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colorectal cancer AND lncRNA

Hajar Rezanejad Bardaji, Malek Hossein Asadi, Mohammad Mehdi Yaghoobi
Emerging evidence indicates that lncRNAs play crucial roles in the initiation and progression of various malignant tumors. VIM-AS1 RNA is an lncRNA that transcribes from a shared bidirectional promoter with vimentin mRNA and its function in cancer cells is largely unknown. This study assessed the clinical significance of VIM-AS1 expression in colorectal cancer (CRC). We found that the VIM-AS1 transcript was significantly upregulated in high-grade, lymph node metastasis and vascular invasion tumors. Loss-of-function experiments revealed that the downregulation of VIM-AS1 could inhibit tumor cell proliferation by inducing apoptosis, cellular senescence and arresting the cell cycle...
April 7, 2018: European Journal of Cell Biology
Z-G Gu, G-H Shen, J-H Lang, W-X Huang, Z-H Qian, J Qiu
OBJECTIVE: To investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODS: The expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells...
March 2018: European Review for Medical and Pharmacological Sciences
Zhenqiang Sun, Jinbo Liu, Chen Chen, Quanbo Zhou, Shuaixi Yang, Guixian Wang, Junmin Song, Zhen Li, Zhiyong Zhang, Jizhong Xu, Xiantao Sun, Yuan Chang, Weitang Yuan
Colorectal cancer (CRC) is one of the most common malignancies in the world. Easier recurrence and metastasis is the main cause of mortality in CRC patients, and the markers applied for diagnosis and treatment of CRC is still urgently needed to early diagnose and evaluate therapeutic effect. Long noncoding RNA (lncRNA) is a class of noncoding RNA that the length is more than 200 nucleotides. With the development of sequencing technique about transcriptome, increasing lncRNAs are focused on their function and mechanism related to the nosogenesis and pathology of CRC...
March 26, 2018: Cellular Physiology and Biochemistry
Weiwei Zhou, Lei Wang, Yu Miao, Rongge Xing
Aim: To explore the expression and clinical significance of long noncoding RNA (lncRNA) gastric cancer-associated transcript 3 (GACAT3) in human colorectal cancer (CRC). Methods: Expression of GACAT3 in CRC tissues and cell lines was measured using quantitative real-time PCR. CCK-8 and colony formation assays were used to assess the effect of GACAT3 on CRC cell line proliferation. Transwell invasion and migration assays were performed to detect the effect of GACAT3 on CRC cell line invasion and migration...
2018: OncoTargets and Therapy
Xiaoli Wang, Fen Liu, Xiaoyuan Liu, Fang Wang, Xiaoyu Liao, Yuanbin Chen, Yong Mao, Dong Hua, Xiaosong Ge
Background: Long non-coding RNAs (lncRNAs) have essential regulatory function, yet their roles in colorectal cancer (CRC) are not well understood. Materials and methods: Microarray was applied to detect lncRNAs expression profiles in tumor tissues, liver metastasis and paired adjacent normal tissues of CRC. And using RT-PCR to verify chip results. Results: A total of 10 680 lncRNAs demonstrated differential expressions (fold change ≥2) between tumor tissues and adjacent normal tissues; furthermore there were 2970 lncRNAs, which showed different expression level between CRC tissues with liver metastasis and adjacent normal tissues...
March 26, 2018: Japanese Journal of Clinical Oncology
Chaoqun Cen, Jian Li, Jingjing Liu, Mingshi Yang, Tianyi Zhang, Yu Zuo, Changwei Lin, Xiaorong Li
Background: Abnormal expression of long non-coding RNA (lncRNAs) often facilitates unrestricted growth of cancer cells. Long intergenic non-protein coding RNA 1510, an enhancer lncRNA (LINC01510), a lncRNA enhancer is upregulated in colorectal cancer (CRC), and its expression might relate to MET as revealed by lncRNA microarray data. However, the potential biological role of LINC01510 and its regulatory mechanism in CRC remain unclear. Therefore, we investigated the involvement of LINC01510 in the proliferation of CRC cells...
2018: Cancer Cell International
Xiu-Liang Xia, Dong Xue, Ting-Hai Xiang, Huai-Yong Xu, De-Kun Song, Pei-Guang Cheng, Jian-Qiang Wang
The clinical significance and essential role of long non-coding RNA colorectal neoplasia differentially expressed (lncRNA CRNDE) have been well illuminated in various cancers. However, the function of CRNDE in intrahepatic cholangiocarcinoma (IHCC) has not been reported at present. The aim of the present study was to investigate the role of CRNDE in IHCC. Firstly, the relative expression of CRNDE was observed to be upregulated in IHCC cell lines and tissues. And high CRNDE expression was statistically associated with IHCC differentiation grade, lymph node metastasis, tumor-nodes-metastasis (TNM) stage and size...
