Cassandra E Gheorghe, Sarah-Jane Leigh, Gabriel S S Tofani, Thomaz F S Bastiaanssen, Joshua M Lyte, Elisa Gardellin, Ashokkumar Govindan, Conall Strain, Sonia Martinez-Herrero, Michael S Goodson, Nancy Kelley-Loughnane, John F Cryan, Gerard Clarke
Chronic stress disrupts microbiota-gut-brain axis function and is associated with altered tryptophan metabolism, impaired gut barrier function, and disrupted diurnal rhythms. However, little is known about the effects of acute stress on the gut and how it is influenced by diurnal physiology. Here, we used germ-free and antibiotic-depleted mice to understand how microbiota-dependent oscillations in tryptophan metabolism would alter gut barrier function at baseline and in response to an acute stressor. Cecal metabolomics identified tryptophan metabolism as most responsive to a 15-min acute stressor, while shotgun metagenomics revealed that most bacterial species exhibiting rhythmicity metabolize tryptophan...
April 11, 2024: Cell Reports