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Internal limiting membrane

San-Ni Chen, Yi-Ting Hsieh, Chung-May Yang
PURPOSE: The aim of this paper was to evaluate the efficacy of multiple free internal limiting membrane (ILM) flap insertion in the management of macular hole-associated retinal detachment in high myopia. PROCEDURES: Eyes receiving operation for macular hole-associated retinal detachment were retrospectively recruited. Those in the study group received ILM peeling and multiple free ILM flap insertion, while those in the control group received ILM peeling only. Postoperative anatomical outcomes and best-corrected visual acuity were compared between the 2 groups...
June 6, 2018: Ophthalmologica. Journal International D'ophtalmologie
Zhongjing Lin, Yanwei Chen, Sha Gao, Yisheng Zhong, Xi Shen
BACKGROUND: To report a case series in which a modified technique was used to remove retained submacular perfluorocarbon liquid (PFCL) secondary to vitreoretinal surgery for rhegmatogenous retinal detachment. CASE PRESENTATION: Four patients who had undergone pars plana vitrectomy for rhegmatogenous retinal detachment were further treated with surgical intervention because of retained submacular PFCL. With a three-port pars plana approach, after the internal limiting membrane peeling with indocyanine green staining, a 38-gauge flexible cannula was used to aspirate the submacular perfluorocarbon bubble, followed by fluid-air exchange and air injection into vitreous cavity...
June 4, 2018: BMC Ophthalmology
Masanori Iwasaki, Takamasa Kinoshita, Hirotomo Miyamoto, Hiroko Imaizumi
PURPOSE: To examine the influence of inverted internal limiting membrane (ILM) flap technique on the outer retinal layer structures after macular hole (MH) surgery. METHODS: We included 24 eyes of 24 patients who underwent vitrectomy for large MHs (≥400 µm) with successful MH closure and observed for at least 6 months. Fourteen eyes were treated with inverted ILM flap technique (inverted group) and 10 with conventional ILM peeling (ILMP group). We evaluated the postoperative recovery rate of the external limiting membrane and ellipsoid zone and the best-corrected visual acuity...
May 8, 2018: Retina
Wen-Juan Lyu, Lei-Bing Ji, Yun Xiao, Yin-Bo Fan, Xue-Hong Cai
AIM: To investigate the effect of internal limiting membrane transplantation and autologous blood on treating refractory giant macular hole. METHODS: Thirty-seven eyes with giant macular hole of the smallest hole diameter >700 µm, the maximum diameter of the substrate >1000 µm and hole formation factor <0.6 underwent surgical treatment. The patients were randomly divided into two groups. Nineteen eyes with surgical flip of the internal limiting membrane in group A, 18 eyes with internal limiting membrane transplantation in group B who underwent the tamponade of internal limiting membrane into the hole, autologous plasma was used to seal the hole...
2018: International Journal of Ophthalmology
Chen Jiang, Lu Ruan, Juan Zhang, Xin Huang
PURPOSE: To study retinal neovascularization (RNV) inhibition by intravitreal injections (IV) of ranibizumab, sTie2 fusion protein (sTie2-Fc), and a combined therapy in an oxygen-induced retinopathy (OIR) model. MATERIALS AND METHODS: An OIR mouse model was used to simulate RNV in retinopathy of prematurity (ROP); and the effect of blocking the angiopoietin (Ang) and its receptor (Tie2) and the vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling pathways was compared using an IV of sTie2-Fc (Ang inhibitor) and/or ranibizumab (aVEGF antagonist)...
June 1, 2018: Current Eye Research
Kelvin H Wan, Alexander K N Lam, Christopher Kai-Shun Leung
Importance: Whether optical coherence tomography angiography (OCT-A) outperforms OCT to detect glaucoma remains inconclusive. Objective: To compare (1) the diagnostic performance for detection of glaucoma and (2) the structure-function association between inner macular vessel density and inner macular thickness. Design, Setting, and Participants: This cross-sectional study included 115 patients with glaucoma and 35 healthy individuals for measurements of retinal thickness and retinal vessel density, segmented between the anterior boundary of internal limiting membrane and the posterior boundary of the inner plexiform layer, over the 3 × 3-mm2 macula using swept-source OCT...
May 31, 2018: JAMA Ophthalmology
Fang-Yi Su, Jasmin Chen, Hye-Nam Son, Abby M Kelly, Anthony J Convertine, T Eoin West, Shawn J Skerrett, Daniel M Ratner, Patrick S Stayton
Pulmonary intracellular infections, such as tuberculosis, anthrax, and tularemia, have remained a significant challenge to conventional antibiotic therapy. Ineffective antibiotic treatment of these infections can lead not only to undesired side effects, but also to the emergence of antibiotic resistance. Aminoglycosides (e.g., streptomycin) have long been part of the therapeutic regiment for many pulmonary intracellular infections. Their bioavailability for intracellular bacterial pools, however, is limited by poor membrane permeability and rapid elimination...
