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https://www.readbyqxmd.com/read/29325125/age-phosphorus-and-25-hydroxycholecalciferol-regulate-mrna-expression-of-vitamin-d-receptor-and-sodium-phosphate-cotransporter-in-the-small-intestine-of-broiler-chickens
#1
J C Han, J L Zhang, N Zhang, X Yang, H X Qu, Y Guo, C X Shi, Y F Yan
Four experiments were conducted in this study. Experiment 1 was carried out to examine mRNA expressions of nuclear vitamin D receptor (nVDR), membrane vitamin D receptor (mVDR), and type IIb sodium-phosphate cotransporter (NaPi-IIb) in the small intestine of broiler chickens. Experiments 2, 3, and 4 were implemented to evaluate effects of age, non-phytate phosphorus (NPP), and 25-hydroxycholecalciferol (25-OH-D3) on mRNA expressions of nVDR, mVDR, and NaPi-IIb in the duodenum of chickens. Results showed that mRNA expression levels of nVDR and NaPi-IIb were highest in the duodenum of 21-day-old broilers, lower in the jejunum, and lowest in the ileum...
January 6, 2018: Poultry Science
https://www.readbyqxmd.com/read/29317227/prolonged-exposure-to-1-25-oh-2d3-and-high-ionized-calcium-induces-fgf-23-production-in-intestinal-epithelium-like-caco-2-monolayer-a-local-negative-feedback-for-preventing-excessive-calcium-transport
#2
Mayuree Rodrat, Kannikar Wongdee, Natthaphon Panupintu, Jirawan Thongbunchoo, Jarinthorn Teerapornpuntakit, Nateetip Krishnamra, Narattaphol Charoenphandhu
Overdose of oral calcium supplement and excessive intestinal calcium absorption can contribute pathophysiological conditions, e.g., nephrolithiasis, vascular calcification, dementia, and cardiovascular accident. Since our previous investigation has indicated that fibroblast growth factor (FGF)-23 could abolish the 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-enhanced calcium absorption, we further hypothesized that FGF-23 produced locally in the enterocytes might be part of a local negative feedback loop to regulate calcium absorption...
January 6, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29316495/vdr-and-gc-gene-polymorphisms-modulate-the-risk-of-lumbar-disc-degeneration-in-iran
#3
Sohail Mashayekhi, Alia Saberi, Zivar Salehi, Gelareh Biazar, Roghayeh Mehrdel
OBJECTIVE: Lumbar disc degeneration (LDD) occurs commonly in humans. Vitamin D metabolic and signaling pathway plays a significant role in intervertebral disc degeneration. The aim of this study was to evaluate the influence of the genetic polymorphism in the two key genes of 1,25-(OH)2-D3 pathway, VDR (vitamin D receptor) and GC (group-specific component), in LDD development. PATIENTS AND METHODS: Two single-nucleotide polymorphisms, VDR rs2228570 (FokI) and GC rs7041, were genotyped in 180 patients with LDD and 230 healthy individuals...
December 29, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29315269/mining-the-potential-of-label-free-biosensors-for-in-vitro-antipsychotic-drug-screening
#4
Tugba Kilic, Maria Soler, Nafiseh Fahimi-Kashani, Hatice Altug, Sandro Carrara
The pharmaceutical industry is facing enormous challenges due to high drug attribution rates. For the past decades, novel methods have been developed for safety and efficacy testing, as well as for improving early development stages. In vitro screening methods for drug-receptor binding are considered to be good alternatives for decreasing costs in the identification of drug candidates. However, these methods require lengthy and troublesome labeling steps. Biosensors hold great promise due to the fact that label-free detection schemes can be designed in an easy and low-cost manner...
January 9, 2018: Biosensors
https://www.readbyqxmd.com/read/29313985/cart-peptide-in-the-nucleus-accumbens-shell-inhibits-cocaine-induced-locomotor-sensitization-to-transient-overexpression-of-%C3%AE-ca2-calmodulin-dependent-protein-kinase-ii
#5
Lixia Xiong, Qing Meng, Xi Sun, Xiangtong Lu, Qiang Fu, Qinghua Peng, Jianhua Yang, Ki-Wan Oh, Zhen Zhen Hu
Cocaine- and amphetamine-regulated transcript (CART) peptide is a widely distributed neurotransmitter that attenuates cocaine-induced locomotor activity when injected into the nucleus accumbens (NAc). Our previous work first confirmed that the inhibitory mechanism of the CART peptide on cocaine-induced locomotor activity is related to a reduction in cocaine-enhanced phosphorylated Ca2+ /calmodulin-dependent protein kinaseIIα (pCaMKIIα) and the enhancement of cocaine-induced D3R function. The present study investigated whether CART peptide inhibited cocaine-induced locomotor activity via inhibition of interactions between pCaMKIIα and the D3 dopamine receptor (D3R)...
