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https://www.readbyqxmd.com/read/29906814/magnoflorine-enhances-lps-activated-pro-inflammatory-responses-via-myd88-dependent-pathways-in-u937-macrophages
#1
Md Areeful Haque, Ibrahim Jantan, Hemavathy Harikrishnan, Siti Mariam Abdul Wahab
Magnoflorine, a major bioactive metabolite isolated from Tinospora crispa, has been reported for its diverse biochemical and pharmacological properties. However, there is little report on its underlying mechanisms of action on immune responses, particularly on macrophage activation. In this study, we aimed to investigate the effects of magnoflorine, isolated from T. crispa on the pro-inflammatory mediators generation induced by LPS and the concomitant NF- κ B, MAPKs, and PI3K-Akt signaling pathways in U937 macrophages...
June 15, 2018: Planta Medica
https://www.readbyqxmd.com/read/29906745/long-noncoding-rna-linc00460-targets-mir-539-mmp-9-to-promote-meningioma-progression-and-metastasis
#2
Hongshun Xing, Shilei Wang, Quancai Li, Yongqian Ma, Peng Sun
Emerging evidence reveal that long noncoding RNAs (lncRNAs) participates in the epigenetic regulation of pathophysiological process. However, the deepgoing role of lncRNAs on meningioma is still unclear. In present study, we investigate the roles of lncRNA LINC00460 in meningeoma tissue and uncover its molecular mechanism. Results revealed that LINC00460 expression level was significantly up-regulated in meningeoma tissues and malignant meningeoma cell lines (IOMM-Lee, CH157-MN). Mechanically, loss-of-function assays showed that LINC00460 knockdown significantly suppressed the proliferation ability, increased the apoptosis and decreased the proteins (MMP-2, MMP-9, ZEB1) expression...
June 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29906550/pituitary-adenylate-cyclase-activating-polypeptide-pacap1-38-exerts-both-pro-and-anti-apoptotic-effects-on-postnatal-retinal-development-in-rat
#3
Nyisztor Zsolt, Denes Viktoria, Kovacs-Valasek Andrea, Hideg Orsolya, Berta Gergely, Gabriel Robert
PACAP1-38, a ubiquitous and multifunctional regulator has been in the focus of neurotoxicity research due to its impressive neuroprotective potential. Although the literature extensively demonstrated its repressive effect on the apoptotic machinery in neurodegenerative models, there is a striking absence of analysis on its role in normal development. We performed quantitative analyses on caspase activity in developing retina upon 100, 50, 25 or 1 pmol intravitreal PACAP1-38 injection from postnatal day 1 (P1) through P7 in Wistar rats...
June 12, 2018: Neuroscience
https://www.readbyqxmd.com/read/29906536/mahuang-decoction-mitigates-airway-inflammation-and-regulates-il-21-stat3-signaling-pathway-in-rat-asthma-model
#4
Yu He, Xiaohui Lou, Zhan Jin, Li Yu, Ling Deng, Haitong Wan
ETHNOPHARMACOLOGICAL RELEVANCE: Nowadays, bronchial asthma is still a severe disease threatening human health, and it is incumbent upon us to seek effective therapeutic drugs. Mahuang decoction (MHD), a classic famous Chinese prescription, has been used for thousands of years to prevent phlegm from forming, stop coughing and relieve asthma, but the relevant mechanism has not been thoroughly clarified. This study aims to investigate the anti-airway inflammation effect of MHD and the possible molecular mechanism underlying IL21/STAT3 signaling pathway, so as to provide guidance for the treatment of MHD on bronchial asthma...
June 12, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29906461/sphingomyelin-phosphodiesterase-1-smpd1-mediates-the-attenuation-of-myocardial-infarction-induced-cardiac-fibrosis-by-astaxanthin
#5
Yu Shi, Peng Lin, Xiaoning Wang, Guangmei Zou, Kefeng Li
Uncontrolled cardiac fibrosis following myocardial infarction (MI) is a critical pathological change leading to heart failure. Current pharmacotherapies are limited by unsatisfactory efficacy and undesired systemic side effects. Astaxanthin (ASX) is a natural carotenoid with strong antioxidant and anti-inflammatory activities. The effects of ASX on MI-induced cardiac fibrosis and the underlying mechanisms remain largely unknown. In this study, after the establishment of MI model, mice were administrated with ASX (200 mg/kg⋅d) for 4 weeks...
