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https://www.readbyqxmd.com/read/27925666/enriching-s-4-u-rna-using-methane-thiosulfonate-mts-chemistry
#1
Erin E Duffy, Matthew D Simon
Metabolic labeling of cellular RNA is a useful approach to study RNA biology. 4-Thiouridine (s(4) U) is a convenient nucleoside for metabolic labeling because it is cell permeable and is incorporated into newly transcribed RNA, and the sulfur moiety provides a handle for biochemical purification. However, a critical step in the purification of s(4) U-RNA is the efficiency of the chemistry used to enrich s(4) U-RNA. Here, we present a protocol for s(4) U-RNA enrichment that includes efficient and reversible covalent chemistry to biotinylate s(4) U-RNA using the activated disulfide methane thiosulfonate conjugated to biotin (MTS-biotin), followed by enrichment on streptavidin beads...
December 7, 2016: Current Protocols in Chemical Biology
https://www.readbyqxmd.com/read/27914322/association-between-pigs-with-high-caecal-salmonella-loads-and-carcass-contamination
#2
M Pesciaroli, L Cucco, S De Luca, F R Massacci, C Maresca, L Medici, M Paniccià, E Scoccia, M Staffolani, G Pezzotti, C F Magistrali
Contaminated pork is a significant source of foodborne Salmonella infections. Pork is contaminated at the slaughterhouse; however, the mechanisms driving Salmonella contamination of carcasses are still poorly understood. The aim of this study was to investigate whether the amount of Salmonella carried by slaughtered pigs in their guts has an influence on carcass contamination. On that account, we tested whether the number of carcasses contaminated during a slaughter day was associated with the prevalence of highly contaminated pigs (HCP: Salmonella caecal loads ≥3log/g), or with the prevalence of pigs that simply carry Salmonella spp...
November 23, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27909885/kinematics-and-muscle-activation-patterns-during-a-maximal-voluntary-rate-activity-in-healthy-elderly-and-young-adults
#3
Eva Chadnova, Nancy St-Onge, Richard Courtemanche, Robert D Kilgour
BACKGROUND: Maximal voluntary rate (MVR) performance tasks can provide important age-related information to the limiting factors associated with movement and the development of fatigue. AIM: To determine whether kinematic and muscle activation patterns during an MVR task differ between young and older adults. METHODS: We continuously measured frequency, amplitude, peak velocity, index of co-contraction and median frequencies of the index finger flexors and extensors during a 20-s MVR task in 10 young and 10 older subjects...
December 1, 2016: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/27906285/highly-alignment-tolerant-and-high-sensitivity-100gb-s-4-%C3%A3-25gb-s-apd-rosa-with-a-thin-film-filter-based-de-multiplexer
#4
Joon Young Huh, Sae-Kyoung Kang, Jie Hyun Lee, Joon Ki Lee, SunMe Kim
We investigate and demonstrate a 100-Gb/s (4x25-Gb/s) receiver optical sub-assembly (ROSA) based on avalanche photodiodes and a thin-film filter-based de-multiplexer. The overall alignment tolerances of the ROSA are relaxed to have larger than ± 25 μm by improving optical coupling structure. The receiver sensitivity of each lane is also measured to be less than -22.2 dBm, a record minimum to our knowledge, at the bit error ratio of 10<sup>-12</sup> for 25.78-Gb/s NRZ signal.
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27899354/collagenous-ultrastructure-of-the-discoid-meniscus-a-transmission-electron-microscopy-study
#5
Young-Hee Choi, Young-Jin Seo, Jong Mun Ha, Koo Hyun Jung, Jeehyoung Kim, Si Young Song
BACKGROUND: The collagen ultrastructure of the discoid lateral meniscus (DLM) has not been precisely defined. PURPOSE: To investigate the ultrastructure of the DLM, focusing on its collagen fibers, and to compare the collagen net architecture between intact and torn DLMs using the Collagen Meniscal Architecture (CMA) scoring system. STUDY DESIGN: Descriptive laboratory study. METHODS: Thirty specimens were taken from 30 patients with a diagnosis of a complete DLM using a 1-piece technique...
