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https://www.readbyqxmd.com/read/27904402/microbiological-quality-and-variability-of-natural-microbiota-in-croatian-cheese-maturing-in-lambskin-sacks
#1
Jadranka Frece, Marija Vrdoljak, Mija Filipčić, Marko Jelić, Iva Čanak, Željko Jakopović, Jelka Pleadin, Ivana Gobin, Tibela Landeka Dragičević, Ksenija Markov
As in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. To do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. Two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. Not a single sample had Salmonella and Listeria monocytogenes, but we did find Escherichia coli in sheep's milk and cheese, and yeast and mould in both types of milk and cheese...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27877165/different-flour-microbial-communities-drive-to-sourdoughs-characterized-by-diverse-bacterial-strains-and-free-amino-acid-profiles
#2
Giuseppe Celano, Maria De Angelis, Fabio Minervini, Marco Gobbetti
This work aimed to investigate whether different microbial assemblies in flour may influence the microbiological and biochemical characteristics of traditional sourdough. To reach this purpose, members of lactic acid bacteria, enterobacteria, and yeasts were isolated from durum wheat flour. Secondly, the isolated microorganisms (Pediococcus pentosaceus, Saccharomyces cerevisiae, Pantoea agglomerans, and Escherichia hermannii) were inoculated in doughs prepared with irradiated flour (gamma rays at 10 kGy), so that eight different microbial assemblies were obtained...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27869509/encapsulated-benzoic-acid-supplementation-in-broiler-diets-influences-gut-bacterial-composition-and-activity
#3
M S Yousaf, F Goodarzi Boroojeni, W Vahjen, K Männer, A Hafeez, H Ur-Rehman, S Keller, S Peris, J Zentek
1. The present study investigated the effects of encapsulated benzoic acid (BA) supplementation in broiler feed on performance and gastrointestinal microbiota. 2. Eighty broilers were randomly divided into two groups. Birds in the control group were fed on maize-soybean based diets. Birds in the treatment group were provided the same diet supplemented with 2 g/kg BA encapsulated in a vegetable oil matrix. 3. At the end of the trial (d 35), pH, bacterial composition and metabolites were determined in the crop, jejunum, ileum and caecum...
November 21, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27866452/efficacy-of-debridement-antibiotic-therapy-and-implant-retention-within-three-months-during-postoperative-instrumented-spine-infections
#4
Heidi Wille, Frédéric-Antoine Dauchy, Arnaud Desclaux, Hervé Dutronc, Marc-Olivier Vareil, Véronique Dubois, Jean-Marc Vital, Michel Dupon
BACKGROUND: Postoperative instrumented spine infection (PISI) is a severe complication of invasive spine procedures. METHODS: Retrospective study of patients treated for PISI between 1st January 2008 and 31st December 2012 in a French University Hospital. The objectives of this study were to describe the outcome of patients treated with debridement-irrigation, antibiotic therapy and implant retention (DAIR) within three months after the occurrence of PISI and to identify factors associated with relapse...
November 21, 2016: Infectious Diseases
https://www.readbyqxmd.com/read/27833933/data-on-enterobacteria-activity-on-biofilm-formation-at-surface-mango-fruit-mangifera-indica-l-cv-ataulfo
#5
Juan A Ragazzo-Sánchez, Ramsés R González-Estrada, Martha A Santana-Martínez, María Del Carmen Wacher-Rodarte, Carlos A Eslava-Campos, Montserrat Calderón-Santoyo
Abiotic factors influenced the capacity of the strains to form biofilms. Classification of the adhesion type is related with the optical density measured on the biofilm formation of tested strains. The relationship between the biofilm formation in real values with theoretical values of the strains was used to determine the mechanism involved during mixed cultures.
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27827942/changes-in-composition-and-function-of-human-intestinal-microbiota-exposed-to-chlorpyrifos-in-oil-as-assessed-by-the-shime-%C3%A2-model
#6
Julie Reygner, Claire Joly Condette, Aurélia Bruneau, Stéphane Delanaud, Larbi Rhazi, Flore Depeint, Latifa Abdennebi-Najar, Veronique Bach, Camille Mayeur, Hafida Khorsi-Cauet
The presence of pesticide residues in food is a public health problem. Exposure to these substances in daily life could have serious effects on the intestine-the first organ to come into contact with food contaminants. The present study investigated the impact of a low dose (1 mg/day in oil) of the pesticide chlorpyrifos (CPF) on the community structure, diversity and metabolic response of the human gut microbiota using the SHIME(®) model (six reactors, representing the different parts of the gastrointestinal tract)...
