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Periodontal ligament

Ahmad Alobeid, Tarek El-Bialy, Susanne Reimann, Ludger Keilig, Dirk Cornelius, Andreas Jäger, Christoph Bourauel
Background/objective: The aim of this study was to evaluate the efficacy of tooth alignment with conventional and self-ligating labial and lingual orthodontic bracket systems. Materials/methods: We tested labial brackets (0.022″ slot size) and lingual brackets (0.018″ slot size). The labial brackets were: (i) regular twin brackets (GAC-Twin [Dentsply]), (ii) passive self-ligating brackets including (Damon-Q® [ORMCO]; Ortho classic H4™ [Orthoclassic]; FLI®SL [RMO]), and (iii) active self-ligating brackets (GAC In-Ovation®C [DENTSPLY] and SPEED™[Strite])...
March 13, 2018: European Journal of Orthodontics
He Wang, Jianjia Li, Xiaoyi Zhang, Tingting Ning, Dandan Ma, Yihong Ge, Shuaimei Xu, Yilin Hao, Buling Wu
To seek a potential target for periodontal tissue regeneration, this study aimed to explore the role of Integrin alpha 5 (ITGA5) in human periodontal ligament stem cells (PDLSCs). Transwell assay, Cell Counting Kit 8 (CCK8) assay, cell cycle assay, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot were used to investigate the effects of ITGA5 on PDLSC migration, proliferation and osteogenic differentiation. The in vivo effect was investigated by nude mice subcutaneous transplantation with cell and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) complex...
March 12, 2018: Journal of Proteomics
Marjan Nokhbehsaim, Svenja Memmert, Anna Damanaki, Shanika Nanayakkara, Xiaoyan Zhou, Andreas Jäger, James Deschner
OBJECTIVES: Periodontopathogens induce immunoinflammatory responses characterized by the release of inflammatory mediators, e.g., interleukin (IL)-1β, IL-6, and IL-8. Ghrelin (GHRL) is an appetite hormone which mediates its effect via the functional receptor GHS-R1a. This study was to examine the effect of an inflammatory insult on GHS-R1a in human periodontal cells. MATERIALS AND METHODS: Periodontal ligament (PDL) cells and gingival fibroblasts (HGFs) were exposed to IL-1β in the presence and absence of GHRL...
March 14, 2018: Clinical Oral Investigations
Qian Li, Jiao Chen, Xiaoying Li, Bomiao Cui, Yaping Fan, Ning Geng, Qianming Chen, Ping Zhang, Yun Feng
High mobility group nucleosomal-binding domain 2 (HMGN2) is an abundant non-histone nuclear protein of vertebrates and invertebrates. The aim of the present study was to characterize the endogenous expression of HMGN2 in various types of tumor cell. Western blotting was performed to analyze HMGN2 expression in the following tumor cell lines: H1975, HSC-4, MDA-MB-468, MDA-MB-231, SCC-25 and THP-1. Periodontal ligament cells (PDLCs) were included as a noncancerous control. HMGN2 was detected in human oral squamous cell carcinoma tissues by immunohistochemical analysis...
April 2018: Oncology Letters
Gary Hin-Fai Yam, Ericia Pei-Wen Teo, Melina Setiawan, Matthew J Lovatt, Nur Zahirah Binte M Yusoff, Matthias Fuest, Bee-Tin Goh, Jodhbir S Mehta
Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest...
March 13, 2018: Journal of Cellular and Molecular Medicine
Anamaria Balic
The tooth is an intricate composition of precisely patterned, mineralized matrices and soft tissues. Mineralized tissues include enamel (produced by the epithelial cells called ameloblasts), dentin and cementum (produced by mesenchymal cells called odontoblasts and cementoblasts, respectively), and soft tissues, which include the dental pulp and the periodontal ligament along with the invading nerves and blood vessels. It was perceived for a very long time that teeth primarily serve an esthetical function. In recent years, however, the role of healthy teeth, as well as the impact of oral health on general well-being, became more evident...
