keyword
MENU ▼
Read by QxMD icon Read
search

Intracellular Flow Cytometry

keyword
https://www.readbyqxmd.com/read/29350596/enhancement-of-antitumor-activity-by-using-5-ala-mediated-sonodynamic-therapy-to-induce-apoptosis-in-malignant-gliomas-significance-of-high-intensity-focused-ultrasound-on-5-ala-sdt-in-a-mouse-glioma-model
#1
Satoshi Suehiro, Takanori Ohnishi, Daisuke Yamashita, Shohei Kohno, Akihiro Inoue, Masahiro Nishikawa, Shiro Ohue, Junya Tanaka, Takeharu Kunieda
OBJECTIVE High invasiveness of malignant gliomas frequently causes early local recurrence of the tumor, resulting in extremely poor outcome. To control such recurrence, novel therapies targeted toward infiltrating glioma cells around the tumor border are required. Here, the authors investigated the antitumor activity of sonodynamic therapy (SDT) combined with a sonosensitizer, 5-aminolevulinic acid (5-ALA), on malignant gliomas to explore the possibility for clinical use of 5-ALA-mediated SDT (5-ALA-SDT). METHODS In vitro cytotoxicity of 5-ALA-SDT was evaluated in U87 and U251 glioma cells and in U251Oct-3/4 glioma stemlike cells...
January 19, 2018: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29348654/low-concentration-trifluoperazine-promotes-proliferation-and-reduces-calcium-dependent-apoptosis-in-glioma-cells
#2
Yulin Wen, Yong Zhang, Jinbang Li, Feng Luo, Zhongxi Huang, Kunping Liu
Glioma patients constitute the greatest percentage of depressed neoplasm patients. These patients often require antidepressant treatment, but the effect of antidepressant drugs on glioma cells requires further evaluation. In the present study, we evaluated the effect of trifluoperazine (TFP) on the proliferation and apoptosis of glioma cells. Transcriptomic and bioinformatics analysis results suggested that antidepressant drugs, especially TFP, may upregulate the drug-resistant ability of glioma cells. A low concentration of TFP upregulated the viability of glioma cells...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29346642/rnase7-but-not-psoriasin-nor-spla2-iia-associates-to-mycobacterium-tuberculosis-during-airway-epithelial-cells-infection
#3
Flor Torres-Juarez, Lousseine Touqui, Juan Leon-Contreras, Cesar Rivas-Santiago, Jose A Enciso-Moreno, Rogelio Hernández-Pando, Bruno Rivas-Santiago
Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis (Mtb). Innate immunity is the first line of defense against Mtb and malfunctions in any of its components is associated with the susceptibility to the disease. Epithelial products, such as host defence peptides (HDP) are the first molecules produced to counteract the infection. Although a wide variety of HDP are produced by epithelial cells only few of them have been studied during Mtb infection. Here, we assessed the expression and production of the HDPs: psoriasin, sPLA2-IIA and RNase 7 in airway epithelial cells (NCI-H292), type II pneumocytes (A549 cells) and monocytes-derived macrophages from human peripheral blood mononuclear cells (bMDM) and from the human cell line THP1(MDM) after Mtb in vitro infection...
January 16, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29345354/lack-of-functional-selectin-ligand-interactions-enhances-innate-immune-resistance-to-systemic-listeria-monocytogenes-infection
#4
Gerard Agbayani, Komal Gurnani, Ahmed Zafer, Subash Sad, Lakshmi Krishnan
Selectin-ligand interactions are important for leukocyte homing and functionality. The roles of selectin-ligand interactions in modulating immunity to intracellular infections are not completely understood. Mice lacking the expression of fucosyltransferase-IV and -VII (Fucosyltransferase-IV and -VII double knockout, FtDKO) exhibit deficient functionality of selectin-ligand interactions. We addressed the kinetics of infection and immunity to Listeria monocytogenes (LM), an intracellular pathogen, in FtDKO mice...
