keyword
https://read.qxmd.com/read/38464616/anti-listerial-peptides-from-a-marine-bacillus-velezensis-ftl7-production-optimization-characterizations-and-molecular-docking-studies
#21
JOURNAL ARTICLE
Lidiya C Johny, B S Gnanesh Kumar, S J Aditya Rao, P V Suresh
UNLABELLED: Antimicrobial peptides (AMPs) with potent anti-listerial activity were characterized from a novel marine Bacillus velezensis FTL7. A Box-Behnken statistical experimental design was used to study the combined impact of culture conditions on the production of AMPs by B. velezensis FTL7. The conditions optimized by statistical experimental design were 34.5 °C incubation temperature, 23 h incubation time, and 7.6 initial pH of the medium. AMP purification was performed by ammonium sulphate fractionation and butanol extraction followed by reversed-phase C18 solid-phase extraction...
April 2024: 3 Biotech
https://read.qxmd.com/read/38457439/molecular-crowding-alters-the-interactions-of-polymyxin-lipopeptides-within-the-periplasm-of-e-coli-insights-from-molecular-dynamics
#22
JOURNAL ARTICLE
Iain P S Smith, Conrado Pedebos, Syma Khalid
The cell envelope of Gram-negative bacteria is a crowded tripartite architecture that separates the cell interior from the external environment. Two membranes encapsulate the aqueous periplasm, which contains the cell wall. Little is known about the mechanisms via which antimicrobial peptides move through the periplasm from the outer membrane to their site of action, the inner membrane. We utilize all-atom molecular dynamics to study two antimicrobial peptides, polymyxins B1 and E, within models of the E. coli periplasm crowded to different extents...
March 8, 2024: Journal of Physical Chemistry. B
https://read.qxmd.com/read/38453942/genomic-insights-and-anti-phytopathogenic-potential-of-siderophore-metabolome-of-endolithic-nocardia-mangyaensis-nh1
#23
JOURNAL ARTICLE
Irina V Khilyas, Maria I Markelova, Liia R Valeeva, Tatiana M Ivoilova, Elena Shagimardanova, Alexander V Laikov, Anna A Elistratova, Ekaterina S Berkutova, Guenter Lochnit, Margarita R Sharipova
Actinobacteria are one of the predominant groups that successfully colonize and survive in various aquatic, terrestrial and rhizhospheric ecosystems. Among actinobacteria, Nocardia is one of the most important agricultural and industrial bacteria. Screening and isolation of Nocardia related bacteria from extreme habitats such as endolithic environments are beneficial for practical applications in agricultural and environmental biotechnology. In this work, bioinformatics analysis revealed that a novel strain Nocardia mangyaensis NH1 has the capacity to produce structurally varied bioactive compounds, which encoded by non-ribosomal peptide synthases (NRPS), polyketide synthase (PKS), and post-translationally modified peptides (RiPPs)...
March 7, 2024: Scientific Reports
https://read.qxmd.com/read/38452914/polyurethane-degradation-by-extracellular-urethanase-producing-bacterial-isolate-moraxella-catarrhalis-strain-bmpps3
#24
JOURNAL ARTICLE
Maheswaran B, Sebastin Raj J, Pandiyarajan P, Jaya Santhi R, Mythili R, Vignesh K S, Woong Kim, Karmegam N, Govarthanan Muthusamy
Plastic waste has become a global issue and a threat to the ecosystem. The present study isolated polyurethane (PU) degrading bacterial species from soil dumped with plastic wastes. Four bacterial isolates, RS1, RS6, RS9 and RS13 were obtained and their ability to degrade PU in a synthetic medium with PU as a sole source of carbon was assessed individually. After thirty days of incubation, the highest PU weight loss of 67.36 ± 0.32% was recorded in the medium containing RS13 isolate. The results of FTIR revealed the occurrence of carbonyl peaks...