November 29, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27868416/-comparison-of-fibrotouch-and-acoustic-radiation-force-impulse-in-diagnosis-of-liver-fibrosis-in-patients-with-chronic-hepatitis-b
#6
Fang Liu, Lin Wei, Shanshan Wang, Bin Huang
Objective: To compare transient elastorgaphy (FibroTouch) and acoustic radiation force impulse (ARFI) in diagnosis of liver fibrosis in patients with chronic hepatitis B. Methods: One hundred and forty five patients with chronic hepatitis B underwent FibroTouch and ARFI examinations in Xixi Hospital of Hangzhou from January to November 2015. The liver stiffness (LSM) was detected by FibroTouch and the liver shear wave velocity (VTQ) was detected by ARFI; liver biopsy was performed in all patients. With biopsy results as gold standards, the diagnostic values of FibroTouch and ARFI for liver fibrosis were analyzed with Spearman correlation analysis and receiver operating characteristic (ROC) curve...
May 25, 2016: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/27834576/medicare-advantage-issues-insights-and-implications-for-the-future
#7
Paul Cotton, Joseph P Newhouse, Kevin G Volpp, A Mark Fendrick, Susan Lynne Oesterle, Pat Oungpasuk, Ruchi Aggarwal, Gail Wilensky, Kathleen Sebelius
Medicare Advantage: Issues, Insights, and Implications for the Future Paul Cotton, Joseph P. Newhouse, PhD, Kevin G. Volpp, MD, PhD, A. Mark Fendrick, MD, Susan Lynne Oesterle, Pat Oungpasuk, Ruchi Aggarwal, Gail Wilensky, PhD, and Kathleen Sebelius Editorial    S-2 D.B. Nash, and A.Y. Schwartz The History, Impact, and Future of the Medicare Advantage Star Ratings System    S-3 P. Cotton Medicare Advantage and Traditional Fee-For-Service Medicare    S-4 J.P. Newhouse Behavioral Economics: Key to Effective Care Management Programs for Patients, Payers, and Providers    S-5 K...
November 2016: Population Health Management
https://www.readbyqxmd.com/read/27827494/solvent-induced-structural-diversity-in-tetranuclear-ni-ii-schiff-base-complexes-the-first-ni4-single-molecule-magnet-with-a-defective-dicubane-like-topology
#8
Radovan Herchel, Ivan Nemec, Marek Machata, Zdeněk Trávníček
Two tetranuclear Ni(II) complexes, namely [Ni4(L)4(CH3OH)3(H2O)]·CH3OH (1) and (Pr3NH)2[Ni4(L)4(CH3COO)2] (2, Pr3N = tripropylamine), were synthesized from a tridentate Schiff base ligand H2L (2-[(E)-(2-hydroxybenzylidene)amino]phenol) and Ni(CH3COO)2·4H2O, using different solvents and their ratios (CH3OH and/or CH2Cl2). The prepared Ni4 complexes are of different structural types, involving an Ni4O4 cubane-like core (1) and Ni4O6 defective dicubane-like core (2), with all the Ni atoms hexacoordinated. The complexes were characterized by elemental analysis, FT-IR spectroscopy, variable temperature and field magnetic measurements, and single crystal X-ray analysis...
November 9, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27812898/effective-biotransformation-of-ethyl-4-chloro-3-oxobutanoate-into-ethyl-s-4-chloro-3-hydroxybutanoate-by-recombinant-e-coli-cczu-t15-whole-cells-in-chcl-gly-water-media
#9
Yong Dai, Bin Huan, Hai-Sheng Zhang, Yu-Cai He
To increase the biocatalytic activity of Escherichia coli CCZU-T15 whole cells, choline chloride/glycerol ([ChCl][Gly]) was firstly used as biocompatible solvent for the effective biotransformation of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl (S)-4-chloro-3-hydroxybutanoate [(S)-CHBE]. Furthermore, L-glutamine (150 mM) was added into [ChCl][Gly]-water ([ChCl][Gly] 12.5 vol%, pH 6.5) media instead of NAD(+) for increasing the biocatalytic efficiency. To further improve the biosynthesis of (S)-CHBE (>99 % e...
