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Plasma mitochondrial dna

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https://www.readbyqxmd.com/read/28228506/low-plasma-zinc-is-associated-with-higher-mitochondrial-oxidative-stress-and-faster-liver-fibrosis-development-in-the-miami-adult-studies-in-hiv-cohort
#1
Sabrina S Martinez, Adriana Campa, Yinghui Li, Christina Fleetwood, Tiffanie Stewart, Venkataraghavan Ramamoorthy, Marianna K Baum
Background: Oxidative stress and reduced antioxidants may be a trigger for liver fibrogenesis. Reducing oxidative stress through higher antioxidant concentration may be a potential antifibrotic target.Objective: We aimed to investigate longitudinally whether plasma zinc, an antioxidant, is related to mitochondrial oxidative stress and the progression of liver fibrosis in the Miami Adult Studies in HIV (MASH) cohort.Methods: A prospective observational cohort study was conducted in 487 predominantly African American HIV-monoinfected and HIV/hepatitis C virus (HCV)-coinfected adults with a mean ± SD age of 47...
February 22, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28220120/neutrophil-extracellular-traps-and-its-implications-in-inflammation-an-overview
#2
REVIEW
Vidal Delgado-Rizo, Marco A Martínez-Guzmán, Liliana Iñiguez-Gutierrez, Alejandra García-Orozco, Anabell Alvarado-Navarro, Mary Fafutis-Morris
In addition to physical barriers, neutrophils are considered a part of the first line of immune defense. They can be found in the bloodstream, with a lifespan of 6-8 h, and in tissue, where they can last up to 7 days. The mechanisms that neutrophils utilize for host defense are phagocytosis, degranulation, cytokine production, and, the most recently described, neutrophil extracellular trap (NET) production. NETs are DNA structures released due to chromatin decondensation and spreading, and they thus occupy three to five times the volume of condensed chromatin...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28209603/deoxyribonuclease-partially-ameliorates-thioacetamide-induced-hepatorenal-injury
#3
Lenka Vokálová, Lucia Lauková, Jozef Čonka, Veronika Melišková, Veronika Borbélyová, Janka Bábíčková, Ľubomíra Tóthová, Július Hodosy, Barbora Vlková, Peter Celec
Several recent studies have shown that liver injury is associated with the release of DNA from hepatocytes. This DNA stimulates innate immunity and induces sterile inflammation exacerbating the liver damage. Similar mechanisms have been described for acute renal injury. Deoxyribonuclease degrades cell-free DNA and can potentially prevent some of the induced tissue damage. In this study the effects of thioacetamide-induced hepatorenal injury on plasma DNA in rats were analysed. Plasma DNA of both nuclear and mitochondrial origin was higher in thioacetamide-treated animals...
February 16, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28205391/influence-of-iodine-in-excess-on-seminiferous-tubular-structure-and-epididymal-sperm-character-in-male-rats
#4
Amar K Chandra, Arijit Chakraborty
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress in the testis. However, information on the possible effect of iodine in excess on spermatozoa found less. In the present investigation flow cytometric techniques and scanning electron microscopy (SEM) have been used to study the spermatozoal functional as well as structural status under the influence of excess iodine; generation of ROS in the spermatozoa as evident by DCFDA, altered acrosomal integrity as observed by fluorescence lectin staining method and depolarized mitochondrial membrane potential (ΔΨm ) noticed by JC-1 staining...
February 16, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28198717/mitochondrial-dna-induced-inflammatory-responses-and-lung-injury-in-thermal-injury-rat-model-protective-effect-of-epigallocatechin-gallate
#5
Ruiqi Liu, Fei Xu, Si Si, Xueshan Zhao, Siwei Bi, Ying Cen
Lungs are easily damaged by the inflammatory responses induced after extensive burns. The aim here was to investigate the protective role of epigallocatechin gallate (EGCG) in mitochondrial DNA (mtDNA)-mediated inflammatory responses and acute respiratory distress syndrome (ARDS) in a rat model of thermal injury. Male Sprague-Dawley rats were randomly assigned to five groups. In the first experiment, a full-thickness thermal injury or control procedure, covering 30% of the TBSA, was inflicted on three groups designated as the thermal injury, EGCG, and sham control groups...