June 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29906460/loss-of-virulence-in-nad-p-h-cytochrome-b5-oxidoreductase-deficient-leishmania-major
#6
Aditi Mukherjee, Ayan Adhikari, Priya Das, Saroj Biswas, Supratim Mukherjee, Subrata Adak
Leishmania promastigotes have the ability to synthesize essential polyunsaturated fatty acids de novo and can grow in lipid free media. Recently, we have shown that NAD(P)H cytochrome b5 oxidoreductase (Ncb5or) enzyme in Leishmania acts as the redox partner for Δ12 fatty acid desaturase, which catalyses the conversion of oleate to linoleate. So far, the exact role of Leishmania derived linoleate synthesis is still incomplete in the literature. The viability assay by flow cytometry as well as microscopic studies suggests that linoleate is an absolute requirement for Leishmania promastigote survival in delipidated media...
June 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29906453/immunization-with-tumor-neoantigens-displayed-on-t7-phage-nanoparticles-elicits-plasma-antibody-and-vaccine-draining-lymph-node-b-cell-responses
#7
Girja S Shukla, Yu-Jing Sun, Stephanie C Pero, Giselle S Sholler, David N Krag
The aim of this preclinical study was to evaluate T7 bacteriophage as a nanoparticle platform for expression of neoantigens that could allow rapid generation of vaccines for potential studies in human cancer patients. We have generated recombinant T7 phage vaccines carrying neoepitopes derived from mutated proteins of B16-F10 melanoma tumor cells. With the single mutated amino acid (AA) centered, peptides were expressed on the outer coat of T7 phage. All peptides with 11 and 34 AAs were successfully expressed...
June 12, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29906337/retained-differentiation-capacity-of-human-skeletal-muscle-satellite-cells-from-spinal-cord-injured-individuals
#8
Mladen Savikj, Maxwell A Ruby, Emil Kostovski, Per O Iversen, Juleen R Zierath, Anna Krook, Ulrika Widegren
Despite the well-known role of satellite cells in skeletal muscle plasticity, the effect of spinal cord injury on their function in humans remains unknown. We determined whether spinal cord injury affects the intrinsic ability of satellite cells to differentiate and produce metabolically healthy myotubes. We obtained vastus lateralis biopsies from eight spinal cord-injured and six able-bodied individuals. Satellite cells were isolated, grown and differentiated in vitro. Gene expression was measured by quantitative PCR...
June 2018: Physiological Reports
https://www.readbyqxmd.com/read/29905947/immunoreactivity-of-biochemically-purified-amandin-from-thermally-processed-almonds-prunus-dulcis-l
#9
Valerie D Zaffran, Shridhar K Sathe
Almond seeds were subjected to select thermal processing and amandin was purified from processed and unprocessed (control) seeds using cryoprecipitation. Amandin immunoreactivity was assessed using two murine monoclonal antibodies (mAbs)-4C10 and 4F10 detecting human IgE-relevant conformational and linear epitopes, respectively. Overall amandin immunoreactivity following thermal treatment ranged from 64.9% to 277.8% (4C10) and 81.3% to 270.3% (4F10). Except for autoclaving (121 °C, 15 psi, 30 min) and roasting (160 °C, 30 min), the tested processing conditions resulted in increased immunoreactivity as determined by mAbs 4C10 and 4F10-based enzyme-linked immunosorbent assays (ELISAs)...
June 15, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29905853/2-methoxyestradiol-inhibits-hypoxia-induced-scleroderma-fibroblast-collagen-synthesis-by-phosphatidylinositol-3-kinase-akt-mtor-signalling
#10
Xing Zhou, Chaofan Liu, Jinghao Lu, Lubing Zhu, Ming Li
Objectives: To investigate the mechanism of 2-methoxyestradiol (2-ME) in inhibiting hypoxia-induced collagen synthesis of fibroblasts in SSc. Methods: The expressions of hypoxia-inducible factor 1 alpha (HIF-1α) and connective tissue growth factor (CTGF) in skin specimens derived from SSc patients and healthy volunteers were examined by immunohistochemistry. HIF-1α was knocked down by lentiviral transduction, and SSc dermal fibroblasts cultured under normoxic (21% O2) or hypoxic (1% O2) condition were treated with PI3K inhibitor LY294002, rapamycin or 2-ME (25 μM)...
