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tangential flow filtration

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https://www.readbyqxmd.com/read/29684561/a-pilot-study-using-a-novel-pyrotechnically-driven-prototype-applicator-for-epidermal-powder-immunization-in-piglets
#1
Julia Engert, Cihad Anamur, Laura Engelke, Christian Fellner, Peter Lell, Stefan Henke, Julia Stadler, Susanne Zöls, Mathias Ritzmann, Gerhard Winter
Epidermal powder immunization (EPI) is an alternative technique to the classical immunization route using needle and syringe. In this work, we present the results of an in vivo pilot study in piglets using a dried influenza model vaccine which was applied by EPI using a novel pyrotechnically driven applicator. A liquid influenza vaccine (Pandemrix® ) was first concentrated by tangential flow filtration and hemagglutinin content was determined by RP-HPLC. The liquid formulation was then transformed into a dry powder by collapse freeze-drying and subsequent cryo-milling...
April 20, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29561890/physical-modeling-of-vortical-cross-step-flow-in-the-american-paddlefish-polyodon-spathula
#2
Hannah Brooks, Grant E Haines, M Carly Lin, S Laurie Sanderson
Vortical cross-step filtration in suspension-feeding fish has been reported recently as a novel mechanism, distinct from other biological and industrial filtration processes. Although crossflow passing over backward-facing steps generates vortices that can suspend, concentrate, and transport particles, the morphological factors affecting this vortical flow have not been identified previously. In our 3D-printed models of the oral cavity for ram suspension-feeding fish, the angle of the backward-facing step with respect to the model's dorsal midline affected vortex parameters significantly, including rotational, tangential, and axial speed...
2018: PloS One
https://www.readbyqxmd.com/read/29553879/optimizing-the-preparation-and-stability-of-decorated-antiretroviral-drug-nanocrystals
#3
Tian Zhou, Zhiyi Lin, Pavan Puligujja, Diana Palandri, James Hilaire, Mariluz Araínga, Nathan Smith, Nagsen Gautam, JoEllyn McMillan, Yazen Alnouti, Xinming Liu, Benson Edagwa, Howard E Gendelman
AIM: While the  therapeutic potential for current long-acting (LA) antiretroviral therapy (ART) is undeniable, ligand-decorated nanoformulated LA-ART could optimize drug delivery to viral reservoirs. The development of decorated ART hinges, however, on formulation processes and manufacture efficiencies. To this end, we compared manufacture and purification techniques for ligand-decorated antiretroviral drug nanocrystals. MATERIALS & METHODS: Ligand-decorated nanoparticle manufacturing was tested using folic acid (FA) nanoformulated cabotegravir...
March 19, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29535850/scalable-cgmp-compatible-purification-of-extracellular-vesicles-carrying-bioactive-human-heterodimeric-il-15-lactadherin-complexes
#4
Dionysios C Watson, Bryant C Yung, Cristina Bergamaschi, Bhabadeb Chowdhury, Jenifer Bear, Dimitris Stellas, Aizea Morales-Kastresana, Jennifer C Jones, Barbara K Felber, Xiaoyuan Chen, George N Pavlakis
The development of extracellular vesicles (EV) for therapeutic applications is contingent upon the establishment of reproducible, scalable, and high-throughput methods for the production and purification of clinical grade EV. Methods including ultracentrifugation (U/C), ultrafiltration, immunoprecipitation, and size-exclusion chromatography (SEC) have been employed to isolate EV, each facing limitations such as efficiency, particle purity, lengthy processing time, and/or sample volume. We developed a cGMP-compatible method for the scalable production, concentration, and isolation of EV through a strategy involving bioreactor culture, tangential flow filtration (TFF), and preparative SEC...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29510657/tangential-flow-filtration-technique-an-overview-on-nanomedicine-applications
#5
Teresa Musumeci, Antonio Leonardi, Angela Bonaccorso, Rosario Pignatello, Giovanni Puglisi
Purification is a key step for different types of approaches, ranging from food, biotechnology to pharmaceutical fields. In biotechnology, tangential flow filtration (TFF) allows obtaining the separation of different components of cells without instability phenomena. In the food industry, TFF ensures the removal of contaminants or other substances that negatively affect visual appearance, organoleptic attributes, nutritional value and/or safety of ailments. In the pharmaceutical area, purification is also an important and necessary step controlling the quality of final product...
