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Plasma mitochondrial dna

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https://www.readbyqxmd.com/read/27933735/danazol-has-potential-to-cause-pkc-translocation-cell-cycle-dysregulation-and-apoptosis-in-breast-cancer-cells
#1
Suman Jyoti Deka, Ashalata Roy, Vibin Ramakrishnan, Debasis Manna, Vishal Trivedi
Danazol, the established clinical drug has given promising therapeutic results in a series of clinical trials with breast cancer patients. Danazol shares structural similarities with several known PKC agonists and fits well into the C1 domain. Danazol binds to the C1b domain of PKC with Kd of 5.64±1.27 μM. MD simulation studies further support that the PKC-Danazol molecular model is stable and showing minimum distortion to the structure during the simulation period. Immunofluorescence and western-blotting studies indicate that MDAMB-231 cells stimulated with danazol exhibit translocation of PKCα to the plasma membrane...
December 9, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27927977/ppar-mrna-levels-are-modified-by-dietary-n-3-fatty-acid-restriction-and-energy-restriction-in-the-brain-and-liver-of-growing-rats
#2
Matthew J Picklo, LuAnn Johnson, Joseph Idso
BACKGROUND: Without dietary sources of n-3 (ω-3) long-chain polyunsaturated fatty acids (LCPUFAs), α-linolenic acid (ALA; 18:3n-3) is the precursor for docosahexaenoic acid (DHA; 22:6n-3). It is not known how energy restriction (ER) affects ALA conversion to DHA. OBJECTIVE: We tested the hypothesis that ER reduces n-3 LCPUFA concentrations in tissues of growing rats fed diets replete with and deficient in ALA. METHODS: Male Sprague-Dawley rats (23 d old) were provided AIN93G diets (4 wk) made with soybean oil (SO; ALA sufficient) or corn oil (CO; ALA deficient) providing 16% of energy as fat...
December 7, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27922635/increased-plasma-levels-of-circulating-cell-free-mitochondrial-dna-in-suicide-attempters-associations-with-hpa-axis-hyperactivity
#3
D Lindqvist, J Fernström, C Grudet, L Ljunggren, L Träskman-Bendz, L Ohlsson, Å Westrin
Preclinical data suggest that chronic stress may cause cellular damage and mitochondrial dysfunction, potentially leading to the release of mitochondrial DNA (mtDNA) into the bloodstream. Major depressive disorder has been associated with an increased amount of mtDNA in leukocytes from saliva samples and blood; however, no previous studies have measured plasma levels of free-circulating mtDNA in a clinical psychiatric sample. In this study, free circulating mtDNA was quantified in plasma samples from 37 suicide attempters, who had undergone a dexamethasone suppression test (DST), and 37 healthy controls...
December 6, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27921220/increased-cell-free-mitochondrial-dna-is-a-marker-of-ongoing-inflammation-and-better-neurocognitive-function-in-virologically-suppressed-hiv-infected-individuals
#4
Josué Pérez-Santiago, Michelli F De Oliveira, Susanna R Var, Tyler R C Day, Steven P Woods, Sara Gianella, Sanjay R Mehta
Cell-free mitochondrial DNA (mtDNA) is a highly immunogenic molecule that is associated with several inflammatory conditions and with neurocognitive impairment during untreated HIV infection. Here, we investigate how cell-free mtDNA in cerebrospinal fluid (CSF) is associated with inflammation, neuronal damage, and neurocognitive functioning in the context of long-term suppressive antiretroviral therapy (ART). We quantified the levels of cell-free mtDNA in the CSF from 41 HIV-infected individuals with completely suppressed HIV RNA levels in blood plasma (<50 copies/mL) by droplet digital PCR...
December 5, 2016: Journal of Neurovirology
https://www.readbyqxmd.com/read/27917263/changes-in-cellular-proliferation-and-plasma-products-are-associated-with-liver-failure
#5
Juliana Gil Melgaço, Frederico Marianetti Soriani, Pedro Henrique Ferreira Sucupira, Leonardo Assaf Pinheiro, Yasmine Rangel Vieira, Jaqueline Mendes de Oliveira, Lia Laura Lewis-Ximenez, Cristina Carvalho Vianna Araújo, Lúcio Filgueiras Pacheco-Moreira, Gustavo Batista Menezes, Oswaldo Gonçalves Cruz, Claudia Lamarca Vitral, Marcelo Alves Pinto
AIM: To study the differences in immune response and cytokine profile between acute liver failure and self-limited acute hepatitis. METHODS: Forty-six patients with self-limited acute hepatitis (AH), sixteen patients with acute liver failure (ALF), and twenty-two healthy subjects were involved in this study. The inflammatory and anti-inflammatory products in plasma samples were quantified using commercial enzyme-linked immunoassays and quantitative real-time PCR...
