keyword
https://read.qxmd.com/read/32970854/the-detection-of-isochromosome-i-12p-in-malignant-germ-cell-tumours-and-tumours-with-somatic-malignant-transformation-using-qpcr
#21
JOURNAL ARTICLE
Alexander Fichtner, Annika Richter, Simon Filmar, Nadine T Gaisa, Stefan Schweyer, Henning Reis, Daniel Nettersheim, Christoph Oing, Fabian A Gayer, Andreas Leha, Stefan Küffer, Philipp Ströbel, Silke Kaulfuß, Felix Bremmer
Malignant germ cell tumours (GCT) of the testis are rare neoplasms, but the most common solid malignancy of young men. WHO guidelines divide GCT into five types for which numerous immunohistochemical markers allow the exact histological subtyping in the majority of the cases. In contrast, the germ cell origin is often hard to prove in metastatic GCT that have developed so-called somatic malignant transformation. A high percentage of up to 89% of germ cell tumours are characterized by the appearance of an isochromosome 12p (i[12p])...
September 24, 2020: Histopathology
https://read.qxmd.com/read/32907838/ezh2-regulates-pancreatic-cancer-subtype-identity-and-tumor-progression-via-transcriptional-repression-of-gata6
#22
JOURNAL ARTICLE
Shilpa Patil, Benjamin Steuber, Waltraut Kopp, Vijayalakshmi Kari, Laura Urbach, Xin Wang, Stefan Küffer, Hanibal Bohnenberger, Dimitra Spyropoulou, Zhe Zhang, Lennart Versemann, Mark Sebastian Bösherz, Marius Brunner, Jochen Gaedcke, Philipp Ströbel, Jin-San Zhang, Albrecht Neesse, Volker Ellenrieder, Shiv K Singh, Steven A Johnsen, Elisabeth Hessmann
Recent studies have thoroughly described genome-wide expression patterns defining molecular subtypes of pancreatic ductal adenocarcinoma (PDAC) with different prognostic and predictive implications. Although the reversible nature of key regulatory transcription circuits defining the two extreme PDAC subtype lineages "classical" and "basal-like" suggests that subtype states are not permanently encoded but underlie a certain degree of plasticity, pharmacologically actionable drivers of PDAC subtype identity remain elusive...
September 9, 2020: Cancer Research
https://read.qxmd.com/read/32814982/prognostic-impact-of-additional-hpv-diagnostics-in-102-patients-with-p16-stratified-advanced-oropharyngeal-squamous-cell-carcinoma
#23
JOURNAL ARTICLE
Bernhard G Weiss, Mahalia Zoe Anczykowski, Stefan Küffer, Jennifer L Spiegel, Mattis Bertlich, Martin Canis, Friedrich Ihler, Julia Kitz, Mark Jakob
PURPOSE: p16 overexpression was considered as surrogate marker to identify human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma (OPSCCs). METHODS: 102 patients with advanced stage OPSCCs treated primarily by transoral lasermicrosurgery were included. Prognostic associations of p16- and HPV-status were analyzed separately and combined. RESULTS: In contrast to p16, the HPV-status resulted in no significant survival discrepancies (5-year overall survival (OS) HPV-positive 64...
August 20, 2020: European Archives of Oto-rhino-laryngology
https://read.qxmd.com/read/32588101/role-of-cancer-stem-cell-markers-aldh1-bcl11b-bmi-1-and-cd44-in-the-prognosis-of-advanced-hnscc
#24
JOURNAL ARTICLE
Mark Jakob, Kariem Sharaf, Markus Schirmer, Martin Leu, Stefan Küffer, Mattis Bertlich, Friedrich Ihler, Frank Haubner, Martin Canis, Julia Kitz
PURPOSE: Cancer stem cells (CSCs) are held accountable for the progress of head and neck squamous cell carcinoma (HNSCC). In the presented study, the authors evaluated the prognostic value of CSC markers in two particular HNSCC cohorts. METHODS: This two cohort study consisted of 85 patients with advanced stage HNSCC, treated with primary radio(chemo)therapy (pRCT), and 95 patients with HNSCC, treated with surgery and partially adjuvant radio(chemo)therapy. Overall survival (OS), disease-free survival (DFS), and disease-specific survival (DSS) were assessed...
