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https://www.readbyqxmd.com/read/28448526/human-alkaline-phosphatase-dephosphorylates-microbial-products-and-is-elevated-in-preterm-neonates-with-a-history-of-late-onset-sepsis
#1
Matthew Pettengill, Juan D Matute, Megan Tresenriter, Julie Hibbert, David Burgner, Peter Richmond, José Luis Millán, Al Ozonoff, Tobias Strunk, Andrew Currie, Ofer Levy
BACKGROUND: A host defense function for Alkaline phosphatases (ALPs) is suggested by the contribution of intestinal ALP to detoxifying bacterial lipopolysaccharide (endotoxin) in animal models in vivo and the elevation of ALP activity following treatment of human cells with inflammatory stimuli in vitro. However the activity of ALP in human plasma (primarily tissue-nonspecific ALP; TNAP) on lipopolysaccharide and other microbial products has not been assessed, nor has its expression been studied in preterm newborns, a vulnerable population at high risk of sepsis...
2017: PloS One
https://www.readbyqxmd.com/read/28447329/immune-response-against-salmonella-enteritidis-is-unsettled-by-hiv-infection
#2
Maira Costa Cacemiro, Milena Sobral Espíndola, Leonardo Judson Galvão-Lima, Luana Silva Soares, Caroline Fontanari, Marco Aurélio Prata, Fábio Campioni, Juliana Pfrimer Falcão, Valdes Roberto Bollela, Fabiani Gai Frantz
The human immunodeficiency virus (HIV) is responsible for more than 2 million new infections per year and opportunistic infections such as Salmonella spp. Gastroenteritis is an important cause of mortality and morbidity in developing countries. Monocytes and macrophages play a critical role in the innate immune response against bacterial infections. However during HIV infection the virus can infect these cells and although they are more resistant to the cytopathic effects, they represent an important viral reservoir in these patients...
April 27, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28445250/enteric-fever-in-children-in-western-sydney-australia-2003-2015
#3
Ameneh Khatami, Fouzia Khan, Kristine K Macartney
BACKGROUND: Enteric fever is a vaccine-preventable disease with cases in Australia predominantly acquired overseas. The aim of this study was to define the burden of enteric fever in children presenting to a pediatric hospital in Western Sydney between 2003 and 2015. METHODS: Cases between January 2003 and December 2013 were ascertained through medical records using ICD-coded discharge diagnoses, cross-referenced with microbiology laboratory data for all isolates of Salmonella enterica serovar Typhi (S...
April 25, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28445131/therapeutic-benefit-of-salmonella-attributed-to-lps-and-tnf-%C3%AE-is-exhaustible-and-dictated-by-tumor-susceptibility
#4
Dino Kocijancic, Sara Leschner, Sebastian Felgner, Ronja-Melinda Komoll, Michael Frahm, Vinay Pawar, Siegfried Weiss
The potential of bacteria-mediated tumor therapy (BMTT) is highlighted by more than a century of investigation. Attenuated Salmonella has prevailed as promising therapeutic agents. For BMTT - categorized as an immune therapy - the exact contribution of particular immune reactions to the therapeutic effect remains ambiguous. In addition, one could argue for or against the requirement of bacterial viability and tumor targeting. Herein we evaluate the isolated therapeutic efficacy of purified LPS and TNF-α, which together account for a dominant immunogenic pathway of gram negative bacteria like Salmonella...
April 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28444752/prevalence-and-pathogen-load-of-campylobacter-spp-salmonella-enterica-and-escherichia-coli-o157-o145-serogroup-in-sheep-faeces-collected-at-sale-yards-and-in-abattoir-effluent-in-western-australia
#5
R Yang, S Abraham, G E Gardner, U Ryan, C Jacobson
OBJECTIVE: Develop a multiplex quantitative PCR assay to investigate the prevalence and shedding of Escherichia coli O157/O145, Salmonella spp. and Campylobacter spp. in sheep at sale yards and abattoirs. METHODS: A qPCR for E. coli O157/O145 was developed, validated and multiplexed with an existing qPCR for Campylobacter and Salmonella enterica. The absolute numbers of E. coli O157/O145, Campylobacter and Salmonella in control samples was determined using droplet digital PCR...
