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https://www.readbyqxmd.com/read/28423310/c-myb-regulates-the-t-bet-dependent-differentiation-program-in-b-cells-to-coordinate-antibody-responses
#1
Dana Piovesan, Jessica Tempany, Andrea Di Pietro, Inge Baas, Callisthenis Yiannis, Kristy O'Donnell, Yunshun Chen, Victor Peperzak, Gabrielle T Belz, Charles R Mackay, Gordon K Smyth, Joanna R Groom, David M Tarlinton, Kim L Good-Jacobson
Humoral immune responses are tailored to the invading pathogen through regulation of key transcription factors and their networks. This is critical to establishing effective antibody-mediated responses, yet it is unknown how B cells integrate pathogen-induced signals to drive or suppress transcriptional programs specialized for each class of pathogen. Here, we detail the key role of the transcription factor c-Myb in regulating the T-bet-mediated anti-viral program. Deletion of c-Myb in mature B cells significantly increased serum IgG2c and CXCR3 expression by upregulating T-bet, normally suppressed during Th2-cell-mediated responses...
April 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28422991/mortality-is-associated-with-inflammation-anemia-specific-diseases-and-treatments-and-molecular-markers
#2
Mark Moeller, Christiane Pink, Nicole Endlich, Karlhans Endlich, Hans-Jörgen Grabe, Henry Völzke, Marcus Dörr, Matthias Nauck, Markus M Lerch, Rüdiger Köhling, Birte Holtfreter, Thomas Kocher, Georg Fuellen
Lifespan is a complex trait, and longitudinal data for humans are naturally scarce. We report the results of Cox regression and Pearson correlation analyses using data of the Study of Health in Pomerania (SHIP), with mortality data of 1518 participants (113 of which died), over a time span of more than 10 years. We found that in the Cox regression model based on the Bayesian information criterion, apart from chronological age of the participant, six baseline variables were considerably associated with higher mortality rates: smoking, mean attachment loss (i...
2017: PloS One
https://www.readbyqxmd.com/read/28422412/histological-evolution-of-bk-virus-associated-nephropathy-importance-of-integrating-clinical-and-pathological-findings
#3
Cinthia B Drachenberg, John C Papadimitriou, Muhammad R Chaudhry, Richard Ugarte, Manju Mavanur, Beje Thomas, Charles Cangro, Nadiesda Costa, Emilio Ramos, Matthew R Weir, Abdolreza Haririan
Long term clinicopathological studies of BK-associated nephropathy (PyVAN) are not available. We studied 206 biopsies (71 patients), followed 3.09±1.46y after immunosuppression reduction. The biopsy features (% SV40+staining and inflammation +/- acute rejection) were correlated with viral load dynamics and serum creatinine to define the clinicopathological status (PyVCPS). Incidence of acute rejection was 28% in the 2(nd) biopsy and 50% subsequently (25% mixed TCMR+AMR; rejection overall affected 38% of patients (>50% AMR)...
April 19, 2017: American Journal of Transplantation
https://www.readbyqxmd.com/read/28422378/cellular-retinoic-acid-bioavailability-in-various-pathologies-and-its-therapeutic-implication
#4
REVIEW
Makoto Osanai
Retinoic acid (RA), an active metabolite of vitamin A, is a critical signaling molecule in various cell types. We found that RA depletion caused by expression of the RA-metabolizing enzyme CYP26A1 promotes carcinogenesis, implicating CYP26A1 as a candidate oncogene. Several studies of CYP26s have suggested that the biological effect of RA on target cells is primarily determined by "cellular RA bioavailability", which is defined as the RA level in an individual cell, rather than by the serum concentration of RA...
