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dengue pakistan

Mamoona Chaudhry, Saeed Ahmad, Hamad Bin Rashid, Iftikhar Ud Din
Swat, a lush green valley of 1.3 million persons, remained under militant insurgency from 2007 to 2009, which damaged the health infrastructure. An outbreak of dengue fever (DF) was declared in the valley in 2013. To investigate this outbreak, we established active surveillance of national hospitals and private clinics, reviewed available clinical and laboratory records, and conducted entomological survey. From August to November 2013, 16,000 suspected patients with acute febrile illness were presented to health facilities...
January 16, 2017: American Journal of Tropical Medicine and Hygiene
Hafiz Muhammad Ashraf, Muhammad Kashif Zahoor, Shabab Nasir, Humara Naz Majeed, Sarwat Zahoor
BACKGROUND: Keeping in view the havoc situation of dengue fever in Pakistan, the current study was designed to demonstrate the genetic variations, gene flow and rate of migration from Lahore and Faisalabad. METHODS: The larvae were collected from both natural and artificial breeding places from each collection site. The adult mosquitoes were collected by means of sweep net and battery-operated aspirator. DNA extraction was performed using TNE buffer method. Ten GeneLink-A series RAPD primers were used for PCR amplification and the data was analyzed through POPGENE...
December 2016: Journal of Arthropod-borne Diseases
Muhammad Suleman, Rani Faryal, Muhammad Masroor Alam, Salmaan Sharif, Shahzad Shaukat, Uzma Bashir Aamir, Adnan Khurshid, Mehar Angez, Massab Umair, Mian Muhammad Sufian, Yasir Arshad, Syed Sohail Zahoor Zaidi
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period...
March 2017: Annals of Laboratory Medicine
Sara Doosti, Mohammad Reza Yaghoobi-Ershadi, Francis Schaffner, Seyed Hassan Moosa-Kazemi, Kamran Akbarzadeh, Mohammad Mehdi Gooya, Hassan Vatandoost, Mohammad Reza Shirzadi, Ehsan Mosta-Favi
BACKGROUND: Epidemics of mosquito-borne viral infections such as dengue, chikungunya, West Nile and Rift Valley fevers in neighbouring countries and risk of introduction of exotic vectors into Iran have placed this country at a significant risk for these mosquito-borne diseases. METHODS: After the first dengue case reported in Iran in 2008, active entomological surveillance of Aedes albopictus (Skuse) and Ae. aegypti (Linnaeus) were conducted in May/Jun, Sep, and Oct/Nov, 2008-2014...
August 2016: Iranian Journal of Public Health
John M Humphrey, Natalie B Cleton, Chantal B E M Reusken, Marshall J Glesby, Marion P G Koopmans, Laith J Abu-Raddad
BACKGROUND: Dengue virus (DENV) infection is widespread and its disease burden has increased in past decades. However, little is known about the epidemiology of dengue in the Middle East and North Africa (MENA). METHODOLOGY / PRINCIPAL FINDINGS: Following Cochrane Collaboration guidelines and reporting our findings following PRISMA guidelines, we systematically reviewed available records across MENA describing dengue occurrence in humans (prevalence studies, incidence studies, and outbreak reports), occurrence of suitable vectors (Aedes aegypti and Aedes albopictus), and DENV vector infection rates...
December 2016: PLoS Neglected Tropical Diseases
Taranum Ruba Siddiqui, Saima Ghazal, Safia Bibi, Waquaruddin Ahmed, Shaimuna Fareeha Sajjad
BACKGROUND: Prevention is most effective in reducing dengue infection risk, especially in endemic countries like Pakistan. Evaluation of public awareness and health beliefs regarding dengue fever (DF) is important for devising disease control strategies. This study assessed dengue knowledge, health beliefs, and preventive practices against DF in different socioeconomic groups of Karachi, Pakistan. METHODOLOGY: In this community-based cross-sectional study, 6 randomly selected towns were visited, 2 persons (man and woman) per household were interviewed using a structured questionnaire, and household practices were observed...
November 2016: PLoS Neglected Tropical Diseases
Toqeer Ahmed, Miklas Scholz, Furat Al-Faraj, Wajeeha Niaz
Water-related impacts due to change in climatic conditions ranging from water scarcity to intense floods and storms are increasing in developing countries like Pakistan. Water quality and waterborne diseases like hepatitis, cholera, typhoid, malaria and dengue fever are increasing due to chaotic urbanization, industrialization, poor hygienic conditions, and inappropriate water management. The morbidity rate is high due to lack of health care facilities, especially in developing countries. Organizations linked to the Government of Pakistan (e...