May 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28444375/evaluation-of-the-alpha-amylase-activity-as-an-indicator-of-pasteurization-efficiency-and-microbiological-quality-of-liquid-whole-eggs
#6
Guilherme Resende da Silva, Liliane Denize Miranda Menezes, Isabela Pereira Lanza, Daniela Duarte de Oliveira, Carla Aparecida Silva, Roger Wilker Tavares Klein, Débora Cristina Sampaio de Assis, Silvana de Vasconcelos Cançado
In order to evaluate the efficiency of the pasteurization process in liquid whole eggs, an UV/visible spectrophotometric method was developed and validated for the assessment of alpha-amylase activity. Samples were collected from 30 lots of raw eggs (n = 30) and divided into three groups: one was reserved for analysis of the raw eggs, the second group was pasteurized at 61.1°C for 3.5 minutes (n = 30), and the third group was pasteurized at 64.4°C for 2.5 minutes (n = 30). In addition to assessing alpha-amylase activity, the microbiological quality of the samples was also evaluated by counting total and thermotolerant coliforms, mesophilic aerobic microorganisms, Staphylococcus spp...
April 24, 2017: Poultry Science
https://www.readbyqxmd.com/read/28444242/virulence-and-antimicrobial-resistance-of-salmonella-spp-and-escherichia-coli-in-the-beef-jerky-production-line
#7
Fernanda Pereira Fernandes, Flávia Liége Schütz Voloski, Tassiana Ramires, Louise Haubert, Giulia Giugliani Reta, Rafael Gianella Mondadori, Wladimir Padilha da Silva, Rita de Cássia, Santos da Conceição, Eduarda Hallal Duval
Intense manipulation during beef jerky production increases the possibility of contamination with pathogenic microorganisms. This study evaluated the contamination by thermotolerant coliforms, Escherichia coli and Salmonella spp. on processing surfaces and raw materials during beef jerky production, as well as in the final product. Thermotolerant coliforms were found on all surfaces tested and in the raw material. Escherichia coli was identified in 6.7% of the surface samples, while Salmonella spp. was found in 3...
April 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28443633/corrigendum-characterization-of-a-p1-like-bacteriophage-carrying-ctx-m-27-in-salmonella-spp-resistant-to-third-generation-cephalosporins-isolated-from-pork-in-china
#8
Ling Yang, Wan Li, Gui-Ze Jiang, Wen-Hui Zhang, Huan-Zhong Ding, Ya-Hong Liu, Zhen-Ling Zeng, Hong-Xia Jiang
No abstract text is available yet for this article.
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28443586/salmonella-enterica-serovar-enteritidis-liver-abscess-after-blunt-abdominal-trauma
#9
Agata Ladic, Igor Petrovic, Ante Gojevic, Emil Kinda, Ivo Coza
BACKGROUND: Salmonella enterica serovar Enteritidis is among the most reported serotypes of Salmonella species worldwide, but rarely reported as the causative agent of a liver abscess. CASE REPORT: We present a patient with an abdominal blunt trauma. An initial abdominal CT scan revealed rupture of the right kidney and of the liver. Two days later, his physical state deteriorated and a new CT scan was obtained. An eight-centimetre large liver abscess was verified...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28443537/the-legacy-of-genetic-analysis-advances-contemporary-research-with-escherichia-coli-k-12-and-salmonella-enterica-serovar-typhimurium-lt2
#10
Valley Stewart
Escherichia coli K-12 and Salmonella enterica serovar Typhimurium LT2 became standard organisms for genetic analysis during the Truman administration. Half a century later, genetic analysis with these strains had become an art form, interpreted through 23 articles in the ambitious two-volume masterpiece edited by the late Fred Neidhardt and colleagues. These legacy articles now are available through EcoSal Plus, so as to inform and inspire contemporary genetic analyses in these standard organisms and their relatives...
