keyword
https://read.qxmd.com/read/38547470/efficient-markerless-genetic-manipulation-of-pasteurella-multocida-using-lacz-and-phes-m-as-selection-markers
#1
JOURNAL ARTICLE
Jinfei Jiang, Yishan Zhao, Aihua Chen, Juan Sun, Mengruo Zhou, Jialian Hu, Xuewei Cao, Ning Dai, Zhaoping Liang, Saixiang Feng
Pasteurella multocida is a zoonotic conditional pathogen that infects multiple livestock species, causing substantial economic losses in the animal husbandry industry. An efficient markerless method for gene manipulation may facilitate the investigations of P. multocida gene function and pathogenesis of P. multocida . Herein, a temperature-sensitive shuttle vector was constructed using lacZ as a selection marker, and markerless glgB , opa , and hyaE mutants of P. multocida were subsequently constructed through blue-white colony screening...
March 28, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38547321/a-novel-sorf-gene-mutant-strain-of-yersinia-pestis-vaccine-ev76-offers-enhanced-safety-and-improved-protection-against-plague
#2
JOURNAL ARTICLE
Xiao Guo, Youquan Xin, Zehui Tong, Shiyang Cao, Yuan Zhang, Gengshan Wu, Hongyan Chen, Tong Wang, Yajun Song, Qingwen Zhang, Ruifu Yang, Zongmin Du
We recently identified two virulence-associated small open reading frames (sORF) of Yersinia pestis, named yp1 and yp2, and null mutants of each individual genes were highly attenuated in virulence. Plague vaccine strain EV76 is known for strong reactogenicity, making it not suitable for use in humans. To improve the immune safety of EV76, three mutant strains of EV76, Δyp1, Δyp2, and Δyp1&yp2 were constructed and their virulence attenuation, immunogenicity, and protective efficacy in mice were evaluated...
March 28, 2024: PLoS Pathogens
https://read.qxmd.com/read/38547316/chronic-viral-infection-impairs-immune-memory-to-a-different-pathogen
#3
JOURNAL ARTICLE
Cheng Yang, Zhicui Liu, Ying Yang, Luis J Cocka, Yongguo Li, Weihong Zeng, Hao Shen
Chronic viral infections cause T cell dysfunction in both animal models and human clinical settings, thereby affecting the ability of the host immune system to clear viral pathogens and develop proper virus-specific immune memory. However, the impact of chronic viral infections on the host's immune memory to other pathogens has not been well described. In this study, we immunized mice with recombinant Listeria monocytogenes expressing OVA (Lm-OVA) to generate immunity to Lm and allow analysis of OVA-specific memory T (Tm) cells...
March 28, 2024: PLoS Pathogens
https://read.qxmd.com/read/38547310/the-wolbachia-wale1-effector-alters-drosophila-endocytosis
#4
JOURNAL ARTICLE
MaryAnn Martin, Sergio López-Madrigal, Irene L G Newton
The most common intracellular bacterial infection is Wolbachia pipientis, a microbe that manipulates host reproduction and is used in control of insect vectors. Phenotypes induced by Wolbachia have been studied for decades and range from sperm-egg incompatibility to male killing. How Wolbachia alters host biology is less well understood. Previously, we characterized the first Wolbachia effector-WalE1, which encodes an alpha-synuclein domain at the N terminus. Purified WalE1 sediments with and bundles actin and when heterologously expressed in flies, increases Wolbachia titer in the developing oocyte...
March 28, 2024: PLoS Pathogens
https://read.qxmd.com/read/38547304/characterization-of-a-hemolytic-and-antibiotic-resistant-pseudomonas-aeruginosa-strain-s3-pathogenic-to-fish-isolated-from-mahananda-river-in-india
#5
JOURNAL ARTICLE
Dipanwita Ghosh, Preeti Mangar, Abhinandan Choudhury, Anoop Kumar, Aniruddha Saha, Protip Basu, Dipanwita Saha
Virulent strain Pseudomonas aeruginosa isolated from Mahananda River exhibited the highest hemolytic activity and virulence factors and was pathogenic to fish as clinical signs of hemorrhagic spots, loss of scales, and fin erosions were found. S3 was cytotoxic to the human liver cell line (WRL-68) in the trypan blue dye exclusion assay. Genotype characterization using whole genome analysis showed that S3 was similar to P. aeruginosa PAO1. The draft genome sequence had an estimated length of 62,69,783 bp, a GC content of 66...
