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Perrine Guillaume-Jugnot, Aurélie Daumas, Jérémy Magalon, Elisabeth Jouve, Pierre-Sébastien Nguyen, Romain Truillet, Stéphanie Mallet, Dominique Casanova, Laurent Giraudo, Julie Veran, Françoise Dignat-George, Florence Sabatier, Guy Magalon, Brigitte Granel
OBJECTIVE: Impaired hand function greatly contributes to disability and reduced quality of life in SSc patients. Autologous adipose-derived stromal vascular fraction (ADSVF) is recognized as an easily accessible source of regenerative cells. We reported positive 6-month safety and efficacy results from an open-label clinical trial assessing s.c. injection of autologous ADSVF into the fingers in SSc patients. The objective of this report is to describe the effects at 12 months. METHODS: Twelve females, mean age 54...
February 2016: Rheumatology
Daisuke Ishimura, Naoki Yamamoto, Kaori Tajima, Ayumi Ohno, Yasuhiro Yamamoto, Osuke Washimi, Harumoto Yamada
The incidence of arthritic diseases is rapidly increasing in most advanced countries. Articular cartilage, which is the most important tissue in the joint, consists of chondrocytes and abundant extracellular matrix, including aggrecan, and shows poor self-repair. We studied the potential of stem cells in mouse subcutaneous adipose tissue as a source of cells to regenerate cartilage tissue. Analysis of adipose-derived stromal vascular fraction culture cells (ADSVFs) using mesenchymal stem cell markers showed that CD90-positive cells accounted for 93...
October 2008: Tohoku Journal of Experimental Medicine
Naoki Yamamoto, Hirohiko Akamatsu, Seiji Hasegawa, Takaaki Yamada, Satoru Nakata, Mahito Ohkuma, Ei-Ichi Miyachi, Tohru Marunouchi, Kayoko Matsunaga
BACKGROUND: Embryonic stem (ES) cells, bone marrow, adipose tissue or other genetically modified stem cells are being widely used in basic research in the field of regenerative medicine. However, there is no specific surface antigen that can be used as a marker of multipotent stem cells. OBJECTIVE: We tried to isolate and collect putative multipotent stem cells from mouse subcutaneous adipose tissue using the p75 neurotrophin receptor (p75NTR) as a marker. METHODS: Adipose tissue was processed for immunostaining using antibodies anti-CD90, anti-CD105 and anti-Sca-1 as general mesenchymal stem cell (MSC) markers, and anti-p75NTR, an epithelial stem cell and MSC marker...
October 2007: Journal of Dermatological Science
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