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Periodontal ligament

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https://www.readbyqxmd.com/read/29783786/graphene-based-nanomaterials-for-tissue-engineering-in-the-dental-field
#1
REVIEW
Riccardo Guazzo, Chiara Gardin, Gloria Bellin, Luca Sbricoli, Letizia Ferroni, Francesco Saverio Ludovichetti, Adriano Piattelli, Iulian Antoniac, Eriberto Bressan, Barbara Zavan
The world of dentistry is approaching graphene-based nanomaterials as substitutes for tissue engineering. Apart from its exceptional mechanical strength, electrical conductivity and thermal stability, graphene and its derivatives can be functionalized with several bioactive molecules. They can also be incorporated into different scaffolds used in regenerative dentistry, generating nanocomposites with improved characteristics. This review presents the state of the art of graphene-based nanomaterial applications in the dental field...
May 20, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29780202/common-occurrence-of-sharpey-s-fibres-in-amphibian-phalanges
#2
Krzysztof Kolenda, Anna Najbar, Beata Rozenblut-Kościsty, Ewa Serwa, Tomasz Skawiński
Sharpey's fibres are known mainly as providing anchorage between tooth and the periodontal ligament but they occur also in other types of bones. In the postcranial skeleton these fibres are usually present at the muscle or tendon attachment sites. They were reported in all major groups of extant vertebrates, as well as in putative lissamphibian ancestors-temnospondyls and lepospondyls. However, it was recently stated that their presence was very rarely described in extant amphibians. In limbs, they were reported predominantly from proximal bones...
2018: Zoomorphology
https://www.readbyqxmd.com/read/29779281/-effects-of-tumor-necrosis-factor-%C3%AE-on-osteogenic-differentiation-and-notch-signaling-pathway-in-human-periodontal-ligament-stem-cells
#3
Yu Ma, Shu-Hui Li, Xin-Xin Ding, Pei-Ling Wu
OBJECTIVE: To evaluate the effects of tumor necrosis factor-α (TNF-α) on osteogenic differentiation and Notch signaling pathway of periodontal ligament stem cells (PDLSCs) and to investigate the regulatory role of Notch signaling pathway on the osteogenic differentiation of PDLSCs under the influence of TNF-α. METHODS: PDLSCs were obtained through enzyme digestion and tissue block method. The expression levels of stem cell surface markers CD105, CD90, CD146, CD45, and CD31 were detected by fluorescence activated cell sorter (FACS)...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779270/-protective-effects-of-hydrogen-rich-medium-on-lipopolysaccharides-induced-injury-in-human-periodontal-ligament-cells
#4
Min Zhou, Zuo-Lin Wang
OBJECTIVE: In this study, lipopolysaccharides (LPS) was used to damage human periodontal ligament cells (hPDLCs) and consequently investigate the protective effects of hydrogen on reducing oxidative stress and cell apoptosis rate. METHODS: hPDLCs were isolated, and then cultured with normal medium+1 μg·mL⁻¹ LPS or with hydrogen-rich medium+
1 μg·mL⁻¹ LPS. Cell proliferation activity was assessed using a cell counting kit-8 (CCK-8), and lactic dehydrogenase (LDH) release was also detected...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779212/periodontal-regeneration-via-chemo-attractive-constructs
#5
Xinjie Cai, Fang Yang, X Frank Walboomers, Yining Wang, John A Jansen, Jeroen J J P van den Beucken, Adelina S Plachokova
AIM: Chemo-attractants, such as stromal cell-derived factor-1α (SDF-1α), can offer an advantage for periodontal regeneration by recruiting the patient's own stem cells to stimulate self-repair. We here developed a chemo-attractive construct for periodontal regeneration using SDF-1α and evaluated its efficacy in vivo. MATERIALS AND METHODS: SDF-1α was loaded on gelatin sponge and tested in vitro for SDF-1α release. Subsequently, SDF-1α constructs were implanted into rat periodontal defects for 1 and 6 weeks, with unloaded materials and empty defects as controls...
