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https://www.readbyqxmd.com/read/28220773/new-crispr-cas-systems-discovered
#1
Hui Yang, Dinshaw J Patel
In bacteria and archaea, CRISPR-Cas adaptive immune systems utilize RNA-guided endonucleases to defend against invasion by foreign nucleic acids of bacteriophage, virus and plasmid origin. In a recent paper published in Nature, Burstein et al. identified the first Cas9 protein in uncultivated archaea and two novel CRISPR-CasX and CRISPR-CasY systems in uncultivated bacteria by capitalizing on analysis of terabase-scale metagenomic datasets from natural uncultivated organisms.
February 21, 2017: Cell Research
https://www.readbyqxmd.com/read/28219127/-sanger-sequencing-for-the-diagnosis-of-spinal-muscular-atrophy-patients-with-survival-motor-neuron-gene-1-compound-heterozygous-mutation
#2
L Yang, Y Y Cao, Y J Qu, J L Bai, H Wang, Y W Jin, Y L Han, F Song
Objective: To detect the subtle variant of survival motor neuron gene 1(SMN1) by Sanger sequencing, and to assess the value of Sanger sequencing for the diagnosis of spinal muscular atrophy(SMA) with compound heterozygous mutation of SMN1. Methods: Fifty-two patients suspected SMA were recruited by the Capital Institute of Pediatrics from Jan.2014 to June.2016. PCR was used for amplifying exon7 of SMN1 and SMN2 in 52 patients. Natural different base peaks on the sequencing chromatogram in the SMN1 and SMN2 within the amplified segments were identified with Sanger DNA sequencing to detect the homozygous deletion or heterozygous deletion of SMN1...
February 14, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28218967/representation-of-critical-natural-capital-in-china
#3
Yihe Lü, Liwei Zhang, Yuan Zeng, Bojie Fu, Charlotte Whitham, Shuguang Liu, Bingfang Wu
Traditional means of assessing representativeness of conservation value in protected areas depend on measures of structural biodiversity. The effectiveness of priority conservation areas at representing critical natural capital (CNC) (i.e., an essential and renewable subset of natural capital) remains largely unknown. We analyzed the representativeness of CNC-conservation priority areas in national nature reserves (i.e., nature reserves under jurisdiction of the central government with large spatial distribution across the provinces) in China with a new biophysical-based composite indicator approach...
February 20, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/28216951/a-qualitative-insight-on-complementary-and-alternative-medicines-used-by-hypertensive-patients
#4
Inas Rifaat Ibrahim, Mohamed Azmi Hassali, Fahad Saleem, Haydar F Al Tukmagi
BACKGROUND: The self-treatment with complementary and alternative medicines (CAMs) in chronic diseases is portraying an expanding trend worldwide. Yet, little is known concerning patients' motives to use CAM in the control of blood pressure. OBJECTIVE: This study aims to explore the self-use of CAM in the management of hypertension and explore patients' attitudes, perceived benefits, and disclosure to the physician. MATERIALS AND METHODS: A qualitative technique was adopted and face-to-face interviews, using a validated interview guide, were carried out among twenty hypertensive patients...
October 2016: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/28215285/perspectives-on-perspective-taking-how-children-think-about-the-minds-of-others
#5
S A J Birch, V Li, T Haddock, S E Ghrear, P Brosseau-Liard, A Baimel, M Whyte
Perspective taking, or "theory of mind," involves reasoning about the mental states of others (e.g., their intentions, desires, knowledge, beliefs) and is called upon in virtually every aspect of human interaction. Our goals in writing this chapter were to provide an overview of (a) the research questions developmental psychologists ask to shed light on how children think about the inner workings of the mind, and (b) why such research is invaluable in understanding human nature and our ability to interact with, and learn from, one another...
2017: Advances in Child Development and Behavior
https://www.readbyqxmd.com/read/28212426/sustainability-of-common-pool-resources
#6
Raja Rajendra Timilsina, Koji Kotani, Yoshio Kamijo
Sustainability has become a key issue in managing natural resources together with growing concerns for capitalism, environmental and resource problems. We hypothesize that the ongoing modernization of competitive societies, which we refer to as "capitalism," affects human nature for utilizing common pool resources, thus compromising sustainability. To test this hypothesis, we design and implement a set of dynamic common pool resource games and experiments in the following two types of Nepalese areas: (i) rural (non-capitalistic) and (ii) urban (capitalistic) areas...
2017: PloS One
https://www.readbyqxmd.com/read/28211141/potential-relocation-of-climatic-environments-suggests-high-rates-of-climate-displacement-within-the-north-american-protection-network
#7
Enric Batllori, Marc-André Parisien, Sean A Parks, Max A Moritz, Carol Miller
Ongoing climate change may undermine the effectiveness of protected area networks in preserving the set of biotic components and ecological processes they harbor, thereby jeopardizing their conservation capacity into the future. Metrics of climate change, particularly rates and spatial patterns of climatic alteration, can help assess potential threats. Here, we perform a continent-wide climate change vulnerability assessment whereby we compare the baseline climate of the protected area network in North America (Canada, USA, México - NAM) to the projected end-of-century climate (2071-2100)...