2018: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/29411865/purification-characterization-and-n-glycosylation-of-recombinant-butyrylcholinesterase-from-transgenic-rice-cell-suspension-cultures
#6
Jasmine M Corbin, Muchena J Kailemia, C Linn Cadieux, Salem Alkanaimsh, Kalimuthu Karuppanan, Raymond L Rodriguez, Carlito B Lebrilla, Douglas M Cerasoli, Karen A McDonald, Somen Nandi
Recombinant butyrylcholinesterase produced in a metabolically regulated transgenic rice cell culture (rrBChE) was purified to produce a highly pure (95%), active form of enzyme. The developed downstream process uses common manufacturing friendly operations including tangential flow filtration, anion-exchange chromatography, and affinity chromatography to obtain a process recovery of 42% active rrBChE. The purified rrBChE was then characterized to confirm its comparability to the native human form of the molecule (hBChE)...
May 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29314774/leveraging-single-pass-tangential-flow-filtration-to-enable-decoupling-of-upstream-and-downstream-monoclonal-antibody-processing
#7
Alex Brinkmann, Sanaa Elouafiq, John Pieracci, Matthew Westoby
Decoupling upstream and downstream operations in biopharmaceutical production could enable more flexible manufacturing operations and could allow companies to leverage strategic or financial benefits that would be otherwise unattainable. A decoupling process was developed and scaled up utilizing single-pass tangential flow filtration for volume reduction, followed by bulk freezing in single-use bags prior to purification. Single-pass tangential flow filtration can be used to continuously concentrate harvested cell culture fluid, reducing the volume by 15-25× with a step yield of >96%...
January 5, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29281809/wastewater-characterisation-by-combining-size-fractionation-chemical-composition-and-biodegradability
#8
Kristin T Ravndal, Eystein Opsahl, Andrea Bagi, Roald Kommedal
The potential for resource recovery from wastewater can be evaluated based on a detailed characterisation of wastewater. In this paper, results from fractionation and characterisation of two distinct wastewaters are reported. Using tangential flow filtration, the wastewater was fractionated into 10 size fractions ranging from 1 kDa to 1 mm, wherein the chemical composition and biodegradability were determined. Carbohydrates were dominant in particulate size fractions larger than 100 μm, indicating a potential of cellulose recovery from these fractions...
December 18, 2017: Water Research
https://www.readbyqxmd.com/read/29152858/in-vitro-evaluation-of-anti-aggregation-and-degradation-behavior-of-pegylated-polymeric-nanogels-under-in-vivo-like-conditions
#9
Yinan Chen, George R Dakwar, Kevin Braeckmans, Twan Lammers, Wim E Hennink, Josbert M Metselaar
The in vivo stability and biodegradability of nanocarriers crucially determine therapeutic efficacy as well as safety when used for drug delivery. This study aims to evaluate optimized in vitro techniques predictive for in vivo nanocarrier behavior. Polymeric biodegradable nanogels based on hydroxyethyl methacrylamide-oligoglycolates-derivatized poly(hydroxyethyl methacrylamide-co-N-(2-azidoethyl)methacrylamide) and with various degrees of PEGylation and crosslinking densities are prepared. Three techniques are chosen and refined for specific in vitro evaluation of the nanocarrier performance: (1) fluorescence single particle tracking (fSPT) to study the stability of nanogels in human plasma, (2) tangential flow filtration (TFF) to study the degradation and filtration of nanogel degradation products, and (3) fluorescence fluctuation spectroscopy (FFS) to evaluate and compare the degradation behavior of nanogels in buffer and plasma...
