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https://www.readbyqxmd.com/read/29054095/ginkgolide-b-and-bilobalide-ameliorate-neural-cell-apoptosis-in-%C3%AE-synuclein-aggregates
#1
Jun Hua, Nuo Yin, Beibei Yang, Ji Zhang, Jianhua Ding, Yi Fan, Gang Hu
The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the α-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human α-Syn, but not α-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay...
October 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29054094/runx3-inhibits-the-proliferation-and-metastasis-of-gastric-cancer-through-regulating-mir-182-hoxa9
#2
Junyan Yu, Xiangyang Tian, Jianlan Chang, Ping Liu, Rong Zhang
OBJECTIVE: This study intended to explore the molecular mechanism of RUNX3 in inhibiting the process of migration and proliferation of gastric cancer (GC) cells. METHODS: The overexpressed plasmids of RUNX3 and the interfering siRNA of RUNX3 were transfected into GC cells. Then, qRT-PCR and western blot were performed to identify the expression level of RUNX3 and miR-182 in tumors and adjacent tissues respectively. ChIP and luciferase assay were performed to detect the relationship between RUNX3 and miR-182 as well as miR-182 and HOXA9...
October 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29053988/intermedin-reduces-neointima-formation-by-regulating-vascular-smooth-muscle-cell-phenotype-via-camp-pka-pathway
#3
Qing Zhu, Xian-Qiang Ni, Wei-Wei Lu, Jin-Sheng Zhang, Jin-Ling Ren, Di Wu, Yao Chen, Lin-Shuang Zhang, Yan-Rong Yu, Chao-Shu Tang, Yong-Fen Qi
BACKGROUND AND AIMS: Vascular smooth muscle cell (VSMC) dedifferentiation contributes to neointima formation, which results in various vascular disorders. Intermedin (IMD), a cardiovascular paracrine/autocrine polypeptide, is involved in maintaining circulatory homeostasis. However, whether IMD protects against neointima formation remains largely unknown. The purpose of this study is to investigate the role of IMD in neointima formation and the possible mechanism. METHODS: IMD1-53 (100ng/kg/h) or saline water was used on rat carotid-artery balloon-injury model...
October 9, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29053877/trpv4-regulates-vascular-endothelial-permeability-during-colonic-inflammation-in-dextran-sulphate-sodium-induced-murine-colitis
#4
Kenjiro Matsumoto, Riho Yamaba, Ken Inoue, Daichi Utsumi, Takuya Tsukahara, Kikuko Amagase, Makoto Tominaga, Shinichi Kato
BACKGROUND AND PURPOSE: The transient receptor potential vanilloid 4 (TRPV4) is a nonselective cation channel involved in physical sensing in various tissue types. The present study aimed to elucidate the function and expression of TRPV4 in colonic vascular endothelial cells during dextran sulphate sodium (DSS)-induced colitis. EXPERIMENTAL APPROACH: The role of TRPV4 in the progression of colonic inflammation was examined in the 2 % DSS-induced murine colitis model using immunohistochemical analysis, western blotting, and Evans blue dye extrusion assay...
October 20, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29053735/characterization-of-the-first-knock-out-aldh7a1-zebrafish-model-for-pyridoxine-dependent-epilepsy-using-crispr-cas9-technology
#5
Nikita Zabinyakov, Garrett Bullivant, Feng Cao, Matilde Fernandez Ojeda, Zheng Ping Jia, Xiao-Yan Wen, James J Dowling, Gajja S Salomons, Saadet Mercimek-Andrews
Pyridoxine dependent epilepsy (PDE) is caused by likely pathogenic variants in ALDH7A1 (PDE-ALDH7A1) and inherited autosomal recessively. Neurotoxic alpha-amino adipic semialdehyde (alpha-AASA), piperideine 6-carboxylate and pipecolic acid accumulate in body fluids. Neonatal or infantile onset seizures refractory to anti-epileptic medications are clinical features. Treatment with pyridoxine, arginine and lysine-restricted diet does not normalize neurodevelopmental outcome or accumulation of neurotoxic metabolites...
2017: PloS One
https://www.readbyqxmd.com/read/29053518/role-of-homeodomain-interacting-protein-kinase-2-in-the-pathogenesis-of-tissue-fibrosis-in-keloid-derived-keratinocytes
#6
Yi-Xuan Zhao, Guo-You Zhang, An-Yuan Wang, Ya-Hong Chen, Da-Mu Lin, Qing-Feng Li
Epithelial-mesenchymal transition (EMT) plays a critical role in fibrotic keloid formation, which is characterized by excessive collagen and extracellular matrix synthesis and deposition. Growing evidence suggests that the serine/threonine kinase homeodomain-interacting protein kinase 2 (HIPK2) acts upstream of several major fibrosis signaling pathways; however, the role of HIPK2 in the keloid fibrogenesis remains unknown. In the current study, we investigated the roles of HIPK2 in the pathogenesis of keloids...
