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Mouse corpus cavernosum

Fábio H Silva, Serkan Karakus, Biljana Musicki, Hotaka Matsui, Trinity J Bivalacqua, Jean L Dos Santos, Fernando F Costa, Arthur L Burnett
Patients with sickle cell disease (SCD) display priapism, and dysregulated nitric oxide (NO) pathway may contribute to this condition. However, current therapies offered for the prevention of priapism in SCD are few. The 3-(1,3-dioxoisoindolin-2-yl)benzyl nitrate (compound 4C) was synthesized through molecular hybridization of hydroxyurea and thalidomide, which displays an NO-donor property. This study aimed to evaluate the effects of compound 4C on functional and molecular alterations of erectile function in murine models that display low NO bioavailability, SCD transgenic mice, and endothelial NO synthase and neuronal NO synthase double gene-deficient (dNOS(-/)) mice, focusing on the dysregulated NO-cGMP- phosphodiesterase type 5 (PDE5) pathway and oxidative stress in erectile tissue...
November 2016: Journal of Pharmacology and Experimental Therapeutics
Y D Xu, R L Guan, Y Y Wu, H E Lei, B C Yang, H X Li, L Wang, Y L Guo, Z C Xin
OBJECTIVE: To study the feasibility of transplantation of normal rat penile corpus cavernosum and major pelvic ganglion (MPG) into the renal subserous region of a Nu/Nu mouse based on allograft technology. METHODS: Penile corpus cavernosum and MPG, harvested from Sprague-Dawley (SD) rats under sterile condition, were transplanted underneath the kidney capsule of Nu/Nu mice through the microsurgery instruments and surgery microscope. The histopathologic changes and cellular proliferation in the transplanted penile corpus cavernosum and MPG were then analyzed at the end of 1week and 4 weeks after transplantation...
February 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
Thiago Gagliano-Jucá, Mauro Napolitano, Fernanda Del Grossi Ferraz Carvalho, Rafael Campos, Fabíola Zakia Mónica, Mário Angelo Claudino, Edson Antunes, Anibal Gil Lopes, Gilberto De Nucci
INTRODUCTION: Hydrochlorothiazide has a negative influence on penile erection but little is known about the mechanism(s) involved. AIMS: To characterize the effects of this diuretic on mouse corpus cavernosum (CC) smooth muscle in vitro and ex vivo. METHODS: CC strips of C57BL/6 mice (12-16 weeks old) were mounted in organ baths containing Krebs-Henseleit solution and tissue reactivity was evaluated. Expression of genes encoding diuretic targets and enzymes involved in penile erection were evaluated by polymerase chain reaction...
June 2016: Sexual Medicine
Charlotte Boydens, Bart Pauwels, Laura Vanden Daele, Johan Van de Voorde
BACKGROUND: Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Resveratrol and quercetin are red wine polyphenols with known beneficial cardiovascular properties, including an antioxidant capacity. This study evaluated whether resveratrol and/or quercetin could prevent in vitro-induced diabetic changes in neurogenic and vascular relaxant responses of mouse arteries and corpora cavernosa. METHODS: Isometric tension of isolated aorta, mesenteric arteries and corpora cavernosa was measured using organ bath systems...
2016: Cardiovascular Diabetology
Fatma Aydinoglu, Nuran Ogulener
The aim of this study was to investigate the mechanism of H2 S-induced relaxation in mouse corpus cavernosal tissue. l-cysteine (10(-6) × 10(-3) mol/L) and exogenous H2 S (NaHS; 10(-6) to 10(-3) mol/L) induced concentration-dependent relaxation. l-cysteine-induced relaxations was reduced by d,l-propargylglycine, a cystathionine gamma lyase (CSE) inhibitor but not influenced by aminooxyacetic acid, a cystathionine beta synthase (CBS) inhibitor. l-cysteine induced relaxations, but not of those of H2 S diminished in endothelium-denuded tissues...