March 5, 2024: Environmental Research
https://read.qxmd.com/read/38451229/antifungal-potential-of-lipopeptides-produced-by-the-bacillus-siamensis-sh420-strain-against-fusarium-graminearum
#25
JOURNAL ARTICLE
Sarfaraz Hussain, Bowen Tai, Maratab Ali, Israt Jahan, Suha Sakina, Gang Wang, Xinlong Zhang, Yixuan Yin, Fuguo Xing
Biological control is a more sustainable and environmentally friendly alternative to chemical fungicides for controlling Fusarium spp. infestations. In this work, Bacillus siamensis Sh420 isolated from wheat rhizosphere showed a high antifungal activity against Fusarium graminearum as a secure substitute for fungicides. Sh420 was identified as B. siamensis using phenotypic evaluation and 16S rDNA gene sequence analysis. An in vitro antagonistic study showed that Sh420's lipopeptide (LP) extract exhibited strong antifungal properties and effectively combated F...
March 7, 2024: Microbiology Spectrum
https://read.qxmd.com/read/38438885/a-synthetic-multivalent-lipopeptide-derived-from-pam3csk4-with-irreversible-influenza-inhibition-and-immuno-stimulating-effects
#26
JOURNAL ARTICLE
Yong Zhu, Lixia Wei, Arnaud Charles-Antoine Zwygart, Pablo Gaínza, Quy Ong Khac, Francesca Olgiati, Armand Kurum, Li Tang, Bruno Correia, Caroline Tapparel, Francesco Stellacci
The activation of the host adaptive immune system is crucial for eliminating viruses. However, influenza infection often suppresses the innate immune response that precedes adaptive immunity, and the adaptive immune responses are typically delayed. Dendritic cells, serving as professional antigen-presenting cells, have a vital role in initiating the adaptive immune response. In this study, an immuno-stimulating antiviral system (ISAS) is introduced, which is composed of the immuno-stimulating adjuvant lipopeptide Pam3CSK4 that acts as a scaffold onto which it is covalently bound 3 to 4 influenza-inhibiting peptides...
March 4, 2024: Small
https://read.qxmd.com/read/38437465/biocontrol-and-growth-promotion-potential-of-bacillus-subtilis-ctxw-7-6-2-against-rhizoctonia-solani-that-causes-tobacco-target-spot-disease
#27
JOURNAL ARTICLE
Ning Huang, Xin Jin, Jin-Tao Wen, Yi-Fei Zhang, Xu Yang, Guang-Yu Wei, Yi-Kun Wang, Min Qin
Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani , its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of R...
March 1, 2024: Polish Journal of Microbiology
https://read.qxmd.com/read/38431332/inhibition-mechanism-of-crude-lipopeptide-from-bacillus-subtilis-against-aeromonas-veronii-growth-biofilm-formation-and-spoilage-of-channel-catfish-flesh
#28
JOURNAL ARTICLE
Yali Li, Tianqi Wangjiang, Zhida Sun, Liu Shi, Sheng Chen, Lang Chen, Xiaojia Guo, Wenjin Wu, Guangquan Xiong, Lan Wang
Aeromonas veronii is associated with food spoilage and some human diseases, such as diarrhea, gastroenteritis, hemorrhagic septicemia or asymptomatic and even death. This research investigated the mechanism of the growth, biofilm formation, virulence, stress resistance, and spoilage potential of Bacillus subtilis lipopeptide against Aeromonas veronii. Lipopeptides suppressed the transmembrane transport of Aeromonas veronii by changing the cell membrane's permeability, the structure of membrane proteins, and Na+ /K+ -ATPase...
