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https://www.readbyqxmd.com/read/29785663/enterobacteria-and-host-resistance-to-infection
#1
REVIEW
Eugene Kang, Alanna Crouse, Lucie Chevallier, Stéphanie M Pontier, Ashwag Alzahrani, Navoun Silué, François-Xavier Campbell-Valois, Xavier Montagutelli, Samantha Gruenheid, Danielle Malo
Enterobacteriaceae are a large family of Gram-negative, non-spore-forming bacteria. Although many species exist as part of the natural flora of animals including humans, some members are associated with both intestinal and extraintestinal diseases. In this review, we focus on members of this family that have important roles in human disease: Salmonella, Escherichia, Shigella, and Yersinia, providing a brief overview of the disease caused by these bacteria, highlighting the contribution of animal models to our understanding of their pathogenesis and of host genetic determinants involved in susceptibility or resistance to infection...
May 21, 2018: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/29783046/bacterial-community-of-industrial-raw-sausage-packaged-in-modified-atmosphere-throughout-the-shelf-life
#2
Stefano Raimondi, Maria Rosaria Nappi, Tiziana Maria Sirangelo, Alan Leonardi, Alberto Amaretti, Alessandro Ulrici, Rudy Magnani, Chiara Montanari, Giulia Tabanelli, Fausto Gardini, Maddalena Rossi
Ten lots of industrial raw sausages in modified atmosphere (CO2 30%, O2 70%), produced in the same plant over 7 months, were analyzed at the day after production (S samples) and at the end of shelf life (E samples), after 12 days storage at 7 °C to simulate thermal abuse. Quality of the products was generally compromised by storage at 7 °C, with only 3 E samples without alterations. During the shelf life, the pH decreased for the accumulation of acetic and lactic acids. A few biogenic amines accumulated, remaining below acceptable limits...
April 28, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29782468/potential-role-of-gut-microbiota-in-als-pathogenesis-and-possible-novel-therapeutic-strategies
#3
Letizia Mazzini, Luca Mogna, Fabiola De Marchi, Angela Amoruso, Marco Pane, Irene Aloisio, Nicole Bozzi Cionci, Francesca Gaggìa, Ausiliatrice Lucenti, Enrica Bersano, Roberto Cantello, Diana Di Gioia, Giovanni Mogna
BACKGROUND: Recent preclinical studies suggest that dysfunction of gastrointestinal tract may play a role in amyotrophic lateral sclerosis (ALS) pathogenesis through a modification of the gut microbiota brain axis. Our study is the first focused on microbiota analysis in ALS patients. AIM: Our aim was to study the main human gut microbial groups and the overall microbial diversity in ALS and healthy subjects. Moreover we have examined the influence of a treatment with a specific bacteriotherapy composed of Lactobacillus strains (Lactobacillus fermentum, Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus salivarius) acting on the gastrointestinal barrier...
May 18, 2018: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/29781595/-results-of-the-implementation-of-an-antimicrobial-stewardship-program-in-the-gerencia-de-atenci%C3%A3-n-integrada-of-alcazar-de-san-juan-castilla-la-mancha
#4
M A Asencio Egea, O Herráez Carrera, M Huertas Vaquero, H D Patiño Ortega, M Franco Huerta, P Alcázar Carmona, M C Conde García, C Muñoz-Cuevas, C Román Ortiz, J Gaitán Pitera, R Carranza González, J R Barberá
OBJECTIVE: Our aim was to evaluate the efficiency of an ASP after its implementation in 2016 in a Spanish hospital quality system. METHODS: Efficiency of the ASP was measured by process and outcome indicators at the level of the patient's quality of life, antimicrobial consumption and percentage of resistance to them during the 2016-2017 period. In 2017, the failures mode and effects analysis (FMEA) methodology was applied. An annual satisfaction survey was conducted...
