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https://www.readbyqxmd.com/read/29350477/pharmacokinetic-evaluation-and-safety-of-topical-1-morphine-sulfate-application-on-the-healthy-equine-eye
#1
Emma Gordon, Bernadette V Stang, Jerry Heidel, Keith P Poulsen, Christopher K Cebra, John W Schlipf
OBJECTIVE: To determine if corneal epithelial cell integrity is detrimentally affected by short-term administration of 1.0% morphine sulfate. Additionally, we sought to determine if topical 1.0% morphine applied to the equine cornea would result in ocular or systemic absorption. ANIMAL STUDIED: Six healthy horses. PROCEDURE: Morphine sulfate (1.0%) was applied topically to one eye every four hours for 72 h before horses were euthanized. Serum samples were collected at varying time points during the study and aqueous and vitreous humor were collected immediately after euthanasia...
January 19, 2018: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/29348564/inhibition-of-wnt-%C3%AE-catenin-signaling-under-serum-starvation-and-hypoxia-induces-adipocytic-transdifferentiation-in-human-leiomyoma-cells
#2
Hiroshi Harada, Yojiro Tsuda, Kei Yabuki, Eisuke Shiba, Kazuyoshi Uchihashi, Atsuji Matsuyama, Yoshihisa Fujino, Toru Hachisuga, Masanori Hisaoka
Fatty metamorphosis is an uncommon alteration in uterine leiomyoma (i.e., lipoleiomyoma), and the pathogenetic mechanisms underlying this phenomenon remain poorly understood. Because a conditional deletion of β-catenin, a major transducer of the canonical Wingless/integrated (WNT) pathway, in the developing mouse uterus can induce adipogenesis in the myometrium, it is hypothesized that inhibition of the WNT/β-catenin signaling may be also involved in the development of fat cells within uterine leiomyoma. In the current study, which was performed to address this point, intracytoplasmic lipid droplets were detectable in cultured human leiomyoma cells by treatment with a potent tankyrase inhibitor, XAV939, which antagonizes β-catenin, in a serum-starved culture medium without additional adipogenesis-inducing agents or supplements, and showed increasing accumulation in a time-dependent manner...
January 18, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29346416/decoding-the-network-of-trypanosoma-brucei-proteins-that-determines-sensitivity-to-apolipoprotein-l1
#3
Rachel B Currier, Anneli Cooper, Hollie Burrell-Saward, Annette MacLeod, Sam Alsford
In contrast to Trypanosoma brucei gambiense and T. b. rhodesiense (the causative agents of human African trypanosomiasis), T. b. brucei is lysed by apolipoprotein-L1 (apoL1)-containing human serum trypanolytic factors (TLF), rendering it non-infectious to humans. While the mechanisms of TLF1 uptake, apoL1 membrane integration, and T. b. gambiense and T. b. rhodesiense apoL1-resistance have been extensively characterised, our understanding of the range of factors that drive apoL1 action in T. b. brucei is limited...
January 18, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29346026/a-scintigraphic-method-for-quantitation-of-lymphatic-function-in-arm-lymphedema
#4
Svend Hvidsten, Navid M Toyserkani, Jens A Sørensen, Poul F Høilund-Carlsen, Jane A Simonsen
BACKGROUND: Lymphoscintigraphy is commonly used to assess breast cancer-related lymphedema. However, a reliable quantitative method that clearly distinguishes normal lymphatic function from lymphedema is desired. We propose a quantitative method based upon the physiological mean transit time (MTT) measure of lymph fluid passing through the arm. METHODS AND RESULTS: Eleven patients, aged 34-68 years, with unilateral arm lymphedema following breast cancer treatment underwent simultaneous bilateral lymphoscintigraphy using intradermal injection of 99mTc-labeled human serum albumin (HSA)...
January 18, 2018: Lymphatic Research and Biology
https://www.readbyqxmd.com/read/29345158/the-impact-of-probiotics-and-n-3-long-chain-polyunsaturated-fatty-acids-on-intestinal-permeability-in-pregnancy-a-randomised-clinical-trial
#5
K Mokkala, P Pussinen, N Houttu, E Koivuniemi, T Vahlberg, K Laitinen
A disruption in intestinal barrier integrity may predispose individuals to metabolic aberrations, particularly during the vulnerable period of pregnancy. We investigated whether intestinal permeability, as measured by serum zonulin concentration, changes over the duration of pregnancy and whether this change is reflected in lipopolysaccharide (LPS) activity. Second, we tested in a randomised double-blind placebo controlled clinical trial the impact of consuming dietary probiotics and/or long chain polyunsaturated fatty acid (LC-PUFA) supplements in lowering serum zonulin concentration and LPS activity...
