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Internal limiting membrane

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https://www.readbyqxmd.com/read/28637277/measuring-co2-and-hco3-permeabilities-of-isolated-chloroplasts-using-a-mims-18o-approach
#1
Dimitri Tolleter, Vincent Chochois, Richard Poiré, G Dean Price, Murray R Badger
To support photosynthetic CO2 fixation by Rubisco, the chloroplast must be fed with inorganic carbon in the form of CO2 or bicarbonate. However, the mechanisms allowing the rapid passage of this gas and this charged molecule through the bounding membranes of the chloroplast envelope are not yet completely elucidated. We describe here a method allowing us to measure the permeability of these two molecules through the chloroplast envelope using a membrane inlet mass spectrometer and 18O-labelled inorganic carbon...
June 16, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28631180/microvascular-changes-after-vitrectomy-with-internal-limiting-membrane-peeling-an-optical-coherence-tomography-angiography-study
#2
Leonardo Mastropasqua, Enrico Borrelli, Paolo Carpineto, Lisa Toto, Luca Di Antonio, Peter A Mattei, Rodolfo Mastropasqua
PURPOSE: To evaluate superficial capillary plexus (SCP) changes occurring after internal limiting membrane (ILM) peeling for the treatment of idiopathic epiretinal membrane (ERM). METHODS: A total of 15 eyes of 15 patients affected by idiopathic ERM (eight males and seven females; mean age 59.8 ± 9.6 years) were enrolled in the study. Patients were treated with pars plana vitrectomy followed by ERM and ILM peeling. Subjects were evaluated at baseline and at the week-1 and month-1 follow-up visits...
June 19, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28630788/evaluation-of-ganglion-cell-inner-plexiform-layer-thickness-after-vitreoretinal-surgery-with-internal-limiting-membrane-peeling-in-cases-with-idiopathic-macular-hole
#3
Sibel Demirel, Ahmed Abdullayev, Özge Yanık, Figen Batıoğlu, Emin Özmert
OBJECTIVES: To evaluate macular retinal ganglion cell-inner plexiform layer (GCIPL) thickness after vitrectomy with internal limiting membrane (ILM) peeling for idiopathic macular holes using spectral domain optical coherence tomography (SD-OCT). MATERIALS AND METHODS: Eighteen eyes of 18 patients with unilateral idiopathic macular hole who underwent vitrectomy with ILM peeling were retrospectively analyzed. Healthy fellow eyes of the patients and 18 eyes of 18 age-matched healthy individuals constituted the control group...
June 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/28622372/vitrectomy-with-or-without-internal-limiting-membrane-peeling-for-idiopathic-epiretinal-membrane-a-meta-analysis
#4
Wei-Cheng Chang, Chin Lin, Cho-Hao Lee, Tzu-Ling Sung, Tao-Hsin Tung, Jorn-Hon Liu
BACKGROUND: Studies on vitrectomy with and without internal limiting membrane (ILM) peeling for idiopathic epiretinal membrane (ERM) have yielded uncertain results regarding clinical outcomes and recurrence rates. OBJECTIVE: To compare the clinical outcomes of vitrectomy with and without ILM peeling for idiopathic ERM. METHODS: Databases, including PubMed, Embase, Cochrane, Web of Science, Google Scholar, CNKI databases, FDA.gov, and ClinicalTrials...
2017: PloS One
https://www.readbyqxmd.com/read/28615286/the-physiology-of-phagocytosis-in-the-context-of-mitochondrial-origin
#5
REVIEW
William F Martin, Aloysius G M Tielens, Marek Mentel, Sriram G Garg, Sven B Gould
How mitochondria came to reside within the cytosol of their host has been debated for 50 years. Though current data indicate that the last eukaryote common ancestor possessed mitochondria and was a complex cell, whether mitochondria or complexity came first in eukaryotic evolution is still discussed. In autogenous models (complexity first), the origin of phagocytosis poses the limiting step at eukaryote origin, with mitochondria coming late as an undigested growth substrate. In symbiosis-based models (mitochondria first), the host was an archaeon, and the origin of mitochondria was the limiting step at eukaryote origin, with mitochondria providing bacterial genes, ATP synthesis on internalized bioenergetic membranes, and mitochondrion-derived vesicles as the seed of the eukaryote endomembrane system...
