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https://www.readbyqxmd.com/read/29044423/cultured-bovine-embryo-biopsy-conserves-methylation-marks-from-original-embryo
#1
Noelia Fonseca Balvís, Soledad Garcia-Martinez, Serafín Pérez-Cerezales, Elena Ivanova, Isabel Gomez-Redondo, Meriem Hamdi, Dimitrios Rizos, Pilar Coy, Gavin Kelsey, Alfonso Gutierrez-Adan
A major limitation of embryo epigenotyping by chromatin immunoprecipitation analysis is the reduced amount of sample available from an embryo biopsy. We developed an in vitro system to expand trophectoderm cells from an embryo biopsy to overcome this limitation. This work analyzes whether expanded trophectoderm (EX) is representative of the trophectoderm (TE) methylation or adaptation to culture has altered its epigenome. We took a small biopsy from the trophectoderm (30-40 cells) of in vitro produced bovine-hatched blastocysts and cultured it on fibronectin-treated plates until we obtained ∼4 × 104 cells...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29044303/immediate-postoperative-of-bariatric-surgery-in-the-intensive-care-unit-versus-an-inpatient-unit-a-retrospective-study-with-828-patients
#2
Guilherme Loures de Araújo Penna, Igor Pedreira Vaz, Eduardo Côrtes Fonseca, Marcelo Kalichsztein, Gustavo Freitas Nobre
OBJECTIVE: To compare the incidence of complications and the duration of hospitalization of patients undergoing bariatric surgery admitted to the intensive care unit or a post-surgical hospitalization unit. METHODS: This retrospective observational study included 828 patients admitted between January 2010 and February 2015 during the immediate postoperative period of bariatric surgery in a hospital. Data were collected via electronic medical records. The Mann-Whitney test was used to compare continuous variables, and the chi-square was used to compare categorical variables...
July 2017: Revista Brasileira de Terapia Intensiva
https://www.readbyqxmd.com/read/29043569/nutritional-support-and-parenteral-nutrition-in-cancer-patients-an-expert-consensus-report
#3
REVIEW
J A Virizuela, M Camblor-Álvarez, L M Luengo-Pérez, E Grande, J Álvarez-Hernández, M J Sendrós-Madroño, P Jiménez-Fonseca, M Cervera-Peris, M J Ocón-Bretón
BACKGROUND: Malnutrition is a frequent medical problem of cancer patients that negatively impacts their quality of life. METHODS: A multidisciplinary group of experts in Medical Oncology, Pharmacy, and Nutrition convened to discuss the management of the nutritional support in cancer patients. RESULTS: Of the 18 questions addressed, 9 focused on nutritional support, 5 were related to parenteral nutrition (PN) and 4 about home PN (HPN). The panel of experts recommends using nutritional screening routinely, at diagnosis and throughout the disease course, for detecting the risk of malnutrition and, if it is positive, to perform a complete nutritional assessment, to diagnose malnutrition...
October 17, 2017: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/29041830/general-outcomes-and-risk-factors-for-minor-and-major-amputations-in-brazil
#4
Jose O Leite, Leandro O Costa, Walter M Fonseca, Debora U Souza, Barbara C Goncalves, Gabriela B Gomes, Lucas A Cruz, Nilder Nister, Tulio P Navarro, Jonathan Bath, Alan Dardik
Objectives Major and minor amputations are associated with significant rates of mortality. However, little is known about the impact of unplanned redo-amputation during the same hospitalization on outcomes. The objectives of this study were to identify the risk factors associated with in-hospital mortality after both major and minor amputations as well as the results of unplanned redo-amputation on outcome. Methods Retrospective study of 342 consecutive patients who were treated with lower extremity amputation in Brazil between January 2013 and October 2014...
January 1, 2017: Vascular
https://www.readbyqxmd.com/read/29040447/transient-complete-atrioventricular-block-during-catheter-balloon-cryoablation-of-atrial-fibrillation-a-case-report
#5
Wallyson Pereira Fonseca, Cristiano F Pisani, Sissy Lara, Mauricio Scanavacca
No abstract text is available yet for this article.