January 18, 2018: Beneficial Microbes
https://www.readbyqxmd.com/read/29344813/stroke-volume-variation-and-serum-creatinine-changes-during-abdominal-aortic-aneurysm-surgery-a-time-integrated-analysis
#6
Paolo Lentini, Luca Zanoli, Pasquale Fatuzzo, Faeq Husain-Syed, Rudi Stramanà, Diego Cognolato, Vincenzo Catena, Marco Baiocchi, Antonio Granata, Roberto Dell'Aquila
BACKGROUND: Patients undergoing abdominal aortic aneurysm (AAA) surgery with suprarenal clamping are at high risk for acute kidney injury (AKI) and major cardiac and cerebrovascular events (MACCE). We aimed to assess whether the stroke volume variation (SVV), a measure of hemodynamic instability, is associated with AKI in hypertensive patients undergoing elective AAA surgery with suprarenal clamping. METHODS: In a cohort of 51 hypertensive patients, we performed serial measurements of SVV (n = 459) and serum creatinine (sCr) (n = 255)...
January 17, 2018: Journal of Nephrology
https://www.readbyqxmd.com/read/29338319/clinical-evaluation-of-circulating-mir-548a-3p-and-20a-expression-in-malignant-pleural-mesothelioma-patients
#7
Marwa Matboli, Ayman E Shafei, Ahmed Em Azazy, Maged Reda, Nashwa El-Khazragy, Ahmed Aly Nagy, Mahmoud A Ali, Mohamed Sobhi, Omar Abdel-Rahman
AIM: miRNAs may act as promising diagnostic and prognostic biomarkers of mesothelioma. This study integrates serum  miR-548a-3p and miR-20a expression based on in silico data analysis followed by clinical validation in malignant mesothelioma patients (malignant pleural mesothelioma [MPM]). PATIENTS & METHODS: Serum miR-548a-3p and  miR-20a level was assessed in the serum of patients with MPM, chronic asbestos exposure and healthy volunteers by quantitative real-time PCR...
January 17, 2018: Biomarkers in Medicine
https://www.readbyqxmd.com/read/29335857/effect-of-soothing-gan-liver-and-invigorating-pi-spleen-recipes-on-tlr4-p38-mapk-pathway-in-kupffer-cells-of-non-alcoholic-steatohepatitis-rats
#8
Xiang-Wen Gong, Yong-Jian Xu, Qin-He Yang, Yin-Ji Liang, Yu-Pei Zhang, Guan-Long Wang, Yuan-Yuan Li
OBJECTIVE: To investigate the mechanism of inflammatory-mediated toll-like receptor 4 (TLR4)-p38 mitogen-activated protein kinase (p38 MAPK) pathway in Kupffer cells (KCs) of non-alcoholic steatohepatitis (NASH) rats and the intervention effect of soothing Gan (Liver) and invigorating Pi (Spleen) recipes on this pathway. METHODS: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table (n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder (, CHSG) group (3...