November 18, 2016: World Journal of Hepatology
https://www.readbyqxmd.com/read/27821553/potential-biomarkers-to-follow-the-progression-and-treatment-response-of-huntington-s-disease
#6
Marie-Hélène Disatnik, Amit U Joshi, Nay L Saw, Mehrdad Shamloo, Blair R Leavitt, Xin Qi, Daria Mochly-Rosen
Huntington's disease (HD) is a rare genetic disease caused by expanded polyglutamine repeats in the huntingtin protein resulting in selective neuronal loss. Although genetic testing readily identifies those who will be affected, current pharmacological treatments do not prevent or slow down disease progression. A major challenge is the slow clinical progression and the inability to biopsy the affected tissue, the brain, making it difficult to design short and effective proof of concept clinical trials to assess treatment benefit...
November 14, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27814627/oxidative-dna-and-mitochondrial-dna-change-in-patients-with-sle
#7
Hui-Ting Lee, Tsai-Hung Wu, Chen-Sung Lin, Chyou-Shen Lee, Siao-Cian Pan, Deh-Ming Chang, Yau-Huei Wei, Chang-Youh Tsai
We evaluated plasma IL-10, IFN-alpha, IL-23, IFN-gamma, IP-10, MCP-1, 8-OHdG, leukocyte mtDNA, serum anti-dsDNA antibodies and disease activity index (SLEDAI) in SLE patients. 93 patients (35 nephritis, 4 under dialysis, 5 under rituximab) and 50 healthy controls were recruited. Compared with healthy controls, SLE patients had higher IL-10, IFN-alpha, IL-23, IFN-gamma, IP-10 and MCP-1 (p<0.05). High IFN-alpha (p=0.031) and IP-10 (p=0.026) correlated with high SLEDAI; high IFN-alpha (p<0.001), IL-23 (p=0...
January 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/27811985/elevated-homocysteine-levels-in-type-2-diabetes-induce-constitutive-neutrophil-extracellular-traps
#8
Manjunath B Joshi, Guruprasad Baipadithaya, Aswath Balakrishnan, Mangala Hegde, Manik Vohra, Rayees Ahamed, Shivashankara K Nagri, Lingadakai Ramachandra, Kapaettu Satyamoorthy
Constitutively active neutrophil extracellular traps (NETs) and elevated plasma homocysteine are independent risk factors for Type 2 Diabetes (T2D) associated vascular diseases. Here, we show robust NETosis due to elevated plasma homocysteine levels in T2D subjects and increased components of NETs such as neutrophil elastase and cell free DNA. Cooperative NETs formation was observed in neutrophils exposed to homocysteine, IL-6 and high glucose suggesting acute temporal changes tightly regulate constitutive NETosis...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27811968/very-short-mitochondrial-dna-fragments-and-heteroplasmy-in-human-plasma
#9
Ruoyu Zhang, Kiichi Nakahira, Xiaoxian Guo, Augustine M K Choi, Zhenglong Gu
Cell free DNA (cfDNA) has received increasing attention and has been studied in a broad range of clinical conditions. However, few studies have focused on mitochondrial DNA (mtDNA) in the cell free form. We optimized DNA isolation and sequencing library preparation protocols to better retain short DNA fragments from plasma, and applied these optimized methods to plasma samples from patients with sepsis. Our methods can retain substantially shorter DNA, resulting in an average of 11.5 fold increase in short DNA fragments yield (DNA <100bp)...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27773698/differential-susceptibility-to-acetaminophen-induced-liver-injury-in%C3%A2-sub-strains-of-c57bl-6-mice-6n-versus-6j
#10
Luqi Duan, John S Davis, Benjamin L Woolbright, Kuo Du, Mala Cahkraborty, James Weemhoff, Hartmut Jaeschke, Mohammed Bourdi
: Mouse models of acetaminophen (APAP) hepatotoxicity are considered relevant for the human pathophysiology. The C57BL/6 strain is most popular because it is the background strain of gene knock-out mice. However, conflicting results in the literature may have been caused by sub-strain mismatches, e.g. C57BL/6J and C57BL/6N. This study was initiated to determine the mechanism behind the sub-strain susceptibility to APAP toxicity. C57BL/6N and C57BL/6J mice were dosed with 200 mg/kg APAP and sacrificed at different time points...