May 18, 2018: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29729918/the-effects-of-ultrasonication-fermentation-with-lactobacillus-sp-and-dehydration-on-the-chemical-composition-and-microbial-contamination-of-bovine-colostrum
#5
Elena Bartkiene, Vadims Bartkevics, Laura Elīna Ikkere, Iveta Pugajeva, Paulina Zavistanaviciute, Vita Lele, Modestas Ruzauskas, Jurga Bernatoniene, Valdas Jakstas, Dovile Klupsaite, Daiva Zadeike, Pranas Viskelis, Grazina Juodeikiene
The aim of this study was to evaluate the influence of ultrasonication, fermentation with Lactobacillus plantarum LUHS135 and Lactobacillus paracasei LUHS244, and different methods of dehydration on the chemical composition of bovine colostrum (BC), including the fatty acid and free amino acid profile and the content of micro- and macroelements. In addition, we analyzed the changes in lactic acid bacteria count, microbial contamination (aerobic mesophilic spore-forming bacteria, enterobacteria including Escherichia coli, and fungi/yeasts), the abundance of biogenic amines, and the concentration of nucleotide monophosphates...
May 2, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29718386/stpa-and-hha-stimulate-pausing-by-rna-polymerase-by-promoting-dna-dna-bridging-of-h-ns-filaments
#6
Beth A Boudreau, Daniel R Hron, Liang Qin, Ramon A van der Valk, Matthew V Kotlajich, Remus T Dame, Robert Landick
In enterobacteria, AT-rich horizontally acquired genes, including virulence genes, are silenced through the actions of at least three nucleoid-associated proteins (NAPs): H-NS, StpA and Hha. These proteins form gene-silencing nucleoprotein filaments through direct DNA binding by H-NS and StpA homodimers or heterodimers. Both linear and bridged filaments, in which NAPs bind one or two DNA segments, respectively, have been observed. Hha can interact with H-NS or StpA filaments, but itself lacks a DNA-binding domain...
April 30, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29713315/oral-administration-of-probiotics-increases-paneth-cells-and-intestinal-antimicrobial-activity
#7
Silvia I Cazorla, Carolina Maldonado-Galdeano, Ricardo Weill, Juan De Paula, Gabriela D V Perdigón
The huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. To meet this challenge, gut epithelial cells produce antimicrobial peptides (AMP) that act at the forefront of innate immunity. We explore whether this antimicrobial activity and Paneth cells, the main intestinal cell responsible of AMP production, are influenced by probiotics administration, to avoid the imbalance of intestinal microbiota and preserve intestinal barrier. Administration of Lactobacillus casei CRL 431 (Lc 431) and L...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29705860/bloodstream-infections-in-haematological-cancer-patients-colonized-by-multidrug-resistant-bacteria
#8
C Cattaneo, R Di Blasi, C Skert, A Candoni, B Martino, N Di Renzo, M Delia, S Ballanti, F Marchesi, V Mancini, E Orciuolo, S Cesaro, L Prezioso, R Fanci, G Nadali, A Chierichini, L Facchini, M Picardi, M Malagola, V Orlando, E M Trecarichi, M Tumbarello, F Aversa, G Rossi, L Pagano
Infections by multidrug-resistant (MDR) bacteria are a worrisome phenomenon in hematological patients. Data on the incidence of MDR colonization and related bloodstream infections (BSIs) in haematological patients are scarce. A multicentric prospective observational study was planned in 18 haematological institutions during a 6-month period. All patients showing MDR rectal colonization as well as occurrence of BSI at admission were recorded. One-hundred forty-four patients with MDR colonization were observed (6...
April 28, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29698660/enterobacteria-associated-with-houseflies-musca-domestica-as-an-infection-risk-indicator-in-swine-production-farms
#9
V Cervelin, G Fongaro, J B Pastore, F Engel, M A Reimers, A Viancelli
Houseflies (Musca domestica) spend part of their life development on animal or human manure. Manure is high in pathogenic microbes; thus, houseflies have been known as a mechanical vector for various important zoonotic diseases. Therefore, the present study showcases captured houseflies from intensive swine production regions (which are areas of high manure concentration) in Southern Brazil, and analyzes their bodies' to the presence of Escherichia coli and Salmonella sp. and the sensitivity of these bacteria to various antibiotics...
