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https://www.readbyqxmd.com/read/29149514/isolation-and-characterization-of-high-protein-and-phycocyanin-producing-mutants-of-arthrospira-platensis
#1
Geeta G Shirnalli, Manish S Kaushik, Ajay Kumar, Gerard Abraham, Pawan K Singh
Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N-methyl-Ń-Nitro-nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G-1, G-2, and SF) were selected and characterized...
November 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29145152/possible-role-of-arthrospira-platensis-in-reversing-oxidative-stress-mediated-liver-damage-in-rats-exposed-to-lead
#2
Samah R Khalil, Walaa M Elhady, Yaser H A Elewa, Noura E Abd El-Hameed, Sozan A Ali
Environmental pollutants, particularly metallic elements, mobilized and released into the environment, eventually accumulate in the food chain and thus pose a serious threat to human and animal health. In the present study, the role of Arthrospira (Spirulina platensis; SP) as a protector against oxidative stress-mediated liver damage induced by an exposure to lead acetate (LA; as a metallic pollutant) was assessed. To achieve this aim, rats were orally administered with 300 mg/kg bw SP for 15 days, before and concurrently with an intraperitoneal injection of 50 mg/kg bw LA (6 injections throughout 15 days)...
November 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29117593/spirulina-arthrospira-platensis-supplementation-improves-growth-performance-feed-utilization-immune-response-and-relieves-oxidative-stress-in-nile-tilapia-oreochromis-niloticus-challenged-with-pseudomonas-fluorescens
#3
Manal M A Mahmoud, Maather M M El-Lamie, Omnia E Kilany, Amina A Dessouki
One hundred and eighty Nile tilapia fish were used in eighty-three-day growth trial. Fish were divided into three treatment groups. The first group T0 was given the basal diet without any supplementation and served as the control group. The second group T1 was given the basal diet supplemented by 1% Spirulina. The third group T2 was given the basal diet supplemented by 2% Spirulina. At the end of the growth performance trial, a challenge trial was conducted using virulent strain of Pseudomonas fluorescens. Clinical signs, mortalities, postmortem lesions and histopathological alterations were recorded...
November 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29107911/dairy-farm-wastewater-treatment-and-lipid-accumulation-by-arthrospira-platensis
#4
S Hena, H Znad, K T Heong, S Judd
Dairy cattle treated wastewaters are potential resources for production of microalgae biofuels. A study was conducted to evaluate the capability of Arthrospira platensis cultivated in dairy farm wastewater for biodiesel production. The biomass of Arthrospira platensis was found to be 4.98 g L(-1) and produced 30.23 wt% lipids to dry biomass cultivated in wastewater which was found nitrogen stressed in photo bioreactor. The extracted lipid displayed a suitable fatty acid profile for biodiesel, although the content of linolenic acid was found a little higher than the standard EN14214...
October 28, 2017: Water Research
https://www.readbyqxmd.com/read/28975552/application-of-principal-component-analysis-for-the-optimisation-of-lead-ii-biosorption
#5
Łukasz Wajda, Aleksandra Duda-Chodak, Tomasz Tarko, Paweł Kamiński
Current study was focused on optimising lead(II) biosorption carried out by living cells of Arthrospira platensis using Principal Component Analysis. Various experimental conditions were considered: initial metal concentration (50 and 100 mg/l), solution pH (4.0, 4.5, 5.0, 5.5) and contact time (10, 20, 30, 40, 50 and 60 min) at constant rotary speed 200 rpm. It was found that when the biomass was separated from experimental solutions by the filtration, almost 50% of initial metal dose was removed by the filter paper...
