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https://www.readbyqxmd.com/read/28720699/cellular-trade-offs-and-optimal-resource-allocation-during-cyanobacterial-diurnal-growth
#1
Alexandra-M Reimers, Henning Knoop, Alexander Bockmayr, Ralf Steuer
Cyanobacteria are an integral part of Earth's biogeochemical cycles and a promising resource for the synthesis of renewable bioproducts from atmospheric CO2 Growth and metabolism of cyanobacteria are inherently tied to the diurnal rhythm of light availability. As yet, however, insight into the stoichiometric and energetic constraints of cyanobacterial diurnal growth is limited. Here, we develop a computational framework to investigate the optimal allocation of cellular resources during diurnal phototrophic growth using a genome-scale metabolic reconstruction of the cyanobacterium Synechococcus elongatus PCC 7942...
July 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28718632/systematic-approaches-to-efficiently-produce-2-3-butanediol-in-a-marine-cyanobacterium
#2
Nicole E Nozzi, Anna E Case, Austin L Carroll, Shota Atsumi
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues remain in scale-up. Use of the cyanobacterium Synechococcus sp. PCC 7002 (7002) as a chemical production chassis could address a number of these issues given the higher tolerance to salt, light, and heat as well as the fast growth rate of 7002 in comparison to traditional model cyanobacteria such as Synechococcus elongatus PCC 7942 and Synechocystis sp...
July 18, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28680419/novel-synechococcus-genomes-reconstructed-from-freshwater-reservoirs
#3
Pedro J Cabello-Yeves, Jose M Haro-Moreno, Ana-Belen Martin-Cuadrado, Rohit Ghai, Antonio Picazo, Antonio Camacho, Francisco Rodriguez-Valera
Freshwater picocyanobacteria including Synechococcus remain poorly studied at the genomic level, compared to their marine representatives. Here, using a metagenomic assembly approach we discovered two novel Synechococcus sp. genomes from two freshwater reservoirs Tous and Lake Lanier, both sharing 96% average nucleotide identity and displaying high abundance levels in these two lakes located at similar altitudes and temperate latitudes. These new genomes have the smallest estimated size (2.2 Mb) and average intergenic spacer length (20 bp) of any previously sequenced freshwater Synechococcus, which may contribute to their success in oligotrophic freshwater systems...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28667427/variation-of-synechococcus-pigment-genetic-diversity-along-two-turbidity-gradients-in-the-china-seas
#4
Xiaomin Xia, Hongbin Liu, Donghan Choi, Jae Hoon Noh
Synechococcus are important and widely distributed picocyanobacteria that encompass a high pigment diversity. In this study, we developed a primer set (peBF/peAR) for amplifying the cpeBA operon sequence from Synechococcus genomic DNA to study Synechococcus pigment diversity along two turbidity gradients in the China seas. Our data revealed that all previously reported pigment types occurred in the South (SCS) and East (ECS) China Seas. In addition, a novel pigment genetic type (type 3f), represented by the high phycourobilin Synechococcus sp...
July 1, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28667417/enhanced-biomass-and-oxidative-stress-tolerance-of-synechococcus-elongatus-pcc-7942-overexpressing-the-dhar-gene-from-brassica-juncea
#5
Young-Saeng Kim, Il-Sup Kim, Joseph S Boyd, Arnaud Taton, James W Golden, Ho-Sung Yoon
OBJECTIVES: To improve the oxidative stress tolerance, biomass yield, and ascorbate/dehydroascorbate (AsA/DHA) ratio of Synechococcus elongatus PCC 7942 in the presence of H2O2, by heterologous expression of the dehydroascorbate reductase (DHAR) gene from Brassica juncea (BrDHAR). RESULTS: Under H2O2 stress, overexpression of BrDHAR in the transgenic strain (BrD) of S. elongatus greatly increased the AsA/DHA ratio. As part of the AsA recycling system, the oxidative stress response induced by reactive oxygen species was enhanced, and intracellular H2O2 level decreased...
