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https://www.readbyqxmd.com/read/29471534/cypermethrin-promotes-lung-cancer-metastasis-via-modulation-of-macrophage-polarization-by-targeting-microrna-155-bcl6
#1
Fang Huang, Zhifeng Chen, Hanwen Chen, Wei Lu, Shujun Xie, Qing H Meng, Yihua Wu, Dajing Xia
Cypermethrin (CYM), a type II pyrethroid, is widely used as an insecticide for agriculture and household. Cumulative evidence indicates that acute and chronic exposure to CYM might cause a number of health problems, such as cancer and neuronal system diseases. However, the molecular mechanism underlying this pathology is not known. The main objective of our study was to define the effects of CYM on macrophages and the implication of such effects in cancer metastasis and the potential mechanism involved. The effects of CYM on the macrophages were evaluated by detecting the expression of M1 and M2 macrophage polarization markers through ELISA, quantitative RT-PCR and Western blot assay...
February 19, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29471396/involvement-of-epigenetic-modifications-of-gabaergic-interneurons-in-basolateral-amygdala-in-anxiety-like-phenotype-of-prenatally-stressed-mice
#2
Chunting Zhu, Min Liang, Yingchun Li, Xuejiao Feng, Juan Hong, Rong Zhou
Background: Prenatal stress is considered a risk factor for anxiety disorder. Downregulation in the expression of GABAergic gene, that is, glutamic acid decarboxylase 67, associated with DNA methyltransferase overexpression in GABAergic neurons has been regarded as a characteristic component of anxiety disorder. Prenatal stress has an adverse effect on the development of the basolateral amygdala, which is a key region in anxiety regulation. The aim of this study is to analyze the possibility of epigenetic alterations of GABAergic neurons in the basolateral amygdala participating in prenatal stress-induced anxiety...
February 20, 2018: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/29471302/socs3-is-upregulated-and-targeted-by-mir30a-5p-in-allergic-rhinitis
#3
Chun Yuan Zhao, Wei Wang, Hong Chao Yao, Xin Wang
BACKGROUND: Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 play important roles in T helper cell differentiation, which is involved with the pathologic mechanisms of allergic rhinitis (AR). The aim of this study was to evaluate the expression of SOCS1 and SOCS3 in AR and find their regulatory microRNAs (miRNAs) to provide a basis for the treatment of AR. METHODS: The expression of SOCS1 and SOCS3 were analyzed by real-time PCR, immunohistochemistry, and Western blot...
February 22, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29471244/two-sesquiterpene-aminoquinones-protect-against-oxidative-injury-in-hacat-keratinocytes-via-activation-of-ampk%C3%AE-erk-nrf2-are-ho-1-signaling
#4
Li Liu, Wei Wu, Jing Li, Wei-Hua Jiao, Li-Yun Liu, Jie Tang, Lei Liu, Fan Sun, Bing-Nan Han, Hou-Wen Lin
AIMS: To investigate the cytoprotective effects of two sesquiterpene aminoquinones isolated from the marine sponge Dysidea fragilis, Dysidaminone H (DA8) and 3'-methylamino-avarone (DA14), we examined their effects against hydrogen peroxide (H2 O2 )-induced oxidative injury in human keratinocyte cell line and elucidated the underlying mechanisms. MAIN METHODS: Cell viability was detected using a CCK-8 assay kit. Intracellular reactive oxygen species (ROS) production was measured by fluorescence of 2, 7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA)...
February 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29471107/pick1-deficiency-exacerbates-sepsis-associated-acute-lung-injury-and-impairs-glutathione-synthesis-via-reduction-of-xct
#5
Meizi Qian, Yingying Lou, Yixiu Wang, Minyuan Zhang, Qun Jiang, Yunchang Mo, Kunyuan Han, Shenhui Jin, Qinxue Dai, Yulong Yu, Zhen Wang, Junlu Wang
The role of oxidative stress has been well documented in the development of sepsis-induced acute lung injury (ALI). Protein interaction with C-kinase 1 (PICK1) participates in oxidative stress-related neuronal diseases. However, its function in lung infections and inflammatory diseases is not known. We therefore sought to investigate whether PICK1 is involved in sepsis-induced ALI. Cecal ligation and puncture (CLP) was performed in anesthetized wild type (WT) and PICK1 knock out (KO, PICK1-/- ) mice with C57BL/6 background...