2024: PloS One
https://read.qxmd.com/read/38547254/a-clinically-attenuated-double-mutant-of-porcine-reproductive-and-respiratory-syndrome-virus-2-that-does-not-prompt-overexpression-of-proinflammatory-cytokines-during-co-infection-with-a-secondary-pathogen
#6
JOURNAL ARTICLE
Chia-Ming Su, Jineui Kim, Junyu Tang, Yu Fan Hung, Federico A Zuckermann, Robert Husmann, Patrick Roady, Jiyoun Kim, Young-Min Lee, Dongwan Yoo
Porcine reproductive and respiratory syndrome virus (PRRSV) is known to suppress the type I interferon (IFNs-α/β) response during infection. PRRSV also activates the NF-κB signaling pathway, leading to the production of proinflammatory cytokines during infection. In swine farms, co-infections of PRRSV and other secondary bacterial pathogens are common and exacerbate the production of proinflammatory cytokines, contributing to the porcine respiratory disease complex (PRDC) which is clinically a severe disease...
March 28, 2024: PLoS Pathogens
https://read.qxmd.com/read/38547101/behind-the-scenes-centromere-driven-genomic-innovations-in-fungal-pathogens
#7
JOURNAL ARTICLE
Aswathy Narayanan, Md Hashim Reza, Kaustuv Sanyal
No abstract text is available yet for this article.
March 2024: PLoS Pathogens
https://read.qxmd.com/read/38546951/insights-on-the-genomic-diversity-virulence-and-resistance-profile-of-a-campylobacter-jejuni-strain-isolated-from-a-hospitalized-patient-in-brazil
#8
JOURNAL ARTICLE
Carolina Nogueira Gomes, Miliane Rodrigues Frazão, Amanda Aparecida Seribelli, Dillon Oliver Reese Barker, Emily Victoria Che, Mara Corrêa Lelles Nogueira, Eduardo Napoleon Taboada, Juliana Pfrimer Falcão
Campylobacteriosis is currently recognized as one of the major causes of foodborne bacterial diseases worldwide. In Brazil, there is insufficient data to estimate the impact of Campylobacter in public health. The aim of this present study was to characterize a C. jejuni CJ-HBSJRP strain isolated from a hospitalized patient in Brazil by its ability to invade human Caco-2 epithelial cells, to survive in U937 human macrophages, and to assess its phenotypic antimicrobial resistance profile. In addition, prophages, virulence and antimicrobial resistance genes were search using whole-genome sequencing data...
March 28, 2024: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://read.qxmd.com/read/38546929/pd-like-pathogenesis-in-caenorhabditis-elegans-intestinally-infected-with-nocardia-farcinica-and-the-underlying-molecular-mechanisms
#9
JOURNAL ARTICLE
Wenwen Liu, Wenhui Zhou, Peiji Zhao, Tingting Wu, Huan Gu, Yixin Li, Chidi Zhong, Hua Bai, Ninghui Zhao, Xiaowei Huang
Parkinson's disease (PD) is a neurodegenerative disorder characterized by the abnormal aggregation of α-synuclein (α-syn) and the loss of dopaminergic neurons. Although microbial infection has been implicated in the pathogenesis of PD, the associated virulence factors and the underlying molecular mechanisms require further elucidation. Here, we found that intestinal infection with Nocardia farcinica induced a series of PD-like symptoms in Caenorhabditis elegans, such as the accelerated degeneration of dopaminergic neurons, impaired locomotion capacity, and enhanced α-syn aggregation, through the disturbance of mitochondrial functions...
March 28, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38546809/comparative-genetic-and-pathogenic-approaches-of-escherichia-coli-isolated-simultaneously-from-pyometra-and-urine-of-bitches
#10
JOURNAL ARTICLE
Cassiane Elisabete Lopes, Tainara Soares Weyh, Camila Azevedo Moni, Maria Eduarda Dias, Tania Aparecida Tardelli Gomes, Mateus Matiuzzi da Costa, Rommel Thiago Jucá Ramos, Aristóteles Góes-Neto, Vasco Ariston de Carvalho Azevedo, Bertram Brenig, Franciele Maboni Siqueira
Escherichia coli (E. coli) are widely related to pyometra and cystitis in dogs, and these infections can occur simultaneously. The goal of this study was to determine genetic and pathogenic insights of 14 E. coli isolated simultaneously from pyometra content and bladder urine of seven bitches. To achieve this, in silico and in vitro comparative analyses were conducted. Whole-genome comparisons demonstrated that E. coli isolated from pyometra and urine of the same animal were predominantly genetic extraintestinal E...
