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https://www.readbyqxmd.com/read/28619586/determination-of-acidity-constants-and-prediction-of-electrophoretic-separation-of-amyloid-beta-peptides
#1
Roger Peró-Gascón, Fernando Benavente, José Barbosa, Victoria Sanz-Nebot
In this paper we describe a strategy to estimate by CE the acidity constants (pKa) of complex polyprotic peptides from their building peptide fragments. CE has been used for the determination of the pKas of five short polyprotic peptides that cover all the sequence of amyloid beta (Aβ) peptides 1-40 and 1-42 (Aβ fragments 1-15, 10-20, 20-29, 25-35 and 33-42). First, the electrophoretic mobility (me) was measured as a function of pH of the background electrolyte (BGE) in the pH range 2-12 (bare fused silica capillary, I=25mM and T=25°C)...
June 3, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28613352/comparison-of-ranibizumab-and-bevacizumab-for-macular-edema-secondary-to-retinal-vein-occlusions-in-routine-clinical-practice
#2
Mehnaz Khan, Karen M Wai, Fabiana Q Silva, Sunil Srivastava, Justis P Ehlers, Aleksandra Rachitskaya, Amy Babiuch, Ryan Deasy, Peter K Kaiser, Andrew P Schachat, Alex Yuan, Rishi P Singh
BACKGROUND AND OBJECTIVE: To determine outcomes of intravitreal ranibizumab (IVR) (Lucentis; Genentech, South San Francisco, CA) versus bevacizumab (IVB) (Avastin; Genentech, South San Francisco, CA) for treatment of macular edema (ME) secondary to retinal vein occlusion (RVO) in routine clinical practice. PATIENTS AND METHODS: A retrospective study identified treatment-naïve patients with ME secondary to RVO where treatment with either IVB or IVR was initiated...
June 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28612170/emended-description-of-the-family-chromatiaceae-phylogenetic-analyses-of-the-genera-alishewanella-rheinheimera-and-arsukibacterium-transfer-of-rheinheimera-longhuensis-lh2-2-t-to-the-genus-alishewanella-and-description-of-alishewanella-alkalitolerans-sp-nov
#3
Shivaji Sisinthy, Dwaipayan Chakraborty, Harikrishna Adicherla, Sathyanarayana Reddy Gundlapally
Phylogenetic analyses were performed for members of the family Chromatiaceae, signature nucleotides deduced and the genus Alishewanella transferred to Chromatiaceae. Phylogenetic analyses were executed for the genera Alishewanella, Arsukibacterium and Rheinheimera and the genus Rheinheimera is proposed to be split, with the creation of the Pararheinheimera gen. nov. Furthermore, the species Rheinheimera longhuensis, is transferred to the genus Alishewanella as Alishewanella longhuensis comb. nov. Besides, the genera Alishewanella and Rheinheimera are also emended...
June 13, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28599321/first-detection-of-foot-and-mouth-disease-virus-o-ind-2001d-in-vietnam
#4
Le T Vu, Ngo T Long, Barbara Brito, Carolina Stenfeldt, Nguyen T Phuong, Bui H Hoang, Steven J Pauszek, Ethan J Hartwig, George R Smoliga, Pham P Vu, Le T V Quang, Vo V Hung, Nguyen D Tho, Pham V Dong, Phan Q Minh, Miranda Bertram, Ian H Fish, Luis L Rodriguez, Do H Dung, Jonathan Arzt
In recent years, foot-and-mouth disease virus (FMDV) serotype O, topotype Middle East-South Asia (ME-SA), lineage Ind-2001d has spread from the Indian subcontinent to the Middle East, North Africa, and Southeast Asia. In the current report, we describe the first detection of this lineage in Vietnam in May, 2015 in Đắk Nông province. Three subsequent outbreaks caused by genetically related viruses occurred between May-October, 2015 after which the virus was not detected in clinical outbreaks for at least 15 subsequent months...
