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https://www.readbyqxmd.com/read/28754252/detection-of-selected-arboviral-infections-in-patients-with-history-of-persistent-fever-in-pakistan
#1
Tahir Yaqub, Muhammad Zubair Shabbir, Nadia Mukhtar, Zarfishan Tahir, Tariq Abbas, Ehab Amir, Gabriel Defang
Surveillance is a valuable tool for understanding prevailing and previously undiagnosed infections in a geographic area. We examined 480 archived serum samples from patients with history of persistent fever (>40°C, 60-72h) who were referred to hospitals in Rawalpindi/Islamabad, Lahore, and Faisalabad districts for dengue antibody detection in 2014-15. Each sample was processed for detection of antigens and seroconversion, using real-time polymerase chain reaction and enzyme linked immunosorbent assay, respectively, against dengue haemorrhagic fever (DHF) virus serotypes 1-4, West Nile virus fever (WNVF), Crimean-Congo haemorrhagic fever (CCHF), and Chikungunya virus (CGV)...
July 25, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28719296/analysis-of-dengue-serotype-4-in-sri-lanka-during-the-2012-2013-dengue-epidemic
#2
Anna Uehara, Hasitha Aravinda Tissera, Champica K Bodinayake, Ananda Amarasinghe, Ajith Nagahawatte, L Gayani Tillekeratne, Jie Cui, Megan E Reller, Paba Palihawadana, Sunethra Gunasena, Aruna Dharshan Desilva, Annelies Wilder-Smith, Duane J Gubler, Christopher W Woods, October M Sessions
The four serotypes of dengue virus (DENV-1, -2, -3, and -4) have had a rapidly expanding geographic range and are now endemic in over 100 tropical and subtropical countries. Sri Lanka has experienced periodic dengue outbreaks since the 1960s, but since 1989 epidemics have become progressively larger and associated with more severe disease. The dominant virus in the 2012 epidemic was DENV-1, but DENV-4 infections were also commonly observed. DENV-4 transmission was first documented in Sri Lanka when it was isolated from a traveler in 1978, but has been comparatively uncommon since dengue surveillance began in the early 1980s...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28636447/emerging-viral-infections-in-pakistan-issues-concerns-and-future-prospects
#3
Ali Talha Khalil, Muhammad Ali, Faouzia Tanveer, Muhammad Ovais, Muhammad Idrees, Zabta Khan Shinwari, James E Hollenbeck
Emerging infectious diseases pose a serious threat to public health security; this is especially true in the underdeveloped world because of limited resources to combat them. These emerging pathogens are characterized by a novel mode of pathogenesis and, in some cases, a broad host range. Over the past few decades, Pakistan has suffered a great deal from infectious diseases such as dengue, Crimean-Congo fever, hepatitis, measles, and polio. Changing climate conditions, environmental degradation, global warming, loss of biodiversity, and other ecological determinants have a direct effect on these diseases and result in the emergence and reemergence of infectious entities...
May 2017: Health Security
https://www.readbyqxmd.com/read/28586575/and-yet-one-more-adds-to-the-sorrow
#4
EDITORIAL
Muhammad Zeeshan Haroon, Mohammad Salim Wazir
Chikungunya is one of the vector borne diseases. It is caused by Chikungunya virus, an alpha virus, spread by the bite of female Aedes mosquito. Chikungunya is a non-fatal, self-limiting Dengue like illness which is characterized by high grade fever, headache, skin rash, and prolong debilitating arthralgia mostly affecting peripheral small joints. Treatment is usually supportive for the symptoms which includes antipyretic, analgesics and anti-inflammatory drugs. Current outbreak in Karachi is the first declared outbreak in Pakistan...
October 2016: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/28552190/spatial-mapping-of-temporal-risk-to-improve-prevention-measures-a-case-study-of-dengue-epidemic-in-lahore
#5
Sidrah Hafeez, Muhammad Amin, Bilal Ahmed Munir
BACKGROUND: Dengue is identified as serious vector born infectious disease by WHO, threating around 2.5 billion people around the globe. Pakistan is facing dengue epidemic since 1994 but 2010 and 2011 dengue outbreaks were worst. During 2011 dengue outbreak 22,562 cases were reported and 363 died due to this fatal infection in Pakistan. In this study, Lahore District was chosen as it was severely affected in 2011 dengue outbreak with 14,000 reported cases and 300 deaths. There is no vaccine developed yet for the disease control, so only effective early warning, prevention and control measures can reduce the potential disease risk...