April 2016: Clinical and Experimental Pharmacology & Physiology
Dulguun Batbold, Kang-Moon Song, Jin-Mi Park, Soo-Hwan Park, Tack Lee, Dong-Soo Ryu, Young-Ger Suh, Young-Guen Kwon, Ji-Kan Ryu, Jun-Kyu Suh
PURPOSE: We examined whether and how Sac-1004, a vascular leakage blocker, would restore erectile function in an animal model of diabetic erectile dysfunction. MATERIALS AND METHODS: Eight-week-old C57BL/6J mice were used. Diabetes was induced by intraperitoneal injection of streptozotocin. Eight weeks after diabetes induction the animals were divided into 6 groups, including controls, diabetic mice that received repeat intracavernous injections of phosphate buffered saline (20 μl) on days -3 and 0, and diabetic mice that received repeat intracavernous injections of Sac-1004 on days -3 and 0 (1, 2, 5 and 10 μg, respectively, in 20 μl phosphate buffered saline)...
June 2016: Journal of Urology
J-K Ryu, D-H Kim, K-M Song, D-S Ryu, S-N Kim, D-H Shin, T Yi, J-K Suh, S U Song
The major hurdle for the clinical application of stem cell therapy is the heterogeneous nature of the isolated cells, which may cause different treatment outcomes. The aim of this study was to examine the effectiveness of mouse clonal bone marrow-derived stem cells (BMSCs) obtained from a single colony by using subfractionation culturing method for erectile function in diabetic animals. Twelve-week-old C57BL/6J mice were divided into four groups: controls, diabetic mice, and diabetic mice treated with a single intracavernous injection of PBS (20 μL) or clonal BMSCs (3 × 10(5) cells/20 μL)...
January 2016: Andrology
Guo Nan Yin, Nando Dulal Das, Min Ji Choi, Kang-Moon Song, Mi-Hye Kwon, Jiyeon Ock, Anita Limanjaya, Kalyan Ghatak, Woo Jean Kim, Jae Seog Hyun, Gou Young Koh, Ji-Kan Ryu, Jun-Kyu Suh
Pericytes are known to play critical roles in vascular development and homeostasis. However, the distribution of cavernous pericytes and their roles in penile erection is unclear. Herein we report that the pericytes are abundantly distributed in microvessels of the subtunical area and dorsal nerve bundle of mice, followed by dorsal vein and cavernous sinusoids. We further confirmed the presence of pericytes in human corpus cavernosum tissue and successfully isolated pericytes from mouse penis. Cavernous pericyte contents from diabetic mice and tube formation of cultured pericytes in high glucose condition were greatly reduced compared with those in normal conditions...
2015: Scientific Reports
Kang-Moon Song, Min Ji Choi, Mi-Hye Kwon, Kalyan Ghatak, Soo-Hwan Park, Dong-Soo Ryu, Ji-Kan Ryu, Jun-Kyu Suh
PURPOSE: Electroporation is known to enhance the efficiency of gene transfer through a transient increase in cell membrane permeability. The aim of this study was to determine the optimal conditions for in vivo electroporation-mediated gene delivery into mouse corpus cavernosum. MATERIALS AND METHODS: Diabetes was induced in C57BL/6 mice by intraperitoneal injections of streptozotocin. After intracavernous injection of pCMV-Luc (100 µg/40 µL), different electroporation settings (5-50 V, 8-16 pulses with a duration of 40-100 ms) were applied to the penis to establish the optimal conditions for electroporation...
March 2015: Korean Journal of Urology
Fatma Peyman Ertuğ, Erğin Singirik, Hacer Sinem Büyüknacar, Cemil Göçmen, Mehmet Ata Seçilmiş
BACKGROUND/AIM: To investigate the effects of spermine NONOate in the cavernous tissue obtained from mice treated or untreated with sildenafil. MATERIALS AND METHODS: We studied the effects of spermine NONOate on the tone and nitrergic relaxation responses of isolated mouse corpus cavernosum and compared them with sodium nitroprusside in the absence or presence of L-nitroarginine, hydroxocobalamin, pyrogallol, diethyldithiocarbamate (DETCA), or 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ)...