April 2018: Oncology Letters
Zhengting Chen, Xinyi Cai, Li Chang, Yaoxiong Xia, Li Wang, Yu Hou, Lan Li, Dingguo Pan, Furong Li, Shan Liu, Wei Xiong, Wenhui Li
Concurrent radiotherapy and chemotherapy is a widely used, comprehensive treatment for rectal cancer. By studying the impact of concurrent chemoradiotherapy on the invasion and migration of colorectal cancer (CRC) cells and researching the associated molecular mechanisms, the present study aimed to provide a novel method to improve the therapeutic effect of this treatment against CRC. Human HCT116 and HT29 CRC cells were simultaneously treated with 4 Gy of 6 MV X-rays and 10 µmol/l 5-fluorouracil to establish a residual cell model...
April 2018: Oncology Letters
Mu-Lin Liu, Qiao Zhang, Xiao Yuan, Long Jin, Li-Li Wang, Tao-Tao Fang, Wen-Bin Wang
AIM: To investigate the role of long noncoding RNA (lncRNA) RP4 in colorectal cancer. METHODS: Lentivirus-mediated lncRNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lncRNA RP4 mechanism involved assessment of the association of its expression with miR-7-5p and the SH3GLB1 gene...
March 7, 2018: World Journal of Gastroenterology: WJG
Zhiming Shan, Na An, Jielin Qin, Junmei Yang, Hongqi Sun, Wei Yang
Substantial evidence has demonstrated the involvement of long non-coding RNAs (lncRNAs) in the development and progression of colorectal cancer (CRC) via their regulation on cancer cell proliferation, apoptosis, invasion and metastasis pathways. The current study aimed to understand the role of lncRNA Linc00675 in the progression and metastasis of CRC and to identify the potential lncRNA-miRNA interactions and signaling pathways underlying the mechanisms of action of Linc00675 in CRC. Our data firstly demonstrated the down-regulation of Linc00675 in both CRC cells and clinical CRC tissues...
March 7, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Kuo-Wang Tsai, Yi-Hao Lo, Hsuan Liu, Chung-Yu Yeh, You-Zuo Chen, Chao-Wen Hsu, Wei-Shone Chen, Jui-Ho Wang
BACKGROUND: Colorectal cancer (CRC) is one of the most common cancers and causes of cancer-related death worldwide. In patients with CRC, metastasis is a crucial problem that leads to treatment failure and is the primary cause of the lethality of colon cancer. Long noncoding RNAs (lncRNAs) have recently emerged as critical molecules in the development, cell growth, apoptosis, and metastasis of CRC. METHOD: We investigated the transcriptome profiles of human lncRNAs in the primary tumor tissues and in the corresponding normal mucosa of two patients with CRC by using a microarray approach...
March 10, 2018: Molecular Cancer
Chaoyu Wang, Xin Zhu, Chibin Pu, Xuan Song
Emerging evidence indicates that the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) is associated with tumourigenesis in various types of cancer. However, its specific effects on the proliferation, invasion and metastasis of colorectal cancer (CRC) are still poorly understood. The present study aimed to investigate PVT1 expression in CRC and explore its role in CRC pathogenesis. The reverse transcriptase‑quantitative polymerase chain reaction (RT‑qPCR) technique was used to assess PVT1 expression in CRC cell lines...
March 1, 2018: Molecular Medicine Reports
Wan-Cheng Xiong, Na Han, Nan Wu, Ke-Lei Zhao, Chen Han, Hui-Xin Wang, Guan-Fang Ping, Peng-Fei Zheng, Hailong Feng, Lei Qin, Peng He
Long noncoding RNAs (lncRNAs) are dysregulated in many diseases. MicroRNA-101 (miR-101) functions as a tumor suppressor by directly targeting ZEB1 in various cancers. However, the potential mechanism of lncRNA ZEB1-AS1 and miR-101/ZEB1 axis in CRC remains unknown. In this study, we further investigated the potential interplay between miR-101/ZEB1 axis and lncRNA ZEB1-AS1 in colorectal cancer (CRC). Results showed that ZEB1-AS1 was upregulated in CRC tissues and cells. MiR-101 was downregulated in CRC tissues and negatively correlated with ZEB1-AS1 and ZEB1 expression levels in CRC...
