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https://www.readbyqxmd.com/read/28214889/regulatory-role-of-mirna-375-in-expression-of-bmp15-gdf9-receptors-and-its-effect-on-proliferation-and-apoptosis-of-bovine-cumulus-cells
#1
Hongyan Chen, Chang Liu, Hao Jiang, Yan Gao, Mingqiang Xu, Jiawei Wang, Siyuan Liu, Yao Fu, Xulei Sun, Jiajun Xu, Jiabao Zhang, Lisheng Dai
BACKGROUND: Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are members of the transforming growth factor beta (TGF-β) superfamily. Through autocrine and paracrine mechanisms, these two factors can regulate cell differentiation, proliferation, and other functions in the ovary locally. Furthermore, GDF9 and BMP15 play vital roles in follicular growth, atresia, ovulation, fertilization, reproduction, and maintenance. Numerous studies have demonstrated a synergy between BMP15 and GDF9...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214843/metabolites-of-hypoxic-cardiomyocytes-induce-the-migration-of-cardiac-fibroblasts
#2
Huairui Shi, Xuehong Zhang, Zekun He, Zhiyong Wu, Liya Rao, Yushu Li
BACKGROUND: The migration of cardiac fibroblasts to the infarct region plays a major role in the repair process after myocardial necrosis or damage. However, few studies investigated whether early hypoxia in cardiomyocytes induces the migration of cardiac fibroblasts. The purpose of this study was to assess the role of metabolites of early hypoxic cardiomyocytes in the induction of cardiac fibroblast migration. METHODS: Neonatal rat heart tissue was digested with a mixture of trypsin and collagenase at an appropriate ratio...
January 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214835/up-regulation-of-tgf-%C3%AE-promotes-tendon-to-bone-healing-after-anterior-cruciate-ligament-reconstruction-using-bone-marrow-derived-mesenchymal-stem-cells-through-the-tgf-%C3%AE-mapk-signaling-pathway-in-a-new-zealand-white-rabbit-model
#3
Rui Wang, Bin Xu, Hong-Gang Xu
BACKGROUND/AIMS: This study aimed to explore the role of TGF-β in tendon-to-bone healing after anterior cruciate ligament (ACL) reconstruction using bone marrow-derived mesenchymal stem cells (BMSCs) through the TGF-β/MAPK signaling pathway in a New Zealand white rabbit model. METHODS: A total of 72 healthy male New Zealand white rabbits were selected for these experiments. Flow cytometry and immunofluorescence were used to detect the expression of BMSC surface markers, and qRT-PCR was performed to detect TGF-β mRNA expression...
January 20, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214833/nogo-b-facilitates-lps-mediated-immune-responses-by-up-regulation-of-tlr4-signaling-in-macrophage-raw264-7
#4
Ying Zhu, Qiang Tong, Jia Ye, Yunye Ning, Ye Xiong, Meng Yang, Hua Xiao, Jian Lu, Wujian Xu, Jiandong Li, Qiang Li
BACKGROUND/AIMS: Nogo-B, a member of the reticulon family of proteins, is mainly located in the endoplasmic reticulum (ER). Here, we investigate the function and mechanism of Nogo-B in the regulation of TLR4-associated immune responses in the macrophage cell line of RAW264.7. METHODS: Nogo-B was up- and down-regulated through the use of appropriate adenoviral vectors or siRNA, and the effects of Nogo-B on macrophages under liposaccharide (LPS) stimulation were evaluated via western blotting, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), flow cytometric analysis, and transwell assay...
January 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214509/haplodeficiency-of-activin-receptor-like-kinase-4-alleviates-myocardial-infarction-induced-cardiac-fibrosis-and-preserves-cardiac-function
#5
Yi-He Chen, Qian Wang, Chang-Yi Li, Jian-Wen Hou, Xiao-Meng Chen, Qing Zhou, Jie Chen, Yue-Peng Wang, Yi-Gang Li
Cardiac fibrosis (CF), a repairing process following myocardial infarction (MI), is characterized by abnormal proliferation of cardiac fibroblasts and excessive deposition of extracellular matrix (ECM) resulting in inevitable resultant heart failure. TGF-β (transforming growth factor-β)/ALK5 (Activin receptor-like kinase 5)/Smad2/3/4 pathways have been reported to be involved in the process. Recent studies have implicated both activin and its specific downstream component ALK4 in stimulating fibrosis in non-cardiac organs...