December 27, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29345335/heat-shock-protein-70-modulates-neural-progenitor-cells-dynamics-in-human-neuroblastoma-sh-sy5y-cells-exposed-to-high-glucose-content
#5
Leila Salimi, Reza Rahbarghazi, Vahab Jafarian, Çıgır Biray Avci, Bakiye Goker Bagca, Neslihan Pinar Ozates, Majid Khaksar, Alireza Nourazarian
BACKGROUND: In the current experiment, detrimental effects of high glucose condition were investigated on human neuroblastoma cells. METHODS: Human neuroblastoma cell line SH-SY5Y were exposed to 5, 40 and 70 mM glucose over a period of 72 h. Survival rate and the proliferation of cells were analyzed by MTT and BrdU incorporation assays. Apoptosis was studied by the assays of flow cytometry and PCR array. In order to investigate the trans-differentiation capacity of the cell into mature neurons, we used immunofluorescence imaging to follow NeuN protein level...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29344233/lidamycin-decreases-cd133-expression-in-hepatocellular-carcinoma-via-the-notch-signaling-pathway
#6
Yi Chen, Wenwei Sun, Ran He, Feiyan Zhang, Hongyu Wang, Panhong Li, Rong-Guang Shao, Xiaoyu Xu
Cluster of differentiation (CD)133 is considered a molecular marker of cancer stem cells in hepatocellular carcinoma. In the present study, the effect of lidamycin (LDM) on CD133 expression in hepatocellular carcinoma (Huh7 cells) was evaluated and the potential molecular mechanism was investigated. Flow cytometry analysis, as well as sorting, sphere formation and western-blot assays, were performed in vitro to explore the effects of LDM on CD133 expression. A subcutaneous tumor model in nude mice was used to observe the effects of LDM on tumor volume and CD133 protein in vivo...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344187/patuletin-induces-apoptosis-of-human-breast-cancer-sk-br-3-cell-line-via-inhibiting-fatty-acid-synthase-gene-expression-and-activity
#7
Wanwan Zhu, Chunmei Lv, Jiao Wang, Qiang Gao, Hui Zhu, Haixia Wen
Fatty acid synthase (FASN) is a key enzyme involved in fatty acid biosynthesis and serves an important role in breast cancer development. The aim of the present study was to investigate the effects of patuletin on the gene expression and activity of FASN in the human breast cancer SK-BR-3 cell line, and the apoptotic effects of patuletin to breast cancer cells. Quantitative reverse transcription polymerase chain reaction, western blotting and intracellular FASN activity assays were used to evaluate FASN gene expression, protein expression and activity in patuletin-treated SK-BR-3 cells...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29343959/antitumor-effect-of-a-new-nano-vector-with-mirna-135a-on-malignant-glioma
#8
Chaofeng Liang, Weitong Sun, Haiyong He, Baoyu Zhang, Cong Ling, Bocheng Wang, Tengchao Huang, Bo Hou, Ying Guo
Introduction: MiR-135a is found to selectively induce apoptosis in glioma cell but not in normal neurons and glial cells. However, low transfection efficacy limits its application in vivo as other miRNAs. We prepared a new kind of nano-vector based on polyethylene glycol methyl ether (mPEG) and hyper-branched polyethylenimine (hy-PEI) in order to improve the miRNA delivery system into the glioma cells. Methods: The mPEG-g-PEI/miR-135a was constructed and detected by 1H NMR and FTIR analyses...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29343447/altered-b-lymphocyte-homeostasis-and-functions-in-systemic-sclerosis
#9
REVIEW
Alexandra Forestier, Thomas Guerrier, Mathieu Jouvray, Jonathan Giovannelli, Guillaume Lefèvre, Vincent Sobanski, Carine Hauspie, Eric Hachulla, Pierre-Yves Hatron, Hélène Zephir, Patrick Vermersch, Myriam Labalette, David Launay, Sylvain Dubucquoi
Beyond the production of autoantibodies, B-cells are thought to play a role in systemic sclerosis (SSc) by secreting proinflammatory/profibrotic cytokines. B-cells are a heterogeneous population with different subsets distinguished by their phenotypes and cytokine production. Data about B-cell subsets, cytokine production and intracellular pathways leading to this production are scarce in SSc. The aim of our study was to describe B-cell homeostasis, activation, proliferation, cytokine production in B-cells and serum and B-cell intracellular signaling pathways in SSc...
January 14, 2018: Autoimmunity Reviews
https://www.readbyqxmd.com/read/29337047/imaging-flow-cytometry-a-method-for-examining-dynamic-native-foxo1-localization-in-human-lymphocytes
#10
Molly K Hritzo, Jean-Paul Courneya, Amit Golding
While flow cytometry can reliably assess surface and intracellular marker expression within small cell populations, it does not provide any information on protein localization. Several key transcription factors (TF) downstream of lymphocyte surface receptors are regulated by nuclear versus cytoplasmic localization, and one such TF is Forkhead box O1 (FOXO1). FOXO1 integrates antigen-binding, co-receptor activation and metabolic signals in lymphocytes, leading to proliferation and differentiation. Importantly, the nuclear or cytoplasmic localization of FOXO1 is key for gene expression leading to different lymphocyte phenotypes...
