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https://www.readbyqxmd.com/read/28718841/development-of-an-immunosensor-for-pfhrp-2-as-a-biomarker-for-malaria-detection
#1
Aver Hemben, Jon Ashley, Ibtisam E Tothill
Plasmodium falciparum histidine-rich protein 2 (PfHRP 2) was selected in this work as the biomarker for the detection and diagnosis of malaria. An enzyme-linked immunosorbent assay (ELISA) was first developed to evaluate the immunoreagent's suitability for the sensor's development. A gold-based sensor with an integrated counter and an Ag/AgCl reference electrode was first selected and characterised and then used to develop the immunosensor for PfHRP 2, which enables a low cost, easy to use, and sensitive biosensor for malaria diagnosis...
July 18, 2017: Biosensors
https://www.readbyqxmd.com/read/28715013/self-assembling-covalent-organic-framework-functionalized-magnetic-graphene-hydrophilic-biocomposites-as-an-ultrasensitive-matrix-for-n-linked-glycopeptide-recognition
#2
Jiaxi Wang, Jie Li, Mingxia Gao, Xiangmin Zhang
The development of additional functions and applications of covalent organic framework (COF)-derived materials still remains highly desired. In our work, a novel COF-functionalized magnetic graphene biocomposite (MagG@COF-5) was first developed as an ultrasensitive hydrophilic matrix via a facile self-assembly method for efficiently recognizing N-linked glycopeptides. By integrating the characteristics of the magnetic graphene and COF-5 layer, the MagG@COF-5 owns features of an outstanding magnetic response, a high specific area, strong hydrophilic properties and a unique size-exclusion effect...
July 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28714127/high-hepatitis-e-virus-antibody-positive-rates-in-dogs-and-humans-exposed-to-dogs-in-the-south-west-of-china
#3
M Y Zeng, H Gao, X X Yan, W J Qu, Y K Sun, G W Fu, Y L Yan
Hepatitis E (HE) is a zoonotic viral disease caused by hepatitis E virus (HEV). The objective of this study was to investigate the prevalence of HEV infection among dogs and humans exposed to dogs in the south-west region of China. A total of 4,490 dog serum samples and 2,206 relative practitioner serum samples were collected from 18 pet hospitals and dog farms in Yunnan, Sichuan and Guizhou province, and the anti-HEV IgG antibodies were detected by ELISA. The results showed that the total positive rate of anti-HEV antibodies was 36...
July 16, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28709869/protective-effect-of-bacillopeptidase-cfr5-from-bacillus-subtilis-cfr5-on-cerulein-induced-pancreatitis
#4
G R Sharmila, G Venkateswaran
Bacillopeptidase is a serine peptidase, known for its fibrinolytic activity. However, a very little information is known about its in vivo inflammatory and/or anti-inflammatory properties. Thus, to understand whether bacillopeptidase incorporation can regulate pancreatitis or not, the cerulein-induced pancreatitis model was used, and the role of bacillopeptidase on pancreatitis was studied. In this study, 46 kDa protein was purified from Bacillus subtilis and identified as bacillopeptidase CFR5 (BPC) through MS/MS analysis...
July 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28709158/dexmedetomidine-protects-against-multi-organ-dysfunction-induced-by-heatstroke-via-sustaining-the-intestinal-integrity
#5
Zhen-Na Xia, Ying Zong, Zi-Teng Zhang, Ji-Kuai Chen, Xiu-Juan Ma, Yan-Gang Liu, Li-Jun Zhao, Guo-Cai Lu
Previous studies have indicated that gut-derived endotoxin played a pivotal role for aggravating systemic inflammatory response to multi-organ dysfunction under heatstroke. Dexmedetomidine (DEX) could protect against inflammation and multi-organ injury in various scenarios. The aim of this study was to explore the protective effect of DEX on heatstroke and the mechanism involved. Male C57BL/6 mice were placed in a controlled climate chamber (40 ± 1°C) until the maximum core temperature (Tc, Max) of 42...