November 4, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27819272/diversity-of-bacteriome-associated-with-phlebotomus-chinensis-diptera-psychodidae-sand-flies-in-two-wild-populations-from-china
#7
Kaili Li, Huiying Chen, Jinjin Jiang, Xiangyu Li, Jiannong Xu, Yajun Ma
Sand fly Phlebotomus chinensis is a primary vector of transmission of visceral leishmaniasis in China. The sand flies have adapted to various ecological niches in distinct ecosystems. Characterization of the microbial structure and function will greatly facilitate the understanding of the sand fly ecology, which would provide critical information for developing intervention strategy for sand fly control. In this study we compared the bacterial composition between two populations of Ph. chinensis from Henan and Sichuan, China...
November 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27818092/the-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-identification-versus-biochemical-tests-a-study-with-enterobacteria-from-a-dairy-cattle-environment
#8
Naiara Miranda Bento Rodrigues, Greiciane França Bronzato, Gabrielli Stefaninni Santiago, Larissa Alvarenga Batista Botelho, Beatriz Meurer Moreira, Irene da Silva Coelho, Miliane Moreira Soares de Souza, Shana de Mattos de Oliveira Coelho
Mastitis adversely affects milk production and in general cows do not regain their full production levels post recovery, leading to considerable economic losses. Moreover the percentage decrease in milk production depends on the specific pathogen that caused the infection and enterobacteria are responsible for this greater reduction. Phenotypic tests are among the currently available methods used worldwide to identify enterobacteria; however they tend to misdiagnose the species despite the multiple tests carried out...
September 21, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27806910/structure-and-function-of-pspa-and-vipp1-n-terminal-peptides-insights-into-the-membrane-stress-sensing-and-mitigation
#9
Christopher McDonald, Goran Jovanovic, B A Wallace, Oscar Ces, Martin Buck
The phage shock protein (Psp) response maintains integrity of the inner membrane (IM) in response to extracytoplasmic stress conditions and is widely distributed amongst enterobacteria. Its central component PspA, a member of the IM30 peripheral membrane protein family, acts as a major effector of the system through its direct association with the IM. Under non-stress conditions PspA also negatively regulates its own expression via direct interaction with the AAA+ ATPase PspF. PspA has a counterpart in cyanobacteria called Vipp1, which is implicated in protection of the thylakoid membranes...
October 30, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27798599/microcins-mediate-competition-among-enterobacteriaceae-in-the-inflamed-gut
#10
Martina Sassone-Corsi, Sean-Paul Nuccio, Henry Liu, Dulcemaria Hernandez, Christine T Vu, Amy A Takahashi, Robert A Edwards, Manuela Raffatellu
The Enterobacteriaceae are Gram-negative bacteria and include commensal organisms as well as primary and opportunistic pathogens that are among the leading causes of morbidity and mortality worldwide. Although Enterobacteriaceae often comprise less than 1% of a healthy intestine's microbiota(1), some of these organisms can bloom in the inflamed gut(2,3,4,5); indeed, expansion of enterobacteria is a hallmark of microbial imbalance known as "dysbiosis"(6). Microcins are small secreted proteins that possess antimicrobial activity in vitro(7,8), but whose role in vivo has been unclear...
October 31, 2016: Nature
https://www.readbyqxmd.com/read/27795309/esbl-pampc-producing-enterobacteria-in-the-broiler-hatchery-a-mode-of-pseudo-vertical-transmission
#11
Michaela Projahn, Katrin Daehre, Uwe Roesler, Anika Friese
: Antimicrobial resistance through extended-spectrum beta-lactamases (ESBLs) and transferable AmpC beta-lactamases represents an increasing problem in human and veterinary medicine. The presence of ESBL-/pAmpC-producing commensal enterobacteria in farm animals such as broiler chicken is considered as one possible source of food contamination and could, therefore, also be relevant for human colonization. Studies on transmission routes along the broiler production chain showed that already one-day old hatchlings are affected...
