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Sarah Schatschneider, Salah Abdelrazig, Laudina Safo, Anne Meint Henstra, Thomas Millat, Dong-Hyun Kim, Klaus Winzer, Nigel Peter Minton, David A Barrett
We have investigated the applicability of commercially available lyophilised Spirulina ( Arthrospira platensis), a microorganism uniformly labelled with13 C, as a readily accessible source of multiple13 C-labelled metabolites suitable as internal standards for quantitative determination of intracellular bacterial metabolites. Metabolites of interest were analysed by hydrophilic interaction liquid chromatography coupled with high resolution mass spectrometry. Multiple internal standards obtained from uniformly (U)-13 C-labelled extract from Spirulina were used to enable isotope dilution mass spectrometry (IDMS) for the identification and quantification of the intracellular metabolites...
March 13, 2018: Analytical Chemistry
Lauris Del Carmen Mejia-da-Silva, Marcelo Chuei Matsudo, Ana Lucia Morocho-Jacome, João Carlos Monteiro de Carvalho
Since cultivations of Arthrospira platensis have a high water demand, it is necessary to develop treatment methods for reusing the exhausted medium that may prevent environmental problems and obtaining useful biomass. The exhausted Schlösser medium obtained from A. platensis batch cultivation in bench-scale mini-tanks was treated by varying concentrations of different coagulants, ferric chloride (6, 10, and 14 mg L-1 ) or ferric sulfate (15, 25, and 35 mg L-1 ) and powdered activated carbon (PAC, 30 and 50 mg L-1 )...
March 5, 2018: Applied Biochemistry and Biotechnology
Kurt Appel, Eduardo Munoz, Carmen Navarrete, Cristina Cruz-Teno, Andreas Biller, Eva Thiemann
Immulina® , a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis ( Spirulina ) is a potent activator of innate immune cells. On the other hand, it is well documented that Spirulina exerts anti-inflammatory effects and showed promising effects with respect to the relief of allergic rhinitis symptoms. Taking into account these findings, we decided to elucidate whether Immulina® , and immunLoges® (a commercial available multicomponent nutraceutical with Immulina® as a main ingredient) beyond immune-enhancing effects, might also exert inhibitory effects in the induced allergic inflammatory response and on histamine release from RBL-2H3 mast cells...
February 26, 2018: Plants (Basel, Switzerland)
Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
Background: The cyanobacterium Arthrospira platensis shows promise as a carbohydrate feedstock for biofuel production. The glycogen accumulated in A. platensis can be extracted by lysozyme-degrading the peptidoglycan layer of the bacterial cell walls. The extracted glycogen can be converted to ethanol through hydrolysis by amylolytic enzymes and fermentation by the yeast Saccharomyces cerevisiae . Thus, in the presence of lysozyme, a recombinant yeast expressing α-amylase and glucoamylase can convert A...
2018: Biotechnology for Biofuels
Uwe Wollina, Cristiana Voicu, Serena Gianfaldoni, Torello Lotti, Katlein França, Georgi Tchernev
The search for natural products with benefits for health in general and of potential for treating human disease has gained wider interest world-wide. Here, we analyse current data on the microalga Arthrospira platensis (AP), that has been used in nutrition since ancient times in Fare East and African communities, for medical purposes with a focus on dermatology. Extracts of AP have been investigated in vitro and in vivo. The alga is rich in proteins, lipopolysaccharides and gamma-linolenic acid. AP extracts, phycocyanin compounds and polysaccharide calcium spirulan (Ca-SP) have been evaluated in various models...
January 25, 2018: Open Access Macedonian Journal of Medical Sciences
Emmanuel Manirafasha, Theophile Murwanashyaka, Theoneste Ndikubwimana, Nur Rashid Ahmed, Jingyi Liu, Yinghua Lu, Xianhai Zeng, Xueping Ling, Keju Jing
Arthrospira (Spirulina) platensis is known to have high-quality proteins content and phycocyanin as one of the major pigment constituents of the cells, and the most challenging problem associated with phycocyanin production in Arthrospira is to optimize its intracellular accumulation. The present study evaluated the metabolic stress conditions (by nutrient enrichment) of Arthrospira platensis FACHB-314 for boosting biomass growth and high content phycocyanin accumulation. Experimental results showed that 5 mM sodium glutamate and 7...
