keyword
MENU ▼
Read by QxMD icon Read
search

OPA

keyword
https://www.readbyqxmd.com/read/28108753/release-of-free-amino-acids-upon-oxidation-of-peptides-and-proteins-by-hydroxyl-radicals
#1
Fobang Liu, Senchao Lai, Haijie Tong, Pascale S J Lakey, Manabu Shiraiwa, Michael G Weller, Ulrich Pöschl, Christopher J Kampf
Hydroxyl radical-induced oxidation of proteins and peptides can lead to the cleavage of the peptide, leading to a release of fragments. Here, we used high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) and pre-column online ortho-phthalaldehyde (OPA) derivatization-based amino acid analysis by HPLC with diode array detection and fluorescence detection to identify and quantify free amino acids released upon oxidation of proteins and peptides by hydroxyl radicals. Bovine serum albumin (BSA), ovalbumin (OVA) as model proteins, and synthetic tripeptides (comprised of varying compositions of the amino acids Gly, Ala, Ser, and Met) were used for reactions with hydroxyl radicals, which were generated by the Fenton reaction of iron ions and hydrogen peroxide...
January 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28088850/the-importance-of-interlayer-equivalent-pores-for-anion-diffusion-in-clay-rich-sedimentary-rocks
#2
Cornelia Wigger, Luc R Van Loon
The anion exclusion behavior in two different clay stones - Opalinus Clay (OPA) and Helvetic Marl (HM) - was studied using a well-established experimental through-diffusion technique. The ionic strength of the pore water was varied between 0.01 M and 5 M to evaluate its effect on the diffusion of HTO and 36Cl-. The total porosity determined by HTO-diffusion was independent of the ionic strength, while the anion accessible porosity varies with the ionic strength of the pore water. In the case of Opalinus Clay the anion accessible porosity increases from 3 % at low ionic strength (0...
January 16, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28079828/vaccination-with-10-valent-pneumococcal-conjugate-vaccine-in-infants-according-to-hiv-status
#3
Shabir A Madhi, Anthonet Koen, Lisa Jose, Nadia van Niekerk, Peter V Adrian, Clare Cutland, Nancy François, Javier Ruiz-Guiñazú, Juan-Pablo Yarzabal, Marta Moreira, Dorota Borys, Lode Schuerman
BACKGROUND: Phase III, open-label, single-center, controlled study in South Africa (ClinicalTrials.gov: NCT00829010) to evaluate immunogenicity, reactogenicity, and safety of the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in human immunodeficiency virus (HIV)-infected (HIV+), HIV-exposed-uninfected (HEU), and HIV-unexposed-uninfected (HUU) children. METHODS: Children stratified by HIV status received PHiD-CV primary vaccination (age 6/10/14 weeks; coadministered with routine childhood vaccines) and booster dose (age 9-10 months)...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28074310/recurrence-of-lower-urinary-tract-symptoms-following-prostate-artery-embolization-for-benign-hyperplasia-single-center-experience-comparing-two-techniques
#4
Francisco Cesar Carnevale, Airton Mota Moreira, Sardis Honoria Harward, Shivank Bhatia, Andre Moreira de Assis, Miguel Srougi, Giovanni Guido Cerri, Alberto Azoubel Antunes
PURPOSE: To compare recurrence of lower urinary tract symptoms (LUTS) recurrence at 12 months following original prostate artery embolization (oPAE) or "proximal embolization first, then embolize distal" (PErFecTED) PAE for benign prostatic hyperplasia (BPH). MATERIALS AND METHODS: 105 consecutive patients older than 45 years, with prostate size greater than 30 cm(3), International Prostate Symptom Score (IPSS) ≥ 8, quality of life (QoL) index ≥ 3, and refractory status or intolerance of medical management were prospectively enrolled between June 2008 and August 2013...
January 10, 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28066100/oropharyngeal-aspiration-of-bleomycin-an-alternative-experimental-model-of-pulmonary-fibrosis-developed-in-swiss-mice
#5
Swarna Bale, Manoj Sunkoju, Shiva Shankar Reddy, Veerabhadra Swamy, Chandraiah Godugu
OBJECTIVE: Pulmonary fibrosis (PF) is a progressive and predominantly lethal form of several interstitial lung diseases with limited current therapeutics; it is, therefore, essential to develop a simple, homogeneous, and noninvasive disease model to investigate possible anti-fibrotic approaches. The present study is designed to develop oropharyngeal aspiration (OPA) model of bleomycin (BLM)-induced PF as a simple and alternative to intratracheal (IT) administration of BLM in Swiss mice strain...