March 2021: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://read.qxmd.com/read/31565104/proteomic-comparison-of-malignant-human-germ-cell-tumor-cell-lines
#25
JOURNAL ARTICLE
Felix Bremmer, Hanibal Bohnenberger, Stefan Küffer, Thomas Oellerich, Hubert Serve, Henning Urlaub, Arne Strauss, Yasmine Maatoug, Carl Ludwig Behnes, Christoph Oing, Heinz Joachim Radzun, Philipp Ströbel, Stefan Balabanov, Friedemann Honecker
Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal carcinoma-like cell line, were compared by a quantitative proteomic approach using high-resolution mass spectrometry (MS) in combination with stable isotope labelling by amino acid in cell culture (SILAC)...
2019: Disease Markers
https://read.qxmd.com/read/31401665/potentially-actionable-fgfr2-high-level-amplification-in-thymic-sebaceous-carcinoma
#26
JOURNAL ARTICLE
Stefan Porubsky, Peter Jessup, Damien Kee, Rajiv Sharma, Ayame Ochi, Huiling Xu, Jens J Froelich, Louise Nott, Clare Scott, Raef Awad, Cristina Moldovan, Ashutosh A Hardikar, Hanibal Bohnenberger, Stefan Küffer, Philipp Ströbel, Alexander Marx
Our aim was to investigate sebaceous differentiation in thymus tumours and to identify new actionable genomic alterations. To this end we screened 35 normal and 23 hyperplastic thymuses, 127 thymomas and 41 thymic carcinomas for the presence of sebaceous differentiation as defined by morphology and expression of adipophilin and androgen receptor (AR). One primary thymic carcinoma showed morphology of sebaceous carcinomas (keratinizing and foam cells, calcifications, giant cells), a strong expression of adipophilin and AR together with squamous markers...
August 10, 2019: Virchows Archiv: An International Journal of Pathology
https://read.qxmd.com/read/31355988/microrna-expression-patterns-in-oral-squamous-cell-carcinoma-hsa-mir-99b-3p-and-hsa-mir-100-5p-as-novel-prognostic-markers-for-oral-cancer
#27
JOURNAL ARTICLE
Mark Jakob, Lena M Mattes, Stefan Küffer, Kristian Unger, Julia Hess, Mattis Bertlich, Frank Haubner, Friedrich Ihler, Martin Canis, Bernhard G Weiss, Julia Kitz
BACKGROUND: MicroRNAs (miRNA) recently evolved as potential cancer biomarkers. Therefore, the aim of the present study was to evaluate the prognostic impact of eight miRNAs connected to oral squamous cell carcinoma (OSCC). METHOD: Expression levels of hsa-mir-21-5p, hsa-mir-29b-3p, hsa-mir-31-5p, hsa-mir-99a-5p, hsa-mir-99b-3p, hsa-mir-100-5p, hsa-mir-143-3p and hsa-mir-155-5p were analyzed in tumor tissue (n = 36) and healthy oral mucosal tissue (n = 17) and correlated with clinical variables...
July 29, 2019: Head & Neck
https://read.qxmd.com/read/31050844/ewsr1-translocation-in-primary-hyalinising-clear-cell-carcinoma-of-the-thymus
#28
JOURNAL ARTICLE
Stefan Porubsky, Birgit Rudolph, Jens-Carsten Rückert, Stefan Küffer, Philipp Ströbel, Anja C Roden, Deepali Jain, Thomas Tousseyn, Hans Van Veer, James Huang, Alberto Antonicelli, Tseng-Tong Kuo, Juan Rosai, Alexander Marx
AIMS: In thymic carcinomas, focal clear cell change is a frequent finding. In addition to a prominent, diffuse clear cell morphology, some of these carcinomas show an exuberant hyalinised extracellular matrix, and therefore probably represent a separate entity. However, a characteristic genomic alteration remains elusive. We hypothesised that, analogous to hyalinising clear cell carcinomas of the salivary gland, hyalinising clear cell carcinomas of the thymus might also harbour EWSR1 translocations...