January 9, 2018: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29333041/pharmacological-evaluation-of-hepatoprotective-activity-of-ahpl-aytab-0613-tablet-in-carbon-tetrachloride-ethanol-and-paracetamol-induced-hepatotoxicity-models-in-wistar-albino-rats
#9
Sanjay U Nipanikar, Sohan S Chitlange, Dheeraj Nagore
Background: Hepatotoxicity ultimately leads to liver failure. Conventional treatment options for hepatotoxicity are limited and not safe. Objective: Formulation AHPL/AYTAB/0613 is developed to provide safer and effective hepatoprotective drug of natural origin. A study was conducted to evaluate hepatoprotective activity of AHPL/AYTAB/0613 (three dosages) in comparison with marketed formulations in carbon tetrachloride (CCl4), ethanol, and paracetamol-induced hepatotoxicity in Wistar albino rats...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29332825/development-of-gd3-immobilized-glutathione-coated-magnetic-nanoparticles-for-highly-selective-enrichment-of-phosphopeptides
#10
Dandan Jiang, Xiqian Li, Jiutong Ma, Qiong Jia
In this study, we designed a gadolinium-based immobilized metal ion affinity chromatography material for the selective enrichment of phosphopeptides. Gadolinium ion was immobilized on the surface of glutathione-coated magnetic nanoparticles through a facile and effective synthetic route. The adsorbent integrated the advantages of superparamagnetism of Fe3O4 core, good biological compatibility of glutathione, and strong interaction between gadolinium ion and phosphopeptides. It was employed to enrich phosphopeptides from standard protein digests coupled with MALDI-TOF MS...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331900/a-catalytic-and-dual-recycling-amplification-atp-sensor-based-on-target-driven-allosteric-structure-switching-of-aptamer-beacons
#11
Ying Peng, Daxiu Li, Ruo Yuan, Yun Xiang
Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331430/disposable-inkjet-printed-electrochemical-platform-for-detection-of-clinically-relevant-her-2-breast-cancer-biomarker
#12
Susanita Carvajal, Samantha N Fera, Abby L Jones, Thaisa A Baldo, Islam M Mosa, James F Rusling, Colleen E Krause
Rapidly fabricated, disposable sensor platforms hold tremendous promise for point-of-care detection. Here, we present an inexpensive (< $0.25) fully inkjet printed electrochemical sensor with integrated counter, reference, and working electrodes that is easily scalable for commercial fabrication. The electrochemical sensor platform featured an inkjet printed gold working 8-electrode array (WEA) and counter electrode (CE), along with an inkjet -printed silver electrode that was chlorinated with bleach to produce a Ag/AgCl quasi-reference electrode (RE)...
January 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331429/paper-based-fluorescent-sensor-via-aggregation-induced-emission-fluorogen-for-facile-and-sensitive-visual-detection-of-hydrogen-peroxide-and-glucose
#13
Jiafu Chang, Haiyin Li, Ting Hou, Wenna Duan, Feng Li
Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is related to the oxidative stress in organisms, and plays important roles in a variety of cellular activities as well. So it is of crucial importance to develop sensitive and accurate sensing strategies to detect H2O2 in biological systems. Herein, by taking advantage of the unique emission characteristics of aggregation induced emission (AIE) fluorogens, we proposed a non-enzymatic fluorescence platform for facile and sensitive detection of H2O2, both in solution state using fluorescence spectrometer and on paper-based sensor via visual inspection...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29330025/the-role-of-extracellular-and-intracellular-nicotinamide-phosphoribosyl-transferase-in-cancer-diagnostic-and-therapeutic-perspectives-and-challenges
#14
REVIEW
Maria Dalamaga, Gerasimos Socrates Christodoulatos, Christos S Mantzoros
Nicotinamide phosphoribosyl-transferase (Nampt) or pre-B cell colony-enhancing factor or visfatin represents a pleiotropic molecule acting as an enzyme, a cytokine and a growth factor. Intracellular Nampt plays an important role in cellular bioenergetics and metabolism, particularly NAD biosynthesis. NAD biosynthesis is critical in DNA repair, oncogenic signal transduction, transcription, genomic integrity and apoptosis. Although its insulin-mimetic function remains a controversial issue, extracellular Nampt presents proliferative, anti-apoptotic, pro-inflammatory, pro-angiogenic and metastatic properties...
January 9, 2018: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29325893/stilamin-inhibits-intestinal-and-pancreatic-injury-in-rats-with-severe-acute-pancreatitis-by-down-regulating-lcn2-expression
#15
Yifan Ren, Qing Cui, Jianbin Bi, Zhaoqing Du, Jia Zhang, Xufeng Zhang, Yi Lv, Rongqian Wu
OBJECTIVE: This study was aimed at exploring the function and mechanism of Stilamin on severe acute pancreatitis (SAP) in rats. METHODS: We analyzed the histology of small intestine and pancreatic tissues of SAP rats and SAP rats plus treatment with Stilamin. The expression levels of IL-6 and TNF-α in serum were detected using ELISA. The expressions of Caspase-3, bcl-2, Bax and p53 in rat tissues of SAP group were verified by qRT-PCR and western blot. The differential expressed mRNAs in rats of SAP group were screened out by microarray analysis...
