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mycology bacillus

Faria Fatima, Smita Rastogi Verma, Neelam Pathak, Preeti Bajpai
Myconanotechnology, a combination of mycology and nanotechnology that deals with the synthesis of nanoparticles using fungi or their metabolites, has great potential in the area of agriculture owing to the high surface-to-volume ratio and excellent biomedical, electronic, mechanical and physicochemical properties of these myconanoparticles. Extracellular mycosynthesis of Aspergillus flavus (KF934407) silver nanoparticles (AgNPs) was performed, which were produced by redox reaction. Furthermore, the extracellular synthesised AgNPs were characterised by ultraviolet/visible spectrophotometry, differential light scattering (DLS) and transmission electron microscopy...
December 2016: Journal of Global Antimicrobial Resistance
Faria Fatima, Preeti Bajpai, Neelam Pathak, Sarika Singh, Shivam Priya, Smita Rastogi Verma
BACKGROUND: Particulates of nanometers size have occupied a significant area in the field of medicinal and agricultural purposes due to their large surface-to-volume ratio and exceptional physicochemical, electronic and mechanical properties. Myconanotechnology, an interface between mycology and nanotechnology is budding nowadays for nanoparticle-fabrication using fungus or its metabolites. In the present study, we have isolated and characterized a novel phosphate solubilizing fungus B...
February 27, 2015: BMC Microbiology
Faria Fatima, Preeti Bajpai, Neelam Pathak, Sarika Singh, Shivam Priya, Smita Rastogi Verma
BACKGROUND: Particulates of nanometers size have occupied a significant area in the field of medicinal and agricultural purposes due to their large surface-to-volume ratio and exceptional physicochemical, electronic and mechanical properties. Myconanotechnology, an interface between mycology and nanotechnology is budding nowadays for nanoparticle-fabrication using fungus or its metabolites. In the present study, we have isolated and characterized a novel phosphate solubilizing fungus B...
December 2015: BMC Microbiology
Naseh Maleki-Ravasan, Mohammad Ali Oshaghi, Sara Hajikhani, Zahra Saeidi, Amir Ahmad Akhavan, Mohsen Gerami-Shoar, Mohammad Hasan Shirazi, Bagher Yakhchali, Yavar Rassi, Davoud Afshar
BACKGROUND: Microbes particularly bacteria presenting in the gut of haematophagous insects may have an important role in the epidemiology of human infectious disease. METHODS: The microbial flora of gut and surrounding environmental of a laboratory strain of Phlebotomus papatasi, the main vector of Zoonotic Cutaneous Leishmaniasis (ZCL) in the old world, was investigated. Biochemical reactions and 16s rDNA sequencing of the isolated bacteria against 24 sugars and amino acids were used for bacteria species identification...
2014: Journal of Arthropod-borne Diseases
B El Amraoui, M El Amraoui, N Cohen, A Fassouane
In order to search for antifungal from biological origin, we performed a screening of marine microorganisms isolated from seawater, seaweed, sediment and marine invertebrates collected from different coastal areas of the Moroccan Atlantic Ocean. The antifungal activities of these isolates were investigated against the pathogenic yeasts involved in medical mycology. Whole cultures of 34 marine microorganisms were screened for antifungal activities using the method of agar diffusion against four yeasts. The results showed that among the 34 isolates studied, 13 (38%) strains have antifungal activity against at least one out of four yeast species, 11 isolates have anti-Candida albicans CIP 48...
December 2014: Journal de Mycologie Médicale
E A A Duarte, G V Lacerda, T A S de Oliveira, M Brendel, L L Loguercio, J C Cascardo
The increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. This could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. We explored an Atlantic Rainforest soil sample to search for crude glycerol-degrading microorganisms. Microcosms of this soil were established containing minimal medium + 8% crude glycerol (w/w); the biological activity was measured by respirometry. High CO2 levels were found in some of the crude glycerol microcosms, suggesting the activity of microorganisms capable of degrading this residue...
2013: Genetics and Molecular Research: GMR
Nathan D Olson, Jayne B Morrow
BACKGROUND: Quantitative polymerase chain reaction (qPCR) assays used in pathogen detection require rigorous methods development including characterizing DNA extraction products. A DNA extract characterization process is demonstrated using DNA extracted from five different cells types (two Gram-negatives: Escherichia coli, and Burkholderia thailandensis, spores and vegetative cells from the Gram-positive Bacillus cereus, and yeast Saccharomyces cerevisiae) with six different methods. RESULTS: DNA extract quantity (concentration and extraction efficiency) and quality (purity and intactness) varied by cell type and extraction method enabling the demonstration of different DNA characterization methods...
