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https://www.readbyqxmd.com/read/28441859/-dnt-cell-inhibits-the-growth-of-pancreatic-carcinoma-via-nkg2d-and-mica-pathway-in-vivo
#1
J Chen, H Xu, X X Zhu, Y Zhao, H Z Fang, J Q Zhao
Objective: To investigate the effects of natural killer group 2 member D (NKG2D) and its ligands major histocompatibility complex class Ⅰ chain-related molecules A(MICA) on DNT cell killing pancreatic carcinoma. Methods: Antibodies adsorption was used to separate DNT cell from human peripheral blood. Human pancreatic tumor models were established via implanting BXPC-3 cells into nude mice, and then mice were randomly divided mice into 3 groups, blank group, gemcitabine group and DNT group. Mice weights and mice tumor volumes were measured every 5 days...
April 25, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28441794/reciprocal-interactions-of-leukemic-cells-with-bone-marrow-stromal-cells-promote-enrichment-of-leukemic-stell-cell-compartments-in-response-to-curcumin-and-daunorubicin
#2
Saeed Mohammadi, Mohsen Nikbakht, Seyed Mehdi Sajjadi, Fariba Rad, Bahram Chahardouli, Javid Sabour Takanlu, Shahrbano Rostami, Kamran Alimoghaddam, Ardeshir Ghavamzadeh, Seyed H Ghaffari
A predominant challenge in developing curative leukemia therapy is interactions of leukemic cells with the bone marrow stromal microenvironment. We aimed to investigate the role of stromal cells, such as bone marrow mesenchymal stromal cells (BMSCs) and osteoblasts (OBs), in curcumin (CUR) and daunorubicin (DNR) induced apoptosis of acute myeloid leukemia (AML) cells. We used KG1 and U937 as leukemia cell line models and treated them with CUR and DNR. The cells were then co-cultured with BMSCs or a combination of BMSCs and OBs as feeders...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28441774/brain-derived-neurotrophic-factor-mediated-perfluorooctane-sulfonate-induced-neurotoxicity-via-epigenetics-regulation-in-sk-n-sh-cells
#3
Xin-Xin Guo, Qing-Zhi He, Wu Li, Ding-Xin Long, Xiao-Yuan Pan, Cong Chen, Huai-Cai Zeng
Perfluorooctane sulfonate (PFOS), a new kind of persistent organic pollutant, is widely distributed in the environment and exists in various organisms, where it is also a neurotoxic compound. However, the potential mechanism of its neurotoxicity is still unclear. To examine the role of epigenetics in the neurotoxicity induced by PFOS, SK-N-SH cells were treated with different concentrations of PFOS or control medium (0.1% DMSO) for 48 h. The mRNA levels of DNA methyltransferases (DNMTs) and Brain-derived neurotrophic factor (BDNF), microRNA-16, microRNA-22, and microRNA-30a-5p were detected by Quantitative PCR (QPCR)...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28441717/dexmedetomidine-relieves-formaldehyde-induced-pain-in-rats-through-both-%C3%AE-2-adrenoceptor-and-imidazoline-receptor
#4
Hong Zhang, Xiang Yan, De-Gui Wang, Yu-Fang Leng, Zhan-Hai Wan, Yong-Qiang Liu, Yan Zhang
Dexmedetomidine (DEX) is a highly selective α2 adrenergic receptor agonist. In this study, we investigated the analgesic effect and the underlying mechanisms of DEX on inflammatory visceral pain in rats. Twenty-five male Sprague Dawley (SD) rats were randomly divided into 5 groups, including control, sham, low dose DEX, medium dose DEX and high dose DEX group. Pain was induced with 10% formalin and scored every 15min till 2 h-post the induction. Hematoxylin-eosin (HE) staining was used to evaluate the toxicity of DEX on spinal cord neurons...
April 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28441714/long-non-coding-rna-hotair-functions-as-mirna-sponge-to-promote-the-epithelial-to-mesenchymal-transition-in-esophageal-cancer
#5
Feng Xu, Jing Zhang
BACKGROUND: Accumulating evidence indicates dysregulated expression of the long non-coding RNA HOTAIR (lncRNA-HOTAIR) may play a significant role in tumor progression. LncRNA-HOTAIR promotes several processes in esophageal cancer (EC), including cell growth, differentiation, invasion and migration. However, the mechanisms by which lncRNA-HOTAIR promotes invasion and migration EC remain unclear. METHODS: LncRNA-HOTAIR and miR-148a expression were quantified in 40 paired human EC and tumor-adjacent tissues and EC cell lines by quantitative real-time PCR (qRT-PCR)...
