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https://www.readbyqxmd.com/read/28528550/isotachophoretic-fluorescence-in-situ-hybridization-of-intact-bacterial-cells
#1
Sui Ching Phung, Joan Marc Cabot, Mirek Macka, Shane M Powell, Rosanne M Guijt, Michael C Breadmore
A counter-pressure assisted capillary isotachophoresis method in combination with a sieving matrix and ionic spacer was used to perform in-line fluorescence in situ hybridization (FISH) of bacterial cells. A high concentration of sieving matrix (1.8% w/v HEC) was introduced at one end of the capillary and the bacterial cells were suspended in the spacer electrolyte for injection. Using a 2 min injection with 18 psi counter-pressure, 50% of the cells injected into the capillary were hybridized with the fluorescently labelled oligonucleotide and the excess unhybridized probe was separated from the hybridized cells-probe complexes in a two stage ITP method...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28525829/epidemiology-and-management-of-primary-immune-thrombocytopenia-a-nationwide-population-based-study-in-korea
#2
Ji Yun Lee, Ju-Hyun Lee, Heeyoung Lee, Beodeul Kang, Ji-Won Kim, Se Hyun Kim, Jeong-Ok Lee, Jin Won Kim, Yu Jung Kim, Keun-Wook Lee, Jee Hyun Kim, Hyoung Soo Choi, Jong Seok Lee, Soo-Mee Bang
INTRODUCTION: The epidemiology of immune thrombocytopenia (ITP) is not well characterized in an Asian population. MATERIALS AND METHODS: From July 2010 to June 2014, ITP patients were identified using the Korean Health Insurance Review and Assessment Service database. RESULTS: The overall incidence rate of ITP was 5.3 per 100,000 person-years (95% CI: 5.1-5.5). The overall incidence rate ratios of children under 15years old to adults and females to males were 3...
May 11, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28522825/integrated-trophic-position-decreases-in-more-diverse-communities-of-stream-food-webs
#3
Naoto F Ishikawa, Yoshito Chikaraishi, Naohiko Ohkouchi, Aya R Murakami, Ichiro Tayasu, Hiroyuki Togashi, Jun-Ichi Okano, Yoichiro Sakai, Tomoya Iwata, Michio Kondoh, Noboru Okuda
The relationship between biodiversity and ecosystem functioning is an important theme in environmental sciences. We propose a new index for configuration of the biomass pyramid in an ecosystem, named integrated trophic position (iTP). The iTP is defined as a sum of trophic positions (i.e. the average number of steps involved in biomass transfer) of all the animals in a food web integrated by their individual biomass. The observed iTP for stream macroinvertebrates ranged from 2.39 to 2.79 and was negatively correlated with the species density and the Shannon-Wiener diversity index of the local community...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28511637/the-cytokine-polymorphisms-affecting-th1-th2-increase-the-susceptibility-to-and-severity-of-chronic-itp
#4
Noriyuki Takahashi, Takayuki Saitoh, Nanami Gotoh, Yasuhiro Nitta, Lobna Alkebsi, Tetsuhiro Kasamatsu, Yusuke Minato, Akihiko Yokohama, Norifumi Tsukamoto, Hiroshi Handa, Hirokazu Murakami
BACKGROUND: T-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing...
May 16, 2017: BMC Immunology
https://www.readbyqxmd.com/read/28503691/a-rapidly-prototyped-microfluidic-device-for-size-based-nucleic-acid-fractionation-using-isotachophoresis
#5
C Eid, S S Branda, R J Meagher
We present a novel microfluidic device for size-based nucleic acid (NA) fractionation using isotachophoresis (ITP) and an ionic spacer. Our rapid-prototyped laser-cut plastic device has easily modifiable channel dimensions, can process up to 10 μL of sample, and contains an in-line extraction reservoir for minimally-disruptive manual collection of size-fractionated NAs. We designed custom buffering reservoirs using 1 mL pipette tips to provide high buffering capacity and prevent bubbles from entering the microfluidic channels...