September 2019: Histopathology
https://read.qxmd.com/read/31042566/molecular-classification-of-neuroendocrine-tumors-of-the-thymus
#29
JOURNAL ARTICLE
Helen Dinter, Hanibal Bohnenberger, Julia Beck, Kirsten Bornemann-Kolatzki, Ekkehard Schütz, Stefan Küffer, Lukas Klein, Teri J Franks, Anja Roden, Alexander Emmert, Marc Hinterthaner, Mirella Marino, Luka Brcic, Helmut Popper, Cleo-Aron Weis, Giuseppe Pelosi, Alexander Marx, Philipp Ströbel
INTRODUCTION: The WHO classification of pulmonary neuroendocrine tumors (PNETs) is also used to classify thymic NETs (TNETs) into typical and atypical carcinoid (TC and AC), large cell neuroendocrine carcinoma (LCNEC), and small cell carcinoma (SCC), but little is known about the usability of alternative classification systems. METHODS: One hundred seven TNET (22 TC, 51 AC, 28 LCNEC, and 6 SCC) from 103 patients were classified according to the WHO, the European Neuroendocrine Tumor Society, and a grading-related PNET classification...
August 2019: Journal of Thoracic Oncology
https://read.qxmd.com/read/30181507/expression-of-granulisyn-perforin-and-granzymes-in-human-milk-over-lactation-and-in-the-case-of-maternal-infection
#30
JOURNAL ARTICLE
Alecia-Jane Twigger, Gwendoline K Küffer, Donna T Geddes, Luis Filgueria
Human milk has been previously found to contain various types of leukocytes however specific characteristics of these cells, such as whether they contain cytolytic antimicrobial proteins that may induce pathogen directed cell death, are unknown. This project aims to examine the presence and localization of immune proteins such as perforin, granulysin and granzymes in human milk cells at the protein and mRNA level. Genes encoding these proteins were confirmed in human milk cell samples, which were particularly enriched in early milk and in the case of maternal infection...
September 4, 2018: Nutrients
https://read.qxmd.com/read/30097507/comparative-proteomics-reveals-a-diagnostic-signature-for-pulmonary-head-and-neck-cancer-metastasis
#31
JOURNAL ARTICLE
Hanibal Bohnenberger, Lars Kaderali, Philipp Ströbel, Diego Yepes, Uwe Plessmann, Neekesh V Dharia, Sha Yao, Carina Heydt, Sabine Merkelbach-Bruse, Alexander Emmert, Jonatan Hoffmann, Julius Bodemeyer, Kirsten Reuter-Jessen, Anna-Maria Lois, Leif Hendrik Dröge, Philipp Baumeister, Christoph Walz, Lorenz Biggemann, Roland Walter, Björn Häupl, Federico Comoglio, Kuan-Ting Pan, Sebastian Scheich, Christof Lenz, Stefan Küffer, Felix Bremmer, Julia Kitz, Maren Sitte, Tim Beißbarth, Marc Hinterthaner, Martin Sebastian, Joachim Lotz, Hans-Ulrich Schildhaus, Hendrik Wolff, Bernhard C Danner, Christian Brandts, Reinhard Büttner, Martin Canis, Kimberly Stegmaier, Hubert Serve, Henning Urlaub, Thomas Oellerich
Patients with head-and-neck cancer can develop both lung metastasis and primary lung cancer during the course of their disease. Despite the clinical importance of discrimination, reliable diagnostic biomarkers are still lacking. Here, we have characterised a cohort of squamous cell lung (SQCLC) and head-and-neck (HNSCC) carcinomas by quantitative proteomics. In a training cohort, we quantified 4,957 proteins in 44 SQCLC and 30 HNSCC tumours. A total of 518 proteins were found to be differentially expressed between SQCLC and HNSCC, and some of these were identified as genetic dependencies in either of the two tumour types...
September 2018: EMBO Molecular Medicine
https://read.qxmd.com/read/29709010/amelioration-of-ccl4-induced-liver-injury-in-swiss-albino-mice-by-antioxidant-rich-leaf-extract-of-croton-bonplandianus-baill
#32
JOURNAL ARTICLE
Somit Dutta, Arnab Kumar Chakraborty, Priyankar Dey, Pallab Kar, Pokhraj Guha, Subhajit Sen, Anoop Kumar, Arnab Sen, Tapas Kumar Chaudhuri
The progress in industrialization has blessed mankind with a technologically superior lifestyle but poor management of industrial waste has in turn poisoned nature. One such chemical is carbon tetra chloride (CCl4), which is a potent environmental toxin emitted from chemical industries and its presence in the atmosphere is increasing at an alarming rate. Presence of CCl4 in human body is reported to cause liver damage through free radical mediated inflammatory processes. Kupffer cells present in the liver are potentially more sensitive to oxidative stress than hepatocytes...
