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https://www.readbyqxmd.com/read/27911598/type-3-deiodinase-role-on-central-thyroid-hormone-action-affects-the-leptin-melanocortin-system-and-circadian-activity
#1
Zhaofei Wu, M Elena Martinez, Donald L St Germain, Arturo Hernandez
The role of thyroid hormones (TH) in the central regulation of energy balance is increasingly appreciated. Mice lacking the type 3 deiodinase (DIO3), which inactivates TH, have decreased circulating TH levels relative to control mice due to defects in the hypothalamic-pituitary-thyroid axis. However, we have shown that the TH status of the adult Dio3(-/-) brain is opposite that of the serum, exhibiting enhanced levels of TH action. Since the brain, specially the hypothalamus, harbors important circuitries that regulate metabolism, we aimed to examine the energy balance phenotype of Dio3(-/-) mice and determine whether it is associated with hypothalamic abnormalities...
December 2, 2016: Endocrinology
https://www.readbyqxmd.com/read/27910788/metabolomic-investigation-of-rat-serum-following-oral-administration-of-the-willow-bracket-medicinal-mushroom-phellinus-igniarius-agaricomycetes-by-uplc-hdms
#2
Yu Dong, Ying He, Zhongming Yu, Yang Zhang, Nani Wang, Dan Shou, Changyu Li
The medicinal willow bracket mushroom, Phellinus igniarius, is a species that has been reported to possess antibacterial, antioxidative, antitumor, antidiabetic, and antihyperlipidemia activities. The aim of this study was to elucidate the changes in endogenous metabolites after oral administration of a decoction of Ph. Igniarius. Ultraperformance liquid chromatography (UPLC)/electrospray ionization synapt high-definition mass spectrometry (ESI-HDMS) combined with pattern recognition approaches, including principal component analysis and orthogonal partial least squares discriminant analysis, were integrated to discover differentiating metabolites...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910726/the-effect-of-deuterium-oxide-on-the-conformational-stability-and-aggregation-of-bovine-serum-albumin
#3
Mouhamad Reslan, Veysel Kayser
Protein aggregation is a significant problem affecting the integrity of proteins, and is a major hindrance to the development of biopharmaceutical products. Deuterium Oxide (D2O), widely used in protein characterization studies, has been shown to promote protein aggregation when used as a substitute for water in most buffered protein solutions; however, a few studies have reported minor improvements in melting point temperatures for some proteins. Our study aims to investigate the effect of D2O on protein stability, using bovine serum albumin (BSA) as a model...
December 2, 2016: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/27908708/chlorogenic-acid-ameliorates-isoproterenol-induced-myocardial-injury-in-rats-by-stabilizing-mitochondrial-and-lysosomal-enzymes
#4
Palaniyandi Akila, Lourthurani Asaikumar, Lakshmanan Vennila
This study was deliberated to aspire the effects of chlorogenic acid (CGA) against myocardial infarction (MI) induced by Isoproterenol (ISO), in a rat model. In the pathology of MI, enzymes released due to the mitochondrial and lysosomal lipid peroxidation play an integral role. Induction of rats with ISO (85mg/kg BW) for 2 consecutive days resulted in a significant decrease in the activities of heart mitochondrial enzymes isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH)...
November 28, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27907005/intraluminal-flagellin-differentially-contributes-to-gut-dysbiosis-and-systemic-inflammation-following-burn-injury
#5
Logan Grimes, Allie Doyle, Aaron L Miller, Richard B Pyles, Gabor Olah, Csaba Szabo, Sarah Hoskins, Tonyia Eaves-Pyles
Burn injury is associated with a loss of gut barrier function, resulting in systemic dissemination of gut-derived bacteria and their products. The bacterial protein and TLR5 agonist, flagellin, induces non-specific innate immune responses. Because we detected flagellin in the serum of burn patients, we investigated whether gut-derived flagellin was a primary or secondary contributor to intestinal dysfunction and systemic inflammation following burn injury. The apical surface of polarized human intestinal epithelial cells (IECs), Caco-2BBe, were exposed to 50 or 500 ng of purified flagellin and 1 x 105 of an intestinal E...