January 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29076623/inflammation-stimulated-mesenchymal-stromal-cell-derived-extracellular-vesicles-attenuate-inflammation
#10
Matthew T Harting, Amit K Srivastava, Siqin Zhaorigetu, Henry Bair, Karthik S Prabhakara, Naama E Toledano Furman, Jody V Vykoukal, Katherine A Ruppert, Charles S Cox, Scott D Olson
Extracellular vesicles (EVs) secreted by mesenchymal stromal cells (MSCs) have been proposed to be a key mechanistic link in the therapeutic efficacy of cells in response to cellular injuries through paracrine effects. We hypothesize that inflammatory stimulation of MSCs results in the release of EVs that have greater anti-inflammatory effects. The present study evaluates the immunomodulatory abilities of EVs derived from inflammation-stimulated and naive MSCs (MSCEv+ and MSCEv, respectively) isolated using a current Good Manufacturing Practice-compliant tangential flow filtration system...
January 2018: Stem Cells
https://www.readbyqxmd.com/read/29043350/microfluidic-diafiltration-on-chip-using-an-integrated-magnetic-peristaltic-micropump
#11
Jessica F Liu, Sagar Yadavali, Andrew Tsourkas, David Issadore
Diafiltration is a membrane filtration technique that rapidly removes permeable molecules from a solution by controlling the tangential and orthogonal flows over a membrane and by replenishing the permeate with an equivalent amount of replacement buffer. However, its application to the purification of many key biomaterials and nanomaterials has been limited by the large dead volume (>10 mL) that is required to automate the process. To address this challenge, we have developed a diafiltration-on-a-chip device that can process low-volume samples (50 μL)...
November 7, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28992119/a-reproducible-and-scalable-procedure-for-preparing-bacterial-extracts-for-cell-free-protein-synthesis
#12
Kazushige Katsura, Takayoshi Matsuda, Yuri Tomabechi, Mayumi Yonemochi, Kazuharu Hanada, Noboru Ohsawa, Kensaku Sakamoto, Chie Takemoto, Mikako Shirouzu
Cell-free protein synthesis is a useful method for preparing proteins for functional or structural analyses. However, batch-to-batch variability with regard to protein synthesis activity remains a problem for large-scale production of cell extract in the laboratory. To address this issue, we have developed a novel procedure for large-scale preparation of bacterial cell extract with high protein synthesis activity. The developed procedure comprises cell cultivation using a fermentor, harvesting and washing of cells by tangential flow filtration, cell disruption with high-pressure homogenizer and continuous diafiltration...
November 1, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28840656/effect-of-zinc-chloride-and-peg-concentrations-on-the-critical-flux-during-tangential-flow-microfiltration-of-bsa-precipitates
#13
Zhao Li, Andrew L Zydney
There is renewed interest in the possibility of using precipitation for initial capture of high value therapeutic proteins as part of an integrated continuous downstream process. These precipitates can be continuously washed using tangential flow filtration, with long term operation achieved by operating the membrane modules below the critical filtrate flux for fouling. Our hypothesis was that the critical flux for the precipitated protein would be a function of the properties of the precipitate as determined by the precipitation conditions...
November 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28828646/sequential-filtration-a-gentle-method-for-the-isolation-of-functional-extracellular-vesicles
#14
Mitja L Heinemann, Jody Vykoukal
A prevalent challenge in isolating extracellular vesicles (EVs) from biological fluids is the reliable depletion of abundant contaminants-including free proteins and biomolecules, as well as nontarget vesicle subpopulations and other nanoparticulates-from the sample matrix while maximizing recovery. Sequential Filtration is a recently published approach for the size-based isolation of exosomes that is ideally suited for large-volume biofluid samples such as ascites , urine , lavage fluid, or cell-conditioned media...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28776064/manufacturing-of-proteins-and-antibodies-chapter-downstream-processing-technologies-harvest-operations
#15
Richard Turner, Adrian Joseph, Nigel Titchener-Hooker, Jean Bender
Cell harvesting is the separation or retention of cells and cellular debris from the supernatant containing the target molecule Selection of harvest method strongly depends on the type of cells, mode of bioreactor operation, process scale, and characteristics of the product and cell culture fluid. Most traditional harvesting methods use some form of filtration, centrifugation, or a combination of both for cell separation and/or retention. Filtration methods include normal flow depth filtration and tangential flow microfiltration...
