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https://www.readbyqxmd.com/read/28820183/technique-of-retinal-gene-therapy-delivery-of-viral-vector-into-the-subretinal-space
#1
K Xue, M Groppe, A P Salvetti, R E MacLaren
PurposeSafe and reproducible delivery of gene therapy vector into the subretinal space is essential for successful targeting of the retinal pigment epithelium (RPE) and photoreceptors. The success of surgery is critical for the clinical efficacy of retinal gene therapy. Iatrogenic detachment of the degenerate (often adherent) retina in patients with hereditary retinal degenerations and small volume (eg, 0.1 ml) subretinal injections pose new surgical challenges.MethodsOur subretinal gene therapy technique involved pre-operative planning with optical coherence tomography (OCT) and autofluorescence (AF) imaging, 23 G pars plana vitrectomy, internal limiting membrane staining with Membrane Blue Dual (DORC BV, Zuidland, Netherlands), a two-step subretinal injection using a 41 G Teflon tipped cannula (DORC) first with normal saline to create a parafoveal bleb followed by slow infusion of viral vector via the same self-sealing retinotomy...
August 18, 2017: Eye
https://www.readbyqxmd.com/read/28816729/retina-expansion-technique-for-macular-hole-apposition-report-2-efficacy-closure-rate-and-risks-of-a-macular-detachment-technique-to-close-large-full-thickness-macular-holes
#2
Roger Wong, Catherine Howard, Giancarlo Dellʼaversana Orobona
PURPOSE: To describe the safety and efficacy of a technique to close large thickness macular holes. METHODS: A consecutive retrospective interventional case series of 16 patients with macular holes greater than 650 microns in "aperture" diameter were included. The technique involves vitrectomy, followed by internal limiting membrane peeling. The macula is detached using subretinal injection of saline. Fluid-air exchange is performed to promote detachment and stretch of the retina...
August 14, 2017: Retina
https://www.readbyqxmd.com/read/28814521/hiv-fusion-in-dendritic-cells-mainly-occurs-at-the-surface-and-is-limited-by-low-cd4-levels
#3
Lise Chauveau, Daniel Aaron Donahue, Blandine Monel, Francoise Porrot, Timothée Bruel, Lea Richard, Nicoletta Casartelli, Olivier Schwartz
HIV-1 poorly infects monocyte-derived dendritic cells (MDDCs). This is in a large part due to SAMHD1, which restricts viral reverse transcription. Pseudotyping HIV-1 with VSV-G strongly enhances infection, suggesting that earlier steps of viral replication, including fusion, are also inefficient in MDDCs. The site of HIV-1 fusion remains controversial and may depend on the cell type, with reports indicating that it occurs at the plasma membrane or contrarily, in an endocytic compartment. Here, we examined the pathways of HIV-1 entry in MDDCs...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28811935/pars-plana-vitrectomy-with-internal-limiting-membrane-peeling-in-traumatic-macular-hole-14-perfluoropropane-c3f8-versus-silicone-oil-tamponade
#4
Ashraf Bor'i, Mahmoud A Al-Aswad, Ahmed Abdelwahab Saad, Dina Hamada, Ashraf Mahrous
PURPOSE: To evaluate the outcome of 23 G PPV and ILM peeling with 14% C3F8 compared with silicone oil tamponade in cases of TMHs without spontaneous closure. METHODS: A retrospective comparative study included 33 eyes with TMHs; 7 eyes healed spontaneously, and the remaining 26 eyes have been treated with PPV and ILM peeling. Silicone oil was used as a tamponade for children or adults who refused to adopt face-down position (10 cases). In all other cases (16 cases), 14% C3F8 was used...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/28796683/vaginal-cleansing-before-cesarean-delivery-a-systematic-review-and-meta-analysis
#5
Claudia Caissutti, Gabriele Saccone, Fabrizio Zullo, Johanna Quist-Nelson, Laura Felder, Andrea Ciardulli, Vincenzo Berghella
OBJECTIVE: To assess the efficacy of vaginal cleansing before cesarean delivery in reducing postoperative endometritis. DATA SOURCES: MEDLINE, Ovid, EMBASE, Scopus, Clinicaltrials.gov, and Cochrane Library were searched from their inception to January 2017. METHODS OF STUDY SELECTION: Selection criteria included all randomized controlled trials comparing vaginal cleansing (ie, intervention group) with a control group (ie, either placebo or no intervention) in women undergoing cesarean delivery...