2016: PloS One
https://www.readbyqxmd.com/read/27903845/protein-biomarkers-of-mood-disorders
#6
EDITORIAL
Carmen Rodríguez Cerdeira, Elena Sánchez-Blanco, Beatriz Sánchez-Blanco, Jose Luis González-Cespón
Psychiatric evaluation presents a significant challenge because it conceptually integrates the input from multiple psychopathological approaches. Recent technological advances in the study of protein structure, function, and interactions have provided a breakthrough in the diagnosis and treatment of mood disorders (MD), and have identified novel biomarkers to be used as indicators of normal and disease states or response to drug treatment. The investigation of biomarkers for psychiatric disorders, such as enzymes (catechol-O-methyl transferase and monoamine oxidases) or neurotransmitters (dopamine, serotonin, norepinephrine) and their receptors, particularly their involvement in neuroendocrine activity, brain structure, and function, and response to psychotropic drugs, should facilitate the diagnosis of MD...
November 30, 2016: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/27903281/improving-the-clinical-management-of-traumatic-brain-injury-through-the-pharmacokinetic-modeling-of-peripheral-blood-biomarkers
#7
Aaron Dadas, Jolewis Washington, Nicola Marchi, Damir Janigro
BACKGROUND: Blood biomarkers of neurovascular damage are used clinically to diagnose the presence severity or absence of neurological diseases, but data interpretation is confounded by a limited understanding of their dependence on variables other than the disease condition itself. These include half-life in blood, molecular weight, and marker-specific biophysical properties, as well as the effects of glomerular filtration, age, gender, and ethnicity. To study these factors, and to provide a method for markers' analyses, we developed a kinetic model that allows the integrated interpretation of these properties...
November 30, 2016: Fluids and Barriers of the CNS
https://www.readbyqxmd.com/read/27899683/validation-of-an-integrative-mathematical-model-of-dehydration-and-rehydration-in-virtual-humans
#8
W Andrew Pruett, John S Clemmer, Robert L Hester
Water homeostasis is one of the body's most critical tasks. Physical challenges to the body, including exercise and surgery, almost always coordinate with some change in water handling reflecting the changing needs of the body. Vasopressin is the most important hormone that contributes to short-term water homeostasis. By manipulating vascular tone and regulating water reabsorption in the collecting duct of the kidneys, vasopressin can mediate the retention or loss of fluids quickly. In this study, we validated HumMod, an integrative mathematical model of human physiology, against six different challenges to water homeostasis with special attention to the secretion of vasopressin and maintenance of electrolyte balance...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27895429/potential-model-for-differential-diagnosis-between-crohn-s-disease-and-primary-intestinal-lymphoma
#9
Tian-Yu Zhang, Yun Lin, Rong Fan, Shu-Rong Hu, Meng-Meng Cheng, Mao-Chen Zhang, Li-Wen Hong, Xiao-Lin Zhou, Zheng-Ting Wang, Jie Zhong
AIM: To evaluate the usefulness of different parameters to differentiate Crohn's disease (CD) from primary intestinal lymphoma (PIL). METHODS: The medical records of 85 patients with CD and 56 patients with PIL were reviewed retrospectively. Demographic, clinical, laboratory, endoscopic, and computed tomographic enterography (CTE) parameters were collected. The univariate value of each parameter was analyzed. A differentiation model was established by pooling all the valuable parameters...
November 14, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27888023/integrated-process-for-the-purification-and-immobilization-of-the-envelope-protein-domain-iii-of-dengue-virus-type-2-expressed-in-rachiplusia-nu-larvae-and-its-potential-application-in-a-diagnostic-assay
#10
María Emilia Smith, Alexandra Marisa Targovnik, Julieta Cerezo, María Alejandra Morales, María Victoria Miranda, Julián Rodríguez Talou
Dengue incidence has grown dramatically in the last years, with about 40% of the world population at risk of infection. Recently, a vaccine developed by Sanofi Pasteur has been registered, but only in a few countries. Moreover, specific antiviral drugs are not available. Thus, an efficient and accurate diagnosis is important for disease management. To develop a low-cost immunoassay for dengue diagnosis, in the present study we expressed the envelope protein domain III of dengue virus type 2 in Rachiplusia nu larvae by infection with a recombinant baculovirus...
