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Plasma mitochondrial dna

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https://www.readbyqxmd.com/read/28073488/plasma-mitochondrial-dna-levels-were-independently-associated-with-lung-injury-in-elderly-hip-fracture-patients
#1
Jian-Zheng Zhang, Juan Wang, Wen-Chun Qu, Xiao-Wei Wang, Zhi Liu, Ji-Xin Ren, Li Han, Tian-Sheng Sun
INTRODUCTION: Hip fracture in the elderly can induce systemic inflammatory response (SIRS) and lung injury which increases the risk of lung infection and death. Mitochondrial DNA (mtDNA) plays a role in SIRS and lung injury in patients with multi-trauma, and also in patients with hip fractures. This study evaluated the potential value of plasma mtDNA in the early prognosis of lung injury in elderly fracture patients. METHODS: This study enrolled 156 elderly patients with intertrochanteric fracture...
January 4, 2017: Injury
https://www.readbyqxmd.com/read/28070494/pure-myopathy-with-enlarged-mitochondria-associated-to-a-new-mutation-in-mtnd2-gene
#2
Alice Zanolini, Ana Potic, Franco Carrara, Eleonora Lamantea, Daria Diodato, Flavia Blasevich, Silvia Marchet, Marina Mora, Francesco Pallotti, Lucia Morandi, Massimo Zeviani, Costanza Lamperti
To date, only few mutations in the mitochondrial DNA (mtDNA)-encoded ND2 subunit of Complex I have been reported, usually presenting a severe phenotype characterized by early onset encephalomyopathy and early death. In this report, we describe a new mutation in the MTND2 gene in a 21-year-old man with a mild myopathic phenotype characterized by exercise intolerance and increased plasma lactate at rest. Electromyography and brain NMR were normal, and no cardiac involvement was present. Muscle biopsy showed a massive presence of ragged red - COX-positive fibres, with enlarged mitochondria containing osmiophilic inclusions...
March 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28053624/alkyl-cinnamates-induce-protein-kinase-c-translocation-and-anticancer-activity-against-breast-cancer-cells-through-induction-of-the-mitochondrial-pathway-of-apoptosis
#3
Suman Jyoti Deka, Narsimha Mamdi, Debasis Manna, Vishal Trivedi
PURPOSE: The protein kinase C (PKC) family of serine-threonine kinases plays an important role in cancer cell progression. Thus, molecules that target PKC have potential as anticancer agents. The current study aims to understand the treatment of breast cancer cells with alkyl cinnamates. We have also explored the mechanistic details of their anticancer action and the underlying molecular signaling. METHODS: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to measure the viability of MDAMB-231 breast cancer cells to assess the anticancer activity of these compounds...
December 2016: Journal of Breast Cancer
https://www.readbyqxmd.com/read/28049543/the-level-of-free-circulating-mitochondrial-dna-in-blood-as-predictor-of-death-in-case-of-acute-coronary-syndrome
#4
Nikolay P Sudakov, Konstantin A Apartsin, Svetlana A Lepekhova, Sergey B Nikiforov, Alexander I Katyshev, Galina I Lifshits, Anna V Vybivantseva, Yuri M Konstantinov
BACKGROUND: The efficacy of treating acute myocardial ischemic damages depends, to a large extent, on the development of technologies for predicting their course and outcome. The aim of this paper was to explore whether it would be possible to consider the content of free circulating mitochondrial DNA as a danger-associated molecular pattern for assessing the probability of death from myocardial infarction. METHODS: We have analyzed the clinical outcomes based on discharge summaries and autopsy reports obtained in the course of the PROTOCOL observational trial...