June 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28652895/exploring-components-of-the-co2-concentrating-mechanism-in-alkaliphilic-cyanobacteria-through-genome-based-analysis
#6
Amornpan Klanchui, Supapon Cheevadhanarak, Peerada Prommeenate, Asawin Meechai
In cyanobacteria, the CO2-concentrating mechanism (CCM) is a vital biological process that provides effective photosynthetic CO2 fixation by elevating the CO2 level near the active site of Rubisco. This process enables the adaptation of cyanobacteria to various habitats, particularly in CO2-limited environments. Although CCM of freshwater and marine cyanobacteria are well studied, there is limited information on the CCM of cyanobacteria living under alkaline environments. Here, we aimed to explore the molecular components of CCM in 12 alkaliphilic cyanobacteria through genome-based analysis...
2017: Computational and Structural Biotechnology Journal
https://www.readbyqxmd.com/read/28650444/nutrient-recycling-facilitates-long-term-stability-of-marine-microbial-phototroph-heterotroph-interactions
#7
Joseph A Christie-Oleza, Despoina Sousoni, Matthew Lloyd, Jean Armengaud, David J Scanlan
Biological interactions underpin the functioning of marine ecosystems, be it via competition, predation, mutualism or symbiosis processes. Microbial phototroph-heterotroph interactions propel the engine that results in the biogeochemical cycling of individual elements, and they are critical for understanding and modelling global ocean processes. Unfortunately, studies thus far have focused on exponentially growing cultures in nutrient-rich media, meaning knowledge of such interactions under in situ conditions is rudimentary at best...
June 26, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28634470/the-dark-side-of-the-mushroom-spring-microbial-mat-life-in-the-shadow-of-chlorophototrophs-ii-metabolic-functions-of-abundant-community-members-predicted-from-metagenomic-analyses
#8
Vera Thiel, Michael Hügler, David M Ward, Donald A Bryant
Microbial mat communities in the effluent channels of Octopus and Mushroom Springs within the Lower Geyser Basin of Yellowstone National Park have been extensively characterized. Previous studies have focused on the chlorophototrophic organisms of the phyla Cyanobacteria and Chloroflexi. However, the diversity and metabolic functions of the other portion of the community in the microoxic/anoxic region of the mat are poorly understood. We recently described the diverse but extremely uneven microbial assemblage in the undermat of Mushroom Spring based on 16S rRNA amplicon sequences, which was dominated by Roseiflexus members, filamentous anoxygenic chlorophototrophs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28630913/an-innovative-biologic-system-for-photon-powered-myocardium-in-the-ischemic-heart
#9
Jeffrey E Cohen, Andrew B Goldstone, Michael J Paulsen, Yasuhiro Shudo, Amanda N Steele, Bryan B Edwards, Jay B Patel, John W MacArthur, Michael S Hopkins, Casey E Burnett, Kevin J Jaatinen, Akshara D Thakore, Justin M Farry, Vi N Truong, Alexandra T Bourdillon, Lyndsay M Stapleton, Anahita Eskandari, Alexander S Fairman, William Hiesinger, Tatiana V Esipova, William L Patrick, Keven Ji, Judith A Shizuru, Y Joseph Woo
Coronary artery disease is one of the most common causes of death and disability, afflicting more than 15 million Americans. Although pharmacological advances and revascularization techniques have decreased mortality, many survivors will eventually succumb to heart failure secondary to the residual microvascular perfusion deficit that remains after revascularization. We present a novel system that rescues the myocardium from acute ischemia, using photosynthesis through intramyocardial delivery of the cyanobacterium Synechococcus elongatus...
June 2017: Science Advances
https://www.readbyqxmd.com/read/28630351/evolutionary-radiation-of-lanthipeptides-in-marine-cyanobacteria
#10
Andres Cubillos-Ruiz, Jessie W Berta-Thompson, Jamie W Becker, Wilfred A van der Donk, Sallie W Chisholm
Lanthipeptides are ribosomally derived peptide secondary metabolites that undergo extensive posttranslational modification. Prochlorosins are a group of lanthipeptides produced by certain strains of the ubiquitous marine picocyanobacteria Prochlorococcus and Synechococcus Unlike other lanthipeptide-producing bacteria, picocyanobacteria use an unprecedented mechanism of substrate promiscuity for the production of numerous and diverse lanthipeptides using a single lanthionine synthetase. Through a cross-scale analysis of prochlorosin biosynthesis genes-from genomes to oceanic populations-we show that marine picocyanobacteria have the collective capacity to encode thousands of different cyclic peptides, few of which would display similar ring topologies...