June 13, 2018: Rheumatology
https://www.readbyqxmd.com/read/29905572/cloning-expression-and-characterisation-of-a-cysteine-protease-from-trichinella-spiralis
#11
Yan Yan Song, Li Ang Wang, Hua Na Ren, Xin Qi, Ge Ge Sun, Ruo Dan Liu, Peng Jiang, Xi Zhang, Jing Cui, Zhong Quan Wang
Cysteine protease is a superfamily of widespread proteolytic enzymes and plays a major role in larval invasion, migration, exsheathing, survival and immune evasion in parasites. In the present study, the gene coding cysteine proteinase of the nematode Trichinella spiralis (Owen, 1835) was cloned into pQE-80L and subsequently expressed in E. coli JM109. The rTsCP was purified and its antigenicity was identified by Western blot and ELISA. Using anti-rTsCP serum the native TsCP was identified in muscle larval crude proteins...
May 29, 2018: Folia Parasitologica
https://www.readbyqxmd.com/read/29905412/myeloid-derived-suppressor-cell-accumulation-in-renal-cell-carcinoma-is-correlated-with-ccl2-il-17-and-il-18-expression-in-blood-and-tumors
#12
Xiaodong Guan, Zhiyu Liu, Jianhua Zhang, Xunbo Jin
BACKGROUND: Myeloid-derived suppressor cells (MDSC) play an important role in tumor-mediated immune evasion. Levels of MDSC in peripheral blood are increased in patients with cancer, correlating with cancer stage and outcome. Studies have confirmed the associations between MDSC and various cytokines in the peripheral blood of murine and human cancer hosts. However, little is known about the association between parenchymal MDSC subsets and cytokines, or the mechanism drawing MDSC into tumor parenchyma...
June 15, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29905411/anti-inflammatory-and-antioxidant-effects-of-mesenchymal-and-hematopoietic-stem-cells-in-a-rheumatoid-arthritis-rat-model
#13
Hanan Abdelmawgoud, Asmaa Saleh
BACKGROUND: Mesenchymal stem cells (MSCs) are of increased importance because of their capacity to counteract inflammation and suppress host immune responses. OBJECTIVES: The aim of the study was to compare the effectiveness of MSCs and hematopoietic stem cells (HSCs) in the treatment of rheumatoid arthritis (RA). MATERIAL AND METHODS: Paw swelling was assessed by measuring the thickness of the hind paws using a caliper. Cytokines - interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), and IL-10 - and rheumatoid factor (RF) were measured using enzyme-linked immunosorbent assay (ELISA) kits...
June 15, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29905408/ginkgolide-b-exerts-anti-inflammatory-and-chondroprotective-activity-in-lps-induced-chondrocytes
#14
Hejia Hu, Yan Li, Zengfeng Xin, Xiangfeng Zhanga
BACKGROUND: Osteoarthritis (OA) is one of degenerative and chronic diseases of articular joints. Articular cartilage is an avascular tissue, and its primary cellular component is chondrocytes. The main characteristic of OA is non-classic inflammation and cartilage degeneration. Ginkgolide B (GB) is a component of Ginkgo biloba which has diverse bioactivities. OBJECTIVES: The present study uses an in vitro experimental model to detect the underlying anti-inflammatory and chondroprotective effects of GB and provides a new way for future clinical therapy of OA...
June 13, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29905041/-comparative-study-on-biological-characteristics-between-different-generations-of-rabbit-nucleus-pulposus-cells
#15
Qi Chen, Fangfang Shi, Xi Yang, Limin Liu, Yueming Song
Objective: To research the biological characteristics of different generations of rabbit nucleus pulposus cells (NPCs) that were cultured with natural culture and subculture method. Methods: The thoracolumbar segments of New Zealand white rabbits (6-8 weeks old and weighing 1.5-2.5 kg) were obtained and nucleus pulposus were isolated from disc regions. And NPCs were harvested by enzymatic digestion from nucleus pulposus. Primary NPCs were counted as P0 generation...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29905011/low-expression-of-ww-domain-containing-oxidoreductase-associates-with-hepatocellular-carcinoma-aggressiveness-and-recurrence-after-curative-resection
#16
Chenhao Zhou, Wanyong Chen, Jialei Sun, Manar Atyah, Yirui Yin, Wentao Zhang, Lei Guo, Qinghai Ye, Qiongzhu Dong, Yi Shi, Ning Ren
WW domain-containing oxidoreductase (WWOX), which has a protein-interaction domain and is regarded to be a tumor suppressor, has been known to play an important role in anti-angiogenesis and cancer progression. This study aimed to investigate prognostic values of WWOX expression in hepatocellular carcinoma (HCC) patients after hepatectomy. Additionally, we intended to formulate a valuable prognostic nomogram for HCCs. 182 HCC patients who underwent hepatectomy from January 2009 to January 2010 were enrolled in our study...