2018: PloS One
https://read.qxmd.com/read/29103125/histological-liver-chances-in-swiss-mice-caused-by-tannery-effluent
#33
JOURNAL ARTICLE
Letícia Martins Rabelo, Abraão Tiago Batista Guimarães, Joyce Moreira de Souza, Wellington Alves Mizael da Silva, Bruna de Oliveira Mendes, Raíssa de Oliveira Ferreira, Aline Sueli de Lima Rodrigues, Guilherme Malafaia
Although tannery effluents are known for being highly toxic to organisms, reports about the effects of the intake of these xenobiotics on experimental mammal models are recent. Studies about the damages the chronic intake of these effluents can cause in the liver of outbred mice remain an unexplored field. Thus, the aim of the present study is to assess (histological) the hepatic condition of Swiss mice (outbred strain) chronically exposed to the intake of different raw tannery effluent concentrations diluted in water for 150 days...
January 2018: Environmental Science and Pollution Research International
https://read.qxmd.com/read/28436954/optimized-expression-based-microdissection-of-formalin-fixed-lung-cancer-tissue
#34
JOURNAL ARTICLE
Markus Grafen, Thurid R Hofmann, Andreas H Scheel, Julia Beck, Alexander Emmert, Stefan Küffer, Bernhard C Danner, Ekkehard Schütz, Reinhardt Büttner, Andreas Ostendorf, Philipp Ströbel, Hanibal Bohnenberger
Analysis of specific DNA alterations in precision medicine of tumors is crucially important for molecular targeted treatments. Lung cancer is a prototypic example and one of the leading causes of cancer-related deaths worldwide. One major technical problem of detecting DNA alterations in tissue samples is cellular heterogeneity, that is, mixture of tumor and normal cells. Microdissection is an important tool to enrich tumor cells from heterogeneous tissue samples. However, conventional laser capture microdissection has several disadvantages like user-dependent selection of regions of interest (ROI), high costs for dissection systems and long processing times...
July 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://read.qxmd.com/read/28241754/dna-methylation-profiles-of-elderly-individuals-subjected-to-indentured-childhood-labor-and-trauma
#35
JOURNAL ARTICLE
Zoya Marinova, Andreas Maercker, Andreas Küffer, Mark D Robinson, Tomasz K Wojdacz, Susanne Walitza, Edna Grünblatt, Andrea Burri
BACKGROUND: Childhood trauma is associated with increased vulnerability to mental and somatic disorders later in life. Epigenetic modifications such as DNA methylation are one potential mechanism through which such long-lasting impairments/consequences can be explained. The aim of the present study was to investigate whether childhood trauma is associated with long-term DNA methylation alterations in old age. METHODS: We assessed genome-wide DNA methylation profiles in a cohort of former indentured child laborers ("Verdingkinder") who suffered severe childhood adversities (N = 30; M age = 75...
February 27, 2017: BMC Medical Genetics
https://read.qxmd.com/read/27630582/posttraumatic-stress-disorder-adverse-childhood-events-and-buccal-cell-telomere-length-in-elderly-swiss-former-indentured-child-laborers
#36
JOURNAL ARTICLE
Andreas Lorenz Küffer, Aoife O'Donovan, Andrea Burri, Andreas Maercker
Posttraumatic stress disorder (PTSD) is associated with increased risk for age-related diseases and early mortality. Accelerated biological aging could contribute to this elevated risk. The aim of the present study was to assess buccal cell telomere length (BTL) - a proposed marker of biological age - in men and women with and without PTSD. The role of childhood trauma was assessed as a potential additional risk factor for shorter telomere length. The sample included 62 former indentured Swiss child laborers (age: M = 76...
