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https://www.readbyqxmd.com/read/28654984/osteoprotegerin-is-a-new-regulator-of-inflammation-and-angiogenesis-in-proliferative-diabetic-retinopathy
#1
Ahmed M Abu El-Asrar, Sofie Struyf, Ghulam Mohammad, Mieke Gouwy, Pieter Rytinx, Mohammad Mairaj Siddiquei, Cristina Hernández, Kaiser Alam, Ahmed Mousa, Gert De Hertogh, Ghislain Opdenakker, Rafael Simó
Purpose: Osteoprotegerin (OPG) is a novel regulator of endothelial cell function, angiogenesis, and vasculogenesis. We correlated expression levels of OPG with those of the angiogenic and inflammatory factors vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1/CCL2) in proliferative diabetic retinopathy (PDR). We also examined expression of OPG in retinas from diabetic rats and diabetic patients and measured production of OPG by human retinal microvascular endothelial cells (HRMEC) and investigated its angiogenic activity...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28654910/microrna16-regulates-glioma-cell-proliferation-apoptosis-and-invasion-by-targeting-wip1-atm-p53-feedback-loop
#2
Xiao-Hong Zhan, Qiu-Yan Xu, Rui Tian, Hong Yan, Min Zhang, Jing Wu, Wei Wang, Jie He
The present study aimed to investigate the role and underlying mechanisms of microRNA16 (miR-16) on proliferation, apoptosis and invasion of glioma cells. The cell models of miR-16 upregulation and Negative control group (NC group) were built. The cell functions of different groups were detected by colony formation assay, transwell chamber assay, proliferation, apoptosis and cycle experiments. The intracranial orthotopic transplantation animal models were built to different groups: miR-16 agomir group, miR-16 antagomir group and their NC group...
June 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28654884/specific-and-selective-probes-for-staphylococcus-aureus-from-phage-displayed-random-peptide-libraries
#3
Laura M De Plano, Santina Carnazza, Grazia M L Messina, Maria Giovanna Rizzo, Giovanni Marletta, Salvatore P P Guglielmino
Staphylococcus aureus is a major human pathogen causing health care-associated and community-associated infections. Early diagnosis is essential to prevent disease progression and to reduce complications that can be serious. In this study, we selected, from a 9-mer phage peptide library, a phage clone displaying peptide capable of specific binding to S. aureus cell surface, namely St.au9IVS5 (sequence peptide RVRSAPSSS).The ability of the isolated phage clone to interact specifically with S. aureus and the efficacy of its bacteria-binding properties were established by using enzyme linked immune-sorbent assay (ELISA)...
June 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28654864/wolf-hirschhorn-syndrome-candidate-1-whsc1-functions-as-a-tumor-suppressor-by-governing-cell-differentiation
#4
Chuan Yu, Xiaomin Yao, Linjie Zhao, Ping Wang, Qian Zhang, Chengjian Zhao, Shaohua Yao, Yuquan Wei
Wolf-Hirschhorn syndrome candidate 1 (WHSC1) is a histone 3 lysine 36 (H3K36) specific methyltransferase that is frequently deleted in Wolf-Hirschhorn syndrome (WHS). Whsc1 is also found mutated in a subgroup of B-cell derived malignant diseases by genomic translocation or point mutation, both of which resulted in hyperactivity of WHSC1 mediated H3K36 methylation and uncontrolled cell proliferation, suggesting that whsc1 functions as an oncogene. However, here we provided evidences to show that whsc1 also has tumor suppressor functions...
June 24, 2017: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/28654829/fructose-intake-exacerbates-the-contractile-response-elicited-by-norepinephrine-in-mesenteric-vascular-bed-of-rats-via-increased-endothelial-prostanoids
#5
Glauciene J Sousa, Phablo Wendell C Oliveira, Breno V Nogueira, Antônio F Melo, Thaís de Oliveira Faria, Eduardo Frizera Meira, José G Mill, Nazaré S Bissoli, Marcelo P Baldo
Chronic fructose intake induces major cardiovascular and metabolic disturbances and is associated with the development of hypertension due to changes in vascular function. We hypothesized that high fructose intake for 6 weeks would cause metabolic syndrome and lead to initial vascular dysfunction. Male Wistar rats were assigned to receive fructose (FRU, 10%) or drinking water (CON) for 6 weeks. Systolic blood pressure was evaluated by tail plethysmography. Fasting glucose, insulin and glucose tolerance were measured at the end of the follow-up...