May 19, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29776537/comparative-study-on-metabolite-level-in-tissue-specific-human-mesenchymal-stem-cells-by-an-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#6
Seul Ji Lee, TacGhee Yi, Soo Hyun Ahn, Dong Kyu Lim, Si-Na Kim, Hyun-Joo Lee, Yun-Kyoung Cho, Jae-Yol Lim, Jong-Hyuk Sung, Jeong-Ho Yun, Johan Lim, Sun U Song, Sung Won Kwon
Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29772080/substance-p-and-calcitonin-gene-related-peptide-expression-in-human-periodontal-ligament-after-root-canal-preparation-with-reciproc-blue-waveone-gold-xp-endoshaper-and-hand-files
#7
J Caviedes-Bucheli, N Rios-Osorio, M Rey-Rojas, F Laguna-Rivero, M M Azuero-Holguin, L E Diaz, H Curtidor, J J Castaneda-Ramirez, H R Munoz
AIM: To quantify the Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY: Fifty human periodontal ligament samples were obtained from healthy mandibular premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 40 of these premolars were equally divided into four groups, and root canals were prepared using four different systems: Reciproc Blue, WaveOne Gold, XP EndoShaper, and a hand instrumentation technique...
May 17, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29768044/cocl2-induces-apoptosis-via-ros-dependent-pathway-and-drp1-mediated-mitochondria-fission-in-the-periodontal-ligament-stem-cells
#8
Yuting He, Xueqi Gan, Ling Zhang, Beilei Liu, Zhuoli Zhu, Tao Li, Junfei Zhu, Junsheng Chen, Haiyang Yu
Oxygen deficiency is associated with various oral diseases, including chronic periodontitis, age-related alveolar bone loss and mechanical stress-linked cell injury from orthodontic appliances. Nevertheless, our understanding of the impact of hypoxia on periodontal tissues and its biochemical mechanism is still rudimentary. The purpose of this research was to elucidate the effects of hypoxia on the apoptosis of human periodontal ligament stem cells (PDLSCs) in vitro and the underlying mechanism. Herein, we showed that CoCl2 triggered cell dysfunction in human PDLSCs in a concentration-dependent manner and resulted in cell apoptosis and oxidative stress overproduction and accumulation in PDLSCs...
May 16, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29764006/tectorigenin-promotes-osteoblast-differentiation-and-in-vivo-bone-healing-but-suppresses-osteoclast-differentiation-and-in-vivo-bone-resorption
#9
So-Youn Lee, Gyu-Tae Kim, Hyung-Mun Yun, Youn-Chul Kim, Il-Keun Kwon, Eun-Cheol Kim
Although Tectorigenin (TG), a major compound in the rhizome of Belamcanda chinensis, is conventionally used for the treatment of inflammatory diseases, its effects on osteogenesis and osteoclastogenesis have not been reported. The objective of this study was to investigate the effects and possible underlying mechanism of TG on in vitro osteoblastic differentiation and in vivo bone formation, as well as in vitro osteoclast differentiation and in vivo bone resorption. TG promoted the osteogenic differentiation of primary osteoblasts and periodontal ligament cells...
May 15, 2018: Molecules and Cells
https://www.readbyqxmd.com/read/29761497/vibration-enhances-pge-2-il-6-and-il-8-expression-in-compressed-hpdl-cells-via-cyclooxygenase-pathway
#10
Pussadee Phusuntornsakul, Suwanna Jitpukdeebodintra, Prasit Pavasant, Chidchanok Leethanakul
BACKGROUND: Although vibration combined with orthodontic force may accelerate orthodontic tooth movement, little is known about the mechanisms that regulate the associated cellular responses. The propose of this study was to investigate whether mechanical vibration combined with compressive force regulates COX-2/PGE2 , IL-6 and IL-8 mRNA and protein expression in human periodontal ligament (hPDL) cells via the cyclooxygenase pathway. METHODS: The primary cultured hPDL cells were exposed to mechanical vibration, compressive force or a combination of both mechanical vibration and compressive force at 24, 48, 72 h...
May 14, 2018: Journal of Periodontology
https://www.readbyqxmd.com/read/29760824/the-impact-of-orthodontic-bands-on-the-marginal-periodontium-of-maxillary-first-molars-a-retrospective-cross-sectional-radiographic-analysis
#11
Sabine Teubner, Patrick R Schmidlin, Giorgio Menghini, Thomas Attin, Stefan Baumgartner
Aim: Available information on the effect of orthodontic treatment on crestal alveolar bone levels measured in radiographs is contradictory. The aim of this study was to compare the alveolar bone level and periodontal ligament space of banded upper first molars to untreated controls. Materials and Methods: This retrospective cross-sectional radiographic study investigated alveolar bone levels of upper first molars of an orthodontic test group and an untreated control group of comparable age (15-16...