January 11, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29336467/nox4-ros-mediate-ethanol%C3%A2-induced-apoptosis-via-mapk-signal-pathway-in-l%C3%A2-02-cells
#11
Cheng-Fang Yang, Yu-Juan Zhong, Zuheng Ma, Li Li, Lin Shi, Li Chen, Chen Li, Dan Wu, Qi Chen, Yong-Wen Li
The aim of the present study was to assess the molecular mechanism of ethanol‑induced oxidative stress‑mediated apoptosis in L‑02 liver cells in order to elucidate novel pathways associated with alcoholic liver disease. L‑02 cells were treated with 400 mM ethanol with or without inhibitors. The cell viability was measured by an MTT assay. Cell apoptosis was assessed by flow cytometry and a single‑stranded DNA (ssDNA) assay. Intracellular reactive oxygen species (ROS) production of L‑02 cells was determined using the 2',7'‑dichlorofluorescein‑diacetate dye...
January 16, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29334216/lysosome-independent-intracellular-drug-gene-co-delivery-by-lipoprotein-derived-nanovector-for-synergistic-apoptosis-inducing-cancer-targeted-therapy
#12
Wei Wang, Kerong Chen, Yujie Su, Jielei Zhang, Min Li, Jianping Zhou
In this paper, reconstituted high-density lipoprotein (rHDL), a lipoprotein-derived nanovector, were constructed for co-delivery of paclitaxel (PTX) and wild type p53 gene (p53). The particle size and the zeta potential of PTX-DODAB/p53-rHDL nanoparticles were 177.2 nm and -20.06 mV, respectively. Meanwhile, they exhibited great serum stability and satisfactory sustained release characteristics in vitro. PTX-DODAB/pDNA-rHDL nanoparticles simultaneously improved the cellular uptake of PTX and pDNA via scavenger receptor B type I (SR-BI) mediated lysosome-independent internalization and promoted the transfection of pDNA in MCF-7 cells, which were revealed by flow cytometry and confocal laser scanning microscopy analyses...
January 15, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29331753/an-iridium-iii-complex-as-potent-anticancer-agent-induces-apoptosis-and-autophagy-in-b16%C3%A2-cells-through-inhibition-of-the-akt-mtor-pathway
#13
Bing Tang, Dan Wan, Yang-Jie Wang, Qiao-Yan Yi, Bo-Hong Guo, Yun-Jun Liu
A new ligand THPDP (THPDP = 11-(6,7,8,9-tetrahydrophenazin-2-yl)dipyrido[3,2-a:2',3'-c]phenazine) and its iridium(III) complex [Ir(ppy)2(THPDP)]PF6 (Ir-1) was synthesized and characterized by elemental analysis, IR, ESI-MS, 1H NMR and 13C NMR. The cytotoxicity in vitro of the complex against cancer cells B16, A549, Eca-109, SGC-7901, BEL-7402 and normal NIH 3T3 cell lines was evaluated using MTT method. The IC50 values of the complex toward B16, A549 and Eca-109 cells are 1.0 ± 0.02, 1.4 ± 0...
December 30, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29328950/ultrastructural-damage-in-streptococcus-mutans-incubated-with-saliva-and-histatin-5
#14
A M Fernández-Presas, Y Márquez Torres, R García González, A Reyes Torres, I Becker Fauser, H Rodríguez Barrera, B Ruíz García, R Toloza Medina, J Delgado Domínguez, J L Molinarí Soriano
OBJECTIVE: To study the ultrastructural alterations induced in Streptococcus mutans (ATCC 25175) incubated with saliva, saliva plus histatin 5 and histatin 5. METHODS: S. mutans incubated with saliva histatin 5 or a combination of both were morphologically analyzed and counted. The results were expressed as (CFU)ml-1. Ultrastructural damage was evaluated by transmission electron microscopy. Ultrastructural localization of histatin 5 was examined using immunogold labeling...
January 8, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29323101/actin-filament-associated-protein-1-like-1-mediates-proliferation-and-survival-in-non-small-cell-lung-cancer-cells
#15
Meng Wang, Xingpeng Han, Wei Sun, Xin Li, Guohui Jing, Xun Zhang
BACKGROUND The actin filament-associated protein (AFAP) family consists of 3 novel adaptor proteins: AFAP1, AFAP1L1, and AFAP1L2/XB130. Although evidence shows that AFAP1 and AFAP1L2 play an oncogenic role, the effect of AFAP1L1 on tumor cell behavior has not been fully elucidated, and it remains unknown whether AFAP1L1 could be a prognostic marker and/or therapeutic target of lung cancer. MATERIAL AND METHODS Human A549 non-small cell lung cancer (NSCLC) cells were used in this study. AFAP1L1 gene was knocked down by AFAP1L1 short hairpin RNA (shRNA) transfection...