June 2024: Food Microbiology
https://read.qxmd.com/read/38427690/hyperglycaemia-amplifies-tlr-mediated-inflammatory-response-of-m-il4-macrophages-to-dyslipidemic-ligands
#29
JOURNAL ARTICLE
Luis Ernesto Badillo-Garcia, Quan Liu, Kim Ziebner, Michael Balduff, Tatyana Sevastyanova, Christina Schmuttermaier, Harald Klüter, Martin Harmsen, Julia Kzhyshkowska
Hyperglycaemia is critical for initiation of diabetic vascular complications. We systemically addressed the role of hyperglycaemia in the regulation of TLRs in primary human macrophages. Expression of TLRs (1-9) was examined in monocyte-derived M(NC), M(IFNγ) and M(IL4) differentiated in normoglycemic and hyperglycaemic conditions. Hyperglycaemia increased expression of TLR1 and TLR8 in M(NC), TLR 2 and 6 in M(IFNγ), and TLR4 and TLR5 in M(IL4). The strongest effect of hyperglycaemia in M(IL4) was the upregulation of TLR4 gene and protein expression...
March 1, 2024: Journal of Leukocyte Biology
https://read.qxmd.com/read/38425482/%C3%AE-glucans-from-agaricus-bisporus-mushroom-products-drive-trained-immunity
#30
JOURNAL ARTICLE
Sarah Case, Tara O'Brien, Anna E Ledwith, Shilong Chen, Cian J H Horneck Johnston, Emer E Hackett, Michele O'Sullivan, Hugo Charles-Messance, Elaine Dempsey, Supriya Yadav, Jude Wilson, Sinead C Corr, Shipra Nagar, Frederick J Sheedy
INTRODUCTION: Macrofungi, such as edible mushrooms, have been used as a valuable medical resource for millennia as a result of their antibacterial and immuno-modulatory components. Mushrooms contain dietary fibers known as β-glucans, a class of polysaccharides previously linked to the induction of Trained Immunity. However, little is known about the ability of mushroom-derived β-glucans to induce Trained Immunity. METHODS & RESULTS: Using various powdered forms of the white button mushroom (Agaricus bisporus), we found that mouse macrophages pre-treated with whole mushroom powder (WMP) displayed enhanced responses to restimulation with TLR ligands, being particularly sensitive to Toll-like receptor (TLR)-2 stimulation using synthetic lipopeptides...
2024: Frontiers in Nutrition
https://read.qxmd.com/read/38399742/a-2000-year-old-bacillus-stercoris-strain-sheds-light-on-the-evolution-of-cyclic-antimicrobial-lipopeptide-synthesis
#31
JOURNAL ARTICLE
Bessem Chouaia, Jessica Dittmer
Some bacteria (notably the genera Bacillus and Clostridium ) have the capacity to form endospores that can survive for millions of years in isolated habitats. The genomes of such ancient bacteria provide unique opportunities to understand bacterial evolution and metabolic capabilities over longer time scales. Herein, we sequenced the genome of a 2000-year-old bacterial strain (Mal05) isolated from intact apple seeds recovered during archaeological excavations of a Roman villa in Italy. Phylogenomic analyses revealed that this strain belongs to the species Bacillus stercoris and that it is placed in an early-branching position compared to most other strains of this species...
February 6, 2024: Microorganisms
https://read.qxmd.com/read/38399699/genome-sequencing-and-characterization-of-bacillus-velezensis-n23-as-biocontrol-agent-against-plant-pathogens
#32
JOURNAL ARTICLE
Panlei Yang, Qingchao Zeng, Wenxiao Jiang, Luotao Wang, Jie Zhang, Zhenshuo Wang, Qi Wang, Yan Li
The overuse of chemical fungicides against fungal pathogens adversely affects soil and plant health, resulting in environmental problems and food safety. Therefore, biocontrol is considered as an environmentally friendly and cost-effective green technique in environmental protection and agricultural production. We obtained a bacterial strain N23 from a contaminated plate which showed significant inhibition to anthracnose. The strain N23 was identified as Bacillus velezensis based on 16S rRNA gene, gyrA gene, and whole-genome sequence...
January 30, 2024: Microorganisms
https://read.qxmd.com/read/38399458/2023-fda-tides-peptides-and-oligonucleotides-harvest
#33
REVIEW
Danah Al Shaer, Othman Al Musaimi, Fernando Albericio, Beatriz G de la Torre
A total of nine TIDES (pepTIDES and oligonucleoTIDES) were approved by the FDA during 2023. The four approved oligonucleotides are indicated for various types of disorders, including amyotrophic lateral sclerosis, geographic atrophy, primary hyperoxaluria type 1, and polyneuropathy of hereditary transthyretin-mediated amyloidosis. All oligonucleotides show chemically modified structures to enhance their stability and therapeutic effectiveness as antisense or aptamer oligomers. Some of them demonstrate various types of conjugation to driving ligands...