July 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628991/bacterial-community-composition-in-rainwater-associated-with-synoptic-weather-in-an-area-downwind-of-the-asian-continent
#11
Wei Hu, Kotaro Murata, Yuka Horikawa, Ayumi Naganuma, Daizhou Zhang
Bacteria are abundant in atmospheric waters and can be disseminated by precipitation to the surface of the Earth, potentially influencing ecosystems, public health and climate. However, data on bacterial communities in rainwater, especially on the association with weather, are very limited. In this study, rainwater was collected at the coastal city Kumamoto, southwestern Japan, in 2015. The bacterial communities in fourteen samples were identified using 16S rRNA sequencing and compared according to the rain types at the synoptic scale, i...
June 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28622287/a-niche-for-cyanobacteria-producing-chlorophyll-f-within-a-microbial-mat
#12
Satoshi Ohkubo, Hideaki Miyashita
Acquisition of additional photosynthetic pigments enables photosynthetic organisms to survive in particular niches. To reveal the ecological significance of chlorophyll (Chl) f, we investigated the distribution of Chl and cyanobacteria within two microbial mats. In a 7-mm-thick microbial mat beneath the running water of the Nakabusa hot spring, Japan, Chl f was only distributed 4.0-6.5 mm below the surface, where the intensity of far-red light (FR) was higher than that of photosynthetically active radiation (PAR)...
June 16, 2017: ISME Journal
https://www.readbyqxmd.com/read/28618153/cascading-influence-of-inorganic-nitrogen-sources-on-dom-production-composition-lability-and-microbial-community-structure-in-the-open-ocean
#13
S J Goldberg, C E Nelson, D A Viviani, C N Shulse, M J Church
Nitrogen frequently limits oceanic photosynthesis and the availability of inorganic nitrogen sources in the surface oceans is shifting with global change. We evaluated the potential for abrupt increases in inorganic N sources to induce cascading effects on DOM and microbial communities in the surface ocean. We collected water from 5 m depth in the central North Pacific and amended duplicate 20 L polycarbonate carboys with nitrate or ammonium, tracking planktonic carbon fixation, DOM production, DOM composition and microbial community structure responses over 1 week relative to controls...
June 15, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28616951/direct-characterization-of-the-native-structure-and-mechanics-of-cyanobacterial-carboxysomes
#14
Matthew Faulkner, Jorge Rodriguez-Ramos, Gregory F Dykes, Siân V Owen, Selene Casella, Deborah M Simpson, Robert J Beynon, Lu-Ning Liu
Carboxysomes are proteinaceous organelles that play essential roles in enhancing carbon fixation in cyanobacteria and some proteobacteria. These self-assembling organelles encapsulate Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase using a protein shell structurally resembling an icosahedral viral capsid. The protein shell serves as a physical barrier to protect enzymes from the cytosol and a selectively permeable membrane to mediate transport of enzyme substrates and products...
June 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/28611738/identification-of-cyanobacteria-in-a-eutrophic-coastal-lagoon-on-the-southern-baltic-coast
#15
Martin Albrecht, Thomas Pröschold, Rhena Schumann
Cyanobacteria are found worldwide in various habitats. Members of the picocyanobacteria genera Synechococcus and Prochlorococcus dominate in oligotrophic ocean waters. Other picocyanobacteria dominate in eutrophic fresh or brackish waters. Usually, these are morphologically determined as species of the order Chroococcales/clade B2. The phytoplankton of a shallow, eutrophic brackish lagoon was investigated. Phytoplankton was dominated by Aphanothece-like morphospecies year-round for more than 20 years, along a trophy and salinity gradient...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28600876/rerouting-of-carbon-flux-in-a-glycogen-mutant-of-cyanobacteria-assessed-via-isotopically-non-stationary-13-c-metabolic-flux-analysis
#16
John I Hendry, Charulata Prasannan, Fangfang Ma, K Benedikt Möllers, Damini Jaiswal, Madhuri Digmurti, Doug K Allen, Niels-Ulrik Frigaard, Santanu Dasgupta, Pramod P Wangikar
Cyanobacteria, which constitute a quantitatively dominant phylum, have attracted attention in biofuel applications due to favorable physiological characteristics, high photosynthetic efficiency and amenability to genetic manipulations. However, quantitative aspects of cyanobacterial metabolism have received limited attention. In the present study, we have performed isotopically non-stationary (13) C metabolic flux analysis (INST-(13) C-MFA) to analyze rerouting of carbon in a glycogen synthase deficient mutant strain (glgA-I glgA-II) of the model cyanobacterium Synechococcus sp...
