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https://www.readbyqxmd.com/read/28207310/microbiological-quality-and-prevalence-of-%C3%AE-lactam-antibiotic-resistance-genes-in-oysters-crassostrea-rhizophorae
#1
Maria Aparecida da RessurreiÇão Brandão, Amanda Teixeira Sampaio Lopes, Maria Tereza da Silva Neta, Rhyan Barros Farias de Oliveira, Rachel Passos Rezende, George Rêgo Albuquerque, Verônica Dias Gonçalves, Dália Dos Prazeres Rodrigues, Guisla Boehs, Bianca Mendes Maciel
The microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA...
February 16, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28193415/changes-of-sodium-nitrate-nitrite-and-n-nitrosodiethylamine-during-in-vitro-human-digestion
#2
Hyeong Sang Kim, Sun Jin Hur
This study aimed to determine the changes in sodium nitrate, sodium nitrite, and N-nitrosodiethylamine (NDEA) during in vitro human digestion, and the effect of enterobacteria on the changes in these compounds. The concentrations of nitrate, nitrite, and NDEA were significantly reduced from 150, 150, and 1ppm to 42.8, 63.2, and 0.85ppm, respectively, during in vitro human digestion (p<0.05). The enterobacteria Escherichia coli and Lactobacillus casei reduced the amount of these compounds present during in vitro human digestion...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28174560/things-are-getting-hairy-enterobacteria-bacteriophage-vb_pcam_cbb
#3
Colin Buttimer, Hanne Hendrix, Hugo Oliveira, Aidan Casey, Horst Neve, Olivia McAuliffe, R Paul Ross, Colin Hill, Jean-Paul Noben, Jim O'Mahony, Rob Lavigne, Aidan Coffey
Enterobacteria phage vB_PcaM_CBB is a "jumbo" phage belonging to the family Myoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the genera Erwinia, Pectobacterium, and Cronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis of the virions, 29% of its predicted ORFs could be functionally assigned...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28158310/retrospective-and-prospective-evaluation-of-the-carbapenem-inactivation-method-for-the-detection-of-carbapenemase-producing-enterobacteriaceae
#4
Lauraine Gauthier, Remy A Bonnin, Laurent Dortet, Thierry Naas
BACKGROUND: There is an urgent need for accurate and rapid diagnostic tests to identify carbapenemase producing enterobacteria (CPE). Here, we have evaluated the Carbapenem Inactivation Method (CIM) test to detect CPEs from cultured colonies. METHODS: A total of 256 enterobacterial isolates were used to evaluate the performance of the CIM in comparison to Carba NP test and molecular detection used a reference method. Ninety three well-characterized isolates (including 29 non-CPE and 63 CPEs of worldwide origin) with decreased susceptibility to at least one carbapenem were used to (i) evaluate the efficacy of CIM test and (ii) to compare it to the Carba NP test...
2017: PloS One
https://www.readbyqxmd.com/read/28111451/effects-of-sodium-diacetate-on-the-fermentation-profile-chemical-composition-and-aerobic-stability-of-alfalfa-silage
#5
XianJun Yuan, AiYou Wen, Seare T Desta, Jian Wang, Tao Shao
Objective: The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa (Medicago sativa L.) silage. Methods: Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7 and 9 g/kg of fresh forage). After 60 days of the ensiling, the samples were collected to examine the fermentative quality, chemical composition and aerobic stability. Results: The application of SDA significantly (P<0...
December 26, 2016: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28104282/-incidence-of-bacteria-from-cultures-of-bile-and-gallbladder-wall-of-laparoscopic-cholecystectomy-patients-in-the-university-hospital-dr-jos%C3%A3-eleuterio-gonz%C3%A3-lez
#6
Rubén Cueto-Ramos, Marco Hernández-Guedea, Edelmiro Pérez-Rodríguez, Francisco Reyna-Sepúlveda, Gerardo Muñoz-Maldonado
BACKGROUND: Through experience it has been accepted that bile in normal conditions remains sterile. Bactibilia is a common finding in individuals at high risk or with complicated cholecystolithiasis, however few data prevails about the prevalence of bactibilia in patients operated on for uncomplicated laparoscopic cholecystectomy. There is s common usage of preoperative and postoperative antibiotics in the different patients without the existence of any actual bacteriologic and epidemiologic evidence...
