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https://www.readbyqxmd.com/read/28811020/peanut-agglutinin-and-%C3%AE-cyclodextrin-functionalized-polymer-monolith-microextraction-of-igg-galactosylation-coupled-with-online-ms-detection
#1
Haijiao Zheng, Tenggao Zhu, Xiqian Li, Jiutong Ma, Qiong Jia
A facile online method coupling polymer monolithic microextraction (PMME) with mass spectrometry (MS) was developed for the detection of Immunoglobulin G (IgG) galactosylation glycopeptides. A peanut agglutinin-β-cyclodextrin (PNA-β-CD) functionalized poly(hydroxyethyl methylacrylate-ethyleneglycol dimethacrylate) monolith was designed via a click reaction. Thanking to the specificity of PNA-β-CD for the targets, the material exhibited enhanced enrichment selectivity and extraction efficiency for IgG galactosylation glycopeptides...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28809959/expression-and-activity-of-angiotensin-regulating-enzymes-is-associated-with-prognostic-outcome-in-clear-cell-renal-cell-carcinoma-patients
#2
Peio Errarte, Maider Beitia, Itxaro Perez, Lorea Manterola, Charles H Lawrie, Jon Danel Solano-Iturri, Julio Calvete-Candenas, Miguel Unda, José I López, Gorka Larrinaga
The discovery of the intrarenal renin-angiotensin system (iRAS), which regulates angiogenesis, cell differentiation and proliferation, has opened new perspectives in the knowledge of kidney carcinogenesis. In this study we analyzed the immunohistochemical expression and fluorimetric activity of four key peptidases of iRAS in tumor tissue (n = 144) and serum samples (n = 128) from patients with renal neoplasms. Neutral endopeptidase (NEP/CD10), Angiotensin-converting enzyme-2 (ACE2), and aminopeptidase A (APA) were expressed in tumor cells whilst Angiotensin-converting enzyme (ACE) was expressed in the endothelial cells of intratumor blood vessels...
2017: PloS One
https://www.readbyqxmd.com/read/28807995/regulation-of-the-cell-cycle-and-inflammatory-arthritis-by-the-transcription-cofactor-lbh-gene
#3
Shinji Matsuda, Deepa Hammaker, Katharyn Topolewski, Karoline J Briegel, David L Boyle, Steven Dowdy, Wei Wang, Gary S Firestein
Rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) display unique aggressive behavior, invading the articular cartilage and promoting inflammation. Using an integrative analysis of RA risk alleles, the transcriptome and methylome in RA FLS, we recently identified the limb bud and heart development (LBH) gene as a key dysregulated gene in RA and other autoimmune diseases. Although some evidence suggests that LBH could modulate the cell cycle, the precise mechanism is unknown and its impact on inflammation in vivo has not been defined...
August 14, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28805898/ability-of-garlic-derived-diallyl-disulfide-and-diallyl-trisulfide-supplemented-by-oral-gavage-to-mitigate-effects-of-an-acute-postweaning-feed-and-water-deprivation-event-in-nursery-pigs
#4
N Horn, G Miller, K M Ajuwon, O Adeola
Compounds in garlic have been shown to contain anti-inflammatory, antioxidant, and immune modulatory properties that may be able to mitigate the effects of nursery pig stressors. The objective of the current experiment was to determine if oral gavage of garlic-derived diallyl disulfide (DADS) and diallyl trisulfide (DATS) could mitigate the effects of a 24-h postweaning feed + water deprivation event in nursery pigs. Pigs (6.0 ± 0.05 kg and 21 d old) were allotted to 4 treatments in a randomized complete block design at weaning with 8 replicate pens per treatment that consisted of with or without a 24-h postweaning feed + water deprivation event and with or without an oral gavage containing 3...
August 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28804537/an-integrated-approach-to-coronary-heart-disease-diagnosis-and-clinical-management
#5
REVIEW
Teresa Infante, Ernesto Forte, Concetta Schiano, Carlo Cavaliere, Carlo Tedeschi, Andrea Soricelli, Marco Salvatore, Claudio Napoli
The major issue in coronary heart disease (CHD) diagnosis and management is that symptoms onset in an advanced state of disease. Despite the availability of several clinical risk scores, the prediction of cardiovascular events is lacking, and many patients at risk are not well stratified according to the canonical risk factors alone. Therefore, adequate risk assessment remains the most challenging issue. Recently, the integration of imaging data with biochemical markers in a radiogenomic framework has been proposed in many fields of medicine as well as in cardiology...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28799653/kindlin-2-interacts-with-endothelial-adherens-junctions-to-support-vascular-barrier-integrity
#6
Elzbieta Pluskota, Kamila M Bledzka, Katarzyna Bialkowska, Dorota Szpak, Dmitry A Soloviev, Sidney Jones, Dmitriy Verbovetskiy, Edward F Plow
Endothelial cells (EC) establish a physical barrier between the blood and surrounding tissue. Impairment of this barrier can occur during inflammation, ischemia or sepsis and cause severe organ dysfunction. Kindlin-2, primarily recognized as a focal adhesion protein in EC, was not anticipated to have a role in vascular barrier. We tested the role of Kindlin-2 in regulating vascular integrity using several different approaches to decrease Kindlin-2 levels in EC. Reduced levels of Kindlin-2 in Kindlin-2(+/-) mice, aortic endothelial cells (MAECs) from these(-) mice, and human umbilical ECs (HUVEC) treated with Kindlin-2 siRNA showed enhanced basal and Platelet Activating Factor (PAF) or lipopolysaccharide-stimulated vascular leakage compared to wild-type (WT) counterparts...