2012: BMC Research Notes
Antonella Amore, Olimpia Pepe, Valeria Ventorino, Leila Birolo, Chiara Giangrande, Vincenza Faraco
Ninety bacteria isolated from raw composting materials were screened for their cellulolytic activity on solid medium containing carboxymethylcellulose. The bacteria producing the highest cellulolytic activity levels were identified by 16S rRNA sequencing as Bacillus licheniformis strain 1, Bacillus subtilis subsp. subtilis strain B7B, Bacillus subtilis subsp. spizizenii strain 6, and Bacillus amyloliquefaciens strain B31C. Cellulase activity production by the most productive strain B. amyloliquefaciens B31C was optimized in liquid culture varying the carbon source...
February 2013: FEMS Microbiology Letters
E V Hudzenko, N V Borzova, L D Varbanets'
Influence ofsome technological parameters of cultivation ofproducers Cryptococcus albidus, Eupenicillinum erubescens, Bacillus sp. on the process of synthesis of extracellular enzyme alpha-L-rhamnosidase has been studied. The authors have determined optimal sources of carbon (0.2-0.3% rhamnose) and nitrogen (0.2% sodium nitrate for C. albidus and E. erubescens and ammonium sulphate for Bacillus sp.) (the ratio 1:2), cultivation temperature (28 degrees C, 25 degrees C, 42 degrees C, respectively) for maximum synthesis of alpha-L-rhamnosidase...
May 2011: Mikrobiolohichnyĭ Zhurnal., Mikrobiologichny Zhurnal
Rebecca McDougal, Shuiying Yang, Arne Schwelm, Alison Stewart, Rosie Bradshaw
Dothistroma septosporum is the causal agent of Dothistroma needle blight of pine trees. A novel green fluorescent protein (GFP)-based screening method was developed to assess the potential of microorganisms for biocontrol of Dothistroma. The screen utilizes GFP expression as an indicator of metabolic activity in the pathogen and hygromycin resistance selection to determine if the interaction is fungistatic or fungicidal. Results suggested that six of eight Trichoderma isolates tested have the potential to control Dothistroma in vitro, via a fungicidal action...
October 2011: Journal of Microbiological Methods
Juan Pedro Russo, Rocío Raffaeli, Stella Maris Ingratta, Patricia Rafti, Silvana Mestroni
UNLABELLED: Case 1: A 17-year-old male rural worker from Bolivia living in La Plata (Argentina) for the past year had a lesion on the flexor side of his right forearm (6 x 4 cm). The lesion was formed by several confluent nodular areas, wine-red in color, some fistulized, with hemopurulent drainage. The area was hot and painless (Figure 1). On physical examination, no regional adenomegalies were reported. The following analyses were requested and results reported. Soft tissue ultrasound: material of solid consistency with layered liquid areas, located in the subcutaneous cellular tissue, with fistulous tract, connecting through superficial planes...
November 2010: Skinmed
Domenico Pangallo, Katarina Chovanová, Alexandra Simonovicová, Peter Ferianc
This study deals with establishing the characteristics of a microbial community isolated from indoor artworks and the surrounding air environment. It is one of the few studies on microbial degradation of indoor artworks. It shows the potential biodegradative risk that can occur if artworks are not exhibited and conserved in an appropriate environment. The microbial community isolated from the indoor artworks and air environment was examined by cultural and molecular methods. Different plate assays were used to screen the biodegradative activity of the isolated microflora: Remazol Brilliant Blue R, phenol red, and Azure B for the ligninolytic properties; Ostazin brilliant red H-3B for cellulose degradation; CaCO3 glucose agar for solubilization activity; and B4 agar for biomineralization...
March 2009: Canadian Journal of Microbiology
G Tupinambá, A J R da Silva, C S Alviano, T Souto-Padron, L Seldin, D S Alviano
AIMS: The purpose of this study was to investigate the antagonistic activity of Paenibacillus polymyxa strain SCE2 against mycotoxigenic fungi and to characterize the antimicrobial compound. METHODS AND RESULTS: Strain SCE2 showed a broad inhibition spectrum against different mycotoxigenic fungi. The crude supernatant obtained from strain SCE2 was filtered with Amicon Diaflo membranes, and the antimicrobial activity was detected in the fraction ranging from 0.5 to 1 kDa...