2014: Turkish Journal of Medical Sciences
Abdulmaged M Traish, Casey Stottrup, Koenraad van Renterghem, Ruth Achten, Sayon Roy
AIM: Altered expression of connexin 43 (Cx43) has been postulated to be involved in the development and progression of various diseases including erectile dysfunction (ED). The aim of this study was to determine whether distribution and density of the gap junction protein Cx43 are altered in human corpus cavernosum (HCC) tissue samples derived from diabetic or hypogonadal patients with ED compared to those from normal subjects. METHODS: HCC tissue sections derived from normal, diabetic and hypogonadal subjects were fixed in 4% formaldehyde, embedded in paraffin and immunostained with a monoclonal mouse anti-rat Cx43 antibody...
June 2013: Hormone Molecular Biology and Clinical Investigation
Rodrigo A Fraga-Silva, Fabiana P Costa-Fraga, Fabrizio Montecucco, Mikael Sturny, Younoss Faye, François Mach, Graziano Pelli, Vinayak Shenoy, Rafaela F da Silva, Mohan K Raizada, Robson A S Santos, Nikolaos Stergiopulos
INTRODUCTION: Angiotensin-converting enzyme 2 (ACE2) is a key enzyme of the renin angiotensin system, which breaks down angiotensin II and forms angiotensin-(1-7). In erectile tissues, it has been documented that angiotensin II contributes to the development of erectile dysfunction (ED), while treatment with angiotensin-(1-7) improves penile erection. However, the expression and function of ACE2 in erectile tissues have never been investigated. AIM: Here, we examined the expression of ACE2 in erectile tissues and its actions against hypercholesterolemia-induced corpus cavernosum (CC) injury...
February 2015: Journal of Sexual Medicine
Neha Singh, Goutham Vasam, Rahul Pawar, Yagna P R Jarajapu
INTRODUCTION: Angiotensin (Ang)-(1-7) is a recently identified vasoprotective heptapeptide, and it appears to activate the reparative functions of bone marrow-derived stem/progenitor cells (BMPCs). AIM: This study evaluated the effect of Ang-(1-7) in the angiogenic function of cavernosum in type 1 diabetes (T1D) and delineated the role of BMPCs in this protective function. METHODS: T1D was induced by streptozotocin in mice, and mice with 20-24 weeks of diabetes were used for the study...
September 2014: Journal of Sexual Medicine
Kang-Moon Song, Hai-Rong Jin, Jin-Mi Park, Min Ji Choi, Mi-Hye Kwon, Ki-Dong Kwon, Dulguun Batbold, Guo Nan Yin, Woo Jean Kim, Gou Young Koh, Ji-Kan Ryu, Jun-Kyu Suh
INTRODUCTION: Erectile dysfunction (ED) is a major complication of radical prostatectomy. Men with radical prostatectomy-induced ED respond less positively to oral phosphodiesterase-5 inhibitors. AIM: The study aims to examine whether and how stromal vascular fraction (SVF) restores erectile function in mice with cavernous nerve injury (CNI). METHODS: Twelve-week-old male C57BL/6J mice were used and the animals were distributed into five groups: sham operation group and CNI group receiving a single intracavernous injection of phosphate-buffered saline (PBS) or SVF (1 × 10(4) , 1 × 10(5) , or 3 × 10(5) cells/20 μL, respectively)...
August 2014: Journal of Sexual Medicine
A Lafuente-Sanchis, D Triguero, A Garcia-Pascual
Erectile dysfunction (ED) is very prevalent in the older population, although the ageing-related mechanisms involved in the development of ED are poorly understood. We propose that age-induced differences in nerve- and endothelium-mediated smooth muscle contractility in the corpus cavernosum (CC) could be found between a senescent-accelerated mouse prone (SAMP8) and senescent-accelerated mouse resistant (SAMR1) strains. We analysed the changes in muscle tension induced by electrical field stimulation (EFS) or agonist addition 'in vitro', assessing nerve density (adrenergic, cholinergic and nitrergic), the expression of endothelial nitric oxide synthase (eNOS), cGMP accumulation and the distribution of interstitial cells (ICs) by immunofluorescence...