January 4, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29301117/cooperative-effects-of-q10-vitamin-d3-and-l-arginine-on-cardiac-and-endothelial-cells
#6
Claudio Molinari, Vera Morsanuto, Simona Polli, Francesca Uberti
This work demonstrates the cooperative effect of Q10, vitamin D3, and L-arginine on both cardiac and endothelial cells. The effects of Q10, L-arginine, and vitamin D3 alone or combined on cell viability, nitric oxide, and reactive oxygen species productions in endothelial and cardiac cells were studied. Moreover, the involvement of PI3K/Akt and ERK/MAPK pathways leading to eNOS activation as well as the involvement of vitamin D receptor were also investigated. The same agents were tested in an animal model to verify vasodilation, nitric oxide, and reactive oxygen species production...
January 5, 2018: Journal of Vascular Research
https://www.readbyqxmd.com/read/29298375/single-molecule-force-spectroscopy-reveals-two-domain-binding-mode-of-pilus-1-tip-protein-rrga-of-streptococcus-pneumoniae-to-fibronectin
#7
Tanja D Becke, Stefan Ness, Raimund Gürster, Arndt F Schilling, Anne-Marie di Guilmi, Stefanie Sudhop, Markus Hilleringmann, Hauke Clausen-Schaumann
For host cell adhesion and invasion, surface piliation procures benefits for bacteria. A detailed investigation of how pili adhere to host cells is therefore a key aspect in understanding their role during infection. Streptococcus pneumoniae TIGR 4, a clinical relevant serotype 4 strain, is capable of expressing pilus-1 with terminal RrgA, an adhesin interacting with host extracellular matrix (ECM) proteins. We used single molecule force spectroscopy to investigate the binding of full length RrgA and single RrgA domains to fibronectin...
January 3, 2018: ACS Nano
https://www.readbyqxmd.com/read/29290431/targeting-antimicrobial-defenses-of-the-udder-through-an-intrinsic-cellular-pathway
#8
Corwin D Nelson, Kathryn E Merriman, Michael B Poindexter, Mercedes F Kweh, Leslie P Blakely
The bovine innate immune system has a strong repertoire of antimicrobial defenses to rapidly attack infectious pathogens that evade physical barriers of the udder. Exploration of the intracrine vitamin D pathway of bovine macrophages has improved understanding of the signals that initiate antimicrobial defenses that protect the udder. In the intracrine vitamin D pathway, pathogen recognition receptors upregulate CYP27B1 mRNA that encodes for the enzyme that converts 25-hydroxyvitamin D [25(OH)D3] to the active vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]...
December 28, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29289835/antinociceptive-anti-inflammatory-and-toxicological-evaluation-of-semi-synthetic-molecules-obtained-from-a-benzyl-isothiocyanate-isolated-from-moringa-oleifera-lam-in-a-temporomandibular-joint-inflammatory-hypernociception-model-in-rats
#9
Alain Oliveira Dos Santos, Danielle Rocha do Val, Felipe Dantas da Silveira, Francisco Isaac Fernandes Gomes, Hermany Capistrano Freitas, Ellen Lima de Assis, Diana Kelly Castro de Almeida, Igor Iuco Castro da Silva, Francisco Geraldo Barbosa, Jair Mafezoli, Marcos Reinaldo da Silva, Gerly Anne de Castro Brito, Juliana Trindade Clemente-Napimoga, Vicente de Paulo Teixeira de Paulo Teixera Pinto, Gerardo Cristino Filho, Mirna Marques Bezerra, Hellíada Vasconcelos Chaves
Inflammation is a key component of many clinical conditions that affect the temporomandibular joint (TMJ) and Moringa oleifera Lam. has been used to treat inflammatory diseases. Here, we evaluated the toxicological effects on mice of a naturally-occurring isothiocyanate from M. oleifera and its seven analogue molecules. Further, the anti-nociceptive and anti-inflammatory effects on a rat model of TMJ inflammatory hypernociception were assessed. The systemic toxicological profile was determined in mice over a 14-day period: MC-1 1 μg/kg; MC-D1 1 μg/kg, MC-D3 100 μg/kg, MC-D6 1 μg/kg, MC-D7 1 μg/kg, MC-D8 1 μg/kg, MC-D9 10 μg/kg, and MC-H 1 μg/kg...