June 2017: Spatial and Spatio-temporal Epidemiology
https://www.readbyqxmd.com/read/28420913/increasing-usage-of-rapid-diagnostics-for-dengue-virus-detection-in-pakistan
#6
Zahra Hasan, Saher Razzak, Muhammad Farhan, Maryam Rahim, Nazneen Islam, Azra Samreen, Erum Khan
OBJECTIVE: To evaluate the trends in usage of dengue virus diagnostics in Pakistan. METHODS: This retrospective study was conducted at the Aga Khan University Hospital, Karachi, and comprised data for specimens tested for dengue virus from January 2012 to December 2015. Test for dengue virus ribonucleic acid by reverse transcription polymerase chain reaction, dengue virus antigen by immunochromatic assay and for human immunoglobulin M against dengue virus by enzyme-linked immunosorbent assay were reviewed...
April 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28367194/evaluation-of-non-structural-protein-1-ns1-positive-patients-of-2013-dengue-outbreak-in-khyber-pakhtunkhwa-pakistan
#7
Ghosia Lutfullah, Jawad Ahmed, Aftab Khan, Hina Ihsan, Jamshed Ahmad
BACKGROUND & OBJECTIVE: Dengue infection is an arthropod borne disease caused by Dengue virus in humans. Dengue virus infection has more potential to produce severe form of the disease with more severe symptoms. Proper diagnosis of dengue fever is very important for its safe management. The objective of this study was to evaluate the non structural protein-1 (NS1) positive parameter for identification of dengue fever by using ELISA from 2013 dengue outbreak in Khyber Pakhtunkhwa. METHODS: It was a cross sectional study conducted among 384 patients tested for dengue admitted to different hospitals of Khyber Pakhtunkhwa April to December 2013 with symptoms related to classical dengue fever...
January 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28353206/assessing-spatio-temporal-trend-of-vector-breeding-and-dengue-fever-incidence-in-association-with-meteorological-conditions
#8
Afifa Malik, Abdullah Yasar, Amtul Bari Tabinda, Ihsan Elahi Zaheer, Khalida Malik, Adeeba Batool, Yusra Mahfooz
Th aim of this study is to investigate spatio-temporal trends of dengue vector breeding and epidemic (disease incidence) influenced by climatic factors. The spatio-temporal (low-, medium-, and high-intensity periods) evaluation of entomological and epidemiological investigations along with climatic factors like rainfall (RF), temperature (Tmax), relative humidity (RH), and larval indexing was conducted to develop correlations in the area of Lahore, Pakistan. The vector abundance and disease transmission trend was geo-tagged for spatial insight...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28303992/predicting-dengue-outbreak-in-the-metropolitan-city-lahore-pakistan-using-dengue-vector-indices-and-selected-climatological-variables-as-predictors
#9
Ejaz Mahmood Ahmad Qureshi, Amtul Bari Tabinda, Seemal Vehra
OBJECTIVE: Dengue viruses have rapidly spread in Southeast Asia, particularly in Pakistan, during the last few years, so the present study was undertaken to formulate a model for predicting dengue outbreak. METHODOLOGY: A panel data using STRATA statistical software was employed to determine the effects of entomological indices, Per Man Hour Density (PMHD), Minimum Infection Rate (MIR) and selected climatic variables on dengue patients by using Fixed Effects (FE) and Random Effects (RE) models...
March 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28291567/demographic-characteristics-of-dengue-virus-outbreaks-in-khyber-pakhtunkhwa-province-pakistan-during-2003-2015
#10
Muhammad Suleman, Rani Faryal, Muhammad Masroor Alam, Syed Sohail Zahoor Zaidi
No abstract text is available yet for this article.