May 15, 2017: Analyst
https://www.readbyqxmd.com/read/28500657/modulation-of-herg-channels-by-5-1h-indazol-5-yl-oxy-methyl-n-4-trifluoromethoxy-phenyl-pyrimidin-2-amine-itp-2-a-novel-small-molecule-activator
#6
Harinath Sale, Samrat Roy, Jayakumar Warrier, Srinivasan Thangathirupathy, Yoganand Vadari, Shruthi K Gopal, Prasad Krishnamurthy, Manjunath Ramarao
BACKGROUND AND PURPOSE: hERG channel activators have potential utility in the treatment of acquired and congenital long QT syndrome. In this article, we describe a new hERG activator, 5-(((1H-indazol-5-yl)oxy)methyl)-N-(4-(trifluoromethoxy)phenyl)pyrimidin-2-amine (ITP-2), with a chemical structure distinct from the previously reported compounds. EXPERIMENTAL APPROACH: We investigated the effect of ITP-2 on hERG1a and hERG1a/1b channels expressed heterologously in HEK-293 cells using conventional electrophysiological methods...
May 12, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28496360/epidural-anesthesia-for-labor-and-delivery-in-a-patient-with-may-hegglin-anomaly-a-case-report
#7
Annas Muhammad Muzannar, Mohammed Al Harbi, Raza Rathore, Nasser Tawfeeq, Freddie Wambi, Nasir Mahmood, Sonia Albrechtova, Vassilios Dimitriou
We report a case of May-Hegglin anomaly (MHA) in a woman who had a successful labor and delivery under epidural anesthesia. MHA is an inherited thrombocytopenia easily misdiagnosed as idiopathic (immune) thrombocytopenic purpura (ITP). Early and appropriate diagnosis of MHA during pregnancy is essential for optimal maternal and neonatal delivery outcome. Additionally, it can avoid unnecessary diagnostic studies, such as bone marrow aspiration and biopsy, and even harmful therapies with corticosteroids, immunosuppressive agents, and splenectomy...
2017: Local and Regional Anesthesia
https://www.readbyqxmd.com/read/28493394/determination-of-effective-charges-and-ionic-mobilities-of-polycationic-antimicrobial-peptides-by-capillary-isotachophoresis-and-capillary-zone-electrophoresis
#8
Tereza Tůmová, Lenka Monincová, Ondřej Nešuta, Václav Čeřovský, Václav Kašička
Capillary ITP (CITP) and CZE were applied to the determination of effective charges and ionic mobilities of polycationic antimicrobial peptides (AMPs). Twelve AMPs (deca- to hexadecapeptides) containing 3-7 basic amino acid residues (His, Lys, Arg) at variable positions of peptide chain were investigated. Effective charges of the AMPs were determined from the lengths of their ITP zones, ionic mobilities and molar concentrations, and from the same parameters of the reference compounds. Lengths of the ITP zones of AMPs and reference compounds were obtained from their CITP analyses in cationic mode using leading electrolyte (LE) composed of 10 mM NH4 OH, 40 mM AcOH, pH 4...
May 11, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28473904/from-chronic-immune-thrombocytopenia-to-severe-aplastic-anemia-recent-insights-into-the-evolution-of-eltrombopag
#9
REVIEW
Harinder Gill, Raymond S M Wong, Yok-Lam Kwong
Thrombopoietin (TPO) is the most potent cytokine stimulating thrombopoiesis. Therapy with exogenous TPO is limited by the formation of antibodies cross-reacting with endogenous TPO. Mimetics of TPO are compounds with no antigenic similarity to TPO. Eltrombopag is an orally-active nonpeptide small molecule that binds to the transmembrane portion of the TPO receptor MPL. Initial trials of eltrombopag have centered on immune thrombocytopenia (ITP), which is due to both increased destruction and decreased production of platelets...
May 2017: Therapeutic Advances in Hematology
https://www.readbyqxmd.com/read/28469947/successful-long-term-use-of-eltrombopag-in-a-patient-with-refractory-severe-thrombocytopenia-associated-with-chronic-lymphocytic-leukemia-that-allowed-oral-anticoagulant-treatment-for-severe-cardiomyopathy
#10
Juárez Salcedo Luis Miguel, Gil-Fernández Juan José
Autoimmune cytopenias (AICs) are frequently associated with chronic lymphocytic leukemia (CLL). The most common of these AICs is autoimmune hemolytic anemia (AIHA); the second most is immune thrombocytopenia (ITP). Here, we report on a patient with CLL-associated ITP, with thrombocytopenia refractory to corticosteroids and intravenous immunoglobulins, in which continuous oral treatment with Eltrombopag allowed initiation and maintenance of an oral anticoagulation treatment with Acenocoumarol that was indicated because of a severe arrhythmogenic cardiomyopathy...
