keyword
MENU ▼
Read by QxMD icon Read
search

Cédric Blanpain

keyword
https://www.readbyqxmd.com/read/29875149/yap-and-taz-are-essential-for-basal-and-squamous-cell-carcinoma-initiation
#1
Maud Debaugnies, Adriana Sánchez-Danés, Sandrine Rorive, Maylis Raphaël, Mélanie Liagre, Marie-Astrid Parent, Audrey Brisebarre, Isabelle Salmon, Cédric Blanpain
YAP and TAZ are key downstream regulators of the Hippo pathway, regulating cell proliferation and differentiation. YAP and TAZ activation has been reported in different cancer types. However, it remains unclear whether they are required for the initiation of major skin malignancies like basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Here, we analyze the expression of YAP and TAZ in these skin cancers and evaluate cancer initiation in knockout mouse models. We show that YAP and TAZ are nuclear and highly expressed in different BCC types in both human and mice...
June 6, 2018: EMBO Reports
https://www.readbyqxmd.com/read/29849070/deciphering-the-cells-of-origin-of-squamous-cell-carcinomas
#2
REVIEW
Adriana Sánchez-Danés, Cédric Blanpain
Squamous cell carcinomas (SCCs) are among the most prevalent human cancers. SCC comprises a wide range of tumours originated from diverse anatomical locations that share common genetic mutations and expression of squamous differentiation markers. SCCs arise from squamous and non-squamous epithelial tissues. Here, we discuss the different studies in which the cell of origin of SCCs has been uncovered by expressing oncogenes and/or deleting tumour suppressor genes in the different cell lineages that compose these epithelia...
May 30, 2018: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/29784918/early-lineage-segregation-of-multipotent-embryonic-mammary-gland-progenitors
#3
Aline Wuidart, Alejandro Sifrim, Marco Fioramonti, Shigeru Matsumura, Audrey Brisebarre, Daniel Brown, Alessia Centonze, Anne Dannau, Christine Dubois, Alexandra Van Keymeulen, Thierry Voet, Cédric Blanpain
The mammary gland is composed of basal cells and luminal cells. It is generally believed that the mammary gland arises from embryonic multipotent progenitors, but it remains unclear when lineage restriction occurs and what mechanisms are responsible for the switch from multipotency to unipotency during its morphogenesis. Here, we perform multicolour lineage tracing and assess the fate of single progenitors, and demonstrate the existence of a developmental switch from multipotency to unipotency during embryonic mammary gland development...
May 21, 2018: Nature Cell Biology
https://www.readbyqxmd.com/read/29736183/universality-of-clone-dynamics-during-tissue-development
#4
Steffen Rulands, Fabienne Lescroart, Samira Chabab, Christopher J Hindley, Nicole Prior, Magdalena K Sznurkowska, Meritxell Huch, Anna Philpott, Cedric Blanpain, Benjamin D Simons
The emergence of complex organs is driven by the coordinated proliferation, migration and differentiation of precursor cells. The fate behaviour of these cells is reflected in the time evolution their progeny, termed clones, which serve as a key experimental observable. In adult tissues, where cell dynamics is constrained by the condition of homeostasis, clonal tracing studies based on transgenic animal models have advanced our understanding of cell fate behaviour and its dysregulation in disease (1, 2). But what can be learned from clonal dynamics in development, where the spatial cohesiveness of clones is impaired by tissue deformations during tissue growth? Drawing on the results of clonal tracing studies, we show that, despite the complexity of organ development, clonal dynamics may converge to a critical state characterized by universal scaling behaviour of clone sizes...
May 2018: Nature Physics
https://www.readbyqxmd.com/read/29670281/identification-of-the-tumour-transition-states-occurring-during-emt
#5
Ievgenia Pastushenko, Audrey Brisebarre, Alejandro Sifrim, Marco Fioramonti, Tatiana Revenco, Soufiane Boumahdi, Alexandra Van Keymeulen, Daniel Brown, Virginie Moers, Sophie Lemaire, Sarah De Clercq, Esmeralda Minguijón, Cédric Balsat, Youri Sokolow, Christine Dubois, Florian De Cock, Samuel Scozzaro, Federico Sopena, Angel Lanas, Nicky D'Haene, Isabelle Salmon, Jean-Christophe Marine, Thierry Voet, Panagiota A Sotiropoulou, Cédric Blanpain
In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in vivo evidence for this idea. Here we screen a large panel of cell surface markers in skin and mammary primary tumours, and identify the existence of multiple tumour subpopulations associated with different EMT stages: from epithelial to completely mesenchymal states, passing through intermediate hybrid states...