August 2017: Shock
https://www.readbyqxmd.com/read/28709088/integrated-amplified-aptasensor-with-in-situ-precise-preparation-of-copper-nanoclusters-for-ultrasensitive-electrochemical-detection-of-microrna-21
#6
Yijia Wang, Xinying Zhang, Lang Zhao, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang
MicroRNA 21 (MIR21) has garnered much attention in recent years as an important disease biomarker. The detection of it in human system shows great significance for the healthy evaluation and major diseases early detection. Herein, a novel approach tactfully manipulates the in-situ precise preparation of copper nanoclusters on overlapping Y-shaped ds-DNA for MIR21 analysis were developed in the proposed integrated aptasensor. In the presence of target MIR21, overlapping Y-shaped ds-DNA was constructed on electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28708392/thiol-triggered-release-of-intraliposomal-content-from-liposomes-made-of-extremophile-inspired-tetraether-lipids
#7
Takaoki Koyanagi, Jessica L Cifelli, Geoffray Leriche, David Onofrei, Gregory P Holland, Jerry Yang
Liposomal drug delivery systems have been used for delivery of drugs to targeted tissues while reducing unwanted side effects. DOXIL®, for instance, is a liposomal formulation of the anti-cancer agent doxorubicin (DOX) that has been used to address problems associated with non-specific toxicity of free DOX. However, while this liposomal formulation allows for a more stable circulation of doxorubicin in the body compared with free drug, efficacy for cancer therapy is reduced when compared with systemic injections of free drug...
July 14, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28704805/biothiols-induced-colour-change-of-silver-nanoparticles-a-colorimetric-sensing-strategy
#8
Ambily Thomas, Unni Sivasankaran, Krishnapillai Girish Kumar
A sensor for the detection and determination of bio-thiols (glutathione (GSH) and cysteamine (Cyste)) has been developed by integrating the distinguished distance related optical characteristics of silver nanoparticles with the simplicity of colorimetric technique. In presence of these analytes, shift in surface plasmon resonance (SPR) absorption of silver nanoparticles (AgNPs) with change in its colour was observed. Yellow coloured AgNPs solution becomes colourless in presence of GSH and changes to red in presence of Cyste...
July 2, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28701994/the-role-of-substance-p-in-secondary-pathophysiology-after-traumatic-brain-injury
#9
REVIEW
Robert Vink, Levon Gabrielian, Emma Thornton
It has recently been shown that substance P (SP) plays a major role in the secondary injury process following traumatic brain injury (TBI), particularly with respect to neuroinflammation, increased blood-brain barrier (BBB) permeability, and edema formation. Edema formation is associated with the development of increased intracranial pressure (ICP) that has been widely associated with increased mortality and morbidity after neurotrauma. However, a pharmacological intervention to specifically reduce ICP is yet to be developed, with current interventions limited to osmotic therapy rather than addressing the cause of increased ICP...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28701387/high-iron-stores-in-the-low-malaria-season-increase-malaria-risk-in-the-high-transmission-season-in-a-prospective-cohort-of-rural-zambian-children
#10
Maxwell A Barffour, Kerry J Schulze, Christian L Coles, Justin Chileshe, Ng'andwe Kalungwana, Margia Arguello, Ward Siamusantu, William J Moss, Keith P West, Amanda C Palmer
Background: Higher iron stores, defined by serum ferritin (SF) concentration, may increase malaria risk.Objective: We evaluated the association between SF assessed during low malaria season and the risk of malaria during high malaria season, controlling for inflammation.Methods: Data for this prospective study were collected from children aged 4-8 y (n = 745) participating in a biofortified maize efficacy trial in rural Zambia. All malaria cases were treated at baseline (September 2012). We used baseline SF and malaria status indicated by positive microscopy at endline (March 2013) to define exposure and outcome, respectively...
July 12, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28700829/fast-sensitive-and-quantitative-point-of-care-platform-for-the-assessment-of-drugs-of-abuse-in-urine-serum-and-whole-blood
#11
Ying Li, Uvaraj Uddayasankar, Bangshun He, Ping Wang, Lidong Qin
Drug abuse is a major public health problem in many countries in Europe and North America. Currently available platforms for drug abuse assessment are facing technical challenges of non-quantitation, inaccuracy, low throughput, incompatible with diverse complex specimens, long assay time and requirement of instrument and/or expertise for readout. Here, we report an integrated Competitive Volumetric-bar-chart Chip (CV-Chip) to assay multiple drug targets at the point-of-care (POC). To the best of our knowledge, it is the first time that a POC platform has been demonstrated to fully address the above-mentioned limitations...
