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https://www.readbyqxmd.com/read/27906600/role-of-polysaccharide-and-lipid-in-lipopolysaccharide-induced-prion-protein-conversion
#1
Carol L Ladner-Keay, Marcia LeVatte, David S Wishart
Conversion of native cellular prion protein (PrP(c)) from an α-helical structure to a toxic and infectious β-sheet structure (PrP(Sc)) is a critical step in the development of prion disease. There are some indications that the formation of PrP(Sc) is preceded by a β-sheet rich PrP (PrP(β)) form which is non-infectious, but is an intermediate in the formation of infectious PrP(Sc). Furthermore the presence of lipid cofactors is thought to be critical in the formation of both intermediate-PrP(β) and lethal, infectious PrP(Sc)...
December 1, 2016: Prion
https://www.readbyqxmd.com/read/27693835/structural-elucidation-of-anti-metastatic-rhamnogalacturonan-ii-from-the-pectinase-digest-of-citrus-peels-citrus-unshiu
#2
Hye-Ryung Park, Su Beom Park, Hee-Do Hong, Hyung Joo Suh, Kwang-Soon Shin
The aim of this study was to characterize a polysaccharide found in citrus peels with an anti-metastatic property. CPE-II was purified by the pectinase digestion of citrus peels. During in vivo lung metastasis of Colon26-M3.1, administration of 10μg of CPE-II per mouse showed 81.3% inhibition of metastasis. CPE-II consists of 15 different monosaccharides and 22 different glycosyl linkages, characteristic of rhamnogalacturonan II (RG-II). The primary structure was elucidated based on sugar composition, methylation analysis, oligosaccharide analysis, and sequencing using GC, GC-MS, LC-MS, and ESI-MS/MS analyses...
September 29, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27626133/4-methoxyphenacyl-assisted-synthesis-of-beta-kdo-glycosides
#3
Marcelina Mazur, Barbara Barycza, Hanitra Andriamboavonjy, Serge Lavoie, Marielle Tamigney Kenfack, Anaïs Laroussarie, Yves Bleriot, Charles Gauthier
3-Deoxy-beta-d-manno-oct-2-ulosonic acid (beta-Kdo) glycosides are mainly found in capsular polysaccharides (CPS) and extracellular exopolysaccharides (EPS) from Gram-negative bacteria. These compounds have profound biological implications in immune response and act as virulence factors. We have developed a novel methodology for the stereoselective synthesis of beta-Kdo glycosides via the use of a 4'-methoxyphenacyl (Phen) auxiliary group at the C1 position of a peracetylated beta-Kdo thioglycoside. Under the promotion of NIS/AgOTf in acetonitrile, a series of Kdo glycosides was synthesized in good yield and beta-selectivity while minimizing the formation of undesirable glycals...
September 14, 2016: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/27613802/the-lipopolysaccharide-of-the-crop-pathogen-xanthomonas-translucens-pv-translucens-chemical-characterization-and-determination-of-signaling-events-in-plant-cells
#4
Tim Steffens, Katarzyna Duda, Buko Lindner, Frank-Jörg Vorhölter, Hanna Bednarz, Karsten Niehaus, Otto Holst
Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2-ulopyranosonic acid (Kdo) and fatty acids (10:0, 11:0, 11:0(3-OH) i/a, 11:0(3-OH), 12:0(3-OH) i/a, 12:0(3-OH), 12:0, 13:0(3-OH) i, 13:0(3-OH) a, 13:0(3-OH), 14:0(3-OH) i/a, 14:0(3-OH) and 16:0)...
September 9, 2016: Glycobiology
https://www.readbyqxmd.com/read/27577896/structure-and-anticancer-activity-of-sulfated-o-polysaccharide-from-marine-bacterium-cobetia-litoralis-kmm-3880-t
#5
Maxim S Kokoulin, Alexandra S Kuzmich, Anatoly I Kalinovsky, Svetlana V Tomshich, Lyudmila A Romanenko, Valery V Mikhailov, Nadezhda A Komandrova
We presented the structure of the polysaccharide moiety and anticancer activity in vitro of the sulfated lipopolysaccharide isolated from the marine bacterium Cobetia litoralis KMM 3880(T). The structure of O-polysaccharide was investigated by chemical methods along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was built up of branched trisaccharide repeating units consist of D-glucose (D-Glcр), D-mannose (D-Manр) and sulfated 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo5S): →7-β-Kdoр4Ac5S-(2→4)-[β-d-Glcp-(1→2)-]-β-d-Manр6Ac-1→...