November 2016: Indian Journal of Pharmacology
https://www.readbyqxmd.com/read/28060497/solid-phase-speciation-and-solubility-of-phosphorus-in-an-acid-sulfate-paddy-soil-during-soil-reduction-and-reoxidation-as-affected-by-oil-palm-ash-and-biochar
#6
Worachart Wisawapipat, Kamolchanok Charoensri, Jirawat Runglerttrakoolchai
Understanding phosphorus (P) speciation and how redox conditions control P solubility in acid sulfate paddy soils with limited P availability is crucial for improving soil P availability. We examined P speciation and extractability in an acid sulfate paddy soil incorporated with oil palm ash (OPA) and biochar (OPB) during soil reduction and subsequent oxidation. Phosphorus K-edge X-ray absorption near edge structure (XANES) spectra of the soil samples revealed that P in the soil mainly occurred as P adsorbed to ferrihydrite and P adsorbed to gibbsite...
January 6, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28059345/spectrum-regulation-for-mid-infrared-ultrafast-pulses-via-a-time-synchronization-aperiodically-poled-linbo-sub-3-sub
#7
Haizhe Zhong, Fen Qin, Jianlong Yang, Lifu Zhang, Changwen Xu, Ying Li, Dianyuan Fan
Restricted to temporal separation during the coupled-waves interaction, aperiodically quasi-phase-matching (QPM) nonlinear crystals are primarily implemented for prechirped pulses, showing limited applications in ultrafast temporal scale. Under the proposed time-synchronization framework, pump and signal waves travel with identical group-velocity, which permits sustaining energy transfer in long aperiodically poled LiNbO<sub>3</sub> crystals (APPLN) even with ultrafast pulse duration. With the help of this structure, adiabatic frequency conversion shows extra advantages compared with the common cases, which enables lower stretching ratio and smoother gain spectrum...
December 26, 2016: Optics Express
https://www.readbyqxmd.com/read/28045939/association-of-occupational-and-leisure-time-physical-activity-with-aerobic-capacity-in-a-working-population
#8
Jonas Mundwiler, Ulla Schüpbach, Thomas Dieterle, Jörg Daniel Leuppi, Arno Schmidt-Trucksäss, David Paul Wolfer, David Miedinger, Stefanie Brighenti-Zogg
INTRODUCTION: Objective data on the association of maximal aerobic capacity (VO2max) with work related physical activity are sparse. Thus, it is not clear whether occupational physical activity (OPA) contributes to an increase of VO2max. This study examined the association of VO2max with work and non-work related physical activity in a Swiss working population. METHODS: In this cross-sectional study, a total of 337 healthy and full-time employed adults were recruited...
2017: PloS One
https://www.readbyqxmd.com/read/28043778/neuritogenic-activity-of-bi-functional-bis-tryptoline-triazole
#9
Jutamas Jiaranaikulwanitch, Sarin Tadtong, Piyarat Govitrapong, Valery V Fokin, Opa Vajragupta
Alzheimer's disease (AD) is a common neurodegenerative disorder, one of the hallmarks of which is the deposition of aggregated β-amyloid peptides (Aβ40,42) as plaques in the brain. Oligomers of these peptides have been reported to be toxic and to inhibit neurite outgrowth, as evidenced by neurite dystrophy and significant loss of synaptic connectivity of neurons in the AD brain resulting in cognitive decline. These peptides also react with biological metal in the brain to generate free radicals, thereby aggravating neuronal cell injury and death...
December 23, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28027283/divergent-memory-b-cell-responses-in-a-mixed-infant-pneumococcal-conjugate-vaccine-schedule
#10
Johannes Trück, Ruth Mitchell, Sena Jawad, Elizabeth A Clutterbuck, Matthew D Snape, Dominic F Kelly, Merryn Voysey, Andrew J Pollard
BACKGROUND: Vaccine-induced immunity against pneumococcal infection relies on the generation of high concentrations of antibody and B-cell memory. Both the 10- and the 13-valent pneumococcal conjugate vaccines (PCV-10 and PCV-13) effectively reduce disease caused by vaccine serotypes. It is unknown whether the generation of B-cell memory requires several doses of the same vaccine or whether different PCVs are interchangeable. METHODS: Children in the UK (n=178) who had previously received PCV-13 at 2 and 4 months were randomized 1:1 to receive a PCV-13 or PCV-10 booster at age 12 months...