April 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28441666/a-microrna-124-polymorphism-is-associated-with-fracture-healing-via-modulating-bmp6-expression
#6
Lin Zou, Guichun Zhang, Lifeng Liu, Chen Chen, Xuecheng Cao, Jinfang Cai
BACKGROUND: miR-124-3p has been reported to be involved in the pathogenesis of many diseases by modulating a variety of signaling pathways. In this study, we aimed to understand the impact of miR-124-3p expression level on the fracture healing in the patients of metaphyseal fracture of distal tibia, who received minimal invasive percutaneous plate osteosynthesis. METHODS: We firstly collected 195 patients of metaphyseal fracture of distal tibia, and the genotype of rs531564 was determined: GG (n=124) and GC+CC (n=71)...
April 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28441662/buffalo-leukemia-inhibitory-factor-induces-differentiation-and-dome-like-secondary-structures-in-cos-1-cells
#7
Gurjeet Kaur, Syed Azmal Ali, Shikha Pachauri, Dhruba Malakar, Jai K Kaushik, Ashok K Mohanty, Sudarshan Kumar
This study aimed to understand the molecular characteristics of buffalo leukemia inhibitory factor (BuLIF) and the generation of a stably transfected COS-1_BuLIF cell line for its functional characterization. Cumulus cells, isolated from oocytes, were separated, and total cDNA was prepared. The BuLIF gene was ligated into the cloning vector pJET1.2/blunt and expression vector pAcGFP-N1 which was transfected into COS-1 cells and confirmed by qRT-PCR and Western blot. BuLIF was immunoprecipitated and evaluated through a MTT assay...
April 26, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28441631/up-regulated-baff-and-baff-receptor-expression-in-patients-with-intractable-temporal-lobe-epilepsy-and-a-pilocarpine-induced-epilepsy-rat-model
#8
Limin Ma, Ruohan Li, Hao Huang, Jinxian Yuan, Shu Ou, Tao Xu, Xinyuan Yu, Xi Liu, Yangmei Chen
PURPOSE: Some studies have suggested that BAFF and BAFFR are highly expressed in the central nervous system (CNS) and participate in inflammatory and immune associated diseases. However, whether BAFF and BAFFR are involved in the pathogenesis of epilepsy remains unknown. This study aimed to investigate the expression of BAFF and BAFFR proteins in the brains of patients with temporal lobe epilepsy (TLE) and in a pilocarpine-induced rat model of TLE to identify possible roles of the BAFF-BAFFR signaling pathway in epileptogenesis...
April 8, 2017: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28441581/design-combinatorial-synthesis-and-biological-evaluations-of-novel-3-amino-1-1-aryl-1h-1-2-3-triazol-5-yl-methyl-2-oxospiro-benzo-a-pyrano-2-3-c-phenazine-1-3-indoline-2-carbonitrile-antitumor-hybrid-molecules
#9
Yuanyuan Lu, Linlin Wang, Xiaobing Wang, Tao Xi, Jianmin Liao, Zhixiang Wang, Feng Jiang
A combinatorial chemical library of fifty-nine novel 3-amino-1'-((1-aryl-1H-1,2,3-triazol-5-yl)methyl)-2'-oxospiro[benzo[a]pyrano[2,3-c]phenazine-1,3'-indoline]-2-carbonitrile, designed as hybrid molecules of phenazine, pyran, indole and 1,2,3-triazole pharmacophores, were constructed in this study. Cytotoxic evaluation indicated that some compounds exhibited moderate cytotoxicity against HCT116, MCF7, HepG2 and A549 cancer cell lines in vitro, in which compound 36 was found to have best antiproliferative activity against the A549 cancer cell line with IC50 value of 5...