November 3, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27791189/a-3fe-4s-cluster-is-required-for-trna-thiolation-in-archaea-and-eukaryotes
#10
Yuchen Liu, David J Vinyard, Megan E Reesbeck, Tateki Suzuki, Kasidet Manakongtreecheep, Patrick L Holland, Gary W Brudvig, Dieter Söll
The sulfur-containing nucleosides in transfer RNA (tRNAs) are present in all three domains of life; they have critical functions for accurate and efficient translation, such as tRNA structure stabilization and proper codon recognition. The tRNA modification enzymes ThiI (in bacteria and archaea) and Ncs6 (in archaea and eukaryotic cytosols) catalyze the formation of 4-thiouridine (s(4)U) and 2-thiouridine (s(2)U), respectively. The ThiI homologs were proposed to transfer sulfur via cysteine persulfide enzyme adducts, whereas the reaction mechanism of Ncs6 remains unknown...
October 24, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27740942/effect-of-blood-activity-on-dosimetric-calculations-for-radiopharmaceuticals
#11
Alexandra Zvereva, Nina Petoussi-Henss, Wei Bo Li, Helmut Schlattl, Uwe Oeh, Maria Zankl, Frank Philipp Graner, Christoph Hoeschen, Stephan G Nekolla, Katia Parodi, Markus Schwaiger
The objective of this work was to investigate the influence of the definition of blood as a distinct source on organ doses, associated with the administration of a novel radiopharmaceutical for positron emission tomography-computed tomography (PET/CT) imaging-(S)-4-(3-(18)F-fluoropropyl)-L-glutamic acid ((18)F-FSPG). Personalised pharmacokinetic models were constructed based on clinical PET/CT images from five healthy volunteers and blood samples from four of them. Following an identifiability analysis of the developed compartmental models, person-specific model parameters were estimated using the commercial program SAAM II...
October 14, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27729462/association-and-interaction-analysis-of-diabetes-mellitus-and-scn10a-for-cardiovascular-autonomic-neuropathy-in-a-chinese-population
#12
Yubao Lv, Linuo Zhou, Zihui Tang, Jingcheng Dong
BACKGROUND: This study assessed the extent to which diabetes mellitus (DM) and SCN10A (rs7375036) and their interaction impact on cardiovascular autonomic neuropathy (CAN) susceptibility in a Chinese Han sample. METHOD: We performed a study in a cross-sectional dataset that included 419 patients with DM and 1557 controls who were genotyped for the presence of the SCN10A rs7375036 polymorphisms. Genotyping was performed by iPLEX technology. The associations of rs7375036 and DM with CAN was assessed by using univariate and multivariate logistic regression controlling for confounders...
October 11, 2016: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/27717800/effect-of-iron-deficiency-anemia-and-iron-supplementation-on-hba1c-levels-implications-for-diagnosis-of-prediabetes-and-diabetes-mellitus-in-asian-indians
#13
S V Madhu, Abhishek Raj, Stuti Gupta, S Giri, Usha Rusia
BACKGROUND: We investigated the effect of iron deficiency anemia (IDA) on levels of glycated hemoglobin (HbA1c) and to compare its levels before and after iron supplementations. METHODS: Age and sex matched subjects were enrolled and clustered in 2 groups: IDA (n=62) and healthy controls (HC; n=60). HbA1c levels were estimated by HPLC. Hemogram were estimated by hematology analyser. Serum ferritin (ELISA) and other parameters of iron profile were measured by standard guidelines of ICSH...
October 5, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27702481/biosynthesis-of-optically-pure-chiral-alcohols-by-a-substrate-coupled-and-biphasic-system-with-a-short-chain-dehydrogenase-from-streptomyces-griseus
#14
Zhuotao Tan, Hongmin Ma, Qing Li, Lingling Pu, Yang Cao, Xudong Qu, Chenjie Zhu, Hanjie Ying
The increasing demand for biocatalysts in synthesizing enantiomerically pure chiral alcohols results from the outstanding characteristics of enzymes in reaction, economic, ecological issues. Many carbonyl reductases for producing chiral alcohols have been reported but there is still a lack of good catalytic efficacies. Herein, five carbonyl reductases from different Streptomyces were discovered by the strategy of genome mining. These reductases were overexpressed, and we chose SgCR for further study as it owned better enzyme activity...
November 2016: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27692379/measuring-urinary-n-acetyl-s-4-hydroxy-2-methyl-2-buten-1-yl-l-cysteine-ipma3-as-a-potential-biomarker-of-isoprene-exposure
#15
K Udeni Alwis, T Liz Bailey, Dhrusti Patel, Liqun Wang, Benjamin C Blount
Isoprene, the 2-methyl analog of 1,3-butadiene, is identified as a possible human carcinogen by the International Agency for Research on Cancer (IARC). Isoprene is ubiquitous in the environment with numerous natural and anthropogenic sources. Tobacco smoke is the main exogenous source of isoprene exposure in indoor environments. Among smoke constituents, isoprene is thought to contribute significantly to cancer risk; however, no selective urinary biomarkers of isoprene exposure have been identified for humans...