June 20, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28654793/pure-total-flavonoids-from-citrus-improve-non-alcoholic-fatty-liver-disease-by-regulating-tlr-ccl-signaling-pathway-a-preliminary-high-throughput-omics-study
#6
Liyan Wu, Maoxiang Yan, Jianping Jiang, Beihui He, Wei Hong, Zhiyun Chen
OBJECTIVE: This study investigated the possible molecular mechanisms of pure total flavonoids from citrus (PTFC) for the treatment of non-alcoholic fatty liver disease (NAFLD) via toll-like receptor/C-C chemokine ligand (TLR/CCL) signaling pathways by monitoring the changes in gene expression profile in liver tissues induced by high-fat diet. METHODS: We performed systematical analyses on hepatic expression profiles of mRNAs in a high-fat diet (HFD)-induced steatotic animal model with or without PTFC treatment...
June 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28654764/deep-proteome-profiling-reveals-common-prevalence-of-mzb1-positive-plasma-b-cells-in-human-lung-and-skin-fibrosis
#7
Herbert B Schiller, Christoph H Mayr, Gabriela Leuschner, Maximilian Strunz, Claudia Staab-Weijnitz, Stefan Preisendörfer, Beate Eckes, Pia Moinzadeh, Thomas Krieg, David A Schwartz, Rudolf A Hatz, Jürgen Behr, Matthias Mann, Oliver Eickelberg
RATIONALE: Analyzing the molecular heterogeneity of different forms of organ fibrosis may reveal common and specific factors and thus identify potential future therapeutic targets. OBJECTIVES: We sought to use proteome-wide profiling of human tissue fibrosis to (1) identify common and specific signatures across endstage interstitial lung disease (ILD) cases, (2) characterize ILD subgroups in an unbiased fashion, and (3) identify common and specific features of lung and skin fibrosis...
June 27, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28654654/identification-and-characterization-of-acka-dependent-protein-acetylation-in-neisseria-gonorrhoeae
#8
Deborah M B Post, Birgit Schilling, Lorri M Reinders, Alexandria K D'Souza, Margaret R Ketterer, Steven J Kiel, Aroon T Chande, Michael A Apicella, Bradford W Gibson
Neisseria gonorrhoeae, the causative agent of gonorrhea, has a number of factors known to contribute to pathogenesis; however, a full understanding of these processes and their regulation has proven to be elusive. Post-translational modifications (PTMs) of bacterial proteins are now recognized as one mechanism of protein regulation. In the present study, Western blot analyses, with an anti-acetyl-lysine antibody, indicated that a large number of gonococcal proteins are post-translationally modified. Previous work has shown that Nε-lysine acetylation can occur non-enzymatically with acetyl-phosphate (AcP) as the acetyl donor...
2017: PloS One
https://www.readbyqxmd.com/read/28654632/p53-and-protein-phosphorylation-regulate-the-oncogenic-role-of-epithelial-cell-transforming-2-ect2
#9
Yan Chen, Ping Tian, Yi Liu
BACKGROUND Gastric cancer (GC) is the second leading cause of cancer-related death worldwide, but little progress has been achieved in the treatment of advanced or metastatic GC. GC is highly heterogeneous and more studies are needed to elucidate the metastatic mechanisms. Epithelial cell transforming 2 (ECT2) has been reported to be up-regulated in GC tissues, but its signaling mechanisms remain unclear. MATERIAL AND METHODS In this study, we used Western blot analysis to compare the expression level of ECT2 in 2 GC cell lines: MKN1 and MKN45...
June 27, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28654149/young-adult-survivors-of-childhood-acute-lymphoblastic-leukemia-show-evidence-of-chronic-inflammation-and-cellular-aging
#10
Hany Ariffin, Mohamad Shafiq Azanan, Sayyidatul Syahirah Abd Ghafar, Lixian Oh, Kee Hie Lau, Tharshanadhevasheri Thirunavakarasu, Atiqah Sedan, Kamariah Ibrahim, Adelyne Chan, Tong Foh Chin, Fong Fong Liew, Shareni Jeyamogan, Erda Syerena Rosli, Rashidah Baharudin, Tsiao Yi Yap, Roderick Skinner, Su Han Lum, Pierre Hainaut
BACKGROUND: Large epidemiologic studies have reported the premature onset of age-related conditions, such as ischemic heart disease and diabetes mellitus, in childhood cancer survivors, decades earlier than in their peers. The authors investigated whether young adult survivors of childhood acute lymphoblastic leukemia (ALL) have a biologic phenotype of cellular ageing and chronic inflammation. METHODS: Plasma inflammatory cytokines were measured using a cytometric bead array in 87 asymptomatic young adult survivors of childhood ALL (median age, 25 years; age range, 18-35 years) who attended annual follow-up clinic and compared with healthy, age-matched and sex-matched controls...