July 2014: Andrology
Kang Moon Song, Jae-Seung Chung, Min Ji Choi, Hai-Rong Jin, Guo Nan Yin, Mi-Hye Kwon, Jin-Mi Park, Woo Jean Kim, Sang-Jin Lee, Seong-Jin Kim, Ji-Kan Ryu, Jun-Kyu Suh
INTRODUCTION: Men with erectile dysfunction (ED) respond poorly to oral phosphodiesterase-5 inhibitors following radical prostatectomy. Recent studies have reported that up-regulation of transforming growth factor-β1 (TGF-β1) and activation of the Smad signaling pathway play important roles in cavernous fibrosis and in the deterioration of erectile function in a mouse model of cavernous nerve injury (CNI) and in patients with spinal cord injury. The mothers against decapentaplegic homolog 7 (Smad7) is known to inhibit the phosphorylation of Smad2 and Smad3...
January 2014: Journal of Sexual Medicine
S H Shin, W J Kim, M J Choi, J-M Park, H-R Jin, G N Yin, J-K Ryu, J-K Suh
Diabetic erectile dysfunction (ED) has multiple causative factors, such as endothelial and smooth muscle dysfunction and cavernous fibrosis. Wnt signalling is essential for normal embryonic development and for tissue homeostasis in adults. Aberrant activation of Wnt family members has been implicated in tissue fibrosis and in angiogenesis. In this study, we investigated the differential expression of Wnts in the penises of mice with streptozotocin-induced diabetic ED. We also examined the effect of transforming growth factor-β1 (TGF-β1) on the expression of Wnts in primary cultured fibroblasts isolated from human tunica albuginea...
January 2014: Andrology
Z-H Liu, L-P Yu, T Xu, X-W Zhang, Y-Q Yuan, Y-B Xiao, J Li, Y-C Hao, Y-P Zhao, X-F Wang
Metabolic syndrome is closely related to erectile dysfunction (ED), and hyperlipidaemia is considered a major risk factor for ED. Adenosine triphosphate (ATP) synthase is believed to play an important role in metabolic syndrome; it has been hypothesised that ATP synthase contributes to ED development. We have verified this hypothesis using primary cultured human corpus cavernosum smooth muscle (HCCSM) cells treated with excessive free fat acid (FFA) and a high-fat diet (HFD) mouse model. Our results showed that high fatty factors could cause lipid accumulation in HCCSM cells, which could result in abnormal lipid metabolism, such as high levels of triglycerides, cholesterol and glucose in the HFD mice...
June 2014: Andrologia
J-K Ryu, M J Choi, T-I Kim, H-R Jin, K-D Kwon, D Batbold, K-M Song, M-H Kwon, G N Yin, M Lee, S W Kim, J-K Suh
A prerequisite for the successful clinical application of gene therapy in erectile dysfunction (ED) is the availability of safe and efficient gene delivery systems. The aim of this study was to examine the effectiveness of guanidinylated bioreducible polymer (GBP) polyplexes for gene delivery systems, which take advantage of the biodegradability of reducible disulfide bonds and the cell-penetrating ability of guanidine groups. For in vitro transfection experiments, we used mouse cavernous endothelial cells and A7r5 rat vascular smooth muscle cells...
March 2013: Andrology
Ji-Kan Ryu, Munkhbayar Tumurbaatar, Hai-Rong Jin, Woo Jean Kim, Mi-Hye Kwon, Shuguang Piao, Min Ji Choi, Guo Nan Yin, Kang-Moon Song, Yong-Jin Kang, Young Jun Koh, Gou Young Koh, Jun-Kyu Suh
INTRODUCTION: Men with diabetic erectile dysfunction (ED) often have severe endothelial dysfunction and respond poorly to oral phosphodiesterase-5 inhibitors. AIM: To examine whether and how freshly isolated stromal vascular fraction (SVF) promotes cavernous endothelial regeneration and restores erectile function in diabetic animals. METHODS: Eight-week-old C57BL/6J mice were used. Diabetes was induced by intraperitoneal injection of streptozotocin...
December 2012: Journal of Sexual Medicine
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