November 22, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27885054/impact-of-uteroplacental-insufficiency-on-postnatal-rat-male-gonad
#11
Valentina Pampanini, Daniela Germani, Antonella Puglianiello, Jan-Bernd Stukenborg, Ahmed Reda, Iuliia Savchuk, Kristin Ros Kjartansdóttir, Stefano Cianfarani, Olle Soder
BACKGROUND: Prenatal events such as intrauterine growth restriction can affect gonadal development of the offspring and have an impact on reproductive health. PURPOSE: To investigate the effects of intrauterine growth restricted induced by uterine artery ligation on the postnatal rat testis. METHODS: Pregnant rats underwent uterine artery ligation at day 19 of gestation. Offspring were sacrificed at 5, 20 and 40 days post-partum (dpp). At sacrifice, one gonad was snap frozen in liquid nitrogen and processed for RNA and steroid extraction...
November 24, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27884205/whole-genome-sequence-analysis-of-serum-amino-acid-levels
#12
Bing Yu, Paul S de Vries, Ginger A Metcalf, Zhe Wang, Elena V Feofanova, Xiaoming Liu, Donna Marie Muzny, Lynne E Wagenknecht, Richard A Gibbs, Alanna C Morrison, Eric Boerwinkle
BACKGROUND: Blood levels of amino acids are important biomarkers of disease and are influenced by synthesis, protein degradation, and gene-environment interactions. Whole genome sequence analysis of amino acid levels may establish a paradigm for analyzing quantitative risk factors. RESULTS: In a discovery cohort of 1872 African Americans and a replication cohort of 1552 European Americans we sequenced exons and whole genomes and measured serum levels of 70 amino acids...
November 24, 2016: Genome Biology
https://www.readbyqxmd.com/read/27883132/microfluidic-pdms-on-paper-pop-devices
#13
Jin-Wen Shangguan, Yu Liu, Jian-Bin Pan, Bi-Yi Xu, Jing-Juan Xu, Hong-Yuan Chen
In this paper, we propose a generalized concept of microfluidic polydimethylsiloxane (PDMS) on paper (POP) devices, which combines well the merits of paper chips and PDMS chips. First, we optimized the conditions for accurate PDMS spatial patterning on paper, based on screen printing and a high temperature enabled superfast curing technique, which enables PDMS patterning to an accuracy of tens of microns in less than ten seconds. This, in turn, makes it available for seamless, reversible and reliable integration of the resulting paper layer with other PDMS channel structures...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27882481/assessment-criteria-for-vitamin-d-deficiency-insufficiency-in-japan-proposal-by-an-expert-panel-supported-by-the-research-program-of-intractable-diseases-ministry-of-health-labour-and-welfare-japan-the-japanese-society-for-bone-and-mineral-research-and-the
#14
Ryo Okazaki, Keiichi Ozono, Seiji Fukumoto, Daisuke Inoue, Mika Yamauchi, Masanori Minagawa, Toshimi Michigami, Yasuhiro Takeuchi, Toshio Matsumoto, Toshitsugu Sugimoto
Vitamin D is indispensable for the maintenance of bone and mineral health. Inadequate vitamin D action increases the risk for various musculoskeletal/mineral events including fracture, fall, secondary hyperparathyroidism, diminished response to antiresorptives, rickets/osteomalacia, and hypocalcemia. Its most common cause in recent years is vitamin D deficiency/insufficiency, clinically defined by a low serum 25-hydroxyvitamin D [25(OH)D] level. Guidelines for vitamin D insufficiency/deficiency defined by serum 25(OH)D concentrations have been published all over the world...
November 23, 2016: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/27881135/cat-s-whiskers-orthosiphon-stamineus-tea-modulates-arthritis-pathogenesis-via-the-angiogenesis-and-inflammatory-cascade
#15
Yasser M Tabana, Fouad Saleih R Al-Suede, Mohamed B Khadeer Ahamed, Saad S Dahham, Loiy E Ahmed Hassan, Saba Khalilpour, Mohamad Taleb-Agha, Doblin Sandai, Aman S Abdul Majid, Amin Malik Shah Abdul Majid
BACKGROUND: Orthosiphon stamineus is used traditionally to treat gout, arthritis, and inflammatory related conditions. The in vitro anti-inflammatory effects of the plant have been scientifically investigated. The goal of the present study was to evaluate the potential of the 50% ethanol extract of O. stamineus (EOS) to treat rheumatoid arthritis. METHODS: Anti-arthritic activity was assessed using the in vitro heat denaturation test and the (FCA)-induced arthritis model...