April 19, 2017: Pathology International
https://www.readbyqxmd.com/read/28417047/clinical-significance-of-snp-rs2596542-in-histocompatibility-complex-class-i-related-gene-a-promoter-region-among-hepatitis-c-virus-related-hepatocellular-carcinoma-cases
#5
Amal A Mohamed, Ola M Elsaid, Eman A Amer, Heba H Elosaily, Mohamed I Sleem, Shawkat S Gerges, Mohamed A Saleh, Amal El Shimy, Yasmine S El Abd
The major histocompatibility complex class I-related gene A (MICA) is an antigen induced by stress and performs an integral role in immune responses as an anti-infectious and antitumor agent. This work was designed to investigate whether (SNP) rs2596542C/T in MICA promoter region is predictive of liver cirrhosis (LC) and hepatocellular carcinoma (HCC) or not. Forty-seven healthy controls and 94 HCV-infected patients, subdivided into 47 LC and 47 HCC subjects were enrolled in this study. SNP association was studied using real time PCR and soluble serum MICA concentration was measured using ELISA...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28412995/development-and-validation-of-prediction-models-for-risks-of-complications-in-early-onset-pre-eclampsia-prep-a-prospective-cohort-study
#6
Shakila Thangaratinam, John Allotey, Nadine Marlin, Ben W Mol, Peter Von Dadelszen, Wessel Ganzevoort, Joost Akkermans, Asif Ahmed, Jane Daniels, Jon Deeks, Khaled Ismail, Ann Marie Barnard, Julie Dodds, Sally Kerry, Carl Moons, Richard D Riley, Khalid S Khan
BACKGROUND: The prognosis of early-onset pre-eclampsia (before 34 weeks' gestation) is variable. Accurate prediction of complications is required to plan appropriate management in high-risk women. OBJECTIVE: To develop and validate prediction models for outcomes in early-onset pre-eclampsia. DESIGN: Prospective cohort for model development, with validation in two external data sets. SETTING: Model development: 53 obstetric units in the UK...
April 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28412975/effect-of-cryopreservation-on-proliferation-and-differentiation-of-periodontal-ligament-stem-cell-sheets
#7
Mengying Li, Cheng Feng, Xiuge Gu, Qin He, Fulan Wei
BACKGROUND: Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets...
April 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412296/impact-of-higher-order-heme-degradation-products-on-hepatic-function-and-hemodynamics
#8
Raphael A Seidel, Thierry Claudel, Franziska A Schleser, Navin K Ojha, Matthias Westerhausen, Sandor Nietzsche, Christoph Sponholz, Frans Cuperus, Sina M Coldewey, Stefan H Heinemann, Georg Pohnert, Michael Trauner, Michael Bauer
BACKGROUND& AIMS: While, traditionally, biliverdin and bilirubin are considered end products of heme catabolism, there is evidence for further degradation to diverse bioactive products. As such, Z-BOX A and Z-BOX B arise upon oxidation with unknown implications for hepatocellular function and integrity. We studied the impact of Z-BOX A and B on hepatic functions and explored their alterations in health and cholestatic conditions. METHODS: Functional implications and mechanisms were investigated in rats, hepatocytic HepG2 and HepaRG cells, human immortalized hepatocytes, and isolated perfused livers...
April 12, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28410798/protective-role-of-coq10-or-l-carnitine-on-the-integrity-of-the-myocardium-in-doxorubicin-induced-toxicity
#9
Hesham N Mustafa, Gehan A Hegazy, Sally A El Awdan, Marawan AbdelBaset
Doxorubicin (DOX) is a chemotherapeutic agent used for treatment of different cancers and its clinical usage is hindered by the oxidative injury-related cardiotoxicity. This work aims to declare if the harmful effects of DOX on heart can be alleviated with the use of Coenzyme Q10 (CoQ10) or L-carnitine. The study was performed on seventy two female Wistar albino rats divided into six groups, 12 animals each: Control group; DOX group (10mg/kg); CoQ10 group (200mg/kg); L-carnitine group (100mg/kg); DOX+CoQ10 group; DOX+L-carnitine group...
April 2, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28409620/surface-atomic-structure-directs-the-fate-of-human-mesenchymal-stem-cells
#10
Lingqing Dong, Kui Cheng, Ying Zhou, Mengfei Yu, Jiaxing Gong, Yihan Lin, Qi Luo, Qi Wang, Wen-Jian Weng, Huiming Wang
Stem cells in contact with materials are able to sense their surface features, integrate extracellular matrix (ECM) protein cues via signal transduction pathway and ultimately direct cell fate decisions. However, discovering the interdisciplinary mechanisms of how stem cells response to inherent material surface features still remains a challenge due to the complex, multicomponent signalling milieu present in ECM environment. Here, we demonstrate that the fate of human mesenchymal stem cells (hMSCs) can be regulated by inherent physical cue of material surface down to atomic-scale features...
April 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28409016/surface-complement-c3-fragments-and-cellular-binding-of-microparticles-in-patients-with-sle
#11
Line Kjær Winberg, Claus Henrik Nielsen, Søren Jacobsen
OBJECTIVES: To examine microparticles (MPs) from patients with SLE and healthy controls (HCs) by determining the cellular origin of the MPs, quantifying attached fragments of complement component 3 (C3) and assessing the ability of MPs to bind to circulating phagocytes and erythrocytes. These features may be relevant for clearance of MPs in SLE pathogenesis. METHODS: Attached C3 fragments (C3b, iC3b, C3d), membrane integrity and cell surface markers of MPs from 18 patients with SLE and 11 HCs were measured by adding specific antibodies, 7-aminoactinomycin D (7AAD) and annexin V...