February 17, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28202070/social-capital-and-job-satisfaction-among-substance-abuse-treatment-employees
#8
Andreas Tsounis, Dimitris Niakas, Pavlos Sarafis
BACKGROUND: Job satisfaction is an important predictor for management and clinical ratios. Although it is accepted that is affected by many aspects, the influence of social capital remains to be determined. The main purpose of the article is to examine the relationship between job satisfaction and individual social capital for employees offering services in the treatment of addiction. METHODS: A cross-sectional study was conducted on 239 employees from 14 therapeutic programs at KETEA (Therapy Center for Dependent Individuals in Greece) (KETHEA)...
February 15, 2017: Substance Abuse Treatment, Prevention, and Policy
https://www.readbyqxmd.com/read/28191026/supplementation-with-dairy-calcium-and-or-flaxseed-fibers-in-conjunction-with-orlistat-augments-fecal-fat-excretion-without-altering-ratings-of-gastrointestinal-comfort
#9
Mette Kristensen, Signe Rømer Juul, Karina Vejrum Sørensen, Janne Kunchel Lorenzen, Arne Astrup
BACKGROUND: Orlistat is a lipase inhibitor which reduced absorption of dietary fat by ~30% thereby inducing a weight loss; however, side effects occur as a consequence of increased colonic fat content. To test the hypothesis that most gastrointestinal side events induced by treatment with orlistat could be prevented/ameliorated by concomitant use of natural dietary components, flaxseed fiber (FF) and/or dairy calcium (Ca), binding liquid fats to more solid complexes. METHODS: A randomized controlled dietary intervention study...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28188241/examination-of-the-influence-of-social-capital-on-depression-in-fragile-families
#10
Neil A Wilmot, Kim Nichols Dauner
BACKGROUND: While it appears that social capital has a positive effect on mental health, most studies have been cross-sectional in nature and/or employ weak measures of social capital or mental health. Even less attention has been paid to vulnerable populations, such as low-income women with children. Thus, our objective was to explore how different dimensions of social capital impact depression in this population. METHODS: We used data from the Fragile Families and Child Wellbeing Study, which has followed a cohort of children born in large US cities to mostly minority, unmarried parents for over 9 years...
March 2017: Journal of Epidemiology and Community Health
https://www.readbyqxmd.com/read/28166305/associative-nature-of-event-participation-dynamics-a-network-theory-approach
#11
Jelena Smiljanić, Marija Mitrović Dankulov
The affiliation with various social groups can be a critical factor when it comes to quality of life of each individual, making such groups an essential element of every society. The group dynamics, longevity and effectiveness strongly depend on group's ability to attract new members and keep them engaged in group activities. It was shown that high heterogeneity of scientist's engagement in conference activities of the specific scientific community depends on the balance between the numbers of previous attendances and non-attendances and is directly related to scientist's association with that community...
2017: PloS One
https://www.readbyqxmd.com/read/28159796/a-model-for-designing-adaptive-laboratory-evolution-experiments
#12
Ryan A LaCroix, Bernhard O Palsson, Adam M Feist
: The occurrence of mutations is a cornerstone of the evolutionary theory of adaptation, capitalizing on the rare chance that a mutation confers a fitness benefit. Natural selection is increasingly being leveraged in laboratory settings for industrial and basic science applications. Despite an increasing deployment, there are no standardized procedures available for designing and performing adaptive laboratory evolution (ALE) experiments. Thus, there is a need to optimize the experimental design, specifically for determining when to consider an experiment complete and for balancing outcomes with available resources (i...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28146303/thermal-tolerance-in-the-keystone-species-daphnia-magna-a-candidate-gene-and-an-outlier-analysis-approach
#13
M Jansen, A N Geerts, A Rago, K I Spanier, C Denis, L De Meester, L Orsini
Changes in temperature have occurred throughout Earth's history. However, current warming trends exacerbated by human activities impose severe and rapid loss of biodiversity. Although understanding the mechanisms orchestrating organismal response to climate change is important, remarkably few studies document their role in nature. This is because only few systems enable the combined analysis of genetic and plastic responses to environmental change over long time-spans. Here, we characterize genetic and plastic responses to temperature increase in the aquatic keystone grazer Daphnia magna combining a candidate gene and an outlier analysis approach...
February 1, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28137329/retrospective-analysis-of-patient-presentations-at-the-sydney-australia-royal-easter-show-from-2012-to-2014
#14
Nathan Crabtree, Shirley Mo, Leon Ong, Thuvarahan Jegathees, Daniel Wei, David Fahey, Jia Jenny Liu
: Introduction Comprehensive studies on the relationship between patient demographics and subsequent treatment and disposition at a single mass-gathering event are lacking. The Sydney Royal Easter Show (SRES; Sydney Olympic Park, New South Wales, Australia) is an annual, 14-day, agricultural mass-gathering event occurring around the Easter weekend, attracting more than 800,000 patrons per year. In this study, patient records from the SRES were analyzed to examine relationships between weather, crowd size, day of week, and demographics on treatment and disposition...