March 13, 2018: Gerontology
M Wolf, M Ao, M B Chavez, T N Kolli, V Thumbigere-Math, K Becker, E Y Chu, A Jäger, M J Somerman, B L Foster
Previous studies revealed that cementum formation is tightly regulated by inorganic pyrophosphate (PPi ), a mineralization inhibitor. Local PPi concentrations are determined by regulators, including ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which increases PPi concentrations by adenosine triphosphate hydrolysis. Orthodontic forces stimulate alveolar bone remodelling, leading to orthodontic tooth movement (OTM). To better understand how disturbed mineral metabolism and the resulting altered periodontal structures affect OTM, we employed Enpp1 mutant mice that feature reduced PPi and increased cervical cementum in a model of OTM induced by a stretched closed-coil spring ligated between the maxillary left first molar and maxillary incisors...
March 1, 2018: Journal of Dental Research
Mostafa Assem, Samia Kamal, Dina Sabry, Nadia Soliman, Riham M Aly
BACKGROUND: Stem cells have recently received great interest as potential therapeutics alternative for a variety of diseases. The oral and maxillofacial region, in particular, encompasses a variety of distinctive mesenchymal (MSC) populations and is characterized by a potent multilineage differentiation capacity. AIM: In this report, we aimed to investigate the effect of diabetes on the proliferation potential of stem cells isolated from controlled diabetic patients (type 2) and healthy individuals...
February 15, 2018: Open Access Macedonian Journal of Medical Sciences
Damla Tuncer Budanur, Noushin Zibandeh, Deniz Genç, Muazzez Gökalp, Kamber Kaşali, Tunç Akkoç, Elif Sepet
BACKGROUND/AIM: Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators' effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells (hPDLMSCs). MATERIALS AND METHODS: hPDLMSCs were obtained from impacted third molar teeth...
March 12, 2018: Dental Traumatology: Official Publication of International Association for Dental Traumatology
Nobuhiro Yamauchi, Yoichiro Taguchi, Hirohito Kato, Makoto Umeda
BACKGROUND: Light-emitting diode (LED) is attracting attention as a new light source for phototherapy. However, its effects on periodontal tissue regeneration remain unknown. The aim of this study was to examine the effects of high-power, red LED irradiation on human periodontal ligament stem cells (PDLSCs), which play an important role in periodontal tissue regeneration. METHODS: PDLSCs were derived from adult human third molars. The light source was red LED (peak wavelength: 650 nm)...
March 12, 2018: Journal of Periodontology
Tingting Ai, Jieni Zhang, Xuedong Wang, Xiaowen Zheng, Xueyan Qin, Qian Zhang, Weiran Li, Wei Hu, Jiuxiang Lin, Feng Chen
Among the various sources of human autologous stem cells, stem cells isolated from dental tissues exhibit excellent properties in tissue engineering and regenerative medicine. However, the distinct potential of these odontogenic cell lines remains unclear. In this study, we analyzed DNA methylation patterns to determine whether specific differences existed among three different odontogenic cell types. Using the HumanMethylation450 Beadchip, the whole genomes of human dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs), and dental follicle progenitor cells (DFPCs) were compared...
2018: Signal Transduction and Targeted Therapy
U Kandalam, N Ledra, H Laubach, K V Venkatachalam
BACKGROUND AND OBJECTIVES: Pathogenic infections caused by Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia can result in the production of volatile sulfur compounds (VSC's) and other toxic compounds from methionine catabolism that can lead to halitosis and periodontitis. Our aim is to block the activity of methionine gammalyase-deaminase (Mgld) of methionine catabolism to prevent halitosis/periodontitis. DESIGNS: Cloned, expressed, Mgld protein was tested for purity by SDS-PAGE and western blotting...
March 1, 2018: Archives of Oral Biology
Gustavo Assis de Paula, Guilherme Carvalho Silva, Ênio Lacerda Vilaça, Tulimar Machado Cornacchia, Cláudia Silami de Magalhães, Allyson Nogueira Moreira
PURPOSE: Biomechanical behavior of tooth-implant-supported prostheses (TISPs) with external and internal implants was compared. MATERIALS AND METHODS: Two 3-D models of TISP were designed by varying the implant: external (Model EH) and internal hexagons (Model IH). After loading, von Mises stresses were obtained in implants, abutments, and screws. Principal maximum (σmax) and minimum (σmin) stresses were analyzed in periodontal ligament (PL), alveolar bone, and periimplant bone...