October 12, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/29040222/neurovascular-response-during-exercise-and-mental-stress-in-anabolic-steroid-users
#6
Rafael Armani Porello, Marcelo Rodrigues Dos Santos, Francis Ribeiro de Souza, Guilherme Wesley Peixoto da Fonseca, Ana Luiza Carrari Sayegh, Tiago Franco de Oliveira, César Abreu Akiho, Maurício Yonamine, Rosa Maria Rodrigues Pereira, Carlos Eduardo Negrão, Maria-Janieire de Nazaré Nunes Alves
PURPOSE: Increased resting muscle sympathetic nerve activity (MSNA) and lower forearm blood flow (FBF) were observed in young men who use anabolic androgenic steroids (AAS). However, the response of MSNA and FBF in AAS users triggered by muscle mechanoreflex and central command has never been tested. Additionally, we evaluated the blood pressure (BP) and heart rate (HR) responses during these maneuvers. METHODS: Nineteen AAS users (AASU) aged 31±6 yr and 18 AAS nonusers (AASNU) aged 29±4 yr were recruited...
October 16, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29038767/evaluation-of-plasma-cytokines-in-patients-with-cocaine-use-disorders-in-abstinence-identifies-transforming-growth-factor-alpha-tgf%C3%AE-as-a-potential-biomarker-of-consumption-and-dual-diagnosis
#7
Rosa Maza-Quiroga, Nuria García-Marchena, Pablo Romero-Sanchiz, Vicente Barrios, María Pedraz, Antonia Serrano, Raquel Nogueira-Arjona, Juan Jesus Ruiz, Maribel Soria, Rafael Campos, Julie Ann Chowen, Jesus Argente, Marta Torrens, Meritxell López-Gallardo, Eva María Marco, Fernando Rodríguez de Fonseca, Francisco Javier Pavón, Pedro Araos
BACKGROUND: Cocaine use disorder (CUD) is a complex health condition, especially when it is accompanied by comorbid psychiatric disorders (dual diagnosis). Dual diagnosis is associated with difficulties in the stratification and treatment of patients. One of the major challenges in clinical practice of addiction psychiatry is the lack of objective biological markers that indicate the degree of consumption, severity of addiction, level of toxicity and response to treatment in patients with CUD...
2017: PeerJ
https://www.readbyqxmd.com/read/29037337/-social-cognition-and-its-contribution-to-the-rehabilitation-of-behavioural-disorders-in-traumatic-brain-injury
#8
José Ignacio Quemada, Olga Rusu, Paola Fonseca
Social behaviour disorders in traumatic brain injury are caused by the dysfunction of cognitive processes involved in social and interpersonal interaction. The concept of social cognition was introduced by authors studying schizophrenia, autism or mental retardation. The boundaries and the content of the concept have not yet been definitively defined, but theory of mind, empathy and emotional processing are included in all the models proposed. The strategies proposed to improve social behaviour focus on the restoration of cognitive processes such as working memory, emotional recognition and processing, and empathy, as well as social skills...
October 2017: Revista Colombiana de Psiquiatría
https://www.readbyqxmd.com/read/29033962/unveiling-chloroplast-rna-editing-events-using-next-generation-small-rna-sequencing-data
#9
Nureyev F Rodrigues, Ana P Christoff, Guilherme C da Fonseca, Franceli R Kulcheski, Rogerio Margis
Organellar RNA editing involves the modification of nucleotide sequences to maintain conserved protein functions, mainly by reverting non-neutral codon mutations. The loss of plastid editing events, resulting from mutations in RNA editing factors or through stress interference, leads to developmental, physiological and photosynthetic alterations. Recently, next generation sequencing technology has generated the massive discovery of sRNA sequences and expanded the number of sRNA data. Here, we present a method to screen chloroplast RNA editing using public sRNA libraries from Arabidopsis, soybean and rice...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29033934/ebi3-prevents-trypanosoma-cruzi-induced-myocarditis-by-dampening-ifn-%C3%AE-driven-inflammation
#10
Tiago Silva Medina, Gabriela Gonçalves Oliveira, Maria Cláudia Silva, Bruna Araújo David, Grace Kelly Silva, Denise Morais Fonseca, Renata Sesti-Costa, Amanda Farage Frade, Monique Andrade Baron, Barbara Ianni, Alexandre Costa Pereira, Christophe Chevillard, Edécio Cunha-Neto, José Antonio Marin-Neto, João Santana Silva
The identification of anti-inflammatory mediators can reveal important targetable molecules capable of counterbalancing Trypanosoma cruzi-induced myocarditis. Composed of Ebi3 and IL-27p28 subunits, IL-27 is produced by myeloid cells and is able to suppress inflammation by inducing IL-10-producing Tr1 cells, thus emerging as a potential candidate to ameliorate cardiac inflammation induced by T. cruzi. Although IL-27 has been extensively characterized as a suppressive cytokine that prevents liver immunopathogenesis after T...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29032814/healthy-dental-pulp-oxygen-saturation-rates-in-subjects-with-homozygous-sickle-cell-anemia-a-cross-sectional-study-nested-in-a-cohort
#11
Soraia de Fátima Carvalho Souza, Erika Bárbara Abreu Fonseca Thomaz, Cyrene Piazera Silva Costa
INTRODUCTION: To compare the percentage of arterial oxygen saturation (SpO2) in healthy teeth with confirmed pulp vitality between individuals with sickle cell anemia (HbSS) and normal hemoglobin A (HbAA). METHODS: This is a cross-sectional study nested within a cohort. Samples (n = 2543) comprised teeth with intact crowns and pulp vitality confirmed by thermal sensitivity tests and no history of caries, periodontal disease, or dental trauma. A total of 728 teeth of 113 individuals with HbSS and 1815 teeth of 246 individuals with HbAA were evaluated...