June 27, 2017: Cancer
https://www.readbyqxmd.com/read/28654057/murine-aortic-crush-injury-an-efficient-in-vivo-model-of-smooth-muscle-cell-proliferation-and-endothelial-function
#11
Dan Yu, George Makkar, Rajabrata Sarkar, Dudley K Strickland, Thomas S Monahan
Arterial reconstruction, whether angioplasty or bypass surgery, involves iatrogenic trauma causing endothelial disruption and vascular smooth muscle cell (VSMC) proliferation. Common murine models study small vessels such as the carotid and femoral arteries. Herein we describe an in vivo system in which both VSMC proliferation and endothelial barrier function can be simultaneously assessed in a large vessel. We studied the infrarenal aortic response to injury in C57BL/6 mice. The aorta was injured from the left renal vein to the aortic bifurcation by 30 transmural crushes of 5-seconds duration with a cotton-tipped applicator...
June 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28653898/toll-like-receptor-4-promotes-proliferation-and-apoptosis-resistance-in-human-papillomavirus-related-cervical-cancer-cells-through-the-toll-like-receptor-4-nuclear-factor-%C3%AE%C2%BAb-pathway
#12
Ninghong Jiang, Feng Xie, Qisang Guo, Ming-Qing Li, Jingjing Xiao, Long Sui
Toll-like receptor 4 is overexpressed in various tumors, including cervical carcinoma. However, the role of Toll-like receptor 4 in cervical cancer remains controversial, and the underlying mechanisms are largely elusive. Therefore, Toll-like receptor 4 in cervical cancer and related mechanisms were investigated in this study. Quantitative reverse transcription polymerase chain reaction and western blot analyses were used to detect messenger RNA and protein levels in HeLa, Caski, and C33A cells with different treatments...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28653890/expression-of-fra-1-in-human-hepatocellular-carcinoma-and-its-prognostic-significance
#13
Xiao-Qiang Gao, Yong-Sheng Ge, Qing-Hua Shu, Hua-Xing Ma
This study aimed to explore the clinical significance and prognostic value of Fra-1 in hepatocellular carcinoma patients after curative resection. Fra-1 expression was investigated using a combination of techniques: immunohistochemistry for 66 samples of hepatocellular carcinoma and quantitative real-time polymerase chain reaction and western blotting assays for 19 matched hepatocellular carcinoma specimens. Fra-1 was present in 38 of 66 (57.6%) tumor tissues, with intense staining in the nuclei. There was also positive staining in 14 of 66 (21...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28653878/sirtuin-6-plays-an-oncogenic-role-and-induces-cell-autophagy-in-esophageal-cancer-cells
#14
Nan Huang, Zhiwei Liu, Jiabei Zhu, Zhongqi Cui, Yuguang Li, Yongchun Yu, Fenyong Sun, Qiuhui Pan, Qingyuan Yang
Sirtuin 6, a member of sirtuin family, is generally regarded as a tumor suppressor as it participates in suppressing hypoxia-inducible factor 1α and MYC transcription activity by deacetylating H3K9 (histone H3 lysine 9) and H3K56 (histone H3 lysine) at promoters of target genes, leading to the aerobic glycolysis inhibition and cell growth suppression. However, its expression has recently been reported to be highly elevated in a series of tumors, including prostate cancer, breast cancer, and non-small cell lung cancer, indicating that sirtuin 6 plays dual roles in tumorigenicity in a cell/tumor type-specific manner...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28653874/invalidation-of-mitophagy-by-fbp1-mediated-repression-promotes-apoptosis-in-breast-cancer
#15
Yifeng Liu, Yulin Jiang, Nian Wang, Qianni Jin, Feihu Ji, Changli Zhong, Zhiqiang Zhang, Junhong Yang, Xiangsen Ye, Tingmei Chen
Fructose-1,6-bisphosphatase 1, a rate-limiting enzyme in gluconeogenesis, was recently shown to be a tumor suppressor. However, the functions of fructose-1,6-bisphosphatase 1 in the regulation of mitophagy and apoptosis remain unknown. Here, we investigated the effects of fructose-1,6-bisphosphatase 1 on mitophagy and apoptosis as well as their underlying mechanisms in breast cancer cells. In this work, the messenger RNA and protein expression of various molecules were determined by quantitative realtime polymerase chain reaction and western blot, respectively...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28653781/differential-expression-profile-of-long-non-coding-rna-in-cardiomyocytes-autophagy-induced-by-angiotensin-ii
#16
Ying Gu, Fan Yang, Ru-Ming Xu, Yun-Yan Zhang, Yang Li, Su-Xuan Liu, Guan-Xin Zhang, Guo-Kun Wang, Li-Ping Ma
Autophagy is a ubiquitous intracellular process for cellular homeostasis maintainance by recycling damaged protein and organelles. Dysregulation of cardiomyocytes autophagic activity is implicated in various heart diseases. Recent studies had demonstrated long non-coding RNAs (lncRNAs) played crucial roles on modulation of autophagic activity. In this study, we first established an angiotensin II-induced autophagy model on neonatal rat cardiomyocytes. Western blot assay confirmed that the expression of Beclin 1 and the conversion of soluble LC3-I to lipid bound LC3-II were significantly increased at 12h after angiotensin II stimulation, but the cardiomyocytes surface area and hypertrophic markers expression had no significant change...
