulmonary disease (COPD). OBJECTIVES: The aim of the study was to evaluate the effect of exacerbation frequency in COPD on body composition and systemic inflammation assessed by the measurement of serum adiponectin and ghrelin concentrations. PATIENTS AND METHODS: The study group included 152 patients with COPD. Body composition was assessed by bioimpedance...
2014: Polskie Archiwum Medycyny Wewnętrznej
Winnie Chan, Jos A Bosch, David Jones, Philip G McTernan, Nicholas Inston, Sue Moore, Okdeep Kaur, Anna C Phillips, Richard Borrows
BACKGROUND: The prevalence and consequences of hypervolemia in kidney transplant recipients (KTRs) have not been investigated. Specifically, its impact on blood pressure (BP) and relationship with N-terminal fragment of prohormone B-type natriuretic peptide (NT-proBNP) are unknown. The objectives of this study were to establish the prevalence of hypervolemia among clinically stable KTRs, investigate the predictors of posttransplant hypervolemia, assess its impact on blood pressure, and determine its relationship with NT-proBNP...
August 15, 2014: Transplantation
Hong Xu, Ivan Cabezas-Rodriguez, Abdul Rashid Qureshi, Olof Heimburger, Peter Barany, Sunna Snaedal, Björn Anderstam, Ann-Christin Bragfors Helin, Juan Jesus Carrero, Peter Stenvinkel, Bengt Lindholm
UNLABELLED:BACKGROUND AND AIMS: Oxidative stress plays an important role in the pathogenesis of cardiovascular disease (CVD). Central blood pressure (BP) is thought to be more relevant than peripheral BP for the pathogenesis of CVD. Advanced oxidation protein products (AOPP) are markers of oxidative stress. This study investigated the relationship between AOPP and central BP in peritoneal dialysis (PD) patients. ♦ METHODS: In a cross-sectional study of 75 PD patients (67% men), we analyzed two oxidative stress markers, AOPP (modified assay, mAOPP, correcting for the impact of triglycerides) and pentosidine, three inflammation markers, interleukin-6 (IL-6), tumor necrosis factor (TNF), and high-sensitivity C-reactive protein (hs-CRP)...
July 2015: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
Fátima López-Alcaraz, Mario Del Toro-Equihua, Mariana Orta-Duarte, Yunue Flores-Ruelas, Carmen Alicia Sánchez-Ramírez
INTRODUCTION: The development of chronic-degenerative diseases secondary to obesity in early infancy has alerted health providers to the importance of identifying the risk factors for obesity and assessing preventive treatment to reduce risks. Studies performed on a pediatric population have examined the role of inflammatory biomarkers (specifically CRP and TNF-α) and adiposity with inconsistent results. OBJECTIVE: To assess the relationship between the serum levels of C-reactive protein and tumor necrosis factor-alfa with adiposity measured by bioimpedance analysis in schoolchildren...
2014: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Devasier Bennet, Se Chan Kang, Jongback Gang, Sanghyo Kim
Plants contain enriched bioactive molecules that can protect against skin diseases. Bioactive molecules become unstable and ineffective due to unfavorable conditions. In the present study, to improve the therapeutic efficacy of phytodrugs and enhance photoprotective capability, we used poly(D,L-lactide-co-glycolide) as a carrier of apple peel ethanolic extract (APETE) on permeation-enhanced nanoparticles (nano-APETE). The in vitro toxicity of nano-APETE-treated dermal fibroblast cells were studied in a bioimpedance system, and the results coincided with the viability assay...
2014: International Journal of Nanomedicine
Ana B Crujeiras, María Pardo, Roca-Rivada Arturo, Santiago Navas-Carretero, M Angeles Zulet, J Alfredo Martínez, Felipe F Casanueva
OBJECTIVE: The recently discovered peptide irisin has been hypothesized to be a regulator of body metabolism. The objective of this work was to evaluate whether circulating human irisin levels are modulated by body size and changes in adiposity during an energy restriction treatment and the subsequent weight regain. METHODS: A group of 94 obese patients (50 men, 44 women; 49.4 ± 9.4 years; BMI 35.6 ± 4.5 kg/m(2) ) participated in a weight loss program following an 8-week hypocaloric diet (-30% energy expenditure) with a weight maintenance follow-up...