December 10, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27539854/characterization-of-a-novel-d-glycero-d-talo-oct-2-ulosonic-acid-substituted-lipid-a-moiety-in-the-lipopolysaccharide-produced-by-the-acetic-acid-bacterium-acetobacter-pasteurianus-nbrc-3283
#6
Masahito Hashimoto, Mami Ozono, Maiko Furuyashiki, Risako Baba, Shuhei Hashiguchi, Yasuo Suda, Koichi Fukase, Yukari Fujimoto
Acetobacter pasteurianus is an aerobic Gram-negative rod that is used in the fermentation process used to produce the traditional Japanese black rice vinegar kurozu. Previously, we found that a hydrophobic fraction derived from kurozu stimulates Toll-like receptors to produce cytokines. LPSs, particularly LPS from A. pasteurianus, are strong candidates for the immunostimulatory component of kurozu. The LPS of A. pasteurianus remains stable in acidic conditions during the 2 years of the abovementioned fermentation process...
September 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27535220/biochemical-characterization-of-bifunctional-3-deoxy-%C3%AE-d-manno-oct-2-ulosonic-acid-%C3%AE-kdo-transferase-kpsc-from-escherichia-coli-involved-in-capsule-biosynthesis
#7
Olga G Ovchinnikova, Liam Doyle, Bo-Shun Huang, Matthew S Kimber, Todd L Lowary, Chris Whitfield
3-Deoxy-d-manno-oct-2-ulosonic acid (Kdo) is an essential component of bacterial lipopolysaccharides, where it provides the linkage between lipid and carbohydrate moieties. In all known LPS structures, Kdo residues possess α-anomeric configurations, and the corresponding inverting α-Kdo transferases are well characterized. Recently, it has been shown that a large group of capsular polysaccharides from Gram-negative bacteria, produced by ATP-binding cassette transporter-dependent pathways, are also attached to a lipid anchor through a conserved Kdo oligosaccharide...
October 7, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27516128/mechanistic-insight-into-heptosyltransferase-inhibition-by-using-kdo-multivalent-glycoclusters
#8
Abdellatif Tikad, Huixiao Fu, Charlotte M Sevrain, Sophie Laurent, Jean-François Nierengarten, Stéphane P Vincent
The synthesis of unprecedented multimeric Kdo glycoclusters based on fullerene and calix[4]arene central scaffolds is reported. The compounds were used to study the mechanism and scope of multivalent glycosyltransferase inhibition. Multimeric mannosides based on porphyrin and pillar[5]arenes were also generated in a controlled manner. Twelve glycoclusters and their monomeric ligands were thus assayed against heptosyltransferase WaaC, which is an important bacterial glycosyltransferase that is involved in lipopolysaccharide biosynthesis...
September 5, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27326857/evidence-suggesting-that-francisella-tularensis-o-antigen-capsule-contains-a-lipid-a-like-molecule-that-is-structurally-distinct-from-the-more-abundant-free-lipid-a
#9
Jason H Barker, Justin W Kaufman, Michael A Apicella, Jerrold P Weiss
Francisella tularensis, the Gram-negative bacterium that causes tularemia, produces a high molecular weight capsule that is immunologically distinct from Francisella lipopolysaccharide but contains the same O-antigen tetrasaccharide. To pursue the possibility that the capsule of Francisella live vaccine strain (LVS) has a structurally unique lipid anchor, we have metabolically labeled Francisella with [14C]acetate to facilitate highly sensitive compositional analysis of capsule-associated lipids. Capsule was purified by two independent methods and yielded similar results...
2016: PloS One
https://www.readbyqxmd.com/read/27308198/scope-and-limitations-of-3-iodo-kdo-fluoride-based-glycosylation-chemistry-using-n-acetyl-glucosamine-acceptors
#10
Barbara Pokorny, Paul Kosma
The ketosidic linkage of 3-deoxy-d-manno-octulosonic acid (Kdo) to lipid A constitutes a general structural feature of the bacterial lipopolysaccharide core. Glycosylation reactions of Kdo donors, however, are challenging due to the absence of a directing group at C-3 and elimination reactions resulting in low yields and anomeric selectivities of the glycosides. While 3-iodo-Kdo fluoride donors showed excellent glycosyl donor properties for the assembly of Kdo oligomers, glycosylation of N-acetyl-glucosamine derivatives was not straightforward...
December 2015: ChemistryOpen
https://www.readbyqxmd.com/read/27274586/progress-in-kdo-glycoside-chemistry
#11
Paul Kosma
Glycosylation chemistry of 3-deoxy-D-manno-oct-2-ulosonic acid units has been considerably developed within the last decade. This review covers major achievements with respect to improved yields and anomeric selectivity as well as suppression of the elimination side reaction via selection of dedicated protecting groups and appropriate activation of the anomeric center.