December 28, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29287957/novel-vdr-antagonists-based-on-the-gw0742-scaffold
#10
Kelly A Teske, Jonathan W Bogart, Leggy A Arnold
The vitamin D receptor is a nuclear hormone receptor that regulates cell proliferation, cell differentiation and calcium homeostasis. The receptor is endogenously activated by 1,25-dihydroxyvitamin D3, which induces transcription of VDR targets genes regulated by coactivator binding. VDR antagonists and partial agonists have been developed based on the secosteroid scaffold of vitamin D. Only a few non-secosteroid VDR antagonists are known. Herein, we report the rational design of non-secosteroid VDR antagonists using GW0742 as a scaffold...
December 19, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29287794/effect-of-glucocorticoid-treatment-on-baff-and-april-expression-in-patients-with-immune-thrombocytopenia-itp
#11
Julian Kamhieh-Milz, Nuha Ghosoun, Viktor Sterzer, Abdulgabar Salama
Immune thrombocytopenic purpura (ITP) is an idiopathic bleeding disorder. B cell activating factor (BAFF) and 'A proliferation-inducing ligand' (APRIL) have regulatory effects on B and T cells and may represent relevant factors in the pathogenesis of ITP. Serum levels and gene expression were investigated in ITP patients. Both BAFF and APRIL serum levels were significantly elevated in active ITP. However, gene expression analysis revealed both factors to have a tendency toward downregulation. Glucocorticoid treatment significantly reduced BAFF but not APRIL serum levels, which may be mediated by differences in transcription factor binding sites...
December 26, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29285101/proteomic-changes-of-cd4-cd25-forkhead-box-p3-regulatory-t-cells-in-a-30-day-rat-model-of-sepsis-survival
#12
Yuxia Jiao, Siqi Tan, Junyu Xiong
Sepsis is defined as life threatening organ dysfunction arising from a dysregulated host response to infection. The outcomes of sepsis include early mortality, delayed mortality and recovery, and depend on the inflammatory response. Previous studies have demonstrated that regulatory T cells (Tregs) are important in determining the outcome of sepsis, as their suppressive function serves a role in maintaining immune homeostasis. However, Treg-mediated immunosuppression during the course of sepsis remains unclear and little is known about the survival of patients following diagnosis...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29281262/binding-of-zn-ii-to-tropomyosin-receptor-kinase-a-in-complex-with-its-cognate-nerve-growth-factor-insights-from-molecular-simulation-and-in-vitro-essays
#13
Adriana Pietropaolo, Antonio Magrì, Valentina Greco, Valeria Losasso, Diego La Mendola, Sebastiano Sciuto, Paolo Carloni, Enrico Rizzarelli
The binding of the human nerve growth factor (NGF) protein to the tropomyosin-receptor-kinase A (TrkA) is associated with Alzhemeir's development. Owing to the large presence of zinc(II) ions in the synaptic compartments, the zinc ions might be bound to the complex in vivo. Here, we have identified a putative zinc binding site using a combination of computations and experiments. First, we have predicted structural features of the NGF/TrkA complex in aqueous solution by molecular simulation. Metadynamics free energy calculations suggest that these are very similar to those in the X-ray structure...
December 27, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29273827/stimulation-of-intestinal-calcium-absorption-by-orally-administrated-vitamin-d3-compounds-a-prospective-open-label-randomized-trial-in-osteoporosis
#14
K Uenishi, M Tokiwa, S Kato, M Shiraki
Intestinal fractional calcium absorption (FCA) was assessed before and after vitamin D3 treatment. Serum 1,25(OH)2D concentration was significantly increased by plain vitamin D3 and reduced by eldecalcitol. The 1α hydroxyl calcidiol and eldecalcitol treatments increased FCA, which may be induced through direct stimulation of vitamin D receptors in the intestine. INTRODUCTION: To assess the effects of vitamin D3 compounds on intestinal FCA and calcium-regulating hormones in post-menopausal osteoporosis, a randomized open-label prospective study was conducted...
December 23, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/29273483/growth-hormone-receptor-ghr-gene-polymorphism-and-scoliosis-in-prader-willi-syndrome
#15
Merlin G Butler, Waheeda Hossain, Maaz Hassan, Ann M Manzardo
OBJECTIVE: A growth hormone receptor (GHR) gene polymorphism impacts sensitivity to endogenous and exogenous growth hormone (GH) to moderate growth and development. Increased sensitivity may accelerate spinal growth and contribute to scoliosis, particularly in GH-deficient and treated populations such as Prader-Willi syndrome (PWS). Therefore, we examined the relationship between GHR genotype and scoliosis (case and control) in PWS cohorts. DESIGN: We utilized a case-control design in a study of 73 subjects (34M; 39F) with genetically confirmed PWS in 32 individuals previously diagnosed with moderate to severe scoliosis (mean age=16...