December 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27749098/cytotoxic-effect-of-latanoprost-on-human-corneal-stromal-cells-in-vitro-and-its-possible-mechanisms
#11
Jun-Wei Shen, Ming Shan, Yuan-Yuan Peng, Ting-Jun Fan
PURPOSE: To investigate the cytotoxic effect of latanoprost on corneal stroma and its underlying cellular and molecular mechanisms using non-transfected human corneal stromal (HCS) cells as an in vitro model. METHODS: After HCS cells were treated with latanoprost at concentrations varying from 50 mg/l (clinical therapeutic dosage) to 0.78125 mg/l, and cell morphology, cell viability, and cell cycle were detected by light microscopy, methyl thiazolyl tetrazolium assay, and flow cytometry (FCM) with propidium iodide (PI) staining, respectively...
October 17, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27736919/adjusting-mtdna-quantification-in-whole-blood-for-peripheral-blood-platelet-and-leukocyte-counts
#12
Yamilee Hurtado-Roca, Marta Ledesma, Monica Gonzalez-Lazaro, Raquel Moreno-Loshuertos, Patricio Fernandez-Silva, Jose Antonio Enriquez, Martin Laclaustra
Alterations of mitochondrial DNA copy number (mtDNAcn) in the blood (mitochondrial to nuclear DNA ratio) appear associated with several systemic diseases, including primary mitochondrial disorders, carcinogenesis, and hematologic diseases. Measuring mtDNAcn in DNA extracted from whole blood (WB) instead of from peripheral blood mononuclear cells or buffy coat may yield different results due to mitochondrial DNA present in platelets. The aim of this work is to quantify the contribution of platelets to mtDNAcn in whole blood [mtDNAcn(WB)] and to propose a correction formula to estimate leukocytes' mtDNAcn [mtDNAcn(L)] from mtDNAcn(WB)...
2016: PloS One
https://www.readbyqxmd.com/read/27721319/release-of-mitochondrial-dna-correlates-with-peak-inflammatory-cytokines-in-patients-with-acute-myocardial-infarction
#13
Chaoyi Qin, Jun Gu, Ruiqi Liu, Fei Xu, Hong Qian, Qian He, Wei Meng
OBJECTIVE: The present study is an exploration of the dynamic changes of plasma mitochondrial deoxyribonucleic acid (mtDNA) and inflammatory level in patients with acute myocardial infarction (MI). METHODS: Thirty-eight patients with acute MI and 33 control participants were included in the study. Blood samples were collected on admission, 12 hours post-percutaneous coronary intervention (PCI), 24 hours post-PCI, and 48 hours post-PCI. White blood cell (WBC) count and Creactive protein (CRP) level were determined...
October 5, 2016: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/27720270/use-of-computer-assisted-sperm-analysis-and-flow-cytometry-to-detect-seasonal-variations-of-bovine-semen-quality
#14
E Malama, Y Zeron, F Janett, M Siuda, Z Roth, H Bollwein
Seasonal fluctuations of climate are considered a major factor affecting spermatogenesis and semen quality in the bovine. Our study aimed to investigate the effect of season on functional parameters of frozen-thawed bovine semen using computer-assisted sperm analysis (CASA) and flow cytometry. For this purpose, 86 ejaculates were collected from five mature Holstein-Friesian bulls kept under subtropical conditions during summer (August to September; n = 43) and winter (December to January; n = 43) months. Semen was diluted with a Tris-egg yolk-based extender and frozen at -196 °C...
August 25, 2016: Theriogenology
https://www.readbyqxmd.com/read/27715004/biofilm-impeding-agnps-target-skin-carcinoma-by-inducing-mitochondrial-membrane-depolarization-mediated-through-ros-production
#15
Debasis Nayak, Manisha Kumari, Sripathi Rajachandar, Sarbani Ashe, Neethi Chandra Thathapudi, Bismita Nayak
Reactive oxygen species (ROS) are double edged sword that possesses both beneficial and harmful effects. Although basic research on skin cancer prevention has undergone a huge transformation, cases of recurrence with higher rates of drug resistance are some of its drawbacks. Therefore, targeting mitochondria by ROS overproduction provides an alternate approach for anticancer therapy. In the present study green synthesized silver nanoparticles (AgNPs) were explored for triggering the ROS production in A431 skin carcinoma cells...