October 21, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27793826/exploring-the-impact-of-post-harvest-processing-on-the-microbiota-and-metabolite-profiles-during-a-case-of-green-coffee-bean-production
#12
Florac De Bruyn, Sophia Jiyuan Zhang, Vasileios Pothakos, Julio Torres, Charles Lambot, Alice V Moroni, Michael Callanan, Wilbert Sybesma, Stefan Weckx, Luc De Vuyst
: The post-harvest treatment and processing of fresh coffee cherries can impact the quality of the unroasted green coffee beans. In the present case study, freshly harvested Arabica coffee cherries were processed through two different wet and dry methods, to monitor differences in the microbial community structure, as well as substrate and metabolite profiles. The changes were followed throughout the entire post-harvest processing chain, from harvest to drying, by implementing up-to-date techniques, encompassing multiple-step metagenomic DNA extraction, high-throughput sequencing and multiphasic metabolite target analysis...
October 28, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27782071/effect-of-bifidobacterium-breve-on-the-intestinal-microbiota-of-coeliac-children-on-a-gluten-free-diet-a-pilot-study
#13
Andrea Quagliariello, Irene Aloisio, Nicole Bozzi Cionci, Donata Luiselli, Giuseppe D'Auria, Llúcia Martinez-Priego, David Pérez-Villarroya, Tomaž Langerholc, Maša Primec, Dušanka Mičetić-Turk, Diana Di Gioia
Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD)...
October 22, 2016: Nutrients
https://www.readbyqxmd.com/read/27770178/the-temperate-burkholderia-phage-ap3-of-the-peduovirinae-shows-efficient-antimicrobial-activity-against-b-cenocepacia-of-the-iiia-lineage
#14
Bartosz Roszniowski, Agnieszka Latka, Barbara Maciejewska, Dieter Vandenheuvel, Tomasz Olszak, Yves Briers, Giles S Holt, Miguel A Valvano, Rob Lavigne, Darren L Smith, Zuzanna Drulis-Kawa
Burkholderia phage AP3 (vB_BceM_AP3) is a temperate virus of the Myoviridae and the Peduovirinae subfamily (P2likevirus genus). This phage specifically infects multidrug-resistant clinical Burkholderia cenocepacia lineage IIIA strains commonly isolated from cystic fibrosis patients. AP3 exhibits high pairwise nucleotide identity (61.7 %) to Burkholderia phage KS5, specific to the same B. cenocepacia host, and has 46.7-49.5 % identity to phages infecting other species of Burkholderia. The lysis cassette of these related phages has a similar organization (putative antiholin, putative holin, endolysin, and spanins) and shows 29-98 % homology between specific lysis genes, in contrast to Enterobacteria phage P2, the hallmark phage of this genus...
October 21, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27728774/prevalence-of-plasmid-mediated-quinolone-resistance-genes-in-clinical-enterobacteria-from-argentina
#15
Ezequiel Albornoz, Celeste Lucero, Genara Romero, María Paula Quiroga, Melina Rapoport, Leonor Guerriero, Patricia Andres, Cecilia Rodriguez, Marcelo Galas, Daniela Centrón, Alejandra Corso, Alejandro Petroni
This first nationwide study was conducted to analyze the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in phenotypically unselected (consecutive) clinical enterobacteria. We studied 1,058 isolates that had been consecutively collected in 66 hospitals of the WHONET-Argentina Resistance Surveillance Network. Overall, 26% of isolates were nonsusceptible to at least one of the three quinolones tested (nalidixic acid, ciprofloxacin, and levofloxacin). The overall prevalence of PMQR genes was 8...