2017: PloS One
https://www.readbyqxmd.com/read/28589290/validation-of-a-single-summary-score-for-the-prolapse-incontinence-sexual-questionnaire-iuga-revised-pisq-ir
#5
Melissa L Constantine, Rachel N Pauls, Rebecca R Rogers, Todd H Rockwood
INTRODUCTION AND HYPOTHESIS: The Prolapse/Incontinence Sexual Questionnaire-International Urogynecology Association (IUGA) Revised (PISQ-IR) measures sexual function in women with pelvic floor disorders (PFDs) yet is unwieldy, with six individual subscale scores for sexually active women and four for women who are not. We hypothesized that a valid and responsive summary score could be created for the PISQ-IR. METHODS: Item response data from participating women who completed a revised version of the PISQ-IR at three clinical sites were used to generate item weights using a magnitude estimation (ME) and Q-sort (Q) approaches...
June 6, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28380514/effects-of-different-feeding-levels-during-three-short-periods-of-gestation-on-gilt-and-litter-performance-nutrient-digestibility-and-energy-homeostasis-in-gilts
#6
P Ren, X J Yang, S Q Cui, J S Kim, D Menon, S K Baidoo
The present study investigated the effects of different feeding levels during 3 short periods of gestation on gilt and litter performance, apparent total tract digestibility (ATTD) of energy and nutrients, and energy homeostasis in gilts. A total of 18 gilts were allotted to 1 of 3 dietary treatments using a completely randomized design. All gilts were fed 1 common corn-soybean meal-based diet with the amount of 1.0 × maintenance energy intake (100 × BW (BW) kcal ME/d) throughout gestation except 3 periods of 7 d when dietary treatments were imposed on d 27, d 55, and d 83 of gestation...
March 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28258075/a-retrospective-study-of-the-influence-of-the-vitreomacular-interface-on-macular-oedema-secondary-to-retinal-vein-occlusion
#7
Rishi P Singh, Karishma A Habbu, Rumneek Bedi, Fabiana Q Silva, Justis P Ehlers, Andrew P Schachat, Jonathan E Sears, Sunil K Srivastava, Peter K Kaiser, Alex Yuan
AIMS: To compare anti-vascular endothelial growth factor (VEGF) treatment outcomes for macular oedema (ME) secondary to retinal vein occlusion (RVO) based on vitreoretinal interface (VRI) status. METHODS: This retrospective case series includes treatment-naive eyes diagnosed with RVO and treated with anti-VEGF injections. Eyes were stratified based on international VRI classification schema at baseline into three groups-vitreomacular traction (group A), no posterior vitreous detachment (PVD) (group B) and PVD without vitreomacular attachment (group C)...
March 3, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28156575/using-a-values-assessment-as-a-bridge-to-advance-care-planning-results-of-1286-patient-reports
#8
Cynthia Taniguchi, Kathryn J Eagye, Susan Ash-Lee, Angela Kalisiak, Sabrina Q Mikan, Nicole Hartung
12 Background: Recent studies have shown that formal Palliative Care (PC) improves care at the end of life for cancer patients. The role of communication in PC is not defined. Other studies have shown that communication alone can be associated with better end of life outcomes. The US Oncology Network developed and tested a Values Assessment (VA) instrument for routinely broaching difficult topics to facilitate more formal advance care planning (ACP). The results of the first 28 months of the program on over 1200 patients are reported here...
October 9, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28059425/metabolic-profiling-of-a-myalgic-encephalomyelitis-chronic-fatigue-syndrome-discovery-cohort-reveals-disturbances-in-fatty-acid-and-lipid-metabolism
#9
Arnaud Germain, David Ruppert, Susan M Levine, Maureen R Hanson
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) remains a continuum spectrum disease without biomarkers or simple objective tests, and therefore relies on a diagnosis from a set of symptoms to link the assortment of brain and body disorders to ME/CFS. Although recent studies show various affected pathways, the underlying basis of ME/CFS has yet to be established. In this pilot study, we compare plasma metabolic signatures in a discovery cohort, 17 patients and 15 matched controls, and explore potential metabolic perturbations as the aftermath of the complex interactions between genes, transcripts and proteins...