November 24, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27880979/low-dose-lipopolysaccharide-causes-biliary-injury-by-blood-biliary-barrier-impairment-in-a-rat-hepatic-ischemia-reperfusion-model
#16
J Reiling, K R Bridle, M Gijbels, F G Schaap, L Jaskowski, N Santrampurwala, L J Britton, C M Campbell, S W M Olde Damink, D H G Crawford, C H C Dejong, J Fawcett
INTRODUCTION: This study explored whether bacterial endotoxins, in the form of lipopolysaccharides (LPS), could have an injurious effect on the biliary tract in conjunction with ischemia. METHOD: Sixty-four rats were randomly assigned to four groups: sham operation (sham), 1mg/kg LPS i.p. (LPS), hepatic ischemia reperfusion (IR), and IR combined with LPS (IR+LPS). Following 1 or 6 hours of reperfusion, serum liver tests, bile duct histology, immunofluorescence microscopy (zonula occludens-1), bile composition (bile salts, phospholipids, lactate dehydrogenase), hepatic gene expression (bile salt transporters and inflammatory mediators) as well as serum and biliary cytokine concentrations were quantified and compared between the study groups...
November 23, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/27879161/neopterin-and-oxidative-stress-markers-in-the-diagnosis-of-extrapulmonary-tuberculosis
#17
Nisha Goyal, Bineeta Kashyap, N P Singh, Iqbal R Kaur
BACKGROUND: Extrapulmonary tuberculosis (EPTB) often presents with non-specific signs and symptoms. Further the paucibacillary nature of extrapulmonary specimens and irregular distribution of bacilli lower the sensitivity of conventional diagnostic methods making EPTB, a diagnostic dilemma. Objective To study neopterin, protein carbonyl and malondialdehyde in extrapulmonary tuberculosis. METHODS: 69 clinically confirmed cases with an equal number of age and sex matched healthy controls were enrolled...
November 23, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/27870915/effects-of-angiopoietin-2-on-transplanted-mouse-ovarian-tissue
#18
Hye Won Youm, Jaewang Lee, Eun Jung Kim, Hyun Sun Kong, Jung Ryeol Lee, Chang Suk Suh, Seok Hyun Kim
Transplantation of ovarian tissue (OT) is currently the only clinical option to restore fertility with cryopreserved OT. However, follicle loss caused by ischemia and slow revascularization occurs in transplanted OT. To shorten the ischemic period and promote angiogenesis, some angiogenic factors have been used. Angiopoietin-2 (Ang2) is one of the major angiogenic factors and has been reported to promote blood vessels and increase vascular permeability in ischemic and/or hypoxic environment. This study was performed to investigate the effects of Ang2 on follicle integrity and revascularization of transplanted mouse OT...
2016: PloS One
https://www.readbyqxmd.com/read/27869251/an-increased-genotoxic-risk-in-lymphocytes-from-phototherapy-treated-hyperbilirubinemic-neonates
#19
Seyed Alireza Mesbah-Namin, Maryam Shahidi, Maryam Nakhshab
BACKGROUND: Phototherapy is believed to be a safe method for the management of hyperbilirubinemia. However, there are some controversial issues regarding the genotoxic effects of phototherapy on DNA. The aim of this study was to investigate morphologically both phototherapy-induced DNA double-strand breaks (DSBs) and apoptosis in lymphocytes derived from jaundiced and non-jaundiced neonates. METHODS: Newborns were divided into three groups, including phototherapy-treated (PT, n=30) jaundiced newborns with total serum bilirubin (TSB) levels >15 mg/dl, non-treated jaundiced newborns (C+, n=27), as positive, as well as healthy negative (C-, n=30) controls with TSB levels ranging from 10 and 15 mg/dl and less than 5 mg/dl, respectively...
November 21, 2016: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/27869176/quantitative-secretomic-analysis-of-pancreatic-cancer-cells-in-serum-containing-conditioned-medium
#20
Peng Liu, Yejing Weng, Zhigang Sui, Yunhao Wu, Xiangli Meng, Mengwei Wu, Haoyi Jin, Xiaodong Tan, Lihua Zhang, Yukui Zhang
Pancreatic cancer is a highly metastatic and chemo-resistant disease. Secreted proteins involved in cell-cell interactions play an important role in changing the tumor microenvironment. Previous studies generally focus on the secretome of cancer cell line from serum-free media, due to the serious interference of fetal bovine serum (FBS). However, serum-starvation may alter expression patterns of secreted proteins. Hence, efforts to decrease the interference of serum in proteomic analysis of serum-containing media have been hampered to quantitatively measure the tumor secretion levels...
November 21, 2016: Scientific Reports
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