February 15, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28214472/bmp-12-activates-tenogenic-pathway-in-human-adipose-stem-cells-and-affects-their-immunomodulatory-and-secretory-properties
#6
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Agnieszka Kulesza, Kamila Gala, Beata Kaleta, Katarzyna Zielniok, Katarzyna Siennicka, Marek Sabat, Leszek Paczek
BACKGROUND: Cell-based therapy is a treatment method in tendon injuries. Bone morphogenic protein 12 (BMP-12) possesses tenogenic activity and was proposed as a differentiating factor for stem cells directed to transplantation. However, BMPs belong to pleiotropic TGF-β superfamily and have diverse effect on cells. Therefore, the aim of this study was to determine if BMP-12 induces tenogenic differentiation of human adipose stem cells (hASCs) and how it affects other features of this population...
February 18, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28214206/involvement-of-prolyl-isomerase-pin1-in-the-cell-cycle-progression-and-proliferation-of-hepatic-oval-cells
#7
Prabodh Risal, Nirajan Shrestha, Lokendra Chand, Karl G Sylvester, Yeon Jun Jeong
Liver regenerates remarkably after toxic injury or surgical resection. In the case of failure of resident hepatocytes to restore loss, repopulation is carried out by induction, proliferation, and differentiation of the progenitor cell. Although, some signaling pathways have been verified to contribute oval cell-mediated liver regeneration, role of Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(Pin1) in the oval cells proliferation is unknown. In the present study, we evaluate the role of Pin1 in oval cells proliferation...
January 18, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28214084/il-10-and-tgf-%C3%AE-roles-in-chondroprotective-effects-of-glucosamine-in-experimental-osteoarthritis
#8
Nermien E Waly, Abeer Refaiy, Nora M Aborehab
OBJECTIVE: Osteoarthritis (OA) is a complex disease of the whole joint. Glucosamine (GlcN) treatment may have a chondroprotective effect on OA. We investigated the mechanism of action of glucosamine treatment through interleukin-10 (Il-10) and transforming growth factor β-1 (TGF β-1). METHODS: Thirty male albino rats were used. A single intraarticular (i.a.) injection of 2mg of Monosodium Iodoacetate (MIA) was injected into the knee joint of anesthetized rats...
February 11, 2017: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/28213973/transforming-growth-factor-%C3%AE-induces-bone-marrow-mesenchymal-stem-cell-migration-via-noncanonical-signals-and-n-cadherin
#9
Maria Jose Dubon, Jinyeong Yu, Sanghyuk Choi, Ki-Sook Park
Transforming growth factor-beta (TGF-β) induces the migration and mobilization of bone marrow-derived mesenchymal stem cells (BM-MSCs) to maintain bone homeostasis during bone remodeling and facilitate the repair of peripheral tissues. Although many studies have reported the mechanisms through which TGF-β mediates the migration of various types of cells, including cancer cells, the intrinsic cellular mechanisms underlying cellular migration and mobilization of BM-MSCs mediated by TGF-β are unclear. In this study, we showed that TGF-β activated noncanonical signaling molecules, such as Akt, extracellular signal-regulated kinase 1/2 (ERK1/2), focal adhesion kinase (FAK), and p38, via TGF-β type I receptor in human BM-MSCs and murine BM-MSC-like ST2 cells...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28213468/microencapsulation-of-lefty-secreting-engineered-cells-for-pulmonary-fibrosis-therapy-in-mice
#10
Hongge Ma, Shupei Qiao, Zeli Wang, Shuai Geng, Yufang Zhao, Xiaolu Hou, Weiming Tian, Xiongbiao Chen, Lifen Yao
Idiopathic pulmonary fibrosis (IPF) is a progressive disease that causes unremitting deposition of extracellular matrix proteins, thus resulting in distortion of the pulmonary architecture and impaired gas exchange. Associated with high morbidity and mortality, IPF is generally refractory to current pharmacological therapies. Lefty A, a potent inhibitor of transforming growth factor (TGF)-β signaling, has been shown to have promising antifibrotic ability in vitro for the treatment of renal fibrosis and other potential organ fibroses...
February 17, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28212670/characterization-of-schistosoma-japonicum-cp1412-protein-as-a-novel-member-of-the-ribonuclease-t2-molecule-family-with-immune-regulatory-function
#11
Xue-Dan Ke, Shuang Shen, Li-Jun Song, Chuan-Xin Yu, Mihoko Kikuchi, Kenji Hirayama, Hong Gao, Jie Wang, Xuren Yin, Yuan Yao, Qian Liu, Wei Zhou
BACKGROUND: Schistosome infection typically induces a polarized Th2 type host immune response. As egg antigen molecules play key roles in this immunoregulatory process, clarifying their functions in schistosomiasis would facilitate the development of vaccine and immunotherapeutic methods. Schistosoma japonicum (Sj) CP1412 (GenBank: AY57074.1) has been identified as a new member of the RNase T2 family with immune regulatory functions. METHODS: The expression plasmid Sj CP1412-pET28a was constructed and transformed into bacteria for production of recombinant Sj CP1412 protein (rSj CP1412) via IPTG induction...