December 23, 2017: Bioresource Technology
Lucy E Eland, Russell J Davenport, Andre Bezerra Dos Santos, Cesar R Mota Filho
Waste Stabilization Ponds are widely used across the world as a passive wastewater treatment for domestic wastewaters, but are often treated as a 'black box' with little known about their ecology, especially their phototrophic communities. This study uses molecular methods and flow cytometry, including polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and sequencing to assess and quantify the cyanobacterial and eukaryotic communities longitudinally throughout two systems, one treating domestic wastewater and the other mixed industrial/domestic wastewaters...
February 5, 2018: Environmental Technology
Elisabetta Bigagli, Lorenzo Cinci, Alberto Niccolai, Mario R Tredici, Natascia Biondi, Liliana Rodolfi, Maura Lodovici, Mario D'Ambrosio, Giulia Mori, Cristina Luceri
Arthrospira platensis (A. platensis) is worldwide consumed as dietary supplement, but its use in the form of whole biomass for food purposes may raise toxicity concerns. The aim of this study was to preliminarily evaluate the safety of an A. platensis F&M-C256-enriched diet (20% (weight/weight) corresponding to 12g/kg body weight/day), administered to rats for 1month. A. platensis F&M-C256-enriched diet was well tolerated: behavior, body weight, food consumption and growth curves were not affected; no discomfort, no deaths and no physical signs related to the treatment were observed during the administration period; food daily consumption and apparent digestibility were comparable to those of the standard laboratory AIN-76 control diet...
December 2017: Food Research International
Maria Kissoudi, Ioannis Sarakatsianos, Victoria Samanidou
C-Phycocyanin is the major phycobiliprotein in Arthrospira platensis, also known as Spirulina, which is a cyanobacterium used as a dietary supplement because of its powerful effects on body and brain. C-phycocyanin is a blue-colored accessory photosynthetic pigment with multiple applications in food industry as natural dye or additive, and in pharmaceuticals. This study presents a simple protocol for the extraction and purification of food-grade C-phycocyanin from Arthrospira platensis. The cell lysis of cyanobacterium was performed by sonication combined with repeated freezing and thawing cycles...
February 2018: Journal of Separation Science
Geeta G Shirnalli, Manish S Kaushik, Ajay Kumar, Gerard Abraham, Pawan K Singh
Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N-methyl-Ń-Nitro-nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G-1, G-2, and SF) were selected and characterized...
November 17, 2017: Journal of Basic Microbiology
Samah R Khalil, Walaa M Elhady, Yaser H A Elewa, Noura E Abd El-Hameed, Sozan A Ali
Environmental pollutants, particularly metallic elements, mobilized and released into the environment, eventually accumulate in the food chain and thus pose a serious threat to human and animal health. In the present study, the role of Arthrospira (Spirulina platensis; SP) as a protector against oxidative stress-mediated liver damage induced by an exposure to lead acetate (LA; as a metallic pollutant) was assessed. To achieve this aim, rats were orally administered with 300 mg/kg bw SP for 15 days, before and concurrently with an intraperitoneal injection of 50 mg/kg bw LA (6 injections throughout 15 days)...
November 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Manal M A Mahmoud, Maather M M El-Lamie, Omnia E Kilany, Amina A Dessouki
One hundred and eighty Nile tilapia fish were used in eighty-three-day growth trial. Fish were divided into three treatment groups. The first group T0 was given the basal diet without any supplementation and served as the control group. The second group T1 was given the basal diet supplemented by 1% Spirulina. The third group T2 was given the basal diet supplemented by 2% Spirulina. At the end of the growth performance trial, a challenge trial was conducted using virulent strain of Pseudomonas fluorescens. Clinical signs, mortalities, postmortem lesions and histopathological alterations were recorded...
January 2018: Fish & Shellfish Immunology
S Hena, H Znad, K T Heong, S Judd
Dairy cattle treated wastewaters are potential resources for production of microalgae biofuels. A study was conducted to evaluate the capability of Arthrospira platensis cultivated in dairy farm wastewater for biodiesel production. The biomass of Arthrospira platensis was found to be 4.98 g L-1 and produced 30.23 wt% lipids to dry biomass cultivated in wastewater which was found nitrogen stressed in photo bioreactor. The extracted lipid displayed a suitable fatty acid profile for biodiesel, although the content of linolenic acid was found a little higher than the standard EN14214...