February 19, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29471050/the-protein-protein-interactions-between-amsacta-moorei-entomopoxvirus-amev-protein-kinases-pks-and-all-viral-proteins
#6
Mehtap Danismazoglu, Remziye Nalcacioglu, Hacer Muratoglu, Zihni Demirbag
Entomopoxviruses are an important group of viruses infecting only insects. They belong to Poxviridae which infect both invertebrates and vertebrates, including humans. Protein kinases are known to have roles at virus morphogenesis, host selectivity, the regulation of cell division and apoptosis in some vertebrate poxviruses. In this study, 2 protein kinases (PKs) (AMV153 and AMV197) of Amsacta moorei entomopoxvirus (AMEV) were investigated for the interactions among 230 viral proteins using yeast two-hybrid system (Y2H)...
February 19, 2018: Virus Research
https://www.readbyqxmd.com/read/29470976/evaluation-of-methods-to-reduce-background-using-the-python-based-elisa_qc-program
#7
Rose P Webster, Cinder F Cohen, Fatima O Saeed, Hanna N Wetzel, William J Ball, Terence L Kirley, Andrew B Norman
Almost all immunological approaches [immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), Western blot], that are used to quantitate specific proteins have had to address high backgrounds due to non-specific reactivity. We report here for the first time a quantitative comparison of methods for reduction of background of commercial biotinylated antibodies using the Python-based ELISA_QC program. This is demonstrated using a recombinant humanized anti-cocaine monoclonal antibody. Several approaches, such as adjustment of the incubation time and the concentration of blocking agent, as also the dilution of secondary antibodies, have been explored to address this issue...
February 19, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29470947/small-and-large-intestine-express-a-truncated-dab1-isoform-that-assembles-in-cell-cell-junctions-and-co-localizes-with-proteins-involved-in-endocytosis
#8
María D Vázquez-Carretero, Pablo García-Miranda, María S Balda, Karl Matter, María J Peral, Anunciación A Ilundain
Disabled-1 (Dab1) is an essential intracellular adaptor protein in the reelin pathway. Our previous studies in mice intestine showed that Dab1 transmits the reelin signal to cytosolic signalling pathways. Here, we determine the Dab1 isoform expressed in rodent small and large intestine, its subcellular location and co-localization with clathrin, caveolin-1 and N-Wasp. PCR and sequencing analysis reveal that rodent small and large intestine express a Dab1 isoform that misses three (Y198 , Y200 and Y220 ) of the five tyrosine phosphorylation sites present in brain Dab1 isoform (canonical) and contains nuclear localization and export signals...
February 19, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29470852/high-quality-human-and-rat-spermatozoal-rna-isolation-for-functional-genomic-studies
#9
E Bianchi, A Stermer, K Boekelheide, M Sigman, S J Hall, G Reyes, E Dere, K Hwang
Sperm RNA is a sensitive monitoring endpoint for male reproductive toxicants, and a potential biomarker to assess male infertility and sperm quality. However, isolation of sperm RNA is a challenging procedure due to the heterogeneous population of cells present in the ejaculate, the low yield of RNA per spermatozoon, and the absence of 18S and 28S ribosomal RNA subunits. The unique biology of spermatozoa has created some uncertainty in the field about RNA isolation methods, indicating the need for rigorous quality control checks to ensure reproducibility of data generated from sperm RNA...
February 22, 2018: Andrology
https://www.readbyqxmd.com/read/29470830/strong-enhancement-by-igf1-r-antagonists-of-hepatocellular-carcinoma-cell-migration-inhibition-by-sorafenib-and-or-vitamin-k1
#10
Rosalba D'Alessandro, Maria Grazia Refolo, Catia Lippolis, Nicola Carella, Caterina Messa, Aldo Cavallini, Brian Irving Carr
PURPOSE: Emerging evidence indicates that combining Sorafenib with vitamin K1 (VK1) may result in a synergistic inhibition of hepatocellular carcinoma (HCC) cell migration and proliferation. Despite this synergy, its benefits may be limited due to drug resistance resulting from cross-talk with the tumor microenvironment. Insulin-like growth factor-1 (IGF1) signaling acts as an important modulator of HCC cell growth, motility and drug resistance. Therefore, we aimed to explore the effects of Sorafenib in combination with VK1 and/or IGF1-R antagonists on HCC cells...