December 6, 2017: Growth Hormone & IGF Research
https://www.readbyqxmd.com/read/29243851/alterations-in-gene-expression-in-vitamin-d-deficiency-down-regulation-of-liver-cyp7a1-and-renal-oat3-in-mice
#16
Holly P Quach, Keumhan Noh, Stacie Y Hoi, Adrie Bruinsma, Geny M M Groothuis, Albert P Li, Edwin C Y Chow, K Sandy Pang
The vitamin D-deficient model, established in C57BL/6 mouse after 8-week feeding of vitamin D-deficient diets in absence or presence of added calcium, was found associated with elevated levels of plasma parathyroid hormone (PTH) and plasma and liver cholesterol, and reduction in cholesterol 7α-hydroxylase (Cyp7a1, rate-limiting enzyme for cholesterol metabolism) and renal Oat3 mRNA/protein expression levels. There was, however, no change in plasma calcium and phosphate levels. Appraisal of the liver revealed up-regulation of mRNA expressions of the small heterodimer partner (Shp) and attenuation of Cyp7a1, which contributed to hypercholesterolemia in vitamin D-deficiency...
December 15, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/29234042/cfdna-correlates-with-endothelial-damage-after-cardiac-surgery-with-prolonged-cardiopulmonary-bypass-and-amplifies-netosis-in-an-intracellular-tlr9-independent-manner
#17
Adnana Paunel-Görgülü, Max Wacker, Mouhamed El Aita, Shoreshfan Hassan, Georg Schlachtenberger, Antje Deppe, Yeong-Hoon Choi, Elmar Kuhn, Thorsten O Mehler, Thorsten Wahlers
Cardiopulmonary bypass (CPB) provokes inflammation culminating in organ dysfunction and increased mortality. Recently, neutrophil extracellular traps (NETs) have been found to be involved in a variety of cardiovascular diseases promoting tissue and organ injury. Here, we aimed to elaborate the proinflammatory potential of circulating cell-free (cf)DNA in patients undergoing cardiac surgery with CPB. Plasma was collected pre- and postoperatively as well as at d1, d3, d5 and d8 after surgery. At d1, we found circulating cfDNA levels to be significantly increased in patients with prolonged CPB duration (>100 min) when compared to those with shorter CPB times (CPB < 100 min)...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233620/regulation-of-extracellular-matrix-vesicles-via-rapid-responses-to-steroid-hormones-during-endochondral-bone-formation
#18
REVIEW
Niels Asmussen, Zhao Lin, Michael J McClure, Zvi Schwartz, Barbara D Boyan
Endochondral bone formation is a precise and highly ordered process whose exact regulatory framework is still being elucidated. Multiple regulatory pathways are known to be involved. In some cases, regulation impacts gene expression, resulting in changes in chondrocyte phenotypic expression and extracellular matrix synthesis. Rapid regulatory mechanisms are also involved, resulting in release of enzymes, factors and micro RNAs stored in extracellular matrisomes called matrix vesicles. Vitamin D metabolites modulate endochondral development via both genomic and rapid membrane-associated signaling pathways...
December 9, 2017: Steroids
https://www.readbyqxmd.com/read/29223348/distinctive-binding-properties-of-the-negative-allosteric-modulator-3h-sb269-652-at-recombinant-dopamine-d3-receptors
#19
Irene Fasciani, Ilaria Pietrantoni, Mario Rossi, Clotilde Mannoury la Cour, Gabriella Aloisi, Francesco Marampon, Marco Scarselli, Mark J Millan, Roberto Maggio
Recently, employing radioligand displacement and functional coupling studies, we demonstrated that SB269,652 (N-[(1r,4r)-4-[2-(7-cyano-1,2,3,4-tetrahydroisoquinolin-2-yl)ethyl]cyclohexyl]-1H-indole-2-carboxamide) interacts in an atypical manner with dopamine D3 receptor displaying a unique profile reminiscent of a negative allosteric ligand. Here, we characterized the binding of radiolabelled [3H]SB269,652 to human dopamine D3 receptor stably expressed in Chinese Hamster Ovary cells. Under saturating conditions, SB269,652 showed a KD value of ≈ 1nM...
December 6, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29221778/long-term-treatment-with-dopamine-d3-receptor-agonists-induces-a-behavioral-switch-that-can-be-rescued-by-blocking-the-dopamine-d1-receptor
#20
Mai-Lynne Dinkins, Perrine Lallemand, Stefan Clemens
Restless legs syndrome (RLS) is commonly treated with the dopamine agonists pramipexole, and rotigotine, which target the inhibitory dopamine receptor subtype D3R. While initially highly effective, these compounds lose their efficacy in treating RLS over time, and long-term therapy regularly leads to a worsening of the symptoms (augmentation). This dopamine agonist-induced augmentation has become a prime concern in the treatment of RLS, and while alternate therapies are being proposed, the mechanisms leading to augmentation remain opaque...
December 2017: Sleep Medicine
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