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Plasma mitochondrial dna

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https://www.readbyqxmd.com/read/29313215/association-of-mitochondrial-dna-content-in-peripheral-blood-with-cancer-related-fatigue-and-chemotherapy-related-cognitive-impairment-in-early-stage-breast-cancer-patients-a-prospective-cohort-study
#1
Jung-Woo Chae, Peh Siang Chua, Terence Ng, Angie Hui Ling Yeo, Maung Shwe, Yan Xiang Gan, Sreemanee Dorajoo, Koon Mian Foo, Kiley Wei-Jen Loh, Si-Lin Koo, Wen Yee Chay, Tira Jing Ying Tan, Sok Yuen Beh, Elaine Hsuen Lim, Guek Eng Lee, Rebecca Dent, Yoon Sim Yap, Raymond Ng, Han Kiat Ho, Alexandre Chan
PURPOSE: Cancer-related fatigue (CRF) and chemotherapy-related cognitive impairment (CRCI) are reported to be associated with mitochondrial dysfunction. Hence, mitochondrial DNA (mtDNA) content, a biomarker of mitochondrial dysfunction, is hypothesized to correlate with the onset of CRF and CRCI. This study aims to evaluate the association between peripheral blood mtDNA content reduction and severity of CRF and CRCI in patients receiving chemotherapy. METHODS: This was a prospective cohort study...
January 8, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29310422/anti-proliferative-activities-of-vasicinone-on-lung-carcinoma-cells-mediated-via-activation-of-both-mitochondria-dependent-and-independent-pathways
#2
Tapan Dey, Prachurjya Dutta, Prasenjit Manna, Jatin Kalita, Hari Prasanna Deka Boruah, Alak Kumar Buragohain, Balagopalan Unni
Vasicinone, a quinazoline alkaloid from Adhatoda vasica Nees. is well known for its bronchodilator activity. However its anti-proliferative activities is yet to be elucidated. Here-in we investigated the anti-proliferative effect of vasicinone and its underlying mechanism against A549 lung carcinoma cells. The A549 cells upon treatment with various doses of vasicinone (10, 30, 50, 70 μM) for 72 h showed significant decrease in cell viability. Vasicinone treatment also showed DNA fragmentation, LDH leakage, and disruption of mitochondrial potential, and lower wound healing ability in A549 cells...
January 9, 2018: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/29282743/mitochondrial-dna-multiplex-real-time-polymerase-chain-reaction-inhibition-assay-for-quality-control-of-pathogen-inactivation-by-ultraviolet-c-light-in-platelet-concentrates
#3
Sinyoung Kim, Wiebke Handke, Ute Gravemann, Andrea Döscher, Veronika Brixner, Thomas H Müller, Axel Seltsam
BACKGROUND: Several ultraviolet (UV) light-based pathogen inactivation (PI) technologies for platelet (PLT) products have been developed or are under development. Upon implementation of PI technologies, quality control measures are required to ensure consistent efficiency of the treatment process. Previous reports showed that amotosalen/UVA and riboflavin/UV-based PI technologies induce modifications of the PLT-derived mitochondrial DNA (mtDNA) that can be detected by polymerase chain reaction (PCR) inhibition assays...
December 28, 2017: Transfusion
https://www.readbyqxmd.com/read/29248950/a-single-mitochondrial-dna-deletion-accurately-detects-significant-prostate-cancer-in-men-in-the-psa-grey-zone
#4
Jennifer Creed, Laurence Klotz, Andrew Harbottle, Andrea Maggrah, Brian Reguly, Anne George, Vincent Gnanapragasm
PURPOSE: To determine the clinical performance of a blood-based test for clinically significant (CS) prostate cancer (PCa) (grade group ≥ 2) intended for use in men with prostate serum antigen levels in the 'grey zone' (PSA < 10 ng/ml). The test quantifies a previously described 3.4 kb mitochondrial DNA (mtDNA) deletion. METHODS: In a first prospective study of an MRI-guided re-biopsy population (n = 126), the 3.4 kb deletion and 18S rRNA gene were amplified from plasma...