2016: Frontiers in Psychiatry
https://read.qxmd.com/read/27501152/the-prion-protein-is-an-agonistic-ligand-of-the-g-protein-coupled-receptor-adgrg6
#37
JOURNAL ARTICLE
Alexander Küffer, Asvin K K Lakkaraju, Amit Mogha, Sarah C Petersen, Kristina Airich, Cédric Doucerain, Rajlakshmi Marpakwar, Pamela Bakirci, Assunta Senatore, Arnaud Monnard, Carmen Schiavi, Mario Nuvolone, Bianka Grosshans, Simone Hornemann, Frederic Bassilana, Kelly R Monk, Adriano Aguzzi
Ablation of the cellular prion protein PrP(C) leads to a chronic demyelinating polyneuropathy affecting Schwann cells. Neuron-restricted expression of PrP(C) prevents the disease, suggesting that PrP(C) acts in trans through an unidentified Schwann cell receptor. Here we show that the cAMP concentration in sciatic nerves from PrP(C)-deficient mice is reduced, suggesting that PrP(C) acts via a G protein-coupled receptor (GPCR). The amino-terminal flexible tail (residues 23-120) of PrP(C) triggered a concentration-dependent increase in cAMP in primary Schwann cells, in the Schwann cell line SW10, and in HEK293T cells overexpressing the GPCR Adgrg6 (also known as Gpr126)...
August 25, 2016: Nature
https://read.qxmd.com/read/26708556/myf5-and-myogenin-in-the-development-of-thymic-myoid-cells-implications-for-a-murine-in-vivo-model-of-myasthenia-gravis
#38
JOURNAL ARTICLE
Bo Hu, Katja Simon-Keller, Stefan Küffer, Philipp Ströbel, Thomas Braun, Alexander Marx, Stefan Porubsky
Myasthenia gravis (MG) is caused by autoantibodies against the neuromuscular junction of striated muscle. Most MG patients have autoreactive T- and B-cells directed to the acetylcholine receptor (AChR). To achieve immunologic tolerance, developing thymocytes are normally eliminated after recognition of self-antigen-derived peptides. Presentation of muscle-specific antigens is likely achieved through two pathways: on medullary thymic epithelial cells and on medullary dendritic cells cross-presenting peptides derived from a unique population of thymic myoid cells (TMC)...
March 2016: Experimental Neurology
https://read.qxmd.com/read/26597583/-dynamic-concept-of-oral-lichen-planus-the-diagnosis-easy-at-early-stages-may-become-difficult-in-ancient-lichen-planus
#39
JOURNAL ARTICLE
Tommaso Lombardi, Roger Küffer
Dynamic concept of oral lichen planus. The diagnosis easy at early stages may become difficult in ancient lichen planus. Lichen planus is a chronic inflammatory dermatosis of the skin, skin appendages and mucous membranes, which frequently affects the oral mucosa. Its aetiology still remains unknown, and currently accepted pathogenesis is that of an autoimmune cell-mediated disease. To the contrary of skin lichen planus, oral lichen planus is a long-term chronic disease with dynamic evolution, in which progressive and profound changes of the clinical and histopathological aspects occur over time and under the influence of various exogenous factors...
February 2016: La Presse Médicale
https://read.qxmd.com/read/26451610/role-of-n-cadherin-in-proliferation-migration-and-invasion-of-germ-cell-tumours
#40
JOURNAL ARTICLE
Felix Bremmer, Simon Schallenberg, Hubertus Jarry, Stefan Küffer, Silke Kaulfuss, Peter Burfeind, Arne Strauß, Paul Thelen, Heinz Joachim Radzun, Philipp Ströbel, Friedemann Honecker, Carl Ludwig Behnes
Germ cell tumors (GCTs) are the most common malignancies in young men. Most patients with GCT can be cured with cisplatin-based combination chemotherapy, even in metastatic disease. In case of therapy resistance, prognosis is usually poor. We investigated the potential of N-cadherin inhibition as a therapeutic strategy. We analyzed the GCT cell lines NCCIT, NTERA-2, TCam-2, and the cisplatin-resistant sublines NCCIT-R and NTERA-2R. Effects of a blocking antibody or siRNA against N-cadherin on proliferation, migration, and invasion were investigated...
October 20, 2015: Oncotarget
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