March 7, 2018: Implant Dentistry
Zhanyi Gao, Kaining Liu, Huanxin Meng
BACKGROUND: The vitamin D pathway, from toll-like receptor activation to human cationic antimicrobial protein (hCAP-18/LL-37) generation, has been identified in monocytes and keratinocytes. This study aimed to investigate the vitamin D pathway in human gingival fibroblasts (hGFs) and periodontal ligament cells (hPDLCs) and to provide preliminary evidence of its role in periodontal immune defense. METHODS: Primary cultures of hGFs and hPDLCs were stimulated with 1,25-dihydroxy vitamin D3 and 25-hydroxy vitamin D3 , with or without Porphyromonas gingivalis lipopolysaccharide...
February 18, 2018: Journal of Periodontology
Atika Resti Fitri, Prasit Pavasant, Supakarn Chamni, Piyamas Sumrejkanchanakij
BACKGROUND: Asiaticoside is a compound isolated from Herb Centella asiatica, which has been shown to promote osteogenic differentiation of human periodontal ligament (hPDL) cells. This study investigated the molecular mechanism underlying the asiaticoside-induced osteogenic differentiation of hPDL cells. METHODS: hPDL cells were incubated with various concentrations of asiaticoside to test cell viability by MTT assay. The mRNA expression levels were analyzed by using quantitative real-time polymerase chain reaction (PCR)...
February 22, 2018: Journal of Periodontology
Xiang-Zhen Yan, Jeroen J J P van den Beucken, Chunxue Yuan, John A Jansen, Fang Yang
OBJECTIVE: The therapeutic potential of periodontal ligament cells for periodontal regeneration gradually decreases when cultured as a monolayer in vitro. Three-dimensional cell culture models provide an alternative to traditional monolayer cell culture. This study aimed to comparatively evaluate the influence of spheroid culture on periodontal ligament cells. MATERIALS AND METHODS: Chitosan films were used to culture three-dimensional periodontal ligament cell spheroids...
March 7, 2018: Oral Diseases
Daniela Atili Brandini, Marina Fuzette Amaral, Caio Vinícius Lourenço Debortoli, Sônia Regina Panzarini
The aim of this study is to evaluate the action of paramonochlorophenol associated with Furacin followed by calcium hydroxide (CH) dressing in the control of inflammatory root resorption in cases of immediate tooth replantation with delayed endodontic treatment. A total of 28 incisors of 3 male dogs were extracted and replanted after 15 minutes, and randomly divided into 3 groups: Group I (n = 8) - endodontic treatment was performed before the extraction and replantation; Group II (n = 10) - endodontic treatment was performed 30 days after replantation and the root canal was filled with CH dressing; Group III (n = 10) - endodontic treatment was performed 30 days after replantation and root canals received temporary medication of paramonochlorophenol-Furacin followed by CH dressing...
March 1, 2018: Brazilian Oral Research
Iman Z Oskui, Ata Hashemi, Hamid Jafarzadeh, Akiko Kato
BACKGROUND AND OBJECTIVE: Traumatic loading is the main form of injury sustained in dental injuries. In spite of the prevalence of dental trauma, little information is available on traumatic dental damage and the evaluation of tooth behavior under traumatic loading. Due to the short period of traumatic loading, at first sight, a dynamic analysis needs to be performed to investigate the dental trauma. However, it was hypothesized that dental traumatic loading could be regarded as quasi-static loading...
March 2018: Computer Methods and Programs in Biomedicine
Xiaoshan Wu, Lei Hu, Yan Li, Yang Li, Fu Wang, Ping Ma, Jinsong Wang, Chunmei Zhang, Canhua Jiang, Songlin Wang
The tooth root transmits and balances occlusal forces through the periodontium to the alveolar bone. The periodontium, including the gingiva, the periodontal ligament, the cementum and the partial alveolar bone, derives from the dental follicle (DF), except for the gingiva. In the early developmental stages, the DF surrounds the tooth germ as a sphere and functions to promote tooth eruption. However, the morphological dynamics and factors regulating the differentiation of the DF during root elongation remain largely unknown...
2018: International Journal of Medical Sciences
F Tirone, S Salzano, A Piattelli, V Perrotti, G Iezzi
OBJECTIVES: The aim of this study was to investigate for the first time the histological response of periodontium to mineral trioxide aggregate (MTA) and Biodentine in human experimental models. MATERIALS AND METHODS: Six patients scheduled for implant full-arch rehabilitation via the all-on-4 technique were randomly assigned to one of the two test groups: either to MTA or Biodentine treatment. For each patient, 2 teeth scheduled for strategic extraction were randomly assigned either to the test or to the control treatment...
March 6, 2018: Australian Dental Journal
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