August 11, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28797776/increase-in-seizure-susceptibility-in-sepsis-like-condition-explained-by-spiking-cytokines-and-altered-adhesion-molecules-level-with-impaired-blood-brain-barrier-integrity-in-experimental-model-of-rats-treated-with-lipopolysaccharides
#7
Rakesh K Sewal, Manish Modi, Uma Nahar Saikia, Amitava Chakrabarti, Bikash Medhi
BACKGROUND AND OBJECTIVE: Epilepsy is a neurological disorder characterized by recurrent unprovoked seizures. Sepsis is a condition which initiates a cascade of a surge of inflammatory mediators. Interplay between seizures and inflammation other than of brain origin is yet to be explored. The present study was designed to evaluate the seizure susceptibility in experimental models of lipopolysaccharide (LPS) induced sepsis. DESIGN AND METHODS: Experimental sepsis was induced using lipopolysaccharides in Wistar rats...
June 15, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/28797112/a-novel-whole-bacterial-enzyme-linked-immunosorbant-assay-to-quantify-chlamydia-trachomatis-specific-antibodies-reveals-distinct-differences-between-systemic-and-genital-compartments
#8
Hannah L Albritton, Pamela A Kozlowski, Rebecca A Lillis, Chris L McGowin, Julia D Siren, Stephanie N Taylor, Joyce A Ibana, Lyndsey R Buckner, Li Shen, Alison J Quayle
Chlamydia trachomatis (CT) is the leading sexually transmitted bacterial infection. The continued global burden of CT infection strongly predicates the need for a vaccine to supplement current chlamydial control programs. The correlates of protection against CT are currently unknown, but they must be carefully defined to guide vaccine design. The localized nature of chlamydial infection in columnar epithelial cells of the genital tract necessitates investigation of immunity at the site of infection. The purpose of this study was to develop a sensitive whole bacterial enzyme-linked immunosorbent assay (ELISA) to quantify and compare CT-specific IgG and IgA in sera and genital secretions from CT-infected women...
2017: PloS One
https://www.readbyqxmd.com/read/28794899/an-extended-model-for-culture-dependent-heterogenous-gene-expression-and-proliferation-dynamics-in-mouse-embryonic-stem-cells
#9
Simon Godwin, Daniel Ward, Elisa Pedone, Martin Homer, Alexander G Fletcher, Lucia Marucci
During development, pluripotency is a transient state describing a cell's ability to give rise to all three germ layers and germline. Recent studies have shown that, in vitro, pluripotency is highly dynamic: exogenous stimuli provided to cultures of mouse embryonic stem cells, isolated from pre-implantation blastocysts, significantly affect the spectrum of pluripotency. 2i/LIF, a recently defined serum-free medium, forces mouse embryonic stem cells into a ground-state of pluripotency, while serum/LIF cultures promote the co-existence of ground-like and primed-like mouse embryonic stem cell subpopulations...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28794205/the-human-serum-metabolome-of-vitamin-b-12-deficiency-and-repletion-and-associations-with-neurological-function-in-elderly-adults
#10
Alex Brito, Dmitry Grapov, Johannes Fahrmann, Danielle Harvey, Ralph Green, Joshua W Miller, Sergey N Fedosov, Setareh Shahab-Ferdows, Daniela Hampel, Theresa L Pedersen, Oliver Fiehn, John W Newman, Ricardo Uauy, Lindsay H Allen
Background: The specific metabolomic perturbations that occur in vitamin B-12 deficiency, and their associations with neurological function, are not well characterized.Objective: We sought to characterize the human serum metabolome in subclinical vitamin B-12 deficiency and repletion.Methods: A before-and-after treatment study provided 1 injection of 10 mg vitamin B-12 (with 100 mg pyridoxine and 100 mg thiamin) to 27 community-dwelling elderly Chileans (∼74 y old) with vitamin B-12 deficiency, as evaluated with serum vitamin B-12, total plasma homocysteine (tHcy), methylmalonic acid (MMA), and holotranscobalamin...