April 2018: Nature
https://www.readbyqxmd.com/read/29371425/defining-the-earliest-step-of-cardiovascular-lineage-segregation-by-single-cell-rna-seq
#6
Fabienne Lescroart, Xiaonan Wang, Xionghui Lin, Benjamin Swedlund, Souhir Gargouri, Adriana Sànchez-Dànes, Victoria Moignard, Christine Dubois, Catherine Paulissen, Sarah Kinston, Berthold Göttgens, Cédric Blanpain
Mouse heart development arises from Mesp1 -expressing cardiovascular progenitors (CPs) that are specified during gastrulation. The molecular processes that control early regional and lineage segregation of CPs have been unclear. We performed single-cell RNA sequencing of wild-type and Mesp1 -null CPs in mice. We showed that populations of Mesp1 CPs are molecularly distinct and span the continuum between epiblast and later mesodermal cells, including hematopoietic progenitors. Single-cell transcriptome analysis of Mesp1 -deficient CPs showed that Mesp1 is required for the exit from the pluripotent state and the induction of the cardiovascular gene expression program...
March 9, 2018: Science
https://www.readbyqxmd.com/read/29132145/gene-therapy-transgenic-stem-cells-replace-skin
#7
COMMENT
Mariaceleste Aragona, Cédric Blanpain
No abstract text is available yet for this article.
November 16, 2017: Nature
https://www.readbyqxmd.com/read/29033351/mouse-cutaneous-melanoma-induced-by-mutant-braf-arises-from-expansion-and-dedifferentiation-of-mature-pigmented-melanocytes
#8
Corinna Köhler, David Nittner, Florian Rambow, Enrico Radaelli, Fabio Stanchi, Niels Vandamme, Arianna Baggiolini, Lukas Sommer, Geert Berx, Joost J van den Oord, Holger Gerhardt, Cedric Blanpain, Jean-Christophe Marine
To identify the cells at the origin of melanoma, we combined single-cell lineage-tracing and transcriptomics approaches with time-lapse imaging. A mouse model that recapitulates key histopathological features of human melanomagenesis was created by inducing a BRafV600E-driven melanomagenic program in tail interfollicular melanocytes. Most targeted mature, melanin-producing melanocytes expanded clonally within the epidermis before losing their differentiated features through transcriptional reprogramming and eventually invading the dermis...
November 2, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28813665/lineage-restricted-mammary-stem-cells-sustain-the-development-homeostasis-and-regeneration-of-the-estrogen-receptor-positive-lineage
#9
Alexandra Van Keymeulen, Marco Fioramonti, Alessia Centonze, Gaëlle Bouvencourt, Younes Achouri, Cédric Blanpain
The mammary gland (MG) is composed of different cell lineages, including the basal and the luminal cells (LCs) that are maintained by distinct stem cell (SC) populations. LCs can be subdivided into estrogen receptor (ER)(+) and ER(-) cells. LCs act as the cancer cell of origin in different types of mammary tumors. It remains unclear whether the heterogeneity found in luminal-derived mammary tumors arises from a pre-existing heterogeneity within LCs. To investigate LC heterogeneity, we used lineage tracing to assess whether the ER(+) lineage is maintained by multipotent SCs or by lineage-restricted SCs...
August 15, 2017: Cell Reports
https://www.readbyqxmd.com/read/28512235/identifying-the-niche-controlling-melanocyte-differentiation
#10
REVIEW
Manuel Zocco, Cédric Blanpain
Melanocytes present in hair follicles are responsible for their pigmentation. Melanocyte differentiation and hair pigmentation depend on the stem cell factor (SCF)/c-Kit signaling pathway, but the niche that regulates melanocyte differentiation is not well characterized. In this issue of Genes & Development , Liao and colleagues (pp. 744-756) identify Krox20+ -derived cells of the hair shaft as the niche and the essential source of SCF required for melanocyte maturation. This study delineates the niche factors regulating melanocyte differentiation and hair pigmentation and opens up new avenues to further characterize the cross-talk between the hair follicle and melanocytes that controls melanocyte maintenance and differentiation...
