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https://www.readbyqxmd.com/read/29677546/bacitracin-promotes-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-by-stimulating-the-bone-morphogenetic-protein-2-smad-axis
#1
Hui Li, Bin'en Nie, Zhe Du, Shutao Zhang, Teng Long, Bing Yue
Bacitracin, a widely used metallopeptide antibiotic, has been reported to be locally used in treating wounds without systemic adverse reactions. Our preliminary study showed that bacitracin might enhance the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). The present study investigated whether bacitracin affects the osteogenic differentiation of HBMSCs and the molecular mechanisms involved. The proliferation of HBMSCs in the presence of bacitracin was examined using a cell counting kit-8 (CCK-8) assay...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29676592/pulsed-dye-laser-as-an-adjuvant-treatment-for-discoid-lupus-erythematosus-a-randomized-controlled-trial
#2
Pawinee Rerknimitr, Nucharin Tekacharin, Ratchathorn Panchaprateep, Jade Wititsuwannakul, Pattarin Tangtanatakul, Nattiya Hirankarn, Pravit Asawanonda
PURPOSE: To evaluate the efficacy and safety of PDL for DLE in a double blinded, randomized, controlled fashion. METHOD: Forty-eight DLE lesions from nine patients were recruited. The lesions on one side of the body were randomized into the treatment group and the other side served as a control. Treatments with the PDL (595nm) were delivered every 4 weeks for 4 consecutive months. The patients were evaluated at weeks 0, 4, 8, 12, 16 and 24. Erythema index (EI) and Texture index (TI) were obtained by Antera3D®...
April 20, 2018: Journal of Dermatological Treatment
https://www.readbyqxmd.com/read/29676521/inhibitory-regulation-of-skin-fibrosis-in-systemic-sclerosis-by-apelin-apj-signaling
#3
Yoko Yokoyama, Akiko Sekiguchi, Chisako Fujiwara, Akihiko Uchiyama, Akihito Uehara, Sachiko Ogino, Ryoko Torii, Osamu Ishikawa, Sei-Ichiro Motegi
OBJECTIVE: Apelin/APJ signaling has been identified to regulate cardiac and arterial fibrosis and involved in the pathogenesis of pulmonary arterial hypertension. Objective was to elucidate the role of apelin in skin fibrosis in systemic sclerosis (SSc). METHODS: Expressions of apelin/APJ in normal and SSc fibroblasts were compared. Effects of siRNA depletion and the addition of apelin in fibroblasts were analyzed. Effect of apelin injections on bleomycin-induced dermal fibrosis in mice was investigated...
April 20, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29676133/-psoralen-inhibits-raw264-7-differentiation-into-osteoclasts-and-bone-resorption-by-regulating-cd4-t-cell-differentiation
#4
Jin-Ping Li, Bao-Ping Xie, Wen-Juan Zhang, Li-Ying Shi, Wei-Juan Li, Ying Zeng, Guo-Xing Gan, Yu-Hong Li
This paper aimed to investigate whether psoralen inhibits the differentiation and bone resorption by regulating CD4+T cell differentiation in RANKL-induced osteoclastogenesis in RAW264.7 cells, and elucidate its mechanism for osteoporosis. CD4+T cells were isolated from spleen cells of Balb/c mice by immunomagnetic separation method. The cells were divided into blank control group and psoralen group. The cells were cultured in 24-well plates and cultured for 3 days, and then they were collected for co-culture experiments after 4 days...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676074/immune-characterization-of-bone-marrow-derived-models-of-mucosal-and-connective-tissue-mast-cells
#5
Sara Benedé, Evan Cody, Charuta Agashe, M Cecilia Berin
PURPOSE: It is well appreciated that mast cells (MCs) demonstrate tissue-specific imprinting, with different biochemical and functional properties between connective tissue MCs (CTMCs) and mucosal MCs (MMCs). Although in vitro systems have been developed to model these different subsets, there has been limited investigation into the functional characteristics of the 2 major MC subsets. Here, we report the immunologic characterization of 2 MCs subsets developed in vitro from bone marrow progenitors modeling MMCs and CTMCs...
May 2018: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/29676073/overexpression-of-mir-155-5p-inhibits-the-proliferation-and-migration-of-il-13-induced-human-bronchial-smooth-muscle-cells-by-suppressing-tgf-%C3%AE-activated-kinase-1-map3k7-binding-protein-2
#6
Yujia Shi, Xingli Fu, Qi Cao, Zhengdao Mao, Yi Chen, Yun Sun, Zhiguang Liu, Qian Zhang
PURPOSE: Molecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism. METHODS: The effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated...