September 2017: Microbiology and Molecular Biology Reviews: MMBR
https://www.readbyqxmd.com/read/28613224/cutting-the-internal-limiting-membrane-with-zero-aspiration-technique-a-clinical-audit
#6
Rumana N Hussain, David H W Steel, Teresa Sandinha, Theodor Stappler, Heinrich Heimann, David Wong
No abstract text is available yet for this article.
April 14, 2017: Retina
https://www.readbyqxmd.com/read/28611062/selective-imaging-of-internalized-proteopathic-%C3%AE-synuclein-seeds-in-primary-neurons-reveals-mechanistic-insight-into-transmission-of-synucleinopathies
#7
Richard J Karpowicz, Conor M Haney, Tiberiu S Mihaila, Raizel M Sandler, E James Petersson, Virginia M-Y Lee
Direct cell-to-cell transmission of proteopathic alpha-synuclein (α-syn) aggregates is thought to underlie the progression of neurodegenerative synucleinopathies. However, the specific intracellular processes governing this transmission remain unclear because currently-available model systems are limited. For example, in cell culture models of α-syn-seeded aggregation, it is difficult to discern intracellular from extracellular exogenously applied α-syn seed species. Herein, we employed fluorescently labeled α-syn preformed fibrils (pffs) in conjunction with the membrane-impermeable fluorescence quencher trypan blue to selectively image internalized α-syn seeds in cultured primary neurons and to quantitatively characterize the concentration dependence, time course, and inhibition of pff uptake...
June 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28609625/conformational-dynamics-of-the-lipopolysaccharide-from-escherichia-coli-o91-revealed-by-nmr-spectroscopy-and-molecular-simulations
#8
Pilar Blasco, Dhilon S Patel, Olof Engström, Wonpil Im, Göran Widmalm
The outer leaflet of the outer membrane in gram-negative bacteria contains lipopolysaccharides (LPS) as a major component, and the outer membrane provides a physical barrier and protection against hostile environments. The enterohemorrhagic E. coli of serogroup O91 has an O-antigen polysaccharide (PS) with five sugar residues in the repeating unit (RU) and the herein studied O-antigen PS contains ~10 RUs. 1H,13C-HSQC-NOESY experiments on a [1-13C]-labeled PS were employed to deduce 1H,1H cross-relaxation rates and transglycosidic 3JCH related to the  torsional angles...
June 13, 2017: Biochemistry
https://www.readbyqxmd.com/read/28606265/-the-fundus-manifestations-and-sd-oct-findings-of-patients-with-acute-vogt-koyanagi-harada-disease
#9
B Li, J J Ye, M F Zhang, D H Li
Objective: To conclude the characteristics of fundus appearance and spectral domain optical coherence tomography(SD-OCT) findings of patients with acute Vogt-Koyanagi-Harada (VKH) disease. Methods: The clinical data of 17 patients (34 eyes) diagnosed of acute VKH in Peking Union Medical College Hospital from Jan. 2012 to Dec. 2014 were studied retrospectively.Examinations included visual acuity, slit lamp, fundus, color fundus pictures, FFA and SD-OCT. Results: Eight men and 9 women were enrolled with mean age of (40...