February 6, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28190385/evidence-of-pkc-binding-and-translocation-to-explain-the-anticancer-mechanism-of-chlorogenic-acid-in-breast-cancer-cells
#6
S J Deka, S Gorai, D Manna, V Trivedi
Chlorogenic acid (CGA) exhibits potentials towards liver, breast and skin cancer. Cancer cells stimulated with CGA exhibits differential expression of transcriptional factors and regulatory molecules but the molecular target of the molecule is not known. Superposition of biophoric elements of CGA with Curcumin gives maximum common substructure score of 0.90. Molecular modeling studies further suggest that CGA fits into the C1b domain of PKC with extensive interaction with residues lining binding site. It binds PKC in a concentration dependent manner with dissociation constant KD, 28...
February 9, 2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/28187743/the-influence-of-macro-and-microelements-in-seminal-plasma-on-diluted-boar-sperm-quality
#7
Maja Zakošek Pipan, Janko Mrkun, Breda Jakovac Strajn, Katarina Pavšič Vrtač, Janko Kos, Anja Pišlar, Petra Zrimšek
BACKGROUND: Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15-17 °C...
February 10, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28174514/associations-among-cognitive-functions-plasma-dna-and-white-matter-integrity-in-patients-with-early-onset-parkinson-s-disease
#8
Yueh-Sheng Chen, Meng-Hsiang Chen, Cheng-Hsien Lu, Pei-Chin Chen, Hsiu-Ling Chen, I-Hsiao Yang, Nai-Wen Tsai, Wei-Che Lin
Early-onset Parkinson's disease (EOPD) patients are symptomatic at a relatively young age, and the impacts of the disease on both the patients and their caregivers are dramatic. Few studies have reported on the cognitive impairments seen in EOPD, and the results of these studies have been diverse. Furthermore, it is still unclear what microstructural white matter (WM) changes are present in EOPD patients. As such, we conducted this study to investigate the microstructural WM changes experienced by EOPD patients and their association with cognitive function and plasma DNA levels...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28166956/impact-of-induced-levels-of-specific-free-radicals-and-malondialdehyde-on-chicken-semen-quality-and-fertility
#9
Bruno R Rui, Fábio Y Shibuya, Allison J T Kawaoku, João D A Losano, Daniel S R Angrimani, Andressa Dalmazzo, Marcilio Nichi, Ricardo J G Pereira
Over the past decades, scientists endeavored to comprehend oxidative stress in poultry spermatozoa and its relationship with fertilizing ability, lipid peroxidation (LPO), free-radical scavenging systems, and antioxidant therapy. Although considerable progress has been made, further improvement is needed in understanding how specific reactive oxygen species (ROS) and malondialdehyde (MDA, a toxic byproduct of LPO) disrupt organelles in avian spermatozoon. Hence, this study examined functional changes in chicken spermatozoa after incubation with different ROS, and their implications for the fertility...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28163707/autophagy-is-impaired-in-neutrophils-from-streptozotocin-induced-diabetic-rats
#10
Wilson Mitsuo Tatagiba Kuwabara, Rui Curi, Tatiana Carolina Alba-Loureiro
We tested the hypothesis that changes reported on functions of neutrophils from streptozotocin-induced diabetic rats involve autophagy impairment. Wistar rats were rendered diabetic by streptozotocin injection (65 mg/kg, i.v.), and the measurements were carried out 2 weeks afterward. Neutrophils were collected through intraperitoneal cavity lavage after 4 h of i.p. oyster glycogen type 2 injection. Neutrophils cultured with PMA (20 nM) for 1 h were used for analysis of plasma membrane integrity, DNA fragmentation, and mitochondrial depolarization by flow cytometry; expression of Atg5, Atg14, Beclin1, LC3BII, and Rab9 by RT-PCR; the contents of caspase 3, LC3BII/LC3BI, and pS6 by western blotting; ATP content by fluorescence essay; reactive oxygen species production by chemiluminescence (Luminol), and autophagy by immunofluorescence tracking LC3B cleavage...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28154880/accurate-quantitation-of-circulating-cell-free-mitochondrial-dna-in-plasma-by-droplet-digital-pcr
#11
Wei Ye, Xiaojun Tang, Chu Liu, Chaowei Wen, Wei Li, Jianxin Lyu
To establish a method for accurate quantitation of circulating cell-free mitochondrial DNA (ccf-mtDNA) in plasma by droplet digital PCR (ddPCR), we designed a ddPCR method to determine the copy number of ccf-mtDNA by amplifying mitochondrial ND1 (MT-ND1). To evaluate the sensitivity and specificity of the method, a recombinant pMD18-T plasmid containing MT-ND1 sequences and mtDNA-deleted (ρ(0)) HeLa cells were used, respectively. Subsequently, different plasma samples were prepared for ddPCR to evaluate the feasibility of detecting plasma ccf-mtDNA...
