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Internal limiting membrane

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https://www.readbyqxmd.com/read/29238614/recurrence-rate-and-need-for-reoperation-after-surgery-with-or-without-internal-limiting-membrane-removal-for-the-treatment-of-the-epiretinal-membrane
#1
Fernando José De Novelli, Mauro Goldbaum, Mario Luiz Ribeiro Monteiro, Fabio Bom Aggio, Mario Junqueira Nóbrega, Walter Yukihiko Takahashi
Purpose: To compare the recurrence rate and need for reoperation after epiretinal membrane surgery with and without removal of the internal limiting membrane. Methods: In this retrospective study, 125 patients operated for epiretinal membrane removal were evaluated, with a minimum 6-month follow-up. Removal of the epiretinal membrane (ERM) was performed in 78 patients, while 47 had removal of the epiretinal membrane associated with internal limiting membrane peeling (ERM + ILM)...
2017: International Journal of Retina and Vitreous
https://www.readbyqxmd.com/read/29237145/current-practices-in-the-management-of-congenital-diaphragmatic-hernia-patients-requiring-extracorporeal-membrane-oxygenation-results-of-an-international-survey-of-pediatric-surgeons
#2
Sophia Abdulhai, Ian C Glenn, Neil L McNinch, Todd A Ponsky, Avraham Schlager
INTRODUCTION: There is little consensus on optimal management for congenital diaphragmatic hernia extracorporeal membrane oxygenation (CDH ECMO) patients. Meaningful comparisons of the various approaches have been limited due to the low number of cases in institutions. In addition, the multidisciplinary reliance and rigid institutional framework of ECMO serve to further limit exposure to alternative practices. The goal of this study is to survey the international pediatric surgery community to describe the current practice trends...
December 13, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/29235973/linear-array-of-multi-substrate-tracts-for-simultaneous-assessment-of-cell-adhesion-migration-and-differentiation
#3
Ricardo A Moreno-Rodriguez, Edward L Krug, Leticia Reyes, Roger R Markwald
Cell migration, which is central to a wide variety of life processes, involves integration of the extracellular matrix (ECM) with the internal cytoskeleton and motor proteins via receptors spanning the plasma membrane. Cell migration can be induced by a variety of signals, including gradients of external soluble molecules, differences in ECM composition, or electrical gradients. Current in vitro methods to study cell migration only test one substrate at a time. Here, we present a method for assessing cell adhesion, migration, and differentiation in up to 20 different test conditions simultaneously, using only minute amounts of target substrate...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29232338/internal-limiting-membrane-peeling-versus-inverted-flap-technique-for-treatment-of-full-thickness-macular-holes-a-comparative-study-in-a-large-series-of-patients
#4
Stanislao Rizzo, Ruggero Tartaro, Francesco Barca, Tomaso Caporossi, Daniela Bacherini, Fabrizio Giansanti
BACKGROUND: The inverted flap (IF) technique has recently been introduced in macular hole (MH) surgery. The IF technique has shown an increase of the success rate in the case of large MHs and in MHs associated with high myopia. This study reports the anatomical and functional results in a large series of patients affected by MH treated using pars plana vitrectomy and gas tamponade combined with internal limiting membrane (ILM) peeling or IF. METHODS: This is a retrospective, consecutive, nonrandomized comparative study of patients affected by idiopathic or myopic MH treated using small-gauge pars plana vitrectomy (25- or 23-gauge) between January 2011 and May 2016...
December 8, 2017: Retina
https://www.readbyqxmd.com/read/29223663/patch-esophagoplasty-using-an-in-body-tissue-engineered-collagenous-connective-tissue-membrane
#5
Hiroomi Okuyama, Satoshi Umeda, Yuichi Takama, Takeshi Terasawa, Yasuhide Nakayama
AIM: Although many approaches to esophageal replacement have been investigated, these efforts have thus far only met limited success. In-body-tissue-engineered connective tissue tubes have been reported to be effective as vascular replacement grafts. The aim of this study was to investigate the usefulness of an In-body-tissue-engineered collagenous connective tissue membrane, "Biosheet", as a novel esophageal scaffold in a beagle model. METHODS: We prepared Biosheets by embedding specially designed molds into subcutaneous pouches in beagles...