May 2018: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/29675725/effect-of-pycnogenol%C3%A2-on-an-experimental-rat-model-of-allergic-conjunctivitis
#7
Ayse Ipek Akyuz Unsal, Tolga Kocaturk, Ceren Gunel, Ibrahim Meteoglu, Imran Kurt Omurlu, Harun Cakmak, Buket Demirci
BACKGROUND: Allergic conjunctivitis (AC) is a frequent and challenging disease in ophthalmology practice. Cell protective effect of Pycnogenol® (PYC) depends on its antioxidant and anti-inflammatory properties. The aim of the study is to investigate the effect of PYC on an experimental AC model. METHODS: Ovalbumin and Al(OH)3 were given seven times intraperitoneally (i.p.) every other day and ovalbumin installed everyday directly on conjunctiva to create an AC rat model...
April 20, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29675024/activation-of-the-absent-in-melanoma-2-inflammasome-in-peripheral-blood-mononuclear-cells-from-idiopathic-pulmonary-fibrosis-patients-leads-to-the-release-of-pro-fibrotic-mediators
#8
Michela Terlizzi, Antonio Molino, Chiara Colarusso, Chantal Donovan, Pasquale Imitazione, Pasquale Somma, Rita P Aquino, Philip M Hansbro, Aldo Pinto, Rosalinda Sorrentino
Idiopathic pulmonary fibrosis (IPF) is a chronic fibro-proliferative disease characterized by poor prognosis, with a mean survival of ~2-3 years after definite diagnosis. The cause of IPF is still unknown but it is a heterogeneous condition in which the aberrant deposition of extracellular matrix leads to extensive lung remodeling. This remodeling is a consequence of inflammatory responses, but the mechanisms involved are poorly understood. In this study, we first analyzed a bleomycin-induced mouse model, which showed that higher expression of IL-1β, but not IL-18, was correlated to pulmonary cell infiltration and fibrosis...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29674727/acute-hyperthermia-inhibits-tgf-%C3%AE-1-induced-cardiac-fibroblast-activation-via-suppression-of-akt-signaling
#9
Masatoshi Narikawa, Masanari Umemura, Ryo Tanaka, Takayuki Fujita, Utako Yokoyama, Tomoaki Ishigami, Kazuo Kimura, Kouichi Tamura, Yoshihiro Ishikawa
Transforming growth factor-β1 (TGF-β1) induces phenotypic changes in fibroblasts to become myofibroblasts with increased production of extracellular matrix (ECM) components and cytokines. It is also known that excessive activation of myofibroblasts accelerates cardiac fibrosis, remodeling, and thus cardiac dysfunction. However, no effective therapy has been established to prevent this process although recent clinical studies have demonstrated the effectiveness of hyperthermia in cardiac dysfunction. The aim of this study was to examine the molecular mechanism of hyperthermia on TGF-β1-mediated phenotypic changes in cardiac fibroblasts...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29674691/aloperine-protects-mice-against-bleomycin-induced-pulmonary-fibrosis-by-attenuating-fibroblast-proliferation-and-differentiation
#10
Wanling Yin, Jing Han, Zhijun Zhang, Zaomu Han, Siyuan Wang
Aloperine is a quinolizidine alkaloid extracted from Sophora alopecuroides. It has been proven to alleviate oxidative stress and effectively promote tumor cell apoptosis in mice. Herein, we investigated whether aloperine could also mediate its protective effects on bleomycin (BLM)-induced pulmonary fibrosis. Pathological staining, western blot, RT-PCR and flow cytometry were used to evaluate the impact of aloperine on the development of pulmonary fibrosis. The effect of aloperine on fibroblast proliferation, differentiation and related signaling pathways were next investigated to demonstrate the underlying mechanisms...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29673026/in-vitro-effects-of-seng-and-tgf-%C3%AE-on-human-umbilical-vein-endothelial-cells-and-trophoblasts
#11
Ketao Wang, Caihua Lu, Yanpo Liu, Ye Tao
AIM: The aim of this study was to evaluate the in vitro effects of sEng and TGF-β on human umbilical vein endothelial cells (HUVECs) and trophoblasts. METHODS: HUVECs and trophoblasts were treated with 1, 5, 10, 15, 30, 50, 80 and 100 μg/L sEng, TGF-β or (sEng + TGF-β) for 6, 12, 24, 36, 48 and 72 h, respectively. sEng, TGF-β and NO levels in culture media of HUVECs were measured by ELISA. Survival rates of HUVECs and trophoblasts were detected by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) assay...