May 31, 2018: Biomaterials Science
Joanna Stafiej, Karolina Kaźmierczak, Katarzyna Linkowska, Paweł Żuchowski, Tomasz Grzybowski, Grażyna Malukiewicz
Purpose: To evaluate the expression profiles of the VEGF α and TGF β in the ERMs and ILMs in retinal disorders. Methods: In this nonrandomized prospective study, 75 patients (34 females and 41 males) referred to pars plana vitrectomy (PPV) due to different retinal diseases were enrolled to the study. The samples of ERMs and ILMs collected during PPV were immediately put in TRIzol® Reagent (Life Technologies, USA) and stored at -70°C until RNA extraction. Gene expression analysis was done with TaqMan® Gene Expression Assays (Applied Biosystems, USA) following the manufacturer's instructions...
2018: Journal of Ophthalmology
Peng-Peng Zhao, Shuang Wang, Nan Liu, Zhi-Min Shu, Jin-Song Zhao
The surgical outcomes of macular holes (MHs) have improved greatly in recent years. The closure rate is as high as 90-100%, but the outcomes of some special types of MHs remain unsatisfactory. Internal limiting membrane (ILM) peeling dramatically improves the anatomic success rate, but recent studies have found that it could also cause mechanical and subclinical traumatic changes to the retina. Dyes are widely used, and apart from indocyanine green (ICG), the toxicities of other dyes require further research...
2018: Journal of Ophthalmology
Zizhong Hu, Xiaojian Ye, Xuehua Lv, Kang Liang, Weiwei Zhang, Xi Chen, Erbing Cao, Xunyi Gu, Qinghuai Liu, Ping Xie
PURPOSE: The purpose of this study is to investigate the effectiveness of a new surgical technique of non-inverted pedicle internal limiting membrane (ILM) transposition for the treatment of eyes with large macular hole. METHODS: This is a retrospective, consecutive, interventional case series. Twelve eyes of 12 consecutive patients who underwent vitrectomy for the treatment of a large macular hole (MH size > 400 µm) were treated. ILM was peeled and left with a pedicle attached to the superior temporal retina...
May 29, 2018: Eye
Mopei Wang, Li Liang, Xiudong Lei, Asha Multani, Funda Meric-Bernstam, Debasish Tripathy, Yun Wu, Hui Chen, Hong Zhang
PURPOSE: To evaluate the incidence of cMET proto-oncogene aberration in a cohort of triple negative breast cancers using immunohistochemistry and fluorescence in situ hybridization (FISH) methods and correlated with patient outcome. PATIENTS AND METHODS: One hundred and six female patients with diagnosis of triple negative invasive breast carcinoma at The University of Texas-M D Anderson Cancer Center from 1983 to 2009 were included in the study. Expression of cMET was assessed by IHC using rabbit monoclonal anti-total cMET antibody (SP44 from Ventana)...
May 3, 2018: Annals of Diagnostic Pathology
Keiichi Inoue, Shinya Tahara, Yoshitaka Kato, Satoshi Takeuchi, Tahei Tahara, Hideki Kandori
Parvularcula oceani xenorhodopsin (PoXeR) is the first light-driven inward proton pump. Although the heptahelical transmembrane structure binding a chromophore retinal is similar to those of typical outward proton pumps, the direction of transport is strictly regulated to be opposed. To understand the mechanism of inward proton transport, comprehensive transient absorption spectroscopy was conducted. Ultrafast pump-probe spectroscopy revealed that the isomerization time of retinal is 1.2 ps, which is considerably slower than those of other microbial rhodopsins (180-770 fs)...
May 29, 2018: Journal of Physical Chemistry. B
Takuhiro Otosu, Shoichi Yamaguchi
Fluorescence lifetime correlation analysis is becoming a powerful tool to understand the conformational heterogeneity of biomolecules and their dynamics with an unprecedented detection sensitivity and time resolution. However, its application to the study of biomembranes is very limited. Here, we report on two-dimensional fluorescence lifetime correlation spectroscopy (2D FLCS) in combination with total internal reflection (TIR) microscopy (TIR 2D-FLCS). High depth resolution in TIR microscopy and species-specific correlation analysis in 2D FLCS give us the opportunity to selectively analyze molecules in or on a supported lipid bilayer, a model biomembrane formed on the glass surface...