March 2014: American Journal of Human Biology: the Official Journal of the Human Biology Council
Ana Paula Pereira Castro, Ana Paula Carlos Cândido, Roney Luiz de Carvalho Nicolato, Ivo Santana Caldas, George Luiz Lins Machado-Coelho
PURPOSE: Evidence suggests that plasma retinol-binding protein 4 (RBP4) and insulin resistance are related to body fat (BF). We aimed to assess the relationship between RBP4 and insulin resistance with obesity in a mixed (skin color) cohort of the Brazilian population. METHODS: A nested case-control study was conducted in 227 schoolchildren aged 7-14 years. Schoolchildren with a high BF percentage (% BF, ≥ 30 for girls and ≥ 25 for boys) were identified as the obese group (n = 137), and those with lower values were identified as the non-obese group (n = 90)...
2014: European Journal of Nutrition
Mihály Tapolyai, Mária Faludi, Virág Réti, Zsolt Lengvárszky, Tibor Szarvas, Tibor Fülöp, Gabriella Bekő, Klára Berta
Correct estimation of the dialysis patients' hydration status remains an important clinical challenge. Bioimpedance measurements have been validated by various physiological tests, and the use of brain-type natriuretic peptide (BNP) has been validated by inferior vena cava diameter measurements. This is an observational cohort study that evaluated the correspondence between bioimpedance-measured overhydration percentage (OH%) and BNP. We measured predialysis OH% by bioimpedance apparatus (Body Composition Monitor) and BNP by microparticle enzyme-linked immunoassay in 41 prevalent stable hemodialysis patients, 19 (46%) women, aged 58...
July 2013: Hemodialysis International
Andrés Duarte-Rojo, Miguel Angel Torres-Vega, Hugo Villamil-Ramírez, José Estradas, Aarón Domínguez-López, Fausto Sánchez-Muñoz, Arturo Orea-Tejeda, Lilia Castillo-Martínez, Angel Miliar-García, Ricardo Ulises Macías-Rodríguez, Aldo Torre
BACKGROUND: Glutamine synthetase (GS) plays a central role in the inter-organ metabolism of ammonia and hepatic encephalopathy. The main objective of the present work was to disclose the possible effect of exercise on GS mRNA expression in peripheral blood mononuclear cells (PBMC) within a group of healthy volunteers. MATERIAL AND METHODS: PBMC were studied instead of skeletal muscle because of ethical concerns. Characterization of GS in lymphocytes was carried out by indirect immunofluorescence and Western blot...
March 2012: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
Antti Huuskonen, Jani Lappalainen, Niku Oksala, Matti Santtila, Keijo Häkkinen, Heikki Kyröläinen, Mustafa Atalay
PURPOSE: We clarified the effect of insulin-like growth factor-1 (IGF1), IGF-binding protein-3 (IGFBP3), interleukin-6 (IL6), and its receptor (IL6R) gene variants on muscular and aerobic performance, body composition, and on circulating levels of IGF-1 and IL-6. Single nucleotide polymorphisms (SNPs) may, in general, influence gene regulation or its expression, or the structure and function of the corresponding protein, and modify its biological effects. IGF-1 is involved in the anabolic pathways of skeletal muscle...
December 2011: Medicine and Science in Sports and Exercise
Karthikeyan Kandasamy, Cheol Soo Choi, Sanghyo Kim
In the emerging nanotechnology field, there is an urgent need for the development of a significant and sensitive method that can be used to analyse and compare the cytotoxicities of nanomaterials such as carbon nanotubes (CNTs) and gold nanoparticles (AuNPs), since such materials can be applied as contrast agents or drug delivery carriers. The bioimpedance system possesses great potential in many medical research fields including nanotechnology. Electric cell-substrate impedance sensing (ECIS) is a particular bioimpedance system that offers a real-time, non-invasive, and quantitative measurement method for the cytotoxicity of various materials...
September 17, 2010: Nanotechnology
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