December 16, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/29241162/in-vitro-and-in-vivo-activity-of-fomitopsis-pinicola-sw-ex-fr-karst-chloroform-fpkc-extract-against-s180-tumor-cells
#5
Ye Gao, Pan Wang, Yaqin Wang, Lijie Wu, Xiaobing Wang, Kun Zhang, Quanhong Liu
BACKGROUND/AIMS: Non-toxic fomitopsis is has been traditionally used in folk medicine in many countries for its anti-inflammatory and anti-vascular disease activities. The present study investigates the antitumor effect of Fomitopsis pinicola (Sw. Ex Fr.) Karst chloroform extract (FPKc) on S180 tumor cells in vitro and in vivo and we determined the underlying mechanisms. METHODS: HPLC was employed to analyze the constituents of FPKc. In-vitro 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to quantify the growth inhibition of FPKc; Propidium iodide (PI) exclusion assay and scanning electron microscopy (SEM) were used to observe the damage on the cell membrane and the changes of the cell morphology; Staining with Hoechst 33342/propidium iodide (HO/PI) and the application of the Annexin V-FITC/PI analysis permitted to observe the cell death triggered by FPKc; DNA damage and cell cycle arrest were detected by flow cytometry; Rhodamine 123 (RH123) and Cytochrome C were used as dyes to investigate the alterations of the mitochondria...
December 12, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29236713/sirt3-deficiency-does-not-affect-venous-thrombosis-or-netosis-despite-mild-elevation-of-intracellular-ros-in-platelets-and-neutrophils-in-mice
#6
Hideki Hayashi, Deya Cherpokova, Kimberly Martinod, Thilo Witsch, Siu Ling Wong, Maureen Gallant, Stephen M Cifuni, Leonard P Guarente, Denisa D Wagner
Inflammation is a common denominator in chronic diseases of aging. Yet, how inflammation fuels these diseases remains unknown. Neutrophils are the primary leukocytes involved in the early phase of innate immunity and inflammation. As part of their anti-microbial defense, neutrophils form extracellular traps (NETs) by releasing decondensed chromatin lined with cytotoxic proteins. NETs have been shown to induce tissue injury and thrombosis. Here, we demonstrated that Sirt3, a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase, an enzyme linked to human longevity, was expressed in mouse neutrophils and platelets...
2017: PloS One
https://www.readbyqxmd.com/read/29234042/cfdna-correlates-with-endothelial-damage-after-cardiac-surgery-with-prolonged-cardiopulmonary-bypass-and-amplifies-netosis-in-an-intracellular-tlr9-independent-manner
#7
Adnana Paunel-Görgülü, Max Wacker, Mouhamed El Aita, Shoreshfan Hassan, Georg Schlachtenberger, Antje Deppe, Yeong-Hoon Choi, Elmar Kuhn, Thorsten O Mehler, Thorsten Wahlers
Cardiopulmonary bypass (CPB) provokes inflammation culminating in organ dysfunction and increased mortality. Recently, neutrophil extracellular traps (NETs) have been found to be involved in a variety of cardiovascular diseases promoting tissue and organ injury. Here, we aimed to elaborate the proinflammatory potential of circulating cell-free (cf)DNA in patients undergoing cardiac surgery with CPB. Plasma was collected pre- and postoperatively as well as at d1, d3, d5 and d8 after surgery. At d1, we found circulating cfDNA levels to be significantly increased in patients with prolonged CPB duration (>100 min) when compared to those with shorter CPB times (CPB < 100 min)...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29203860/adipose-tissue-mitochondrial-capacity-associates-with-long-term-weight-loss-success
#8
R Jokinen, R Rinnankoski-Tuikka, S Kaye, L Saarinen, S Heinonen, M Myöhänen, E Rappou, S Jukarainen, A Rissanen, A Pessia, V Velagapudi, K A Virtanen, E Pirinen, K H Pietiläinen
OBJECTIVES: We investigated whether (1) subcutaneous adipose tissue (SAT) mitochondrial capacity predicts weight loss success and (2) weight loss ameliorates obesity-related SAT mitochondrial abnormalities. METHODS: SAT biopsies were obtained from 19 clinically healthy obese subjects (BMI 34.6±2.7 kg/m2) during a weight-loss intervention (0, 5, and 12 months) and from 19 lean reference subjects (BMI 22.7±1.1 kg/m2) at baseline. Based on one-year weight loss outcome, the subjects were divided into two groups: continuous weight losers (WL, n=6) and weight regainers (WR, n=13)...