2018: American Journal of Translational Research
Y Zhao, Z-S Qin, Y Feng, X-J Tang, T Zhang, L Yang
OBJECTIVE: Colorectal cancer (CRC) is one of the most frequent malignant tumors worldwide. The connection between lncRNAs expression and CRC development has not been well identified in the recent literature. This study focuses on the role of lncRNA-SNHG1 on CRC progression and development. The quantitative Real-time PCR (qRT-PCR) assay was conducted to identify the expression level of small nucleolar RNA host gene 1 (SNHG1). PATIENTS AND METHODS: Cell proliferation and viability were examined by 3-(4,5)-dimethylthiazol(-z-y1)-3,5-diphenyl tetrazoliumbromide (MTT assay) and colony formation assay...
February 2018: European Review for Medical and Pharmacological Sciences
Min Li, Zehua Bian, Surui Yao, Jia Zhang, Guoying Jin, Xue Wang, Yuan Yin, Zhaohui Huang
Long non-coding RNAs (lncRNAs) have been shown to play important roles in tumor formation and development. Small nucleolar RNA host gene 6 (SNHG6) is a recently identified cancer-related lncRNA, and its role in colorectal cancer (CRC) remains to be explored. The aim of this study was to evaluate the expression and function of SNHG6 in CRC. The expression of SNHG6 was detected by real time quantitative RT-PCR (qRT-PCR) in 74 CRC tissues and matched noncancerous tissues (NCTs). Relationships between the expression levels of SNHG6 and various clinicopathological features were analyzed by Chi-square test...
January 3, 2018: Pathology, Research and Practice
Xingxiang Liu, Lin Cui, Dong Hua
We aimed to investigate the significant role of long non-coding RNA X Inactive Specific Transcript (XIST) in regulatingtumor metastasis in colorectal cancer (CRC), as well as its possible mechanism. Expression of lncRNA XIST in CRC tissues and CRC cells was detected. CRC cells were transfected with pc-XIST, blank control si-XIST or si-control, and then the effects of lncRNA XIST on CRC cell migration and invasion were investigated, along with the interaction between lncRNA XIST and miR-137. LncRNA XIST was upregulated in CRC tissues...
March 1, 2018: Oncology Research
L Chen, W Zhang, D Y Li, X Wang, Y Tao, Y Zhang, C Dong, J Zhao, L Zhang, X Zhang, J Guo, X Zhang, Q Liao
Colorectal cancer (CRC), one of the common malignant cancers in the world, is caused by accumulated alterations of genetic and epigenetic factors over a long period of time. Along with that protein-coding genes being identified as oncogenes or tumor suppressors in CRC, a number of lncRNAs have also been found to be associated with CRC. Considering the important regulatory role of lncRNAs, the first goal of this study was to identify CRC-associated lncRNAs from a public database. One such lncRNA, LINC00472, was verified to be downregulated in CRC cell lines and cancer tissues compared with adjacent tissues...
February 28, 2018: Clinical Genetics
Jie Zhang, Xiao-Yan Li, Ping Hu, Yuan-Sheng Ding
Previous study indicates that long noncoding RNA NORAD could serve as a competing endogenous RNA to pancreatic cancer metastasis. However, its role in colorectal cancer requires to be investigated. In the present study, we found that the expression of NORAD was significantly upregulated in colorectal cancer (CRC) tissues. Furthermore, the expression of NORAD was positively related with CRC metastasis and patients' poor prognosis. Knockdown of NORAD markedly inhibited CRC cell proliferation, migration and invasion but induced cell apoptosis in vitro...
February 22, 2018: Oncology Research
Miganoosh Simonian, Mohammadreza Sharifi, Reza Nedaeinia, Meysam Mosallaie, Sharifeh Khosravi, Amir Avan, Majid Ghayour-Mobarhan, Hadi Bagheri, Rasoul Salehi
Background: Both microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) have been shown to have a critical role in the regulation of cellular processes such as cell growth and apoptosis, as well as cancer progression and metastasis. lncRNAs are aberrantly expressed in many diseases including cancer. Although it is well known that miRNAs can target a large number of protein-coding genes, little is known whether miRNAs can also target lncRNAs. In the present study, we determine whether miR-21 can regulate lncRNA cancer susceptibility candidate 2 (CASC2) in colorectal cancer...
2018: Advanced Biomedical Research
Jinfang Xue, Liya Liao, Fang Yin, Haoyu Kuang, Xiaojun Zhou, Yanan Wang
BACKGROUND: LncRNAs are involved in the metastasis and recurrence of human tumors, including colorectal cancer (CRC). We previously reported that lncRNA AB073614 promotes tumor proliferation and metastasis and predicted a poor clinical outcome of CRC patients. Herein, we investigated the underlying mechanism of lncRNA AB073614-related metastasis in CRC. MATERIAL AND METHODS: The expression of lncRNA AB073614 in CRC tissues were evaluated by quantitative real-time PCR (qRT-PCR)...
January 26, 2018: Cancer Biomarkers: Section A of Disease Markers
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