October 3, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28920419/effects-of-dietary-spirulina-on-antioxidant-status-lipid-profile-immune-response-and-performance-characteristics-of-broiler-chickens-reared-under-high-ambient-temperature
#6
Sara Mirzaie, Fahim Zirak-Khattab, Seyed Abdollah Hosseini, Hamid Donyaei-Darian
Objective: Spirulina has been recognized formerly as a filamentous spiral-shaped blue- green algae or more recently as a genus of photosynthetic bacteria (Arthrospira). This microorganism is considered as a rich source of essential nutrients for human and animals. The present study was conducted to determine potential application of Spirulina for heat- exposed broilers. Methods: Two hundred and fifty Cobb 500 chicks with male to female in equal ratio with average initial weight of 615...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28892686/autotrophic-and-heterotrophic-microalgae-and-cyanobacteria-cultivation-for-food-and-feed-life-cycle-assessment
#7
Sergiy Smetana, Michael Sandmann, Sascha Rohn, Daniel Pleissner, Volker Heinz
The lack of protein sources in Europe could be reduced with onsite production of microalgae with autotrophic and heterotrophic systems, owing the confirmation of economic and environmental benefits. This study aimed at the life cycle assessment (LCA) of microalgae and cyanobacteria cultivation (Chlorella vulgaris and Arthrospira platensis) in autotrophic and heterotrophic conditions on a pilot industrial scale (in model conditions of Berlin, Germany) with further biomass processing for food and feed products...
December 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28870090/efficiency-of-temporary-storage-of-geothermal-waters-in-a-lake-system-monitoring-the-changes-of-water-quality-and-bacterial-community-structures
#8
Barbara Szirányi, Gergely Krett, Tünde Kosáros, Endre Janurik, Ferenc Pekár, Károly Márialigeti, Andrea K Borsodi
Disposal of used geothermal waters in Hungary often means temporary storage in reservoir lakes to reduce temperature and improve water quality. In this study, the physical and chemical properties and changes in the bacterial community structure of a reservoir lake system in southeast region of Hungary were monitored and compared through 2 years, respectively. The values of biological oxygen demand, concentrations of ammonium ion, total inorganic nitrogen, total phosphorous, and total phenol decreased, whereas oxygen saturation, total organic nitrogen, pH, and conductivity increased during the storage period...
September 5, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28832540/in-vitro-effectiveness-of-microspheres-based-on-silk-sericin-and-chlorella-vulgaris-or-arthrospira-platensis-for-wound-healing-applications
#9
Elia Bari, Carla Renata Arciola, Barbara Vigani, Barbara Crivelli, Paola Moro, Giorgio Marrubini, Milena Sorrenti, Laura Catenacci, Giovanna Bruni, Theodora Chlapanidas, Enrico Lucarelli, Sara Perteghella, Maria Luisa Torre
Some natural compounds have recently been widely employed in wound healing applications due to their biological properties. One such compound is sericin, which is produced by Bombix mori, while active polyphenols, polysaccharides and proteins are synthetized by Chlorella vulgaris and Arthrospira platensis microalgae. Our hypothesis was that sericin, as an optimal bioactive polymeric carrier for microencapsulation process, could also improve the regenerative effect of the microalgae. A solvent-free extraction method and spray drying technique were combined to obtain five formulations, based on algal extracts (C...
August 23, 2017: Materials
https://www.readbyqxmd.com/read/28751049/cyanobacteria-as-source-of-marine-bioactive-compounds-molecular-specific-detection-based-on-%C3%AE-9-desaturase-gene
#10
Faten Ben Amor, Mohamed Barkallah, Fatma Elleuch, Nesrine Karkouch, Mouna Dammak, Bruno Baréa, Pierre Villeneuve, Slim Abdelkafi, Imen Fendri
The blue-green microalga, Arthrospira sp., isolated from the sea of Kssour Essef in Mahdia (Tunisia), was purified and then identified both morphologically and genetically based on 16S rRNA gene sequence. Following physicochemical analysis, the prokaryotic microalga tested represented a competitive source of pigments and showed a considerable rate in protein (64%) which was confirmed by FTIR measurement. The lipid content (4%) was quantified by the gravimetric method and the intracellular lipid bodies were detected with the Nile red staining...