February 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28210622/epigenetics-and-signaling-pathways-in-glaucoma
#12
REVIEW
Angela C Gauthier, Ji Liu
Glaucoma is the most common cause of irreversible blindness worldwide. This neurodegenerative disease becomes more prevalent with aging, but predisposing genetic and environmental factors also contribute to increased risk. Emerging evidence now suggests that epigenetics may also be involved, which provides potential new therapeutic targets. These three factors work through several pathways, including TGF-β, MAP kinase, Rho kinase, BDNF, JNK, PI-3/Akt, PTEN, Bcl-2, Caspase, and Calcium-Calpain signaling. Together, these pathways result in the upregulation of proapoptotic gene expression, the downregulation of neuroprotective and prosurvival factors, and the generation of fibrosis at the trabecular meshwork, which may block aqueous humor drainage...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28210081/role-of-lap-cd4-t-cells-in-the-tumor-microenvironment-of-colorectal-cancer
#13
Wu Zhong, Zhi-Yuan Jiang, Lei Zhang, Jia-Hao Huang, Shi-Jun Wang, Cun Liao, Bin Cai, Li-Sheng Chen, Sen Zhang, Yun Guo, Yun-Fei Cao, Feng Gao
AIM: To investigate the abundance and potential functions of LAP(+)CD4(+) T cells in colorectal cancer (CRC). METHODS: Proportions of LAP(+)CD4(+) T cells were examined in peripheral blood and tumor/paratumor tissues of CRC patients and healthy controls using flow cytometry. Expression of phenotypic markers such as forkhead box (Fox)p3, cytotoxic T-lymphocyte-associated protein (CTLA)-4, chemokine CC receptor (CCR)4 and CCR5 was measured using flow cytometry. LAP(-)CD4(+) and LAP(+)CD4(+) T cells were isolated using a magnetic cell-sorting system and cell purity was analyzed by flow cytometry...
January 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28210072/the-parenteral-vitamin-c-improves-sepsis-and-sepsis-induced-multiple-organ-dysfunction-syndrome-via-preventing-cellular-immunosuppression
#14
Yu-Lei Gao, Bin Lu, Jian-Hua Zhai, Yan-Cun Liu, Hai-Xia Qi, Ying Yao, Yan-Fen Chai, Song-Tao Shou
Cellular immunosuppression appears to be involved in sepsis and sepsis-induced multiple organ dysfunction syndrome (MODS). Recent evidence showed that parenteral vitamin C (Vit C) had the ability to attenuate sepsis and sepsis-induced MODS. Herein, we investigated the impact of parenteral Vit C on cellular immunosuppression and the therapeutic value in sepsis. Using cecal ligation and puncture (CLP), sepsis was induced in WT and Gulo(-/-) mice followed with 200 mg/Kg parenteral Vit C administration. The immunologic functions of CD4(+)CD25(+) regulatory T cells (Tregs) and CD4(+)CD25(-) T cells, as well as the organ functions, were determined...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28209993/suppression-of-tak1-pathway-by-shear-stress-counteracts-the-inflammatory-endothelial-cell-phenotype-induced-by-oxidative-stress-and-tgf-%C3%AE-1
#15
Ee Soo Lee, Llorenç Solé Boldo, Bernadette O Fernandez, Martin Feelisch, Martin C Harmsen
Endothelial dysfunction is characterised by aberrant redox signalling and an inflammatory phenotype. Shear stress antagonises endothelial dysfunction by increasing nitric oxide formation, activating anti-inflammatory pathways and suppressing inflammatory pathways. The TAK1 (MAP3K7) is a key mediator of inflammation and non-canonical TGF-β signalling. While the individual roles of TAK1, ERK5 (MAPK7) and TGF-β pathways in endothelial cell regulation are well characterised, an integrative understanding of the orchestration of these pathways and their crosstalk with the redox system under shear stress is lacking...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209981/fibulin-6-regulates-pro-fibrotic-tgf-%C3%AE-responses-in-neonatal-mouse-ventricular-cardiac-fibroblasts
#16
Arpita Chowdhury, Lisa Hasselbach, Frank Echtermeyer, Nidhi Jyotsana, Gregor Theilmeier, Christine Herzog