April 15, 2017: Genes & Development
https://www.readbyqxmd.com/read/28248284/defining-stem-cell-dynamics-and-migration-during-wound-healing-in-mouse-skin-epidermis
#11
Mariaceleste Aragona, Sophie Dekoninck, Steffen Rulands, Sandrine Lenglez, Guilhem Mascré, Benjamin D Simons, Cédric Blanpain
Wound healing is essential to repair the skin after injury. In the epidermis, distinct stem cells (SCs) populations contribute to wound healing. However, how SCs balance proliferation, differentiation and migration to repair a wound remains poorly understood. Here, we show the cellular and molecular mechanisms that regulate wound healing in mouse tail epidermis. Using a combination of proliferation kinetics experiments and molecular profiling, we identify the gene signatures associated with proliferation, differentiation and migration in different regions surrounding the wound...
March 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28003314/maintaining-hair-follicle-stem-cell-identity-in-a-dish
#12
COMMENT
Adriana Sánchez-Danés, Cédric Blanpain
No abstract text is available yet for this article.
January 17, 2017: EMBO Journal
https://www.readbyqxmd.com/read/27955754/editorial-overview-the-ins-and-outs-of-stem-cells-in-differentiation-inflammation-disease
#13
EDITORIAL
Cedric Blanpain, Erwin F Wagner
No abstract text is available yet for this article.
December 2016: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/27889319/cell-type-specific-chromatin-states-differentially-prime-squamous-cell-carcinoma-tumor-initiating-cells-for-epithelial-to-mesenchymal-transition
#14
Mathilde Latil, Dany Nassar, Benjamin Beck, Soufiane Boumahdi, Li Wang, Audrey Brisebarre, Christine Dubois, Erwin Nkusi, Sandrine Lenglez, Agnieszka Checinska, Alizée Vercauteren Drubbel, Michael Devos, Wim Declercq, Rui Yi, Cédric Blanpain
Epithelial to mesenchymal transition (EMT) in cancer cells has been associated with metastasis, stemness, and resistance to therapy. Some tumors undergo EMT while others do not, which may reflect intrinsic properties of their cell of origin. However, this possibility is largely unexplored. By targeting the same oncogenic mutations to discrete skin compartments, we show that cell-type-specific chromatin and transcriptional states differentially prime tumors to EMT. Squamous cell carcinomas (SCCs) derived from interfollicular epidermis (IFE) are generally well differentiated, while hair follicle (HF) stem cell-derived SCCs frequently exhibit EMT, efficiently form secondary tumors, and possess increased metastatic potential...
February 2, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/27459053/defining-the-clonal-dynamics-leading-to-mouse-skin-tumour-initiation
#15
Adriana Sánchez-Danés, Edouard Hannezo, Jean-Christophe Larsimont, Mélanie Liagre, Khalil Kass Youssef, Benjamin D Simons, Cédric Blanpain
The changes in cell dynamics after oncogenic mutation that lead to the development of tumours are currently unknown. Here, using skin epidermis as a model, we assessed the effect of oncogenic hedgehog signalling in distinct cell populations and their capacity to induce basal cell carcinoma, the most frequent cancer in humans. We found that only stem cells, and not progenitors, initiated tumour formation upon oncogenic hedgehog signalling. This difference was due to the hierarchical organization of tumour growth in oncogene-targeted stem cells, characterized by an increase in symmetric self-renewing divisions and a higher p53-dependent resistance to apoptosis, leading to rapid clonal expansion and progression into invasive tumours...
