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Plasma mitochondrial dna

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https://www.readbyqxmd.com/read/28641368/glomerular-hyperfiltration-in-obese-african-american-hypertensive-patients-is-associated-with-elevated-urinary-mitochondrial-dna-copy-number
#1
Alfonso Eirin, Ahmed Saad, John R Woollard, Luis A Juncos, David A Calhoun, Hui Tang, Amir Lerman, Stephen C Textor, Lilach O Lerman
BACKGROUND: Glomerular hyperfiltration may contribute to the high incidence of renal disease in Obese African Americans essential hypertensive (ObAAEH) patients, but the precise mechanisms responsible for renal injury have not been elucidated. Mitochondria are important determinants of renal injury in hypertension, and increased levels of mitochondrial DNA (mtDNA) in the urine may indicate renal mitochondrial injury. We hypothesized that urine mtDNA copy numbers would be higher in ObAAEH compared to Caucasian essential hypertensive (CEH) patients...
June 14, 2017: American Journal of Hypertension
https://www.readbyqxmd.com/read/28633757/the-relationship-between-circulating-mitochondrial-dna-and-inflammatory-cytokines-in-patients-with-major-depression
#2
Yuki Kageyama, Takaoki Kasahara, Masaki Kato, Shiho Sakai, Yasuhiko Deguchi, Munehide Tani, Kenji Kuroda, Kotaro Hattori, Sumiko Yoshida, Yuichi Goto, Toshihiko Kinoshita, Koki Inoue, Tadafumi Kato
BACKGROUND: Although inflammatory cytokines are established biomarkers of mood disorders, their molecular mechanism is not known. We hypothesized that circulating mitochondrial DNA (mtDNA) contributes to inflammation and could be used as biomarkers. We investigated if circulating mtDNA level is associated with inflammatory cytokines and can be used as a biomarker of mood disorders. METHODS: Plasma mtDNA concentration was measured with real-time quantitative PCR targeting two regions of the mtDNA and plasma levels of four cytokines (GM-CSF, IL-2, IL-4, and IL-6) were measured with a multiplex immunoassay method in 109 patients with major depressive disorder (MDD)...
June 6, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28626147/depleted-leukocyte-mitochondrial-dna-copy-number-correlates-with-unfavorable-left-ventricular-volumetric-and-spherical-shape-remodeling-in-acute-myocardial-infarction-after-primary-angioplasty
#3
Ching-Hui Huang, Chen-Ling Kuo, Ching-San Huang, Chin-San Liu, Chia-Chu Chang
BACKGROUND: Left ventricular (LV) shape influences LV systolic function. It is possible to assess LV shape using 3-D echocardiography sphericity index (SI). Maintaining mitochondrial DNA copy number (MCN) is important for preserving mitochondrial function and LV systolic function after acute myocardial infarction (AMI). Information is limited, however, regarding the relationship between leukocyte MCN and the subsequent change in LV shape after AMI.Methods and Results:Fifty-five AMI patients undergoing primary angioplasty were recruited...
June 16, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/28619507/sensitivity-of-mitochondrial-dna-depleted-%C3%AF-0-cells-to-h2o2-depends-on-the-plasma-membrane-status
#4
Kazuo Tomita, Yoshikazu Kuwahara, Yuko Takashi, Takao Tsukahara, Akihiro Kurimasa, Manabu Fukumoto, Yoshihiro Nishitani, Tomoaki Sato
To clarify the relationship between mitochondrial DNA (mtDNA)-depleted ρ0 cells and the cellular sensitivity to hydrogen peroxide (H2O2), we established HeLa and SAS ρ0 cell lines and investigated their survival rate in H2O2, radical scavenging enzymes, plasma membrane potential status, and chronological change in intracellular H2O2 amount under the existence of extracellular hydrogen peroxide compared with the parental cells. The results revealed that ρ0 cells had higher sensitivity to H2O2 than their parental cells, even though the catalase activity of ρ0 cells was up-regulated, and the membrane potential of the ρ0 cells was lower than their parental cells...