December 5, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29197291/induced-sperm-oxidative-stress-in-dogs-susceptibility-against-different-reactive-oxygen-species-and-protective-role-of-seminal-plasma
#9
Nívea de Mattos Góes Vieira, João Diego de Agostini Losano, Daniel de Souza Ramos Angrimani, Giulia Kiyomi Vechiato Kawai, Luana de Cássia Bicudo, Bruno Rogério Rui, Bárbara do Carmo Simões da Silva, Mayra Elena Ortiz D'Avila Assumpção, Marcilio Nichi
Oxidative stress (OS) is characterized by an unbalance between increased levels of reactive oxygen species (ROS) and/or impaired antioxidant protection. In this context, the composition of seminal plasma (SP) plays a key role in protecting sperm against OS. However, reproductive biotechnologies applied to dogs recommend the removal of SP. Thus, antioxidant therapy may be an important alternative when applying biotechniques such as semen cryopreservation in this specie. However, in order to be efficient, the choice of the ideal antioxidant in each condition is essential since each ROS is preferably neutralized by different antioxidant systems...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29174262/perioperative-plasma-mitochondrial-dna-dynamics-and-correlation-with-inflammation-during-infantile-cardiopulmonary-bypass
#10
Fei Xu, Rui-Qi Liu, Rong Cao, Lang-Tao Guo, Ning Zhang, Ke Huang, Yu Cui, Wei-Na Li, Lei Li, Zheng-Hua Huang
OBJECTIVE: Numerous studies in animals and humans have demonstrated that inflammatory mediators such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 play a role in cardiopulmonary bypass (CPB), which might affect surgical outcomes. Plasma mitochondrial DNA (mtDNA), a recently discovered pro-inflammatory agent, is released by cells upon insult. This study aimed to detect changes in plasma mtDNA levels at different time points after infantile CPB and explore its potential association with inflammatory mediators...
November 2017: Indian Heart Journal
https://www.readbyqxmd.com/read/29165056/citrinin-induces-apoptosis-in-human-hct116-colon-cancer-cells-through-endoplasmic-reticulum-stress
#11
Amal Salah, Chayma Bouaziz, Alexandre Prola, Julie Pires Da Silva, Hassen Bacha, Salwa Abid-Essefi, Christophe Lemaire
The mycotoxin citrinin (CTN) is a natural contaminant of various human foods that may produce serious adverse health problems. Several studies demonstrated that citrinin exerts cytotoxic and genotoxic effects in both in vivo and in vitro systems. However, the precise mechanisms of action (MOA), particularly in intestinal cells remain unclear. The aim of the present study was to examine the precise MOA of citrinin in vitro. Data demonstrated that CTN significantly decreased the number of viable human intestinal HCT116 cells and induced apoptotic events including (1) decrease in ΔѰm indicative of mitochondrial membrane permeabilization, (2) activation of caspase 3, (3) elevated production of reactive oxygen species (ROS) and (4) relative persistence of plasma membrane integrity...
2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29164653/gradient-sperm-selection-for-reproductive-techniques-in-cattle-is-isolate-a-suitable-replacement-for-percoll
#12
B Sepúlveda, M E Arias, L Aguila, F Zambrano, R Sánchez, R Felmer
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate(®) was a suitable substitute for Percoll(®) for assisted reproductive techniques. Commercial cryopreserved bovine semen was used after selection in both gradients, and plasma and acrosome membrane integrity, reactive oxygen species (ROS) levels, DNA integrity and mitochondrial membrane potential (ΔΨm) were assessed by flow cytometry...