October 2008: Journal of Applied Microbiology
M A Dhale, S Divakar, S Umesh-Kumar, G Vijayalakshmi
AIM: Characterization of dehydromonacolin-MV2, a bioactive metabolite isolated from Monascus purpureus mutant (CFR 410-11). METHODS AND RESULTS: Chloroform extract of rice, fermented with a hyperpigment-producing mutant of M. purpureus (CFR 410-11) was found to contain metabolites that inhibited the growth of Bacillus, Pseudomonas and Streptococcus in agar gel diffusion assays. The extract inhibited lipid peroxidation and scavenged 2,2-diphenyl-1-pycrylhydrazyl and hydroxyl radicals...
December 2007: Journal of Applied Microbiology
V H Tournas, J S Kohn, E J Katsoudas
In an effort to identify a more suitable antibiotic for utilization in mycological media, 12 food borne fungal species from various genera including Alternaria alternata, Aspergillus flavus, A. niger, Eurotium chevalieri, Fusarium moniliforme, Penicillium sp., Rhizopus stolonifer, Saccharomyces cerevisiae, Candida tropicalis, Geotrichum candidum, Rhodotorula glutinis and Kluyveromyces thermotolerans along with 21 chloramphenicol-resistant bacterial isolates from fresh produce and ATCC cultures of Pseudomonas aeruginosa, P...
August 15, 2007: International Journal of Food Microbiology
Maria Szymankiewicz, Janusz Kowalewski, Maciej Dancewicz
UNLABELLED: The immunosuppression and incorrect function of the natural protection mechanisms are conducive to lower respiratory tract infections. The patients with malignancy, especially with lung cancer, belong to the group at higher risk to develop an infection due to accumulation of numerous risk factors. OBJECTIVE: The aim of this study was to assess the occurrence of bacteria and fungi in materials taken from lower respiratory tract from the patients treated in Thoracic Surgery and Clinical Department of Thoracic Surgery and Tumours from 2000 to 2004...
September 2006: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
Jeffrey C Lord
In 125 years since Metchnikoff proposed the use of Metarhizium anisopliae to control the wheat cockchafer and brought about the first field trials, microbial control has progressed from the application of naturalists' observations to biotechnology and precision delivery. This review highlights major milestones in its evolution and presents a perspective on its current direction. Fungal pathogens, the most eye-catching agents, dominated the early period, but major mycological control efforts for chinch bugs and citrus pests in the US had questionable success, and interest waned...
May 2005: Journal of Invertebrate Pathology
F J Reynaldi, M R De Giusti, A M Alippi
The fungus Ascosphaera apis, the causative agent of chalkbrood disease in honeybee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of 249 aerobic spore-forming bacterial antagonists isolated from honey samples was evaluated. Each isolate was screened against A. apis by a central disk test assay. Ten bacterial strains that showed the best antagonistic effect to A. apis were selected for further study and identified as Bacillus cereus (m363, mv86, mv81, mv75), Bacillus circulans (Fr231, m448b), Bacillus megaterium (m435), Bacillus pumilus (m354), Bacillus subtilis (m329), and Paenibacillus alvei (m321)...
January 2004: Revista Argentina de Microbiología
Yechiel Becker
Thousands of scientists participated in the World of Microbes Congress, which was organized by the International Union of the Microbiological Societies (IUMS) and took place in the Palais de Congres in Paris, France from 27 July to 1 August 2002. The attendees were members of the IUMS divisions of Bacteriology and Applied Microbiology, Mycology, and Virology. In addition to the symposia that were organized by each division, joined plenary symposia were held, which encompassed subjects of common interest to all microbiologists...
December 2002: Virus Genes
Eufrocinio C Marfori, Shin'ichiro Kajiyama, Ei-ichiro Fukusaki, Akio Kobayashi
The dual culture of Trichoderma harzianum and Catharanthus roseus callus produced an antimicrobial compound with a remarkable activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis. Structural elucidation revealed that this compound, which we have named trichosetin, is a novel tetramic acid (2,4-pyrrolidinedione) antibiotic and a homolog of the fungal metabolite equisetin. This compound however, was not produced in the individual culture of T. harzianum or C. roseus callus.
May 2002: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
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