June 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28612531/-plasma-free-mtdna-and-expression-of-tlr-9-mapk-in-brain-tissues-of-rats-with-subarachnoid-hemorrhage
#5
Jin-Ping Liu, Yong Xia, Jin Li, Chao You
OBJECTIVES: To determine the plasma level of mitochondrial DNA (mtDNA) and the expression of Toll like receptor-9/mitogen-activated protein kinase (TLR-9/MAPK) in the brain tissues of rats with subarachnoid hemorrhage (SAH). METHODS: Sprague-Dawley rats were used to establish SAH models with a modified method of endovascular perforation. Twenty four hours after the establishment of the models, the plasma level of mtDNA in the rats was detected by real-time PCR; the expressions of TLR-9 and P38 MAPK in the brain tissues were detected by reverse transcription PCR (RT-PCR) and immunohistochemical staining...
March 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28600986/neonatal-exposure-to-environmental-pollutants-and-placental-mitochondrial-dna-content-a-multi-pollutant-approach
#6
Annette Vriens, Tim S Nawrot, Willy Baeyens, Elly Den Hond, Liesbeth Bruckers, Adrian Covaci, Kim Croes, Sam De Craemer, Eva Govarts, Nathalie Lambrechts, Ilse Loots, Vera Nelen, Martien Peusens, Stefaan De Henauw, Greet Schoeters, Michelle Plusquin
BACKGROUND: Placental mitochondrial DNA (mtDNA) content can be indicative of oxidative damage to the placenta during fetal development and is responsive to external stressors. In utero exposure to environmental pollutants that may influence placental mtDNA needs further exploration. OBJECTIVES: We evaluated if placental mtDNA content is altered by environmental pollution in newborns and identified pollutants independently associated to alterations in placental mtDNA content...
June 7, 2017: Environment International
https://www.readbyqxmd.com/read/28586364/intracellular-zinc-flux-causes-reactive-oxygen-species-mediated-mitochondrial-dysfunction-leading-to-cell-death-in-leishmania-donovani
#7
Anjali Kumari, Krishn Pratap Singh, Abhishek Mandal, Ranjeet Kumar Paswan, Preeti Sinha, Pradeep Das, Vahab Ali, Sanjiva Bimal, Chandra Shekhar Lal
Leishmaniasis caused by Leishmania parasite is a global threat to public health and one of the most neglected tropical diseases. Therefore, the discovery of novel drug targets and effective drug is a major challenge and an important goal. Leishmania is an obligate intracellular parasite that alternates between sand fly and human host. To survive and establish infections, Leishmania parasites scavenge and internalize nutrients from the host. Nevertheless, host cells presents mechanism like nutrient restriction to inhibit microbial growth and control infection...
2017: PloS One
https://www.readbyqxmd.com/read/28585739/iron-oxide-nanoparticles-induced-cytotoxicity-oxidative-stress-and-dna-damage-in-lymphocytes
#8
Usha Singh Gaharwar, Ramovatar Meena, Paulraj Rajamani
Over the past few decades nanotechnology and material science has progressed extremely rapidly. Iron oxide nanoparticles (IONPs) owing to their unique magnetic properties have a great potential for their biomedical and bioengineering applications. However, there is an inevitable need to address the issue of safety and health effects of these nanoparticles. Hence, the present study was aimed to assess the cytotoxic effects of IONPs on rats' lymphocytes. Using different assays, we studied diverse parameters including mitochondrial membrane potential, intracellular accumulation of reactive oxygen species (ROS), lactate dehydrogenase activity, antioxidant enzymes activity and DNA damage measurements...