April 19, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29671649/lag-3-represents-a-marker-of-cd4-t-cells-with-regulatory-activity-in-patients-with-bone-fracture
#12
Jun Wang, Yunfan Ti, Yicun Wang, Guodong Guo, Hui Jiang, Menghan Chang, Hongbo Qian, Jianning Zhao, Guojing Sun
The lymphocyte activation gene 3 (LAG-3) is a CD4 homolog with binding affinity to MHC class II molecules. It is thought that LAG-3 exerts a bimodal function, such that co-ligation of LAG-3 and CD3 could deliver an inhibitory signal in conventional T cells, whereas, on regulatory T cells, LAG-3 expression could promote their inhibitory function. In this study, we investigated the role of LAG-3 expression on CD4+ T cells in patients with long bone fracture. We found that LAG-3+ cells represented approximately 13% of peripheral blood CD4+ T cells on average...
April 19, 2018: Immunological Investigations
https://www.readbyqxmd.com/read/29670673/pm2-5-induced-the-expression-of-fibrogenic-mediators-via-hmgb1-rage-signaling-in-human-airway-epithelial-cells
#13
Weifeng Zou, Fang He, Sha Liu, Jinding Pu, Jinxing Hu, Qing Sheng, Tao Zhu, Tianhua Zhu, Bing Li, Pixin Ran
Background: The aim of the present study was to test whether fine particulate matter (PM2.5) induces the expression of platelet-derived growth factor-AB (PDGF-AB), PDGF-BB, and transforming growth factor- β 1 (TGF- β 1) in human bronchial epithelial cells (HBECs) in vitro via high-mobility group box 1 (HMGB1) receptor for advanced glycation end products (RAGE) signaling. Methods: Sprague-Dawley rats were exposed to motor vehicle exhaust (MVE) or clean air. HBECs were either transfected with a small interfering RNA (siRNA) targeting HMGB1 or incubated with anti-RAGE antibodies and subsequently stimulated with PM2...
2018: Canadian Respiratory Journal: Journal of the Canadian Thoracic Society
https://www.readbyqxmd.com/read/29670670/tgf-%C3%AE-polymorphisms-are-a-risk-factor-for-chagas-disease
#14
Roberto Rodrigues Ferreira, Fabiana da Silva Madeira, Gabriel Farias Alves, Mayara da Costa Chambela, Eduardo de Oliveira Vaz Curvo, Aline Dos Santos Moreira, Renata Almeida de Sá, Leila Mendonça-Lima, Pedro Hernan Cabello, Sabine Bailly, Jean-Jacques Feige, Tania Cremonini Araujo-Jorge, Roberto Magalhães Saraiva, Mariana Caldas Waghabi
Transforming growth factor β 1 (TGF- β 1) is an important mediator in Chagas disease. Furthermore, patients with higher TGF- β 1 serum levels show a worse clinical outcome. Gene polymorphism may account for differences in cytokine production during infectious diseases. We tested whether TGFB1 polymorphisms could be associated with Chagas disease susceptibility and severity in a Brazilian population. We investigated five single-nucleotide polymorphisms (-800 G>A, -509 C>T, +10 T>C, +25 G>C, and +263 C>T)...
2018: Disease Markers
https://www.readbyqxmd.com/read/29670655/odontoblast-like-cells-differentiated-from-dental-pulp-stem-cells-retain-their-phenotype-after-subcultivation
#15
Paula A Baldión, Myriam L Velandia-Romero, Jaime E Castellanos
Odontoblasts, the main cell type in teeth pulp tissue, are not cultivable and they are responsible for the first line of response after dental restauration. Studies on dental materials cytotoxicity and odontoblast cells physiology require large quantity of homogenous cells retaining most of the phenotype characteristics. Odontoblast-like cells (OLC) were differentiated from human dental pulp stem cells using differentiation medium (containing TGF- β 1), and OLC expanded after trypsinization (EXP-21) were evaluated and compared...