April 23, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29685282/silage-review-foodborne-pathogens-in-silage-and-their-mitigation-by-silage-additives
#10
REVIEW
O C M Queiroz, I M Ogunade, Z Weinberg, A T Adesogan
Silage is one of the main ingredients in dairy cattle diets and it is an important source of nutrients, particularly energy and digestible fiber. Unlike properly made and managed silage, poorly made or contaminated silage can also be a source of pathogenic bacteria that may decrease dairy cow performance, reduce the safety and quality dairy products, and compromise animal and human health. Some of the pathogenic bacteria that are frequently or occasionally associated with silage are enterobacteria, Listeria, Bacillus spp...
May 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29683323/a-trojan-horse-strategy-including-a-bacterial-suicide-action-for-the-efficient-use-of-a-specific-gram-positive-antibiotic-on-gram-negative-bacteria
#11
Isabelle J Schalk
In the alarming context of rising bacterial antibiotic resistance, there is an urgent need to discover new antibiotics or increase and/or enlarge the activity of those currently in use. The need for new antibiotics is even more urgent in the case of Gram-negative bacteria, such as Acinetobacter, Pseudomonas, and Enterobacteria, which have become resistant to many antibiotics and have an outer membrane with very low permeability to drugs. Vectorization of antibiotics using siderophores may be a solution to bypass such a bacterial wall: the drugs use the iron transporters of the outer membrane as gates to enter bacteria in a Trojan-horse strategy...
April 23, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29670725/association-of-liver-enzyme-levels-and-alveolar-bone-loss-a-cross-sectional-clinical-study-in-sado-island
#12
Ayumi Kuroki, Noriko Sugita, Shigeki Komatsu, Akio Yokoseki, Akihiro Yoshihara, Tetsuo Kobayashi, Kazutoshi Nakamura, Takeshi Momotsu, Naoto Endo, Kenji Sato, Ichiei Narita, Hiromasa Yoshie
Background: The interaction of periodontopathic bacteria with host immune system induces the production of inflammatory mediators which leads to alveolar bone loss (ABL), the essential feature of periodontitis. Concurrently, periodontal diseases cause the elevation of blood cytokine levels, the alteration of gut microbiota and the dissemination of enterobacteria to the liver. Owing to these mechanisms, periodontal disease might be a risk for liver dysfunction. Several epidemiological studies have reported associations between periodontal diseases and liver dysfunction, although the association between ABL and liver dysfunction has not been investigated...
February 2018: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/29652728/intestinal-microbiota-in-hirschsprung-disease
#13
Malla I Neuvonen, Katri Korpela, Kristiina Kyrklund, Risto J Rintala, Mikko P Pakarinen
OBJECTIVES: To characterize the microbiota profiles of patients with Hirschsprung's disease (HD), and to evaluate this in relation to postoperative bowel function and the incidence of Hirschsprung-associated enterocolitis (HAEC). METHODS: All patients operated on for HD at our center between 1987-2011 were invited to answer questionnaires on bowel function and to participate in a clinical follow-up for laboratory investigations, including fecal DNA extraction, fecal calprotectin (FC), and brush border lactase (LCT) genotyping...
April 12, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29642361/re-enterobacteria-secrete-an-inhibitor-of-pseudomonas-virulence-during-clinical-bacteriuria
#14
Anthony Atala
No abstract text is available yet for this article.
April 2018: Journal of Urology
https://www.readbyqxmd.com/read/29629146/establishment-of-a-universal-and-rational-gene-detection-strategy-through-three-way-junction-based-remote-transduction
#15
Yidan Tang, Baiyang Lu, Zhentong Zhu, Bingling Li
The polymerase chain reaction and many isothermal amplifications are able to achieve super gene amplification. Unfortunately, most commonly-used transduction methods, such as dye staining and Taqman-like probing, still suffer from shortcomings including false signals or difficult probe design, or are incompatible with multi-analysis. Here a universal and rational gene detection strategy has been established by translating isothermal amplicons to enzyme-free strand displacement circuits via three-way junction-based remote transduction...