June 6, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28554474/the-retraction-of-the-protoplast-during-pcd-is-an-active-and-interruptible-calcium-flux-driven-process
#9
Joanna Kacprzyk, Niall P Brogan, Cara T Daly, Siamsa M Doyle, Mark Diamond, Elizabeth M Molony, Paul F McCabe
The protoplast retracts during apoptosis-like programmed cell death (AL-PCD) and, if this retraction is an active component of AL-PCD, it should be used as a defining feature for this type of programmed cell death. We used an array of pharmacological and genetic tools to test if the rates of protoplast retraction in cells undergoing AL-PCD can be modulated. Disturbing calcium flux signalling, ATP synthesis and mitochondrial permeability transition all inhibited protoplast retraction and often also the execution of the death programme...
July 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28542490/repeated-bouts-of-exhaustive-exercise-increase-circulating-cell-free-nuclear-and-mitochondrial-dna-without-development-of-tolerance-in-healthy-men
#10
Robert Stawski, Konrad Walczak, Piotr Kosielski, Pawel Meissner, Tomasz Budlewski, Gianluca Padula, Dariusz Nowak
OBJECTIVE: Acute single strenuous exercise increases circulating cell free DNA (cf DNA). We tested whether three repeated bouts of exhaustive exercise induced the cf DNA response without development of tolerance in healthy men. METHODS: Eleven average-trained men (age 34.0±5.2 years, body mass index 26.2±3.1 kg/m2, maximal oxygen consumption-VO2max 49.6±4.5 ml/kg*min) performed three treadmill exercise tests to exhaustion at speed corresponding to 70% VO2max separated by 72 hours of resting...
2017: PloS One
https://www.readbyqxmd.com/read/28542447/exploratory-plasma-proteomic-analysis-in-a-randomized-crossover-trial-of-aspirin-among-healthy-men-and-women
#11
Xiaoliang Wang, Ali Shojaie, Yuzheng Zhang, David Shelley, Paul D Lampe, Lisa Levy, Ulrike Peters, John D Potter, Emily White, Johanna W Lampe
Long-term use of aspirin is associated with lower risk of colorectal cancer and other cancers; however, the mechanism of chemopreventive effect of aspirin is not fully understood. Animal studies suggest that COX-2, NFκB signaling and Wnt/β-catenin pathways may play a role, but no clinical trials have systematically evaluated the biological response to aspirin in healthy humans. Using a high-density antibody array, we assessed the difference in plasma protein levels after 60 days of regular dose aspirin (325 mg/day) compared to placebo in a randomized double-blinded crossover trial of 44 healthy non-smoking men and women, aged 21-45 years...
2017: PloS One
https://www.readbyqxmd.com/read/28538623/impact-of-a-novel-pi3-kinase-inhibitor-in-preventing-mitochondrial-dna-damage-and-damage-associated-molecular-pattern-accumulation-results-from-the-biochronicity-project
#12
George E Black, Kyle K Sokol, Donald M Moe, Jon Simmons, David Muscat, Victor Pastukh, Gina Capley, Olena Gorodnya, Mykhalo Ruchko, Mark B Roth, Mark Gillespie, Matthew J Martin
BACKGROUND: Despite improvements in the management of severely injured patients, development of multiple organ dysfunction syndrome (MODS) remains a morbid complication of traumatic shock. One of the key attributes of MODS is a profound bioenergetics crisis, for which the mediators and mechanisms are poorly understood. We hypothesized that metabolic uncoupling using an experimental PI3-kinase inhibitor, LY294002 (LY), may prevent mitochondrial abnormalities that lead to the generation of mitochondrial DNA (mtDNA) damage and the release of mtDNA damage associated molecular patterns (DAMPs) METHODS: 16 swine were studied using LY294002 (LY), a non-selective PI3-KI: Animals were assigned to Trauma only (TO, N=3); LY drug only (LYO, N=3); and Experimental (N=10), trauma + drug (LY+T) groups...