June 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28604981/optic-disc-pit-maculopathy-after-prone-positioning-spine-surgery
#10
Marcos Ruiz-Sánchez, Maria E Rodríguez-González-Herrero, Jenifer Ruiz-Sará
PURPOSE: To report a case of optic disc pit maculopathy after spine surgery performed in the prone position. METHODS: Case report. RESULTS: A 32-year-old man underwent spine surgery for disc herniation repair. He complained of visual acuity loss immediately after the procedure. Optic disc maculopathy with internal limiting membrane detachment was found to be the cause. Vitrectomy was scheduled after a 12-month follow-up without resolution of the maculopathy...
June 9, 2017: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28602209/update-on-glut4-vesicle-traffic-a-cornerstone-of-insulin-action
#11
REVIEW
Javier R Jaldin-Fincati, Martin Pavarotti, Scott Frendo-Cumbo, Philip J Bilan, Amira Klip
Glucose transport is rate limiting for dietary glucose utilization by muscle and fat. The glucose transporter GLUT4 is dynamically sorted and retained intracellularly and redistributes to the plasma membrane (PM) by insulin-regulated vesicular traffic, or 'GLUT4 translocation'. Here we emphasize recent findings in GLUT4 translocation research. The application of total internal reflection fluorescence microscopy (TIRFM) has increased our understanding of insulin-regulated events beneath the PM, such as vesicle tethering and membrane fusion...
June 8, 2017: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/28591131/visualization-of-early-influenza-a-virus-trafficking-in-human-dendritic-cells-using-sted-microscopy
#12
Faezzah Baharom, Oliver S Thomas, Rico Lepzien, Ira Mellman, Cécile Chalouni, Anna Smed-Sörensen
Influenza A viruses (IAV) primarily target respiratory epithelial cells, but can also replicate in immune cells, including human dendritic cells (DCs). Super-resolution microscopy provides a novel method of visualizing viral trafficking by overcoming the resolution limit imposed by conventional light microscopy, without the laborious sample preparation of electron microscopy. Using three-color Stimulated Emission Depletion (STED) microscopy, we visualized input IAV nucleoprotein (NP), early and late endosomal compartments (EEA1 and LAMP1 respectively), and HLA-DR (DC membrane/cytosol) by immunofluorescence in human DCs...
2017: PloS One
https://www.readbyqxmd.com/read/28590966/characteristics-of-epiretinal-membrane-remnant-edge-by-optical-coherence-tomography-after-pars-plana-vitrectomy
#13
Raouf Gaber, Qi Sheng You, Ilkay Kilic Muftuoglu, Mostafa Alam, Frank F Tsai, Nadia Mendoza, William R Freeman
PURPOSE: To evaluate the incidence, characteristics, and the progression of epiretinal membrane (ERM) remnant edge seen by optical coherence tomography after ERM peeling. METHODS: A retrospective chart review was conducted for 86 eyes of 85 consecutive patients who were diagnosed with ERM and underwent pars plana vitrectomy for epiretinal membrane peeling between 2013 and 2014. Data collected and analyzed included age, gender, preoperative and postoperative visual acuity, use of indocyanine green dye to stain internal limiting membrane, tamponade used after vitrectomy, ERM edge boundaries, presence of cystoid macular edema, and central foveal thickness...
January 11, 2017: Retina
https://www.readbyqxmd.com/read/28590320/sticky-silicone-oil
#14
Hamouda Hamdi Ghoraba, Adel Galal Zaky, Haithem Mamoun Abd Al Fatah, Emad El Din Mohamed El Gemai, Mohamed Amin Heikal
OBJECTIVE: To report and analyze the phenomenon of sticky silicone oil on the retina at the time of its removal. BACKGROUND: Silicone oil has been used for more than 25 years as a long-term retinal tamponade in the surgical management of patients with complex retinal detachments. When a decision to remove silicone oil was made at the end of the tamponade period, the system involved a number of different materials: silicone oil, aqueous solution with or without remnants of perfluorocarbon liquid plus the material of the cannula used to aspirate Silicone oil...