August 18, 2016: Nature
https://www.readbyqxmd.com/read/27376578/p53-induces-formation-of-neat1-lncrna-containing-paraspeckles-that-modulate-replication-stress-response-and-chemosensitivity
#16
Carmen Adriaens, Laura Standaert, Jasmine Barra, Mathilde Latil, Annelien Verfaillie, Peter Kalev, Bram Boeckx, Paul W G Wijnhoven, Enrico Radaelli, William Vermi, Eleonora Leucci, Gaëlle Lapouge, Benjamin Beck, Joost van den Oord, Shinichi Nakagawa, Tetsuro Hirose, Anna A Sablina, Diether Lambrechts, Stein Aerts, Cédric Blanpain, Jean-Christophe Marine
In a search for mediators of the p53 tumor suppressor pathway, which induces pleiotropic and often antagonistic cellular responses, we identified the long noncoding RNA (lncRNA) NEAT1. NEAT1 is an essential architectural component of paraspeckle nuclear bodies, whose pathophysiological relevance remains unclear. Activation of p53, pharmacologically or by oncogene-induced replication stress, stimulated the formation of paraspeckles in mouse and human cells. Silencing Neat1 expression in mice, which prevents paraspeckle formation, sensitized preneoplastic cells to DNA-damage-induced cell death and impaired skin tumorigenesis...
August 2016: Nature Medicine
https://www.readbyqxmd.com/read/27284162/quantitative-lineage-tracing-strategies-to-resolve-multipotency-in-tissue-specific-stem-cells
#17
Aline Wuidart, Marielle Ousset, Steffen Rulands, Benjamin D Simons, Alexandra Van Keymeulen, Cédric Blanpain
Lineage tracing has become the method of choice to study the fate and dynamics of stem cells (SCs) during development, homeostasis, and regeneration. However, transgenic and knock-in Cre drivers used to perform lineage tracing experiments are often dynamically, temporally, and heterogeneously expressed, leading to the initial labeling of different cell types and thereby complicating their interpretation. Here, we developed two methods: the first one based on statistical analysis of multicolor lineage tracing, allowing the definition of multipotency potential to be achieved with high confidence, and the second one based on lineage tracing at saturation to assess the fate of all SCs within a given lineage and the "flux" of cells between different lineages...
June 1, 2016: Genes & Development
https://www.readbyqxmd.com/read/27193450/cancer-stem-cells-basic-concepts-and-therapeutic-implications
#18
REVIEW
Dany Nassar, Cédric Blanpain
Different mechanisms contribute to intratumor heterogeneity, including genetic mutations, the microenvironment, and the existence of subpopulations of cancer cells with increased renewal capacity and the ability to recapitulate the heterogeneity found in primary tumors, which are referred to as cancer stem cells (CSCs). In this review, we discuss how the concept of CSCs has been defined, what assays are currently used to define the functional properties of CSCs, what intrinsic and extrinsic mechanisms regulate CSC functions, how plastic CSCs are, and the importance of epithelial-to-mesenchymal transition in conferring CSC properties...
May 23, 2016: Annual Review of Pathology
https://www.readbyqxmd.com/read/27185833/mesp1-controls-the-speed-polarity-and-directionality-of-cardiovascular-progenitor-migration
#19
Giuseppe Chiapparo, Xionghui Lin, Fabienne Lescroart, Samira Chabab, Catherine Paulissen, Lorenzo Pitisci, Antoine Bondue, Cédric Blanpain
During embryonic development, Mesp1 marks the earliest cardiovascular progenitors (CPs) and promotes their specification, epithelial-mesenchymal transition (EMT), and cardiovascular differentiation. However, Mesp1 deletion in mice does not impair initial CP specification and early cardiac differentiation but induces cardiac malformations thought to arise from a defect of CP migration. Using inducible gain-of-function experiments during embryonic stem cell differentiation, we found that Mesp2, its closest homolog, was as efficient as Mesp1 at promoting CP specification, EMT, and cardiovascular differentiation...
May 23, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/26845409/erratum-genomic-landscape-of-carcinogen-induced-and-genetically-induced-mouse-skin-squamous-cell-carcinoma
#20
Dany Nassar, Mathilde Latil, Bram Boeckx, Diether Lambrechts, Cédric Blanpain
No abstract text is available yet for this article.
February 2016: Nature Medicine
keyword
keyword
81512
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"