June 14, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29904971/a-subset-of-walnut-allergic-adults-is-sensitized-to-walnut-11s-globulin-jug-r-4
#17
Mark A Blankestijn, Constance F den Hartog Jager, W Marty Blom, Henny G Otten, G Aard H de Jong, Marco Gaspari, Geert F Houben, André C Knulst, Kitty C M Verhoeckx
BACKGROUND: The role of sensitization to commercially available allergens of English walnut (Juglans regia) Jug r 1, 2 and 3 in walnut allergy has been previously investigated in walnut allergic adults and was unable to explain allcases of walnut allergy. OBJECTIVES: Identify recognized walnut allergens, other than the ones previously investigated (Jug r 1-3), in walnut allergic adults and determine the sensitization frequency and diagnostic value. METHODS: Three different in-house walnut extracts were prepared and analysed on SDS-PAGE blots to identify allergenic walnut proteins...
June 15, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29904952/baicalin-effects-on-rats-with-spinal-cord-injury-by-anti-inflammatory-and-regulating-the-serum-metabolic-disorder
#18
Shufeng Kang, Shizhao Liu, Hongzhu Li, Dapeng Wang, Xiangbei Qi
Baicalin had neuroprotective effects on inhibiting neuronal cell apoptosis induced by spinal cord ischemic injury. This study aimed to explore the protective effects of Baicalin on rats with spinal cord injury (SCI) and its mechanism of action. The recovery of spinal cord nerve function in rats was evaluated by the Basso, Beattie, and Bresnahan (BBB) score and the combine behavioral score (CBS). The expressions of cytokines tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 were detected by the enzyme-linked immunosorbent assay method...
June 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29904949/combination-with-mir-124a-improves-the-protective-action-of-bmscs-in-rescuing-injured-rat-podocytes-from-abnormal-apoptosis-and-autophagy
#19
Jiping Sun, Jing Lv, Wenjing Zhang, Lili Li, Jia Lv, Yingzhou Geng, Aiping Yin
This in vitro study was performed to identify the role of miR-124a in bone marrow stromal stem cells (BMSCs) therapy for H2 O2 -induced rat podocyte injury, and determine whether combination treatment with miR-124a could improve the protective effect of BMSCs. Cell viability of podocytes was detected by CCK-8 assay. Detection of ROS level, apoptotic rate, and autophagy rate was carried out using flow cytometry assays. Oxidative stress parameters were analyzed using the ELISA assays. MiR-124a and mRNA levels were determined using real-time PCR...
June 15, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29904942/inhibition-of-notch-signaling-pathway-enhanced-the-radiosensitivity-of-breast-cancer-cells
#20
Jian-Heng Peng, Xiao-Lin Wang, Liang Ran, Jun-Long Song, Zhi-Ting Zhang, Xin Liu, Hong-Yuan Li
This study aimed to investigate the effect of inhibiting the Notch signaling pathway on the radiosensitivity of breast cancer cells. Human breast cancer cell lines (MCF-7 and T47D) were selected and treated with radiation of different doses. Cells were treated with Gamma secretase inhibitor (GSI) to analyze the effects of GSI on the Notch signaling, which were detected by Immunofluorescence assay, RT-qPCR, and Western blot analysis. Besides, Transwell assay, Scratch test, colony formation assay, MTT assay, and flow cytometry were conducted to show the effects of GSI on the invasion and migration, survival fraction, cell viability, and apoptosis of MCF-7 and T47D cells after radiation therapy...
June 15, 2018: Journal of Cellular Biochemistry
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