July 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28700482/beneficial-effects-of-fu-zheng-qu-zhuo-oral-liquid-combined-with-standard-integrated-therapy-in-patients-with-chronic-kidney-disease-stage-3-4-a-randomized-placebo-controlled-clinical-trial
#12
Shen Li, Xiang-Rong Rao, Xi-Wen Dai, Kun Pei, Lee Wang, Bao-Min Huo, Xiu-Juan Wang, Ling-Xin Kong, Nan-Nan Zhang, Feng-Mei Lian
BACKGROUND: The high worldwide prevalence of chronic kidney disease (CKD) is a critical health problem and the development of more effective therapies is urgently needed. We conducted a randomized, double-blinded, placebo-controlled clinical trial from October 2010 to December 2012 to assess whether Fu-Zheng-Qu-Zhuo oral liquid (FZQZ) has a beneficial effect in preventing CKD progression when added to standard integrated therapies. METHODS: Patients with CKD stage 3 to 4 from 3 hospitals in Beijing, China were enrolled...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28697126/effect-of-serum-triglycerides-on-clinical-outcomes-in-acute-pancreatitis-findings-from-a-regional-integrated-health-care-system
#13
Laura Y Sue, Michael Batech, Dhiraj Yadav, Stephen J Pandol, William A Blumentals, Lenore Soodak von Krusenstiern, Wansu Chen, Bechien U Wu
OBJECTIVES: The aim of this study was to evaluate the effect of serum triglycerides on the development of multiple or persistent organ failure in patients with acute pancreatitis. METHODS: A retrospective cohort study was conducted among patients hospitalized for acute pancreatitis between 2006 and 2013. Triglyceride levels measured before and within 72 hours of admission were compared. In addition, the effect of triglyceride levels on the development of multiple or persistent organ failure during hospitalization for acute pancreatitis was assessed...
August 2017: Pancreas
https://www.readbyqxmd.com/read/28690088/a-cluster-randomized-trial-to-estimate-the-effect-of-mobile-screening-and-treatment-feedback-on-hba1c-and-diabetes-related-complications-in-tshwane-primary-health-care-clinics-south-africa
#14
Elizabeth Melanie Webb, Paul Rheeder
AIM AND OBJECTIVES: Our study had two primary objectives. The first was to evaluate the effect of an expert review with an individualised patient management plan on change in HbA1c. The second was to determine whether a mobile screening unit would increase the proportion of patients screened for glycaemic and lipid control, as well as diabetic complications as compared to the standard guideline-driven clinic care. METHODS: We conducted a cluster-randomised trial and enrolled 599 diabetic patients from twelve primary health care clinics in the Tshwane district of South Africa...
July 6, 2017: Primary Care Diabetes
https://www.readbyqxmd.com/read/28689350/repeated-exposure-to-cat-urine-induces-complex-behavioral-hormonal-and-c-fos-mrna-responses-in-norway-rats-rattus-norvegicus
#15
Baofa Yin, Chen Gu, Yi Lu, Ibrahim M Hegab, Shengmei Yang, Aiqin Wang, Wanhong Wei
Prey species show specific adaptations that allow recognition, avoidance, and defense against predators. This study was undertaken to investigate the processing of a chronic, life-threatening stimulus to Norway rats (Rattus norvegicus). One hundred forty-four Norway rats were tested by repeated presentation of cat urine for 1 h at different days in a defensive withdrawal apparatus. Rats exposed to urine for short periods showed significantly larger defensive behavioral and medial hypothalamic c-fos messenger RNA (mRNA) responses than other groups...
August 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28688970/secretory-stanniocalcin-1-promotes-metastasis-of-hepatocellular-carcinoma-through-activation-of-jnk-signaling-pathway
#16
Kristy Kwan-Shuen Chan, Carmen Oi-Ning Leung, Carmen Chak-Lui Wong, Daniel Wai-Hung Ho, Kenneth Siu-Ho Chok, Ching-Lung Lai, Irene Oi-Lin Ng, Regina Cheuk-Lam Lo
The hypoxic microenvironment is well-characterized in hepatocellular carcinoma (HCC). Delineation of hypoxia-responsive events is an integral part to understand the pathogenesis of HCC. We studied the functional role and clinical relevance of Stanniocalcin 1 (STC1), a hypoxia-induced molecular target, in HCC. In our clinical cohort, STC1 transcript was up-regulated in HCC tumor tissues. Moreover, STC1 protein was detected in the sera of HCC patients. A higher serum STC1 level was correlated with larger tumor size and poorer 5-year disease-free survival...