May 25, 2018: Journal of Physical Chemistry. B
Xusheng Qiu, Yixin Chen, Xiaoyang Qi, Zhipeng Yin, Yan Zhang, Zhen Wang
Objective: To evaluate the effectiveness of induced membrane technique in the treatment of infectious bone defect. Methods: Thirty-six patients (37 bone lesions) with infectious bone defects were treated with induced membrane technique between January 2011 and June 2014. There were 28 males and 8 females with an average age of 36 years (range, 20-68 years). All bone defects were post-traumatic infectious bone defect. The bone defect was located at the tibia and fibula in 24 cases (25 bone lesions), at femurs in 6 cases (6 bone lesions), at ulnas and radii in 2 cases (2 bone lesions), at calcanei in 3 cases (3 bone lesions), and at clavicle in 1 case (1 bone lesion)...
September 1, 2017: Chinese Journal of Reparative and Reconstructive Surgery
Emily E Bosco, R James Christie, Rosa Carrasco, Darrin Sabol, Jiping Zha, Karma DaCosta, Lee Brown, Maureen Kennedy, John Meekin, Sandrina Phipps, Joanne Ayriss, Qun Du, Binyam Bezabeh, Partha Chowdhury, Shannon Breen, Cui Chen, Molly Reed, MaryJane Hinrichs, Haihong Zhong, Zhan Xiao, Rakesh Dixit, Ronald Herbst, David A Tice
Despite recent advances in treatment, breast cancer remains the second-most common cause of cancer death among American women. A greater understanding of the molecular characteristics of breast tumors could ultimately lead to improved tumor-targeted treatment options, particularly for subsets of breast cancer patients with unmet needs. Using an unbiased genomics approach to uncover membrane-localized tumor-associated antigens (TAAs), we have identified glial cell line derived neurotrophic factor (GDNF) family receptor α 1 (GFRA1) as a breast cancer TAA...
May 1, 2018: Oncotarget
Gakushi Tsuji, Takeshi Sunami, Norikazu Ichihashi
Giant unilamellar vesicles (GUVs) are large vesicles bounded by a single lipid bilayer, which have been used in various applications as artificial, cell-like compartments. The water-in-oil (w/o) emulsion-transfer method has been attracting attention as a method to prepare GUVs that can efficiently encapsulate macromolecules. For efficient GUV production by this method, non-physiological, high concentrations of sugars are usually required in the inner solution of the GUVs. These sugars limit the utility of the GUVs for a wide range of applications...
May 21, 2018: Journal of Bioscience and Bioengineering
Chun Ding, Shengguo Li, Jun Zeng
PURPOSE: The aim of this study was to demonstrate the surgical technique and clinical outcome of autologous neurosensory retinal patch transplantation for recurrent large macular hole (MH)-induced retinal detachment after failed surgery with internal limiting membrane (ILM) removal or transplantation. METHODS: We reviewed 5 patients with recurrent MH-induced retinal detachment after failed surgeries with ILM removal or transplantation who underwent vitrectomy combined with autologous neurosensory retinal patch transplantations and were followed up over 6 months...
May 22, 2018: Ophthalmic Research
Chun-Yu Lai, Pan-Long Lv, Qiu-Yi Dong, Shi Lei Yeo, Bruce E Rittmann, He-Ping Zhao
This work demonstrates bromate (BrO3-) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3-) on BrO3- reduction, as well as formation of poly--hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3- enhanced BrO3- reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3- and NO3- simultaneously...
May 22, 2018: Environmental Science & Technology
Marco Coassin, Valentina Mastrofilippo, Jay M Stewart, Andrea Fanti, Matteo Belpoliti, Luca Cimino, Alfonso Iovieno, Luigi Fontana
PURPOSE: To determine long-term results of vitrectomy for lamellar macular holes (LMH). To evaluate how the type of lamellar macular hole (tractional vs. degenerative) and the crystalline lens status might influence visual outcomes. METHODS: We collected data from 106 patients with symptomatic lamellar macular hole that underwent pars plana vitrectomy with membranectomy and internal limiting membrane peeling. Best-corrected visual acuity (BCVA) and optical coherence tomography appearance were determined preoperatively and postoperatively...
May 21, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Kevin Sugier, Benoit Vacherie, Astrid Cornils, Patrick Wincker, Jean-Louis Jamet, Mohammed-Amin Madoui
Among copepods, which are the most abundant animals on Earth, the genus Oithona is described as one of the most numerous and plays a major role in the marine food chain and biogeochemical cycles, particularly through the excretion of chitin-coated fecal pellets. Despite the morphology of several Oithona species is well known, knowledge of its internal anatomy and chitin distribution is still limited. To answer this problem, Oithona nana and O. similis individuals were stained by Wheat Germ Agglutinin-Fluorescein IsoThioCyanate (WGA-FITC) and DiAmidino-2-PhenylIndole (DAPI) for fluorescence microscopy observations...
2018: PeerJ
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