2017: Lupus Science & Medicine
https://www.readbyqxmd.com/read/28406230/meta-signature-lncrnas-serve-as-novel-biomarkers-for-colorectal-cancer-integrated-bioinformatics-analysis-experimental-validation-and-diagnostic-evaluation
#12
Meiyu Dai, Xiaoli Chen, Shanying Mo, Jinwan Li, Zhizhuo Huang, Shifeng Huang, Junyi Xu, Baoyu He, Yan Zou, Jingfan Chen, Shengming Dai
The aim of this study is to explore the differentially expressed lncRNAs, which may have potential biological function and diagnostic value in colorectal cancer (CRC). Through integrated data mining, we finally identified nine differentially expressed lncRNAs and their potential mRNA targets. After a series of bioinformatics analyses, we screened significant pathways and GO terms that are related to the up-regulated and down-regulated transcripts respectively. Meanwhile, the nine lncRNAs were validated in 30 paired tissues and cell lines by qRT-PCR and the results were basically consistent with the microarray data...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28404531/an-integrated-method-of-therapeutic-lifestyle-change-for-older-adults-with-dyslipidaemia
#13
J-Y Zhao, H Li, S Jin, X-H Chen, L-L Chen
OBJECTIVES: To evaluate the effect of therapeutic lifestyle change with a non-pharmacological intervention method in older adults with dyslipidaemia. STUDY DESIGN: Stratified randomized trial. METHODS: Participants with dyslipidaemia (n = 214) aged ≥60 years were randomized to the conventional guide group, the educational course (EC) group, the telephone call (PC) group or the PC + EC group for 24 weeks. Total cholesterol, triglyceride, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and the knowledge, attitude and practice score for serum lipids were measured at baseline (T1), mid-intervention (week 12, T2) and post-intervention (week 24, T3)...
April 5, 2017: Public Health
https://www.readbyqxmd.com/read/28402551/a-risk-prediction-score-for-acute-kidney-injury-in-the-intensive-care-unit
#14
Rakesh Malhotra, Kianoush B Kashani, Etienne Macedo, Jihoon Kim, Josee Bouchard, Susan Wynn, Guangxi Li, Lucila Ohno-Machado, Ravindra Mehta
Background.: Acute kidney injury (AKI) is common in critically ill patients and is associated with high morbidity and mortality. Early identification of high-risk patients provides an opportunity to develop strategies for prevention, early diagnosis and treatment of AKI. Methods.: We undertook this multicenter prospective cohort study to develop and validate a risk score for predicting AKI in patients admitted to an intensive care unit (ICU). Patients were screened for predictor variables within 48 h of ICU admission...
April 10, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28400812/edaravone-protects-against-oxygen-glucose-serum-deprivation-restoration-induced-apoptosis-in-spinal-cord-astrocytes-by-inhibiting-integrated-stress-response
#15
Bin Dai, Ting Yan, Yi-Xing Shen, You-Jia Xu, Hai-Bin Shen, Dong Chen, Jin-Rong Wang, Shuang-Hua He, Qi-Rong Dong, Ai-Liang Zhang
We previously found that oxygen-glucose-serum deprivation/restoration (OGSD/R) induces apoptosis of spinal cord astrocytes, possibly via caspase-12 and the integrated stress response, which involves protein kinase R-like endoplasmic reticulum kinase (PERK), eukaryotic initiation factor 2-alpha (eIF2α) and activating transcription factor 4 (ATF4). We hypothesized that edaravone, a low molecular weight, lipophilic free radical scavenger, would reduce OGSD/R-induced apoptosis of spinal cord astrocytes. To test this, we established primary cultures of rat astrocytes, and exposed them to 8 hours/6 hours of OGSD/R with or without edaravone (0...