December 23, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28009403/ocular-pulse-amplitude-after-panretinal-photocoagulation-in-normotensive-eyes-with-proliferative-diabetic-retinopathy
#11
Marija M Bozic, Jelena B Karadzic, Igor M Kovacevic, Ivan S Marjanovic
PURPOSE: To assess the effect of panretinal laser photocoagulation on ocular pulse amplitude (OPA) in normotensive eyes with proliferative diabetic retinopathy. METHODS: Prospectively, we performed unilateral argon laser panretinal photocoagulation (PRP) in 30 patients with diabetes mellitus type II and previously untreated bilateral proliferative diabetic retinopathy. Before and 7 and 30 days after the treatment, OPA was measured using dynamic contour tonometer...
November 28, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/27991937/silver-ions-as-em-marker-of-congo-red-ligation-sites-in-amyloids-and-amyloid-like-aggregates
#12
Janina Rybarska, Leszek Konieczny, Anna Jagusiak, Katarzyna Chłopaś, Grzegorz Zemanek, Barbara Piekarska, Barabara Stopa, Piotr Piwowar, Olga Woźnicka, Irena Roterman
Congo red (CR) is a known selective amyloid ligand. The focus of our work is identification (by EM imaging) of dye binding sites and their distribution in amyloids and amyloid-like aggregates formed in vitro. In order to produce the required contrast, CR has been indirectly combined with metal via including Titan yellow (TY) by intercalation which exhibits a relatively strong affinity for silver ions. The resulting combined ligand retains its ability to bind to proteins (which it owes to CR) and can easily be detected in EM studies thanks to TY...
December 19, 2016: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/27974397/assignment-of-opsonic-values-to-pneumococcal-reference-serum-007sp-for-use-in-opsonophagocytic-assays-for-13-serotypes
#13
R L Burton, J Antonello, D Cooper, D Goldblatt, K H Kim, B D Plikaytis, L Roalfe, D Wauters, F Williams, G L Xie, M H Nahm, M Akkoyunlu
Opsonophagocytic assays (OPAs) are routinely used for assessing the immunogenicity of pneumococcal vaccines, with OPA data often utilized for licensure of new vaccine formulations. However, no reference serum for pneumococcal OPAs is available, making evaluation of data among different laboratories difficult. This international collaboration was initiated to: 1) assign consensus opsonic indexes (OIs) to Pneumococcal Reference Serum Lot 007sp ("007sp") and a panel of calibration sera; and 2) determine if normalization with 007sp decreases the OPA variability among laboratories...
December 14, 2016: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/27960627/comparative-evaluation-of-a-newly-developed-13-valent-pneumococcal-conjugate-vaccine-in-a-mouse-model
#14
Chulmin Park, Eun-Young Kwon, Su-Mi Choi, Sung-Yeon Cho, Ji-Hyun Byun, Jung Yeon Park, Dong-Gun Lee, Jin Han Kang, Jinhwan Shin, Hun Kim
Animal models facilitate evaluation of vaccine efficacy at relatively low cost. This study was a comparative evaluation of the immunogenicity and protective efficacy of a new 13-valent pneumococcal conjugate vaccine (PCV13) with a control vaccine in a mouse model. After vaccination, anti-capsular antibody levels were evaluated by pneumococcal polysaccharide (PnP) enzyme-linked immunosorbent assay (ELISA) and opsonophagocytic killing assay (OPA). Also, mice were challenged intraperitoneally with 100-fold of the 50% lethal dose of Streptococcus pneumoniae...
December 14, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27934268/volatile-organic-compound-emissions-from-humans-indoors
#15
Xiaochen Tang, Pawel K Misztal, William W Nazaroff, Allen H Goldstein
Research on the sources of indoor airborne chemicals has traditionally focused on outdoor air, building materials, furnishings, and activities such as smoking, cooking, and cleaning. Relatively little research has examined the direct role of occupant emissions, even though this source clearly contributes to indoor volatile organic compounds (VOCs) and influences indoor chemistry. In this work, we quantify occupant-related gaseous VOC emissions in a university classroom using a proton-transfer-reaction time-of-flight mass spectrometer...
December 6, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27919966/multimodal-primary-treatment-of-metastatic-prostate-cancer-with-androgen-deprivation-and-radiation
#16
Timo Joensuu, Greetta Joensuu, Kalevi Kairemo, Timo Kiljunen, Maigo Riener, Aili Aaltonen, Martti Ala-Opas, Aki Kangasmäki, Tuomo Alanko, Lauri Taipale, Petteri Hervonen, Anna Bützow, Irene Virgolini, Akseli Hemminki
AIM: We combined anti-androgen therapy with radiotherapy in a first-line setting for metastatic prostate cancer aiming to cause maximal cancer-cell death to delay the emergence of castration-resistant disease. MATERIALS AND METHODS: In this non-randomized retrospective series of 46 patients, the initial median prostate-specific antigen (PSA) was 98.5 μg/l (range=6.7-15,500), median Gleason score 9 and most men had at least T3N1M1 disease. All patients received luteinizing hormone releasing hormone analog or degarelix with bicalutamide...