2018: International Journal of Cell Biology
https://www.readbyqxmd.com/read/29670368/downregulation-of-mir-542-3p-promotes-cancer-metastasis-through-activating-tgf-%C3%AE-smad-signaling-in-hepatocellular-carcinoma
#16
Tong Zhang, Wei Liu, Wei Meng, Hui Zhao, Qing Yang, Shi-Jie Gu, Cui-Cui Xiao, Chang-Chang Jia, Bin-Sheng Fu
Introduction: Hepatocellular carcinoma (HCC) accounts for more than 90% of primary liver cancer. Although great progress has been made on HCC molecular mechanism and therapy techniques, the prognosis of HCC patient is poor due to high metastasis and recurrence. Materials and methods: Expression of miR-542-3p was quantified by quantitative real-time PCR (qRT-PCR). The role of miR-542-3p in HCC metastasis was examined using transwell and 3D-culture assay. qRT-PCR, Western blotting and luciferase reporter assay were used to elucidate the mechanisms of miR-542-3p-mediated cancer metastasis...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29670207/homeostatic-proliferation-leads-to-telomere-attrition-and-increased-pd-1-expression-after-autologous-hematopoietic-sct-for-systemic-sclerosis
#17
Lucas C M Arruda, João R Lima-Júnior, Emmanuel Clave, Daniela A Moraes, Corinne Douay, Isabelle Fournier, Hélène Moins-Teisserenc, Dimas T Covas, Belinda P Simões, Dominique Farge, Antoine Toubert, Kelen C R Malmegrim, Maria Carolina Oliveira
In the months that follow autologous hematopoietic stem cell transplantation (AHSCT), lymphopenia drives homeostatic proliferation, leading to oligoclonal expansion of residual cells. Here we evaluated how replicative senescent and exhausted cells associated with clinical outcomes of 25 systemic sclerosis (SSc) patients who underwent AHSCT. Patients were clinically monitored for skin (modified Rodnan's skin score, mRSS) and internal organ involvement and had blood samples collected before and semiannually, until 3 years post-AHSCT, for quantification of telomere length, CD8+ CD28- and PD-1+ cells, and serum cytokines...
April 18, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29669741/shp-1-regulates-hematopoietic-stem-cell-quiescence-by-coordinating-tgf-%C3%AE-signaling
#18
Linjia Jiang, Xue Han, Jin Wang, Chen Wang, Xiaoqiang Sun, Jiayi Xie, Guojin Wu, Hiep Phan, Zhenguo Liu, ChengCheng Zhang, Meng Zhao, Xunlei Kang
Cell cycle quiescence is critical for hematopoietic stem cell (HSC) maintenance. TGF-β signaling in bone marrow niche has been identified in regulating HSC quiescence; however, the intrinsic regulatory mechanisms remain unclear. This study reports that Shp-1 knockout HSCs have attenuated quiescence and impaired long-term self-renewal. SHP-1-activated HSCs are surrounded by megakaryocytes, which regulate HSC quiescence by producing TGF-β1. Mechanistically, SHP-1 interacts with the immunoreceptor tyrosine-based inhibition motif on TGF-β receptor 1 and is critical for TGF-β signaling activation in HSCs...
April 18, 2018: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29669342/nr4a1-is-involved-in-fibrogenesis-in-ovarian-endometriosis
#19
Xinliu Zeng, Zhang Yue, Ying Gao, Guosong Jiang, Fuqing Zeng, Ying Shao, Jiayu Huang, Minuo Yin, Yajie Li
BACKGROUND/AIMS: Excess fibrosis may lead to chronic pain, scarring, and infertility as endometriosis develops and progresses. The pathogenesis of endometriosis has been linked to transforming growth factor-β (TGF-β), the most potent promoter of fibrosis. METHODS: Levels of NR4A1 and P-NR4A1 protein in human endometrial and endometriotic tissue were assessed by western blotting and immunohistochemistry. The expression levels of fibrotic markers in stromal cells were evaluated by real-time PCR...
April 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29669252/t-cell-receptor-regulated-tgf-%C3%AE-type-i-receptor-expression-determines-t-cell-quiescence-and-activation
#20
Eric Tu, Cheryl P Z Chia, Weiwei Chen, Dunfang Zhang, Sang A Park, Wenwen Jin, Dandan Wang, Maria-Luisa Alegre, Ying E Zhang, Lingyun Sun, WanJun Chen
It is unclear how quiescence is enforced in naive T cells, but activation by foreign antigens and self-antigens is allowed, despite the presence of inhibitory signals. We showed that active transforming growth factor β (TGF-β) signaling was present in naive T cells, and T cell receptor (TCR) engagement reduced TGF-β signaling during T cell activation by downregulating TGF-β type 1 receptor (TβRI) through activation of caspase recruitment domain-containing protein 11 (CARD11) and nuclear factor κB (NF-κB)...
April 17, 2018: Immunity
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