February 13, 2024: Pharmaceuticals
https://read.qxmd.com/read/38393614/a-fluorous-peptide-amphiphile-with-potent-antimicrobial-activity-for-the-treatment-of-mrsa-induced-sepsis-and-chronic-wound-infection
#34
JOURNAL ARTICLE
Jingjing Hu, Nan Liu, Qianqian Fan, Yunqing Gu, Sijia Chen, Fang Zhu, Yiyun Cheng
The rising prevalence of global antibiotic resistance evokes the urgent need for novel antimicrobial candidates. Cationic lipopeptides have attracted much attention due to their strong antimicrobial activity, broad-spectrum and low resistance tendency. Herein, a library of fluoro-lipopeptide amphiphiles was synthesized by tagging a series of cationic oligopeptides with a fluoroalkyl tail via a disulfide spacer. Among the lipopeptide candidates, R6F bearing six arginine moieties and a fluorous tag shows the highest antibacterial activity, and it exhibits an interesting fluorine effect as compared to the non-fluorinated lipopeptides...
February 23, 2024: Angewandte Chemie
https://read.qxmd.com/read/38393143/crude-lipopeptides-produced-by-bacillus-amyloliquefaciens-could-control-the-growth-of-alternaria-alternata-and-production-of-alternaria-toxins-in-processing-tomato
#35
JOURNAL ARTICLE
Yuanyuan Zhang, Yingying Fan, Yingying Dai, Qinlan Jia, Ying Guo, Peicheng Wang, Tingting Shen, Yan Wang, Fengjuan Liu, Wanhui Guo, Aibo Wu, Ziwei Jiao, Cheng Wang
Alternaria spp. and its toxins are the main contaminants in processing tomato. Based on our earlier research, the current study looked into the anti-fungal capacity of crude lipopeptides from B. amyloliquefaciens XJ-BV2007 against A. alternata . We found that the crude lipopeptides significantly inhibited A. alternata growth and reduced tomato black spot disease incidence. SEM analysis found that the crude lipopeptides could change the morphology of mycelium and spores of A. alternata . Four main Alternaria toxins were detected using UPLC-MS/MS, and the findings demonstrated that the crude lipopeptides could lessen the accumulation of Alternaria toxins in vivo and in vitro...
January 25, 2024: Toxins
https://read.qxmd.com/read/38389225/lipopeptides-from-bacillus-velezensis-induced-apoptosis-like-cell-death-in-the-pathogenic-fungus-fusarium-concentricum
#36
JOURNAL ARTICLE
Meichun Chen, Yingjie Deng, Meixia Zheng, Rongfeng Xiao, Xun Wang, Bo Liu, Jin He, Jieping Wang
AIMS: Stem rot caused by Fusarium concentricum is a new disease of Paris polyphylla reported by our research group. The present study investigates the growth inhibitory and apoptotic effects of Bacillus velezensis FJAT-54560 lipopeptide against F. concentricum. METHODS AND RESULTS: HPLC preparation and LC-MS analysis results show that the crude lipopeptides secreted by Bacillus velezensis FJAT-54560 isolated from Jasminum sambac consist of C14-17 iturin A, C14 fengycin B, C16 fengycin A/A2, C18 fengycin A, C20 fengycin B2, C21 fengycin A2, C22-23 fengycin A, C12-16 surfactin A, and C15 surfactin A derivatives...