October 27, 2016: International Journal of Environmental Research and Public Health
Suleman Atique, Shabbir Syed Abdul, Chien-Yeh Hsu, Ting-Wu Chuang
OBJECTIVE: To identify the influences of local and regional climate phenomena on dengue transmission in Lahore District of Pakistan, from 2006 to 2014. METHODS: Time-series models were applied to analyze associations between reported cases of dengue and climatic parameters. The coherence trend of regional climate phenomena (IOD and ENSO) was evaluated with wavelet analysis. RESULTS: The minimum temperature 4 months before the dengue outbreak played the most important role in the Lahore District (P = 0...
October 2016: Asian Pacific Journal of Tropical Medicine
Ajeet Kumar Gadhwal, B S Ankit, Chitresh Chahar, Pankaj Tantia, P Sirohi, R P Agrawal
OBJECTIVE: Thrombocytopenia in dengue fever is a common and serious complication. However, no specific treatment is available for dengue fever induced thrombocytopenia. In few countries (Pakistan, Malaysia, Sri Lanka and other Asian countries) the leaf extract of Carica papaya has been effectively used for thrombocytopenia. So, the study is planned to access effect of Carica papaya leaf extract on platelet count in dengue fever patients. METHODS: All participants were randomised into two groups, study group and control group; the study group was given papaya leaf extract capsule of 500 mg once daily and routine supportive treatment for consecutive five days...
June 2016: Journal of the Association of Physicians of India
Muhammad Sohail Afzal
In Pakistan which ranked second in terms of hepatitis C virus (HCV) infection, it is highly needed to have an established diagnostic test for antiviral therapy response prediction. Interleukin 28B (IL-28B) genetic testing is widely used throughout the world for interferon based therapy prediction for HCV patients and is quite helpful not only for health care workers but also for the patients. There is a strong relationship between single nucleotide polymorphisms at or near the IL-28B gene and the sustained virological response with pegylated interferon plus ribavirin treatment for chronic hepatitis C...
September 18, 2016: World Journal of Hepatology
Nazish Bostan, Sundus Javed, Nabgha-E-Amen, Syed Ali Musstjab Akber Shah Eqani, Faheem Tahir, Habib Bokhari
Dengue fever is regarded as one of the most prominent emerging arboviral infections in Pakistan since its first epidemic almost 2 decades ago. Interplay between potential vectors, susceptible host, and lax environmental conditions may promote the infection, leading to an epidemic. These factors may indeed have played a major role in the spread of the disease in the country, which was limited to Karachi till 2006. With recent natural disasters such as the earthquake in 2005 and flooding in 2010, 2011 and 2012, numbers of vector-borne diseases and outbreaks including dengue fever are on the rise in Pakistan...
September 6, 2016: Reviews in Medical Virology
Amjad Ali, Habib Ahmad, Muhammad Idrees, Fazli Zahir, Ijaz Ali
BACKGROUND: Dengue virus is circulating in Pakistan since 1994, which causes major and minor outbreaks in many areas of the country. The incidence of dengue in Pakistan in past years mainly restricted to parts of Sindh and Punjab provinces. As such, a severe dengue outbreak appeared in Pakistan in 2011, particularly in Punjab province with Lahore as the most hit city (290 deaths). In 2013, for the first time in the history of Pakistan, dengue outbreak erupted in Swat District, Khyber Pakhtunkhwa, which claimed more than 57 lives...
2016: Virology Journal
Muhammad Zubair, Muhammad Ashraf, Aitezaz Ahsan, Noor-Ul-Ain Nazir, Hina Hanif, Haider Ali Khan
Infections due to Dengue virus are widespread throughout the world. Disease starts with mild flu like sickness to a severe intricate condition which results in the death of the patient. Dengue illness has high morbidity and mortality in Pakistan as well as in other Asian countries. The Review article is a discourse analysis that explores the facts about the history, emergence and impact of dengue in Pakistan and other Asian countries. Data was collected from internet sources, mainly using Science Direct and PubMed...
July 2016: JPMA. the Journal of the Pakistan Medical Association
Nabeel Abdur Rehman, Shankar Kalyanaraman, Talal Ahmad, Fahad Pervaiz, Umar Saif, Lakshminarayanan Subramanian
Thousands of lives are lost every year in developing countries for failing to detect epidemics early because of the lack of real-time disease surveillance data. We present results from a large-scale deployment of a telephone triage service as a basis for dengue forecasting in Pakistan. Our system uses statistical analysis of dengue-related phone calls to accurately forecast suspected dengue cases 2 to 3 weeks ahead of time at a subcity level (correlation of up to 0.93). Our system has been operational at scale in Pakistan for the past 3 years and has received more than 300,000 phone calls...