February 22, 2018: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29470815/identification-of-mir-9-as-a-negative-factor-of-insulin-secretion-from-beta-cells
#11
Dongzhi Hu, Yi Wang, Haiyang Zhang, Dalu Kong
MicroRNA is a novel class of small noncoding RNA that has been implicated in a variety of physiological and pathological processes, including glucose homeostasis and diabetes mellitus. So far, a few studies have reported that miRNAs may be an important regulator in glucose-stimulated insulin secretion (GSIS) pathway. However, the role of miRNAs in this process remains unclear. The levels of miRNAs in mouse islets and MIN6 cells were determined by quantitative RT-PCR. Concentration of insulin was determined by ELISA, and the expression of the target protein was determined with western blot assay...
February 22, 2018: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29470513/offspring-sex-impacts-dna-methylation-and-gene-expression-in-placentae-from-women-with-diabetes-during-pregnancy
#12
Jacqueline Alexander, April M Teague, Jing Chen, Christopher E Aston, Yuet-Kin Leung, Steven Chernausek, Rebecca A Simmons, Sara E Pinney
AIMS/HYPOTHESIS: We hypothesized that diabetes during pregnancy (DDP) alters genome-wide DNA methylation in placenta resulting in differentially methylated loci of metabolically relevant genes and downstream changes in RNA and protein expression. METHODS: We mapped genome-wide DNA methylation with the Infinium 450K Human Methylation Bead Chip in term fetal placentae from Native American and Hispanic women with DDP using a nested case-control design (n = 17 pairs)...
2018: PloS One
https://www.readbyqxmd.com/read/29469690/non-canonical-escherichia-coli-transcripts-lacking-a-shine-dalgarno-motif-have-very-different-translational-efficiencies-and-do-not-form-a-coherent-group
#13
Petra Ludwig, Madeleine Huber, Matthias Lehr, Marius Wegener, Karolin Zerulla, Christian Lange, Joerg Soppa
Translation initiation in 50-70 % of transcripts in Escherichia coli requires base pairing between the Shine-Dalgarno (SD) motif in the mRNA and the anti-SD motif at the 3' end of the 16S rRNA. However, 30-50 % of E. coli transcripts are non-canonical and are not preceded by an SD motif. The 5' ends of 44 E. coli transcripts were determined, all of which contained a 5'-UTR (no leaderless transcripts), but only a minority contained an SD motif. The 5'-UTR lengths were compared with those listed in RegulonDB and reported in previous publications, and the identities and differences were obtained in all possible combinations...
February 22, 2018: Microbiology
https://www.readbyqxmd.com/read/29469324/-changes-of-liver-fibrosis-related-mirnas-induced-by-soluble-egg-antigen-of-schistosoma-japonicum
#14
Wang Huan, Lu Ya-Jing, Gao Yan-Ru, Wang Shu-Hong, Zhou Rui, Dong Hui-Fen
OBJECTIVE: To investigate the expression of miRNA associated with hepatic fibrosis induced by Schistosoma japonicum soluble egg antigen stimulation in mouse hepatocytes (AML12), so as to lay the foundation for clarifying the mechanism of schistosome infection leading to hepatic fibrosis. METHODS: The expressions of miR-122, miR-182, miR-23b, miR27b and KSRP in AML12 cells treated with SEA were measured by q-PCR. KSRP protein in cell lyses was measured by Western blotting...
March 8, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29469087/downregulation-of-hif-2%C3%AE-reverse-the-chemotherapy-resistance-of-lung-adenocarcinoma-a549-cells-to-cisplatin
#15
Zhao-Jia Gao, Wei-Dong Yuan, Jun-Qiang Yuan, Kai Yuan, Yong Wang
BACKGROUND Cisplatin (DDP)-based systemic chemotherapy has been widely used in the treatment of postoperative or advanced NSCLC patients, however, its effective rate is only 14~40%. HIF-2α can upregulate drug-resistant-related genes expression and lead to chemotherapy resistance in many tumors. However, little is known about the relationship between HIF-2α and chemotherapy resistance of lung cancer cells. MATERIAL AND METHODS In our study, the siRNA expression vectors targeting the HIF-2α gene were designed, constructed, and transfected into A549 cells...