March 10, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28274279/-preliminary-seroepidemiological-survey-of-dengue-infections-in-pakistan-2009-2014
#11
Muhammad Suleman, Hyeong-Woo Lee, Syed Sohail Zahoor Zaidi, Muhammad Masroor Alam, Nadia Nisar, Uzma Bashir Aamir, Salmaan Sharif, Shahzad Shaukat, Adnan Khurshid, Mehar Angez, Massab Umair, Ghulam Mujtaba, Rani Faryal
BACKGROUND: Dengue virus is the causative agent of dengue fever, a vector borne infection which causes self-limiting to life threatening disease in humans. A sero-epidemiological study was conducted to understand the current epidemiology of dengue virus in Pakistan which is now known as a dengue endemic country after its first reported outbreak in 1994. METHODS: To investigate the prevalence of dengue virus in Pakistan during 2009-2014, a total of 9,493 blood samples were screened for the detection of anti-dengue IgM antibodies using ELISA...
March 9, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28219668/outbreak-of-dengue-virus-type-3-in-malakand-pakistan-2015-a-laboratory-perspective
#12
Muhammad Suleman, Rani Faryal, Muhammad Masroor Alam, Adnan Khurshid, Salmaan Sharif, Shahzad Shaukat, Mehar Angez, Massab Umair, Mian Muhammad Sufian, Yasir Arshad, Tanveer Inam, Syed Sohail Zahoor Zaidi
An outbreak of dengue fever was reported in Malakand district, Khyber Pakhtunkhwa (KP) province of Pakistan during 2015. Detection of viral RNA by real-time PCR confirmed dengue virus serotype-3 (DENV-3) to be the causative agent causing the outbreak. Phylogenetic analysis based on partial E-NS1 gene sequences showed that the DENV-3 viruses belonged to genotype III with maximum homology with the dengue-3 strains previously reported from Pakistan and India. Our current report provides updated information on molecular epidemiology and phylogenetic analysis of dengue virus serotypes responsible for 2015 outbreak in KP...
May 2017: Acta Tropica
https://www.readbyqxmd.com/read/28093537/dengue-epidemic-in-postconflict-swat-district-khyber-pakhtunkhwa-pakistan-2013
#13
Mamoona Chaudhry, Saeed Ahmad, Hamad Bin Rashid, Iftikhar Ud Din
AbstractSwat, a lush green valley of 1.3 million persons, remained under militant insurgency from 2007 to 2009, which damaged the health infrastructure. An outbreak of dengue fever (DF) was declared in the valley in 2013. To investigate this outbreak, we established active surveillance of national hospitals and private clinics, reviewed available clinical and laboratory records, and conducted entomological survey. From August to November 2013, 16,000 suspected patients with acute febrile illness were presented to health facilities...
April 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28032107/genetic-analysis-of-aedes-aegypti-using-random-amplified-polymorphic-dna-rapd-markers-from-dengue-outbreaks-in-pakistan
#14
Hafiz Muhammad Ashraf, Muhammad Kashif Zahoor, Shabab Nasir, Humara Naz Majeed, Sarwat Zahoor
BACKGROUND: Keeping in view the havoc situation of dengue fever in Pakistan, the current study was designed to demonstrate the genetic variations, gene flow and rate of migration from Lahore and Faisalabad. METHODS: The larvae were collected from both natural and artificial breeding places from each collection site. The adult mosquitoes were collected by means of sweep net and battery-operated aspirator. DNA extraction was performed using TNE buffer method. Ten GeneLink-A series RAPD primers were used for PCR amplification and the data was analyzed through POPGENE...
December 2016: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/28029002/dengue-virus-serotypes-circulating-in-khyber-pakhtunkhwa-province-pakistan-2013-2015
#15
Muhammad Suleman, Rani Faryal, Muhammad Masroor Alam, Salmaan Sharif, Shahzad Shaukat, Uzma Bashir Aamir, Adnan Khurshid, Mehar Angez, Massab Umair, Mian Muhammad Sufian, Yasir Arshad, Syed Sohail Zahoor Zaidi
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period...