2017: Case Reports in Hematology
https://www.readbyqxmd.com/read/28468795/lack-of-detectable-platelet-autoantibodies-is-correlated-with-non-responsiveness-to-rituximab-treatment-in-itp-patients
#11
Leendert Porcelijn, Elly Huiskes, Martin Schipperus, Bronno van der Holt, Masja de Haas, Jaap Jan Zwaginga
No abstract text is available yet for this article.
May 3, 2017: Blood
https://www.readbyqxmd.com/read/28466476/numerical-and-functional-defects-in-cd8-cd28-t-suppressor-lymphocytes-from-patients-with-primary-immune-thrombocytopenia
#12
Huiyuan Li, Yating Hao, Donglei Zhang, Wenjie Liu, Yang Li, Mingen Lyu, Rongfeng Fu, Feng Xue, Xiaofan Liu, Renchi Yang
Primary immune thrombocytopenia (ITP) is an acquired autoimmune disorder, and loss of immune tolerance has been implicated in ITP pathogenesis. CD8(+) CD28(-) suppressor (Ts) cells have an immunosuppression function and are involved in several autoimmune disorders. However, the role of Ts cells in ITP is currently not clear. Here, flow cytometry was used to detect the CD8(+) CD28(-) CD127(-) proportion, which was decreased in active ITP patients compared with that of controls. Function analysis showed that immunosuppression of CD8(+) CD28(-) Ts cells in ITP patients was impaired...
May 3, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28464292/rnai-mediated-gene-silencing-of-itpa-using-a-targeted-nanocarrier-apoptosis-induction-in-skbr3-cancer-cells
#13
Fahimeh Charbgoo, Mehrdad Behmanesh, Maryam Nikkhah, Eric G Kane
A pure nucleotide pool is required for high-fidelity DNA replication and prevention of carcinogenesis in living cells. Human inosine triphosphatase (ITPase), encoded by the ITPA gene, plays a critical role in maintaining the purity of the cellular nucleotide pool by excluding nucleotides that enhance mutagenesis. ITPase is a nucleoside triphosphate pyrophosphatase that hydrolyzes the non-canonical nucleotides inosine triphosphate (ITP) and xanthine triphosphate (XTP). The monophosphate products of ITPase reactions are subsequently excluded from the nucleotide pool and the improper substitution of ITP and XTP into DNA and RNA is prevented...
May 2, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28459549/isotachophoresis-based-surface-immunoassay
#14
Federico Paratore, Tal Zeidman Kalman, Tally Rosenfeld, Govind V Kaigala, Moran Bercovici
In the absence of amplification methods for proteins, the immune-detection of low-abundance proteins using antibodies is fundamentally limited by binding kinetic rates. Here we present a new class of surface-based immunoassays in which protein-antibody reaction is accelerated by isotachophoresis (ITP). We demonstrate the use of ITP to pre-concentrate and deliver target proteins to a surface decorated with specific antibodies, where effective utilization of the focused sample is achieved by modulating the driving electric field (stop-and-diffuse ITP mode) or applying a counter flow that opposes the ITP motion (counterflow ITP mode)...
May 1, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28455894/the-influence-of-early-psychosocial-intervention-on-the-long-term-clinical-outcomes-of-people-suffering-from-schizophrenia
#15
Andrzej Cechnicki, Anna Bielańska
OBJECTIVES: To compare the treatment outcomes of DSM-IV-TR schizophrenia patients in either a Community Treatment Program or an Individual Treatment Program (CTP vs. ITP). The assessment was made after the first hospitalization, and then after three and twelve years. METHODS: Participants were randomly assigned to CTP (experimental) and ITP (traditional) group, with 40 people in each group. 67 people (84%) participated in all three assessments. The socio-demographic and clinical indicators were the same for both groups...