February 22, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38387185/cholesterol-as-an-inbuilt-immunoadjuvant-for-a-lipopeptide-vaccine-against-group-a-streptococcus-infection
#37
JOURNAL ARTICLE
Nedaa Alharbi, Ahmed O Shalash, Prashamsa Koirala, Jennifer C Boer, Waleed M Hussein, Zeinab G Khalil, Robert J Capon, Magdalena Plebanski, Istvan Toth, Mariusz Skwarczynski
Peptide-based vaccines can trigger highly specific immune responses, although peptides alone are usually unable to confer strong humoral or cellular immunity. Consequently, peptide antigens are administered with immunostimulatory adjuvants, but only a few are safe and effective for human use. To overcome this obstacle, herein a peptide antigen was lipidated to effectively anchor it to liposomes and emulsion. A peptide antigen B cell epitope from Group A Streptococcus M protein was conjugated to a universal T helper epitope, the pan DR-biding epitope (PADRE), alongside a lipidic moiety cholesterol...
February 18, 2024: Journal of Colloid and Interface Science
https://read.qxmd.com/read/38383848/a-novel-bioinformatic-method-for-the-identification-of-antimicrobial-peptides-in-metagenomes
#38
JOURNAL ARTICLE
Julianne Megaw, Timofey Skvortsov, Giulia Gori, Aliyu I Dabai, Brendan F Gilmore, Christopher C R Allen
AIMS: This study aimed to develop a new bioinformatic approach for the identification of novel antimicrobial peptides (AMPs), which did not depend on sequence similarity to known AMPs held within databases, but on structural mimicry of another antimicrobial compound, in this case an ultrashort, synthetic, cationic lipopeptide (C12-OOWW-NH2). METHODS AND RESULTS: When applied to a collection of metagenomic datasets, our outlined bioinformatic method successfully identified several short (8-10aa) functional AMPs, the activity of which was verified via disk diffusion and minimum inhibitory concentration (MIC) assays against a panel of 12 bacterial strains...
February 21, 2024: Journal of Applied Microbiology
https://read.qxmd.com/read/38378388/liposomal-co-encapsulation-of-a-novel-gemini-lipopeptide-and-a-cpg-odn-induces-a-strong-th1-response-with-the-co-activation-of-a-th2-th17-profile-and-high-antibody-levels
#39
JOURNAL ARTICLE
Ivana Gabriela Reidel, Cecilia Carol Curti, Léa Dorémus, Emile Béré, Adriana Delwail, Romina Cecilia Russi, Jean-Claude Lecron, Franck Morel, María Inés García, Diana María Müller, Jean-François Jégou, Carolina Melania Veaute
A successful vaccine depends on its capacity to elicit a protective immune response against the target pathogen. The adjuvant used plays an important role in enhancing and directing the immune response. Liposomes are vaccine adjuvants that allow the co-encapsulation of antigens and immunostimulants. Our aim was to evaluate the adjuvanticity of a cationic liposome (Lip) formulated with a novel gemini lipopeptide (AG2-C16) alone or in combination with CpG-ODN as immunostimulants. To achieve this, we used the recombinant clumping factor of Staphylococcus aureus (rClfA) as a model antigen, in a murine model...
February 19, 2024: Vaccine
https://read.qxmd.com/read/38376203/identification-of-clostridioides-difficile-mutants-with-increased-daptomycin-resistance
#40
JOURNAL ARTICLE
Brianne R Zbylicki, Claire E Murphy, Jennifer A Petsche, Ute Müh, Horia A Dobrila, Theresa D Ho, Mikaela N Daum, Anthony G Pannullo, David S Weiss, Craig D Ellermeier
Daptomycin is a cyclic lipopeptide antibiotic used to treat infections caused by some Gram-positive bacteria. Daptomycin disrupts synthesis of the peptidoglycan (PG) cell wall by inserting into the cytoplasmic membrane and binding multiple forms of the undecaprenyl carrier lipid required for PG synthesis. Membrane insertion requires phosphatidylglycerol, so studies of daptomycin can provide insight into assembly and maintenance of the cytoplasmic membrane. Here, we studied the effects of daptomycin on Clostridioides difficile , the leading cause of healthcare-associated diarrhea...
February 20, 2024: Journal of Bacteriology
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