October 19, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/29053434/alpha-gal-inactivated-heart-valve-bioprostheses-exhibit-an-anti-calcification-propensity-similar-to-knockout-tissues
#7
Filippo Naso, Ugo Stefanelli, Edward Buratto, Giovanna Lazzari, Andrea Perota, Cesare Galli, Alessandro Gandaglia
BACKGROUND: Glutaraldehyde (GLA) has been used to crosslink bioprosthetic heart valve (BHVs) tissues to enhance their stability, besides ensuring a satisfactory degree of immunological tolerance. Unfortunately, GLA fixation does not guarantee a complete tissue biocompatibility of BHVs in currently used devices. The interaction between preformed human anti-alpha-Gal antibody and alpha-Gal antigens promotes the calcification of GLA-treated alpha-Gal-positive tissue. Recently, an alarming correlation between the presence of the alpha-Gal epitope and a premature BHVs degeneracy was reported...
October 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29053012/the-ratio-of-thioredoxin-keap1-protein-level-is-a-predictor-of-distant-metastasis-in-colorectal-cancer
#8
Liang-Che Chang, Chung-Wei Fan, Wen-Ko Tseng, Hui-Ping Chein, Tsan-Yu Hsieh, Jim-Ray Chen, Cheng-Cheng Hwang, Chung-Ching Hua
AIM: Thioredoxin can reduce the cysteine group of Keap1 which could induce proteasome degradation of Nrf2, PGAM5 and Bcl-xL. Nrf2 regulates redox balance and Bcl-xL is anti-apoptotic and both are important in tumor progression. METHODS: The protein levels of Keap1, thioredoxin, PGAM5 and Bcl-xL in the normal and tumor tissues of 64 subjects with colorectal cancer (CRC) were determined by western blot. RESULTS: The tumor had higher Keap1 but lower PGAM5s and Bcl-xL protein expression than the normal tissue...
October 20, 2017: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29052839/effects-of-microrna-206-and-its-target-gene-igf-1-on-sevoflurane-induced-activation-of-hippocampal-astrocytes-in-aged-rats-through-the-pi3k-akt-creb-signaling-pathway
#9
Tie-Jun Liu, Bin Wang, Qun-Xi Li, Xiao-Liu Dong, Xiao-Liang Han, Shu-Bo Zhang
The study aims to explore the effects of microRNA-206 (miR-206) targeting IGF-1 on the activation of hippocampal astrocytes in aged rats induced by sevoflurane through the PI3K/AKT/CREB signaling pathway. Wistar rats and astrocytes were divided into the normal/blank, sham/negative control (NC), sevoflurane (sevo), miR-206 mimics + sevo, miR-206 inhibitors + sevo, miR-206 NC + sevo, IGF-1 shRNA + sevo and miR-206 inhibitors + IGF-1 shRNA + sevo groups. The Morris water maze test was exhibited to assess the cognitive functions...
October 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29052767/acute-regulation-of-multidrug-resistance-associated-protein-2-localization-and-activity-by-camp-and-estradiol-17%C3%AE-d-glucuronide-in-rat-intestine-and-caco-2-cells
#10
Guillermo Nicolás Tocchetti, Agostina Arias, Maite Rocío Arana, Juan Pablo Rigalli, Camila Juliana Domínguez, Felipe Zecchinati, María Laura Ruiz, Silvina Stella Maris Villanueva, Aldo Domingo Mottino
Multidrug resistance-associated protein 2 (MRP2) is an ATP-dependent transporter expressed at the brush border membrane of the enterocyte that confers protection against absorption of toxicants from foods or bile. Acute, short-term regulation of intestinal MRP2 activity involving changes in its apical membrane localization was poorly explored. We evaluated the effects of dibutyryl-cAMP (db-cAMP), a permeable analog of cAMP, and estradiol-17β-D-glucuronide (E217G), an endogenous derivative of estradiol, on MRP2 localization and activity using isolated rat intestinal sacs and Caco-2 cells, a model of human intestinal epithelium...