February 2, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28135329/hepatic-effects-of-pharmacological-doses-of-hydroxy-cobalamin-c-lactam-in-mice
#12
Patrizia Haegler, David Grünig, Benjamin Berger, Luigi Terracciano, Stephan Krähenbühl, Jamal Bouitbir
The vitamin B12 analog hydroxy-cobalamin[c-lactam] (HCCL) impairs hepatic mitochondrial protein synthesis and function of the electron transport chain in rats. We aimed to establish an in vivo model for mitochondrial dysfunction in mice, which could be used to investigate hepatotoxicity of mitochondrial toxicants. In a first step, we performed a dose-finding study in mice treated with HCCL 0.4 mg/kg and 4 mg/kg i.p. for two to four weeks. The plasma methylmalonate concentration was strongly increased at 4 mg/kg starting at three weeks of treatment...
2017: PloS One
https://www.readbyqxmd.com/read/28126408/characterization-of-mitochondrial-injury-after-cardiac-arrest-comica
#13
Michael W Donnino, Xiaowen Liu, Lars W Andersen, Jon C Rittenberger, Benjamin S Abella, David F Gaieski, Joseph P Ornato, Raúl J Gazmuri, Anne V Grossestreuer, Michael N Cocchi, Antonio Abbate, Amy Uber, John Clore, Mary Anne Peberdy, Clifton W Callaway
INTRODUCTION: Mitochondrial injury post-cardiac arrest has been described in pre-clinical settings but the extent to which this injury occurs in humans remains largely unknown. We hypothesized that increased levels of mitochondrial biomarkers would be associated with mortality and neurological morbidity in post-cardiac arrest subjects. METHODS: We performed a prospective multicenter study of post-cardiac arrest subjects. Inclusion criteria were comatose adults who suffered an out-of-hospital cardiac arrest...
January 23, 2017: Resuscitation
https://www.readbyqxmd.com/read/28115640/clonidine-induces-apoptosis-of-human-corneal-epithelial-cells-through-death-receptors-mediated-mitochondria-dependent-signaling-pathway
#14
Dan Fan, Ting-Jun Fan
Clonidine, an α2-adrenoreceptor agonist, is an anti-glaucoma drug clinically used in many developing countries, and its abuse might damage the cornea and impair human vision. However, its cytotoxicity and precise mechanisms need to be elucidated. Herein, we investigated the cytotoxicity of clonidine and its underlying mechanisms, using an in vitro model of human corneal epithelial (HCEP) cells and an in vivo model of cat corneas, respectively. HCEP cells were treated with various doses of clonidine for 1-28 h, resulting in abnormal morphology, decline of cell viability and G1 phase arrest in a time- and/or dose- dependent manner...
January 23, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28112527/identification-of-a-novel-hadhb-gene-mutation-in-an-iranian-patient-with-mitochondrial-trifunctional-protein-deficiency
#15
Mahdiyeh Shahrokhi, Mohammad Shafiei, Hamid Galehdari, Gholamreza Shariati
INTRODUCTION: Mitochondrial trifunctional protein (MTP) is a hetero-octamer composed of eight parts (subunits): four α-subunits containing LCEH (long-chain 2,3-enoyl-CoA  hydratase) and LCHAD (long-chain 3-hydroxyacyl CoA dehydrogenase) activity, and four β-subunits that possess LCKT (long-chain  3-ketoacyl-CoA thiolase) activity which catalyzes three out of four steps in β-oxidation spiral of long-chain fatty acid. Its deficiency is an autosomal recessive disorder that causes a clinical spectrum of diseases...