July 2016: Science Advances
Saranjam Khan, Rahat Ullah, Asifullah Khan, Noorul Wahab, Muhammad Bilal, Mushtaq Ahmed
The current study presents the use of Raman spectroscopy combined with support vector machine (SVM) for the classification of dengue suspected human blood sera. Raman spectra for 84 clinically dengue suspected patients acquired from Holy Family Hospital, Rawalpindi, Pakistan, have been used in this study.The spectral differences between dengue positive and normal sera have been exploited by using effective machine learning techniques. In this regard, SVM models built on the basis of three different kernel functions including Gaussian radial basis function (RBF), polynomial function and linear functionhave been employed to classify the human blood sera based on features obtained from Raman Spectra...
June 1, 2016: Biomedical Optics Express
Ali Arslan, Hamayun Rashid Rathor, Muhammad Uzair Mukhtar, Shumaila Mushtaq, Adil Bhatti, Muhammad Asif, Israr Arshad, Jam Farooq Ahmad
BACKGROUND & OBJECTIVES: Dengue is one of the most common arthropod-borne viral diseases which is transmitted mainly by two vector species, Aedes aegypti (Linnaeus 1762) and Ae. albopictus (Skuse, 1894) worldwide. As there is no effective medicine and vaccine available, vector control remains the most effective measure to prevent its transmission and outbreak. The aim of the study was to confirm the co-occurrence of Aedes aegypti and Ae. albopictus populations in the different localities of Rawalpindi, Pakistan and examine their susceptibility status against different groups of insecticides...
April 2016: Journal of Vector Borne Diseases
Catherine S Todd, Ghulam Farooq Mansoor, Cyril Buhler, Habiburrahman Rahimi, Rohullah Zekria, Stefan Fernandez, Amy F W Mikhail, Paul T Scott, Samuel L Yingst
OBJECTIVE: To measure prevalence of prior/current Plasmodium vivax and Plasmodium falciparum (PV and PF), Brucella spp. (BR), dengue virus (DENV), Leishmania donovani (visceral leishmaniasis; VL), and Crimean-Congo hemorrhagic fever (CCHF) virus exposure among Afghan National Army (ANA) recruits. METHODS: Randomly chosen, nationally representative serum samples from consenting men aged 18-40 years and who were screened between February 2010 and January 2011 were tested, with ∼25 samples/province...
August 2016: Vector Borne and Zoonotic Diseases
Umar Saeed, Zahra Zahid Piracha
Pakistan is a developing country that has a population of 190 million people and faces a huge burden of viral diseases. Every year during monsoon season heavy rain fall and lack of disaster management skills potentially increase the transmission of waterborne diseases, vector borne diseases and viral outbreaks. Due to severe flooding, thousands of people lose their lives and millions are displaced each year. In most of the cases the children who lose their family members are forced into illegal professions of begging, child labor and prostitution which make them prone to sexually transmitted infections...
May 12, 2016: World Journal of Virology
Carmen Koo, Simrandeep Kaur, Zhi-Yong Teh, Helen Xu, Amna Nasir, Yee-Ling Lai, Erum Khan, Lee-Ching Ng, Hapuarachchige C Hapuarachchi
Dengue fever is currently the most prevalent disease caused by mosquito-borne flaviviruses. Despite being potentially fatal, there are no specific antiviral therapies for Dengue virus (DENV) infections. Therefore, early, accurate, and rapid diagnosis plays an important role in proper patient management. In this study, we evaluated the performance of a probe-based real-time RT-PCR (rRT-PCR) assay against that of a conventional RT-PCR assay in three sample cohorts from Pakistan (n = 94) and Singapore (first cohort; n = 559, second cohort; n = 123)...
July 2016: Vector Borne and Zoonotic Diseases
Sherif A El-Kafrawy, Sayed S Sohrab, Said Abol Ela, Adly M M Abd-Alla, Rowa Alhabbab, Suha A Farraj, Norah A Othman, Ahmed M Hassan, Max Bergoin, Raphaelle Klitting, Remi N Charrel, Anwar M Hashem, Tariq A Madani, Esam I Azhar
INTRODUCTION: Dengue is a significant arboviral infection that represents a major public health concern worldwide. The infection is endemic in most parts of South East Asia, sub-Saharan Africa, and Latin America. Among the four dengue virus (DENV) serotypes, DENV-2 has been reported to be the predominant serotype in Saudi Arabia since 1992. However, virological and epidemiological data of DENV-2 from Saudi Arabia are severely deficient and require further investigations. METHODS: Full genome sequencing of a recent DENV-2 isolate and phylogenetic analysis of all available DENV-2 sequences from Saudi Arabia...
June 2016: Vector Borne and Zoonotic Diseases
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