July 6, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28687362/the-unexpected-function-of-a-highly-conserved-yxx%C3%AE-motif-in-hcv-core-protein
#17
Eirini Karamichali, Elisavet Serti, Aikaterini Gianneli, Aikaterini Papaefthymiou, Athanassios Kakkanas, Pelagia Foka, Alexandros Seremetakis, Konstantina Katsarou, Ioannis P Trougakos, Urania Georgopoulou
Hepatitis C virus (HCV) is an RNA positive strand virus, member of the Flaviviridae family. The HCV viral particle is composed of a capsid containing the genome, surrounded by an endoplasmic reticulum (ER)-derived lipid bilayer where E1 and E2 are assembled as heterodimers. However, different forms of viral particles have been identified in the serum of HCV-infected patients, including non-enveloped particles. Previous reports have demonstrated that HCV non-enveloped capsid-like particles (HCVne) can be generated by HCV core protein sequence...
July 4, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28684378/utility-of-bone-marrow-biopsy-and-aspirate-for-staging-of-diffuse-large-b-cell-lymphoma-in-the-era-of-positron-emission-tomography-with-2-deoxy-2-fluorine-18-fluoro-deoxyglucose-integrated-with-computed-tomography
#18
Prakash Vishnu, Andrew Wingerson, Marie Lee, Margaret T Mandelson, David M Aboulafia
BACKGROUND: About one-third of patients with diffuse large B cell lymphoma (DLBCL) have lymphomatous bone marrow involvement (BMI) at the time of diagnosis, and bone marrow aspirate/biopsy (BMAB) is considered the gold standard to detect such involvement. [(18)F] fluorodeoxyglucose positron emission tomography combined with computed tomography (PET-CT), has become standard pretreatment imaging in DLBCL and may be a noninvasive alternative to BMAB to ascertain BMI. Prior studies have suggested that PET-CT scan may obviate the need for BMAB as a component for staging patients with newly diagnosed DLBCL, but this is not yet a standard of practice...
June 17, 2017: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/28684113/sericin-improves-heart-and-liver-mitochondrial-architecture-in-hypercholesterolaemic-rats-and-maintains-pancreatic-and-adrenal-cell-biosynthesis
#19
Sumate Ampawong, Duangnate Isarangkul, Pornanong Aramwit
Hypercholesterolaemia is well known to be associated with mitochondrial dysfunction, subsequently leading to multiple organ failure. Similar to other natural products, sericin is a candidate for adjunctive therapy in hyperlipidaemic conditions. However, the cholesterol-lowering mechanisms of sericin are multifactorial and controversial. Here, a high-cholesterol-fed rat model with or without sericin treatment was established using a dosage of 1,000mg/kg/day for 30 days. Blood lipid profiles, oxidative stress markers (superoxide dismutase, SOD; malondialdehyde, MDA; nuclear factor erythroid 2-related factor, Nrf-2), dysmorphic mitochondria in relation to fission (dynamin-related protein-1; Drp-1) and fusion (guanosine triphosphatase mutated in dominant optic atrophy; OPA-1) markers and biosynthetic markers (aquaporin, AQP-1; tubulin-4β, Tb4B) in the pancreas and adrenal gland were evaluated...
July 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28681814/resurgence-of-diphtheria-in-rural-areas-of-north-karnataka-india
#20
Mahantesh V Parande, Subarna Roy, B G Mantur, Aisha M Parande, Rupali S Shinde
BACKGROUND: A diphtheria outbreak was identified from Vijayapura (formerly Bijapur) district in the South Indian state of Karnataka in 2011. There was a surge in the number of throat swab samples received under the Integrated Disease Surveillance Programme (IDSP) in North Karnataka since then. OBJECTIVES: A microbiological study was undertaken to generate information on the status of resurgence of the disease in the region. MATERIALS AND METHODS: Throat swabs from 432 suspected cases of diphtheria during 2012-2015 were obtained from government hospitals and primary health centres of 8 districts in North Karnataka and were processed for the culture and identification of Corynebacterium diphtheriae...
April 2017: Indian Journal of Medical Microbiology
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