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Periodontal ligament

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https://www.readbyqxmd.com/read/29149622/the-chemokine-receptor-type-4-antagonist-amd3100-interrupts-experimental-tooth-movement-in-rats
#1
Kasumi Hatano, Yuji Ishida, Hiroyuki Yamaguchi, Jun Hosomichi, Jun-Ichi Suzuki, Risa Usumi-Fujita, Yasuhiro Shimizu, Naoki Shibutani, Sawa Kaneko, Takashi Ono
OBJECTIVE: The aim of this study was to clarify the role of the stromal cell-derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4) axis in osteoclast accumulation, and the influence of orthodontic tooth movement (OTM) under mechanical force application to periodontal tissues, by administration of the CXCR4 antagonist AMD3100. DESIGN: The upper right first molar (M1) of rats was moved mesially with a 10-g force titanium-nickel closed coil spring. Rats were treated with phosphate-buffered saline or AMD3100 (5mg/kg), which is a SDF-1 antagonist...
November 11, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29143185/osteogenic-prospective-of-deriving-human-dental-stem-cells-in-collagen-matrix-boost
#2
Tong-Jing Fang, Ding-Han Wang, Chia-Yu Wang, Raju Poongodi, Nien-Hsien Liou, Jiang-Chuan Liu, Ming-Lun Hsu, Po-Da Hong, Shih-Fang Yang, Meng-Lun Liu
Stem cells derived from oral tissue represent a highly attractive alternative source for clinical bone regeneration because they can be collected by non-invasive or minimally invasive procedures. Herein, we describe the human dental stem cells (DSCs) deriving from buccal fat pads (BFP), dental pulp (DP) of impacted teeth, and periodontal ligaments (PDL) to obtain BFPSCs, DPSCs, and PDLSCs, respectively. Cells were purified with selected medium and expanded through passages in stem cell culture medium. Purified cells were characterized for stemness by their growth rate, immunostaining, and multilineage differentiation ability...
November 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29138812/expression-and-regulation-of-the-erk1-2-and-p38-mapk-signaling-pathways-in-periodontal-tissue-remodeling-of-orthodontic-tooth-movement
#3
Liping Jiang, Zhen Tang
The present study aimed to investigate the expression and regulation of extracellular signal‑regulated kinase (ERK)1/2 and p38 mitogen‑activated protein kinase (MAPK) signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Sprague Dawley rats with orthodontic tooth movement were generated. After tension stress for 1, 3, 5, 7 and 14 days, the protein and mRNA expression levels of ERK1/2 and p38 in periodontal tissue were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), respectively...
November 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#4
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29129620/human-adipose-derived-stem-cells-adsc-and-human-periodontal-ligament-stem-cells-pdlsc-as-cellular-substrates-of-a-toxicity-prediction-assay
#5
Natássia Caroline Resende Corrêa, Crisciele Kuligovski, Ariane Caroline Campos Paschoal, Ana Paula Ressetti Abud, Carmen Lucia Kuniyoshi Rebelatto, Lidiane Maria Boldrini Leite, Alexandra Cristina Senegaglia, Bruno Dallagiovanna, Alessandra Melo de Aguiar
With the increasing need to develop in vitro assays to replace animal use, human stem cell-derived methods are emerging and showing outstanding contributions to the toxicological screening of substances. Adult human stem cells such as adipose-derived stem cells (ADSC) and periodontal ligament stem cells (PDLSC) were used as cell substrates for a cytotoxicity assay and toxicity prediction using the neutral red uptake (NRU) assay. First, primary cell cultures from three independent donors, from each tissue source, were characterized as mesenchymal stem cells (MSC) by plastic adherence and appropriate immunophenotype for MSC markers (positive for CD90, CD73, and CD105 and negative for CD11b, CD34, CD45, HLADR, and CD19)...
