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Serum integrity

Matthew T Koroscil, Andrew Skabelund
Introduction: Blastomyces dermatitidis is a dimorphic fungus endemic to the Mississippi River valley. We describe a rare case of chronic pulmonary blastomycosis complicated by large pulmonary cavitation in a young service member who was misdiagnosed with active pulmonary tuberculosis. Case Presentation: A 25-year-old active duty male presented to his primary care provider with complaints of hemoptysis, fatigue, weight loss, and fever. Computed tomography chest with contrast identified a large cavitary lesion in the right upper lobe (RUL)...
March 14, 2018: Military Medicine
Naomi Weintrob, Anita Schachter Davidov, Anat Segev Becker, Galit Israeli, Asaf Oren, Ori Eyal
OBJECTIVE: The total cortisol (TC) response may be measured during the glucagon stimulation test (GST) for growth hormone (GH) reserve in order to assess the integrity of the hypothalamic-pituitary-adrenal (HPA) axis. Measurements of TC are unreliable in conditions of albumin and cortisol-binding globulin (CBG) alterations (e.g., hypoproteinemia or CBG deficiency). We aimed to measure the serum free cortisol (sFC) response to the GST in children and adolescents and determine whether it could predict the GH response to glucagon stimulation...
March 2018: Endocrine Practice
Alejandra Nieto, Holger Roehl
There has been a growing interest in recent years in the assessment of suitable vial/stopper combinations for storage and shipment of frozen drug products. Considering that the glass transition temperature (Tg) of butyl rubber stoppers used in Container Closure Systems (CCS) is between -55°C to -65°C, a storage or shipment temperature of a frozen product below the Tg of the rubber stopper, may require special attention, since below the Tg the rubber becomes more plastic-like and loses its elastic (sealing) characteristics...
March 15, 2018: PDA Journal of Pharmaceutical Science and Technology
Margarita Villar, Lourdes Mateos-Hernandez, Jose de la Fuente
BACKGROUND: Why an autoimmune disease that is the main cause of the acute neuromuscular paralysis worldwide has not yet a well-characterized cause or an effective treatment? The existence of different clinical variants for the Guillain-Barré syndrome (GBS) coupled with the fact that a high number of pathogens can cause an infection that sometimes, but not always, precedes the development of the syndrome, confers a high degree of uncertainty for both prognosis and treatment. In the post-genomic era, the development of omics technologies for the high-throughput analysis of biological molecules is allowing the characterization of biological systems in a degree of depth unimaginable before...
March 14, 2018: Current Medicinal Chemistry
Alfredo Perales-Puchalt, Jairo Perez-Sanz, Kyle K Payne, Nikolaos Svoronos, Michael J Allegrezza, Ricardo A Chaurio, Carmen Anadon, Joseph Calmette, Subir Biswas, Jessica A Mine, Tara Lee Costich, Logan Nickels, Jayamanna Wickramasinghe, Melanie R Rutkowski, Jose R Conejo-Garcia
Due to their cytotoxic activities, many anticancer drugs cause extensive damage to the intestinal mucosa and have antibiotic activities. Here, we show that cisplatin induces significant changes in the repertoire of intestinal commensal bacteria that exacerbate mucosal damage. Restoration of the microbiota through fecal-pellet gavage drives healing of cisplatin-induced intestinal damage. Bacterial translocation to the blood stream is correspondingly abrogated, resulting in a significant reduction in systemic inflammation, as evidenced by decreased serum IL-6 and reduced mobilization of granulocytes...
March 14, 2018: Journal of Leukocyte Biology
Katharina Moser, Stefanie Mitze, Erik Teske, Wolf von Bomhard, Christian Stockhaus
OBJECTIVE: The objective of this study was to correlate clinical signs and diagnostic parameters with duodenal inflammatory and architectural changes in dogs with lymphocytic-plasmacytic enteropathy. MATERIAL AND METHODS: In a retrospective study dogs presented between 2003 and 2014 with chronic gastrointestinal signs (duration > 3 weeks) and histologic evidence of intestinal lymphocytic-plasmacytic inflammation were evaluated. Clinical signs, serum albumin, cobalamin and folic acid concentrations were recorded and a sonographic, endoscopic, histologic and cytological inflammatory score was determined...
February 2018: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
Salma Oktaria, Norma Sofisa Hurif, Wardiansyah Naim, Hok Bing Thio, Tamar E C Nijsten, Jan Hendrik Richardus
BACKGROUND: Poverty has long been considered a risk factor for leprosy and is related to nutritional deficiencies. In this study, we aim to investigate the association between poverty-related diet and nutrition with leprosy. METHODOLOGY/PRINCIPAL FINDINGS: In rural leprosy-endemic areas in Indonesia, we conducted a household-based case-control study using two controls for each case patient (100 recently diagnosed leprosy patients and 200 controls), matched for age and gender...
