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https://www.readbyqxmd.com/read/29028070/gene-expression-in-human-adipose-derived-stem-cells-comparison-of-2d-films-3d-electrospun-meshes-or-co-cultured-scaffolds-with-two-way-paracrine-effects
#1
S C Hess, W J Stark, D Mohn, N Cohrs, S Märsmann, M Calcagni, P Cinelli, J Buschmann
Finding the appropriate cues to trigger the desired differentiation is a challenge in tissue engineering when stem cells are involved. In this regard, three-dimensional environments are often compared to cells' two-dimensional culture behaviour (plastic culture dish). Here, we compared the gene expression pattern of human adipose-derived stem cells (ASC) seeded in a three-dimensional (3D) electrospun mesh and on a two-dimensional (2D) film - both of exactly the same material. Additionally, we conducted experiments with a scaffold floating above a film to investigate two-way paracrine effects (co-system)...
October 13, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28855134/antioxidant-and-anti-aging-effects-of-acidic-extractable-polysaccharides-by-agaricus-bisporus
#2
Shangshang Li, Hui Liu, Wenshuai Wang, Xiuxiu Wang, Chen Zhang, Jianjun Zhang, Huijuan Jing, Zhenzhen Ren, Zheng Gao, Xinling Song, Le Jia
This study was designed to investigate the antioxidant and anti-aging effects of D-galatose-induced (D-gal-induced) aging mice as well as monosaccharide compositions of acidic-extractable polysaccharides (AcAPS) and its major purified fractions (AcAPS-1, AcAPS-2 and AcAPS-3) from the fruiting body of Agaricus bisporus. In the in vitro assays, AcAPS-2 showed superior scavenging activities on hydroxyl (82.98±4.67%) and DPPH (64.47±4.05%) radicals at the concentration of 1.0mg/mL than AcAPS and other polysaccharides fractions...
August 27, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28811865/oxidative-biochemistry-disbalance-and-changes-on-proteomic-profile-in-salivary-glands-of-rats-induced-by-chronic-exposure-to-methylmercury
#3
Leonardo Oliveira Bittencourt, Bruna Puty, Senda Charone, Walessa Alana Bragança Aragão, Paulo Mecenas Farias-Junior, Marcia Cristina Freitas Silva, Maria Elena Crespo-Lopez, Aline de Lima Leite, Marilia Afonso Rabelo Buzalaf, Rafael Rodrigues Lima
Methylmercury (MeHg) is one of the most toxic mercury species, which can cause many systemic damages, but little is known about its effect in the salivary glands. This study aimed to analyze the mercury levels, oxidative stress, and proteomic profile in parotid, submandibular, and sublingual salivary glands of rats, after chronic MeHg intoxication. Two groups of twenty male Wistar rats (90 days of age) were used on the experiment. MeHg group was intoxicated by intragastric gavage with MeHg at a dose of 0.04 mg/kg/day for 60 days, while the control group received only oil...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28746612/oxidative-stress-responses-of-juvenile-tambaqui-colossoma-macropomum-after-short-term-anesthesia-with-benzocaine-and-ms-222
#4
Giovanna R Stringhetta, Luis A L Barbas, Lucas C Maltez, Luís A Sampaio, José M Monserrat, Luciano O Garcia
The present study aimed to evaluate the effects of benzocaine and tricaine methanesulfonate on oxidative stress parameters of juvenile tambaqui tissues. Fish (n=80) were anesthetized with benzocaine (100 mg L-1) or tricaine (240 mg L-1) and two control groups were used (non-anesthetized fish and fish exposed to ethanol-only). After anesthetic induction 10 fish/anesthetic were euthanized after 3, 12 and 24 hours post-anesthesia and tissue samplings (gills, liver and brain) were performed. Samples were submitted to analyses of enzyme activity glutathione-S-transferase (GST), cellular lipid peroxidation (TBARS) and total antioxidant capacity (ACAP)...
July 24, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28746240/-does-the-organization-of-plastic-surgery-units-into-independent-departments-affect-academic-productivity
#5
Scott N Loewenstein, Stephen Duquette, Nakul Valsangkar, Umakanth Avula, Neha Lad, Juan Socas, Roberto L Flores, Rajiv Sood, Leonidas G Koniaris
BACKGROUND: There is an increased push for plastic surgery units in the United States to become independent departments administered autonomously rather than as divisions of a multispecialty surgery department. The purpose of this research was to determine if there are any quantifiable differences in the academic performance of departments versus divisions. METHODS: Using a list of the plastic surgery units affiliated with The American Council of Academic Plastic Surgeons (ACAPS), unit websites were queried for departmental status and to obtain a list of affiliated faculty...
