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Jian Guan, Songdong Shen, Hao Wu, Xin Liu, Weijie Shen, Yuan He, R Duan
The filamentous Cyanobacterium Arthrospira is commercially produced and is a functional, high-value, health food. We identified 5 low temperature and low light intensity tolerant strains of Arthrospira sp. (GMPA1, GMPA7, GMPB1, GMPC1, and GMPC3) using ethyl methanesulfonate mutagenesis and low temperature screening. The 5 Arthrospira strains grew rapidly below 14 °C, 43.75 μmol photons m(-2) s(-1) and performed breed conservation at 2.5 °C, 8.75 μmol photons m(-2) s(-1). We used morphological identification and molecular genetic analysis to identify GMPA1, GMPA7, GMPB1 and GMPC1 as Arthrospira platensis, while GMPC3 was identified as Arthrospira maxima...
February 2017: World Journal of Microbiology & Biotechnology
Christophe Leboulanger, Hélène Agogué, Cécile Bernard, Marc Bouvy, Claire Carré, Maria Cellamare, Charlotte Duval, Eric Fouilland, Patrice Got, Laurent Intertaglia, Céline Lavergne, Emilie Le Floc'h, Cécile Roques, Gérard Sarazin
This study describes, for the first time, the water chemistry and microbial diversity in Dziani Dzaha, a tropical crater lake located on Mayotte Island (Comoros archipelago, Western Indian Ocean). The lake water had a high level of dissolved matter and high alkalinity (10.6-14.5 g L-1 eq. CO32-, i.e. 160-220 mM compare to around 2-2.5 in seawater), with salinity up to 52 psu, 1.5 higher than seawater. Hierarchical clustering discriminated Dziani Dzaha water from other alkaline, saline lakes, highlighting its thalassohaline nature...
2017: PloS One
Patrizia Lo Cascio, Concetta Calabrò, Clara Bertuccio, Irene Paterniti, Deborah Palombieri, Margherita Calò, Ambrogina Albergamo, Andrea Salvo, Maria Gabriella Denaro
In the present work, morphological and molecular effects of short-term feed deprivation and refeeding with Spirulina (Arthrospira platensis) on zebrafish digestive tract were determined. Once elucidated the proximate composition of Spirulina feed, immunohistochemical and western blot analyses of peptide transporter (PepT1) and cholecystokinin (CCK8) were carried out in the gastrointestinal tract of zebrafish, previously morphologically investigated. Two and five fasting days caused not only morphostructural alterations, but also the downregulation of PepT1 and CCK8 proteins...
January 3, 2017: Natural Product Research
Marina Massa, Silvia Buono, Antonio Luca Langellotti, Luigi Castaldo, Anna Martello, Antonello Paduano, Raffaele Sacchi, Vincenzo Fogliano
In this paper, two freshwater microalgae (Tetradesmus obliquus and Botryococcus braunii), a marine diatom (Phaeodactylum tricornutum) and a photosynthetic cyanobacterium (Arthrospira maxima) were investigated for their ability to grow on liquid digestates (LDs). Three LDs were obtained from anaerobic digestion of different organic wastes: zootechnical (ZW LD), vegetable biomass (VW LD) and the organic fraction of municipal solid wastes (MW LD). All the strains showed the same growth performance on VW LD as on the respective standard media (SM), while ZW LD was efficient only for growth of T...
December 30, 2016: New Biotechnology
Malin Hultberg, Olle Lind, Göran Birgersson, Håkan Asp
The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be observed between the treatments in protein amount, amino acid composition, and total lipid concentration. The concentration of the pigment phycocyanin differed significantly between Spirulina medium and the effluent-based medium (63...
December 26, 2016: Bioprocess and Biosystems Engineering
A Srivastava, V K Singh, S Patnaik, J Tripathi, P Singh, G Nath, R K Asthana
AIMS: Explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and MRSA strains) drug resource using bioassay, NRPS (non-ribosomal polypeptide synthetase) and PKS (polyketide synthase) genes, as well as in silico approach. METHODS AND RESULTS: We have bioassayed the extracts of Phormidium CCC727, Geitlerinema CCC728, Arthrospira CCC729, Leptolyngbya CCC732, Phormidium CCC730, Phormidium CCC731 against six pathogenic bacteria Gram (+ve): Staphylococcus aureus including seven clinical methicillin-resistant S...
December 22, 2016: Journal of Applied Microbiology
Wajdi Ben Hania, Manon Joseph, Boyke Bunk, Cathrin Spröer, Hans-Peter Klenk, Marie-Laure Fardeau, Stefan Spring
The anaerobic, mesophilic and moderately halophilic strain L21-Spi-D4(T) was recently isolated from the suboxic zone of a hypersaline cyanobacterial mat using protein-rich extracts of Arthrospira (formerly Spirulina) platensis as substrate. Phylogenetic analyses based on 16S rRNA genes indicated an affiliation of the novel strain with the Bacteroidetes clade MgMjR-022, which is widely distributed and abundant in hypersaline microbial mats and heretofore comprised only sequences of uncultured bacteria. Analyses of the complete genome sequence of strain L21-Spi-D4(T) revealed a possible specialization on the degradation of cyanobacterial biomass...