June 27, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28653604/microrna-139-5p-inhibit-cell-proliferation-and-invasion-by-targeting-rho-associated-coiled-coil-containing-protein-kinase-2-in-ovarian-cancer
#17
Yanli Wang, Jia Li, Chunling Xu, Xiaomeng Zhang
Increasing evidence indicates that the dysregulation of microRNAs is associated with the development and progression ofvarious cancers. MicroRNA-139-5p (miR-139-5p) has been reported to have tumor-suppressive role in many types of cancers. But the role of miR-139-5p in ovarian cancer (OC) is poorly understood. The purpose of the present study was to explore the expression of miR-139-5p and its function in OC. The results showed that miR-139-5p expression was markedly downregulated in OC tissues and cell lines...
June 14, 2017: Oncology Research
https://www.readbyqxmd.com/read/28653599/microrna-539-inhibits-the-epithelial-mesenchymal-transition-of-esophageal-cancer-cells-by-twist-related-protein-1-mediated-modulation-of-melanoma-associated-antigen-a4-magea4
#18
Zhili Cao, Xiang Zheng, Lei Cao, Naixin Liang
MicroRNAs (miR) play key roles in cancers, yet the potential molecular mechanisms of miR-539 on esophageal squamous cell carcinoma (ESCC) are not well understood. Screened by informatics method, Twist-related protein 1 (TWIST1) was hypothesized to be a possible target gene of miR-539. Since the melanoma associated antigen (MAGE) A4 is reported to be up-regulated by TWIST1, this study aimed to examine the biological functions and mechanism involving TWIST1 and MAGE4 of miR-539 in ESCC. MiR-539 mimics or scrambled miRs were transfected into human ESCC TE3 cells to interfere the expression of miR-539...
June 12, 2017: Oncology Research
https://www.readbyqxmd.com/read/28653288/progesterone-suppresses-the-invasion-and-migration-of-breast-cancer-cells-irrespective-of-their-progesterone-receptor-status-a-short-report
#19
Mukul Godbole, Kanishka Tiwary, Rajendra Badwe, Sudeep Gupta, Amit Dutt
PURPOSE: Pre-operative progesterone treatment of breast cancer has been shown to confer survival benefits to patients independent of their progesterone receptor (PR) status. The underlying mechanism and the question whether such an effect can also be observed in PR negative breast cancer cells remain to be resolved. METHODS: We performed proteome profiling of PR-positive and PR-negative breast cancer cells in response to progesterone using a phospho-kinase array platform...
June 26, 2017: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/28653252/sil-24-peptide-a-human-interleukin-24-isoform-induces-mitochondrial-mediated-apoptosis-in-human-cancer-cells
#20
Samira Valiyari, Mona Salami, Reza Mahdian, Mohammad Ali Shokrgozar, Mana Oloomi, Azadeh Mohammadi Farsani, Saeid Bouzari
PURPOSE: Interleukin-24 (IL-24) is a unique cytokine in the IL-10 family that reveals tumor-suppressive activity against a broad range of cancers. Alternative splicing of human IL-24 generates several isoforms with different pro-apoptotic activities. In the current study, we aimed to investigate the cytotoxic properties of a recombinant smallest isoform of IL-24 (sIL-24) and the underlying molecular mechanisms in PC-3, A549, U937, and Raji cancer cells as well as normal cell line MRC-5...
June 26, 2017: Cancer Chemotherapy and Pharmacology
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