May 22, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28503733/long-term-safety-from-transmission-of-porcine-endogenous-retrovirus-after-pig-to-non-human-primate-corneal-transplantation
#13
Hyuk Jin Choi, Jiyeon Kim, Jae Young Kim, Hyun Ju Lee, Won Ryang Wee, Mee Kum Kim, Eung Soo Hwang
BACKGROUND: The risk of xenozoonosis mainly by porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles in xenotransplantation and therefore should be elucidated prior to the clinical use of porcine corneal grafts. Accordingly, an investigation was performed to analyze the infectivity of PERVs from porcine keratocytes to human cells, and the long-term risk of transmission of PERVs was determined using pig-to-non-human primate (NHP) corneal transplantation models...
May 14, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28487062/mitochondrial-damps-are-released-during-cardiopulmonary-bypass-surgery-and-are-associated-with-postoperative-atrial-fibrillation
#14
Nicola Sandler, Elzbieta Kaczmarek, Kiyoshi Itagaki, Yi Zheng, Leo Otterbein, Kamal Khabbaz, David Liu, Venkatachalam Senthilnathan, Russell L Gruen, Carl J Hauser
BACKGROUND: Atrial fibrillation (AF) is the most frequent complication of surgery performed on cardiopulmonary bypass (CPB) and recent work associates CPB with postoperative inflammation. We have shown that all tissue injury releases mitochondrial damage associated molecular patterns (mtDAMPs) including mitochondrial DNA (mtDNA). This can act as a direct, early activator of neutrophils (PMN), eliciting a systemic inflammatory response syndrome (SIRS) while suppressing PMN function. Neutrophil Extracellular Traps (NETs) are crucial to host defence...
March 24, 2017: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/28479956/a-single-zidovudine-azt-administration-delays-hepatic-cell-proliferation-by-altering-oxidative-state-in-the-regenerating-rat-liver
#15
Armando Butanda-Ochoa, Diego Rolando Hernández-Espinosa, Marisela Olguín-Martínez, Lourdes Sánchez-Sevilla, Mario R Rodríguez, Benito Chávez-Rentería, Alberto Aranda-Fraustro, Rolando Hernández-Muñoz
The 3'-azido-3'-deoxythymidine or Zidovudine (AZT) was the first antiretroviral drug used in the treatment of HIV patients, which has good effectiveness but also hepatotoxic side effects that include cell cycle arrest and oxidative/nitrative mitochondrial damage. Whether such an oxidative damage may affect the proliferative-regenerative capacity of liver remains to be clearly specified at doses commonly used in the clinical practice. In this study, we described the oxidative-proliferative effect of AZT administered at a common clinical dose in rat liver submitted to 70% partial hepatectomy (PH)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28473603/increased-hepatic-mitochondrial-fa-oxidation-leads-to-lower-tg-levels-in-rat-liver-and-plasma-and-is-associated-with-upregulation-of-uncoupling-proteins-and-downregulation-of-apolipoprotein-c-iii
#16
Carine Lindquist, Bodil Bjørndal, Christine Renate Rossmann, Deusdedit Tusubira, Asbjørn Svardal, Gro Vatne Røsland, Karl Johan Tronstad, Seth Hallström, Rolf Kristian Berge
Hepatic mitochondrial function, APOC-III and LPL are potential targets for TG lowering drugs. After three weeks of dietary treatment with the compound 2-(tridec-12 -yn-1-ylthio) acetic acid (1-triple TTA), the hepatic mitochondrial FA oxidation increased more than 5-fold in male Wistar rats. Gene expression analysis in liver showed significant downregulation of APOC-III, and upregulation of LPL and the VLDL receptor. This led to lower hepatic (53 %) and plasma (73 %) TG levels. Concomitantly liver-specific biomarkers related to mitochondrial biogenesis and function (mitochondrial DNA, citrate synthase activity and cytochrome c and Tfam gene expression) were elevated...