October 7, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27693373/ruthenium-clotrimazole-complex-has-significant-efficacy-in-the-murine-model-of-cutaneous-leishmaniasis
#16
Eva Iniguez, Armando Varela-Ramirez, Alberto Martínez, Caresse L Torres, Roberto A Sánchez-Delgado, Rosa A Maldonado
In previous studies we reported a novel series of organometallic compounds, Ru(II) complexed with clotrimazole, displaying potent trypanosomatid activity with unnoticeable toxicity toward normal mammalian cells. In view of the promising activity of Ru-clotrimazole complexes against Leishmania major (L. major), the present work sought to investigate the anti-leishmanial activity of the AM162 complex in the murine model of cutaneous leishmaniasis. In addition, to facilitate the design of new therapeutic strategies against this disease, we investigated the mode of action of two Ru-clotrimazole complexes in L...
December 2016: Acta Tropica
https://www.readbyqxmd.com/read/27688826/altered-striatocerebellar-metabolism-and-systemic-inflammation-in-parkinson-s-disease
#17
Chiun-Chieh Yu, Meng-Hsiang Chen, Cheng-Hsien Lu, Yung-Cheng Huang, Hsiu-Ling Chen, Nai-Wen Tsai, Hung-Chen Wang, I-Hsiao Yang, Shau-Hsuan Li, Wei-Che Lin
Parkinson's disease (PD) is the most second common neurodegenerative movement disorder. Neuroinflammation due to systemic inflammation and elevated oxidative stress is considered a major factor promoting the pathogenesis of PD, but the relationship of structural brain imaging parameters to clinical inflammatory markers has not been well studied. Our aim was to evaluate the association of magnetic resonance spectroscopy (MRS) measures with inflammatory markers. Blood samples were collected from 33 patients with newly diagnosed PD and 30 healthy volunteers...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27680742/protective-effect-of-3-o-methyl-quercetin-and-kaempferol-from-semecarpus-anacardium-against-h2o2-induced-cytotoxicity-in-lung-and-liver-cells
#18
A D Naveen Kumar, Ganesh Babu Bevara, Laxmi Koteswaramma Kaja, Anil Kumar Badana, Rama Rao Malla
BACKGROUND: Hydrogen peroxide is continuously generated in living cells through metabolic pathways and serves as a source of reactive oxygen species. Beyond the threshold level, it damages cells and causes several human disorders, including cancer. METHODS: Effect of isolated 3-O-methyl quercetin and kaempferol on H2O2 induced cytotoxicity, ROS formation, plasma membrane damage, loss of mitochondrial membrane potential, DNA damage was evaluated in normal liver and lung cells...
September 29, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27677465/no-perv-transmission-during-a-clinical-trial-of-pig-islet-cell-transplantation
#19
Vladimir A Morozov, Shaun Wynyard, Shinichi Matsumoto, Adrian Abalovich, Joachim Denner, Robert Elliott
Xenotransplantation of pig islet cells is a promising alternative for the treatment of diabetes with insulin and may help to prevent numerous late complications such as blindness and amputation. First encouraging results using porcine islets have been reported in preclinical animal models as well in the first clinical trial in New Zealand. The goal of this manuscript is to examine the biological safety of a second trial performed in Argentina, specifically in regards to the transmission of porcine endogenous retroviruses (PERVs) using improved detection methods As in the first trial encapsulated islet cells from the well-characterised Auckland Island pigs were used...
January 2, 2017: Virus Research
https://www.readbyqxmd.com/read/27610009/mitochondrial-dna-from-hepatocytes-as-a-ligand-for-tlr9-drivers-of-nonalcoholic-steatohepatitis
#20
EDITORIAL
Priya Handa, Akhila Vemulakonda, Kris V Kowdley, Misael Uribe, Nahum Méndez-Sánchez
Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide, affecting approximately one third of the Western world. It consists of a wide spectrum of liver disorders, ranging from fatty liver to nonalcoholic steatohepatitis (NASH), which consists of steatosis, ballooning injury and inflammation. Despite an alarming growth in the statistics surrounding NAFLD, there are as yet no effective therapies for its treatment. Innate immune signaling has been thought to play a significant role in initiating and augmenting hepatic inflammation, contributing to the transition from nonalcoholic fatty liver to NASH...
August 21, 2016: World Journal of Gastroenterology: WJG
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