November 21, 2017: Andrologia
https://www.readbyqxmd.com/read/29155101/type-i-ifn-related-netosis-in-ataxia-telangiectasia-and-artemis-deficiency
#13
Ersin Gul, Esra Hazar Sayar, Bilgi Gungor, Fehime Kara Eroglu, Naz Surucu, Sevgi Keles, Sukru Nail Guner, Sıddıka Fındık, Esin Alpdündar, Ihsan Cihan Ayanoglu, Basak Kayaoglu, Busra Nur Geçkin, Hatice Asena Sanli, Tamer Kahraman, Cengiz Yakicier, Meltem Muftuoglu, Berna Oguz, Deniz Nazire Cagdas Ayvaz, Ihsan Gursel, Seza Ozen, Ismail Reisli, Mayda Gursel
BACKGROUND: Pathological inflammatory syndromes of unknown etiology are commonly observed in Ataxia telangiectasia (AT) and Artemis deficiency. Similar inflammatory manifestations also exist in STING-associated vasculopathy in infancy (SAVI) patients. OBJECTIVE: To test the hypothesis that the inflammation associated manifestations observed in AT and Artemis deficient patients stem from increased type I IFN signature leading to neutrophil mediated pathological damage...
November 15, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29147981/low-atrazine-dosages-reduce-sperm-quality-of-calomys-laucha-mice
#14
Graciela Quintana Saalfeld, Antônio Sergio Varela Junior, Tiane Castro, Fernanda Alves Pereira, Stela Mari Meneghello Gheller, Alessandra Cardoso da Silva, Carine Dahl Corcini, Carlos Eduardo da Rosa, Elton Pinto Colares
Reproductive effects caused by the exposure to environmentally relevant dosages of atrazine on wild animals are poorly understood. This study evaluated the effects of three dosages of atrazine on sperm parameters of adult Calomys laucha males. Adult mice were orally exposed to dosages of 0 (water and vehicle control), 0.1, 1, and 10 mg/kg of animal weight for a 21-day period. Following exposure, analyses were performed to determine sperm motility parameters, plasma membrane integrity and fluidity, mitochondrial functionality, acrosome integrity, DNA damage, lipid peroxidation, and production of reactive oxygen species (ROS) in the sperm samples...
November 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29130947/application-of-digital-pcr-in-detecting-human-diseases-associated-gene-mutation
#15
Yu Tong, Shizhen Shen, Hui Jiang, Zhi Chen
Gene mutation has been considered a research hotspot, and the rapid development of biomedicine has enabled significant advances in the evaluation of gene mutations. The advent of digital polymerase chain reaction (dPCR) elevates the detection of gene mutations to unprecedented levels of precision, especially in cancer-associated genes. dPCR has been utilized in the detection of tumor markers in cell-free DNA (cfDNA) samples from patients with different types of cancer in samples such as plasma, cerebrospinal fluid, urine and sputum, which confers significant value for dPCR in both clinical applications and basic research...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29113834/aflatoxin-b1-impairs-sperm-quality-and-fertilization-competence
#16
A Komsky-Elbaz, M Saktsier, Z Roth
Aflatoxins are poisonous byproducts of the soilborne fungus Aspergillus, involved in the decomposition of plant materials. Aflatoxins can be found in various food products, such as maize, sorghum, millet, rice and wheat. AFB1 is the most toxic of these, classified as a carcinogen and mutagen for both humans and animals. AFB1 has been detected in human cord blood and placenta; however, its toxic effect on sperm is less known. The current study examines sperm responses associated with AFB1 exposure. These included acrosome integrity and function, mitochondrial polarity, DNA fragmentation, fertilization competence and early embryonic development...