October 19, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27687354/the-influence-of-patient-position-on-withdrawal-force-of-lumbar-epidural-catheters-after-total-knee-arthroplasty-a-randomized-trial
#16
Young Ri Kim, Ji Won Choi, Woo Seog Sim, Chul Joong Lee, Choohoon Chang
STUDY OBJECTIVE: Elderly patients with degenerative knee disease may have accompanying degenerative spine conditions. There are no studies on lumbar epidural catheter withdrawal forces in these patients. The aim of this study was to investigate withdrawal forces and possible associated risk factors in patients undergoing total knee arthroplasty (TKA). DESIGN: Prospective randomized trial. SETTING: Operating room and ward in a university hospital...
November 2016: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/27665272/old-hearts-for-modern-investigations-ct-and-mr-for-archaeological-human-hearts-remains
#17
F Z Mokrane, R Colleter, S Duchesne, P Gerard, F Savall, E Crubezy, C Guilbeau-Frugier, R Moreno, A Sewonu, H Rousseau, N Telmon, F Dedouit
INTRODUCTION: Among 800 burials dated between the 15th and 18th centuries and found in the center of Rennes (Brittany, France), a collection of five heart-shaped lead urns was discovered. This material was studied using classical methods (external study, autopsy and histology), and also modern imaging like computed tomography (CT), magnetic resonance (MR) before and after coronary opacification. The aim of this manuscript is to describe different steps of ancient soft tissues study, especially using imaging techniques...
September 14, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27642853/quantifying-the-high-speed-running-and-sprinting-profiles-of-elite-female-soccer-players-during-competitive-matches-using-an-optical-player-tracking-system
#18
Jocelyn K Mara, Kevin G Thompson, Kate L Pumpa, Stuart Morgan
The aim of this study was to determine the high-speed running and sprinting profiles of elite female soccer players during competitive matches using a new Optical Player Tracking System. Eight stationary video cameras were positioned at vantage points surrounding the soccer field so that when each camera view was combined the entire field could be viewed simultaneously. Following each match, an optical player tracking system detected the coordinates (x,y) of each player for every video frame. Algorithms applied to the x and y coordinates were used to determine activity variables for twelve elite female players across seven competitive matches...
September 6, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27628782/comparison-in-toxicity-and-solubilizing-capacity-of-hydroxypropyl-%C3%AE-cyclodextrin-with-different-degree-of-substitution
#19
Pengyu Li, Jia Song, Xiaomin Ni, Qin Guo, Hui Wen, Qiuyun Zhou, Yuanna Shen, Yijun Huang, Pengxin Qiu, Suizhen Lin, Haiyan Hu
Hydroxypropyl-β-cyclodextrin (HP-β-CD) has been widely used as an effective solubilizing agent in pharmaceutical industry for many years. However, the effect of degree of substitution (D.S.) of HP-β-CD on solubilizing capacity and toxicity has not been concerned. In this study, solubilizing capacity of HP-β-CDs with three different D.S. (4.55, 6.16 and 7.76) for 16 drugs were measured and their toxicities were compared by a 7-day i.v. administration (q.d.) study in rats. Generally, HP-β-CD with high D...
September 11, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27626445/tetanus-neurotoxin-neutralizing-antibodies-screened-from-a-human-immune-scfv-antibody-phage-display-library
#20
Han Wang, Rui Yu, Ting Fang, Ting Yu, Xiangyang Chi, Xiaopeng Zhang, Shuling Liu, Ling Fu, Changming Yu, Wei Chen
Tetanus neurotoxin (TeNT) produced by Clostridium tetani is one of the most poisonous protein substances. Neutralizing antibodies against TeNT can effectively prevent and cure toxicosis. Using purified Hc fragments of TeNT (TeNT-Hc) as an antigen, three specific neutralizing antibody clones recognizing different epitopes were selected from a human immune scFv antibody phage display library. The three antibodies (2-7G, 2-2D, and S-4-7H) can effectively inhibit the binding between TeNT-Hc and differentiated PC-12 cells in vitro...
2016: Toxins
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