December 10, 2016: Environmental Microbiology
Marina R S Walther-António, Jun Chen, Francesco Multinu, Alexis Hokenstad, Tammy J Distad, E Heidi Cheek, Gary L Keeney, Douglas J Creedon, Heidi Nelson, Andrea Mariani, Nicholas Chia
BACKGROUND: Endometrial cancer studies have led to a number of well-defined but mechanistically unconnected genetic and environmental risk factors. One of the emerging modulators between environmental triggers and genetic expression is the microbiome. We set out to inquire about the composition of the uterine microbiome and its putative role in endometrial cancer. METHODS: We undertook a study of the microbiome in samples taken from different locations along the female reproductive tract in patients with endometrial cancer (n = 17), patients with endometrial hyperplasia (endometrial cancer precursor, n = 4), and patients afflicted with benign uterine conditions (n = 10)...
November 25, 2016: Genome Medicine
Martí Lecina, Benjamin Sanchez, Carles Solà, Jordi Prat, Mònica Roldán, Mariona Hernández, Ramon Bragós, Carlos J Paredes, Jordi J Cairó
The buoyancy suppression by low energy sonication (LES) treatment (0.8W·mL(-1), 20kHz, 10s) has recently been proposed as an initial harvesting step for Arthrospira sp. This paper aims to describe the structural changes in Arthrospira sp. after LES treatment and to present how these structural changes affect the results obtained by different analytical techniques. Transmission electron microscopy (TEM) micrographs of trichomes evidenced the gas vesicles rupture but also revealed a rearrangement of thylakoids and more visible phycobilisomes were observed...
January 2017: Bioresource Technology
Tamilselvan Abiraman, Ethirajan Ramanathan, Ganapathy Kavitha, Ramasamy Rengasamy, Sengottuvelan Balasubramanian
The synthesis of chitosan capped copper oxide nanoleaves (CCCO NLs) was carried out under three different reaction conditions viz. 1) room temperature, 2) 70°C and 3) high intensity ultrasound (30kHz) sonication method and it has been found that the high intensity ultrasound (30kHz) sonication is the best method when compared to other two methods. The advantages of the present synthetic method are: i) easy one step process, ii) lesser reaction time, iii) good yield, iv) reproducible and v) calcination is not required...
January 2017: Ultrasonics Sonochemistry
Reijiro Nogami, Haruo Nishida, Dang Diem Hong, Minato Wakisaka
A solubility diagram was employed to understand growth promotion of Arthrospira (Spirulina) platensis by steelmaking slag (SMS). The growth promotion effect of 112 % of freshwater microalga A. platensis was obtained using 5 g/L SMS. However, metabolites, such as pigments, and protein content of A. platensis were not significantly affected. Several metals dissolved in Spirulina-Ogawa-Terui medium were detected by inductively coupled plasma atomic emission spectrometry just after the addition of SMS. The solubility diagram provides information on the chemical speciation of metal elements based on pH and concentration...
December 2016: AMB Express
Ganapathy Kavitha, Chidambaram Kurinjimalar, Krishnan Sivakumar, Rajamani Aravind, Chinnadurai Gandhi Shree, Karunanithi Arthi, Perumal Palani, Venkatesan Kaviyarasan, Ramasamy Rengasamy
Six different strains of cyanobacteria were isolated from the freshwater lake, Arakkonam, India. Staining of cells with Nile Red showed the presence of large quantities of PHB granules in the cell cytoplasm of Arthrospira. Molecular identification of the strain was carried out using 16S rRNA analysis and their systematic position was ascertained as Arthrospira platensis RRGK. Studies were carried out on random mutagenesis approach using UV-B radiation for enhancing the production of PHB. Further, Response Surface Methodology was used for optimization of pH, temperature, and sodium bicarbonate for higher biomass and PHB production...
December 2016: International Journal of Biological Macromolecules
Prachi Zawar, Vivek Javalkote, Robert Burnap, Pramod Mahulikar, Pravin Puranik
The present study reports a process wherein CO2 is captured in the form of bicarbonates using calcium oxide and photosynthetically fixed into biomass. Microalgal cultures viz. Chlorella sorokiniana PAZ and Arthrospira sp. VSJ were grown in the medium containing bicarbonates. The rate of bicarbonate utilization by C. sorokiniana PAZ was higher when CO2 trapped in the presence of 2.67mM calcium oxide than in the presence of 10mM sodium hydroxide and with direct addition of 10mM sodium bicarbonate. For Arthrospira sp...