January 31, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28046484/we-ab-209-05-development-of-an-ultra-fast-high-quality-whole-breast-radiotherapy-treatment-planning-system
#10
Y Sheng, T Li, S Yoo, F Yin, R Blitzblau, J Horton, M Palta, C Hahn, Y Ge, Q Wu
PURPOSE: To enable near-real-time (<20sec) and interactive planning without compromising quality for whole breast RT treatment planning using tangential fields. METHODS: Whole breast RT plans from 20 patients treated with single energy (SE, 6MV, 10 patients) or mixed energy (ME, 6/15MV, 10 patients) were randomly selected for model training. Additional 20 cases were used as validation cohort. The planning process for a new case consists of three fully automated steps:1...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046153/energy-content-and-amino-acid-digestibility-of-flaxseed-expellers-fed-to-growing-pigs
#11
RANDOMIZED CONTROLLED TRIAL
Y F Chen, F Wu, P L Li, Z Q Lyu, L Liu, M B Lyu, F L Wang, C H Lai
This study was conducted to determine the DE and ME as well as the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA in flaxseed expellers fed to growing pigs. In Exp. 1, the DE and ME were determined using 66 crossbred growing pigs (Duroc × Landrace × Yorkshire; 54.2 ± 2.3 kg BW) fed 1 of 11 diets in a completely randomized design. The diets included a corn-soybean meal basal diet and 10 experimental diets containing 29.16% flaxseed expellers supplemented at the expense of corn and soybean meal...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898961/effects-of-feeding-regimen-on-weight-gain-semen-characteristics-libido-and-lameness-in-170-to-250-kilogram-duroc-boars
#12
RANDOMIZED CONTROLLED TRIAL
C Wang, J L Li, H K Wei, Y F Zhou, J J Tan, H Q Sun, S W Jiang, J Peng
A 2-period field trial was conducted to determine the effects of feeding regimen on weight gain, semen characteristics, libido, and lameness in 170- to 200-kg (period 1) and 200- to 250-kg (period 2) boars. Sixty-one Duroc boars were allotted to 1 of 3 dietary treatments and 15 g/d fish oil was also provided for each experimental boar, to maintain the n-6:n-3 intake ratio at approximately 6.1:1. The energy intakes of the 3 treatment groups were controlled by basing the feed intake on a corn-soybean meal-based diet (3...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27781340/p2x3-receptor-mediated-visceral-hyperalgesia-and-neuronal-sensitization-following-exposure-to-ptsd-like-stress-in-the-dorsal-root-ganglia-of-rats
#13
Y-Q He, X-Q Lang, L Lin, L Ji, X-Y Yuan, Q Chen, Y-M Ran, H-S Chen, L Li, J-M Wang, Z-G Wang, H Gregersen, D-W Zou, H-P Liang, M Yang
BACKGROUND: Patients with posttraumatic stress disorder (PTSD) often share co-morbidity with chronic pain conditions. Recent studies suggest a role of P2X3 receptors and ATP signaling in pain conditions. However, the underlying mechanisms of visceral hyperalgesia following exposure to PTSD-like stress conditions remain unclarified. METHODS: The behavior and hormones relevant for PTSD were studied. Visceromotor responses (VMR) and the abdominal withdrawal reflexes (AWR) to colorectal distention (CRD) were recorded to determine P2X3-receptor-mediated alteration of hyperalgesia following single-prolonged stress (SPS) exposure...
March 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/27676609/value-and-anisotropy-of-the-electron-and-hole-mass-in-pure-wurtzite-inp-nanowires
#14
D Tedeschi, M De Luca, A Granados Del Águila, Q Gao, G Ambrosio, M Capizzi, H H Tan, P C M Christianen, C Jagadish, A Polimeni
The effective mass of electrons and holes in semiconductors is pivotal in determining the dynamics of carriers and their confinement energy in nanostructured materials. Surprisingly, this quantity is still unknown in wurtzite (WZ) nanowires (NWs) made of III-V compounds (e.g., GaAs, InAs, GaP, InP), where the WZ phase has no bulk counterpart. Here, we investigate the magneto-optical properties of InP WZ NWs grown by selective-area epitaxy that provides perfectly ordered NWs featuring high-crystalline quality...