August 4, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28751635/microfluidic-cell-retention-device-for-perfusion-of-mammalian-suspension-culture
#16
Taehong Kwon, Holly Prentice, Jonas De Oliveira, Nyasha Madziva, Majid Ebrahimi Warkiani, Jean-François P Hamel, Jongyoon Han
Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28648546/process-intensification-for-high-yield-production-of-influenza-h1n1-gag-virus-like-particles-using-an-inducible-hek-293-stable-cell-line
#17
Alina Venereo-Sanchez, Melanie Simoneau, Stéphane Lanthier, Parminder Chahal, Lucie Bourget, Sven Ansorge, Rénald Gilbert, Olivier Henry, Amine Kamen
Influenza virus dominant antigens presentation using virus like particle (VLP) approach is attractive for the development of new generation of influenza vaccines. Mammalian cell platform offers many advantages for VLP production. However, limited attention has been paid to the processing of mammalian cell produced VLPs. Better understanding of the production system could contribute to increasing the yields and making large-scale VLP vaccine manufacturing feasible. In a previous study, we have generated a human embryonic kidney HEK-293 inducible cell line expressing Hemagglutinin (HA) and Neuraminidase (NA), which was used to produce VLPs upon transient transfection with a plasmid containing HIV-1 Gag...
June 22, 2017: Vaccine
https://www.readbyqxmd.com/read/28645718/optimizing-adjuvants-for-intradermal-delivery-of-menc-glycoconjugate-vaccine
#18
Agnese Donadei, Cristiana Balocchi, Maria R Romano, Luigi Panza, Roberto Adamo, Francesco Berti, Derek T O'Hagan, Simona Gallorini, Barbara C Baudner
Intradermal vaccine delivery is a promising alternative to the conventional intramuscular route. The skin layer is immunologically supported by a densely network of antigen presenting cells, while the skeletal muscle is loaded with a relatively sparse population of APCs. Nevertheless, the vaccine to be suitable for intradermal delivery needs a new formulation to facilitate either smaller injection volumes or the introduction into new delivery devises as micro-needles. This study presents a proof of concept for intradermal delivery of the MenC-CRM197 glycoconjugate vaccine using a mouse model...
July 13, 2017: Vaccine
https://www.readbyqxmd.com/read/28480535/integrated-continuous-bioprocessing-economic-operational-and-environmental-feasibility-for-clinical-and-commercial-antibody-manufacture
#19
James Pollock, Jon Coffman, Sa V Ho, Suzanne S Farid
This paper presents a systems approach to evaluating the potential of integrated continuous bioprocessing for monoclonal antibody (mAb) manufacture across a product's lifecycle from preclinical to commercial manufacture. The economic, operational, and environmental feasibility of alternative continuous manufacturing strategies were evaluated holistically using a prototype UCL decisional tool that integrated process economics, discrete-event simulation, environmental impact analysis, operational risk analysis, and multiattribute decision-making...
July 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28464237/ultrafiltration-behavior-of-monoclonal-antibodies-and-fc-fusion-proteins-effects-of-physical-properties
#20
Youngbin Baek, Nripen Singh, Abhiram Arunkumar, Michael Borys, Zheng J Li, Andrew L Zydney
Ultrafiltration (UF) is used for the final concentration and formulation of essentially all antibody-based therapeutics including both monoclonal antibodies (mAbs) and Fc-fusion proteins. The objective of this study was to quantitatively compare the filtrate flux behavior for two highly purified mAbs and an Fc-fusion protein under identical flow and buffer conditions. Filtrate flux data were obtained using a Pellicon 3 tangential flow filtration cassette over a wide range of transmembrane pressures and bulk protein concentrations...
September 2017: Biotechnology and Bioengineering
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