March 28, 2024: Veterinary Research Communications
https://read.qxmd.com/read/38546267/the-staphylococcus-aureus-regulatory-program-in-a-human-skin-like-environment
#11
JOURNAL ARTICLE
Flavia G Costa, Krista B Mills, Heidi A Crosby, Alexander R Horswill
UNLABELLED: Staphylococcus aureus is a Gram-positive pathogen responsible for the majority of skin and soft tissue infections (SSTIs). S. aureus colonizes the anterior nares of approximately 20%-30% of the population and transiently colonizes the skin, thereby increasing the risk of developing SSTIs and more serious infections. Current laboratory models that mimic the skin surface environment are expensive, require substantial infrastructure, and limit the scope of bacterial physiology studies under human skin conditions...
March 28, 2024: MBio
https://read.qxmd.com/read/38546228/white-opaque-switching-in-candida-albicans-cell-biology-regulation-and-function
#12
REVIEW
David R Soll
SUMMARY Candida albicans remains a major fungal pathogen colonizing humans and opportunistically invading tissue when conditions are predisposing. Part of the success of C. albicans was attributed to its capacity to form hyphae that facilitate tissue invasion. However, in 1987, a second developmental program was discovered, the "white-opaque transition," a high-frequency reversible switching system that impacted most aspects of the physiology, cell architecture, virulence, and gene expression of C. albicans ...
March 28, 2024: Microbiology and Molecular Biology Reviews: MMBR
https://read.qxmd.com/read/38545601/multireceptor-phage-cocktail-against-salmonella-enterica-to-circumvent-phage-resistance
#13
JOURNAL ARTICLE
Carlos E Martinez-Soto, Michael McClelland, Andrew M Kropinski, Janet T Lin, Cezar M Khursigara, Hany Anany
Non-Typhoidal Salmonella (NTS) is one of the most common food-borne pathogens worldwide, with poultry products being the major vehicle for pathogenesis in humans. The use of bacteriophage (phage) cocktails has recently emerged as a novel approach to enhancing food safety. Here, a multireceptor Salmonella phage cocktail of five phages was developed and characterized. The cocktail targets four receptors: O-antigen, BtuB, OmpC, and rough Salmonella strains. Structural analysis indicated that all five phages belong to unique families or subfamilies...
2024: Microlife
https://read.qxmd.com/read/38545168/potential-of-ilh%C3%A3-us-virus-to-emerge
#14
JOURNAL ARTICLE
Kenneth S Plante, Jessica A Plante, Sasha R Azar, Divya P Shinde, Dionna Scharton, Alice F Versiani, Natalia Ingrid Oliveira da Silva, Taylor Strange, Lívia Sacchetto, Eric B Fokam, Shannan L Rossi, Scott C Weaver, Rafael E Marques, Mauricio L Nogueira, Nikos Vasilakis
Ilhéus virus (ILHV)( Flaviviridae:Orthoflavivirus ) is an arthropod-borne virus (arbovirus) endemic to Central and South America and the Caribbean. First isolated in 1944, most of our knowledge derives from surveillance and seroprevalence studies. These efforts have detected ILHV in a broad range of mosquito and vertebrate species, including humans, but laboratory investigations of pathogenesis and vector competence have been lacking. Here, we develop an immune intact murine model with several ages and routes of administration...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38545156/binucleate-rhizoctonia-induced-tomato-resistance-against-rhizoctonia-solani-via-affecting-antioxidants-and-cell-wall-reinforcement
#15
JOURNAL ARTICLE
Parissa Taheri, Fatemeh Hosseini-Zahani, Saeed Tarighi
Isolates of Rhizoctonia solani (AG-3 PT, AG-4 HG-I, AG-4 HG-II) and one binucleate Rhizoctonia sp. (BNR) belonging to AG-Bb were investigated for pathogenicity on tomato cultivar Mobil. The BNR isolate revealed the lowest virulence and it was used as biocontrol agent against R. solani AG-4 HG-II, which showed the highest virulence on tomato. Inoculation of tomato plants with the hypovirulent BNR isolate reduced the disease symptoms of R. solani and induced resistance. Resistance induction was observed not only on the plants simultaneously inoculated with BNR and R...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38544857/mapping-the-trna-modification-landscape-of-bartonella-henselae-houston-i-and-bartonella-quintana-toulouse
#16
JOURNAL ARTICLE
Samia Quaiyum, Jingjing Sun, Virginie Marchand, Guangxin Sun, Colbie J Reed, Yuri Motorin, Peter C Dedon, Michael F Minnick, Valérie de Crécy-Lagard
Transfer RNA (tRNA) modifications play a crucial role in maintaining translational fidelity and efficiency, and they may function as regulatory elements in stress response and virulence. Despite their pivotal roles, a comprehensive mapping of tRNA modifications and their associated synthesis genes is still limited, with a predominant focus on free-living bacteria. In this study, we employed a multidisciplinary approach, incorporating comparative genomics, mass spectrometry, and next-generation sequencing, to predict the set of tRNA modification genes responsible for tRNA maturation in two intracellular pathogens- Bartonella henselae Houston I and Bartonella quintana Toulouse, which are causative agents of cat-scratch disease and trench fever, respectively...