2018: Open Dentistry Journal
https://www.readbyqxmd.com/read/29760819/antibacterial-and-anti-inflammatory-potential-of-morus-alba-stem-extract
#12
Ichaya Yiemwattana, Niratcha Chaisomboon, Kusuma Jamdee
Background: Periodontitis, a chronic inflammatory disease, is the leading cause of tooth loss in adults. Evidence for the anti inflammatory activity of M. alba Stem Extract (MSE) in periodontal disease is limited. Objective: The study aimed to investigate the inhibitory effect of MSE on the growth of periodontopathic bacteria and expression of interleukin (IL)-6 and IL-8 in Porphyromonas gingivalis Lipopolysaccharide (LPS)-stimulated human Periodontal Ligament (hPDL) fibroblasts...
2018: Open Dentistry Journal
https://www.readbyqxmd.com/read/29760736/comparison-of-immunological-characteristics-of-mesenchymal-stem-cells-from-the-periodontal-ligament-umbilical-cord-and-adipose-tissue
#13
Jin-Hee Kim, Chris H Jo, Hang-Rae Kim, Young-Il Hwang
Mesenchymal stem cells (MSCs) are of therapeutic importance in the fields of regenerative medicine and immunological diseases. Accordingly, studies evaluating MSCs for clinical applications are increasing. In this study, we characterized MSCs from the periodontal ligament, umbilical cord (UC-MSCs), and adipose tissue, which were relatively easy to obtain with limited ethical concerns regarding their acquisition, and compared their immunological characteristics. Among MSCs isolated from the three different tissues, UC-MSCs grew the fastest in vitro ...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29758960/biomechanical-investigation-of-orthodontic-treatment-planning-based-on-orthodontic-force-measurement-and-finite-element-method-before-implementation-a-case-study
#14
Jianlei Wu, Yunfeng Liu, Jianxing Zhang, Wei Peng
BACKGROUND: Orthodontic treatment planning (OTP) is primarily depended on clinical experiences of orthodontists at present, while equivocal OTP would increase the possibility of treatment failure. OBJECTIVE: The objective was to investigate a methodology for quantitatively evaluating OTP, using theoretical analyses, orthodontic forces measurement (OFM) and finite element method (FEM). METHODS: An OTP was theoretically designed based on a clinical case and forces on incisors in OTP were measured on a specialized platform...
April 27, 2018: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://www.readbyqxmd.com/read/29756133/biology-of-teeth-and-implants-host-factors-pathology-regeneration-and-the-role-of-stem-cells
#15
F-Michael Eggert, Liran Levin
In chronic periodontitis and peri-implantitis, cells of the innate and adaptive immune systems are involved directly in the lesions within the tissues of the patient. Absence of a periodontal ligament around implants does not prevent a biologic process similar to that of periodontitis from affecting osseointegration. Our first focus is on factors in the biology of individuals that are responsible for the susceptibility of such individuals to chronic periodontitis and to peri-implantitis. Genetic factors are of significant importance in susceptibility to these diseases...
2018: Quintessence International
https://www.readbyqxmd.com/read/29750093/laser-phototherapy-induces-angiogenesis-in-the-periodontal-tissue-after-delayed-tooth-replantation-in-rats
#16
Felipe-de Souza Matos, Fernanda-de Jesus Godolphim, Ricardo-Luiz-Cavalcanti Albuquerque-Júnior, Luiz-Renato Paranhos, Sigmar-de Mello Rode, Cláudio-Antonio-Talge Carvalho, Maria-Amália-Gonzaga Ribeiro
Background: Laser phototherapy (LPT) has been suggested as a new therapeutic tool to improve the repair of replanted teeth. However, its effects and mechanism of action are not yet completely understood. Objectives: This study evaluated histologically the effect of laser phototherapy (LPT) λ808 and λ660 nm on angiogenesis in the periodontal tissue of replanted teeth in rats. Material and Methods: Twenty maxillary right incisors were extracted from twenty Wistar rats and randomly assigned to four groups (n = 5): PN - teeth were stored in paper napkin for 45 min; WM - teeth were immersed in 20 ml of UHT whole cow milk for 45 min; PNL and WML - teeth received the same treatment described for PN and WM, respectively, plus LPT at λ808 and λ660 nm...