January 3, 2017: European Journal of Medical Research
https://www.readbyqxmd.com/read/28043368/chicken-egg-yolk-plasma-in-tris-citric-acid-extender-improves-the-quality-and-fertility-of-cryopreserved-water-buffalo-bubalus-bubalis-spermatozoa
#5
S Aftab Hussain Shah, S Murtaza Hassan Andrabi, Hussain Ahmed, Irfan Zia Qureshi
This study was primarily designed to evaluate the effect of different concentrations of ultraviolet (UV)-C-irradiated chicken egg yolk plasma (EYP; v:v; 10%, P1; 15%, P2; 20%, P3) or 20% (v:v) of whole chicken egg yolk (WCEY) in tris-citric acid (TCA) extender on water buffalo sperm quality during cryopreservation (postdilution, PD; postequilibration, PE; post-thawing, PT). Also the effect of best evolved concentration of UV-C-irradiated EYP in extender on in vivo fertility of buffalo spermatozoa was evaluated...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043360/effects-of-an-extension-of-the-equilibration-period-up-to-96%C3%A2-hours-on-the-characteristics-of-cryopreserved-bull-semen
#6
A Fleisch, E Malama, U Witschi, C Leiding, M Siuda, F Janett, H Bollwein
This study was designed to investigate the effects of an equilibration period up to 96 hours and three extenders (AndroMed, OPTIXcell, and Triladyl) on the quality of cryopreserved bull semen and to evaluate, whether an extension of the equilibration time to 72 hours does affect fertility in the field. One ejaculate of 17 bulls was collected and divided into three equal aliquots and diluted, respectively, with the three extenders. Each aliquot was again divided into five parts and equilibrated for 4, 24, 48, 72, and 96 hours before freezing in an automatic freezer...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043345/effect-of-different-semen-extenders-for-the-storage-of-chilled-sperm-in-tigrina-leopardus-tigrinus
#7
D S R Angrimani, P M H Barros, J D A Losano, C N M Cortada, R P Bertolla, M A B V Guimarães, S H R Correa, V H Barnabe, M Nichi
To enhance the conservation of endangered populations, the present study aimed to evaluate whether Tigrinas (Leopardus tigrinus) sperm could be conserved under refrigeration for short periods while maintaining sufficient quality for use in assisted-reproductive techniques (i.e., cryopreservation, in vitro fertilization). For this purpose, semen samples from 15 Tigrinas individuals were submitted to conventional and functional tests after different cooling periods (4 °C; 0, 12, and 24 hours postcooling), using TCM 199 (TCM), Ham's F10 (HAM), Ham's F10 with bovine serum albumin (HBSA), and Tris-citrate egg yolk (TEYC) extenders...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28041717/the-effect-of-cholesterol-loaded-cyclodextrins-on-post-thawing-quality-of-buffalo-semen-in-relation-to-sperm-dna-damage-and-ultrastructure
#8
M F Abouelezz, A E Montaser, M S Hussein, A M Eldesouky, M R Badr, A O Hegab, A Z Balboula, S M Zaabel
The cryopreservation of germ cells is a major tool for the propagation of animals with desired genetic traits. Although cryopreservation of spermatozoa in some animals is effective, its effectiveness is variable. For example, cryopreservation efficiency of buffalo bull spermatozoa remains very poor. In this study, we evaluated sperm DNA damage and ultrastructure in buffalo bull spermatozoa vitrified in the presence or absence of cholesterol-loaded cyclodextrins (CLC). Our results showed that cryopreserved buffalo spermatozoa had elevated levels of deteriorated plasma and mitochondrial membranes, which are the likely causes of DNA damage after vitrification...
December 29, 2016: Reproductive Biology
https://www.readbyqxmd.com/read/28004624/intrauterine-growth-restriction-increases-circulating-mitochondrial-dna-and-toll-like-receptor-9-expression-in-adult-offspring-could-aerobic-training-counteract-these-adaptations
#9
V Oliveira, S D Silva Junior, M H C de Carvalho, E H Akamine, L C Michelini, M C Franco
It has been demonstrated that intrauterine growth restriction (IUGR) can program increase cardiometabolic risk. There are also evidences of the correlation between IUGR with low-grade inflammation and, thus can contribute to development of several cardiometabolic comorbidities. Therefore, we investigated the influence of IUGR on circulating mitochondrial DNA (mtDNA)/Toll-like receptor 9 (TLR9) and TNF-α expression in adult offspring. Considering that the aerobic training has anti-inflammatory actions, we also investigated whether aerobic training would improve these inflammatory factors...
December 22, 2016: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28002613/different-responses-of-vanillic-acid-a-phenolic-compound-in-htc-cells-cytotoxicity-antiproliferative-activity-and-protection-from-dna-induced-damage
#10
I V Almeida, F M L Cavalcante, V E P Vicentini
The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used widely in the food industry as a flavoring, preservative, and food additive. VA can be found in various cereals, whole grains, fruits, herbs, green tea, juices, beers, and wines and possesses antioxidant, hepatoprotective, cardioprotective, and antiapoptotic activities...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27933735/danazol-has-potential-to-cause-pkc-translocation-cell-cycle-dysregulation-and-apoptosis-in-breast-cancer-cells
#11
Suman Jyoti Deka, Ashalata Roy, Vibin Ramakrishnan, Debasis Manna, Vishal Trivedi
Danazol, the established clinical drug has given promising therapeutic results in a series of clinical trials with breast cancer patients. Danazol shares structural similarities with several known PKC agonists and fits well into the C1 domain. Danazol binds to the C1b domain of PKC with Kd of 5.64±1.27 μM. MD simulation studies further support that the PKC-Danazol molecular model is stable and showing minimum distortion to the structure during the simulation period. Immunofluorescence and western-blotting studies indicate that MDAMB-231 cells stimulated with danazol exhibit translocation of PKCα to the plasma membrane...