October 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29031924/evaluation-of-cervical-mucus-and-reproductive-efficiency-of-seasonally-anovular-dairy-goats-after-short-term-progestagen-based-estrous-induction-protocols-with-different-gonadotropins
#12
Jeferson F Fonseca, Joanna M G Souza-Fabjan, Maria Emilia F Oliveira, Renata C Cruz, Luciana V Esteves, Maria Pia S L Matos de Paiva, Felipe Z Brandão, Antônio B Mancio
The use of three different gonadotropins was tested for estrous induction in dairy goats during the non-breeding season. All does received an injection of 30 μg of d-cloprostenol and intravaginal sponges containing 60mg of medroxyprogesterone acetate (MAP) for 6 d plus 20 IU of porcine FSH (pFSH), 200 IU of eCG or 250 IU of hCG 24h before sponge removal. In Experiment 1 (n=24), ovarian ultrasound parameters were recorded and cervical mucus was evaluated daily for 5 d after sponge removal or until ovulation...
October 11, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29029922/submitting-tangential-nail-matrix-specimens
#13
Jorge Ocampo-Garza, Nilton Gioia Di Chiacchio, Judith Dominguez-Cherit, Leandro Fonseca Noriega, Nilton Di Chiacchio
No abstract text is available yet for this article.
November 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/29028629/a-dual-energy-micro-ct-methodology-for-visualization-and-quantification-of-biofilm-formation-and-dentin-demineralization
#14
Paula Maciel Pires, Thais Pires Dos Santos, Andrea Fonseca-Gonçalves, Matheus Melo Pithon, Ricardo Tadeu Lopes, Aline de Almeida Neves
OBJECTIVE: The aim of this study was to induce artificial caries in human sound dentin by means of a microcosm model using human saliva as source of bacteria and to apply a novel dual-energy micro-CT technique to quantify biofilm formation and evaluate its demineralization potential. DESIGN: Eight sound third molars had the occlusal enamel removed by cutting with a diamond disk and five cylindrical cavities (±2mm diameter; ±1.5mm depth) were prepared over the dentin surface in each specimen (n=40 cavities)...
October 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29026540/energetic-protein-supplementation-in-the-last-60%C3%A2-days-of-gestation-improves-performance-of-beef-cows-grazing-tropical-pastures
#15
Aline Gomes da Silva, Mário Fonseca Paulino, Edenio Detmann, Henrique Jorge Fernandes, Lincoln da Silva Amorim, Román Enrique Maza Ortega, Victor Valério de Carvalho, Josilaine Aparecida da Costa Lima, Felipe Henrique de Moura, Mariana Benevides Monteiro, Jéssika Almeida Bitencourt
BACKGROUND: Nutrition is one of the most important factors that affect animal performance, and it therefore also impacts on financial results in beef systems. In this way, finding the best strategy for feeding supplements is of paramount importance. Aiming to evaluate the effect of supplement feeding strategies for beef cows in the last third of gestation, two experiments were conducted. In Experiment 1, 35 pregnant Nellore cows were assigned to a completely randomized design with four treatments: control, which received no supplement; supplementation for the last 30 d of gestation (30-d; 3...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29025532/conflict-of-interest-disclosure-in-a-top-tier-portuguese-medical-journal
#16
Nuno Moreira Fonseca
INTRODUCTION: Scientific medical publications are considered to be a source of unbiased and independent information. Authors are required to disclose relationships with the pharmaceutical industry for transparency purposes. The aim of this work was to assess conflict of interest disclosure in a Portuguese top-tier medical journal by comparing authors' self-reported conflicts of interest with payments listed in the official database of Portuguese Ministry of Health. MATERIAL AND METHODS: All articles published in the Portuguese Journal of Cardiology from December 2015 to May 2016 were reviewed...