January 1, 2018: Water Research
Łukasz Wajda, Aleksandra Duda-Chodak, Tomasz Tarko, Paweł Kamiński
Current study was focused on optimising lead(II) biosorption carried out by living cells of Arthrospira platensis using Principal Component Analysis. Various experimental conditions were considered: initial metal concentration (50 and 100 mg/l), solution pH (4.0, 4.5, 5.0, 5.5) and contact time (10, 20, 30, 40, 50 and 60 min) at constant rotary speed 200 rpm. It was found that when the biomass was separated from experimental solutions by the filtration, almost 50% of initial metal dose was removed by the filter paper...
October 3, 2017: World Journal of Microbiology & Biotechnology
Sara Mirzaie, Fahim Zirak-Khattab, Seyed Abdollah Hosseini, Hamid Donyaei-Darian
Objective: Spirulina has been recognized formerly as a filamentous spiral-shaped blue- green algae or more recently as a genus of photosynthetic bacteria (Arthrospira). This microorganism is considered as a rich source of essential nutrients for human and animals. The present study was conducted to determine potential application of Spirulina for heat- exposed broilers. Methods: Two hundred and fifty Cobb 500 chicks with male to female in equal ratio with average initial weight of 615...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
Sergiy Smetana, Michael Sandmann, Sascha Rohn, Daniel Pleissner, Volker Heinz
The lack of protein sources in Europe could be reduced with onsite production of microalgae with autotrophic and heterotrophic systems, owing the confirmation of economic and environmental benefits. This study aimed at the life cycle assessment (LCA) of microalgae and cyanobacteria cultivation (Chlorella vulgaris and Arthrospira platensis) in autotrophic and heterotrophic conditions on a pilot industrial scale (in model conditions of Berlin, Germany) with further biomass processing for food and feed products...
December 2017: Bioresource Technology
Barbara Szirányi, Gergely Krett, Tünde Kosáros, Endre Janurik, Ferenc Pekár, Károly Márialigeti, Andrea K Borsodi
Disposal of used geothermal waters in Hungary often means temporary storage in reservoir lakes to reduce temperature and improve water quality. In this study, the physical and chemical properties and changes in the bacterial community structure of a reservoir lake system in southeast region of Hungary were monitored and compared through 2 years, respectively. The values of biological oxygen demand, concentrations of ammonium ion, total inorganic nitrogen, total phosphorous, and total phenol decreased, whereas oxygen saturation, total organic nitrogen, pH, and conductivity increased during the storage period...
September 5, 2017: Acta Microbiologica et Immunologica Hungarica
Elia Bari, Carla Renata Arciola, Barbara Vigani, Barbara Crivelli, Paola Moro, Giorgio Marrubini, Milena Sorrenti, Laura Catenacci, Giovanna Bruni, Theodora Chlapanidas, Enrico Lucarelli, Sara Perteghella, Maria Luisa Torre
Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C...
August 23, 2017: Materials
Faten Ben Amor, Mohamed Barkallah, Fatma Elleuch, Nesrine Karkouch, Mouna Dammak, Bruno Baréa, Pierre Villeneuve, Slim Abdelkafi, Imen Fendri
The blue-green microalga, Arthrospira sp., isolated from the sea of Kssour Essef in Mahdia (Tunisia), was purified and then identified both morphologically and genetically based on 16S rRNA gene sequence. Following physicochemical analysis, the prokaryotic microalga tested represented a competitive source of pigments and showed a considerable rate in protein (64%) which was confirmed by FTIR measurement. The lipid content (4%) was quantified by the gravimetric method and the intracellular lipid bodies were detected with the Nile red staining...
July 25, 2017: International Journal of Biological Macromolecules
Samuel Cirés, María Cristina Casero, Antonio Quesada
Cyanotoxins are secondary metabolites produced by cyanobacteria, of varied chemical nature and toxic effects. Although cyanobacteria thrive in all kinds of ecosystems on Earth even under very harsh conditions, current knowledge on cyanotoxin distribution is almost restricted to freshwaters from temperate latitudes. In this review, we bring to the forefront the presence of cyanotoxins in extreme environments. Cyanotoxins have been reported especially in polar deserts (both from the Arctic and Antarctica) and alkaline lakes, but also in hot deserts, hypersaline environments, and hot springs...
July 24, 2017: Marine Drugs
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