January 11, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29321629/chiloscyphenol-a-derived-from-chinese-liverworts-exerts-fungicidal-action-by-eliciting-both-mitochondrial-dysfunction-and-plasma-membrane-destruction
#16
Sha Zheng, Wenqiang Chang, Ming Zhang, Hongzhuo Shi, Hongxiang Lou
This study aimed to characterize the antifungal effects of chiloscyphenol A (CA), a natural small molecule isolated from Chinese liverworts, and investigate its mode of action. CA was effective against five tested Candida species with a minimal inhibitory concentration (MIC) of 8-32 μg/ml and exhibited fungicidal activity against Candida albicans in both the planktonic state and mature biofilms. The in vivo study using Caenorhabditis elegans showed that CA prolonged the survival of C. albicans infected worms...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29318976/algerian-propolis-potentiates-doxorubicin-mediated-anticancer-effect-against-human-pancreatic-panc-1-cancer-cell-line-through-cell-cycle-arrest-apoptosis-induction-and-p-glycoprotein-inhibition
#17
Hassiba Rouibah, Lahouel Mesbah, Wided Kebsa, Malek Zihlif, Mamoun Ahram, Bachaer Aburmeleih, Ibtihal Mostafa, Hemzeh El Amir
BACKGROUND: Pancreatic cancer is one of the most aggressive and lethal cancer, with poor prognosis and high resistant to current chemotherapeutic agents. Therefore, new therapeutic strategies and targets are underscored. Propolis has been reported to exhibit a broad spectrum of biological activities including anticancer activity. OBJECTIVE: This study was carried out to assess the possible efficacy of Algerian propolis on the antitumor effect of doxorubicin on human pancreatic cancer cell line (PANC-1)...
January 10, 2018: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29316248/a-standardized-flow-cytometry-procedure-for-the-monitoring-of-regulatory-t-cells-in-clinical-trials
#18
Pitoiset Fabien, Barbié Michèle, Monneret Guillaume, Braudeau Cécile, Pochard Pierre, Pellegrin Isabelle, Trauet Jacques, Labalette Myriam, Klatzmann David, Rosenzwajg Michelle
BACKGROUND: Quantification of regulatory T cells is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections and allergies. Human regulatory T cells are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29306537/metformin-enhances-the-differentiation-of-dental-pulp-cells-into-odontoblasts-by-activating-ampk-signaling
#19
Wei Qin, Xianling Gao, Tao Ma, Michael D Weir, Jing Zou, Bing Song, Zhengmei Lin, Abraham Schneider, Hockin H K Xu
INTRODUCTION: Metformin is a first-line drug for treating type 2 diabetes that regulates the differentiation of mesenchymal stem cells. Its effects on human dental pulp cells (DPCs) remain unknown. This study aimed to investigate the effects of metformin on the proliferation and differentiation of DPCs. METHODS: A live/dead viability assay kit was used to examine the effects of metformin on the cell viability of DPCs. Cell proliferation was analyzed using a cell counting kit (CCK-8; Dojindo, Tokyo, Japan)...
January 3, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29306331/fc-gamma-receptors-are-expressed-in-the-developing-rat-brain-and-activate-downstream-signaling-molecules-upon-cross-linking-with-immune-complex
#20
Marianna Stamou, Ana Cristina Grodzki, Marc van Oostrum, Bernd Wollscheid, Pamela J Lein
BACKGROUND: Exposure of the developing brain to immune mediators, including antibodies, is postulated to increase risk for neurodevelopmental disorders and neurodegenerative disease. It has been suggested that immunoglobulin G-immune complexes (IgG-IC) activate Fc gamma receptors (FcγR) expressed on neurons to modify signaling events in these cells. However, testing this hypothesis is hindered by a paucity of data regarding neuronal FcγR expression and function. METHODS: FcγR transcript expression in the hippocampus, cortex, and cerebellum of neonatal male and female rats was investigated ex vivo and in mixed cultures of primary hippocampal and cortical neurons and astrocytes using quantitative PCR analyses...
January 6, 2018: Journal of Neuroinflammation
keyword
keyword
81339
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"