November 13, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29220817/infrared-spectroscopy-of-flavones-and-flavonols-reexamination-of-the-hydroxyl-and-carbonyl-vibrations-in-relation-to-the-interactions-of-flavonoids-with-membrane-lipids
#6
Goran Baranović, Suzana Šegota
Detailed vibrational assignments for twelve flavonoids (seven flavones (flavone, 3- and 5-hydroxyflavone, chrysin, apigenin, fisetin and luteolin) and five flavonols (galangin, kaempferol, quercetin, morin and myricetin)) have been made based on own and reported experimental data and calculations at the B3LYP/6-31+G(d,p) level of theory. All the molecules are treated in a uniform way by using the same set of redundancy-free set of internal coordinates. A generalized harmonic mode mixing is used to corroborate the vibrational characteristics of this important class of molecules...
December 2, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29219961/measurement-of-full-thickness-macular-hole-size-using-en-face-optical-coherence-tomography
#7
E Philippakis, M Legrand, M El Sanharawi, A Erginay, A Couturier, R Tadayoni
PurposeTo assess the feasibility and interest of measuring macular hole (MH) size using en face optical coherence tomography (OCT) compared with manual diameter measurements on B-scans.MethodsAmong our previously published series of 100 patients operated for primary MH, patients whose images were acquired with Cirrus 5000 HD-OCT (Carl Zeiss Meditec) with a quality signal strength >5/10 were included. Three segmentations (internal limiting membrane, horizontal, and retinal pigment epithelium (RPE)) were compared for obtaining the most appropriate en face image...
December 8, 2017: Eye
https://www.readbyqxmd.com/read/29215533/surgical-removal-of-epiretinal-membrane-with-and-without-removal-of-internal-limiting-membrane-comparative-study-of-visual-acuity-features-of-optical-coherence-tomography-and-recurrence-rate
#8
Fernando J De Novelli, Mauro Goldbaum, Mario L R Monteiro, Fabio Bom Aggio, Walter Y Takahashi
PURPOSE: To study and compare visual acuity, foveal thickness, outer limiting layer, ellipsoid zone, and recurrence rate in patients undergoing removal of the epiretinal membrane with and without the removal of the internal limiting membrane (ILM). METHODS: Sixty-three patients who had the epiretinal membrane removed by a single surgeon were randomly assigned into 2 groups: Group 1 without additional removal of the ILM and Group 2 with removal of the ILM. Patients were followed up and evaluated at the first month, third month, and sixth month, postoperatively...
December 5, 2017: Retina
https://www.readbyqxmd.com/read/29214314/quantitative-fundus-autofluorescence-in-pseudoxanthoma-elasticum
#9
Martin Gliem, Philipp L Müller, Johannes Birtel, Myra B McGuinness, Robert P Finger, Philipp Herrmann, Doris Hendig, Frank G Holz, Peter Charbel Issa
Purpose: To quantify lipofuscin-associated fundus autofluorescence in patients with pseudoxanthoma elasticum (PXE), a model disease for Bruch's membrane pathology. Methods: In this prospective, monocenter, cross-sectional case-control study, 49 patients with PXE (mean age: 46 years, range 18-62) underwent quantitative fundus autofluorescence (qAF) imaging with a modified scanning laser ophthalmoscope containing an internal fluorescent reference for normalization of images...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29210940/internal-limiting-membrane-peeling-in-macular-hole-surgery-why-when-and-how
#10
Irini P Chatziralli, Panagiotis G Theodossiadis, David H W Steel
PURPOSE: To review the current rationale for internal limiting membrane (ILM) peeling in macular hole (MH) surgery and to discuss the evidence base behind why, when, and how surgeons peel the ILM. METHODS: Review of the current literature. RESULTS: Pars plana vitrectomy is an effective treatment for idiopathic MH, and peeling of the ILM has been shown to improve closure rates and to prevent postoperative reopening. However, some authors argue against ILM peeling because it results in a number of changes in retinal structure and function and may not be necessary in all cases...