January 2, 2018: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/29322779/mining-the-secretome-of-c2c12-muscle-cells-data-dependent-experimental-approach-to-analyze-protein-secretion-using-label-free-quantification-and-peptide-based-analysis
#16
Leonie Grube, Rafael Dellen, Fabian Kruse, Holger Schwender, Kai Stuehler, Gereon Poschmann
Secretome analysis faces several challenges, including detection of low abundant proteins and the discrimination of bona fide secreted proteins from false-positive identifications stemming from cell leakage or serum. Here, we developed a two-step secretomics approach and applied it to the analysis of secreted proteins of C2C12 skeletal muscle cells since the skeletal muscle has been identified as an important endocrine organ secreting myokines as signaling molecules. First, we compared culture supernatants with corresponding cell lysates by mass spectrometry-based proteomics and label-free quantification...
January 11, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29321569/xeno-free-pre-vascularized-spheroids-for-therapeutic-applications
#17
E Bauman, T Feijão, D T O Carvalho, P L Granja, C C Barrias
Spheroid culture has gained increasing popularity, arising as a promising tool for regenerative medicine applications. Importantly, spheroids may present advantages over single-cell suspensions in cell-based therapies (CT). Unfortunately, most growth media used for spheroid culture contain animal origin-components, such as fetal bovine serum (FBS). The presence of FBS compromises the safety of CT and presents economic and ethical constraints. SCC (supplement for cell culture) is a novel xeno-free (XF) industrial cell culture supplement, derived from well-controlled pooled human plasma and processed under good manufacturing practice rules...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321553/prediction-of-co-expression-genes-and-integrative-analysis-of-gene-microarray-and-proteomics-profile-of-keshan-disease
#18
Sen Wang, Rui Yan, Bin Wang, Peiru Du, Wuhong Tan, Mikko J Lammi, Xiong Guo
Keshan disease (KD) is a kind of endemic cardiomyopathy which has a high mortality. However, molecular mechanism in the pathogenesis of KD remains poorly understood. Serum samples were collected from 112 KD patients and 112 normal controls. Gene microarray was used to screen differently expressed genes. Genevestigator was applied to forecast co-expression genes of significant gene. iTRAQ proteomics analysis was used to verify significant genes and their co-expression genes. GO, COG, IPA and STRING were applied to undertake function categorization, pathway and network analysis separately...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321335/serum-hepatocyte-growth-factor-is-probably-associated-with-3-month-prognosis-of-acute-ischemic-stroke
#19
Zhengbao Zhu, Tan Xu, Daoxia Guo, Xinfeng Huangfu, Chongke Zhong, Jingyuan Yang, Aili Wang, Chung-Shiuan Chen, Yanbo Peng, Tian Xu, Jinchao Wang, Yingxian Sun, Hao Peng, Qunwei Li, Zhong Ju, Deqin Geng, Jing Chen, Yonghong Zhang, Jiang He
BACKGROUND AND PURPOSE: Serum hepatocyte growth factor (HGF) is positively associated with poor prognosis of heart failure and myocardial infarction, and it can also predict the risk of ischemic stroke in population. The goal of this study was to investigate the association between serum HGF and prognosis of ischemic stroke. METHODS: A total of 3027 acute ischemic stroke patients were included in this post hoc analysis of the CATIS (China Antihypertensive Trial in Acute Ischemic Stroke)...
January 10, 2018: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/29320517/explaining-the-decline-in-coronary-heart-disease-mortality-rates-in-the-slovak-republic-between-1993-2008
#20
Marek Psota, Piotr Bandosz, Eva Gonçalvesová, Mária Avdičová, Mária Bucek Pšenková, Martin Studenčan, Jarmila Pekarčíková, Simon Capewell, Martin O'Flaherty
OBJECTIVE: Between the years 1993 and 2008, mortality rates from coronary heart disease (CHD) in the Slovak Republic have decreased by almost one quarter. However, this was a smaller decline than in neighbouring countries. The aim of this modelling study was therefore to quantify the contributions of risk factor changes and the use of evidence-based medical therapies to the CHD mortality decline between 1993 and 2008. METHODS: We identified, obtained and scrutinised the data required for the model...
2018: PloS One
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