January 2017: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/28100767/effects-of-season-on-bull-sperm-quality-in-thawed-samples-in-northern-spain
#16
M Sabés-Alsina, A Johannisson, N Lundeheim, M Lopez-Bejar, J M Morrell
The aim of the present study was to evaluate the possible effects of climate factors on sperm quality of Holstein dairy bulls housed in northern Spain. Semen samples from 11 Holstein dairy bulls were collected and cryopreserved in winter, spring and summer. Sperm quality parameters such as motility, morphology, plasma membrane integrity, acrosome status, mitochondrial membrane potential, DNA fragmentation index and reactive oxygen species were assessed. Samples collected in spring showed higher mean values of total and progressive motility compared with samples collected in winter...
January 18, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28073488/plasma-mitochondrial-dna-levels-were-independently-associated-with-lung-injury-in-elderly-hip-fracture-patients
#17
Jian-Zheng Zhang, Juan Wang, Wen-Chun Qu, Xiao-Wei Wang, Zhi Liu, Ji-Xin Ren, Li Han, Tian-Sheng Sun
INTRODUCTION: Hip fracture in the elderly can induce systemic inflammatory response (SIRS) and lung injury which increases the risk of lung infection and death. Mitochondrial DNA (mtDNA) plays a role in SIRS and lung injury in patients with multi-trauma, and also in patients with hip fractures. This study evaluated the potential value of plasma mtDNA in the early prognosis of lung injury in elderly fracture patients. METHODS: This study enrolled 156 elderly patients with intertrochanteric fracture...
February 2017: Injury
https://www.readbyqxmd.com/read/28070494/pure-myopathy-with-enlarged-mitochondria-associated-to-a-new-mutation-in-mtnd2-gene
#18
Alice Zanolini, Ana Potic, Franco Carrara, Eleonora Lamantea, Daria Diodato, Flavia Blasevich, Silvia Marchet, Marina Mora, Francesco Pallotti, Lucia Morandi, Massimo Zeviani, Costanza Lamperti
To date, only few mutations in the mitochondrial DNA (mtDNA)-encoded ND2 subunit of Complex I have been reported, usually presenting a severe phenotype characterized by early onset encephalomyopathy and early death. In this report, we describe a new mutation in the MTND2 gene in a 21-year-old man with a mild myopathic phenotype characterized by exercise intolerance and increased plasma lactate at rest. Electromyography and brain NMR were normal, and no cardiac involvement was present. Muscle biopsy showed a massive presence of ragged red - COX-positive fibres, with enlarged mitochondria containing osmiophilic inclusions...
March 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28053624/alkyl-cinnamates-induce-protein-kinase-c-translocation-and-anticancer-activity-against-breast-cancer-cells-through-induction-of-the-mitochondrial-pathway-of-apoptosis
#19
Suman Jyoti Deka, Narsimha Mamdi, Debasis Manna, Vishal Trivedi
PURPOSE: The protein kinase C (PKC) family of serine-threonine kinases plays an important role in cancer cell progression. Thus, molecules that target PKC have potential as anticancer agents. The current study aims to understand the treatment of breast cancer cells with alkyl cinnamates. We have also explored the mechanistic details of their anticancer action and the underlying molecular signaling. METHODS: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to measure the viability of MDAMB-231 breast cancer cells to assess the anticancer activity of these compounds...
December 2016: Journal of Breast Cancer
https://www.readbyqxmd.com/read/28049543/the-level-of-free-circulating-mitochondrial-dna-in-blood-as-predictor-of-death-in-case-of-acute-coronary-syndrome
#20
MULTICENTER STUDY
Nikolay P Sudakov, Konstantin A Apartsin, Svetlana A Lepekhova, Sergey B Nikiforov, Alexander I Katyshev, Galina I Lifshits, Anna V Vybivantseva, Yuri M Konstantinov
BACKGROUND: The efficacy of treating acute myocardial ischemic damages depends, to a large extent, on the development of technologies for predicting their course and outcome. The aim of this paper was to explore whether it would be possible to consider the content of free circulating mitochondrial DNA as a danger-associated molecular pattern for assessing the probability of death from myocardial infarction. METHODS: We have analyzed the clinical outcomes based on discharge summaries and autopsy reports obtained in the course of the PROTOCOL observational trial...
January 3, 2017: European Journal of Medical Research
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