January 15, 2018: Toxicology
https://www.readbyqxmd.com/read/29110394/methylisothiazolinone-may-induce-cell-death-and-inflammatory-response-through-dna-damage-in-human-liver-epithelium-cells
#17
Eun-Jung Park, Sanghwa Kim, Jaerak Chang
Methylisothiazolinone (MIT) is a powerful biocide and preservative, which is widely used alone or in a 1:3 ratio with methylchloroisothiazolinone (MCIT) under the trade name of Kathons in the manufacture of numerous personal and household products. Considering that Kathons injected intravenously is distributed in the blood and then in the liver, we explored the toxic mechanism of MIT on human liver epithelium cells. At 24 h after exposure, MIT bound to the plasma membrane and the inner wall of vacuoles in the cells, and rupture of the cell membrane and nuclear envelop, autophagosome-like vacuoles formation and mitochondrial damage were observed...
November 7, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/29104026/current-insights-into-functions-of-phospholipase-a2-receptor-in-normal-and-cancer-cells-more-questions-than-answers
#18
REVIEW
Olga Sukocheva, Mario Menschikowski, Albert Hagelgans, Nagendra Sastry Yarla, Gabriele Siegert, Pallu Reddanna, Anupam Bishayee
Lipid signaling network was proposed as a potential target for cancer prevention and treatment. Several recent studies revealed that phospholipid metabolising enzyme, phospholipase A2 (PLA2), is a critical regulator of cancer accelerating pathologies and apoptosis in several types of cancers. In addition to functioning as an enzyme, PLA2 can activate a phospholipase A2 receptor (PLA2R1) in plasma membrane. While the list of PLA2 targets extends to glucose homeostasis, intracellular energy balance, adipocyte development, and hepatic lipogenesis, the PLA2R1 downstream effectors are few and scarcely investigated...
November 2, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/29078758/transcriptomic-data-from-two-primary-cell-models-stimulating-human-monocytes-suggest-inhibition-of-oxidative-phosphorylation-and-mitochondrial-function-by-n-meningitidis-which-is-partially-up-regulated-by-il-10
#19
Unni Gopinathan, Reidun Øvstebø, Berit Sletbakk Brusletto, Ole Kristoffer Olstad, Peter Kierulf, Petter Brandtzaeg, Jens Petter Berg
BACKGROUND: Biological interpretation of DNA microarray data may differ depending on underlying assumptions and statistical tests of bioinformatics tools used. We used Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) to analyze previously generated DNA microarray data from human monocytes stimulated with N. meningitidis and IL-10 ("the model system"), and with meningococcal sepsis plasma before and after immunodepletion of IL-10 ("the patient plasma system"). The objectives were to compare if the two bioinformatics methods resulted in similar biological interpretation of the datasets, and to identify whether GSEA provided additional insight compared with IPA about the monocyte host response to meningococcal activation...
October 27, 2017: BMC Immunology
https://www.readbyqxmd.com/read/29074536/a-novel-application-of-structural-equation-modeling-estimates-the-association-between-oxidative-stress-and-colorectal-adenoma
#20
Ronald C Eldridge, Michael Goodman, Roberd M Bostick, Veronika Fedirko, Myron Gross, Bharat Thyagarajan, W Dana Flanders
In vitro evidence implicates oxidative stress in many adverse health conditions, including colorectal neoplasia. In human studies, however, oxidative stress is measured by imperfect biomarkers, which are inconsistently associated with health outcomes. Structural equation modeling (SEM) offers one possible solution by modeling a latent (unobserved) construct from multiple biomarkers. Our goal was to investigate the association of a latent oxidative stress variable with colorectal adenoma. Using SEM, we analyzed pooled data from two cross-sectional studies of colorectal adenoma (n=526) that measured five plasma biomarkers of oxidative stress and inflammation that comprised the latent oxidative stress variable: F2-isoprostanes (FIP), fluorescent oxidation products (FOP), mitochondrial DNA (MtDNA) copy number, γ-tocopherol (Gtoc), and C-reactive protein (CRP)...
October 26, 2017: Cancer Prevention Research
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