April 18, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28441432/dysbiosis-of-intestinal-microbiota-and-decrease-in-paneth-cell-antimicrobial-peptide-level-during-acute-necrotizing-pancreatitis-in-rats
#10
Jing Chen, Chunlan Huang, Jingjing Wang, Hui Zhou, Yingying Lu, Lihong Lou, Junyuan Zheng, Ling Tian, Xingpeng Wang, Zhongwei Cao, Yue Zeng
OBJECTIVES: Intestinal barrier dysfunction plays an important role in acute necrotizing pancreatitis (ANP) and intestinal microbiota dysbiosis was involved in intestinal barrier failure. Paneth cells protect intestinal barrier and are associated with intestinal microbiota. Here, we investigated changes in intestinal microbiota and antimicrobial peptides of Paneth cells in ileum during ANP. METHODS: Rats with ANP were established by retrograde injection of 3.5% sodium taurocholate into biliopancreatic duct and sacrificed at 24h and 48h, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28440917/atf3-inhibits-the-inflammation-induced-by-mycoplasma-pneumonia-in-vitro-and-in-vivo
#11
Jing Wang, Wei Cheng, Zhen Wang, Lihong Xin, Wen Zhang
OBJECTIVES: Activating transcription factor-3 (ATF3) is a key regulator of inflammatory responses. We aimed to investigate the effects and mechanisms of ATF3 on the inflammatory cytokines are induced by Mycoplasma pneumonia (MP). STUDY DESIGN: RAW264.7 and mouse peritoneal macrophages were exposed to various time with or without MP infection (3, 6, 12, 24, and 48 h), and detect the expression of ATF3. Adenovirus-expression of ATF3 (Ad/ATF3) or Ad/βgal was transfected into cells which were exposed to MP for 48 h, RT-PCR and ELISA was used to evaluate the expression and secretion of TNF-α, IL-1β, IL-6, and IL-18...
April 25, 2017: Pediatric Pulmonology
https://www.readbyqxmd.com/read/28440797/potential-mechanisms-of-microrna-141-3p-to-alleviate-chronic-inflammatory-pain-by-down-regulation-of-downstream-target-gene-hmgb1-in-vitro-and-in-vivo-studies
#12
W-S Shen, X-Q Xu, N-N Zhai, Z-S Zhou, J Shao, Y-H Yu
The present study aimed to investigate the potential role of microRNA-141-3p (miR-141-3p) in chronic inflammatory pain (CIP) by targeting HMGB1 gene. In the in vitro study, BV2 microglial cells were selected and assigned into blank, lipopolysaccharide (LPS), miR-141-3p mimics, mimics control, miR-141-3p inhibitor, inhibitor control, miR-141-3p mimics+LPS, mimics control+ LPS, miR-141-3p inhibitor+LPS, and inhibitor control+LPS groups. Ninety-six rats were randomly divided into 8 groups (12 rats in each group): blank control, model control, negative control (NC), miR-141-3p mimics+CFA, mimics control+CFA, HMGB1 shRNA+CFA, HMGB1 NC+CFA, and miR-141-3p mimics+HMGB1 shRNA+CFA groups...
April 25, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28440738/restricted-feeding-modulates-the-daily-variations-of-liver-glutamate-dehydrogenase-activity-expression-and-histological-location
#13
Olivia Vázquez-Martínez, Isabel Méndez, Isaías Turrubiate, Héctor Valente-Godínez, Moisés Pérez-Mendoza, Paola García-Tejada, Mauricio Díaz-Muñoz
Glutamate dehydrogenase is an important enzyme in the hepatic regulation of nitrogen and energy metabolism. It catalyzes one of the most relevant anaplerotic reactions. Although its relevance in liver homeostasis has been widely described, its daily pattern and responsiveness to restricted feeding protocols has not been studied. We explored the daily variations of liver glutamate dehydrogenase transcription, protein, activity, and histochemical and subcellular location in a protocol of daytime food synchronization in rats...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28440736/levistilide-a-inhibits-angiogenesis-in-liver-fibrosis-via-vascular-endothelial-growth-factor-signaling-pathway
#14
Zhi-Min Zhao, Hong-Liang Liu, Xin Sun, Tao Guo, Li Shen, Yan-Yan Tao, Cheng-Hai Liu
Levistilide A (C24H28O4, molecular weight = 380.48) derived from Angelica sinensis (Danggui) has been reported to inhibit hepatic stellate cell proliferation. This study investigated the effects of levistilide A on liver fibrosis relating to angiogenesis, particularly on the characteristic change in liver sinusoidal endothelial cells. LX-2 cells were activated by TGF-β1, and the human hepatic sinusoidal endothelial cells (HHSECs) were induced by endothelial cell growth supplement. Cell viability was detected using a methylthiazoldiphenyl-tetrazolium bromide assay; F-actin was visualized through the fluorescence probe method; cell proliferation was examined using the EdU kit; antiangiogenesis activity was assessed using the tube formation assay and transgenic zebrafish model...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28440528/-effect-of-asct2-gene-knock-down-by-shrna-on-biological-behaviors-of-colorectal-cancer-cells
#15
Canfeng Cai, Bing Zeng, Jun Zeng, Haiyang Xin, Chaoming Tang
OBJECTIVE: To investigate the effect of ASCT2 gene (glutamine transporter) knock-down by shRNA on biological behaviors of colorectal cancer cells. METHODS: shRNA was transfected into colorectal cancer cells Lovo and SW480 to knockdown ASCT2 mediated by Lipofectamine 2000. Reverse transcription-PCR and Western blot were used to examine the mRNA and protein expression of ASCT2. MTT and transwell assay were used to determine the proliferation and invasiveness of Lovo and SW480 cells...