October 20, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29052569/analysis-of-the-expression-of-angioarchitecture-related-factors-in-patients-with-cerebral-arteriovenous-malformation
#11
Guang-Zhong Chen, Yu Ke, Kun Qin, Meng-Qi Dong, Shao-Jian Zeng, Xiao-Feng Lin, Sheng-Quan Zhan, Kai Tang, Chao Peng, Xiao-Wen Ding, Dong Zhou
BACKGROUND: Cerebral arteriovenous malformation (cAVM) is a type of vascular malformation associated with vascular remodeling, hemodynamic imbalance, and inflammation. We detected four angioarchitecture-related cytokines to make a better understanding of the potential aberrant signaling in the pathogenesis of cAVM and found useful proteins in predicting the risk of cerebral hemorrhage. METHODS: Immunohistochemical analysis was conducted on specimens from twenty patients with cAVM diagnosed via magnetic resonance imaging and digital subtraction angiography and twenty primary epilepsy controls using antibodies against vascular endothelial growth factor receptor-2 (VEGFR-2), matrix metalloproteinase-9 (MMP-9), vascular cell adhesion molecule (VCAM-1), and endothelial nitric oxide synthase (eNOS)...
October 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/29052531/pten-expression-is-upregulated-by-a-rna-binding-protein-rbm38-via-enhancing-its-mrna-stability-in-breast-cancer
#12
Xu-Jie Zhou, Jing Wu, Liang Shi, Xiao-Xia Li, Lei Zhu, Xi Sun, Jia-Yi Qian, Ying Wang, Ji-Fu Wei, Qiang Ding
BACKGROUND: PTEN (phosphatase and tensin homolog gene on chromosome 10), a well-characterized tumor suppressor, is a key regulator of the phosphatidylinositol-3-kinase (PI3K)/AKT pathway involved in cell survival, metastasis and cell renewal. PTEN expression is closely related to the phenotype, prognosis and drug selection in breast cancer. It is mainly regulated by transcriptional and post-transcriptional modifications. RNA binding motif protein 38 (RBM38), an RNA-binding protein (RBP) and a target of P53 family, plays a crucial role in the regulation of cellular processing, especially in post-transcription regulation and gene transcription...
October 19, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29052525/epigenetic-mediated-zinc-finger-protein-671-downregulation-promotes-cell-proliferation-and-tumorigenicity-in-nasopharyngeal-carcinoma-by-inhibiting-cell-cycle-arrest
#13
Jian Zhang, Xin Wen, Na Liu, Ying-Qin Li, Xin-Ran Tang, Ya-Qin Wang, Qing-Mei He, Xiao-Jing Yang, Pan-Pan Zhang, Jun Ma, Ying Sun
BACKGROUND: Epigenetic abnormalities play important roles in nasopharyngeal cancer (NPC), however, the epigenetic changes associated with abnormal cell proliferation remain unclear. METHODS: We detected epigenetic change of ZNF671 in NPC tissues and cell lines by bisulfite pyrosequencing. We evaluated zinc finger protein 671 (ZNF671) expression in NPC cell lines and clinical tissues using real-time PCR and western blotting. Then, we established NPC cell lines that stably overexpressed ZNF671 and knocked down ZNF671 expression to explore its function in NPC in vitro and in vivo...
October 19, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29052520/upregulation-of-dars2-by-hbv-promotes-hepatocarcinogenesis-through-the-mir-30e-5p-mapk-nfat5-pathway
#14
Xian Qin, Changsheng Li, Tao Guo, Jing Chen, Hai-Tao Wang, Yi-Tao Wang, Yu-Sha Xiao, Jun Li, Pengpeng Liu, Zhi-Su Liu, Quan-Yan Liu
BACKGROUND: Infection with the hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma (HCC). The osmoregulatory transcription factor nuclear factor of activated T-cells 5 (NFAT5) has been shown to play an important role in the development of many types of human cancers. The role of NFAT5 in HBV-associated HCC has never previously been investigated. METHODS: We compared expression profiles of NFAT5, DARS2 and miR-30e-5p in HCC samples, adjacent nontumor tissues and different hepatoma cell lines by quantitative real-time polymerase chain reaction and /or Western blot...
October 19, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29052509/a-yin-yang-1-mir-30a-regulatory-circuit-modulates-autophagy-in-pancreatic-cancer-cells
#15
Chuang Yang, Jing-Jing Zhang, Yun-Peng Peng, Yi Zhu, Ling-Di Yin, Ji-Shu Wei, Wen-Tao Gao, Kui-Rong Jiang, Yi Miao
BACKGROUND: Autophagy is a highly regulated biological process that mediates the degradation of intracellular components. It is required for tumor cell metabolism and homeostasis. Yin-Yang 1 (YY1) has been reported to be involved in autophagy in several carcinomas. However, its role in autophagy in pancreatic cancer, one of the deadliest human malignancies, is unknown. Here, we investigated the function of YY1 in pancreatic cancer cells autophagy and its mechanisms of action. METHODS: The activity of cells undergoing autophagy was assessed using transmission electron microscopy, immunofluorescence, and Western blotting...