July 12, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28726029/ammonia-exposure-and-subsequent-recovery-trigger-oxidative-stress-responses-in-juveniles-of-brazilian-flounder-paralichthys-orbignyanus
#6
Lucas Campos Maltez, Giovanna Rodrigues Stringhetta, Alain Danilo Enamorado, Marcelo Hideo Okamoto, Luis Alberto Romano, José María Monserrat, Luís André Sampaio, Luciano Garcia
The effects of ammonia exposure and recovery on oxidative stress parameters and histology of juvenile Brazilian flounder Paralichthys orbignyanus were evaluated. The fish were exposed to 0.12, 0.28 and 0.57 mg NH3-N L(-1), plus a control, for 10 days followed by the same recovery time in ammonia-free water. Gill, liver and muscle samples (n = 9) were collected after 1, 5 and 10 days of exposure and after recovery for oxidative stress analysis (antioxidant capacity against peroxyl radicals (ACAP); glutathione S-transferase (GST) activity; lipoperoxidation levels measured through thiobarbituric acid reactive substances (TBARS) content)...
December 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28709126/effects-of-increasing-temperature-alone-and-combined-with-copper-exposure-on-biochemical-and-physiological-parameters-in-the-zooxanthellate-scleractinian-coral-mussismilia-harttii
#7
Juliana da Silva Fonseca, Laura Fernandes de Barros Marangoni, Joseane Aparecida Marques, Adalto Bianchini
Effects of increasing temperature alone and in combination with exposure to dissolved copper (Cu) were evaluated in the zooxanthellate scleractinian coral Mussismilia harttii using a marine mesocosm system. Endpoints analyzed included parameters involved in metabolism [maximum photosynthetic capacity of zooxanthellae (Fv/Fm), chlorophyll a and ATP concentrations], calcification [carbonic anhydrase (CA) and Ca(2+)-Mg(2+)-ATPase activity], and oxidative status [antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (LPO)]...
September 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28689713/antioxidant-defense-system-and-oxidative-status-in-antarctic-fishes-the-sluggish-rockcod-notothenia-coriiceps-versus-the-active-marbled-notothen-notothenia-rossii
#8
Roberta Daniele Klein, Carlos Eduardo Rosa, Elton Pinto Colares, Ricardo Berteaux Robaldo, Pablo Elias Martinez, Adalto Bianchini
Adaptive responses of antioxidant defense systems (ADS) to changes in increased levels of activity are critical, especially in Antarctic fishes. The benthopelagic marbled notothen (Notothenia rossii) shows higher spontaneous activity than the benthonic and sluggish rockcod (N. coriiceps). Therefore, we hypothesize that species-related responses of ADS would occur to counteract different rates of reactive oxygen species formation in these two Antarctic fish. Here we evaluated ADS and oxidative damage in tissues (brain, gills, liver and white muscle) of the two Antarctic fish...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28689712/effects-of-increasing-temperature-on-antioxidant-defense-system-and-oxidative-stress-parameters-in-the-antarctic-fish-notothenia-coriiceps-and-notothenia-rossii
#9
Roberta Daniele Klein, Vinicius Dias Borges, Carlos Eduardo Rosa, Elton Pinto Colares, Ricardo Berteaux Robaldo, Pablo Elias Martinez, Adalto Bianchini
Antioxidant defense system (ADS) and oxidative stress parameters were evaluated in the Antarctic fish Notothenia rossii and N. coriiceps exposed to increasing temperature. Acclimated fish were kept at 0°C or exposed to 4°C for 1day (N. rossii) or to 2 and 4°C for 1 and 6 days (N. coriiceps). Measurements were assessed in brain, gills, liver, white muscle and erythrocytes. Parameters analyzed included antioxidant capacity against peroxyl radicals (ACAP); reduced glutathione (GSH) and metallothionein-like proteins (MTLP) concentration; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutamate-cysteine ligase (GCL) activity; lipid peroxidation (LPO) level and protein carbonyl (PC) concentration...