December 9, 2016: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/27927977/ppar-mrna-levels-are-modified-by-dietary-n-3-fatty-acid-restriction-and-energy-restriction-in-the-brain-and-liver-of-growing-rats
#12
Matthew J Picklo, LuAnn Johnson, Joseph Idso
BACKGROUND: Without dietary sources of n-3 (ω-3) long-chain polyunsaturated fatty acids (LCPUFAs), α-linolenic acid (ALA; 18:3n-3) is the precursor for docosahexaenoic acid (DHA; 22:6n-3). It is not known how energy restriction (ER) affects ALA conversion to DHA. OBJECTIVE: We tested the hypothesis that ER reduces n-3 LCPUFA concentrations in tissues of growing rats fed diets replete with and deficient in ALA. METHODS: Male Sprague-Dawley rats (23 d old) were provided AIN93G diets (4 wk) made with soybean oil (SO; ALA sufficient) or corn oil (CO; ALA deficient) providing 16% of energy as fat...
December 7, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27922635/increased-plasma-levels-of-circulating-cell-free-mitochondrial-dna-in-suicide-attempters-associations-with-hpa-axis-hyperactivity
#13
D Lindqvist, J Fernström, C Grudet, L Ljunggren, L Träskman-Bendz, L Ohlsson, Å Westrin
Preclinical data suggest that chronic stress may cause cellular damage and mitochondrial dysfunction, potentially leading to the release of mitochondrial DNA (mtDNA) into the bloodstream. Major depressive disorder has been associated with an increased amount of mtDNA in leukocytes from saliva samples and blood; however, no previous studies have measured plasma levels of free-circulating mtDNA in a clinical psychiatric sample. In this study, free circulating mtDNA was quantified in plasma samples from 37 suicide attempters, who had undergone a dexamethasone suppression test (DST), and 37 healthy controls...
December 6, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27921220/increased-cell-free-mitochondrial-dna-is-a-marker-of-ongoing-inflammation-and-better-neurocognitive-function-in-virologically-suppressed-hiv-infected-individuals
#14
Josué Pérez-Santiago, Michelli F De Oliveira, Susanna R Var, Tyler R C Day, Steven P Woods, Sara Gianella, Sanjay R Mehta
Cell-free mitochondrial DNA (mtDNA) is a highly immunogenic molecule that is associated with several inflammatory conditions and with neurocognitive impairment during untreated HIV infection. Here, we investigate how cell-free mtDNA in cerebrospinal fluid (CSF) is associated with inflammation, neuronal damage, and neurocognitive functioning in the context of long-term suppressive antiretroviral therapy (ART). We quantified the levels of cell-free mtDNA in the CSF from 41 HIV-infected individuals with completely suppressed HIV RNA levels in blood plasma (<50 copies/mL) by droplet digital PCR...
December 5, 2016: Journal of Neurovirology
https://www.readbyqxmd.com/read/27917263/changes-in-cellular-proliferation-and-plasma-products-are-associated-with-liver-failure
#15
Juliana Gil Melgaço, Frederico Marianetti Soriani, Pedro Henrique Ferreira Sucupira, Leonardo Assaf Pinheiro, Yasmine Rangel Vieira, Jaqueline Mendes de Oliveira, Lia Laura Lewis-Ximenez, Cristina Carvalho Vianna Araújo, Lúcio Filgueiras Pacheco-Moreira, Gustavo Batista Menezes, Oswaldo Gonçalves Cruz, Claudia Lamarca Vitral, Marcelo Alves Pinto
AIM: To study the differences in immune response and cytokine profile between acute liver failure and self-limited acute hepatitis. METHODS: Forty-six patients with self-limited acute hepatitis (AH), sixteen patients with acute liver failure (ALF), and twenty-two healthy subjects were involved in this study. The inflammatory and anti-inflammatory products in plasma samples were quantified using commercial enzyme-linked immunoassays and quantitative real-time PCR...