July 25, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28737704/toxicity-at-the-edge-of-life-a-review-on-cyanobacterial-toxins-from-extreme-environments
#11
REVIEW
Samuel Cirés, María Cristina Casero, Antonio Quesada
Cyanotoxins are secondary metabolites produced by cyanobacteria, of varied chemical nature and toxic effects. Although cyanobacteria thrive in all kinds of ecosystems on Earth even under very harsh conditions, current knowledge on cyanotoxin distribution is almost restricted to freshwaters from temperate latitudes. In this review, we bring to the forefront the presence of cyanotoxins in extreme environments. Cyanotoxins have been reported especially in polar deserts (both from the Arctic and Antarctica) and alkaline lakes, but also in hot deserts, hypersaline environments, and hot springs...
July 24, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28708310/omics-prediction-of-bioactive-peptides-from-the-edible-cyanobacterium-arthrospira-platensis-proteome
#12
Chaofan Ji, Jing Han, Jingbo Zhang, Jing Hu, Yinghuan Fu, Hang Qi, Yue Sun, Chenxu Yu
BACKGROUND: Bioinformatics approaches are widely used to evaluate the prospects of novel protein sources in bioactive peptide research. Edible cyanobacteria are considered as potential protein precursors. However, the abundance of unicellular cyanobacterial proteins is largely unknown and highly dynamic according to the cultivation conditions, which need to be considered in this research field. The objective of this work was to evaluate the protein abundance of Arthrospira platensis, as well as to map the bioactive peptide sequences from the high-abundance proteins of the A...
July 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28701042/comparison-of-protein-extracts-from-various-unicellular-green-sources
#13
COMPARATIVE STUDY
Emma Teuling, Peter A Wierenga, Johan W Schrama, Harry Gruppen
Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w)...
September 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28698278/on-the-origin-of-reverse-transcriptase-using-crispr-cas-systems-and-their-hyperdiverse-enigmatic-spacer-repertoires
#14
Sukrit Silas, Kira S Makarova, Sergey Shmakov, David Páez-Espino, Georg Mohr, Yi Liu, Michelle Davison, Simon Roux, Siddharth R Krishnamurthy, Becky Xu Hua Fu, Loren L Hansen, David Wang, Matthew B Sullivan, Andrew Millard, Martha R Clokie, Devaki Bhaya, Alan M Lambowitz, Nikos C Kyrpides, Eugene V Koonin, Andrew Z Fire
Cas1 integrase is the key enzyme of the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas adaptation module that mediates acquisition of spacers derived from foreign DNA by CRISPR arrays. In diverse bacteria, the cas1 gene is fused (or adjacent) to a gene encoding a reverse transcriptase (RT) related to group II intron RTs. An RT-Cas1 fusion protein has been recently shown to enable acquisition of CRISPR spacers from RNA. Phylogenetic analysis of the CRISPR-associated RTs demonstrates monophyly of the RT-Cas1 fusion, and coevolution of the RT and Cas1 domains...
July 11, 2017: MBio
https://www.readbyqxmd.com/read/28688944/enhancement-of-extracellular-polymeric-substances-eps-production-in-spirulina-arthrospira-sp-by-two-step-cultivation-process-and-partial-characterization-of-their-polysaccharidic-moiety
#15
Imene Chentir, Marwa Hamdi, Amel Doumandji, Abelkader HadjSadok, Hatem Ben Ouada, Moncef Nasri, Mourad Jridi
The interactive effects of light intensity and NaCl concentration were investigated for Spirulina two-step cultivation process using Full Factorial Design. In the experiment interval, light intensity had no effect while the NaCl concentration had significant effect on the enhancement of extracellular polymeric substances (EPS) production. Interestingly, results revealed a significant negative interaction between light and NaCl concentration indicating that high NaCl concentration (40gL(-1)) and low light intensity (10μmol photons m(-2)s(-1)) enhanced the EPS production...
July 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28662573/early-life-diet-affects-host-microbiota-and-later-life-defenses-against-parasites-in-frogs
#16
Sarah A Knutie, Lauren A Shea, Marinna Kupselaitis, Christina L Wilkinson, Kevin D Kohl, Jason R Rohr
Food resources can affect the health of organisms by altering their symbiotic microbiota and affecting energy reserves for host defenses against parasites. Different diets can vary in their macronutrient content and therefore they might favor certain bacterial communities of the host and affect the development and maintenance of the immune system, such as the inflammatory or antibody responses. Thus, testing the effect of diet, especially for animals with wide diet breadths, on host-associated microbiota and defenses against parasites might be important in determining infection and disease risk...