April 2018: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/29744214/comparison-of-biofilm-formation-and-migration-of-streptococcus-mutans-on-tooth-roots-and-titanium-miniscrews
#17
Kittipong Laosuwan, Don Jeevanie Epasinghe, Zhaoming Wu, Wai Keung Leung, David William Green, Han Sung Jung
Periodontitis and peri-implantitis are inflammatory diseases caused by periodontal pathogenic bacteria leading to destruction of supporting periodontal/peri-implant tissue. However, the progression of inflammatory process of these two diseases is different. The bacterial biofilm is the source of bacteria during the inflammatory process. As the bacteria migrate down the surface of tooth or titanium implant, the inflammation spreads along with it. Streptococcus mutans has an important role in oral bacterial biofilm formation in early stage biofilm before the microbiota shift to late stage and become more virulent...
April 2018: Clinical and Experimental Dental Research
https://www.readbyqxmd.com/read/29744190/metal-ions-from-s-prg-filler-have-the-potential-to-prevent-periodontal-disease
#18
Yoko Iwamatsu-Kobayashi, Syouta Abe, Yoshiyasu Fujieda, Ai Orimoto, Masafumi Kanehira, Keisuke Handa, Venkata Suresh Venkataiah, Wei Zou, Masaki Ishikawa, Masahiro Saito
The surface pre-reacted glass ionomer (S-PRG) filler, a component of composite resin, is capable of releasing metal ions that possess antibacterial activity against caries and periodontal pathogens. Although S-PRG has been suggested to be involved in oral disease prevention, no reports have been published regarding its preventive effect on periodontal disease in vivo . The present study investigated whether the eluate from S-PRG (S-PRG eluate) has a suppressive effect on tissue destruction induced in a mouse model of ligature-induced periodontal disease...
August 2017: Clinical and Experimental Dental Research
https://www.readbyqxmd.com/read/29742232/can-low-fusing-glass-application-affect-the-marginal-misfit-and-bond-strength-of-y-tzp-crowns
#19
Monize Carelli Felipe Antunes, Jean Soares Miranda, Ronaldo Luís Almeida de Carvalho, Rodrigo Furtado de Carvalho, Estevão Tomomitsu Kimpara, Rodrigo Othávio de Assunção E Souza, Fabíola Pessôa Pereira Leite
To evaluate the effect of different surface treatments on the marginal misfit and retentive strength between Y-TZP crowns and an epoxy resin. Forty (40) epoxy resin (G10) abutments (height: 5mm, conicity: 60, finish line: large chamfer) with equal dimensions were milled and included in polyurethane to simulate the periodontal ligament. Next, 40 Y-TZP crowns (thickness: 1mm) were milled (Cerec in Lab) and randomly divided into four groups (n=10) according to the surface treatment: GS(glaze spray), GP(glaze powder/liquid), P(zirconia primer) and RS(tribochemical silica coating)...
2018: Brazilian Oral Research
https://www.readbyqxmd.com/read/29733059/citotoxicidad-de-soluciones-recomendadas-para-el-almacenamiento-de-dientes-avulsionados-en-cultivo-con-c%C3%A3-lulas-del-ligamento-periodontal
#20
Galia Cruz-Durán, Raúl Ortiz-Daza Raúl, Jacinto Armando Díaz-Acevedo, Benjamín Sánchez-Trocino, Ma Concepción Arenas-Arrocena, René García-Contreras
Introducción: El medio de almacenamiento de los dientes avulsionados hasta su reimplante es vital para conservar los fibroblastos del ligamento periodontal humano (HPLF). Objetivo: Comparar el efecto citotóxico para conservar los HPLF de la leche y la solución isotónica para almacenamiento de dientes avulsionados. Método: Se realizó subcultivo de fibroblastos del ligamento periodontal con una densidad de 1:2 (3 × 105 células/mL), que fueron incubados por 48 horas...
2018: Gaceta Médica de México
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