November 23, 2017: Retina
https://www.readbyqxmd.com/read/29208490/fluorescence-enlightens-rnd-pump-activity-and-the-intrabacterial-concentration-of-antibiotics
#11
Muriel Masi, Estelle Dumont, Julia Vergalli, Jelena Pajovic, Matthieu Réfrégiers, Jean-Marie Pagès
To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric method using intrinsic tryptophan fluorescence as an internal standard, as well as a complementary microfluorimetric assay following time-course accumulation in intact individual cells, have been developed...
December 2, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/29200823/six-month-postoperative-outcomes-of-intraoperative-oct-guided-surgical-cystotomy-for-refractory-cystoid-macular-edema-in-diabetic-eyes
#12
Yuichi Asahina, Naoko Tachi, Yumi Asahina, Kayoko Yoshimura, Yoshiki Ueta, Yoshihiro Hashimoto
Purpose: This study evaluated the outcomes of surgical cystotomy for recurrent diabetic cystoid macular edema (CME). Patients and methods: We analyzed 20 eyes with a clinical diagnosis of diabetic retinopathy and refractory CME. Release of vitreoretinal adhesion, epiretinal membrane (ERM) and internal limiting membrane (ILM) peeling and cystotomy guided by intraoperative optical coherence tomography (iOCT) were performed in every patient. Pars plana vitrectomy was also performed in 17 patients, 11 of whom also underwent lensectomy and intraocular lens implantation...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29193962/cell-penetrating-polymers-containing-guanidinium-trigger-apoptosis-in-human-hepatocellular-carcinoma-cells-unless-conjugated-to-a-targeting-n-acetyl-galactosamine-block
#13
Zhe Tan, Yogesh K Dhande, Theresa M Reineke
A series of 3-guanidinopropyl methacrylamide (GPMA)-based polymeric gene delivery vehicles were developed via aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymers have been evaluated for their cellular internalization ability, transfection efficiency, and cytotoxicity. Two homopolymers: P(GPMA20), P(GPMA34), were synthesized to study the effect of guanidium polymer length on delivery efficiency and toxicity. In addition, an N-acetyl-d-galactosamine (GalNAc)-based hydrophilic block was incorporated to produce diblock polymers, which provides a neutral hydrophilic block that sterically protects plasmid-polymer complexes (polyplexes) from colloidal aggregation and aids polyplex targeting to hepatocytes via binding to asialoglycoprotein receptors (ASGPRs)...
December 1, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29190243/factors-associated-with-serous-retinal-detachment-in-highly-myopic-eyes-with-vertical-oval-shaped-dome
#14
Antonio García-Ben, María J Morillo Sanchez, Ana Gonzalez Gómez, Ignacio García-Basterra, Antonio Soler García, Jose M García-Campos
PURPOSE: This study investigated factors associated with persistent serous retinal detachment in highly myopic eyes with vertical oval-shaped domes. METHODS: Twenty-eight highly myopic patients (40 eyes) with smooth macular elevations related to a vertical oval-shaped dome were recruited. Serous retinal detachment was investigated; 11 eyes had persistent submacular fluid (study group) and 29 eyes lacked submacular fluid (control group). All patients underwent complete ophthalmologic examinations, including axial-length measurement and fluorescein angiography...