May 4, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28469765/melatonin-treatment-enhances-therapeutic-effects-of-exosomes-against-acute-liver-ischemia-reperfusion-injury
#17
Cheuk-Kwan Sun, Chih-Hung Chen, Chia-Lo Chang, Hsin-Ju Chiang, Pei-Hsun Sung, Kuan-Hung Chen, Yi-Ling Chen, Sheng-Yi Chen, Gour-Shenq Kao, Hsueh-Wen Chang, Mel S Lee, Hon-Kan Yip
This study tests the hypothesis that combined melatonin and exogenic adipose mesenchymal stem cell (ADMSC)-derived exosome treatment offers superior protection against liver ischemia-reperfusion (LIR) injury compared to either alone. In vitro studies utilized a macrophage cell line (RAW) pretreated with lipopolysaccharide and hepatocytes pretreated with melatonin or exosomes before hypoxia treatment, while in vitro experiments involved analyses of liver specimens from male adult Sprague-Dawley rats (n = 50) equally categorized into sham controls (SC), LIR only, LIR-exosome (100 µg, 30 minute post-LIR), LIR-melatonin (20 mg/kg, 30 minute post-LIR and 50 mg/kg at 6 and 18 hours post-LIR), and LIR-exosome-melatonin groups...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28464939/circulating-dna-in-rheumatoid-arthritis-pathological-changes-and-association-with-clinically-used-serological-markers
#18
Elena Rykova, Aleksey Sizikov, Dirk Roggenbuck, Oksana Antonenko, Leonid Bryzgalov, Evgeniy Morozkin, Kseniya Skvortsova, Valentin Vlassov, Pavel Laktionov, Vladimir Kozlov
BACKGROUND: Early diagnosis of rheumatoid arthritis (RA) is crucial to providing effective therapy and often hampered by unspecific clinical manifestations. Elevated levels of extracellular circulating DNA (cirDNA) in patients with autoimmune disease were found to be associated with etiopathogenesis. To our knowledge, this is the first study to investigate the putative diagnostic use of cirDNA in RA and its association with disease activity. METHODS: Blood samples were taken from 63 healthy subjects (HS) and 74 patients with RA...
May 2, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28442045/lower-dietary-n-6-n-3-ratio-and-high-dose-vitamin-e-supplementation-improve-sperm-morphology-and-oxidative-stress-in-boars
#19
Qing Liu, Yuanfei Zhou, Runjia Duan, Hongkui Wei, Siwen Jiang, Jian Peng
A 2×2 factorial experiment (10 boars per treatment) was conducted for 16 weeks to evaluate the effects of the dietary n-6:n-3 ratio (14:1 vs 6:1) and vitamin E (200 vs 400mg kg(-1)) on boar sperm morphology and oxidative stress. Sperm mitochondrial membrane potential (MMP), reactive oxygen species (ROS), DNA damage (8-hydroxydeoxyguanosine; 8-OHdG), seminal lipoperoxidation (malondialdehyde; MDA) and antioxidant capacity in the serum, spermatozoa and seminal plasma were assessed as indicators of oxidative stress...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28433809/biocorona-formation-on-gold-nanoparticles-modulates-human-proximal-tubule-kidney-cell-uptake-cytotoxicity-and-gene-expression
#20
M T Ortega, J E Riviere, K Choi, N A Monteiro-Riviere
Gold nanoparticles (AuNP) adsorb macromolecules to form a protein corona (PC) after systemic delivery, to which the kidney as the primary excretory organ is constantly exposed. The role of the PC on AuNP cell uptake and toxicity was investigated in vitro in human proximal tubule cells (HPTC) using 40 and 80nm branched polyethylenimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coated AuNP with or without (bare) PCs composed of human plasma (HP) or human serum albumin (HSA) for 0.25 to 24h. Time-dependent intracellular uptake, assessed by ICP-MS showed PC modulated cell uptake and cytotoxicity; with bare 40nm BPEI-AuNP showing the greatest responses...
April 19, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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