November 9, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29128350/biomechanical-behavior-of-adhesive-cement-layer-and-periodontal-tissues-on-the-restored-teeth-with-zirconia-rbfdps-using-three-kinds-of-framework-design-3d-fea-study
#6
Ayana Uraba, Reina Nemoto, Kosuke Nozaki, Tasuku Inagaki, Satoshi Omori, Hiroyuki Miura
PURPOSE: To investigate stress and strain concentrations on resin-bonded fixed dental prostheses (RBFDPs) frameworks of different design using finite element analysis. METHODS: A human dry skull was scanned and models were produced using three-dimensional printer. After abutment preparation, three frameworks, including a three-unit RBFDP, and two-unit cantilevered RBFDPs using #21 and #23 for the abutment tooth, were fabricated. Scanned data were subtracted to define boundary of each structure...
November 8, 2017: Journal of Prosthodontic Research
https://www.readbyqxmd.com/read/29126633/mechanical-behavior-of-endodontically-restored-canine-teeth-effects-of-ferrule-post-material-and-shape
#7
Pietro Ausiello, Stefano Ciaramella, Massimo Martorelli, Antonio Lanzotti, Fernando Zarone, David C Watts, Antonio Gloria
OBJECTIVE: To assess the effect of a ferrule design with specific post material-shape combinations on the mechanical behavior of post-restored canine teeth. METHODS: Micro-CT scan images of an intact canine were used to create a 3-D tessellated CAD model, from which the shapes of dentin, pulp and enamel were obtained and geometric models of post-endodontically restored teeth were created. Two types of 15mm post were evaluated: a quartz fiber post with conical-tapered shape, and a carbon (C) fiber post with conical-cylindrical shape...
November 7, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/29125100/effects-of-force-and-inflammatory-factors-on-the-expressions-of-osteogenesis-regulators-in-human-periodontal-ligament-cells
#8
Hua-Jing Zhang, Sheng-Nan Li, Xiao-Nan Xu, Ding Zhang
Objective To observe the effects of force signals and inflammatory cytokines on the expressions of functional proteins during the differentiation of periodontal ligament cells(PDLCs) into osteoclasts. Methods The caries-free premolars that needed to be removed for orthodontic treatment were collected,human periodontal ligament cells were cultured in vitro.Human PDLCs were exposed to inflammatory cytokines including interleukin(IL)-1β,-6,-23,and tumor necrosis factor alpha(TNF-α). Cyclicmechanical tension with a maximum 5% elongation for different durations(0,2,4,8,12,and 24 hours) were applied...
October 30, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29116611/anti-inflammatory-effects-of-low-level-laser-therapy-on-human-periodontal-ligament-cells-in-vitro-study
#9
Ji-Hua Lee, Min-Hsuan Chiang, Ping-Ho Chen, Mei-Ling Ho, Huey-Er Lee, Yan-Hsiung Wang
Periodontal disease is a chronic inflammatory disease that is commonly treated with surgical and nonsurgical techniques. However, both approaches have limitations. Low-level laser therapy (LLLT) has been widely applied in reducing inflammatory reactions, and research indicates that LLLT induces an anti-inflammatory effect that may enhance periodontal disease therapy. The purpose of this study was to investigate the anti-inflammatory effect of LLLT on human periodontal ligament cells (hPDLCs) in an inflammatory environment and aimed to determine the possible mechanism of action...