2016: Anticancer Research
https://www.readbyqxmd.com/read/27919632/immunogenicity-and-safety-of-a-13-valent-pneumococcal-conjugate-vaccine-and-an-mf59-adjuvanted-influenza-vaccine-after-concomitant-vaccination-in-%C3%A2-60-year-old-adults
#17
Joon Young Song, Hee Jin Cheong, Hak Jun Hyun, Yu Bin Seo, Jacob Lee, Seong-Heon Wie, Min Joo Choi, Won Suk Choi, Ji Yun Noh, Jae Won Yun, Jin Gu Yun, Woo Joo Kim
BACKGROUND: Concomitant administration of influenza and pneumococcal vaccines could be an efficient strategy to increase vaccine uptake among older adults. Nevertheless, immune interference and safety issues have been a concern when more than one vaccines are administered at the same time. METHODS: Subjects aged ⩾60years were randomized in a 1:1:1 ratio to receive MF59-adjuvanted trivalent inactivated influenza vaccine (MF59-aTIV)+13-valent pneumococcal conjugate vaccine (PCV13) (Group 1), PCV13 alone (Group 2), or MF59-aTIV alone (Group 3)...
January 5, 2017: Vaccine
https://www.readbyqxmd.com/read/27916718/loss-of-the-opa-interacting-protein-5-inhibits-breast-cancer-proliferation-through-mir-139-5p-notch1-pathway
#18
Hong-Chang Li, Ya-Feng Chen, Wen Feng, Han Cai, Yi Mei, Yi-Ming Jiang, Teng Chen, Ke Xu, Dian-Xu Feng
Opa interacting protein 5 (OIP5) has been reported to be over-expressed in several kinds of human cancer. However, the biological function and clinical significance of OIP5 in human breast cancer remains unknown. In this study, we found that OIP5 was notably over-expressed in breast cancer tissues compared with their corresponding nontumorous tissues. Statistical analysis showed a significant correlation of OIP5 expression with advanced clinical stage. Ablation OIP5 inhibited the proliferation of breast cancer cells...
March 1, 2017: Gene
https://www.readbyqxmd.com/read/27916408/safety-tolerability-and-immunogenicity-of-a-4-antigen-staphylococcus-aureus-vaccine-sa4ag-results-from-a-first-in-human-randomised-placebo-controlled-phase-1-2-study
#19
Robert W Frenck, C Buddy Creech, Eric A Sheldon, David J Seiden, Martin K Kankam, James Baber, Edward Zito, Robin Hubler, Joseph Eiden, Joseph M Severs, Shite Sebastian, Jasdeep Nanra, Kathrin U Jansen, William C Gruber, Annaliesa S Anderson, Douglas Girgenti
BACKGROUND: A prophylactic Staphylococcus aureus four-antigen vaccine (SA4Ag) is under development for prevention of invasive S. aureus disease. A preliminary S. aureus three-antigen vaccine (SA3Ag) was reformulated to include a novel manganese transporter protein (MntC or rP305A). This study describes the first-in-human dose-finding, safety, and immunogenicity results for SA4Ag. METHODS: In this double-blind, sponsor-unblind, placebo-controlled, phase 1/2 study, 454 healthy adults aged 18-64years were randomised to receive a single dose of one of three formulations of SA4Ag with escalating dose levels of rP305A or placebo...
January 5, 2017: Vaccine
https://www.readbyqxmd.com/read/27897327/high-performance-liquid-chromatography-evaluation-of-the-enantiomeric-purity-of-amino-acids-by-means-of-automated-precolumn-derivatization-with-ortho-phthalaldehyde-and-chiral-thiols
#20
Raphael Kühnreich, Ulrike Holzgrabe
The use of ortho-phthalaldehyde (OPA) for the derivatization of amino acids (AA) is well known. It enables the separation of the derivatives on common reversed phase columns and improves the sensitivity with fluorescence detection. With the use of a chiral thiol an indirect enantioseparation of chiral amines and AAs is feasible. The major drawback of the OPA-derivatization is the poor stability of the products. Here, a method with an in-needle derivatization procedure is optimized to facilitate a quantitative conversion of the AA with OPA and the chiral thiols N-acetyl-L-cysteine or N-isobutyryl-L-cysteine, followed by a subsequent analysis, eluding the stability issue...
December 2016: Chirality
keyword
keyword
81685
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"