April 2017: EcoSal Plus
https://www.readbyqxmd.com/read/28443289/frequency-and-duration-of-fecal-shedding-of-salmonella-enteritidis-by-experimentally-infected-laying-hens-housed-in-enriched-colony-cages-at-different-stocking-densities
#11
Richard K Gast, Rupa Guraya, Deana R Jones, Kenneth E Anderson, Darrin M Karcher
Human infections with Salmonella Enteritidis are often attributed to the consumption of contaminated eggs, so the prevalence of this pathogen in egg-laying poultry is an important public health risk factor. Numerous and complex environmental influences on Salmonella persistence and transmission are exerted by management practices and housing facilities used in commercial egg production. In recent years, the animal welfare implications of poultry housing systems have guided the development of alternatives to traditional cage-based housing, but their food safety consequences are not yet fully understood...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28443246/genetic-and-dietary-iron-overload-differentially-affect-the-course-of-salmonella-typhimurium-infection
#12
Manfred Nairz, Andrea Schroll, David Haschka, Stefanie Dichtl, Piotr Tymoszuk, Egon Demetz, Patrizia Moser, Hubertus Haas, Ferric C Fang, Igor Theurl, Günter Weiss
Genetic and dietary forms of iron overload have distinctive clinical and pathophysiological features. HFE-associated hereditary hemochromatosis is characterized by overwhelming intestinal iron absorption, parenchymal iron deposition, and macrophage iron depletion. In contrast, excessive dietary iron intake results in iron deposition in macrophages. However, the functional consequences of genetic and dietary iron overload for the control of microbes are incompletely understood. Using Hfe(+/+) and Hfe(-/-) mice in combination with oral iron overload in a model of Salmonella enterica serovar Typhimurium infection, we found animals of either genotype to induce hepcidin antimicrobial peptide expression and hypoferremia following systemic infection in an Hfe-independent manner...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28443224/the-effect-of-lactobacillus-acidophilus-ptcc-1643-on-cultured-intestinal-epithelial-cells-infected-with-salmonella-enterica-serovar-enteritidis
#13
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
OBJECTIVES: Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28443073/feed-supplementation-with-red-seaweeds-chondrus-crispus-and-sarcodiotheca-gaudichaudii-reduce-salmonella-enteritidis-in-laying-hens
#14
Garima Kulshreshtha, Bruce Rathgeber, Janice MacIsaac, Martine Boulianne, Lehoux Brigitte, Glenn Stratton, Nikhil A Thomas, Alan T Critchley, Jeff Hafting, Balakrishnan Prithiviraj
Salmonella Enteritidis is vertically transmitted to eggs from laying hens through infected ovaries and oviducts. S. Enteritidis can also penetrate the eggshell from contaminated feces. Reducing S. Enteritidis in laying hens is vital to provide safer eggs and minimize the spread of salmonellosis to humans. Antibiotics have been widely used to control bacterial diseases in broilers and laying hens. However, there is a major concern that the use of antibiotics leads to the development of antibiotic resistance and adverse effects on microbiota of the treated birds...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28443068/evaluation-of-the-probiotic-strain-bifidobacterium-longum-subsp-infantis-cect-7210-capacities-to-improve-health-status-and-fight-digestive-pathogens-in-a-piglet-model
#15
Emili Barba-Vidal, Lorena Castillejos, Paola López-Colom, Montserrat Rivero Urgell, José A Moreno Muñoz, Susana M Martín-Orúe
Probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections. The objective of this study is to demonstrate the potential of the probiotic strain Bifidobacterium longum subsp. infantis CECT 7210 (B. infantis IM1) to prevent and fight intestinal disease by using a Salmonella Typhimurium (Trial 1) or an enterotoxigenic Escherichia coli K88 (Trial 2) oral challenge in a weaning piglet model. Seventy-two piglets were used in each trial. After an adaptation period, animals were orally challenged...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28441599/transport-and-attenuation-of-salmonella-enterica-fecal-indicator-bacteria-and-a-poultry-litter-marker-gene-are-correlated-in-soil-columns
#16
Sirisha Mantha, Angela Anderson, Saraswati Poudel Acharya, Valerie J Harwood, Jennifer Weidhaas