October 12, 2016: Nano Letters
https://www.readbyqxmd.com/read/27465206/a-room-temperature-ultrasensitive-magnetoelectric-susceptometer-for-quantitative-tissue-iron-detection
#15
Hao Xi, Xiaoshi Qian, Meng-Chien Lu, Lei Mei, Sebastian Rupprecht, Qing X Yang, Q M Zhang
Iron is a trace mineral that plays a vital role in the human body. However, absorbing and accumulating excessive iron in body organs (iron overload) can damage or even destroy an organ. Even after many decades of research, progress on the development of noninvasive and low-cost tissue iron detection methods is very limited. Here we report a recent advance in a room-temperature ultrasensitive biomagnetic susceptometer for quantitative tissue iron detection. The biomagnetic susceptometer exploits recent advances in the magnetoelectric (ME) composite sensors that exhibit an ultrahigh AC magnetic sensitivity under the presence of a strong DC magnetic field...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27091812/accelerated-dynamic-mri-using-patch-regularization-for-implicit-motion-compensation
#16
Yasir Q Mohsin, Sajan Goud Lingala, Edward DiBella, Mathews Jacob
PURPOSE: To introduce a fast algorithm for motion-compensated accelerated dynamic MRI. METHODS: An efficient patch smoothness regularization scheme, which implicitly compensates for inter-frame motion, is introduced to recover dynamic MRI data from highly undersampled measurements. The regularization prior is a sum of distances between each rectangular patch in the dataset with other patches in the dataset using a saturating distance metric. Unlike current motion estimation and motion compensation (ME-MC) methods, the proposed scheme does not require reference frames or complex motion models...
March 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/27080489/change-in-choroidal-thickness-and-the-relationship-with-accommodation-following-myopic-excimer-laser-surgery
#17
M Li, H Cheng, Y Yuan, J Wang, Q Chen, R Me, B Ke
PurposeTo investigate early changes in choroidal thickness (CT) and the relationship with accommodation after myopic excimer laser surgery.MethodsWe enrolled the right eye of 70 patients with myopia and without other ophthalmic or systemic diseases who were suitable for myopic excimer laser surgery. The CT was measured at the fovea and at distances of 0.5 and 2.5 mm for the following: nasal; temporal; superior; and inferior to the fovea preoperatively and at 1 month postoperatively. Other data collected included demographic information (age, sex, and refractive error), the amplitude of accommodation (AA), intraocular pressure, axial length, corneal thickness, and surgical parameters...
July 2016: Eye
https://www.readbyqxmd.com/read/27079689/energy-requirements-of-early-weaned-dorper-cross-bred-female-lambs
#18
T Ma, G-S Xu, K-D Deng, S-K Ji, Y Tu, N-F Zhang, Q-Y Diao
The net and metabolizable energy (NE and ME) requirements of Dorper cross-bred female lambs with BWs of 20-35 kg were assessed in a comparative slaughter trial. Thirty-five Dorper × thin-tailed Han cross-bred female lambs weaned at ~50 days of age (20.3 ± 2.15 kg BW) were used. Seven randomly selected lambs were slaughtered at the start of the trial (baseline group). An intermediate group consisting of seven randomly selected lambs fed ad libitum was slaughtered when the lambs reached an average BW of 28...
December 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27038396/polyphenol-rich-fraction-from-larrea-divaricata-and-its-main-flavonoid-quercetin-3-methyl-ether-induce-apoptosis-in-lymphoma-cells-through-nitrosative-stress
#19
Renzo Martino, María Laura Barreiro Arcos, Rosario Alonso, Valeria Sülsen, Graciela Cremaschi, Claudia Anesini
Larrea divaricata is a plant with antiproliferative principles. We have previously identified the flavonoid quercetin-3-methyl ether (Q-3-ME) in an ethyl acetate fraction (EA). Both the extract and Q-3-ME were found to be effective against the EL-4 T lymphoma cell line. However, the mechanism underlying the inhibition of tumor cell proliferation remains to be elucidated. In this work, we analyzed the role of nitric oxide (NO) in the induction of apoptosis mediated by Q-3-ME and EA. Both treatments were able to induce apoptosis in a concentration-dependent and time-dependent manner...
July 2016: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/27016571/metabolic-engineering-of-mortierella-alpina-for-enhanced-arachidonic-acid-production-through-the-nadph-supplying-strategy
#20
Guangfei Hao, Haiqin Chen, Zhennan Gu, Hao Zhang, Wei Chen, Yong Q Chen
UNLABELLED: NADPH is known to be a key cofactor required for fatty acid synthesis and desaturation. Various enzymatic reactions can generate NADPH. To determine the effect of NADPH sources on lipogenesis, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (PGD), isocitrate dehydrogenase (IDH), and malic enzyme (ME) were overexpressed in Mortierella alpina Our results showed that G6PD2 had the most significant effect on fatty acid synthesis, with a 1.7-fold increase in total fatty acid, whereas ME2 was more effective in desaturation, with a 1...
June 1, 2016: Applied and Environmental Microbiology
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