August 9, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28793850/glycation-oxidation-and-glycoxidation-of-igg-a-biophysical-biochemical-immunological-and-hematological-study
#11
Sidra Islam, Moinuddin, Abdul Rouf Mir, Alok Raghav, Safia Habib, Khursheed Alam, Asif Ali
Glycation and oxidation induce structural alterations in the proteins in an inter-dependent manner with consequent pathological implications. The published literature presents wide range of modifications in conformational characteristics of proteins by glycation and oxidation; however, there is little data that could elaborate the cumulative effect of both the processes. This study has analysed the modifications in IgG by methylglyoxal (MG) (glycative stress), hydroxyl radical (OH(•)) (oxidative stress) and by their combined action i...
August 9, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28792511/aldehyde-dehydrogenase-1-aldh1-isoform-expression-and-potential-clinical-implications-in-hepatocellular-carcinoma
#12
Cheng-Kun Yang, Xiang-Kun Wang, Xi-Wen Liao, Chuang-Ye Han, Ting-Dong Yu, Wei Qin, Guang-Zhi Zhu, Hao Su, Long Yu, Xiao-Guang Liu, Si-Cong Lu, Zhi-Wei Chen, Zhen Liu, Ke-Tuan Huang, Zheng-Tao Liu, Yu Liang, Jian-Lu Huang, Kai-Yin Xiao, Min-Hao Peng, Cheryl Ann Winkle, Stephen J O'Brien, Tao Peng
Hepatocellular carcinoma (HCC) is one of the most prevalent and life-threatening malignancies worldwide. There are few diagnostic and prognostic biomarkers and druggable targets for HCC. Aldehyde dehydrogenase 1 (ALDH1) is a marker of stem cells in a variety of cancers, but the mRNA levels and prognostic value of ALDH1 isoforms in HCC patients remain unknown. In the present study, gene ontology annotation of the ALDH1 family was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID), and the gene pathway analsis was performed using GeneMANIA software...
2017: PloS One
https://www.readbyqxmd.com/read/28791745/a-novel-pathway-of-protein-haptenation-by-%C3%A3-lactams
#13
M Consuelo Jiménez, Raúl Pérez-Ruiz, Emilio Lence, Inmaculada Andreu, Concepción González-Bello, Miguel A Miranda, Daniel Limones-Herrero
The covalent binding of β-lactams to proteins upon photochemical activation has been demonstrated using an integrated approach that combines photochemical, proteomic and computational studies, selecting human serum albumin (HSA) as target protein and ezetimibe (1) as probe. The results have revealed a novel protein haptenation pathway by this family of drugs that is alternative to the known nucleophilic ring opening of β-lactams by the free amino group of lysine residues. Thus, photochemical ring splitting of the β-lactam ring following a formal retro-Staudinger reaction gives a highly reactive ketene intermediate that is trapped by the neighboring lysine residues, leading to an amide adduct...
August 9, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28783713/aging-and-sarcopenia-associate-with-specific-interactions-between-gut-microbes-serum-biomarkers-and-host-physiology-in-rats
#14
Jay Siddharth, Anirikh Chakrabarti, Alice Pannérec, Sonia Karaz, Delphine Morin-Rivron, Mojgan Masoodi, Jerome N Feige, Scott James Parkinson
The microbiome has been demonstrated to play an integral role in the maintenance of many aspects of health that are also associated with aging. In order to identify areas of potential exploration and intervention, we simultaneously characterized age-related alterations in gut microbiome, muscle physiology and serum proteomic and lipidomic profiles in aged rats to define an integrated signature of the aging phenotype. We demonstrate that aging skews the composition of the gut microbiome, in particular by altering the Sutterella to Barneseilla ratio, and alters the metabolic potential of intestinal bacteria...
July 17, 2017: Aging
https://www.readbyqxmd.com/read/28778061/studies-on-the-interaction-of-polylactid-based-planar-and-nanoparticular-biomaterials-with-serum-albumin-and-fibrinogen
#15
Katharina Wulf, Ulrike Tschierschky, Thomas Eickner, Niels Grabow, Ralf G Mundkowski
Knowledge about the interactions of biomaterials with biological matrices is still poor however crucial to future development in terms of how to cope with immunomodulatory adverse effects and to control the integration of implants into tissue or the targeting of nano devices. The appropriate design of a material depends on reliable data about the influence of oligomer or polymer configuration and dispersity as well as surface properties including chemical modifications. This work addressed the interaction of four polylactide (PLA) resomers, a well-established, biodegradable biomaterial, and two physiologically relevant proteins, which are ubiquitous in human serum - albumin (HSA) and fibrinogen (HFG)...