November 18, 2016: World Journal of Hepatology
https://www.readbyqxmd.com/read/27821553/potential-biomarkers-to-follow-the-progression-and-treatment-response-of-huntington-s-disease
#16
Marie-Hélène Disatnik, Amit U Joshi, Nay L Saw, Mehrdad Shamloo, Blair R Leavitt, Xin Qi, Daria Mochly-Rosen
Huntington's disease (HD) is a rare genetic disease caused by expanded polyglutamine repeats in the huntingtin protein resulting in selective neuronal loss. Although genetic testing readily identifies those who will be affected, current pharmacological treatments do not prevent or slow down disease progression. A major challenge is the slow clinical progression and the inability to biopsy the affected tissue, the brain, making it difficult to design short and effective proof of concept clinical trials to assess treatment benefit...
November 14, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27814627/oxidative-dna-and-mitochondrial-dna-change-in-patients-with-sle
#17
Hui-Ting Lee, Tsai-Hung Wu, Chen-Sung Lin, Chyou-Shen Lee, Siao-Cian Pan, Deh-Ming Chang, Yau-Huei Wei, Chang-Youh Tsai
We evaluated plasma IL-10, IFN-alpha, IL-23, IFN-gamma, IP-10, MCP-1, 8-OHdG, leukocyte mtDNA, serum anti-dsDNA antibodies and disease activity index (SLEDAI) in SLE patients. 93 patients (35 nephritis, 4 under dialysis, 5 under rituximab) and 50 healthy controls were recruited. Compared with healthy controls, SLE patients had higher IL-10, IFN-alpha, IL-23, IFN-gamma, IP-10 and MCP-1 (p<0.05). High IFN-alpha (p=0.031) and IP-10 (p=0.026) correlated with high SLEDAI; high IFN-alpha (p<0.001), IL-23 (p=0...
January 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/27811985/elevated-homocysteine-levels-in-type-2-diabetes-induce-constitutive-neutrophil-extracellular-traps
#18
Manjunath B Joshi, Guruprasad Baipadithaya, Aswath Balakrishnan, Mangala Hegde, Manik Vohra, Rayees Ahamed, Shivashankara K Nagri, Lingadakai Ramachandra, Kapaettu Satyamoorthy
Constitutively active neutrophil extracellular traps (NETs) and elevated plasma homocysteine are independent risk factors for Type 2 Diabetes (T2D) associated vascular diseases. Here, we show robust NETosis due to elevated plasma homocysteine levels in T2D subjects and increased components of NETs such as neutrophil elastase and cell free DNA. Cooperative NETs formation was observed in neutrophils exposed to homocysteine, IL-6 and high glucose suggesting acute temporal changes tightly regulate constitutive NETosis...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27811968/very-short-mitochondrial-dna-fragments-and-heteroplasmy-in-human-plasma
#19
Ruoyu Zhang, Kiichi Nakahira, Xiaoxian Guo, Augustine M K Choi, Zhenglong Gu
Cell free DNA (cfDNA) has received increasing attention and has been studied in a broad range of clinical conditions. However, few studies have focused on mitochondrial DNA (mtDNA) in the cell free form. We optimized DNA isolation and sequencing library preparation protocols to better retain short DNA fragments from plasma, and applied these optimized methods to plasma samples from patients with sepsis. Our methods can retain substantially shorter DNA, resulting in an average of 11.5 fold increase in short DNA fragments yield (DNA <100bp)...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27773698/differential-susceptibility-to-acetaminophen-induced-liver-injury-in%C3%A2-sub-strains-of-c57bl-6-mice-6n-versus-6j
#20
Luqi Duan, John S Davis, Benjamin L Woolbright, Kuo Du, Mala Cahkraborty, James Weemhoff, Hartmut Jaeschke, Mohammed Bourdi
: Mouse models of acetaminophen (APAP) hepatotoxicity are considered relevant for the human pathophysiology. The C57BL/6 strain is most popular because it is the background strain of gene knock-out mice. However, conflicting results in the literature may have been caused by sub-strain mismatches, e.g. C57BL/6J and C57BL/6N. This study was initiated to determine the mechanism behind the sub-strain susceptibility to APAP toxicity. C57BL/6N and C57BL/6J mice were dosed with 200 mg/kg APAP and sacrificed at different time points...
December 2016: Food and Chemical Toxicology
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