August 4, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28796147/surgical-outcomes-in-eyes-with-extremely-high-myopia-for-macular-hole-without-retinal-detachment
#6
Tsung-Tien Wu, Ya-Hsin Kung, Chih-Yao Chang, Shu-Ping Chang
PURPOSE: The authors evaluated surgical outcomes in eyes with extremely high myopia for macular hole (MH) without retinal detachment. METHODS: In this retrospective study, 14 eyes with axial lengths of ≥30.0 mm underwent vitrectomy and internal limiting membrane (ILM) peeling with or without inverted ILM flap insertion for MH without retinal detachment (October 2009-June 2016). Outcome measures were MH closure confirmed by optical coherence tomography, best-corrected visual acuity, and complications...
August 8, 2017: Retina
https://www.readbyqxmd.com/read/28796146/hole-door-sign-a-novel-intraoperative-optical-coherence-tomography-feature-predicting-macular-hole-closure
#7
Vinod Kumar, Bhupendra Yadav
PURPOSE: To describe a novel intraoperative finding during pars plana vitrectomy for macular hole using operating microscope-integrated spectral domain optical coherence tomography that predicts the closure of macular hole. METHODS: Twenty-five eyes of 25 patients with macular hole, who underwent 25-gauge pars plana vitrectomy over a period of 16 months at a tertiary eye care center by a single surgeon, were recruited in this retrospective interventional study. All eyes were assessed with intraoperative spectral domain optical coherence tomography before and after internal limiting membrane peeling...
August 8, 2017: Retina
https://www.readbyqxmd.com/read/28794434/protective-role-of-cellular-prion-protein-against-tnf%C3%AE-mediated-inflammation-through-tace-%C3%AE-secretase
#8
Juliette Ezpeleta, François Boudet-Devaud, Mathéa Pietri, Anne Baudry, Vincent Baudouin, Aurélie Alleaume-Butaux, Nathalie Dagoneau, Odile Kellermann, Jean-Marie Launay, Benoit Schneider
Although cellular prion protein PrP(C) is well known for its implication in Transmissible Spongiform Encephalopathies, its functions remain elusive. Combining in vitro and in vivo approaches, we here show that PrP(C) displays the intrinsic capacity to protect neuronal cells from a pro-inflammatory TNFα noxious insult. Mechanistically, PrP(C) coupling to the NADPH oxidase-TACE α-secretase signaling pathway promotes TACE-mediated cleavage of transmembrane TNFα receptors (TNFRs) and the release of soluble TNFR, which limits the sensitivity of recipient cells to TNFα...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28791069/outcome-of-macular-hole-surgery-in-bietti-crystalline-dystrophy
#9
Ramin Nourinia, Mohammad Hossein Dehghan, Sahba Fekri
PURPOSE: To describe a 42-year-old man, a known case of Bietti crystalline dystrophy who underwent surgery for unilateral full thickness macular hole. CASE REPORT: Clinical features, color fundus photographs, and optical coherence tomography, electroretinography, and electrooculography findings of the patient are reported. His visual acuity improved from counting fingers to 20/50 after pars plana deep vitrectomy with internal limiting membrane (ILM) peeling and gas injection...
July 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28782502/society-for-maternal-fetal-medicine-smfm-consult-series-43-hepatitis-c-in-pregnancy-screening-treatment-and-management
#10
Brenna L Hughes, Charlotte M Page, Jeffrey A Kuller
In the United States, 1-2.5% of pregnant women are infected with hepatitis C virus (HCV), which carries an approximately 5% risk of transmission from mother to infant. HCV can be transmitted to the infant in utero or during the peripartum period, and infection during pregnancy is associated with increased risk of adverse fetal outcomes, including fetal growth restriction and low birth weight. The purpose of this document is to discuss the current evidence regarding HCV in pregnancy and to provide recommendations on screening, treatment, and management of this disease during pregnancy...