March 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/27928533/mosquito-surveillance-and-the-first-record-of-the-invasive-mosquito-species-aedes-stegomyia-albopictus-skuse-diptera-culicidae-in-southern-iran
#16
Sara Doosti, Mohammad Reza Yaghoobi-Ershadi, Francis Schaffner, Seyed Hassan Moosa-Kazemi, Kamran Akbarzadeh, Mohammad Mehdi Gooya, Hassan Vatandoost, Mohammad Reza Shirzadi, Ehsan Mosta-Favi
BACKGROUND: Epidemics of mosquito-borne viral infections such as dengue, chikungunya, West Nile and Rift Valley fevers in neighbouring countries and risk of introduction of exotic vectors into Iran have placed this country at a significant risk for these mosquito-borne diseases. METHODS: After the first dengue case reported in Iran in 2008, active entomological surveillance of Aedes albopictus (Skuse) and Ae. aegypti (Linnaeus) were conducted in May/Jun, Sep, and Oct/Nov, 2008-2014...
August 2016: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/27926925/dengue-in-the-middle-east-and-north-africa-a-systematic-review
#17
REVIEW
John M Humphrey, Natalie B Cleton, Chantal B E M Reusken, Marshall J Glesby, Marion P G Koopmans, Laith J Abu-Raddad
BACKGROUND: Dengue virus (DENV) infection is widespread and its disease burden has increased in past decades. However, little is known about the epidemiology of dengue in the Middle East and North Africa (MENA). METHODOLOGY / PRINCIPAL FINDINGS: Following Cochrane Collaboration guidelines and reporting our findings following PRISMA guidelines, we systematically reviewed available records across MENA describing dengue occurrence in humans (prevalence studies, incidence studies, and outbreak reports), occurrence of suitable vectors (Aedes aegypti and Aedes albopictus), and DENV vector infection rates...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27832074/use-of-the-health-belief-model-for-the-assessment-of-public-knowledge-and-household-preventive-practices-in-karachi-pakistan-a-dengue-endemic-city
#18
Taranum Ruba Siddiqui, Saima Ghazal, Safia Bibi, Waquaruddin Ahmed, Shaimuna Fareeha Sajjad
BACKGROUND: Prevention is most effective in reducing dengue infection risk, especially in endemic countries like Pakistan. Evaluation of public awareness and health beliefs regarding dengue fever (DF) is important for devising disease control strategies. This study assessed dengue knowledge, health beliefs, and preventive practices against DF in different socioeconomic groups of Karachi, Pakistan. METHODOLOGY: In this community-based cross-sectional study, 6 randomly selected towns were visited, 2 persons (man and woman) per household were interviewed using a structured questionnaire, and household practices were observed...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27801802/water-related-impacts-of-climate-change-on-agriculture-and-subsequently-on-public-health-a-review-for-generalists-with-particular-reference-to-pakistan
#19
REVIEW
Toqeer Ahmed, Miklas Scholz, Furat Al-Faraj, Wajeeha Niaz
Water-related impacts due to change in climatic conditions ranging from water scarcity to intense floods and storms are increasing in developing countries like Pakistan. Water quality and waterborne diseases like hepatitis, cholera, typhoid, malaria and dengue fever are increasing due to chaotic urbanization, industrialization, poor hygienic conditions, and inappropriate water management. The morbidity rate is high due to lack of health care facilities, especially in developing countries. Organizations linked to the Government of Pakistan (e...
October 27, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27794388/meteorological-influences-on-dengue-transmission-in-pakistan
#20
Suleman Atique, Shabbir Syed Abdul, Chien-Yeh Hsu, Ting-Wu Chuang
OBJECTIVE: To identify the influences of local and regional climate phenomena on dengue transmission in Lahore District of Pakistan, from 2006 to 2014. METHODS: Time-series models were applied to analyze associations between reported cases of dengue and climatic parameters. The coherence trend of regional climate phenomena (IOD and ENSO) was evaluated with wavelet analysis. RESULTS: The minimum temperature 4 months before the dengue outbreak played the most important role in the Lahore District (P = 0...
October 2016: Asian Pacific Journal of Tropical Medicine
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