November 22, 2017: Retina
https://www.readbyqxmd.com/read/29179705/vitrectomy-with-internal-limiting-membrane-peeling-versus-inverted-internal-limiting-membrane-flap-technique-for-macular-hole-induced-retinal-detachment-a-systematic-review-of-literature-and-meta-analysis
#15
Jing Yuan, Ling-Lin Zhang, Yu-Jie Lu, Meng-Yao Han, Ai-Hua Yu, Xiao-Jun Cai
BACKGROUND: To evaluate the effects on vitrectomy with internal limiting membrane (ILM) peeling versus vitrectomy with inverted internal limiting membrane flap technique for macular hole-induced retinal detachment (MHRD). METHODS: Pubmed, Cochrane Library, and Embase were systematically searched for studies that compared ILM peeling with inverted ILM flap technique for macular hole-induced retinal detachment. The primary outcomes are the rate of retinal reattachment and the rate of macular hole closure 6 months later after initial surgery, the secondary outcome is the postoperative best-corrected visual acuity (BCVA) 6 months later after initial surgery...
November 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29176526/macular-hole-formation-after-pars-plana-vitrectomy-for-primary-vitreoretinal-lymphoma
#16
Masatoshi Haruta, Chikako Taguchi, Ryoji Yamakawa
PURPOSE: To report a case of primary vitreoretinal lymphoma in which a macular hole developed after a diagnostic pars plana vitrectomy. METHODS: A retrospective interventional case report. RESULTS: A 65-year-old woman presented with worsening vision in the left eye. Fundus examination showed vitreous haze and multifocal, yellow-white infiltrates in the retina and under the retinal pigment epithelium in the left eye. She underwent a diagnostic pars plana vitrectomy in that eye...
November 22, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/29172443/membrane-crossing-and-membranotropic-activity-of-cell-penetrating-peptides-dangerous-liaisons
#17
Astrid Walrant, Sébastien Cardon, Fabienne Burlina, Sandrine Sagan
Living organisms have to maintain a stable balance in molecules and ions in the changing environment in which they are living, a process known as homeostasis. At the level of cells, the plasma membrane has a major role in homeostasis, since this hydrophobic film prevents passive diffusion of large and hydrophilic molecules between the extracellular and intracellular milieu. Living organisms have evolved with highly sophisticated transport systems to control exchanges across this barrier: import of nutrients and fuel essential for their survival; recognition of chemical or physical messengers allowing information interchanges with surrounding cells...
November 27, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29171223/-recent-advances-on-pericytes-in-microvascular-dysfunction-and-traditional-chinese-medicine-prevention
#18
REVIEW
Lei Liu, Jian-Xun Liu, Hao Guo, Jian-Xun Ren
Pericytesis a kind of widespread vascular mural cells embedded within the vascular basement membrane of blood microvessels, constituting the barrier of capillaries and tissue spaces together with endothelial cells. Pericytes communicate with microvascular endothelial cells through cell connections or paracrine signals, playing an important role in important physiological processes such as blood flow, vascular permeability and vascular formation. Pericytes dysfunction may participate in some microvascular dysfunction, and also mediate pathological repair process, therefore pericytes attracted more and more attention...
August 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29160787/functional-and-anatomical-significance-of-the-ectopic-inner-foveal-layers-in-eyes-with-idiopathic-epiretinal-membranes-surgical-results-at-12-months
#19
Andrea Govetto, Gianni Virgili, Francisco J Rodriguez, Marta S Figueroa, David Sarraf, Jean Pierre Hubschman
PURPOSE: To describe the functional and anatomical outcomes of pars plana vitrectomy with epiretinal membrane and internal limiting membrane peel in eyes with and without ectopic inner foveal layers (EIFLs). METHODS: In this retrospective multicenter study, patients diagnosed with idiopathic epiretinal membranes who underwent pars plana vitrectomy with epiretinal membrane and internal limiting membrane peel were enrolled, with a minimum follow-up of 12 months. Preoperative and postoperative spectral domain optical coherence tomography scans were qualitatively and quantitatively evaluated...
November 16, 2017: Retina
https://www.readbyqxmd.com/read/29160780/multiple-sub-internal-limiting-membrane-hemorrhages-with-double-ring-sign-in-eyes-with-valsalva-retinopathy
#20
Takeshi Iwase, Eimei Ra, Yasuki Ito, Hiroko Terasaki
No abstract text is available yet for this article.
November 16, 2017: Retina
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