October 31, 2016: Retina
https://www.readbyqxmd.com/read/28581000/shedding-light-on-zwitterionic-magnetic-nanoparticles-limitations-for-in-vivo-applications
#15
Manuel Pernia Leal, Carlos Caro, María Luisa García-Martín
Over the last few years several studies have dealt with the importance of the surface charge of nanoparticles in prolonging their blood circulation and minimizing their interaction with plasma proteins. These investigations claimed that zwitterionic nanoparticles exhibited a minimal macrophage response and long blood circulation times compared to nanoparticles with other surface charges. These differences in their in vivo behavior are mainly attributed to the interaction of nanoparticles with plasma proteins...
June 5, 2017: Nanoscale
https://www.readbyqxmd.com/read/28574004/surgical-management-of-optic-disc-pit-maculopathy-with-a-fovea-sparing-internal-limiting-membrane-flap
#16
Rupak Roy, Kumar Saurabh, Nicey Roy Thomas, Kalpita Das
Optic disc pit (ODP) is rare congenital cavitary anomaly of the optic disc. Serous detachment of macula is the most common complication of ODP and occurs in 25%-75% of these cases. Although various surgical techniques have been used for the treatment of ODP maculopathy; consensus still eludes as far as the optimal surgical approach is concerned. We herein report a case of ODP maculopathy in a young female treated successfully with vitrectomy, fovea sparing internal limiting membrane flap, and C3F8 tamponade...
May 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28570483/the-efficacy-of-superior-inverted-internal-limiting-membrane-flap-technique-for-the-treatment-of-full-thickness-macular-hole
#17
Se Rang Choi, Joon Won Kang, Ji Hoon Jeon, Joo Young Shin, Bum-Joo Cho, Baek-Lok Oh, Jang Won Heo
No abstract text is available yet for this article.
May 29, 2017: Retina
https://www.readbyqxmd.com/read/28567164/pars-plana-vitrectomy-with-internal-limiting-membrane-peeling-for-nontractional-diabetic-macular-edema
#18
Jan Niklas Ulrich
BACKGROUND: Diabetes mellitus remains the leading cause of blindness among working age Americans with diabetic macular edema being the most common cause for moderate and severe vision loss. OBJECTIVE: To investigate the anatomical and visual benefits of pars plana vitrectomy with inner limiting membrane peeling in patients with nontractional diabetic macular edema as well as correlation of integrity of outer retinal layers on spectral domain optical coherence tomography to visual outcomes...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28566574/quantitative-targeted-absolute-proteomics-qtap-based-pharmacoproteomics-the-importance-of-international-collaboration
#19
Tetsuya Terasaki
 Proteins such as membrane transporters, enzymes, receptors and channels play key roles in drug absorption, distribution, metabolism, and elimination, and also influence efficacy and the likelihood of adverse reactions. Therefore, if we can quantify the activities of these molecules, it may be possible to predict the behavior of candidate drugs in humans in disease states; such methodology would be extremely helpful for efficient drug development. We have developed an in silico method to select appropriate peptides within amino acid sequences in order to quantify targeted proteins by LC-MS/MS in selected reaction monitoring (SRM) mode...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28559838/ten-year-follow-up-after-bilateral-submacular-neovascular-membrane-removal-in-a-case-of-autosomal-recessive-bestrophinopathy
#20
Carlos A Moreira, Carlos A Moreira-Neto, Mario Junqueira Nobrega, Eduardo Cunha de Souza
Herein, we report the case of an 8-year-old girl who presented in December 2000 with a submacular neovascular membrane in the right eye, with a clinical diagnosis of Best disease. At that time, she underwent pars plana vitrectomy (PPV) with removal of the subretinal choroidal neovascularization (CNV). Her vision improved from 20/200 to 20/25. Four years later, a new CNV developed in the other eye. Initially, she underwent unsuccessful photodynamic therapy. As her vision worsened, she underwent a second, this time successful, PPV with membrane removal in the left eye, with vision improving to 20/30...
January 2017: Case Reports in Ophthalmology
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