March 13, 2018: PLoS Neglected Tropical Diseases
Daria Tretiakova, Natalia Onishchenko, Ivan Boldyrev, Ilya Mikhalyov, Alexander Tuzikov, Nicolai Bovin, Evgeniy Evtushenko, Elena Vodovozova
Previously, we proposed a liposomal formulation of melphalan (Mlph)-a chemotherapeutic alkylating agent-incorporated in a fluid lipid bilayer in the form of dioleoylglyceride ester. In this work, we compared the stabilizing effect of different amphiphiles included in the Mlph-liposomes, such as phosphatidylinositol (PI), ganglioside GM1 , a conjugate of N-carboxymethyl-modified oligoglycine with dioleoylphosphatidylethanolamine (acidic lipopeptide), and polyethylene glycol (2000 Da) conjugated with dipalmitoylphosphatidylethanolamine (PEG-lipid), upon incubation in human serum...
March 1, 2018: Colloids and Surfaces. B, Biointerfaces
David Parker, Edward Walter, Gareth Williams
A series of three europium(III) complexes has been created in which an APTRA moiety has been integrated into the sensitising chromophore (APTRA = o-aminophenol-N,N,N-triacetate). The constitutionally isomeric complexes EuL1 and EuL2 feature the APTRA unit linked to a metal-bound pyridine ring through an alkynyl unit, differing according to the disposition of the APTRA substituents relative to the C≡C unit (para-N and para-O respectively). In EuL3, the APTRA ring is directly bonded to the Eu-coordinated pyridine (para-O)...
March 13, 2018: Chemistry: a European Journal
Caoileann H Murphy, Mahalakshmi Shankaran, Tyler A Churchward-Venne, Cameron J Mitchell, Nathan M Kolar, Louise M Burke, John A Hawley, Amira Kassis, Leonidas G Karagounis, Kelvin Li, Chelsea King, Marc Hellerstein, Stuart M Phillips
We determined how the pattern of protein intake and resistance training (RT) influenced longer-term (2-wk) integrated myofibrillar protein synthesis (MyoPS) during energy restriction (ER). MyoPS and proteome kinetics were measured during 2-wk of ER alone and 2-wk of ER plus RT (ER + RT) in overweight/obese older men. Participants were randomized to consume dietary protein in a balanced (BAL: 25% daily protein/meal x 4 meals) or skewed (SKEW: 7:17:72:4% daily protein/meal) pattern (n = 10/group). Participants ingested D2 O during the consecutive 2-wk periods, and skeletal muscle biopsies and serum were obtained at the beginning and conclusion of ER and ER + RT...
March 12, 2018: Journal of Physiology
Xuming Sun, Lin Huang, Ru Zhang, Wei Xu, Jingyi Huang, Deepanjali D Gurav, Vadanasundari Vedarethinam, Ruoping Chen, Jiatao Lou, Qian Wang, Jingjing Wan, Kun Qian
Current metabolic analysis is far from ideal to engage clinics and needs rationally designed materials and device. Here we developed a novel plasmonic chip for clinical metabolic fingerprinting. We first constructed a series of chips with gold nanoshells on the surface through controlled particle synthesis, dip-coating, and gold sputtering for mass production. We integrated the optimized chip with microarrays for laboratory automation and micro-/nanoscaled experiments, which afforded direct high-performance metabolic fingerprinting by laser desorption/ionization mass spectrometry using 500 nL of various biofluids and exosomes...
February 28, 2018: ACS Central Science
Nusrat S Shommu, Craig N Jenne, Jaime Blackwood, Ari R Joffe, Dori-Ann Martin, Graham C Thompson, Hans J Vogel
Various limitations hinder the timely and accurate diagnosis of appendicitis in pediatric patients. The present study aims to investigate the potential of metabolomics and cytokine profiling for improving the diagnosis of pediatric appendicitis. Serum and plasma samples were collected from pediatric patients for metabolic and inflammatory mediator analyses respectively. Targeted metabolic profiling was performed using Proton Nuclear Magnetic Resonance Spectroscopy and Flow Injection Analysis Mass Spectrometry/Mass Spectrometry and targeted cytokine/chemokine profiling was completed using a multiplex platform to compare children with and without appendicitis...
2018: PloS One
Katja M Gist, David S Cooper, Julia Wrona, Sarah Faubel, Christopher Altmann, Zhiqian Gao, Bradley S Marino, Jeffrey Alten, Kristal M Hock, Tomoyuki Mizuno, Alexander A Vinks, Melanie S Joy, Michael F Wempe, Michael R Bennett, Stuart L Goldstein
BACKGROUND: Milrinone, an inotropic agent used ubiquitously in children after cardiac surgery, accumulates in acute kidney injury (AKI). We assessed if urinary AKI biomarkers are predictive of an increase in milrinone concentrations in infants after cardiac surgery. METHODS: Multicenter prospective pilot study of infants undergoing cardiac surgery. Urinary AKI biomarkers were measured in the urine at specific time intervals after cardiopulmonary bypass initiation...