September 29, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/29019744/pediatric-liver-transplant-techniques-and-complications
#17
Natally Horvat, Antonio Sergio Zafred Marcelino, Joao Vicente Horvat, Tássia Regina Yamanari, Jose de Arimateia Batista Araújo-Filho, Pedro Panizza, Joao Seda-Neto, Eduardo Antunes da Fonseca, Francisco Cesar Carnevale, Luciana Mendes de Oliveira Cerri, Paulo Chapchap, Giovanni Guido Cerri
Liver transplant is considered to be the last-resort treatment approach for pediatric patients with end-stage liver disease. Despite the remarkable advance in survival rates, liver transplant remains an intricate surgery with significant morbidity and mortality. Early diagnosis of complications is crucial for patient survival but is challenging given the lack of specificity in clinical presentation. Knowledge of the liver and vascular anatomy of the donor and the recipient or recipients before surgery is also important to avoid complications...
October 2017: Radiographics: a Review Publication of the Radiological Society of North America, Inc
https://www.readbyqxmd.com/read/29019550/salivary-stress-biomarkers-and-anxiety-symptoms-in-children-with-and-without-temporomandibular-disorders
#18
Fernanda Yukie Kobayashi, Maria Beatriz Duarte Gavião, Maria Carolina Salomé Marquezin, Fernando Luiz Affonso Fonseca, Ana Bheatriz Marangoni Montes, Taís de Souza Barbosa, Paula Midori Castelo
The etiology of temporomandibular disorders (TMD), which are considered as a heterogeneous group of psychophysiological disturbances, remains a controversial issue in clinical dentistry. This study aimed to evaluate whether the salivary alpha-amylase (sAA), cortisol levels, and anxiety symptoms differ between children with and without TMD. Initially, 316 young subjects were screened in public schools (nonreferred sample); 76 subjects aged 7-14 years were selected and comprised the TMD and control groups with 38 subjects each matched by sex, age, and the presence/absence of sleep bruxism...
September 28, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/29018811/high-frequency-eeg-variations-in-children-with-autism-spectrum-disorder-during-human-faces-visualization
#19
Celina A Reis Paula, Camille Reategui, Bruna Karen de Sousa Costa, Caio Queiroz da Fonseca, Luana da Silva, Edgard Morya, Fabricio Lima Brasil
Autism spectrum disorder (ASD) is a neuropsychiatric disorder characterized by the impairment in the social reciprocity, interaction/language, and behavior, with stereotypes and signs of sensory function deficits. Electroencephalography (EEG) is a well-established and noninvasive tool for neurophysiological characterization and monitoring of the brain electrical activity, able to identify abnormalities related to frequency range, connectivity, and lateralization of brain functions. This research aims to evidence quantitative differences in the frequency spectrum pattern between EEG signals of children with and without ASD during visualization of human faces in three different expressions: neutral, happy, and angry...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29017832/impact-of-rhinitis-on-work-productivity-a-systematic-review
#20
Olivier Vandenplas, Denis Vinnikov, Paul D Blanc, Ioana Agache, Claus Bachert, Michael Bewick, Lars-Olaf Cardell, Paul Cullinan, Pascal Demoly, Alexis Descatha, Joao Fonseca, Tari Haahtela, Peter W Hellings, Jacques Jamart, Juha Jantunen, Ömer Kalayci, David Price, Boleslaw Samolinski, Joaquin Sastre, Longxiu Tian, Antonio L Valero, Xinyi Zhang, Jean Bousquet
BACKGROUND: Allergic rhinitis (AR) is increasingly acknowledged as having a substantial socioeconomic impact associated with impaired work productivity, although available information remains fragmented. OBJECTIVE: This systematic review summarizes recently available information to provide a quantitative estimate of the burden of AR on work productivity including lost work time (ie, absenteeism) and reduced performance while working (ie, presenteeism). METHODS: A Medline search retrieved original studies from 2005 to 2015 pertaining to the impact of AR on work productivity...
October 7, 2017: Journal of Allergy and Clinical Immunology in Practice
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