June 10, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28595872/distribution-and-diversity-of-marine-picocyanobacteria-community-targeting-of-prochlorococcus-ecotypes-in-winter-conditions-southern-adriatic-sea
#17
Ivana Babić, Ines Petrić, Sunčica Bosak, Hrvoje Mihanović, Iris Dupčić Radić, Zrinka Ljubešić
Adriatic, the northernmost part of the Mediterranean Sea, due its oligotrophy, topography, and hydrology dynamics, and complex circulation patterns, was suggested as an important study site for rapid climatology impacts. Its southern part is mainly oligotrophic and dominated by picophytoplankton, with cyanobacteria as main representatives. Diversity and distribution patterns of different Prochlorococcus ecotypes were investigated by molecular tools and flow cytometry during the winter convection event in the southern Adriatic (BIOTA winter cruise; February/March 2015)...
June 5, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28592992/streamlining-recombination-mediated-genetic-engineering-by-validating-three-neutral-integration-sites-in-synechococcus-sp-pcc-7002
#18
Anne Ilse Maria Vogel, Rahmi Lale, Martin Frank Hohmann-Marriott
BACKGROUND: Synechococcus sp. PCC 7002 (henceforth Synechococcus) is developing into a powerful synthetic biology chassis. In order to streamline the integration of genes into the Synechococcus chromosome, validation of neutral integration sites with optimization of the DNA transformation protocol parameters is necessary. Availability of BioBrick-compatible integration modules is desirable to further simplifying chromosomal integrations. RESULTS: We designed three BioBrick-compatible genetic modules, each targeting a separate neutral integration site, A2842, A0935, and A0159, with varying length of homologous region, spanning from 100 to 800 nt...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28585390/type-4-pili-are-dispensable-for-biofilm-development-in-the-cyanobacterium-synechococcus-elongatus
#19
Elad Nagar, Shaul Zilberman, Eleonora Sendersky, Ryan Simkovsky, Eyal Shimoni, Diana Gershtein, Moshe Herzberg, Susan S Golden, Rakefet Schwarz
The hair-like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubacteria. The protein complex responsible for type IV pilus assembly is homologous with the type II protein secretion complex. In the cyanobacterium Synechococcus elongatus PCC 7942, the gene Synpcc7942_2071 encodes an ATPase homologue of type II/type IV systems. Here, we report that inactivation of Synpcc7942_2071 strongly affected the suite of proteins present in the extracellular milieu (exo-proteome) and eliminated pili observable by electron microscopy...
June 5, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28580015/removal-of-the-product-from-the-culture-medium-strongly-enhances-free-fatty-acid-production-by-genetically-engineered-synechococcus-elongatus
#20
Akihiro Kato, Nobuyuki Takatani, Kazutaka Ikeda, Shin-Ichi Maeda, Tatsuo Omata
BACKGROUND: Cyanobacterial mutants engineered for production of free fatty acids (FFAs) secrete the products to the medium and hence are thought to be useful for biofuel production. The dAS1T mutant constructed from Synechococcus elongatus PCC 7942 has indeed a large capacity of FFA production, which is comparable to that of triacylglycerol production in green algae, but the yield of secreted FFAs is low because the cells accumulate most of the FFAs intracellularly and eventually die of their toxicity...
2017: Biotechnology for Biofuels
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