January 31, 2017: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/28127397/synthetic-photosynthetic-consortia-define-interactions-leading-to-robustness-and-photoproduction
#15
Stephanie G Hays, Leo L W Yan, Pamela A Silver, Daniel C Ducat
BACKGROUND: Microbial consortia composed of autotrophic and heterotrophic species abound in nature, yet examples of synthetic communities with mixed metabolism are limited in the laboratory. We previously engineered a model cyanobacterium, Synechococcus elongatus PCC 7942, to secrete the bulk of the carbon it fixes as sucrose, a carbohydrate that can be utilized by many other microbes. Here, we tested the capability of sucrose-secreting cyanobacteria to act as a flexible platform for the construction of synthetic, light-driven consortia by pairing them with three disparate heterotrophs: Bacillus subtilis, Escherichia coli, or Saccharomyces cerevisiae...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28114940/the-role-of-organizational-context-in-moderating-the-effect-of-research-use-on-pain-outcomes-in-hospitalized-children-a-cross-sectional-study
#16
Janet Yamada, Janet E Squires, Carole A Estabrooks, Charles Victor, Bonnie Stevens
BACKGROUND: Despite substantial research on pediatric pain assessment and management, health care professionals do not adequately incorporate this knowledge into clinical practice. Organizational context (work environment) is a significant factor in influencing outcomes; however, the nature of the mechanisms are relatively unknown. The objective of this study was to assess how organizational context moderates the effect of research use and pain outcomes in hospitalized children. METHODS: A cross-sectional survey was undertaken with 779 nurses in 32 patient care units in 8 Canadian pediatric hospitals, following implementation of a multifaceted knowledge translation intervention, Evidence-based Practice for Improving Quality (EPIQ)...
January 23, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28112073/creating-community-resilience-through-elder-led-physical-and-social-infrastructure
#17
Daniel P Aldrich, Emi Kyota
OBJECTIVE: Natural disasters and rapidly aging populations are chronic problems for societies worldwide. We investigated the effects of an intervention in Japan known as Ibasho, which embeds elderly residents in vulnerable areas within larger social networks and encourages them to participate in leadership activities. This project sought to deepen the connections of these elderly residents to society and to build elderly leadership and community capacity for future crises. METHODS: We carried out surveys of participants and nonparticipant residents across the city of Ofunato in Tohoku, Japan, 1 year after the intervention began...
January 23, 2017: Disaster Medicine and Public Health Preparedness
https://www.readbyqxmd.com/read/28107722/microalgae-biorefinery-high-value-products-perspectives
#18
Kit Wayne Chew, Jing Ying Yap, Pau Loke Show, Ng Hui Suan, Joon Ching Juan, Tau Chuan Ling, Duu-Jong Lee, Jo-Shu Chang
Microalgae have received much interest as a biofuel feedstock in response to the uprising energy crisis, climate change and depletion of natural sources. Development of microalgal biofuels from microalgae does not satisfy the economic feasibility of overwhelming capital investments and operations. Hence, high-value co-products have been produced through the extraction of a fraction of algae to improve the economics of a microalgae biorefinery. Examples of these high-value products are pigments, proteins, lipids, carbohydrates, vitamins and anti-oxidants, with applications in cosmetics, nutritional and pharmaceuticals industries...
April 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28101587/factors-limiting-formation-of-community-forestry-enterprises-in-the-southern-mixteca-region-of-oaxaca-mexico
#19
José Antonio Hernández-Aguilar, Héctor Sergio Cortina-Villar, Luis Enrique García-Barrios, Miguel Ángel Castillo-Santiago
Many studies have considered community-based forestry enterprises to be the best option for development of rural Mexican communities with forests. While some of Mexico's rural communities with forests receive significant economic and social benefits from having a community forestry enterprise, the majority have not formed such enterprises. The purpose of this article is to identify and describe factors limiting the formation of community forestry enterprise in rural communities with temperate forests in the Southern Mixteca region of Oaxaca, Mexico...
January 18, 2017: Environmental Management
https://www.readbyqxmd.com/read/28095933/understanding-older-adults-resilience-during-the-brisbane-floods-social-capital-life-experience-and-optimism
#20
Lauren Brockie, Evonne Miller
OBJECTIVE: The purpose of this study was to explore how social capital or the impact of life and previous disaster experience facilitated resilience in older adults who experienced the 2011 and 2013 floods in Brisbane, Australia. METHODS: Data were drawn from in-depth interviews of 10 older adults from Brisbane who were evacuated in both the 2011 and 2013 floods. A combined qualitative approach drawing from the methods of constructivist grounded theory and narrative inquiry was applied and the data were analyzed by using (inductive) line-by-line and axial coding...
January 18, 2017: Disaster Medicine and Public Health Preparedness
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