January 16, 2017: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/28040185/fate-of-the-microbial-population-and-the-physico-chemical-parameters-of-sanganel-a-typical-blood-sausages-of-the-friuli-a-north-east-region-of-italy
#7
Lucilla Iacumin, Marisa Manzano, Simone Stella, Giuseppe Comi
In Friuli, a Northeastern region of Italy, a blood sausage called Sanganel is produced by farmers, butchers, shops, and factories. This sausage is made with pork meat, boiled blood, lard, spices, and salt. It is stored at 4 ± 2 °C and usually eaten fresh or boiled within 14 days of its manufacture. Little is known about its microbial populations and safety for consumption. The aim of this study is to characterise the microbial populations and the physico-chemical parameters of Sanganel to establish its quality and the safety of consuming it...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28024523/zeolite-food-supplementation-reduces-abundance-of-enterobacteria
#8
Tanka P Prasai, Kerry B Walsh, Surya P Bhattarai, David J Midmore, Thi T H Van, Robert J Moore, Dragana Stanley
According to the World Health Organisation, antibiotics are rapidly losing potency in every country of the world. Poultry are currently perceived as a major source of pathogens and antimicrobial resistance. There is an urgent need for new and natural ways to control pathogens in poultry and humans alike. Porous, cation rich, aluminosilicate minerals, zeolites can be used as a feed additive in poultry rations, demonstrating multiple productivity benefits. Next generation sequencing of the 16S rRNA marker gene was used to phylogenetically characterize the fecal microbiota and thus investigate the ability and dose dependency of zeolite in terms of anti-pathogenic effects...
January 2017: Microbiological Research
https://www.readbyqxmd.com/read/28011950/involvement-of-corticotropin-releasing-factor-and-corticotropin-releasing-factor-2-receptors-in-pathogenesis-of-ischemia-reperfusion-induced-enteritis-in-rats
#9
K Takeuchi, A Kumano, N Abe, T Kotani
We herein investigated, using a corticotropin-releasing factor (CRF) agonist and antagonists, whether CRF plays a role in the pathogenesis of ischemia/reperfusion-induced small intestinal lesions in rats. Under pentobarbital anesthesia, the superior mesenteric artery was clamped (ischemia) for 75 min, followed by reperfusion with removal of the clamp. After a 24-h reperfusion, the area of hemorrhagic lesions that developed in the small intestine was measured. Urocortin I (CRF receptor 1/2 agonist), astressin (CRF receptor 1/2 antagonist), NBI27914 (CRF receptor 1 antagonist), or astressin 2B (CRF receptor 2 antagonist) was administered i...
October 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/27988173/first-survey-on-antibiotic-resistance-markers-in-enterobacteriaceae-in-cochabamba-bolivia
#10
Paola M Saba Villarroel, Gabriel O Gutkind, José A Di Conza, Marcela A Radice
A molecular survey was conducted in Cochabamba, Bolivia, to characterize the mechanism involved in the resistance to clinically relevant antibiotics. Extended Spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) markers were investigated in a total of 101 oxyimino-cephalosporin-resistant enterobacteria recovered from different health centers during four months (2012-2013). CTX-M enzymes were detected in all isolates, being the CTX-M-1 group the most prevalent (88.1%). The presence of blaOXA-1 was detected in 76...
December 14, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27938415/triatomine-bugs-their-microbiota-and-trypanosoma-cruzi-asymmetric-responses-of-bacteria-to-an-infected-blood-meal
#11
Sebastián Díaz, Bianca Villavicencio, Nathália Correia, Jane Costa, Karen L Haag
BACKGROUND: Triatomine bugs (Hemiptera: Reduviidae) are vectors of the flagellate Trypanosoma cruzi, the causative agent of Chagas disease. The study of triatomine gut microbiota has gained relevance in the last years due to its possible role in vector competence and prospective use in control strategies. The objective of this study is to examine changes in the gut microbiota composition of triatomines in response to a T. cruzi-infected blood meal and identifying key factors determining those changes...
December 9, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27917692/once-daily-high-dose-tigecycline-pharmacokinetic-pharmacodynamic-based-dosing-for-optimal-clinical-effectiveness-dosing-matters-revisited
#12
Burke A Cunha, Jeffrey Baron, Cheston B Cunha
Tigecycline has emerged as first line therapy for serious systemic infections due to important pathogens (except P. aeruginosa and Proteus sp.), including multi-drug resistant (MDR) and Gram negative bacilli (GNB), including carbapenem resistant Enterobacteriae. Tigecycline has a 'low resistance potential,' is protective against C. difficile, and is often the only antibiotic effective against MDR GNB, e.g., Klebsiella sp. Areas covered: Standard dose tigecycline therapy has been used for intra-abdominal infections, complicated skin/skin structure infections (cSSSIs), and CAP...
December 22, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27915087/genetically-unrelated-multidrug-and-carbapenem-resistant-citrobacter-freundii-detected-in-outpatients-admitted-to-a-portuguese-hospital
#13
Cátia Santos, Elmano Ramalheira, Gabriela Da Silva, Sónia Mendo
OBJECTIVES: Non-clonal, carbapenem- and multidrug-resistant Citrobacter freundii isolates were collected from unrelated outpatients admitted to a Portuguese hospital emergency department. One patient lived in a nursing home and was never hospitalised, whereas the other patient was repeatedly hospitalised in this hospital. The aim of this study was to unveil the molecular mechanisms associated with the carbapenem resistance of these isolates and to assess its potential dissemination. METHODS: Isolate identification was performed by VITEK(®)2 and was confirmed by 16S rDNA sequencing...