February 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28400465/hypercholesterolemia-impairs-oxytocin-induced-uterine-contractility-in-late-pregnant-mouse
#16
Amol R Padol, Susanth V Sukumaran, Abdul Sadam, Manickam Kesavan, Kandasamy Arunvikram, Ankita D Verma, Vivek Srivastava, Manjit Panigrahi, Thakur Uttam Singh, Avinash G Telang, Santosh K Mishra, Subhashree Parida
High cholesterol is known to negatively affect uterine contractility in ex vivo conditions. The aim of the present study was to reveal the effect of in vivo hypercholesterolemia on spontaneous and oxytocin-induced uterine contractility in late pregnant mouse uterus. Female Swiss albino mice were fed with high cholesterol (HC) diet (0.5% sodium cholate, 1.25% cholesterol and 15% fat) for 6 weeks and then throughout the gestation period after mating. On day 19 of gestation, serum cholesterol level was increased more than 3-fold while triglycerides level was reduced in HC diet-fed animals as compared to control animals fed with a standard diet...
May 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28398368/-the-apulia-hf-multicenter-study-efficacy-of-a-management-protocol-shared-between-hospital-and-territorial-health-services-for-acute-decompensated-heart-failure-patients
#17
Massimo Iacoviello, Sergio Pede, Nadia Aspromonte, Francesca Bux, Mariligia Panunzio, Vittorio Donadeo, Alessandra De Castro, Ettore Antoncecchi, Nicola D'Amato, Elisabetta Squiccimarro, Bartolomeo Silvestri, Lucia Malerba, Luisa De Gennaro, Giuseppe Modugno, Elena Serafini, Biagio Curci, Dario Grande, Furio Colivicchi, Giovanni De Luca, Gianfranco Ignone, Carlo D'Agostino, Pasquale Caldarola
BACKGROUND: The aim of this study was to demonstrate the efficacy of a management model to reduce hospitalizations in patients recently admitted for acute decompensated heart failure. METHODS: The management model was based on a close integration between hospital and territory health services. Clinical evaluation, ECG, echocardiographic findings, total body bioimpedance and brain natriuretic peptide serum levels were used to assess clinical stability of patients at discharge and during follow-up...
February 2017: Giornale Italiano di Cardiologia
https://www.readbyqxmd.com/read/28396861/non-invasive-investigations-for-the-diagnosis-of-fontan-associated-liver-disease-in-pediatric-and-adult-fontan-patients
#18
Amyna Fidai, Frederic Dallaire, Nanette Alvarez, Yvonne Balon, Robin Clegg, Michael Connelly, Frank Dicke, Deborah Fruitman, Joyce Harder, Kimberley Myers, David J Patton, Tim Prieur, Erika Vorhies, Robert P Myers, Steven R Martin, Steven C Greenway
Fontan-associated liver disease (FALD) is a serious complication related to the chronically elevated venous pressure and low cardiac output of this abnormal circulation. However, diagnostic markers for this condition are limited. We hypothesized that specific tests for fibrosis developed for other chronic liver diseases would identify a higher prevalence of FALD than ultrasound and standard laboratory tests and that identified abnormalities would correlate with time post-Fontan. In this cross-sectional study, we assessed 19 children (average age 8...
2017: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28396511/a-database-augmented-exosome-based-mass-spectrometry-approach-exemplarily-identifies-circulating-claudin-3-as-biomarker-in-prostate-cancer
#19
Thomas Stefan Worst, Jost von Hardenberg, Julia Christina Gross, Philipp Erben, Martina Schnoelzer, Ingrid Hausser, Peter Bugert, Maurice Stephan Michel, Michael Boutros
In prostate cancer and other malignancies sensitive and robust biomarkers are lacking or have relevant limitations. Prostate specific antigen (PSA), the only biomarker widely used in prostate cancer, is suffering from low specificity. Exosomes offer new perspectives in the discovery of blood-based biomarkers. Here we present a proof-of principle study for a proteomics-based identification pipeline, implementing existing data sources, to exemplarily identify exosome-based biomarker candidates in prostate cancer...
April 9, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28396253/moderate-therapeutic-hypothermia-induces-multimodal-protective-effects-in-oxygen-glucose-deprivation-reperfusion-injured-cardiomyocytes
#20
Jana Krech, Giang Tong, Sylvia Wowro, Christoph Walker, Lisa-Maria Rosenthal, Felix Berger, Katharina Rose Luise Schmitt
OBJECTIVE: Therapeutic hypothermia has been shown to attenuate myocardial cell death due to ischemia/reperfusion injury. However, cellular mechanisms of cooling remain to be elucidated. Especially during reperfusion, mitochondrial dysfunction contributes to cell death by releasing apoptosis inductors. The aim of the present study was to investigate the effects of moderate therapeutic hypothermia (33.5°C) on mitochondrial mediated apoptosis in ischemia/reperfusion-injured cardiomyocytes...
April 7, 2017: Mitochondrion
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