August 3, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28777226/is-the-optic-nerve-head-structure-impacted-by-a-diagnostic-lumbar-puncture-in-humans
#11
Muriel Poli, Philippe Denis, Eric Sellem, Ludwig-Serge Aho-Glélé, Alain M Bron
PURPOSE: To assess in vivo whether diagnostic lumbar puncture (LP) is followed by optic nerve head (ONH) and parapapillary anatomical changes in normal human eyes. METHODS: Prospective, single-center, observational case series. ONH structures (prelaminar tissue surface, anterior surface of the lamina cribrosa, central retinal vessels) and parapapillary structures (internal limiting membrane, posterior surfaces of retinal nerve fiber layer and Bruch membrane/retinal pigment epithelium complex, Bruch membrane opening, posterior surface of the choroid) were quantitatively evaluated by means of swept-source OCT (Triton Ver...
August 2, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28768260/vitrectomy-inner-limiting-membrane-peel-and-gas-tamponade-in-the-management-of-traumatic-paediatric-macular-holes-a-case-series-of-13-patients
#12
Nicholas Brennan, Ian Reekie, Anthony P Khawaja, Nikolaos Georgakarakos, Eric Ezra
PURPOSE: To review the outcomes of pars plana vitrectomy, internal limiting membrane (ILM) peel, and gas tamponade in the management of traumatic paediatric macular holes. METHODS: Retrospective case series of children undergoing vitrectomy, ILM peel, and gas tamponade for traumatic macular hole between March 2007 and July 2014. Main outcome measures were postoperative visual acuity at 3 and 12 months, anatomic closure rate, and surgical complications. RESULTS: Anatomic macular hole closure was achieved in 12 (92...
July 29, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28767555/foveal-avascular-zone-area-after-internal-limiting-membrane-peeling-for-epiretinal-membrane-and-macular-hole-compared-with-that-of-fellow-eyes-and-healthy-controls
#13
Kazuyuki Kumagai, Mariko Furukawa, Tetsuyuki Suetsugu, Nobuchika Ogino
PURPOSE: To measure the foveal avascular zone (FAZ) area after internal limiting membrane (ILM) peeling and to determine the factors significantly correlated with the FAZ area. METHODS: This was a retrospective, observational, and cross-sectional study. The affected and normal fellow eyes of 102 patients with unilateral macular diseases and 169 healthy subjects were studied. The patients underwent successful vitrectomy with internal limiting membrane peeling for an epiretinal membrane (n = 56) or a macular hole (n = 46)...
August 1, 2017: Retina
https://www.readbyqxmd.com/read/28764684/intraoperative-optical-coherence-tomography-assisted-analysis-of-pars-plana-vitrectomy-for-retinal-detachment-in-morning-glory-syndrome-a-case-report
#14
Lyubomyr M Lytvynchuk, Carl G Glittenberg, Siamak Ansari-Shahrezaei, Susanne Binder
BACKGROUND: The pathogenesis of non-rhegmatogenous retinal detachment (non-RRD) associated with morning glory syndrome (MGS) is not established, as well as best surgical approach to treat RD. Our purpose was to analyse intraoperative optical coherence tomography data (iOCT) in all steps of pars plana vitrectomy (PPV) for non-RRD in MGS, in order to follow pathophysiological aspects of the disease and to understand the tissues behaviour during surgical workflow. CASE PRESENTATION: Intraoperative spectral domain optical coherent tomography (iSD-OCT) assisted PPV using Rescan 700 (Carl Zeiss Meditech, Jena, Germany) with epiretinal membrane (ERM) and internal retinal membrane (ILM) peeling, and air endotamponade was performed on the only eye of a 21 years old female with non-RRD associated with MGS...
August 1, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28758654/particle-size-affects-the-cytosolic-delivery-of-membranotropic-peptide-functionalized-platinum-nanozymes
#15
Daniela Guarnieri, Pietro Melone, Mauro Moglianetti, Roberto Marotta, Paolo A Netti, Pier Paolo Pompa
Delivery of therapeutic agents inside the cytosol, avoiding the confinement in endo-lysosomal compartments and their degradative environment, is one of the key targets of nanomedicine to gain the maximum remedial effects. Current approaches based on cell penetrating peptides (CPPs), despite improving the cellular uptake efficiency of nanocarriers, have shown controversial results in terms of intracellular localization. To elucidate the delivery potential of CPPs, in this work we analyzed the role of the particle size in influencing the ability of a membranotropic peptide, namely gH625, to escape the endo-lysosomal pathway and deliver the particles in the cytosol...