October 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29052402/-effect-of-qibai-pingfei-capsule-medicated-serum-on-protein-expressions-of-katp-channel-in-pulmonary-arterial-smooth-muscle-cells-via-nitric-oxide
#16
Jie Zhu, Ze-Geng Li, Bao-Qin Wang, Xiang-Li Tong, Qing-He Peng
To investigate the ATP-sensitive potassium channel (KATP channel) protein expressions during different periods under hypoxia condition and explore the effect of Qibai Pingfei capsule medicated serum (hereinafter referred to as QBPF) on the correlation between the protein expressions of KATP channel and nitric oxide in rat pulmonary arterial smooth muscle cells(PASMCs). Qibai Pingfei capsules were given to SD rats via continuous gavage for 10 days to obtain QBPF. Primary rats PASMCs were cultured by the direct adherent culture method...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052401/-relaxation-effect-of-buddleoside-combined-with-luteolin-on-isolated-vessels-in-vivo-and-its-mechanism
#17
Yao Yang, Bo Chen, Kai-Lun Liang, Jie Su, Su-Hong Chen, Gui-Yuan Lv
To study the relaxation effect of buddleoside combined with luteolin on aortic rings in SD rats and its mechanism. The effect of buddleoside alone(7.5×10⁻⁶g•mL⁻¹), luteolin alone(7.5×10⁻⁶g•mL⁻¹) and the combination of buddleoside and luteolin(1∶4) on norepinephrine-induced contractility of complete, endothelium-denuded, and L-NAME and indomethacin-pretreated thoracic aorta in SD rats were observed in the in vitro ring tension test. Western blot was used to detect p-Akt and p-eNOS protein expressions in the thoracic aorta...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052400/-protective-effect-of-ginsenoside-rb%C3%A2-on-doxorubicin-induced-myocardial-autophagy
#18
Long-Fei Li, Zeng-Chun Ma, Yu-Guang Wang, Xiang-Lin Tang, Hong-Ling Tan, Cheng-Rong Xiao, Yue Gao
Ginsenoside Rb₁ (Rb₁), which is one of the main ingredients derived from Panax ginseng, has been found to have extensive pharmacological activities including antioxidant, anti-inflammatory, anticancer properties. In this study, the effect of Rb₁ on doxorubicin-induced myocardial autophagy was studied with H9c2 as the study object. CCK-8 method, transmission electron microscope observation, fluorescence staining observation and Western blot were used to detect changes in H9c2 cell proliferation and autophagy after treatment...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052399/-effect-of-total-flavonoids-in-scutellaria-barbata-in-mediating-autophagy-in-tumor-cells-via-pi3k-akt-mtor-pathway
#19
Ming Chen, Ju-Tao Wang, Zhen-Ni Wu, Man-Yan Hu, Hua-Wu Gao
To investigate the effect of the total flavonoids in Scutellaria barbata(TF-SB) against autophagy in tumor cells in vivo, and further determine whether the mechanism is correlated with the PI3K/AKT/mTOR pathway, which lead to autophagy-induced tumor cell death. Melanoma-bearing mice were prepared and divided into control group, rapamycin group (Rap 1.5 mg•kg⁻¹), and high, middle and low-dose TF-SB (200, 100, 50 mg•kg⁻¹) groups. The groups were given drugs once a day for successively 11 days. The inhibitory effect of TF-SB on the growth of melanoma was determined by measuring tumor volume and tumor inhibition rate...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052243/wnt-%C3%AE-catenin-signalling-pathway-mediated-aberrant-hippocampal-neurogenesis-in-kainic-acid-induced-epilepsy
#20
Zhengyi Qu, Fang Su, Xueting Qi, Jianbo Sun, Hongcai Wang, Zhenkui Qiao, Hong Zhao, Yulan Zhu
Temporal lobe epilepsy is a chronic disorder of nerve system, mainly characterized by hippocampal sclerosis with massive neuronal loss and severe gliosis. Aberrant neurogenesis has been shown in the epileptogenesis process of temporal lobe epilepsy. However, the molecular mechanisms underlying aberrant neurogenesis remain unclear. The roles of Wnt signalling cascade have been well established in neurogenesis during multiple aspects. Here, we used kainic acid-induced rat epilepsy model to investigate whether Wnt/β-catenin signalling pathway is involved in the aberrant neurogenesis in temporal lobe epilepsy...
October 19, 2017: Cell Biochemistry and Function
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