February 26, 2017: Psychiatria Polska
https://www.readbyqxmd.com/read/28454370/methylation-of-cdkn2b-cpg-islands-is-associated-with-upregulated-telomerase-activity-in-children-with-acute-lymphoblastic-leukemia
#16
Qian Hu, Xiaowen Chen, Sixi Liu, Ruiqi Wen, Xiuli Yuan, Dandan Xu, Guosheng Liu, Feiqiu Wen
The aim of the present study was to investigate the association between methylation of cyclin-dependent kinase inhibitor 2B (CDKN2B) CpG islands and telomerase activity in children with acute lymphoblastic leukemia (ALL). A total of 72 children with ALL and 12 children with immune thrombocytopenia (ITP) were subjected to bone marrow aspiration and methylation-specific polymerase chain reaction analysis, and modified telomeric repeat amplification protocol assay analyses, to evaluate CDKN2B methylation and telomerase activity, respectively...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453897/clinical-outcome-of-childhood-chronic-immune-thrombocytopenia-a-38-year-experience-from-a-single-tertiary-center-in-thailand
#17
Thirachit Chotsampancharoen, Pornpun Sripornsawan, Sarapee Duangchoo, Malai Wongchanchailert, Edward McNeil
BACKGROUND: There is limited information on long-term follow-up and prognostic factors for remission among children diagnosed with chronic immune thrombocytopenia (ITP). The aim of this study was to determine clinical outcomes and factors influencing remission in childhood chronic ITP. STUDY DESIGN: The hospital records of children aged 0-15 years diagnosed with chronic ITP were retrospectively reviewed. Kaplan-Meier curves were fit to estimate the median time to complete remission with 95% confidence intervals (CIs)...
April 28, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28444742/the-effect-of-rituximab-on-anti-platelet-autoantibody-levels-in-patients-with-immune-thrombocytopenia
#18
Donald M Arnold, John R Vrbensky, Nadia Karim, James W Smith, Yang Liu, Nikola Ivetic, John G Kelton, Ishac Nazy
Rituximab is an effective therapy resulting in a platelet count improvement in 60% of patients with immune thrombocytopenia (ITP). Rituximab depletes B cells; thus, a reduction in platelet autoantibody levels would be anticipated in patients who achieve a clinical response to this treatment. The objectives of this study were to determine whether rituximab was associated with a reduction in platelet autoantibody levels, and to correlate the loss of autoantibodies with the achievement of a treatment response...
April 25, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28444667/clinical-significance-of-antinuclear-and-antiextractable-nuclear-antigen-antibody-in-childhood-immune-thrombocytopenia
#19
Qihui Liu, Hongzhen Xu, Xianmin Guan, Yali Shen, Xianhao Wen, Yuxia Guo, Jie Yu, Yongchun Su
This study aims to determine the clinical significance of positive antinuclear/antiextractable nuclear antigen (ANA/A-ENA) antibody on manifestation and therapeutic response of childhood immune thrombocytopenia (ITP). Overall, 1,330 patients aged between 1 and 15.6 years diagnosed with primary ITP were retrospectively analyzed, excluding those with secondary ITP. Bleeding manifestations were recorded. All patients underwent autoantibody testing and follow-up for 32 months on average (range: 23-54 months). Steroid response was also assessed...
April 25, 2017: Seminars in Thrombosis and Hemostasis
https://www.readbyqxmd.com/read/28442528/inflammatory-bowel-disease-and-immune-thrombocytopenic-purpura-combined-immune-dysregulation-in-an-adolescent
#20
Karen Queliza, Faith D Ihekweazu, Arshaan Ali, Richard Kellermayer
Concomitant inflammatory bowel disease (IBD) and immune thrombocytopenic purpura (ITP) is a rare phenomenon. A shared immunologic pathway leading to mucosal inflammation and platelet destruction has been proposed. We report a case of a 14-year-old male who presented with abdominal pain, hematochezia, weight loss, and thrombocytopenia. Endoscopic and hematologic evaluations led to the diagnosis of ulcerative colitis (UC) and ITP, respectively. Initial treatment of his UC resulted in improvement in both gastrointestinal symptoms and platelet count...
March 2017: Annals of Clinical and Laboratory Science
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