February 22, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29469038/bacterial-translocation-is-linked-to-increased-intestinal-ifn-%C3%AE-il-4-il-17-and-mucin-2-in-cholestatic-rats
#16
Natali Vega-Magaña, Vidal Delgado-Rizo, Leonel García-Benavides, Susana Del Toro-Arreola, Jorge Segura-Ortega, Adelaida Sara M Zepeda Morales, José Sergio Zepeda-Nuño, Marta Escarra-Senmarti, Jorge Gutiérrez-Franco, Jesse Haramati, Miriam R Bueno-Topete
Background and rationale for the study. Bacterial translocation is an important triggering factor of infection and mortality in cirrhosis. In a rat model using bile duct ligation (BDL), bacterial translocation appears within 24 h after ligation. The dynamic between TH1/TH2/TH17 cytokines and the integrity of the colonic mucosa in the context of cirrhosis is little known. This study aims to determine the link between bacterial translocation and intestinal inflammation in a cholestasis model. Additionally, alterations of the colonic mucus layer and the bacterial load were also addressed...
March 1, 2018: Annals of Hepatology
https://www.readbyqxmd.com/read/29468680/proinflammatory-role-of-blister-fluid-derived-exosomes-in-bullous-pemphigoid
#17
Hui Fang, Shuai Shao, Man Jiang, Erle Dang, Shengxian Shen, Jieyu Zhang, Pei Qiao, Caixia Li, Gang Wang
Bullous pemphigoid is an autoimmune inflammatory disorder characterised by the presence of autoantibodies against bullous pemphigoid autoantigens, leading to dermal-epidermal separation with consequent blister formation. However, whether and how the components of blister fluid exacerbate the progression of bullous pemphigoid is unclear. Exosomes are nanometre-sized vesicles released from cells into the body fluid, where they can transmit signals throughout the body. In the present study, we isolated and characterised exosomes from the blister fluids of patients with bullous pemphigoid, evaluated their proinflammatory role, and identified the underlying molecular mechanisms...
February 22, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29468369/role-of-endoplasmic-reticulum-stress-in-12-15-lipoxygenase-induced-retinal-microvascular-dysfunction-in-a-mouse-model-of-diabetic-retinopathy
#18
Khaled Elmasry, Ahmed S Ibrahim, Heba Saleh, Nehal Elsherbiny, Sally Elshafey, Khaled A Hussein, Mohamed Al-Shabrawey
AIMS/HYPOTHESIS: Our earlier studies have established the role of 12/15-lipoxygenase (LO) in mediating the inflammatory reaction in diabetic retinopathy. However, the exact mechanism is still unclear. The goal of the current study was to identify the potential role of endoplasmic reticulum (ER) stress as a major cellular stress response in the 12/15-LO-induced retinal changes in diabetic retinopathy. METHODS: We used in vivo and in vitro approaches. For in vivo studies, experimental diabetes was induced in wild-type (WT) mice and 12/15-Lo (also known as Alox15) knockout mice (12/15-Lo-/- ); ER stress was then evaluated after 12-14 weeks of diabetes...
February 21, 2018: Diabetologia
https://www.readbyqxmd.com/read/29468362/development-of-a-nanogold-slot-blot-inhibition-assay-for-the-detection-of-antibodies-against-bovine-herpesvirus-type-1
#19
Greice Japolla, Jair Pereira Cunha-Junior, Ana Claudia Arantes Marquez Pajuaba, Ernesto Akio Taketomi, Samira Bührer-Sékula, Luiz Artur Mendes Bataus, Guilherme Rocha Lino de Souza
Bovine herpesvirus type 1 (BoHV-1) is recognized as an important pathogen causing respiratory, reproductive, and neurological disorders in cattle and is associated with economic losses to animal industry. Accurate diagnostic methods are needed for prevention of disease transmission. While the virus neutralization test is considered the gold standard method, it requires maintenance of the virus and cell cultures, which is time consuming and expensive. Serological techniques such as enzyme-linked immunosorbent assay (ELISA) are widely applied, as these are easy to perform and provide quick results...
February 21, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29468183/charcot-marie-tooth-disease-type-4j-with-complex-central-nervous-system-features
#20
James P Orengo, Pravin Khemani, John W Day, Jun Li, Carly E Siskind
We describe a family with Charcot Marie Tooth disease type 4J presenting with features of Charcot Marie Tooth disease plus parkinsonism and aphemia. Genetic testing found two variants in the FIG4 gene: c.122T>C (p.I41T) - the most common Charcot Marie Tooth disease type 4J variant - and c.1949-10T>G (intronic). Proband fibroblasts showed absent FIG4 protein on western blot, and skipping of exon 18 by RT-PCR. As most patients with Charcot Marie Tooth disease type 4J do not have central nervous system deficits, we postulate the intronic variant and I41T mutation together are causing loss of FIG4 protein and subsequently the central nervous system findings in our family...
February 2018: Annals of Clinical and Translational Neurology
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