August 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28672897/adult-community-acquired-pneumonia-with-unusually-enlarged-mediastinal-lymph-nodes-a-case-report
#10
Lanhua Zhang, Shixiong Qiu, Cui Tang, Jinming Xu
Adult community-acquired pneumonia (ACAP) is the most prevalent pulmonary infectious disease that may be asymptomatic or have varying clinical presentations. Patients with ACAP often present with enlarged mediastinal lymph nodes on their chest computed tomography images. However, large irregular swollen lymph nodes are rarely reported in ACAP, and may therefore be confused with enlarged lymph node masses. In the present case report, the patient presented with lymph node masses, which were ameliorated to their normal size following antimicrobial treatment...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28646092/deletion-analysis-of-agd1-reveals-domains-crucial-for-its-plasma-membrane-recruitment-and-function-in-root-hair-polarity
#11
Cheol-Min Yoo, Satoshi Naramoto, J Alan Sparks, Bibi Rafeiza Khan, Jin Nakashima, Hiroo Fukuda, Elison B Blancaflor
AGD1, a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP), functions in specifying root hair polarity in Arabidopsis thaliana To better understand how AGD1 modulates root hair growth, we generated full length and domain-deleted AGD1-green fluorescent protein (GFP) constructs, and followed their localization during root hair development. AGD1-GFP localized to the cytoplasm and was recruited to specific regions of the root hair plasma membrane (PM). Distinct PM AGD1-GFP signal was first detected along the site of root hair bulge formation...
June 23, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28584202/feasibility-of-atrial-autocapture%C3%A2-to-detect-atrial-evoked-response-experience-from-102-patients-implanted-with-dual-chamber-pacemakers
#12
Hai-Long Si, Qin Qin, Bing-Rang Zhao, Gang Chen, Ya-Ru Lu, Lu Kou, Jing-Yu Yang, Wen-Hua Lin, Zi-Wen Ren
BACKGROUND: Atrial AutoCapture™ (ACap™) was a new technological development that confirmed atrial capture by analyzing evoked response (ER) with a new method - paced depolarization integral ER detection - and optimized energy output to changes in the stimulation threshold. The purpose of this study was to evaluate the clinical performance of ACap™ function. METHODS: This was a prospective, observational, nonrandomized two-center study. Between November 2008 and August 2014, 102 patients were enrolled from two different institutions...
June 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28571103/epidemiology-and-outcomes-of-missing-admission-medication-history-in-severe-trauma-a-retrospective-study
#13
Matthew Miller, Richard Morris, Nicoletta Fisicaro, Kate Curtis
OBJECTIVE: Anticoagulant and antiplatelet (ACAP) drugs are associated with increased mortality in trauma patients, therefore medication history on admission is important. Whether these medications are recorded on trauma admission has not been investigated, nor if absence of a medication history is associated with worse patient outcomes. METHODS: We conducted a retrospective database review combining demographic and outcome data from the St George Hospital (Sydney) trauma registry with admission medication history in the electronic record...
June 1, 2017: Emergency Medicine Australasia: EMA
https://www.readbyqxmd.com/read/28541850/repeat-head-ct-not-necessary-for-patients-with-a-negative-initial-head-ct-on-anticoagulation-or-antiplatelet-therapy-suffering-low-altitude-falls
#14
Zachary M Bauman, John M Ruggero, Sunny Squindo, Chris McEachin, Michelle Jaskot, Will Ngo, Scott Barnes, Peter P Lopez
Anticoagulation and antiplatelet (ACAP) medications are increasingly prescribed to patients at high risk for falls. Many trauma centers have developed protocols for obtaining repeat head CT (HCT) for patients with low-altitude falls on ACAP therapy. We assess the need for routine scheduled repeat HCT in this population. Prospective, observational analysis of all low-altitude fall (<6 feet) patients on ACAP therapy evaluated at a Level II trauma center. All low-altitude fall patients with visible or suspected head trauma received an initial HCT...
May 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28470376/sublethal-effects-in-perinereis-gualpensis-polychaeta-nereididae-exposed-to-mercury-pyrene-sediment-mixture-observed-in-a-multipolluted-estuary
#15
M Díaz-Jaramillo, K S B Miglioranza, P Carriquiriborde, D Marino, C N Pegoraro, G Valenzuela, R Barra
Sediment-living organisms can be subjected to a multi-pollution condition due to an increase in the diversity of contaminants. Sediment mixtures of Mercury (Hg) and some polycyclic aromatics hydrocarbons like Pyrene (Pyr) are common in heavily industrialized coastal zones. In the present study, greater than (>) and less than (<) probable effect concentration levels (PELs) of Hg and Pyr were assessed using spiked sediments in order to determine combined (Hg + Pyr) effects in uptake, metabolization and oxidative balance in the polychaete Perinereis gualpensis at short and medium-term exposure...