July 31, 2017: Nanoscale
https://www.readbyqxmd.com/read/28755363/direct-extraction-and-purification-of-recombinant-membrane-proteins-from-pichia-pastoris-protoplasts
#16
Lucie Hartmann, Estelle Metzger, Noémie Ottelard, Renaud Wagner
In the past decade, the methylotrophic yeast Pichia pastoris has proved to be one of the most efficient systems for mass production of recombinant eukaryotic membrane proteins (MPs), leading to the crystallization and structure determination for a variety of them. The actual overexpression of functional MPs achieved with this system is, however, often accompanied by the formation of a variable but significant proportion of misfolded and/or aggregated proteins that are co-extracted and co-purified during the purification process...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28753983/outcome-of-full-thickness-macular-hole-surgery-in-choroideremia
#17
Mays Talib, Leonoor S Koetsier, Robert E MacLaren, Camiel J F Boon
The development of a macular hole is relatively common in retinal dystrophies eligible for gene therapy such as choroideremia. However, the subretinal delivery of gene therapy requires an uninterrupted retina to allow dispersion of the viral vector. A macular hole may thus hinder effective gene therapy. Little is known about the outcome of macular hole surgery and its possible beneficial and/or adverse effects on retinal function in patients with choroideremia. We describe a case of a unilateral full-thickness macular hole (FTMH) in a 45year-old choroideremia patient (c...
July 21, 2017: Genes
https://www.readbyqxmd.com/read/28747253/ordered-mesoporous-carbon-as-sorbent-for-the-extraction-of-n-nitrosamines-in-wastewater-and-swimming-pool-water
#18
Maryam Lashgari, Yadollah Yamini, Chanbasha Basheer, Hian Kee Lee
The analysis and determination of N-nitrosamines (NAs) in water samples are challenging and demanding. In this study, a simple, reliable, and practical methodology is reported for the quantitative determination by gas chromatography-tandem mass spectrometry with electron impact ionization (EI) and triple quadrupole analyzer (GC-EI-MS/MS) of eight NAs after micro-solid-phase extraction (μ-SPE) from wastewater and swimming pool water. Thirty milligram of an ordered mesoporous carbonaceous material, oxidative surface-modified CMK-3, enclosed within a porous polypropylene membrane bag, were used as sorbent for μ-SPE...
July 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28745657/-surgical-treatment-of-rhegmatogenous-retinal-detachments-with-high-risk-of-proliferative-vitreoretinopathy
#19
L A Akhundova, E M Kasimov
AIM: to analyze the effect of internal limiting membrane (ILM) peeling in patients undergoing 23-gauge pars plana vitrectomy for the treatment of rhegmatogenous retinal detachment (RRD) with a high risk of proliferative vitreoretinopathy (PVR). MATERIAL AND METHODS: This was a prospective consecutive study of 231 eyes of 227 patients. All eyes underwent vitrectomy with silicone oil/gas tamponade for RRD with a high risk of PVR: in 42 eyes the ILM was peeled (group 1) and in the remaining 189 eyes - was not (group 2)...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28739320/immediate-implant-placement-into-posterior-sockets-with-or-without-buccal-bone-dehiscence-defects-a-retrospective-cohort-study
#20
Chen Hu, Ting Gong, Weimin Lin, Quan Yuan, Yi Man
OBJECTIVES: To evaluate bone reconstruction and soft tissue reactions at immediate implants placed into intact sockets and those with buccal bone dehiscence defects. METHODS: Fifty-nine internal connection implants from four different manufacturers were immediately placed in intact sockets(non-dehiscence group, n=40), and in alveoli with buccal bone dehiscence defects: 1) Group 1(n= N10), the defect depth measured 3-5 mm from the gingival margin. 2) Group 2(n=9), the depth ranged from 5mm to 7mm...
July 21, 2017: Journal of Dentistry
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