Tsutomu Shinohara, Jan-Renier Moonen, Yoon Hong Chun, Yannick C Lee-Yow, Kenichi Okamura, Jason M Szafron, Jordan Kaplan, Aiqin Cao, Lingli Wang, Shalina Taylor, Sarasa Isobe, Melody Dong, Weiguang Yang, Katherine Guo, Benjamin D Franco, Cholawat Pacharinsak, Laura J Pisani, Shinji Saitoh, Yoshihide Mitani, Alison L Marsden, Jesse M Engreitz, Jakob Körbelin, Marlene Rabinovitch
Pathological high shear stress (HSS, 100 dyn/cm 2 ) is generated in distal pulmonary arteries (PA) (100-500 μm) in congenital heart defects and in progressive PA hypertension (PAH) with inward remodeling and luminal narrowing. Human PA endothelial cells (PAEC) were subjected to HSS versus physiologic laminar shear stress (LSS, 15 dyn/cm 2 ). Endothelial-mesenchymal transition (EndMT), a feature of PAH not previously attributed to HSS, was observed. H3K27ac peaks containing motifs for an ETS-family transcription factor (ERG) were reduced, as was ERG-Krüppel-like factors (KLF)2/4 interaction and ERG expression...
February 4, 2024: bioRxiv