August 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28416982/automatic-atrial-capture-device-control-in-real-life-practice-a-multicenter-experience
#16
Massimo Giammaria, Gianluca Quirino, Mariangela Alberio, Umberto Parravicini, Eliana Cipolla, Guido Rossetti, Antonio Ruocco, Gaetano Senatore, Francesco Rametta, Paolo Pistelli
BACKGROUND: Device-based fully automatic pacing capture detection is useful in clinical practice and important in the era of remote care management. The main objective of this study was to verify the effectiveness of the new ACAP Confirm® algorithm in managing atrial capture in the medium term in comparison with early post-implantation testing. METHODS: Data were collected from 318 patients (66% male; mean age, 73±10 years); 237 of these patients underwent device implantation and 81 box changes in 31 Italian hospitals...
April 2017: Journal of Arrhythmia
https://www.readbyqxmd.com/read/28009599/a-national-curriculum-of-fundamental-skills-for-plastic-surgery-residency-report-of-the-inaugural-acaps-boot-camp
#17
EDITORIAL
Edward H Davidson, Jenny C Barker, Francesco M Egro, Alexandra Krajewski, Jeffrey E Janis, Vu T Nguyen
BACKGROUND: The Inaugural American Council of Academic Plastic Surgeons Plastic Surgery Boot Camp program was developed in response to ongoing changes in graduate medical education. The Boot Camp is a hands-on, practicum-based, 3-day course to introduce core concepts in plastic surgery for new plastic surgery residents (in both integrated and independent tracks). METHODS: The course was held in Pittsburgh in July to August 2015. There were 43 attendees (35 integrated/8 independent) representing 22 residency programs across 15 states...
February 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/27916764/serotonergic-system-does-not-contribute-to-the-hypothermic-action-of-acetaminophen
#18
Akihiro Fukushima, Wakana Sekiguchi, Kizuku Mamada, Yumi Tohma, Hideki Ono
Acetaminophen (AcAP), a widely-used antipyretic and analgesic drug, has been considered to exert its effects via central mechanisms, and many studies have demonstrated that the analgesic action of AcAP involves activation of the serotonergic system. Although the serotonergic system also plays an important role in thermoregulation, the contribution of serotonergic activity to the hypothermic effect of AcAP has remained unclear. In the present study, we examined whether the serotonergic system is involved in AcAP-induced hypothermia...
February 1, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27906609/glyphosate-adversely-affects-danio-rerio-males-acetylcholinesterase-modulation-and-oxidative-stress
#19
Fernanda Moreira Lopes, Sergiane Souza Caldas, Ednei Gilberto Primel, Carlos Eduardo da Rosa
It has been demonstrated that glyphosate-based herbicides are toxic to animals. In the present study, reactive oxygen species (ROS) generation, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO), as well as the activity and expression of the acetylcholinesterase (AChE) enzyme, were evaluated in Danio rerio males exposed to 5 or 10 mg/L of glyphosate for 24 and 96 h. An increase in ACAP in gills after 24 h was observed in the animals exposed to 5 mg/L of glyphosate. A decrease in LPO was observed in brain tissue of animals exposed to 10 mg/L after 24 h, while an increase was observed in muscle after 96 h...
April 2017: Zebrafish
https://www.readbyqxmd.com/read/27783013/medical-student-mentorship-in-plastic-surgery-the-mentor-s-perspective
#20
COMPARATIVE STUDY
Jeffrey E Janis, Jenny C Barker
BACKGROUND: The reproducible benefits of mentoring to mentees have been studied extensively. However, insights from the mentor perspective are less well described. This study evaluates mentorship of plastic surgery medical students from the attending surgeon's perspective. A comparison is made with a previous publication evaluating mentorship from the medical student's perspective. METHODS: An electronic survey was sent to 1025 active members of the American Council of Academic Plastic Surgeons (ACAPS) and the American Association of Plastic Surgeons (AAPS), with a combined response rate of 23 percent...
November 2016: Plastic and Reconstructive Surgery
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