October 11, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27706388/-emergence-of-cmy-2-type-plasmid-mediated-ampc-%C3%AE-lactamase-in-shigella-sonnei-and-salmonella-spp-in-costa-rica-2003-2015
#16
Anamariela Tijerino Ayala, Hilda María Bolaños Acuña, María Teresa Acuña Calvo, José Luis Vargas Morales, Elena Campos Chacón
Plasmid-mediated AmpC are enzymes belonging to the group of β-lactamases and encoded by bla AmpC genes. Of these enzymes, those known as type CMY-2 are the most frequently reported worldwide. Detection of enterobacteria that produce CMY-2-type plasmid-mediated AmpC is clinically important since the use of β-lactam antibiotics can result in treatment failure. It is also important from a public health standpoint owing to the capacity for conjugative plasmid transfer to other enterobacteria, both within the community and in nosocomial environments...
August 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/27693407/the-pultra-plasmid-series-a-robust-and-flexible-tool-for-fluorescent-labeling-of-enterobacteria
#17
Despoina A I Mavridou, Diego Gonzalez, Abigail Clements, Kevin R Foster
Fluorescent labeling has been an invaluable tool for the study of living organisms and bacterial species are no exception to this. Here we present and characterize the pUltra plasmids which express constitutively a fluorescent protein gene (GFP, RFP, YFP or CFP) from a strong synthetic promoter and are suitable for the fluorescent labeling of a broad range of Enterobacteria. The amount of expressed fluorophore from these genetic constructs is such, that the contours of the cells can be delineated on the basis of the fluorescent signal only...
September 29, 2016: Plasmid
https://www.readbyqxmd.com/read/27672159/hypothesis-type-i-toxin-antitoxin-genes-enter-the-persistence-field-a-feedback-mechanism-explaining-membrane-homoeostasis
#18
REVIEW
Kenn Gerdes
Bacteria form persisters, cells that are tolerant to multiple antibiotics and other types of environmental stress. Persister formation can be induced either stochastically in single cells of a growing bacterial ensemble, or by environmental stresses, such as nutrient starvation, in a subpopulation of cells. In many cases, the molecular mechanisms underlying persistence are still unknown. However, there is growing evidence that, in enterobacteria, both stochastically and environmentally induced persistence are controlled by the second messenger (p)ppGpp...
November 5, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/27650830/emerging-resistant-bacteria-strains-in-bloodstream-infections-of-acute-leukaemia-patients-results-of-a-prospective-study-by-the-rete-ematologica-lombarda-rel
#19
Chiara Cattaneo, P Zappasodi, V Mancini, C Annaloro, F Pavesi, C Skert, A Ferrario, E Todisco, V Saccà, L Verga, A Passi, M Da Vià, S Ferrari, G Mometto, M Petullà, A Nosari, G Rossi
Multiresistant bacterial infections are a potentially life-threatening condition in acute leukaemia (AL) patients. We aimed to better define the very recent epidemiology and outcome of bloodstream infections (BSIs) in a real-life setting. We prospectively collected all consecutive febrile/infectious episodes occurring in AL patients admitted to 9 haematology units. In 293 AL patients, 433 BSIs were diagnosed. Gram-positive (GP) bacteria were isolated in 44.8 % BSI and Gram-negative (GN) in 38.3 %, while polymicrobial aetiology- or fungi-related events were identified in 15...
September 21, 2016: Annals of Hematology
https://www.readbyqxmd.com/read/27637802/qualitatively-and-quantitatively-investigating-the-regulation-of-intestinal-microbiota-on-the-metabolism-of-panax-notoginseng-saponins
#20
Jingcheng Xiao, Huimin Chen, Dian Kang, Yuhao Shao, Boyu Shen, Xinuo Li, Xiaoxi Yin, Zhangpei Zhu, Haofeng Li, Tai Rao, Lin Xie, Guangji Wang, Yan Liang
ETHNOPHARMACOLOGICAL RELEVANCE: Intestinal microflora plays crucial roles in modulating pharmacokinetic characteristics and pharmacological actions of active ingredients in traditional Chinese medicines (TCMs). However, the exact impact of altered intestinal microflora affecting the biotransformation of TCMs remains poorly understood. AIMS OF THE STUDY: This study aimed to reveal the specific enterobacteria which dominate the metabolism of panax notoginseng saponins (PNSs) via exploring the relationship between bacterial community structures and the metabolic profiles of PNSs...
September 13, 2016: Journal of Ethnopharmacology
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