April 2018: Therapeutic Drug Monitoring
Liana Bezerra Dias de Lima, Paula Benevides de Morais, Ricardo Lopes Tortorela de Andrade, Luciana Vieira Mattos, Sandro Estevan Moron
This research aimed to evaluate the ecological risk of xenobiotics associated with agricultural activities by determining metal contents and biomarker responses using tucunaré (Cichla sp.) as a bioindicator. The work was conducted in the southwest region of the state of Tocantins, in the cities of Lagoa da Confusão and Pium. Water samples and specimens of Cichla sp. were collected in the Javaés and Formoso Rivers at three collection points (A, B and C). The concentrations of Cd, Pb, Cu, Cr, Mn, Ni and Zn in water and fish were analyzed...
March 8, 2018: Environmental Pollution
Joseph Wider, Vishnu V R Undyala, Peter Whittaker, James Woods, Xuequn Chen, Karin Przyklenk
Remote ischemic preconditioning (RIPC), the phenomenon whereby brief ischemic episodes in distant tissues or organs render the heart resistant to infarction, has been exhaustively demonstrated in preclinical models. Moreover, emerging evidence suggests that exosomes play a requisite role in conveying the cardioprotective signal from remote tissue to the myocardium. However, in cohorts displaying clinically common comorbidities-in particular, type-2 diabetes-the infarct-sparing effect of RIPC may be confounded for as-yet unknown reasons...
March 9, 2018: Basic Research in Cardiology
Xuejiao Chen, Fuchun Gong, Zhong Cao, Wu Zou, Tingting Gu
Strongly green fluorescent carbon dots (CQDs) have been directly synthesized from 2,4-diaminophenylhydrazine and 2-hydroxy-5-methylisophthalaldehyde through a facile solvothermal method. The novel CQDs exhibit high fluorescence quantum yield and excellent water solubility due to the abundant amino and hydroxy groups on their surface. The use of the as-prepared CQDs combined with Cu2+ constructed a "turn-on" switch cysteine-responsive nanoprobe. In the CQDs-Cu2+ assemblies, the binding of Cu2+ to CQDs results in the fluorescence quenching of CQDs by electron transfer mechanism, while the addition of cysteine leads to the fluorescence recovery because of the competitive binding between cysteine and CQDs to Cu2+ ...
March 9, 2018: Analytical and Bioanalytical Chemistry
Chenyue Ding, Qinyan Zou, Fuxin Wang, Huihua Wu, Rulei Chen, Jinghuan Lv, Mingfa Ling, Jian Sun, Wei Wang, Hong Li, Boxian Huang
BACKGROUND: Although many reports show that various kinds of stem cells have the ability to recover function in premature ovarian aging, few studies have looked at stem cell treatment of natural ovarian aging (NOA). We designed this experimental study to investigate whether human amniotic mesenchymal stem cells (hAMSCs) retain the ability to restore ovarian function, and how hAMSCs work in this process. METHODS: To build the NOA mouse model, the mice were fed for 12-14 months normally with young fertile female mice as the normal control group (3-5 months old)...
March 9, 2018: Stem Cell Research & Therapy
Alma L Saucedo-Yanez, Marlene M Perales-Quintana, David Paniagua-Vega, Concepcion Sanchez-Martinez, Paula Cordero-Perez, Noemi Waskman Minsky
Chronic kidney disease (CKD) is a progressive condition characterized by a permanent and irreversible loss of renal function. In accordance to international guidelines, CKD clinical diagnosis methods are based on creatinine and albumin levels and glomerular filtration rate. Unfortunately, these parameters are scarcely affected in early stages, and its inherent intrinsic variability only allows for the identification of intermediate and advanced stages, when life expectancy has become shorter and treatment poses a significant financial investment...
March 6, 2018: Current Medicinal Chemistry
Aleksandra Żebrowska, Barbara Hall, Aleksandra Kochańska-Dziurowicz, Grażyna Janikowska
BACKGROUND: An integral part of the treatment of diabetes is physical activity. Scientific reports have shown the beneficial effects of hypoxia and exercise on cardiovascular and metabolic variables in patients with diabetes. OBJECTIVES: The aim of the study was to assess the effect of normobaric hypoxia and exercise on the serum concentrations of proangiogenic factors and glycemia in patients with type 1 diabetes. MATERIAL AND METHODS: A total of 28 adults (aged 30...
February 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Katie A O'Brien, Ross D Pollock, Mike Stroud, Rob J Lambert, Alex Kumar, Robert A Atkinson, David A Green, Ana Anton-Solanas, Lindsay M Edwards, Steve D R Harridge
An insufficient supply of oxygen to the tissues (hypoxia), as is experienced upon high-altitude exposure, elicits physiological acclimatization mechanisms alongside metabolic remodeling. Details of the integrative adaptive processes in response to chronic hypobaric hypoxic exposure remain to be sufficiently investigated. In this small applied field study, subjects (n = 5, male, age 28-54 years) undertook a 40 week Antarctica expedition in the winter months, which included 24 weeks residing above 2500 m...
March 2018: Physiological Reports
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