Millions of tons of fecal-contaminated poultry litter are applied to U.S. agricultural fields annually. Precipitation and irrigation facilitate transport of fecal-derived pathogens and fecal indicator bacteria (FIB) to groundwater. The goal of this study was to compare transport of pathogens, FIB, and a microbial source tracking marker gene for poultry litter (LA35) in a simulated soil-to-groundwater system. Nine laboratory soil columns containing four different soil types were used to evaluate microbial transport to groundwater via infiltration...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28440198/the-role-of-neutrophil-proteases-in-lps-induced-production-of-regulatory-rheumatoid-factor-that-suppresses-autoimmunity
#17
Elena Stolyarova, Liubov Beduleva, Alexandr Sidorov, Igor Menshikov, Alexey Terentiev, Kseniya Vachrusheva
BACKGROUND: We recently identified a regulatory rheumatoid factor (regRF), the production of which is associated with autoimmune disease resistance and remission. In studies of regRF in the blood of healthy rats, spontaneous increases in the regRF level were noted. We suggest that in the normal state, a mechanism exists for maintaining the activity of the pool of regRF-producing lymphocytes at a level that makes it possible to control the expansion of autoreactive lymphocytes. OBJECTIVE: The purpose of this study was to test the hypothesis that the endogenous stimulator of regRF production is Fc fragments of IgG that are formed upon exposure to the proteases of neutrophils...
April 24, 2017: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/28439072/fecal-microbial-characterization-of-hospitalized-patients-with-suspected-infectious-diarrhea-shows-significant-dysbiosis
#18
Tzipi Braun, Ayelet Di Segni, Marina BenShoshan, Roy Asaf, James E Squires, Sarit Farage Barhom, Efrat Glick Saar, Karen Cesarkas, Gill Smollan, Batia Weiss, Sharon Amit, Nathan Keller, Yael Haberman
Hospitalized patients are at increased risk for acquiring healthcare-associated infections (HAIs) and inadequate nutrition. The human intestinal microbiota plays vital functions in nutrient supply and protection from pathogens, yet characterization of the microbiota of hospitalized patients is lacking. We used 16S rRNA amplicon sequencing to characterize the global pattern of microbial composition of fecal samples from 196 hospitalized patients with suspected infectious diarrhea in comparison to healthy, non-hospitalized subjects (n = 881), and to traditional culture results...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28439039/invs-coordinates-expression-of-prgh-and-fimz-and-is-required-for-invasion-of-epithelial-cells-by-salmonella-enterica-serovar-typhimurium
#19
Lu Wang, Xia Cai, Shuyan Wu, Rajdeep Bomjan, Ernesto S Nakayasu, Kristian Händler, Jay C D Hinton, Daoguo Zhou
Deep sequencing has revolutionized our understanding of the bacterial RNA world, and has facilitated the identification of 280 sRNAs in Salmonella Despite suspicions that sRNAs may play important roles in Salmonella pathogenesis, the functions of most sRNAs remain unknown. To advance our understanding of RNA biology in Salmonella virulence, we searched for sRNAs required for bacterial invasion into non-phagocytic cells. After screening 75 sRNAs, we discovered that ablation of InvS caused a significant decrease of Salmonella invasion into epithelial cells...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438393/use-of-the-entericbio-gastro-panel-ii-in-a-diagnostic-microbiology-laboratory-challenges-and-opportunities
#20
Gary McAuliffe, Liselle Bissessor, Deborah Williamson, Sharon Moore, Janet Wilson, Muriel Dufour, Susan Taylor, Arlo Upton
We sought to verify the performance of the EntericBio real-time Gastro Panel 2 (GP2; Serosep, Ireland) assay targeting Salmonella, Shigella, Campylobacter, verotoxin producing E. coli (VTEC), Giardia, and Cryptosporidium in a high-throughput diagnostic laboratory. Following a technical verification period where 237 faecal samples were tested in parallel, guided culture and discrepancy analysis were performed. A total of 13,223 faecal samples were screened by the GP2 assay. During technical verification, sensitivity, specificity, negative and positive predictive value of the GP2 assay compared with conventional methods were 100% (83-100%), 98% (96...
April 21, 2017: Pathology
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