Fibulin-6, an essential component of extracellular matrix determines the architecture of cellular junctions in tissues undergoing strain. Increased expression and deposition of fibulin-6 facilitates fibroblast migration in response to TGF-β, following myocardial infarction in mouse heart. The underlying mechanism still remains elusive. In conjunction with our previous study, we have now demonstrated that in fibulin-6 knockdown (KD) fibroblasts, not only TGF-β dependent migration, but also stress fiber formation, cellular networking and subsequently fibroblast wound contraction is almost abrogated...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209920/effect-of-captopril-on-radiation-induced-tgf-%C3%AE-1-secretion-in-ea-hy926-human-umbilical-vein-endothelial-cells
#17
Jingni Wei, Hui Xu, Yinyin Liu, Baiyu Li, Fuxiang Zhou
The pathophysiological mechanism involved in the sustained endothelial secretion of cytokines that leads to fibrosis 6-16 months after radiotherapy remains unclear. Angiotensin II (Ang II) is produced by the endothelium in response to stressing stimuli, like radiation, and may induce the synthesis of TGF-β, a profibrotic cytokine. In this study we tested the hypothesis that captopril, an angiotensin-converting enzyme (ACE) inhibitor, inhibits or attenuates radiation-induced endothelial TGF-β1 secretion. The human endothelial hybrid cell line EA...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28209528/decellularized-human-colorectal-cancer-matrices-polarize-macrophages-towards-an-anti-inflammatory-phenotype-promoting-cancer-cell-invasion-via-ccl18
#18
M L Pinto, E Rios, A C Silva, S C Neves, H R Caires, A T Pinto, C Durães, F A Carvalho, A P Cardoso, N C Santos, C C Barrias, D S Nascimento, P Pinto-do-Ó, M A Barbosa, F Carneiro, M J Oliveira
Macrophages are frequently identified in solid tumors, playing important roles in cancer progression. Their remarkable plasticity makes them very sensitive to environmental factors, including the extracellular matrix (ECM). In the present work, we investigated the impact of human colorectal tumor matrices on macrophage polarization and on macrophage-mediated cancer cell invasion. Accordingly, we developed an innovative 3D-organotypic model, based on the decellularization of normal and tumor tissues derived from colorectal cancer patients' surgical resections...
February 5, 2017: Biomaterials
https://www.readbyqxmd.com/read/28209294/sulfated-glycosaminoglycans-and-non-classically-secreted-proteins-basic-fgf-and-epimorphin-coordinately-regulate-tgf-%C3%AE-induced-cell-behaviors-of-human-scar-dermal-fibroblasts
#19
Tomoatsu Horigome, Shinya Takumi, Kota Shirai, Takumi Kido, Natsumi Hagiwara-Chatani, Ayumi Nakashima, Naoki Adachi, Hiroko Yano, Yohei Hirai
BACKGROUND: Upon skin injuries, dermal fibroblasts actively produce transforming growth factor-β (TGF-β), which leads to the formation of α-smooth muscle actin (αSMA)-positive granulation tissues. The hyperplasia or incomplete regression of these tissues subsequently causes scar formation in the skin, where sulfated glycosaminoglycans (GAGs), side chains of unique proteoglycans, are supposed to play important roles. OBJECTIVE: The aim of this study is to clarify the effects of sulfated GAGs on dermal cell behaviors triggered by the TGF-β signaling, along with its possible regulators basic fibroblast growth factor (bFGF) and cell surface epimorphin...
February 3, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28207769/role-of-glycogen-synthase-kinase-3%C3%AE-and-ppar-%C3%AE-on-epithelial-to-mesenchymal-transition-in-dss-induced-colorectal-fibrosis
#20
Jacopo Di Gregorio, Roberta Sferra, Silvia Speca, Antonella Vetuschi, Caroline Dubuquoy, Pierre Desreumaux, Simona Pompili, Loredana Cristiano, Eugenio Gaudio, Vincenzo Flati, Giovanni Latella
BACKGROUND: Intestinal fibrosis is characterized by abnormal production and deposition of extracellular matrix (ECM) proteins by activated myofibroblasts. The main progenitor cells of activated myofibroblasts are the fibroblasts and the epithelial cells, the latter through the epithelial-mesenchymal transition (EMT). AIM: To evaluate the action of the new PPAR-γ modulator, GED-0507-34 Levo (GED) on the expression of EMT associated and regulatory proteins such as TGF-β, Smad3, E-cadherin, Snail, ZEB1, β-catenin, and GSK-3β, in a mouse model of DSS-induced intestinal fibrosis...
2017: PloS One
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