2024: Frontiers in Microbiology
https://read.qxmd.com/read/38544742/silver-nanoparticles-synthesized-from-pseudomonas-aeruginosa-pyoverdine-antibiofilm-and-antivirulence-agents
#17
JOURNAL ARTICLE
Nazia Tabassum, Fazlurrahman Khan, Geum-Jae Jeong, Du-Min Jo, Young-Mog Kim
The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one of the major issues in the healthcare sector. In the present study, nanomaterial-based controlling the biofilm and virulence properties has been considered an alternative approach. Pyoverdine (PVD) isolated from the Pseudomonas aeruginosa was utilized as a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful in a targeted action to microbial pathogens due to the recognition of the corona of the nanoparticles by the pathogenic membrane...
June 2024: Biofilm
https://read.qxmd.com/read/38544725/description-of-the-covid-19-epidemiology-in-malaysia
#18
JOURNAL ARTICLE
Mohamad Nadzmi Md Nadzri, Ahmed Syahmi Syafiq Md Zamri, Sarbhan Singh, Mohd Ghazali Sumarni, Chee Herng Lai, Cia Vei Tan, Tahir Aris, Hishamshah Mohd Ibrahim, Balvinder Singh Gill, Nur'Ain Mohd Ghazali, Nuur Hafizah Md Iderus, Mei Cheng Lim, Lonny Chen Rong Qi Ahmad, Mohd Kamarulariffin Kamarudin, Nur Ar Rabiah Ahmad, Kok Keng Tee, Asrul Anuar Zulkifli
INTRODUCTION: Since the COVID-19 pandemic began, it has spread rapidly across the world and has resulted in recurrent outbreaks. This study aims to describe the COVID-19 epidemiology in terms of COVID-19 cases, deaths, ICU admissions, ventilator requirements, testing, incidence rate, death rate, case fatality rate (CFR) and test positivity rate for each outbreak from the beginning of the pandemic in 2020 till endemicity of COVID-19 in 2022 in Malaysia. METHODS: Data was sourced from the GitHub repository and the Ministry of Health's official COVID-19 website...
2024: Frontiers in Public Health
https://read.qxmd.com/read/38544640/molecular-characterization-of-the-glya-gene-from-the-clinical-isolates-of-tannerella-forsythia
#19
JOURNAL ARTICLE
Sujitha A, A S Smiline Girija, Vijayashree J Priyadharsini
BACKGROUND: The  glyA gene in Tannerella forsythia is attributed for its virulence by producing the enzyme serine hydroxymethyltransferase (SHMT), which plays a vital role in bacterial cell metabolism. OBJECTIVES: The study is thus aimed to determine the frequency of the glyA gene from the clinical strains of T. forsythia isolated from periodontitis patients. MATERIALS AND METHODS: Forty-five patients with varying degrees of periodontitis were included in the study, and the plaque samples collected from them were anaerobically processed by inoculating onto sterile anaerobic blood agar plates using a gaspak system, with incubation at 37°C for 5-7 days...
February 2024: Curēus
https://read.qxmd.com/read/38544331/the-impact-of-indole-and-mucin-on-sporulation-biofilm-formation-and-enterotoxin-production-in-foodborne-clostridium-perfringens
#20
JOURNAL ARTICLE
Chao Wang, Tom Defoirdt, Andreja Rajkovic
AIMS: Indole and mucin are compounds found in the host environment as they are produced by the host or by the host-associated microbiota. This study investigated whether indole and mucin impact C. perfringens growth and sporulation, as well as enterotoxin production and biofilm formation. METHODS AND RESULTS: There was no impact on growth of C. perfringens for up to 400 µM indole and 240 mg/L mucin, and neither indole nor mucin affected sporulation. Reverse-transcriptase qPCR showed that mucin strongly upregulated the expression of C...
March 27, 2024: Journal of Applied Microbiology
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