July 8, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28774952/vitamin-d-supplementation-of-initially-vitamin-d-deficient-mice-diminishes-lung-inflammation-with-limited-effects-on-pulmonary-epithelial-integrity
#16
Shelley Gorman, Alysia G Buckley, Kak-Ming Ling, Luke J Berry, Vanessa S Fear, Stephen M Stick, Alexander N Larcombe, Anthony Kicic, Prue H Hart
In disease settings, vitamin D may be important for maintaining optimal lung epithelial integrity and suppressing inflammation, but less is known of its effects prior to disease onset. Female BALB/c dams were fed a vitamin D3-supplemented (2280 IU/kg, VitD(+)) or nonsupplemented (0 IU/kg, VitD(-)) diet from 3 weeks of age, and mated at 8 weeks of age. Male offspring were fed the same diet as their mother. Some offspring initially fed the VitD(-) diet were switched to a VitD(+) diet from 8 weeks of age (VitD(-/+))...
August 2017: Physiological Reports
https://www.readbyqxmd.com/read/28771604/role-of-glucocorticoid-induced-leucine-zipper-gilz-in-inflammatory-bone-loss
#17
Nianlan Yang, Babak Baban, Carlos M Isales, Xing-Ming Shi
TNF-α plays a key role in the development of rheumatoid arthritis (RA) and inflammatory bone loss. Unfortunately, treatment of RA with anti-inflammatory glucocorticoids (GCs) also causes bone loss resulting in osteoporosis. Our previous studies showed that overexpression of glucocorticoid-induced leucine zipper (GILZ), a mediator of GC's anti-inflammatory effect, can enhance osteogenic differentiation in vitro and bone acquisition in vivo. To investigate whether GILZ could antagonize TNF-α-induced arthritic inflammation and protect bone in mice, we generated a TNF-α-GILZ double transgenic mouse line (TNF-GILZ Tg) by crossbreeding a TNF-α Tg mouse, which ubiquitously expresses human TNF-α, with a GILZ Tg mouse, which expresses mouse GILZ under the control of a 3...
2017: PloS One
https://www.readbyqxmd.com/read/28770611/clinical-significance-of-mirna-autophagy-transcript-expression-in-patients-with-hepatocellular-carcinoma
#18
Marwa Matboli, Ayman E Shafei, Hanan H Shehata, Nesreen Nabil, Nourhan Hossam, Ahmed Em Azazy, Ahmed Hf El-Tawdi, Omar Abdel-Rahman
AIM: This study integrates autophagy transcripts miRNAs expression based on bioinformatic analysis followed by clinical validation. METHODOLOGY: Cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a level were investigated in the serum and tissue of patients with hepatocellular carcinoma (HCC), chronic hepatitis C and healthy volunteers by quantitative real-time PCR. The prognostic power of this serum RNA panel was explored. RESULTS: The expression of serum cellular jun proto-oncogene mRNA, LAMP2 mRNA, miR-16 and miR-146a were positive in 85...
August 3, 2017: Biomarkers in Medicine
https://www.readbyqxmd.com/read/28769926/serum-neuroinflammatory-disease-induced-central-nervous-system-proteins-predict-clinical-onset-of-experimental-autoimmune-encephalomyelitis
#19
Itay Raphael, Johanna Webb, Francisco Gomez-Rivera, Carol A Chase Huizar, Rishein Gupta, Bernard P Arulanandam, Yufeng Wang, William E Haskins, Thomas G Forsthuber
There is an urgent need in multiple sclerosis (MS) patients to develop biomarkers and laboratory tests to improve early diagnosis, predict clinical relapses, and optimize treatment responses. In healthy individuals, the transport of proteins across the blood-brain barrier (BBB) is tightly regulated, whereas, in MS, central nervous system (CNS) inflammation results in damage to neuronal tissues, disruption of BBB integrity, and potential release of neuroinflammatory disease-induced CNS proteins (NDICPs) into CSF and serum...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28769765/mesenchymal-stem-cell-derived-extracellular-vesicles-ameliorates-hippocampal-synaptic-impairment-after-transient-global-ischemia
#20
Mingyang Deng, Han Xiao, Hainan Zhang, Hongling Peng, Huan Yuan, Yunxiao Xu, Guangsen Zhang, Zhiping Hu
Recent studies have found that administration of stem cells or extracellular vehicles (EVs) derived from stem cells exert neuroprotective effects after transient global ischemia. However, the underlying mechanisms of this effect remain unclear, especially at the level of synaptic functions. In this study, we compared the suppressive effects on cyclooxygenase-2 (COX-2) upregulation by EVs derived from bone marrow mesenchymal stem cells (BMSC-EV), adipose tissue MSC (AdMSC-EV) and serum (serum-EV). Then we examined whether BMSC-EVs could restore functional integrity of synaptic transmission and plasticity...
2017: Frontiers in Cellular Neuroscience
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