April 25, 2017: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/28440512/astemizole-protects-against-human-umbilical-vein-endothelial-cell-injury-induced-by-hydrogen-peroxide-via-the-p53-signaling-pathway
#16
Jia-Nan Tian, Xiao-Dong Shi, Xiao-Kun Wang, Shuang Wang, Jing-Xue Xu, Chun-Xiao Yang
Astemizole has gained attention as an antineoplastic drug that targets important ion channels. The present study aimed to investigate the protective effects of astemizole against hydrogen peroxide (H2O2)‑induced oxidative damage to human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated with astemizole (0.5 and 1 µM) for 12 h, then exposed to H2O2 (200 µM) for 12 h. Cell viability was measured using the MTT assay. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px), reactive oxygen species (ROS) and apoptotic percentage were determined...
April 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440510/migration-of-oral-squamous-cell-carcinoma-cells-are-induced-by-hgf-c-met-signalling-via-lamellipodia-and-filopodia-formation
#17
Hiroki Yasui, Yuichi Ohnishi, Masahiro Nakajima, Masami Nozaki
The activation of receptor tyrosine kinases (RTKs) results in cellular effects including cell proliferation, survival, migration and invasion; RTKs also play an important role in tumourigenesis. It has been reported that EGFR signalling controls the migration of oral squamous cell carcinoma (OSCC) SAS and HSC3 cells but not of HSC4 cells, although the proliferation of HSC4 cells is regulated by EGF/EGFR. In the present study, we investigated the roles of EGFR and the c-Met signalling pathway in cell migration via filopodia and lamellipodia formation, which may be prerequisites for migration...
April 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28440508/expression-levels-of-microrna%C3%A2-455-and-its-potential-functions-by-targeting-igf%C3%A2-1r-in-melanoma
#18
Hui Wang, Liang Yu, Xiujuan Shan
Melanoma has the highest fatality and malignancy of all skin cancers. In cancer, microRNAs (miRNAs) act as tumor suppressors or oncogenes, and inactivation of oncogenic miRNAs or restoration of tumor suppressor miRNAs has potential for cancer treatment. In the present study, miR‑455 expression levels were examined in melanoma tissues and cell lines, and miR‑455 was significantly downregulated in melanoma compared with matched normal tissues or normal epidermal melanocytes. In addition, increasing miR‑455 expression in melanoma cells reduced cell proliferation and invasion...
April 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440506/association-between-high-mobility-group-box%C3%A2-1-protein-expression-and-cell-death-in-acute-pancreatitis
#19
Enjun Gao, Yanfeng Jiang, Zhituo Li, Dongbo Xue, Weihui Zhang
The present study used caerulein stimulation of AR42J rat pancreatic cells as an in vitro acute pancreatitis (AP) model to investigate proteins differentially expressed in apoptosis and necrosis. AR42J cells were stimulated with 10‑8mol/l caerulein and incubated for 24 h. Apoptosis and necrosis were detected using flow cytometry. The sorted Annexin V‑positive cells (apoptotic) and the Annexin V/propidium iodide double‑positive cells (necrotic) were analysed using proteomics. Results showed that numerous proteins were differentially expressed in these 2 groups...
April 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28440502/marine-actinomycete-crude-extracts-with-potent-trail-resistance-overcoming-activity-against-breast-cancer-cells
#20
Mohammed I Y Elmallah, Olivier Micheau, Mennat Allah G Eid, Ali M S Hebishy, Mohamed S Abdelfattah
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent, as it can kill tumor cells selectively. In our search of bioactive natural products to overcome TRAIL-resistance, we isolated 47 actinomycete strains from different sediments and seawater samples collected from the Red Sea coast in Egypt and found four crude extracts (EGY1, EGY3, EGY24 and EGY34) displaying TRAIL sensitizing activity in the resistant breast cancer cell line MDA-MB-231. None of these crude extracts exhibited cytotoxic effect on normal mouse embryonic fibroblasts (MEF), with the exception of EGY34...
April 21, 2017: Oncology Reports
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