October 1, 2017: Integrative and Comparative Biology
https://www.readbyqxmd.com/read/28652895/exploring-components-of-the-co2-concentrating-mechanism-in-alkaliphilic-cyanobacteria-through-genome-based-analysis
#17
Amornpan Klanchui, Supapon Cheevadhanarak, Peerada Prommeenate, Asawin Meechai
In cyanobacteria, the CO2-concentrating mechanism (CCM) is a vital biological process that provides effective photosynthetic CO2 fixation by elevating the CO2 level near the active site of Rubisco. This process enables the adaptation of cyanobacteria to various habitats, particularly in CO2-limited environments. Although CCM of freshwater and marine cyanobacteria are well studied, there is limited information on the CCM of cyanobacteria living under alkaline environments. Here, we aimed to explore the molecular components of CCM in 12 alkaliphilic cyanobacteria through genome-based analysis...
2017: Computational and Structural Biotechnology Journal
https://www.readbyqxmd.com/read/28645570/quantitative-proteomics-analysis-by-itraq-revealed-underlying-changes-in-thermotolerance-of-arthrospira-platensis
#18
Rong Chang, Bingxin Lv, Bosheng Li
Growth temperature is a critical factor that affects cultivation of Arthrospira platensis which is a type of cyanobacterium widely known as Spirulina that has significant commercial value. To investigate the molecular mechanism underlying the thermotolerance of Spirulina, differential protein expression profiling was carried out using iTRAQ-based quantitative proteomic analysis. This study only analyzed changes in thylakoids. Among the 2085 proteins quantified, 43 differentially expressed proteins were selected based on the fold change cutoff scores of ≥2 or ≤0...
June 20, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28604646/effect-of-supercritical-carbon-dioxide-extraction-parameters-on-the-biological-activities-and-metabolites-present-in-extracts-from-arthrospira-platensis
#19
Diego A Esquivel-Hernández, José Rodríguez-Rodríguez, Sara P Cuéllar-Bermúdez, J Saúl García-Pérez, Elena I Mancera-Andrade, Jade E Núñez-Echevarría, Aura Ontiveros-Valencia, Magdalena Rostro-Alanis, Rebeca M García-García, J Antonio Torres, Wei Ning Chen, Roberto Parra-Saldívar
Arthrospira platensis was used to obtain functional extracts through supercritical carbon dioxide extraction (SFE-CO₂). Pressure (P), temperature (T), co-solvent (CX), static extraction (SX), dispersant (Di) and dynamic extraction (DX) were evaluated as process parameters through a Plackett-Burman design. The maximum extract yield obtained was 7.48 ± 0.15% w/w. The maximum contents of bioactive metabolites in extracts were 0.69 ± 0.09 µg/g of riboflavin, 5.49 ± 0.10 µg/g of α-tocopherol, 524.46 ± 0...
June 12, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28595074/protective-effect-of-arthrospira-platensis-extracts-against-ultraviolet-b-induced-cellular-senescence-through-inhibition-of-dna-damage-and-matrix-metalloproteinase-1-expression-in-human-dermal-fibroblasts
#20
Jeong-Ju Lee, Ki Bbeum Kim, Jina Heo, Dae-Hyun Cho, Hee-Sik Kim, Song Hee Han, Kyu Joong Ahn, In-Sook An, Sungkwan An, Seunghee Bae
Ultraviolet (UV) light exposure causes skin photoaging, which is known to be preventable and controllable by application of UV-protective agents. In this study, we demonstrated, for the first time, that the extract of microalgae Arthrospira platensis has a reverse effect on UV-induced photodamage such as loss of cell viability, cellular senescence, DNA damage, and collagen destruction in dermal fibroblasts. Forty-eight extracts were prepared from the cell biomass by controlling culture light conditions, extract solvents, and disruption methods...
August 2017: Journal of Photochemistry and Photobiology. B, Biology
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