January 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29587645/carrierseq-a-sequence-analysis-workflow-for-low-input-nanopore-sequencing
#16
Angel Mojarro, Julie Hachey, Gary Ruvkun, Maria T Zuber, Christopher E Carr
BACKGROUND: Long-read nanopore sequencing technology is of particular significance for taxonomic identification at or below the species level. For many environmental samples, the total extractable DNA is far below the current input requirements of nanopore sequencing, preventing "sample to sequence" metagenomics from low-biomass or recalcitrant samples. RESULTS: Here we address this problem by employing carrier sequencing, a method to sequence low-input DNA by preparing the target DNA with a genomic carrier to achieve ideal library preparation and sequencing stoichiometry without amplification...
March 27, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29568806/antibiotic-resistance-patterns-of-urinary-tract-pathogens-in-turkish-children
#17
Suzan Gunduz, Hatice Uludağ Altun
Background: Knowledge of local antimicrobial resistance patterns is essential for evidence- based empirical antibiotic prescribing. We aimed to investigate the distribution and changes in causative agents of urinary tract infections in children and the resistance rates, and to recommend the most appropriate antibiotics. Methods: In this retrospective study, we evaluated causative agents and antimicrobial resistance in urine isolates from the positive community from September 2014 to April 2016 in a single hospital in Ankara, Turkey...
2018: Global health research and policy
https://www.readbyqxmd.com/read/29559578/the-colibactin-genotoxin-generates-dna-interstrand-cross-links-in-infected-cells
#18
Nadège Bossuet-Greif, Julien Vignard, Frédéric Taieb, Gladys Mirey, Damien Dubois, Claude Petit, Eric Oswald, Jean-Philippe Nougayrède
Colibactins are hybrid polyketide-nonribosomal peptides produced by Escherichia coli , Klebsiella pneumoniae , and other Enterobacteriaceae harboring the pks genomic island. These genotoxic metabolites are produced by pks -encoded peptide-polyketide synthases as inactive prodrugs called precolibactins, which are then converted to colibactins by deacylation for DNA-damaging effects. Colibactins are bona fide virulence factors and are suspected of promoting colorectal carcinogenesis when produced by intestinal E...
March 20, 2018: MBio
https://www.readbyqxmd.com/read/29558430/effects-of-mutations-and-ligands-on-the-thermostability-of-the-l-arginine-agmatine-antiporter-adic-and-deduced-insights-into-ligand-binding-of-human-l-type-amino-acid-transporters
#19
Hüseyin Ilgü, Jean-Marc Jeckelmann, Claire Colas, Zöhre Ucurum, Avner Schlessinger, Dimitrios Fotiadis
The l-arginine/agmatine transporter AdiC is a prokaryotic member of the SLC7 family, which enables pathogenic enterobacteria to survive the extremely acidic gastric environment. Wild-type AdiC from Escherichia coli, as well as its previously reported point mutants N22A and S26A, were overexpressed homologously and purified to homogeneity. A size-exclusion chromatography-based thermostability assay was used to determine the melting temperatures ( T m s) of the purified AdiC variants in the absence and presence of the selected ligands l-arginine (Arg), agmatine, l-arginine methyl ester, and l-arginine amide...
March 20, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29556218/multiparametric-profiling-for-identification-of-chemosensitizers-against-gram-negative-bacteria
#20
Vincent Lôme, Jean-Michel Brunel, Jean-Marie Pagès, Jean-Michel Bolla
Antibiotic resistance is now a worldwide therapeutic problem. Since the beginning of anti-infectious treatment bacteria have rapidly shown an incredible ability to develop and transfer resistance mechanisms. In the last decades, the design variation of pioneer bioactive molecules has strongly improved their activity and the pharmaceutical companies partly won the race against the clock. Since the 1980s, the new classes of antibiotics that emerged were mainly directed to Gram-positive bacteria. Thus, we are now facing to multidrug-resistant Gram-negative bacteria, with no therapeutic options to deal with them...
2018: Frontiers in Microbiology
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