May 18, 2016: Tetrahedron Letters
https://www.readbyqxmd.com/read/27199480/bacterial-%C3%AE-kdo-glycosyltransferases-represent-a-new-glycosyltransferase-family-gt99
#12
Olga G Ovchinnikova, Evan Mallette, Akihiko Koizumi, Todd L Lowary, Matthew S Kimber, Chris Whitfield
Kdo (3-deoxy-d-manno-oct-2-ulosonic acid) is an eight-carbon sugar mostly confined to Gram-negative bacteria. It is often involved in attaching surface polysaccharides to their lipid anchors. α-Kdo provides a bridge between lipid A and the core oligosaccharide in all bacterial LPSs, whereas an oligosaccharide of β-Kdo residues links "group 2" capsular polysaccharides to (lyso)phosphatidylglycerol. β-Kdo is also found in a small number of other bacterial polysaccharides. The structure and function of the prototypical cytidine monophosphate-Kdo-dependent α-Kdo glycosyltransferase from LPS assembly is well characterized...
May 31, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27173798/enzymatic-synthesis-of-3-deoxy-d-manno-octulosonic-acid-kdo-and-its-application-for-lps-assembly
#13
Liuqing Wen, Yuan Zheng, Tiehai Li, Peng George Wang
The studies of 3-deoxy-d-manno-octulosonic acid (KDO) have been hindered due to its limited availability. Herein, an efficient enzymatic system for the facile synthesis of KDO from easy-to-get starting materials is described. In this one-pot three-enzyme (OPME) system, d-ribulose 5-phosphate, which was prepared from d-xylose, was employed as starting materials. The reaction process involves the isomerization of d-ribulose 5-phosphate to d-arabinose 5-phosphate catalyzed by d-arabinose 5-phosphate isomerase (KdsD), the aldol condensation of d-arabinose 5-phosphate and phosphoenolpyruvate (PEP) catalyzed by KDO 8-phosphate synthetase (KdsA), and the hydrolysis of KDO-8-phosphate catalyzed by KDO 8-phosphate phosphatase (KdsC)...
June 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27085741/fractionation-and-analysis-of-lipopolysaccharide-derived-oligosaccharides-by-zwitterionic-type-hydrophilic-interaction-liquid-chromatography-coupled-with-electrospray-ionisation-mass-spectrometry
#14
Aleksandra Man-Kupisinska, Ewelina Bobko, Tomasz K Gozdziewicz, Anna Maciejewska, Wojciech Jachymek, Czeslaw Lugowski, Jolanta Lukasiewicz
Lipopolysaccharide (LPS, endotoxin) is a main surface antigen and virulence factor of Gram-negative bacteria. Regardless of the source of LPS, this molecule, isolated from the smooth forms of bacteria, is characterised by a general structural layout encompassing three regions: (i) an O-specific polysaccharide (O-PS) - a polymer of repeating oligosaccharide units, (ii) core oligosaccharide (OS), and (iii) the lipid A anchoring LPS in the outer membrane of the cell envelope of Gram-negative bacteria. Structural analysis usually requires degradation of LPS and further efficient separation of various poly- and oligosaccharide glycoforms...
June 2, 2016: Carbohydrate Research
https://www.readbyqxmd.com/read/27045037/genetic-and-molecular-basis-of-kingella-kingae-encapsulation
#15
Kimberly F Starr, Eric A Porsch, Patrick C Seed, Joseph W St Geme
Kingella kingae is a common cause of invasive disease in young children and was recently found to produce a polysaccharide capsule containing N-acetylgalactosamine (GalNAc) and β-3-deoxy-d-manno-octulosonic acid (βKdo). Given the role of capsules as important virulence factors and effective vaccine antigens, we set out to determine the genetic determinants of K. kingae encapsulation. Using a transposon library and a screen for nonencapsulated mutants, we identified the previously identified ctrABCD (ABC transporter) operon, a lipA (kpsC)-like gene, a lipB (kpsS)-like gene, and a putative glycosyltransferase gene designated csaA (capsule synthesis type a gene A)...