November 7, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29116140/valid-gene-expression-normalization-by-rt-qpcr-in-studies-on-hpdl-fibroblasts-with-focus-on-orthodontic-tooth-movement-and-periodontitis
#10
Christian Kirschneck, Sarah Batschkus, Peter Proff, Josef Köstler, Gerrit Spanier, Agnes Schröder
Meaningful, reliable and valid mRNA expression analyses by real-time quantitative PCR (RT-qPCR) can only be achieved, if suitable reference genes are chosen for normalization and if appropriate RT-qPCR quality standards are met. Human periodontal ligament (hPDL) fibroblasts play a major mediating role in orthodontic tooth movement and periodontitis. Despite corresponding in-vitro gene expression studies being a focus of interest for many years, no information is available for hPDL fibroblasts on suitable reference genes, which are generally used in RT-qPCR experiments to normalize variability between samples...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29109319/histological-evaluation-of-the-effect-of-platelet-rich-plasma-on-pulp-regeneration-in-nonvital-open-apex-teeth-an-animal-study
#11
Jamileh Ghoddusi, Amir Maghsudlu, Hamid Jafarzadeh, Amirhossein Jafarian, Maryam Forghani
AIM: Platelet-rich plasma (PRP), which is a concentration of growth factors found in platelets, may be a suitable material for pulp regeneration. The aim of this animal study was a histological evaluation of PRP on pulp regeneration in nonvital teeth with immature apices. MATERIALS AND METHODS: A total of 40 premolar dogs' teeth were chosen for this study. After general anesthesia, the teeth were exposed, and subsequently, pulps were removed and the cavities were opened to the oral cavity...
November 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/29106735/human-periodontal-ligament-fibroblasts-synthesize-c-reactive-protein-and-th-related-cytokines-in-response-to-interleukin-il-6-trans-signaling
#12
A Hernández-Caldera, R Vernal, R Paredes, P Veloso-Matta, J Astorga, M Hernández
AIM: To characterize the potential of human periodontal ligament fibroblasts (HPLF) to synthesize CRP and Th-related cytokines in response to IL-6 in periodontal health and apical inflammation. METHODOLOGY: Primary HPLF stimulated with IL-6, soluble(s) IL-6 receptor (R) and controls were assayed for CRP, Th1, Th2, Th17 and Treg-related cytokines by quantitative real time PCR and ELISA, respectively. IL-6R mRNA expression and its soluble protein levels were screened in HPLF cultures, and ex vivo samples of healthy periodontal ligaments (n=5) and apical lesions (n=13)...
November 6, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/29104479/knockdown-of-yes-associated-protein-induces-the-apoptosis-while-inhibits-the-proliferation-of-human-periodontal-ligament-stem-cells-through-crosstalk-between-erk-and-bcl-2-signaling-pathways
#13
Yong Wen, Yawen Ji, Yunpeng Zhang, Baoqi Jiang, Cuizhu Tang, Qi Wang, Xiyan Chen, Linglu Jia, Weiting Gu, Xin Xu
Objective: The purpose of this study was to provide an insight into the biological effects of knockdown Yes-associated protein (YAP) on the proliferation and apoptosis of human periodontal ligament stem cells (h-PDLSCs). Methods: Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression level. Enhanced green fluorescence protein lentiviral vector was constructed to down-regulate YAP in h-PDLSCs. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect the interfering efficiency of YAP expression...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29103443/protein-and-mrna-expressions-of-il-6-and-its-key-signaling-factors-under-orthodontic-forces-in-mice-an-in-vivo-study
#14
Yi Liu, Fang Song, Shu Wu, Shushu He, Mingmei Meng, Chunxiao Lv, Qingqing Yang, Song Chen
INTRODUCTION: The purpose of this study was to investigate the mechanical loading-induced changes in protein and mRNA expressions of interleukin-6 (IL-6) and its key signaling factors glycoprotein 130 (gp130), signal transducer and activator of transcription 3 (STAT3), and the Src homology phosphotyrosine phosphatase (SHP2) at the tension and compression sides of the teeth in mouse models. METHODS: A total of 55 C57B/6 mice (10 weeks old) were divided into 3 groups...
November 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/29103438/numeric-simulation-model-for-long-term-orthodontic-tooth-movement-with-contact-boundary-conditions-using-the-finite-element-method
#15
Ryo Hamanaka, Satoshi Yamaoka, Tuan Nguyen Anh, Jun-Ya Tominaga, Yoshiyuki Koga, Noriaki Yoshida
INTRODUCTION: Although many attempts have been made to simulate orthodontic tooth movement using the finite element method, most were limited to analyses of the initial displacement in the periodontal ligament and were insufficient to evaluate the effect of orthodontic appliances on long-term tooth movement. Numeric simulation of long-term tooth movement was performed in some studies; however, neither the play between the brackets and archwire nor the interproximal contact forces were considered...