December 2016: Bioresource Technology
Frédéric Deschoenmaeker, Raphaël Facchini, Juan Carlos Cabrera Pino, Guillaume Bayon-Vicente, Neha Sachdeva, Patrick Flammang, Ruddy Wattiez
In cyanobacteria, the nitrogen and carbon metabolisms are functionally bridged and consequently respond to the carbon-to-nitrogen ratio. Consequently, a nitrogen deficiency results in carbon excess. For the first time, the biological adaptation of Arthrospira sp. PCC 8005 to nitrogen starvation has been deeply characterized at the cellular structure scale. The results indicated that the carbon excess is rerouted into carbon storage granules, such as the polyhydroxyalkanoate and glycogen granules corroborating existing data...
December 2016: Journal of Structural Biology
Elier Ekberg Neri-Torres, Jorge J Chanona-Pérez, Hector A Calderón, Neil Torres-Figueredo, German Chamorro-Cevallos, Georgina Calderón-Domínguez, Hugo Velasco-Bedrán
The objective of this work was to obtain Spirulina (Arthrospira maxima) nanoparticles (SNPs) by using high-impact mechanical milling and to characterize them by electron microscopy and spectroscopy techniques. The milling products were analyzed after various processing times (1-4 h), and particle size distribution and number mean size (NMS) were determined by analysis of high-resolution scanning electron microscopic images. The smallest particles are synthesized after 3 h of milling, had an NMS of 55.6±3.6 nm, with 95% of the particles being smaller than 100 nm...
August 2016: Microscopy and Microanalysis
Effat Fahmy Shabana, Mahmoud Ali Gabr, Helal Ragab Moussa, Enas Ali El-Shaer, Mostafa M S Ismaiel
Arthrospira (Spirulina) platensis is a blue-green alga, rich with bioactive components and nutrients. To evaluate effect of gamma irradiation, A. platensis was exposed to different doses of 0.0, 0.5, 1.0, 1.5, 2.0 and 2.5kGy. The data showed that the phenolic and proline contents significantly increased with the increase of gamma irradiation doses up to 2.0kGy, above which a reduction was observed. The soluble proteins and malondialdehyde (MDA) contents were stimulated by all tested irradiation doses. Furthermore, the vitamins (A, K and B group) and mineral contents (N, P, Na, K, Ca, Mg and Fe) were stimulated by the irradiation doses compared with the control...
January 1, 2017: Food Chemistry
Yuemei Wu, Suying Wang, Shirui Dong
OBJECTIVE: Based on 16S rRNA and rpoC1 gene sequences, the phylogenetic relationship between Spirulina and Arthrospira were studied and compared. METHODS: We amplified, sequenced and analyzed 16S rRNA and rpoC1 of 84 strains. Then the phylogenetic trees were constructed and compared. RESULTS: The conserved sites percentage, average G+C content and sequence identity of rpoC1 were 49.7%, 47.7%, 76%-100% respectively, significantly lower than 79...
February 4, 2016: Wei Sheng Wu Xue Bao, Acta Microbiologica Sinica
Gitte S Jensen, Cassandra Drapeau, Miki Lenninger, Kathleen F Benson
The goal for this study was to evaluate safety regarding anticoagulant activity and platelet activation during daily consumption of an aqueous cyanophyta extract (ACE), containing a high dose of phycocyanin. Using a randomized, double-blind, placebo-controlled study design, 24 men and women were enrolled after informed consent, and consumed either ACE (2.3 g/day) or placebo daily for 2 weeks. The ACE dose was equivalent to ∼1 g phycocyanin per day, chosen based on the highest dose Generally Recognized as Safe (GRAS) by the U...
July 2016: Journal of Medicinal Food
Teng Xu, Song Qin, Yongwu Hu, Zhijian Song, Jianchao Ying, Peizhen Li, Wei Dong, Fangqing Zhao, Huanming Yang, Qiyu Bao
Arthrospira platensis is a multi-cellular and filamentous non-N2-fixing cyanobacterium that is capable of performing oxygenic photosynthesis. In this study, we determined the nearly complete genome sequence of A. platensis YZ. A. platensis YZ genome is a single, circular chromosome of 6.62 Mb in size. Phylogenetic and comparative genomic analyses revealed that A. platensis YZ was more closely related to A. platensis NIES-39 than Arthrospira sp. PCC 8005 and A. platensis C1. Broad gene gains were identified between A...
August 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
M Kashith, B Keerthana, S Sriram, V Ramamurthy
Cyclic 3',5' adenosine monophosphate (cAMP) is a ubiquitous signaling molecule, but its role in higher plants was in doubt due to its very low concentration. In this study we wanted to look at the flux of cAMP in response to light in algae, considered to be the more primitive form of photosynthetic organisms. While it did not fluctuate very much in the tested green algae, in the cyanobacterium Arthrospira platensis its level was closely linked to exposure to light. The expression from cyaC, the major isoform of adenylate cyclase was strongly influenced by exposure of the cells to light...
August 19, 2016: Biochemical and Biophysical Research Communications
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