November 15, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27904402/microbiological-quality-and-variability-of-natural-microbiota-in-croatian-cheese-maturing-in-lambskin-sacks
#14
Jadranka Frece, Marija Vrdoljak, Mija Filipčić, Marko Jelić, Iva Čanak, Željko Jakopović, Jelka Pleadin, Ivana Gobin, Tibela Landeka Dragičević, Ksenija Markov
As in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. To do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. Two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. Not a single sample had Salmonella and Listeria monocytogenes, but we did find Escherichia coli in sheep's milk and cheese, and yeast and mould in both types of milk and cheese...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27877165/different-flour-microbial-communities-drive-to-sourdoughs-characterized-by-diverse-bacterial-strains-and-free-amino-acid-profiles
#15
Giuseppe Celano, Maria De Angelis, Fabio Minervini, Marco Gobbetti
This work aimed to investigate whether different microbial assemblies in flour may influence the microbiological and biochemical characteristics of traditional sourdough. To reach this purpose, members of lactic acid bacteria, enterobacteria, and yeasts were isolated from durum wheat flour. Secondly, the isolated microorganisms (Pediococcus pentosaceus, Saccharomyces cerevisiae, Pantoea agglomerans, and Escherichia hermannii) were inoculated in doughs prepared with irradiated flour (gamma rays at 10 kGy), so that eight different microbial assemblies were obtained...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27869509/encapsulated-benzoic-acid-supplementation-in-broiler-diets-influences-gut-bacterial-composition-and-activity
#16
M S Yousaf, F Goodarzi Boroojeni, W Vahjen, K Männer, A Hafeez, H Ur-Rehman, S Keller, S Peris, J Zentek
1. The present study investigated the effects of encapsulated benzoic acid (BA) supplementation in broiler feed on performance and gastrointestinal microbiota. 2. Eighty broilers were randomly divided into two groups. Birds in the control group were fed on maize-soybean-based diets. Birds in the treatment group were provided the same diet supplemented with 2 g/kg BA encapsulated in a vegetable oil matrix. 3. At the end of the trial (d 35), pH, bacterial composition and metabolites were determined in the crop, jejunum, ileum and caecum...
January 18, 2017: British Poultry Science
https://www.readbyqxmd.com/read/27866452/efficacy-of-debridement-antibiotic-therapy-and-implant-retention-within-three-months-during-postoperative-instrumented-spine-infections
#17
Heidi Wille, Frédéric-Antoine Dauchy, Arnaud Desclaux, Hervé Dutronc, Marc-Olivier Vareil, Véronique Dubois, Jean-Marc Vital, Michel Dupon
BACKGROUND: Postoperative instrumented spine infection (PISI) is a severe complication of invasive spine procedures. METHODS: Retrospective study of patients treated for PISI between 1st January 2008 and 31st December 2012 in a French University Hospital. The objectives of this study were to describe the outcome of patients treated with debridement-irrigation, antibiotic therapy and implant retention (DAIR) within three months after the occurrence of PISI and to identify factors associated with relapse...
April 2017: Infectious Diseases
https://www.readbyqxmd.com/read/27833933/data-on-enterobacteria-activity-on-biofilm-formation-at-surface-mango-fruit-mangifera-indica-l-cv-ataulfo
#18
Juan A Ragazzo-Sánchez, Ramsés R González-Estrada, Martha A Santana-Martínez, María Del Carmen Wacher-Rodarte, Carlos A Eslava-Campos, Montserrat Calderón-Santoyo
Abiotic factors influenced the capacity of the strains to form biofilms. Classification of the adhesion type is related with the optical density measured on the biofilm formation of tested strains. The relationship between the biofilm formation in real values with theoretical values of the strains was used to determine the mechanism involved during mixed cultures.
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27827942/changes-in-composition-and-function-of-human-intestinal-microbiota-exposed-to-chlorpyrifos-in-oil-as-assessed-by-the-shime-%C3%A2-model
#19
Julie Reygner, Claire Joly Condette, Aurélia Bruneau, Stéphane Delanaud, Larbi Rhazi, Flore Depeint, Latifa Abdennebi-Najar, Veronique Bach, Camille Mayeur, Hafida Khorsi-Cauet
The presence of pesticide residues in food is a public health problem. Exposure to these substances in daily life could have serious effects on the intestine-the first organ to come into contact with food contaminants. The present study investigated the impact of a low dose (1 mg/day in oil) of the pesticide chlorpyrifos (CPF) on the community structure, diversity and metabolic response of the human gut microbiota using the SHIME(®) model (six reactors, representing the different parts of the gastrointestinal tract)...
November 4, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27819272/diversity-of-bacteriome-associated-with-phlebotomus-chinensis-diptera-psychodidae-sand-flies-in-two-wild-populations-from-china
#20
Kaili Li, Huiying Chen, Jinjin Jiang, Xiangyu Li, Jiannong Xu, Yajun Ma
Sand fly Phlebotomus chinensis is a primary vector of transmission of visceral leishmaniasis in China. The sand flies have adapted to various ecological niches in distinct ecosystems. Characterization of the microbial structure and function will greatly facilitate the understanding of the sand fly ecology, which would provide critical information for developing intervention strategy for sand fly control. In this study we compared the bacterial composition between two populations of Ph. chinensis from Henan and Sichuan, China...
November 7, 2016: Scientific Reports
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