June 2016: Infection and Immunity
https://www.readbyqxmd.com/read/26953329/crystal-structure-of-a-complex-of-surfactant-protein-d-sp-d-and-haemophilus-influenzae-lipopolysaccharide-reveals-shielding-of-core-structures-in-sp-d-resistant-strains
#16
Howard W Clark, Rose-Marie Mackay, Mary E Deadman, Derek W Hood, Jens Madsen, E Richard Moxon, J Paul Townsend, Kenneth B M Reid, Abdul Ahmed, Amy J Shaw, Trevor J Greenhough, Annette K Shrive
The carbohydrate recognition domains (CRDs) of lung collectin surfactant protein D (SP-D) recognize sugar patterns on the surface of lung pathogens and promote phagocytosis. Using Haemophilus influenzae Eagan strains expressing well-characterized lipopolysaccharide (LPS) surface structures of various levels of complexity, we show that bacterial recognition and binding by SP-D is inversely related to LPS chain extent and complexity. The crystal structure of a biologically active recombinant trimeric SP-D CRD complexed with a delipidated Eagan 4A LPS suggests that efficient LPS recognition by SP-D requires multiple binding interactions utilizing the three major ligand-binding determinants in the SP-D binding pocket, with Ca-dependent binding of inner-core heptose accompanied by interaction of anhydro-Kdo (4,7-anhydro-3-deoxy-d-manno-oct-2-ulosonic acid) with Arg343 and Asp325...
May 2016: Infection and Immunity
https://www.readbyqxmd.com/read/26934919/the-klebsiella-pneumoniae-o12-atp-binding-cassette-abc-transporter-recognizes-the-terminal-residue-of-its-o-antigen-polysaccharide-substrate
#17
Evan Mann, Evan Mallette, Bradley R Clarke, Matthew S Kimber, Chris Whitfield
Export of the Escherichia coli serotype O9a O-antigenic polysaccharides (O-PS) involves an ATP-binding cassette (ABC) transporter. The process requires a non-reducing terminal residue, which is recognized by a carbohydrate-binding module (CBM) appended to the C terminus of the nucleotide-binding domain of the transporter. Here, we investigate the process in Klebsiella pneumoniae serotype O12 (and Raoultella terrigena ATCC 33257). The O12 polysaccharide is terminated at the non-reducing end by a β-linked 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residue...
April 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26934862/lipopolysaccharide-structure-and-biosynthesis-in-helicobacter-pylori
#18
REVIEW
Hong Li, Tingting Liao, Aleksandra W Debowski, Hong Tang, Hans-Olof Nilsson, Keith A Stubbs, Barry J Marshall, Mohammed Benghezal
This review covers the current knowledge and gaps in Helicobacter pylori lipopolysaccharide (LPS) structure and biosynthesis. H. pylori is a Gram-negative bacterium which colonizes the luminal surface of the human gastric epithelium. Both a constitutive alteration of the lipid A preventing TLR4 elicitation and host mimicry of the Lewis antigen decorated O-antigen of H. pylori LPS promote immune escape and chronic infection. To date, the complete structure of H. pylori LPS is not available, and the proposed model is a linear arrangement composed of the inner core defined as the hexa-saccharide (Kdo-LD-Hep-LD-Hep-DD-Hep-Gal-Glc), the outer core composed of a conserved trisaccharide (-GlcNAc-Fuc-DD-Hep-) linked to the third heptose of the inner core, the glucan, the heptan and a variable O-antigen, generally consisting of a poly-LacNAc decorated with Lewis antigens...
December 2016: Helicobacter
https://www.readbyqxmd.com/read/26929331/crosstalk-between-the-lipopolysaccharide-and-phospholipid-pathways-during-outer-membrane-biogenesis-in-escherichia-coli
#19
Akintunde Emiola, Steven S Andrews, Carolin Heller, John George
The outer membrane of gram-negative bacteria is composed of phospholipids in the inner leaflet and lipopolysaccharides (LPS) in the outer leaflet. LPS is an endotoxin that elicits a strong immune response from humans, and its biosynthesis is in part regulated via degradation of LpxC (EC 3.5.1.108) and WaaA (EC 2.4.99.12/13) enzymes by the protease FtsH (EC 3.4.24.-). Because the synthetic pathways for both molecules are complex, in addition to being produced in strict ratios, we developed a computational model to interrogate the regulatory mechanisms involved...
March 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26903955/microevolution-analysis-of-bacillus-coahuilensis-unveils-differences-in-phosphorus-acquisition-strategies-and-their-regulation
#20
Zulema Gómez-Lunar, Ismael Hernández-González, María-Dolores Rodríguez-Torres, Valeria Souza, Gabriela Olmedo-Álvarez
Bacterial genomes undergo numerous events of gene losses and gains that generate genome variability among strains of the same species (microevolution). Our aim was to compare the genomes and relevant phenotypes of three Bacillus coahuilensis strains from two oligotrophic hydrological systems in the Cuatro Ciénegas Basin (México), to unveil the environmental challenges that this species cope with, and the microevolutionary differences in these genotypes. Since the strains were isolated from a low P environment, we placed emphasis on the search of different phosphorus acquisition strategies...
2016: Frontiers in Microbiology
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