November 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/29103437/in-vivo-determination-of-critical-force-levels-using-an-intraoral-electromechanical-device-to-measure-nonpathologic-tooth-mobility
#16
Tim Wucher, Alfred Meyer Dippenaar, Martin Wucher
INTRODUCTION: An electromechanical device was used to experimentally characterize the movement of a single tooth within the periodontal ligament space. The force magnitude leading to the complete compression of the periodontal ligament is considered a critical force and is designated Fc. We investigated the effectiveness of the electromechanical device to repeatedly determine the critical force magnitude Fc. METHODS: The study comprised 12 tests conducted on 11 subjects...
November 2017: American Journal of Orthodontics and Dentofacial Orthopedics
https://www.readbyqxmd.com/read/29093986/static-magnetic-fields-promote-osteoblastic-cementoblastic-differentiation-in-osteoblasts-cementoblasts-and-periodontal-ligament-cells
#17
Eun-Cheol Kim, Jaesuh Park, Il Keun Kwon, Suk-Won Lee, Su-Jung Park, Su-Jin Ahn
Purpose: Although static magnetic fields (SMFs) have been used in dental prostheses and osseointegrated implants, their biological effects on osteoblastic and cementoblastic differentiation in cells involved in periodontal regeneration remain unknown. This study was undertaken to investigate the effects of SMFs (15 mT) on the osteoblastic and cementoblastic differentiation of human osteoblasts, periodontal ligament cells (PDLCs), and cementoblasts, and to explore the possible mechanisms underlying these effects...
October 2017: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/29079935/periodontal-regeneration-induced-by-porous-alpha-tricalcium-phosphate-with-immobilized-basic-fibroblast-growth-factor-in-a-canine-model-of-2-wall-periodontal-defects
#18
Kazuya Matsuse, Yoshiya Hashimoto, Sachiro Kakinoki, Tetsuji Yamaoka, Shosuke Morita
We evaluated the effect of porous alpha-tricalcium phosphate (α-TCP) with immobilized basic fibroblast growth factor (bFGF) on periodontal regeneration in a canine model of 2-wall periodontal defects. Identical bone defects were made in the canine mandible; six defects in each animal were filled with porous α-TCP with bFGF bound via heparin (bFGF group), and the remaining defects were filled with unmodified porous α-TCP (control group). Micro-computed tomography and histological evaluation were performed at 2, 4, and 8 weeks post-implantation...
October 27, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/29076281/human-periodontal-ligament-and-gingiva-derived-mesenchymal-stem-cells-promote-nerve-regeneration-when-encapsulated-in-alginate-hyaluronic-acid-3d-scaffold
#19
Sahar Ansari, Ivana M Diniz, Chider Chen, Patricia Sarrion, Ali Tamayol, Benjamin M Wu, Alireza Moshaverinia
Repair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, it is demonstrated that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs are encapsulated in a 3D scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF)...
October 27, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29074442/conservative-management-of-an-atypical-intra-sinusal-ossifying-fibroma-associated-to-an-aneurysmal-bone-cyst
#20
R Saad, J-C Lutz, S Riehm, L Marcellin, C-I Gros, F Bornert
Ossifying fibroma (OF) is a benign fibro-osseous lesion mainly occurring in young adults and seems to originate from the periodontal ligament. Aneurysmal bone cyst (ABC) is a benign intraosseous lesion characterized by blood-filled spaces of various sizes. These two lesions can specifically affect the jaws and are commonly described in the literature. However, few cases describing an association of OF and ABC have been reported in the literature, especially in the maxillary sinus. We report the case of a 40-year-old male patient affected with an asymptomatic lesion with a dual component of OF and ABC laying in the maxillary sinus...
October 24, 2017: Journal of Stomatology, Oral and Maxillofacial Surgery
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